Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013126.t1 | XP_003602846.1 | 92.7 | 192 | 8 | 2 | 1 | 192 | 1 | 186 | 2.40E-55 | 225.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013126.t1 | Q41001 | 83.6 | 122 | 17 | 3 | 1 | 120 | 1 | 121 | 5.7e-50 | 198.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013126.t1 | B7FHG1 | 93.2 | 192 | 8 | 2 | 1 | 192 | 1 | 187 | 1.7e-55 | 225.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene074086 | MS.gene013126 | PPI |
| MS.gene47150 | MS.gene013126 | PPI |
| MS.gene55509 | MS.gene013126 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013126.t1 | MTR_3g099580 | 86.458 | 192 | 20 | 2 | 1 | 192 | 1 | 186 | 3.11e-93 | 270 |
| MS.gene013126.t1 | MTR_3g099570 | 77.083 | 192 | 36 | 3 | 1 | 192 | 1 | 184 | 9.47e-92 | 266 |
| MS.gene013126.t1 | MTR_3g099680 | 60.909 | 220 | 43 | 4 | 7 | 188 | 6 | 220 | 4.34e-70 | 212 |
| MS.gene013126.t1 | MTR_3g099980 | 61.468 | 218 | 43 | 4 | 7 | 188 | 6 | 218 | 1.68e-69 | 211 |
| MS.gene013126.t1 | MTR_3g099540 | 67.717 | 127 | 41 | 0 | 1 | 127 | 1 | 127 | 1.10e-57 | 177 |
| MS.gene013126.t1 | MTR_8g007020 | 47.027 | 185 | 88 | 6 | 7 | 186 | 4 | 183 | 5.08e-40 | 135 |
| MS.gene013126.t1 | MTR_8g007035 | 56.098 | 123 | 50 | 3 | 5 | 124 | 2 | 123 | 8.48e-40 | 134 |
| MS.gene013126.t1 | MTR_1g090190 | 35.928 | 167 | 100 | 2 | 24 | 190 | 20 | 179 | 2.15e-32 | 115 |
| MS.gene013126.t1 | MTR_5g006040 | 39.326 | 178 | 95 | 8 | 13 | 183 | 10 | 181 | 4.19e-29 | 106 |
| MS.gene013126.t1 | MTR_2g088990 | 41.781 | 146 | 77 | 4 | 13 | 152 | 12 | 155 | 3.92e-28 | 105 |
| MS.gene013126.t1 | MTR_4g114870 | 50.833 | 120 | 51 | 4 | 25 | 138 | 27 | 144 | 6.30e-28 | 107 |
| MS.gene013126.t1 | MTR_4g114870 | 50.000 | 100 | 45 | 2 | 27 | 121 | 191 | 290 | 2.19e-25 | 101 |
| MS.gene013126.t1 | MTR_6g080660 | 48.000 | 100 | 52 | 0 | 27 | 126 | 28 | 127 | 1.36e-27 | 103 |
| MS.gene013126.t1 | MTR_1g112700 | 34.694 | 147 | 85 | 4 | 3 | 138 | 7 | 153 | 1.46e-25 | 97.8 |
| MS.gene013126.t1 | MTR_0334s0010 | 39.516 | 124 | 72 | 1 | 3 | 123 | 4 | 127 | 2.76e-25 | 97.4 |
| MS.gene013126.t1 | MTR_1g104800 | 31.016 | 187 | 124 | 5 | 1 | 184 | 1 | 185 | 2.81e-24 | 94.4 |
| MS.gene013126.t1 | MTR_6g023760 | 44.118 | 102 | 56 | 1 | 21 | 121 | 20 | 121 | 1.39e-23 | 92.4 |
| MS.gene013126.t1 | MTR_4g067200 | 39.552 | 134 | 74 | 4 | 3 | 129 | 4 | 137 | 2.96e-23 | 91.3 |
| MS.gene013126.t1 | MTR_4g066110 | 39.552 | 134 | 74 | 4 | 3 | 129 | 4 | 137 | 2.96e-23 | 91.3 |
| MS.gene013126.t1 | MTR_7g086140 | 41.860 | 129 | 66 | 4 | 1 | 124 | 1 | 125 | 5.02e-23 | 90.5 |
| MS.gene013126.t1 | MTR_7g086190 | 41.860 | 129 | 66 | 4 | 1 | 124 | 1 | 125 | 5.02e-23 | 90.5 |
| MS.gene013126.t1 | MTR_7g086160 | 38.889 | 126 | 75 | 2 | 1 | 124 | 1 | 126 | 5.28e-22 | 87.8 |
| MS.gene013126.t1 | MTR_1g105130 | 39.474 | 114 | 64 | 3 | 13 | 121 | 8 | 121 | 1.72e-21 | 86.3 |
| MS.gene013126.t1 | MTR_1g105120 | 39.474 | 114 | 64 | 3 | 13 | 121 | 8 | 121 | 1.72e-21 | 86.3 |
| MS.gene013126.t1 | MTR_6g083240 | 32.632 | 190 | 100 | 6 | 8 | 190 | 8 | 176 | 2.70e-21 | 86.3 |
| MS.gene013126.t1 | MTR_8g099220 | 41.748 | 103 | 56 | 2 | 24 | 122 | 26 | 128 | 4.94e-21 | 84.3 |
| MS.gene013126.t1 | MTR_7g086220 | 36.434 | 129 | 72 | 4 | 1 | 124 | 1 | 124 | 9.16e-20 | 82.0 |
| MS.gene013126.t1 | MTR_7g086090 | 38.400 | 125 | 71 | 3 | 1 | 122 | 1 | 122 | 1.10e-19 | 83.6 |
| MS.gene013126.t1 | MTR_7g086230 | 37.398 | 123 | 75 | 2 | 1 | 122 | 1 | 122 | 2.47e-19 | 81.3 |
| MS.gene013126.t1 | MTR_2g025580 | 36.029 | 136 | 83 | 3 | 16 | 147 | 16 | 151 | 3.07e-19 | 80.9 |
| MS.gene013126.t1 | MTR_1g090420 | 32.022 | 178 | 101 | 5 | 1 | 158 | 2 | 179 | 3.26e-19 | 82.0 |
| MS.gene013126.t1 | MTR_7g086100 | 38.525 | 122 | 73 | 2 | 1 | 121 | 1 | 121 | 3.70e-19 | 82.0 |
| MS.gene013126.t1 | MTR_4g081100 | 39.316 | 117 | 66 | 2 | 10 | 121 | 15 | 131 | 5.70e-19 | 81.3 |
| MS.gene013126.t1 | MTR_2g043730 | 43.000 | 100 | 53 | 3 | 22 | 120 | 27 | 123 | 1.12e-18 | 78.2 |
| MS.gene013126.t1 | MTR_8g463180 | 35.075 | 134 | 79 | 4 | 12 | 141 | 8 | 137 | 2.52e-18 | 80.9 |
| MS.gene013126.t1 | MTR_7g086280 | 36.585 | 123 | 76 | 2 | 1 | 122 | 1 | 122 | 3.65e-18 | 78.2 |
| MS.gene013126.t1 | MTR_2g083250 | 42.157 | 102 | 52 | 2 | 29 | 123 | 28 | 129 | 4.99e-18 | 79.7 |
| MS.gene013126.t1 | MTR_4g077787 | 28.485 | 165 | 108 | 3 | 2 | 160 | 7 | 167 | 2.72e-17 | 76.3 |
| MS.gene013126.t1 | MTR_4g111640 | 28.977 | 176 | 90 | 5 | 13 | 182 | 24 | 170 | 3.25e-17 | 75.9 |
| MS.gene013126.t1 | MTR_3g089035 | 43.299 | 97 | 51 | 3 | 24 | 119 | 30 | 123 | 3.96e-17 | 74.3 |
| MS.gene013126.t1 | MTR_6g013420 | 36.842 | 133 | 74 | 6 | 8 | 136 | 20 | 146 | 6.24e-17 | 75.1 |
| MS.gene013126.t1 | MTR_2g101300 | 34.641 | 153 | 88 | 4 | 7 | 149 | 3 | 153 | 6.30e-17 | 75.1 |
| MS.gene013126.t1 | MTR_2g090580 | 36.691 | 139 | 77 | 4 | 29 | 159 | 29 | 164 | 7.17e-17 | 75.5 |
| MS.gene013126.t1 | MTR_4g007250 | 38.835 | 103 | 61 | 2 | 21 | 122 | 23 | 124 | 2.47e-16 | 73.2 |
| MS.gene013126.t1 | MTR_6g013200 | 30.409 | 171 | 100 | 6 | 1 | 167 | 8 | 163 | 4.73e-16 | 72.4 |
| MS.gene013126.t1 | MTR_6g022170 | 37.864 | 103 | 58 | 3 | 24 | 121 | 31 | 132 | 9.67e-16 | 71.6 |
| MS.gene013126.t1 | MTR_4g078410 | 32.258 | 155 | 100 | 4 | 29 | 180 | 27 | 179 | 1.07e-15 | 72.0 |
| MS.gene013126.t1 | MTR_2g090575 | 36.364 | 121 | 70 | 3 | 8 | 121 | 2 | 122 | 1.14e-15 | 74.3 |
| MS.gene013126.t1 | MTR_4g112310 | 28.169 | 142 | 79 | 2 | 41 | 182 | 2 | 120 | 5.06e-15 | 68.9 |
| MS.gene013126.t1 | MTR_2g043790 | 41.414 | 99 | 54 | 3 | 22 | 119 | 2 | 97 | 1.74e-14 | 66.6 |
| MS.gene013126.t1 | MTR_1g014120 | 38.614 | 101 | 58 | 2 | 42 | 138 | 45 | 145 | 3.27e-14 | 67.8 |
| MS.gene013126.t1 | MTR_3g105930 | 35.714 | 98 | 62 | 1 | 42 | 138 | 47 | 144 | 3.40e-14 | 67.8 |
| MS.gene013126.t1 | MTR_4g130780 | 30.714 | 140 | 91 | 3 | 1 | 134 | 3 | 142 | 6.58e-14 | 67.0 |
| MS.gene013126.t1 | MTR_8g086360 | 31.250 | 192 | 115 | 9 | 12 | 191 | 5 | 191 | 1.01e-13 | 66.6 |
| MS.gene013126.t1 | MTR_8g094990 | 29.381 | 194 | 109 | 7 | 8 | 174 | 2 | 194 | 1.25e-13 | 67.8 |
| MS.gene013126.t1 | MTR_4g130800 | 34.375 | 128 | 76 | 5 | 1 | 121 | 3 | 129 | 1.52e-13 | 67.4 |
| MS.gene013126.t1 | MTR_4g114830 | 37.037 | 108 | 59 | 5 | 18 | 116 | 18 | 125 | 1.58e-13 | 65.5 |
| MS.gene013126.t1 | MTR_8g095013 | 31.250 | 176 | 100 | 6 | 8 | 166 | 2 | 173 | 1.65e-13 | 67.8 |
| MS.gene013126.t1 | MTR_4g124280 | 31.792 | 173 | 96 | 5 | 24 | 174 | 22 | 194 | 5.28e-13 | 65.5 |
| MS.gene013126.t1 | MTR_8g088830 | 39.000 | 100 | 52 | 5 | 24 | 119 | 25 | 119 | 5.29e-13 | 63.2 |
| MS.gene013126.t1 | MTR_4g130800 | 40.506 | 79 | 46 | 1 | 44 | 121 | 50 | 128 | 6.21e-13 | 65.9 |
| MS.gene013126.t1 | MTR_8g095020 | 33.557 | 149 | 79 | 5 | 28 | 162 | 26 | 168 | 9.42e-13 | 65.9 |
| MS.gene013126.t1 | MTR_5g088170 | 33.929 | 112 | 68 | 4 | 30 | 138 | 20 | 128 | 1.72e-11 | 60.1 |
| MS.gene013126.t1 | MTR_5g088180 | 33.929 | 112 | 68 | 4 | 30 | 138 | 33 | 141 | 2.17e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene013126.t1 | AT1G72230 | 46.809 | 188 | 84 | 4 | 3 | 183 | 1 | 179 | 8.10e-49 | 157 |
| MS.gene013126.t1 | AT1G22480 | 64.130 | 92 | 33 | 0 | 35 | 126 | 27 | 118 | 3.86e-36 | 124 |
| MS.gene013126.t1 | AT2G32300 | 43.651 | 126 | 70 | 1 | 4 | 128 | 3 | 128 | 8.90e-30 | 110 |
| MS.gene013126.t1 | AT2G32300 | 43.651 | 126 | 70 | 1 | 4 | 128 | 33 | 158 | 3.44e-29 | 109 |
| MS.gene013126.t1 | AT3G60270 | 47.115 | 104 | 55 | 0 | 23 | 126 | 22 | 125 | 5.29e-28 | 104 |
| MS.gene013126.t1 | AT5G07475 | 38.926 | 149 | 81 | 3 | 24 | 172 | 27 | 165 | 1.10e-26 | 100 |
| MS.gene013126.t1 | AT3G27200 | 41.129 | 124 | 67 | 3 | 1 | 121 | 1 | 121 | 2.05e-26 | 99.8 |
| MS.gene013126.t1 | AT2G26720 | 34.807 | 181 | 104 | 5 | 25 | 192 | 27 | 206 | 1.79e-25 | 98.2 |
| MS.gene013126.t1 | AT2G31050 | 45.217 | 115 | 58 | 3 | 25 | 137 | 27 | 138 | 2.47e-25 | 97.4 |
| MS.gene013126.t1 | AT2G44790 | 50.526 | 95 | 45 | 1 | 35 | 127 | 36 | 130 | 4.13e-25 | 97.1 |
| MS.gene013126.t1 | AT5G26330 | 44.554 | 101 | 53 | 2 | 24 | 121 | 21 | 121 | 9.67e-24 | 93.2 |
| MS.gene013126.t1 | AT3G17675 | 38.095 | 105 | 64 | 1 | 18 | 121 | 23 | 127 | 3.69e-21 | 84.7 |
| MS.gene013126.t1 | AT3G60280 | 42.157 | 102 | 59 | 0 | 23 | 124 | 20 | 121 | 1.63e-20 | 85.5 |
| MS.gene013126.t1 | AT5G53870 | 32.620 | 187 | 103 | 5 | 7 | 175 | 7 | 188 | 3.92e-18 | 81.3 |
| MS.gene013126.t1 | AT5G53870 | 32.620 | 187 | 103 | 5 | 7 | 175 | 7 | 188 | 6.85e-18 | 80.5 |
| MS.gene013126.t1 | AT5G20230 | 36.047 | 172 | 91 | 5 | 27 | 179 | 25 | 196 | 1.23e-17 | 77.4 |
| MS.gene013126.t1 | AT4G31840 | 33.803 | 142 | 86 | 4 | 3 | 136 | 1 | 142 | 4.21e-17 | 75.5 |
| MS.gene013126.t1 | AT2G25060 | 37.719 | 114 | 65 | 3 | 28 | 135 | 32 | 145 | 7.39e-17 | 75.1 |
| MS.gene013126.t1 | AT3G18590 | 34.646 | 127 | 76 | 2 | 1 | 121 | 1 | 126 | 1.44e-16 | 74.3 |
| MS.gene013126.t1 | AT4G28365 | 31.928 | 166 | 102 | 6 | 29 | 183 | 31 | 196 | 7.67e-16 | 72.8 |
| MS.gene013126.t1 | AT1G64640 | 37.705 | 122 | 69 | 3 | 24 | 138 | 30 | 151 | 1.04e-15 | 72.0 |
| MS.gene013126.t1 | AT5G15350 | 36.364 | 121 | 70 | 4 | 8 | 122 | 7 | 126 | 3.06e-15 | 70.5 |
| MS.gene013126.t1 | AT5G57920 | 37.113 | 97 | 59 | 2 | 42 | 137 | 43 | 138 | 4.93e-15 | 70.1 |
| MS.gene013126.t1 | AT5G57920 | 37.113 | 97 | 59 | 2 | 42 | 137 | 75 | 170 | 5.13e-15 | 70.9 |
| MS.gene013126.t1 | AT4G27520 | 34.722 | 144 | 83 | 4 | 10 | 142 | 9 | 152 | 5.77e-15 | 72.4 |
| MS.gene013126.t1 | AT5G57920 | 37.113 | 97 | 59 | 2 | 42 | 137 | 42 | 137 | 7.64e-15 | 69.7 |
| MS.gene013126.t1 | AT3G53330 | 32.540 | 126 | 78 | 4 | 23 | 144 | 184 | 306 | 2.09e-14 | 70.5 |
| MS.gene013126.t1 | AT4G30590 | 33.673 | 98 | 64 | 1 | 42 | 138 | 48 | 145 | 4.68e-14 | 67.8 |
| MS.gene013126.t1 | AT4G12880 | 34.167 | 120 | 72 | 5 | 7 | 122 | 10 | 126 | 7.07e-14 | 66.2 |
| MS.gene013126.t1 | AT1G45063 | 36.207 | 116 | 63 | 5 | 26 | 135 | 148 | 258 | 1.04e-13 | 68.2 |
| MS.gene013126.t1 | AT1G45063 | 39.216 | 102 | 54 | 4 | 26 | 121 | 148 | 247 | 1.20e-13 | 68.6 |
| MS.gene013126.t1 | AT5G14345 | 30.469 | 128 | 81 | 3 | 13 | 133 | 2 | 128 | 1.71e-13 | 65.1 |
| MS.gene013126.t1 | AT3G20570 | 33.721 | 172 | 102 | 5 | 9 | 171 | 15 | 183 | 2.98e-13 | 65.9 |
| MS.gene013126.t1 | AT1G48940 | 37.755 | 98 | 54 | 3 | 30 | 121 | 29 | 125 | 5.82e-13 | 64.3 |
| MS.gene013126.t1 | AT4G32490 | 30.841 | 107 | 71 | 2 | 29 | 132 | 33 | 139 | 1.16e-12 | 64.3 |
| MS.gene013126.t1 | AT2G02850 | 39.000 | 100 | 51 | 4 | 24 | 119 | 33 | 126 | 1.81e-12 | 62.0 |
| MS.gene013126.t1 | AT1G79800 | 33.333 | 117 | 67 | 3 | 29 | 135 | 36 | 151 | 1.99e-12 | 63.2 |
| MS.gene013126.t1 | AT2G23990 | 33.684 | 95 | 61 | 2 | 42 | 134 | 47 | 141 | 2.93e-12 | 63.2 |
| MS.gene013126.t1 | AT5G25090 | 32.258 | 124 | 77 | 3 | 7 | 125 | 9 | 130 | 1.98e-11 | 60.5 |
| MS.gene013126.t1 | AT4G01380 | 31.193 | 109 | 65 | 4 | 33 | 133 | 78 | 184 | 4.56e-11 | 60.1 |
| MS.gene013126.t1 | AT3G01070 | 38.462 | 117 | 66 | 3 | 9 | 122 | 12 | 125 | 6.03e-11 | 58.9 |
Find 55 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTCTTAGCAATTAATA+TGG | 0.256926 | 3.1:-79603723 | MS.gene013126:CDS |
| AGTGGTGGTGGTGGAGGGTG+TGG | 0.258082 | 3.1:+79603128 | None:intergenic |
| GGTGTGGTGGTGGTTGCAGC+AGG | 0.286767 | 3.1:+79603144 | None:intergenic |
| GGTGGTGGAGGGTGTGGTGG+TGG | 0.288673 | 3.1:+79603134 | None:intergenic |
| GGTGGTGGTGGAGGGTGTGG+TGG | 0.299929 | 3.1:+79603131 | None:intergenic |
| GGAAGGAGTGGTGGTGGTGG+AGG | 0.314330 | 3.1:+79603122 | None:intergenic |
| GATCTTGATCAGTTATATAT+TGG | 0.317167 | 3.1:-79603034 | MS.gene013126:CDS |
| GAAACTTTCTGTTAAGGTTA+AGG | 0.337776 | 3.1:-79603241 | MS.gene013126:CDS |
| TACTTCATATGTGCTGTTCC+TGG | 0.349993 | 3.1:-79603282 | MS.gene013126:CDS |
| GATAAATCAGGTTGGGCAAT+TGG | 0.365257 | 3.1:-79603665 | MS.gene013126:CDS |
| GACTATAAATCATGCACAAC+TGG | 0.368543 | 3.1:-79603369 | MS.gene013126:CDS |
| GAAGGAGTGGTGGTGGTGGA+GGG | 0.390042 | 3.1:+79603123 | None:intergenic |
| TGTGGAAGGAGTGGTGGTGG+TGG | 0.402692 | 3.1:+79603119 | None:intergenic |
| TGGCATGAAACTTTCTGTTA+AGG | 0.430118 | 3.1:-79603247 | MS.gene013126:CDS |
| TGTGGTGGTGGTTGCAGCAG+GGG | 0.433694 | 3.1:+79603146 | None:intergenic |
| ACTATGACAGTGTTCAACTA+TGG | 0.440045 | 3.1:-79603426 | MS.gene013126:intron |
| TCCTGCTTTCTTGAGAGGGA+TGG | 0.445617 | 3.1:+79603311 | None:intergenic |
| TCTGCAACACCATCACCATC+AGG | 0.461815 | 3.1:-79603192 | MS.gene013126:CDS |
| GTGTGGTGGTGGTTGCAGCA+GGG | 0.463658 | 3.1:+79603145 | None:intergenic |
| ATGCCACCTGTGCAATGTCC+AGG | 0.481287 | 3.1:+79603264 | None:intergenic |
| GTGTTCCTGCTTTCTTGAGA+GGG | 0.482705 | 3.1:+79603307 | None:intergenic |
| TGTGTTCCTGCTTTCTTGAG+AGG | 0.493116 | 3.1:+79603306 | None:intergenic |
| TTTCTTGAGAGGGATGGTTG+TGG | 0.493786 | 3.1:+79603317 | None:intergenic |
| CACACCGTTGGAGATAAATC+AGG | 0.494660 | 3.1:-79603677 | MS.gene013126:CDS |
| GGCTAGTGACAAAACATTTG+CGG | 0.495560 | 3.1:-79603624 | MS.gene013126:CDS |
| TTTGCGGTTGGTGACAGTCT+TGG | 0.500707 | 3.1:-79603608 | MS.gene013126:intron |
| CGTTGGAGATAAATCAGGTT+GGG | 0.514147 | 3.1:-79603672 | MS.gene013126:CDS |
| GGTTGCAGCAGGGGTGCCAT+CGG | 0.524569 | 3.1:+79603155 | None:intergenic |
| AAGAAGCCTAAAATCAAAGA+AGG | 0.528032 | 3.1:+79603740 | None:intergenic |
| CTTGCAACTGTCCACACCGT+TGG | 0.529565 | 3.1:-79603689 | MS.gene013126:CDS |
| TTGTGTGGAAGGAGTGGTGG+TGG | 0.529906 | 3.1:+79603116 | None:intergenic |
| GCAGCAGGGGTGCCATCGGA+AGG | 0.553762 | 3.1:+79603159 | None:intergenic |
| ACCATCCCTCTCAAGAAAGC+AGG | 0.564418 | 3.1:-79603312 | MS.gene013126:CDS |
| GAACCTTTCCCTGATGGTGA+TGG | 0.566964 | 3.1:+79603183 | None:intergenic |
| GTTCCTGGACATTGCACAGG+TGG | 0.574513 | 3.1:-79603267 | MS.gene013126:CDS |
| GAAGGTGAACCTTTCCCTGA+TGG | 0.578557 | 3.1:+79603177 | None:intergenic |
| ACAGTGTTCAACTATGGTGC+TGG | 0.581813 | 3.1:-79603420 | MS.gene013126:intron |
| CCAACCTGATTTATCTCCAA+CGG | 0.588831 | 3.1:+79603673 | None:intergenic |
| GTGTGGACAGTTGCAAGAGT+TGG | 0.591396 | 3.1:+79603695 | None:intergenic |
| AGTGACAAAACATTTGCGGT+TGG | 0.594495 | 3.1:-79603620 | MS.gene013126:CDS |
| GCTGTTCCTGGACATTGCAC+AGG | 0.594776 | 3.1:-79603270 | MS.gene013126:CDS |
| GATGGTGATGGTGTTGCAGA+AGG | 0.612608 | 3.1:+79603195 | None:intergenic |
| TTGGTTCTGATTATAACACA+TGG | 0.617869 | 3.1:-79603646 | MS.gene013126:CDS |
| AGCTGAAGATGCACTTTGTG+TGG | 0.619201 | 3.1:+79603101 | None:intergenic |
| CTGCAACACCATCACCATCA+GGG | 0.628504 | 3.1:-79603191 | MS.gene013126:CDS |
| GAAGATGCACTTTGTGTGGA+AGG | 0.630949 | 3.1:+79603105 | None:intergenic |
| TGCACTTTGTGTGGAAGGAG+TGG | 0.632667 | 3.1:+79603110 | None:intergenic |
| CCGTTGGAGATAAATCAGGT+TGG | 0.633245 | 3.1:-79603673 | MS.gene013126:CDS |
| GGGAAAGGTTCACCTTCCGA+TGG | 0.642087 | 3.1:-79603171 | MS.gene013126:CDS |
| ACTTTGTGTGGAAGGAGTGG+TGG | 0.644852 | 3.1:+79603113 | None:intergenic |
| ACACCATCACCATCAGGGAA+AGG | 0.655836 | 3.1:-79603186 | MS.gene013126:CDS |
| TGGTTCTGATTATAACACAT+GGG | 0.658992 | 3.1:-79603645 | MS.gene013126:CDS |
| CTGATTTATCTCCAACGGTG+TGG | 0.663800 | 3.1:+79603678 | None:intergenic |
| TCAATTAGTACAGACAGTAG+TGG | 0.678569 | 3.1:-79603342 | MS.gene013126:CDS |
| CTATGGTGCTGGACACACAG+TGG | 0.764173 | 3.1:-79603409 | MS.gene013126:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATTAGATTTGTAAATTG+TGG | + | chr3.1:79603329-79603348 | None:intergenic | 15.0% |
| !! | GTGAAAAATAAAACAACAAT+TGG | + | chr3.1:79603721-79603740 | None:intergenic | 20.0% |
| !!! | TTTTATTTTTCACTGTTTCA+TGG | - | chr3.1:79603728-79603747 | MS.gene013126:CDS | 20.0% |
| ! | CTTCTTCTTAGCAATTAATA+TGG | - | chr3.1:79603061-79603080 | MS.gene013126:CDS | 25.0% |
| ! | GATCTTGATCAGTTATATAT+TGG | - | chr3.1:79603750-79603769 | MS.gene013126:CDS | 25.0% |
| !!! | TCTAATCCTTCTTTGATTTT+AGG | - | chr3.1:79603038-79603057 | MS.gene013126:CDS | 25.0% |
| AAGAAGCCTAAAATCAAAGA+AGG | + | chr3.1:79603047-79603066 | None:intergenic | 30.0% | |
| GAAACTTTCTGTTAAGGTTA+AGG | - | chr3.1:79603543-79603562 | MS.gene013126:intron | 30.0% | |
| !! | TGGTTCTGATTATAACACAT+GGG | - | chr3.1:79603139-79603158 | MS.gene013126:CDS | 30.0% |
| !! | TTGGTTCTGATTATAACACA+TGG | - | chr3.1:79603138-79603157 | MS.gene013126:CDS | 30.0% |
| ACTATGACAGTGTTCAACTA+TGG | - | chr3.1:79603358-79603377 | MS.gene013126:CDS | 35.0% | |
| GACTATAAATCATGCACAAC+TGG | - | chr3.1:79603415-79603434 | MS.gene013126:CDS | 35.0% | |
| TCAATTAGTACAGACAGTAG+TGG | - | chr3.1:79603442-79603461 | MS.gene013126:intron | 35.0% | |
| TGGCATGAAACTTTCTGTTA+AGG | - | chr3.1:79603537-79603556 | MS.gene013126:intron | 35.0% | |
| AGTGACAAAACATTTGCGGT+TGG | - | chr3.1:79603164-79603183 | MS.gene013126:CDS | 40.0% | |
| CAGCTTAATGAGAAGAAAGC+AGG | - | chr3.1:79603230-79603249 | MS.gene013126:CDS | 40.0% | |
| CCAACCTGATTTATCTCCAA+CGG | + | chr3.1:79603114-79603133 | None:intergenic | 40.0% | |
| CGTTGGAGATAAATCAGGTT+GGG | - | chr3.1:79603112-79603131 | MS.gene013126:CDS | 40.0% | |
| GGCTAGTGACAAAACATTTG+CGG | - | chr3.1:79603160-79603179 | MS.gene013126:CDS | 40.0% | |
| ! | TACTTCATATGTGCTGTTCC+TGG | - | chr3.1:79603502-79603521 | MS.gene013126:intron | 40.0% |
| !! | GATAAATCAGGTTGGGCAAT+TGG | - | chr3.1:79603119-79603138 | MS.gene013126:CDS | 40.0% |
| ACAGTGTTCAACTATGGTGC+TGG | - | chr3.1:79603364-79603383 | MS.gene013126:CDS | 45.0% | |
| AGCTGAAGATGCACTTTGTG+TGG | + | chr3.1:79603686-79603705 | None:intergenic | 45.0% | |
| CACACCGTTGGAGATAAATC+AGG | - | chr3.1:79603107-79603126 | MS.gene013126:CDS | 45.0% | |
| CCGTTGGAGATAAATCAGGT+TGG | - | chr3.1:79603111-79603130 | MS.gene013126:CDS | 45.0% | |
| CTGATTTATCTCCAACGGTG+TGG | + | chr3.1:79603109-79603128 | None:intergenic | 45.0% | |
| GAAGATGCACTTTGTGTGGA+AGG | + | chr3.1:79603682-79603701 | None:intergenic | 45.0% | |
| GTGTTCCTGCTTTCTTGAGA+GGG | + | chr3.1:79603480-79603499 | None:intergenic | 45.0% | |
| TGTGTTCCTGCTTTCTTGAG+AGG | + | chr3.1:79603481-79603500 | None:intergenic | 45.0% | |
| TTTCTTGAGAGGGATGGTTG+TGG | + | chr3.1:79603470-79603489 | None:intergenic | 45.0% | |
| ACACCATCACCATCAGGGAA+AGG | - | chr3.1:79603598-79603617 | MS.gene013126:intron | 50.0% | |
| ACCATCCCTCTCAAGAAAGC+AGG | - | chr3.1:79603472-79603491 | MS.gene013126:intron | 50.0% | |
| ACTTTGTGTGGAAGGAGTGG+TGG | + | chr3.1:79603674-79603693 | None:intergenic | 50.0% | |
| CTGCAACACCATCACCATCA+GGG | - | chr3.1:79603593-79603612 | MS.gene013126:intron | 50.0% | |
| GAAGGTGAACCTTTCCCTGA+TGG | + | chr3.1:79603610-79603629 | None:intergenic | 50.0% | |
| GTGTGGACAGTTGCAAGAGT+TGG | + | chr3.1:79603092-79603111 | None:intergenic | 50.0% | |
| TCCTGCTTTCTTGAGAGGGA+TGG | + | chr3.1:79603476-79603495 | None:intergenic | 50.0% | |
| TCTGCAACACCATCACCATC+AGG | - | chr3.1:79603592-79603611 | MS.gene013126:intron | 50.0% | |
| TGCACTTTGTGTGGAAGGAG+TGG | + | chr3.1:79603677-79603696 | None:intergenic | 50.0% | |
| ! | GAACCTTTCCCTGATGGTGA+TGG | + | chr3.1:79603604-79603623 | None:intergenic | 50.0% |
| !! | GATGGTGATGGTGTTGCAGA+AGG | + | chr3.1:79603592-79603611 | None:intergenic | 50.0% |
| !! | TTTGCGGTTGGTGACAGTCT+TGG | - | chr3.1:79603176-79603195 | MS.gene013126:CDS | 50.0% |
| ATGCCACCTGTGCAATGTCC+AGG | + | chr3.1:79603523-79603542 | None:intergenic | 55.0% | |
| CTTGCAACTGTCCACACCGT+TGG | - | chr3.1:79603095-79603114 | MS.gene013126:CDS | 55.0% | |
| GCTGTTCCTGGACATTGCAC+AGG | - | chr3.1:79603514-79603533 | MS.gene013126:intron | 55.0% | |
| GGGAAAGGTTCACCTTCCGA+TGG | - | chr3.1:79603613-79603632 | MS.gene013126:CDS | 55.0% | |
| GTTCCTGGACATTGCACAGG+TGG | - | chr3.1:79603517-79603536 | MS.gene013126:intron | 55.0% | |
| TTGTGTGGAAGGAGTGGTGG+TGG | + | chr3.1:79603671-79603690 | None:intergenic | 55.0% | |
| ! | CTATGGTGCTGGACACACAG+TGG | - | chr3.1:79603375-79603394 | MS.gene013126:CDS | 55.0% |
| !! | GAAGGAGTGGTGGTGGTGGA+GGG | + | chr3.1:79603664-79603683 | None:intergenic | 60.0% |
| !! | GTGTGGTGGTGGTTGCAGCA+GGG | + | chr3.1:79603642-79603661 | None:intergenic | 60.0% |
| !! | TGTGGAAGGAGTGGTGGTGG+TGG | + | chr3.1:79603668-79603687 | None:intergenic | 60.0% |
| !! | TGTGGTGGTGGTTGCAGCAG+GGG | + | chr3.1:79603641-79603660 | None:intergenic | 60.0% |
| !! | AGTGGTGGTGGTGGAGGGTG+TGG | + | chr3.1:79603659-79603678 | None:intergenic | 65.0% |
| !! | GGAAGGAGTGGTGGTGGTGG+AGG | + | chr3.1:79603665-79603684 | None:intergenic | 65.0% |
| !! | GGTGTGGTGGTGGTTGCAGC+AGG | + | chr3.1:79603643-79603662 | None:intergenic | 65.0% |
| !! | GGTTGCAGCAGGGGTGCCAT+CGG | + | chr3.1:79603632-79603651 | None:intergenic | 65.0% |
| !! | GCAGCAGGGGTGCCATCGGA+AGG | + | chr3.1:79603628-79603647 | None:intergenic | 70.0% |
| !! | GGTGGTGGAGGGTGTGGTGG+TGG | + | chr3.1:79603653-79603672 | None:intergenic | 70.0% |
| !! | GGTGGTGGTGGAGGGTGTGG+TGG | + | chr3.1:79603656-79603675 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 79603029 | 79603777 | 79603029 | ID=MS.gene013126 |
| chr3.1 | mRNA | 79603029 | 79603777 | 79603029 | ID=MS.gene013126.t1;Parent=MS.gene013126 |
| chr3.1 | exon | 79603609 | 79603777 | 79603609 | ID=MS.gene013126.t1.exon1;Parent=MS.gene013126.t1 |
| chr3.1 | CDS | 79603609 | 79603777 | 79603609 | ID=cds.MS.gene013126.t1;Parent=MS.gene013126.t1 |
| chr3.1 | exon | 79603029 | 79603438 | 79603029 | ID=MS.gene013126.t1.exon2;Parent=MS.gene013126.t1 |
| chr3.1 | CDS | 79603029 | 79603438 | 79603029 | ID=cds.MS.gene013126.t1;Parent=MS.gene013126.t1 |
| Gene Sequence |
| Protein sequence |
