Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014249.t1 | ADD09608.1 | 71.4 | 224 | 31 | 6 | 1 | 197 | 1 | 218 | 9.60E-44 | 186.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014249.t1 | P42763 | 46.7 | 90 | 31 | 4 | 1 | 86 | 1 | 77 | 6.2e-07 | 55.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014249.t1 | D3YBF7 | 71.4 | 224 | 31 | 6 | 1 | 197 | 1 | 218 | 6.9e-44 | 186.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene014249 | MS.gene68191 | PPI |
| MS.gene68785 | MS.gene014249 | PPI |
| MS.gene063134 | MS.gene014249 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014249.t1 | MTR_3g117290 | 72.398 | 221 | 25 | 7 | 1 | 197 | 1 | 209 | 2.74e-71 | 215 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014249.t1 | AT1G76180 | 44.000 | 200 | 76 | 6 | 1 | 184 | 1 | 180 | 1.39e-23 | 92.8 |
| MS.gene014249.t1 | AT1G76180 | 44.000 | 200 | 76 | 6 | 1 | 184 | 1 | 180 | 1.39e-23 | 92.8 |
| MS.gene014249.t1 | AT1G20440 | 42.233 | 206 | 81 | 11 | 1 | 176 | 1 | 198 | 1.42e-19 | 84.0 |
| MS.gene014249.t1 | AT1G20450 | 36.321 | 212 | 87 | 10 | 1 | 175 | 1 | 201 | 3.06e-15 | 72.4 |
| MS.gene014249.t1 | AT1G20450 | 54.667 | 75 | 28 | 2 | 110 | 184 | 185 | 253 | 1.26e-12 | 65.1 |
| MS.gene014249.t1 | AT1G20450 | 36.019 | 211 | 88 | 10 | 1 | 175 | 1 | 200 | 1.92e-14 | 70.1 |
| MS.gene014249.t1 | AT1G20450 | 55.263 | 76 | 26 | 3 | 110 | 184 | 184 | 252 | 1.29e-12 | 65.1 |
Find 35 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAGAGAAGAAAGGTATTT+TGG | 0.202968 | 3.3:+95244325 | MS.gene014249:CDS |
| TCATGTTCAGTAGCAGTCTT+AGG | 0.274530 | 3.3:-95244380 | None:intergenic |
| AGGTTTCCTGGACAAAATTA+AGG | 0.300059 | 3.3:+95244190 | MS.gene014249:CDS |
| AAAATTAAGGAGAAGCTTCC+AGG | 0.351108 | 3.3:+95244203 | MS.gene014249:CDS |
| TGAAGAGAAGAAAGGTTTCC+TGG | 0.355767 | 3.3:+95244178 | MS.gene014249:CDS |
| TCTTCTACTTTGGTCTCTGA+TGG | 0.368786 | 3.3:-95243767 | None:intergenic |
| GAGAAAGTTGATGTTGTTGA+TGG | 0.398275 | 3.3:+95244140 | MS.gene014249:CDS |
| TTTGATCTCTGTTTCAGAGT+TGG | 0.421884 | 3.3:-95243630 | None:intergenic |
| GTCTCAGCAGATGGCACAGC+AGG | 0.446448 | 3.3:-95244275 | None:intergenic |
| ACAGCAGGAGCAACAACAAC+AGG | 0.468581 | 3.3:-95244260 | None:intergenic |
| AAAGAAACACACCAGCCTCT+TGG | 0.485431 | 3.3:+95243805 | MS.gene014249:CDS |
| CATGTTCAGTAGCAGTCTTA+GGG | 0.495120 | 3.3:-95244379 | None:intergenic |
| GTTGTTGTTGTCTCAGCAGA+TGG | 0.500792 | 3.3:-95244284 | None:intergenic |
| CTCTGTTTCAGAGTTGGTTG+CGG | 0.507900 | 3.3:-95243624 | None:intergenic |
| ACAACATCAACTTTCTCTAC+TGG | 0.509084 | 3.3:-95244134 | None:intergenic |
| CTTCTCTTCAGTGCTTGCTG+TGG | 0.512857 | 3.3:-95244165 | None:intergenic |
| GTTGCAACAGCATCCTCTTG+AGG | 0.534346 | 3.3:-95243710 | None:intergenic |
| GTGAAGTTTCTCCAAGAGGC+TGG | 0.546170 | 3.3:-95243816 | None:intergenic |
| GCAAGCACTGAAGAGAAGAA+AGG | 0.548209 | 3.3:+95244170 | MS.gene014249:CDS |
| CATGATCAAGGAGAGAAGAA+AGG | 0.558513 | 3.3:+95244317 | MS.gene014249:CDS |
| TAATCAATTCATCATGGCTG+AGG | 0.573271 | 3.3:+95243574 | None:intergenic |
| ACAACAGCTAGTCATGATCA+AGG | 0.576125 | 3.3:+95244305 | MS.gene014249:CDS |
| CTGAAACAGAGATCAAAGAC+AGG | 0.576169 | 3.3:+95243636 | MS.gene014249:CDS |
| TGTTTCAGAGTTGGTTGCGG+TGG | 0.586483 | 3.3:-95243621 | None:intergenic |
| GGAGCAACAACAACAGGTGG+TGG | 0.591480 | 3.3:-95244254 | None:intergenic |
| AAGATCAAAGAGAAGATACC+TGG | 0.609148 | 3.3:+95244350 | MS.gene014249:CDS |
| AGAACATATTAAGCCTCAAG+AGG | 0.614539 | 3.3:+95243697 | MS.gene014249:CDS |
| GCAGTCTTAGGGTGATAACC+AGG | 0.619784 | 3.3:-95244368 | None:intergenic |
| ATCGGTGAAGTTTCTCCAAG+AGG | 0.645229 | 3.3:-95243820 | None:intergenic |
| TGAAACAGAGATCAAAGACA+GGG | 0.654650 | 3.3:+95243637 | MS.gene014249:CDS |
| GCAGGAGCAACAACAACAGG+TGG | 0.666368 | 3.3:-95244257 | None:intergenic |
| AGCTTGAAGAGCTATCAGAT+CGG | 0.690728 | 3.3:-95243838 | None:intergenic |
| AGACAACAAAGATCATCACA+AGG | 0.715357 | 3.3:+95244403 | MS.gene014249:CDS |
| GGAGAATCAGAACAAGTACG+AGG | 0.738041 | 3.3:+95243595 | MS.gene014249:CDS |
| GAAACAGAGATCAAAGACAG+GGG | 0.744878 | 3.3:+95243638 | MS.gene014249:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAAGAAGAAAGAAAAGA+AGG | + | chr3.3:95244088-95244107 | MS.gene014249:intron | 25.0% |
| ! | AAAGTAGAAGAAAAAGAAGA+AGG | + | chr3.3:95243779-95243798 | MS.gene014249:CDS | 25.0% |
| !! | TTCTTCTTTTTCTTCTACTT+TGG | - | chr3.3:95243780-95243799 | None:intergenic | 25.0% |
| ! | TTTTCTAGGTGGTAAGAAAA+AGG | + | chr3.3:95243670-95243689 | MS.gene014249:CDS | 30.0% |
| AAAATTAAGGAGAAGCTTCC+AGG | + | chr3.3:95244203-95244222 | MS.gene014249:CDS | 35.0% | |
| AAGATCAAAGAGAAGATACC+TGG | + | chr3.3:95244350-95244369 | MS.gene014249:CDS | 35.0% | |
| ACAACATCAACTTTCTCTAC+TGG | - | chr3.3:95244137-95244156 | None:intergenic | 35.0% | |
| AGAACATATTAAGCCTCAAG+AGG | + | chr3.3:95243697-95243716 | MS.gene014249:CDS | 35.0% | |
| AGACAACAAAGATCATCACA+AGG | + | chr3.3:95244403-95244422 | MS.gene014249:CDS | 35.0% | |
| AGGTTTCCTGGACAAAATTA+AGG | + | chr3.3:95244190-95244209 | MS.gene014249:CDS | 35.0% | |
| GAAAGAAAAGAAGGAGAAGA+AGG | + | chr3.3:95244097-95244116 | MS.gene014249:intron | 35.0% | |
| TGAAACAGAGATCAAAGACA+GGG | + | chr3.3:95243637-95243656 | MS.gene014249:CDS | 35.0% | |
| TGTATTGAATACGTGTCAAG+TGG | + | chr3.3:95243924-95243943 | MS.gene014249:intron | 35.0% | |
| TTTGATCTCTGTTTCAGAGT+TGG | - | chr3.3:95243633-95243652 | None:intergenic | 35.0% | |
| !! | GAGAAAGTTGATGTTGTTGA+TGG | + | chr3.3:95244140-95244159 | MS.gene014249:CDS | 35.0% |
| !! | TGCAACTGATTTTAGTCACA+AGG | + | chr3.3:95243727-95243746 | MS.gene014249:CDS | 35.0% |
| !!! | AGGAGAGAAGAAAGGTATTT+TGG | + | chr3.3:95244325-95244344 | MS.gene014249:CDS | 35.0% |
| !!! | AGGGGTGTTTTTGATTTTCT+AGG | + | chr3.3:95243656-95243675 | MS.gene014249:CDS | 35.0% |
| !!! | GGTGTTTTTGATTTTCTAGG+TGG | + | chr3.3:95243659-95243678 | MS.gene014249:CDS | 35.0% |
| ACAACAGCTAGTCATGATCA+AGG | + | chr3.3:95244305-95244324 | MS.gene014249:CDS | 40.0% | |
| AGAAAAGAAGGAGAAGAAGG+AGG | + | chr3.3:95244100-95244119 | MS.gene014249:intron | 40.0% | |
| AGCTTGAAGAGCTATCAGAT+CGG | - | chr3.3:95243841-95243860 | None:intergenic | 40.0% | |
| CATGATCAAGGAGAGAAGAA+AGG | + | chr3.3:95244317-95244336 | MS.gene014249:CDS | 40.0% | |
| CATGTTCAGTAGCAGTCTTA+GGG | - | chr3.3:95244382-95244401 | None:intergenic | 40.0% | |
| CTGAAACAGAGATCAAAGAC+AGG | + | chr3.3:95243636-95243655 | MS.gene014249:CDS | 40.0% | |
| GAAACAGAGATCAAAGACAG+GGG | + | chr3.3:95243638-95243657 | MS.gene014249:CDS | 40.0% | |
| TATGCATGTATAGTCGAGTG+AGG | + | chr3.3:95244040-95244059 | MS.gene014249:intron | 40.0% | |
| TCATGTTCAGTAGCAGTCTT+AGG | - | chr3.3:95244383-95244402 | None:intergenic | 40.0% | |
| TCTTCTACTTTGGTCTCTGA+TGG | - | chr3.3:95243770-95243789 | None:intergenic | 40.0% | |
| TGAAGAGAAGAAAGGTTTCC+TGG | + | chr3.3:95244178-95244197 | MS.gene014249:CDS | 40.0% | |
| ! | GCTTCTCCTTAATTTTGTCC+AGG | - | chr3.3:95244199-95244218 | None:intergenic | 40.0% |
| AAAGAAACACACCAGCCTCT+TGG | + | chr3.3:95243805-95243824 | MS.gene014249:CDS | 45.0% | |
| ATCGGTGAAGTTTCTCCAAG+AGG | - | chr3.3:95243823-95243842 | None:intergenic | 45.0% | |
| GACTGAACGTAGTGTCAACA+AGG | - | chr3.3:95243958-95243977 | None:intergenic | 45.0% | |
| GGAGAATCAGAACAAGTACG+AGG | + | chr3.3:95243595-95243614 | MS.gene014249:CDS | 45.0% | |
| ! | CTCTGTTTCAGAGTTGGTTG+CGG | - | chr3.3:95243627-95243646 | None:intergenic | 45.0% |
| ! | GTTGTTGTTGTCTCAGCAGA+TGG | - | chr3.3:95244287-95244306 | None:intergenic | 45.0% |
| ! | TCGTCAGTTTTCTTGTGTCC+TGG | - | chr3.3:95244224-95244243 | None:intergenic | 45.0% |
| !! | GCAAGCACTGAAGAGAAGAA+AGG | + | chr3.3:95244170-95244189 | MS.gene014249:CDS | 45.0% |
| ACAGCAGGAGCAACAACAAC+AGG | - | chr3.3:95244263-95244282 | None:intergenic | 50.0% | |
| ATACGTGTCAAGTGGCAGAG+TGG | + | chr3.3:95243932-95243951 | MS.gene014249:intron | 50.0% | |
| GCAGTCTTAGGGTGATAACC+AGG | - | chr3.3:95244371-95244390 | None:intergenic | 50.0% | |
| GCATGTATAGTCGAGTGAGG+AGG | + | chr3.3:95244043-95244062 | MS.gene014249:intron | 50.0% | |
| GTGAAGTTTCTCCAAGAGGC+TGG | - | chr3.3:95243819-95243838 | None:intergenic | 50.0% | |
| GTTGCAACAGCATCCTCTTG+AGG | - | chr3.3:95243713-95243732 | None:intergenic | 50.0% | |
| TCGAGTGAGGAGGAAGTTGA+TGG | + | chr3.3:95244053-95244072 | MS.gene014249:intron | 50.0% | |
| ! | CTTCTCTTCAGTGCTTGCTG+TGG | - | chr3.3:95244168-95244187 | None:intergenic | 50.0% |
| ! | TGTTTCAGAGTTGGTTGCGG+TGG | - | chr3.3:95243624-95243643 | None:intergenic | 50.0% |
| !! | AGGAAGTTGATGGAGAGAGG+AGG | + | chr3.3:95244063-95244082 | MS.gene014249:intron | 50.0% |
| !! | AGGAGGAAGTTGATGGAGAG+AGG | + | chr3.3:95244060-95244079 | MS.gene014249:intron | 50.0% |
| GCAGGAGCAACAACAACAGG+TGG | - | chr3.3:95244260-95244279 | None:intergenic | 55.0% | |
| GGAGCAACAACAACAGGTGG+TGG | - | chr3.3:95244257-95244276 | None:intergenic | 55.0% | |
| GTCTCAGCAGATGGCACAGC+AGG | - | chr3.3:95244278-95244297 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 95243587 | 95244445 | 95243587 | ID=MS.gene014249 |
| chr3.3 | mRNA | 95243587 | 95244445 | 95243587 | ID=MS.gene014249.t1;Parent=MS.gene014249 |
| chr3.3 | exon | 95243587 | 95243866 | 95243587 | ID=MS.gene014249.t1.exon1;Parent=MS.gene014249.t1 |
| chr3.3 | CDS | 95243587 | 95243866 | 95243587 | ID=cds.MS.gene014249.t1;Parent=MS.gene014249.t1 |
| chr3.3 | exon | 95244132 | 95244445 | 95244132 | ID=MS.gene014249.t1.exon2;Parent=MS.gene014249.t1 |
| chr3.3 | CDS | 95244132 | 95244445 | 95244132 | ID=cds.MS.gene014249.t1;Parent=MS.gene014249.t1 |
| Gene Sequence |
| Protein sequence |
