Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68785.t1 | ADD09608.1 | 81.2 | 223 | 36 | 4 | 1 | 222 | 1 | 218 | 2.00E-37 | 166 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68785.t1 | D3YBF7 | 81.2 | 223 | 36 | 4 | 1 | 222 | 1 | 218 | 1.4e-37 | 166.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059949 | MS.gene68785 | 0.821614 | 3.68E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene063134 | MS.gene68785 | PPI |
| MS.gene68785 | MS.gene063134 | PPI |
| MS.gene68785 | MS.gene68191 | PPI |
| MS.gene68785 | MS.gene014249 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68785.t1 | MTR_3g117290 | 83.036 | 224 | 21 | 7 | 1 | 222 | 1 | 209 | 1.48e-77 | 232 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68785.t1 | AT1G76180 | 46.948 | 213 | 76 | 6 | 1 | 209 | 1 | 180 | 3.67e-22 | 89.7 |
| MS.gene68785.t1 | AT1G76180 | 46.948 | 213 | 76 | 6 | 1 | 209 | 1 | 180 | 3.67e-22 | 89.7 |
| MS.gene68785.t1 | AT1G20450 | 39.015 | 264 | 95 | 11 | 1 | 209 | 1 | 253 | 9.26e-21 | 87.8 |
| MS.gene68785.t1 | AT1G20440 | 40.000 | 255 | 93 | 11 | 1 | 201 | 1 | 249 | 5.28e-20 | 85.9 |
| MS.gene68785.t1 | AT1G20450 | 39.163 | 263 | 95 | 11 | 1 | 209 | 1 | 252 | 6.34e-20 | 85.5 |
Find 42 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAGAGAAGAAAGGTATTT+TGG | 0.202968 | 3.2:+92613465 | MS.gene68785:CDS |
| TCATGTTCAGTAGCAGTCTT+AGG | 0.274530 | 3.2:-92613520 | None:intergenic |
| AGGTTTCCTGGACAAAATTA+AGG | 0.300059 | 3.2:+92613330 | MS.gene68785:CDS |
| AAAATTAAGGAGAAGCTTCC+AGG | 0.351108 | 3.2:+92613343 | MS.gene68785:CDS |
| TGAAGAGAAGAAAGGTTTCC+TGG | 0.355767 | 3.2:+92613318 | MS.gene68785:CDS |
| TCTTCTACTTTGGTCTCTGA+TGG | 0.368786 | 3.2:-92612908 | None:intergenic |
| AAAGAAGAAGAAAGAAAAGA+AGG | 0.380764 | 3.2:+92613228 | MS.gene68785:CDS |
| GAAAGAAAAGAAGGAGAAGA+AGG | 0.381297 | 3.2:+92613237 | MS.gene68785:CDS |
| TCGAGTGAGGAGGAAGTTGA+TGG | 0.382576 | 3.2:+92613193 | MS.gene68785:CDS |
| GAGAAAGTTGATGTTGTTGA+TGG | 0.398275 | 3.2:+92613280 | MS.gene68785:CDS |
| TTTGATCTCTGTTTCAGAGT+TGG | 0.421884 | 3.2:-92612771 | None:intergenic |
| GTCTCAGCAGATGGCACAGC+AGG | 0.446448 | 3.2:-92613415 | None:intergenic |
| AAGATCAAAGAGAAGATTCC+TGG | 0.454599 | 3.2:+92613490 | MS.gene68785:CDS |
| ACAGCAGGAGCAACAACAAC+AGG | 0.468581 | 3.2:-92613400 | None:intergenic |
| AAAGAAACACACCAGCCTCT+TGG | 0.485431 | 3.2:+92612946 | MS.gene68785:CDS |
| AGGAGGAAGTTGATGGAGAG+AGG | 0.487866 | 3.2:+92613200 | MS.gene68785:CDS |
| CATGTTCAGTAGCAGTCTTA+GGG | 0.495120 | 3.2:-92613519 | None:intergenic |
| GTTGTTGTTGTCTCAGCAGA+TGG | 0.500792 | 3.2:-92613424 | None:intergenic |
| CTCTGTTTCAGAGTTGGTTG+CGG | 0.507900 | 3.2:-92612765 | None:intergenic |
| ACAACATCAACTTTCTCTAC+TGG | 0.509084 | 3.2:-92613274 | None:intergenic |
| GTTGCAACAGCATCCTCTTG+AGG | 0.534346 | 3.2:-92612851 | None:intergenic |
| GTGAAGTTTCTCCAAGAGGC+TGG | 0.546170 | 3.2:-92612957 | None:intergenic |
| GCAAGCACTGAAGAGAAGAA+AGG | 0.548209 | 3.2:+92613310 | MS.gene68785:CDS |
| CATGATCAAGGAGAGAAGAA+AGG | 0.558513 | 3.2:+92613457 | MS.gene68785:CDS |
| TAATCAATTCATCATGGCTG+AGG | 0.573271 | 3.2:+92612715 | None:intergenic |
| ACAACAGCTAGTCATGATCA+AGG | 0.576125 | 3.2:+92613445 | MS.gene68785:CDS |
| CTGAAACAGAGATCAAAGAC+AGG | 0.576169 | 3.2:+92612777 | MS.gene68785:CDS |
| AGAAAAGAAGGAGAAGAAGG+AGG | 0.577972 | 3.2:+92613240 | MS.gene68785:CDS |
| TGTTTCAGAGTTGGTTGCGG+TGG | 0.586483 | 3.2:-92612762 | None:intergenic |
| AGGAAGTTGATGGAGAGAGG+AGG | 0.587456 | 3.2:+92613203 | MS.gene68785:CDS |
| GGAGCAACAACAACAGGTGG+TGG | 0.591480 | 3.2:-92613394 | None:intergenic |
| GCAGTCTTAGGGTGATAACC+AGG | 0.611315 | 3.2:-92613508 | None:intergenic |
| AGAACATATTAAGCCTCAAG+AGG | 0.614539 | 3.2:+92612838 | MS.gene68785:CDS |
| TATGCATGTATAGTCGAGTG+AGG | 0.638826 | 3.2:+92613180 | MS.gene68785:intron |
| GCATGTATAGTCGAGTGAGG+AGG | 0.641425 | 3.2:+92613183 | MS.gene68785:intron |
| ATCGGTGAAGTTTCTCCAAG+AGG | 0.645229 | 3.2:-92612961 | None:intergenic |
| TGAAACAGAGATCAAAGACA+GGG | 0.654650 | 3.2:+92612778 | MS.gene68785:CDS |
| GCAGGAGCAACAACAACAGG+TGG | 0.666368 | 3.2:-92613397 | None:intergenic |
| AGCTTGAAGAGCTATCAGAT+CGG | 0.690728 | 3.2:-92612979 | None:intergenic |
| AGACAACAAAGATCATCACA+AGG | 0.715357 | 3.2:+92613543 | MS.gene68785:CDS |
| GGAGAATCAGAACAAGTACG+AGG | 0.738041 | 3.2:+92612736 | MS.gene68785:CDS |
| GAAACAGAGATCAAAGACAG+GGG | 0.744878 | 3.2:+92612779 | MS.gene68785:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAAGAAGAAAGAAAAGA+AGG | + | chr3.2:92613228-92613247 | MS.gene68785:CDS | 25.0% |
| ! | AAAGTAGAAGAAAAAGAAGA+AGG | + | chr3.2:92612920-92612939 | MS.gene68785:CDS | 25.0% |
| !! | TTCTTCTTTTTCTTCTACTT+TGG | - | chr3.2:92612921-92612940 | None:intergenic | 25.0% |
| TGTATTGAATATGTGTCAAG+TGG | + | chr3.2:92613060-92613079 | MS.gene68785:intron | 30.0% | |
| ! | TTTTCTAGGTGGTAAGAAAA+AGG | + | chr3.2:92612811-92612830 | MS.gene68785:CDS | 30.0% |
| AAAATTAAGGAGAAGCTTCC+AGG | + | chr3.2:92613343-92613362 | MS.gene68785:CDS | 35.0% | |
| AAGATCAAAGAGAAGATTCC+TGG | + | chr3.2:92613490-92613509 | MS.gene68785:CDS | 35.0% | |
| ACAACATCAACTTTCTCTAC+TGG | - | chr3.2:92613277-92613296 | None:intergenic | 35.0% | |
| AGAACATATTAAGCCTCAAG+AGG | + | chr3.2:92612838-92612857 | MS.gene68785:CDS | 35.0% | |
| AGACAACAAAGATCATCACA+AGG | + | chr3.2:92613543-92613562 | MS.gene68785:CDS | 35.0% | |
| AGGTTTCCTGGACAAAATTA+AGG | + | chr3.2:92613330-92613349 | MS.gene68785:CDS | 35.0% | |
| GAAAGAAAAGAAGGAGAAGA+AGG | + | chr3.2:92613237-92613256 | MS.gene68785:CDS | 35.0% | |
| TGAAACAGAGATCAAAGACA+GGG | + | chr3.2:92612778-92612797 | MS.gene68785:CDS | 35.0% | |
| TTTGATCTCTGTTTCAGAGT+TGG | - | chr3.2:92612774-92612793 | None:intergenic | 35.0% | |
| !! | GAGAAAGTTGATGTTGTTGA+TGG | + | chr3.2:92613280-92613299 | MS.gene68785:CDS | 35.0% |
| !! | TGCAACTGATTTTAGTCACA+AGG | + | chr3.2:92612868-92612887 | MS.gene68785:CDS | 35.0% |
| !!! | AGGAGAGAAGAAAGGTATTT+TGG | + | chr3.2:92613465-92613484 | MS.gene68785:CDS | 35.0% |
| !!! | AGGGGTGTTTTTGATTTTCT+AGG | + | chr3.2:92612797-92612816 | MS.gene68785:CDS | 35.0% |
| !!! | GGTGTTTTTGATTTTCTAGG+TGG | + | chr3.2:92612800-92612819 | MS.gene68785:CDS | 35.0% |
| ACAACAGCTAGTCATGATCA+AGG | + | chr3.2:92613445-92613464 | MS.gene68785:CDS | 40.0% | |
| AGAAAAGAAGGAGAAGAAGG+AGG | + | chr3.2:92613240-92613259 | MS.gene68785:CDS | 40.0% | |
| AGCTTGAAGAGCTATCAGAT+CGG | - | chr3.2:92612982-92613001 | None:intergenic | 40.0% | |
| CATGATCAAGGAGAGAAGAA+AGG | + | chr3.2:92613457-92613476 | MS.gene68785:CDS | 40.0% | |
| CATGTTCAGTAGCAGTCTTA+GGG | - | chr3.2:92613522-92613541 | None:intergenic | 40.0% | |
| CTGAAACAGAGATCAAAGAC+AGG | + | chr3.2:92612777-92612796 | MS.gene68785:CDS | 40.0% | |
| GAAACAGAGATCAAAGACAG+GGG | + | chr3.2:92612779-92612798 | MS.gene68785:CDS | 40.0% | |
| GATTGAACGTAGTGTCAACA+AGG | - | chr3.2:92613094-92613113 | None:intergenic | 40.0% | |
| TATGCATGTATAGTCGAGTG+AGG | + | chr3.2:92613180-92613199 | MS.gene68785:intron | 40.0% | |
| TCATGTTCAGTAGCAGTCTT+AGG | - | chr3.2:92613523-92613542 | None:intergenic | 40.0% | |
| TCTTCTACTTTGGTCTCTGA+TGG | - | chr3.2:92612911-92612930 | None:intergenic | 40.0% | |
| TGAAGAGAAGAAAGGTTTCC+TGG | + | chr3.2:92613318-92613337 | MS.gene68785:CDS | 40.0% | |
| ! | GCTTCTCCTTAATTTTGTCC+AGG | - | chr3.2:92613339-92613358 | None:intergenic | 40.0% |
| AAAGAAACACACCAGCCTCT+TGG | + | chr3.2:92612946-92612965 | MS.gene68785:CDS | 45.0% | |
| ATCGGTGAAGTTTCTCCAAG+AGG | - | chr3.2:92612964-92612983 | None:intergenic | 45.0% | |
| GGAGAATCAGAACAAGTACG+AGG | + | chr3.2:92612736-92612755 | MS.gene68785:CDS | 45.0% | |
| ! | CTCTGTTTCAGAGTTGGTTG+CGG | - | chr3.2:92612768-92612787 | None:intergenic | 45.0% |
| ! | GTTGTTGTTGTCTCAGCAGA+TGG | - | chr3.2:92613427-92613446 | None:intergenic | 45.0% |
| ! | TCGTCAGTTTTCTTGTGTCC+TGG | - | chr3.2:92613364-92613383 | None:intergenic | 45.0% |
| !! | GCAAGCACTGAAGAGAAGAA+AGG | + | chr3.2:92613310-92613329 | MS.gene68785:CDS | 45.0% |
| ACAGCAGGAGCAACAACAAC+AGG | - | chr3.2:92613403-92613422 | None:intergenic | 50.0% | |
| GCAGTCTTAGGGTGATAACC+AGG | - | chr3.2:92613511-92613530 | None:intergenic | 50.0% | |
| GCATGTATAGTCGAGTGAGG+AGG | + | chr3.2:92613183-92613202 | MS.gene68785:intron | 50.0% | |
| GTGAAGTTTCTCCAAGAGGC+TGG | - | chr3.2:92612960-92612979 | None:intergenic | 50.0% | |
| GTTGCAACAGCATCCTCTTG+AGG | - | chr3.2:92612854-92612873 | None:intergenic | 50.0% | |
| TCGAGTGAGGAGGAAGTTGA+TGG | + | chr3.2:92613193-92613212 | MS.gene68785:CDS | 50.0% | |
| ! | TGTTTCAGAGTTGGTTGCGG+TGG | - | chr3.2:92612765-92612784 | None:intergenic | 50.0% |
| !! | AGGAAGTTGATGGAGAGAGG+AGG | + | chr3.2:92613203-92613222 | MS.gene68785:CDS | 50.0% |
| !! | AGGAGGAAGTTGATGGAGAG+AGG | + | chr3.2:92613200-92613219 | MS.gene68785:CDS | 50.0% |
| GCAGGAGCAACAACAACAGG+TGG | - | chr3.2:92613400-92613419 | None:intergenic | 55.0% | |
| GGAGCAACAACAACAGGTGG+TGG | - | chr3.2:92613397-92613416 | None:intergenic | 55.0% | |
| GTCTCAGCAGATGGCACAGC+AGG | - | chr3.2:92613418-92613437 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 92612728 | 92613585 | 92612728 | ID=MS.gene68785 |
| chr3.2 | mRNA | 92612728 | 92613585 | 92612728 | ID=MS.gene68785.t1;Parent=MS.gene68785 |
| chr3.2 | exon | 92612728 | 92613003 | 92612728 | ID=MS.gene68785.t1.exon1;Parent=MS.gene68785.t1 |
| chr3.2 | CDS | 92612728 | 92613003 | 92612728 | ID=cds.MS.gene68785.t1;Parent=MS.gene68785.t1 |
| chr3.2 | exon | 92613193 | 92613585 | 92613193 | ID=MS.gene68785.t1.exon2;Parent=MS.gene68785.t1 |
| chr3.2 | CDS | 92613193 | 92613585 | 92613193 | ID=cds.MS.gene68785.t1;Parent=MS.gene68785.t1 |
| Gene Sequence |
| Protein sequence |
