AlfalfaGEDB | MS.gene063134

M. sativa cultivar XinJiangDaYe / MS.gene063134

KEGG: -
GO: GO:0001101 GO:0008150 GO:0009415 GO:0009628 GO:0010035 GO:0042221 GO:0050896 GO:1901700
PFAM: PF00257.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063134.t1	ADD09608.1	71.4	224	31	6	1	197	1	218	9.60E-44	186.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063134.t1	P42763	46.7	90	31	4	1	86	1	77	6.2e-07	55.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063134.t1	D3YBF7	71.4	224	31	6	1	197	1	218	6.9e-44	186.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055150	MS.gene063134	0.804113	2.52E-49	-1.69E-46
MS.gene056693	MS.gene063134	0.801091	1.06E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene063134	MS.gene68785	PPI
MS.gene68785	MS.gene063134	PPI
MS.gene063134	MS.gene68191	PPI
MS.gene063134	MS.gene014249	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063134.t1	MTR_3g117290	72.398	221	25	7	1	197	1	209	2.74e-71	215

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063134.t1	AT1G76180	44.000	200	76	6	1	184	1	180	1.39e-23	92.8
MS.gene063134.t1	AT1G76180	44.000	200	76	6	1	184	1	180	1.39e-23	92.8
MS.gene063134.t1	AT1G20440	42.233	206	81	11	1	176	1	198	1.42e-19	84.0
MS.gene063134.t1	AT1G20450	36.321	212	87	10	1	175	1	201	3.06e-15	72.4
MS.gene063134.t1	AT1G20450	54.667	75	28	2	110	184	185	253	1.26e-12	65.1
MS.gene063134.t1	AT1G20450	36.019	211	88	10	1	175	1	200	1.92e-14	70.1
MS.gene063134.t1	AT1G20450	55.263	76	26	3	110	184	184	252	1.29e-12	65.1

Find 34 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGAGAGAAGAAAGGTATTT+TGG	0.202968	3.4:+99685681	MS.gene063134:CDS
TCATGTTCAGTAGCAGTCTT+AGG	0.274530	3.4:-99685736	None:intergenic
AGGTTTCCTGGACAAAATTA+AGG	0.300059	3.4:+99685546	MS.gene063134:CDS
AAAATTAAGGAGAAGCTTCC+AGG	0.351108	3.4:+99685559	MS.gene063134:CDS
TGAAGAGAAGAAAGGTTTCC+TGG	0.355767	3.4:+99685534	MS.gene063134:CDS
TCTTCTACTTTGGTCTCTGA+TGG	0.368786	3.4:-99685127	None:intergenic
GAGAAAGTTGATGTTGTTGA+TGG	0.398275	3.4:+99685496	MS.gene063134:CDS
TTTGATCTCTGTTTCAGAGT+TGG	0.421884	3.4:-99684990	None:intergenic
GTCTCAGCAGATGGCACAGC+AGG	0.446448	3.4:-99685631	None:intergenic
ACAGCAGGAGCAACAACAAC+AGG	0.468581	3.4:-99685616	None:intergenic
AAAGAAACACACCAGCCTCT+TGG	0.485431	3.4:+99685165	MS.gene063134:CDS
CATGTTCAGTAGCAGTCTTA+GGG	0.495120	3.4:-99685735	None:intergenic
GTTGTTGTTGTCTCAGCAGA+TGG	0.500792	3.4:-99685640	None:intergenic
CTCTGTTTCAGAGTTGGTTG+CGG	0.507900	3.4:-99684984	None:intergenic
ACAACATCAACTTTCTCTAC+TGG	0.509084	3.4:-99685490	None:intergenic
GTTGCAACAGCATCCTCTTG+AGG	0.534346	3.4:-99685070	None:intergenic
GTGAAGTTTCTCCAAGAGGC+TGG	0.546170	3.4:-99685176	None:intergenic
GCAAGCACTGAAGAGAAGAA+AGG	0.548209	3.4:+99685526	MS.gene063134:CDS
CATGATCAAGGAGAGAAGAA+AGG	0.558513	3.4:+99685673	MS.gene063134:CDS
TAATCAATTCATCATGGCTG+AGG	0.573271	3.4:+99684934	None:intergenic
ACAACAGCTAGTCATGATCA+AGG	0.576125	3.4:+99685661	MS.gene063134:CDS
CTGAAACAGAGATCAAAGAC+AGG	0.576169	3.4:+99684996	MS.gene063134:CDS
TGTTTCAGAGTTGGTTGCGG+TGG	0.586483	3.4:-99684981	None:intergenic
GGAGCAACAACAACAGGTGG+TGG	0.591480	3.4:-99685610	None:intergenic
AAGATCAAAGAGAAGATACC+TGG	0.609148	3.4:+99685706	MS.gene063134:CDS
AGAACATATTAAGCCTCAAG+AGG	0.614539	3.4:+99685057	MS.gene063134:CDS
GCAGTCTTAGGGTGATAACC+AGG	0.619784	3.4:-99685724	None:intergenic
ATCGGTGAAGTTTCTCCAAG+AGG	0.645229	3.4:-99685180	None:intergenic
TGAAACAGAGATCAAAGACA+GGG	0.654650	3.4:+99684997	MS.gene063134:CDS
GCAGGAGCAACAACAACAGG+TGG	0.666368	3.4:-99685613	None:intergenic
AGCTTGAAGAGCTATCAGAT+CGG	0.690728	3.4:-99685198	None:intergenic
AGACAACAAAGATCATCACA+AGG	0.715357	3.4:+99685759	MS.gene063134:CDS
GGAGAATCAGAACAAGTACG+AGG	0.738041	3.4:+99684955	MS.gene063134:CDS
GAAACAGAGATCAAAGACAG+GGG	0.744878	3.4:+99684998	MS.gene063134:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAGAAGAAGAAAGAAAAGA+AGG	+	chr3.4:99685444-99685463	MS.gene063134:intron	25.0%
!	AAAGTAGAAGAAAAAGAAGA+AGG	+	chr3.4:99685139-99685158	MS.gene063134:CDS	25.0%
!!	TTCTTCTTTTTCTTCTACTT+TGG	-	chr3.4:99685140-99685159	None:intergenic	25.0%
!	TTTTCTAGGTGGTAAGAAAA+AGG	+	chr3.4:99685030-99685049	MS.gene063134:CDS	30.0%
	AAAATTAAGGAGAAGCTTCC+AGG	+	chr3.4:99685559-99685578	MS.gene063134:CDS	35.0%
	AAGATCAAAGAGAAGATACC+TGG	+	chr3.4:99685706-99685725	MS.gene063134:CDS	35.0%
	ACAACATCAACTTTCTCTAC+TGG	-	chr3.4:99685493-99685512	None:intergenic	35.0%
	AGAACATATTAAGCCTCAAG+AGG	+	chr3.4:99685057-99685076	MS.gene063134:CDS	35.0%
	AGACAACAAAGATCATCACA+AGG	+	chr3.4:99685759-99685778	MS.gene063134:CDS	35.0%
	AGGTTTCCTGGACAAAATTA+AGG	+	chr3.4:99685546-99685565	MS.gene063134:CDS	35.0%
	GAAAGAAAAGAAGGAGAAGA+AGG	+	chr3.4:99685453-99685472	MS.gene063134:intron	35.0%
	TGAAACAGAGATCAAAGACA+GGG	+	chr3.4:99684997-99685016	MS.gene063134:CDS	35.0%
	TGTATTGAATACGTGTCAAG+TGG	+	chr3.4:99685284-99685303	MS.gene063134:intron	35.0%
	TTTGATCTCTGTTTCAGAGT+TGG	-	chr3.4:99684993-99685012	None:intergenic	35.0%
!!	GAGAAAGTTGATGTTGTTGA+TGG	+	chr3.4:99685496-99685515	MS.gene063134:CDS	35.0%
!!	TGCAACTGATTTTAGTCACA+AGG	+	chr3.4:99685087-99685106	MS.gene063134:CDS	35.0%
!!!	AGGAGAGAAGAAAGGTATTT+TGG	+	chr3.4:99685681-99685700	MS.gene063134:CDS	35.0%
!!!	AGGGGTGTTTTTGATTTTCT+AGG	+	chr3.4:99685016-99685035	MS.gene063134:CDS	35.0%
!!!	GGTGTTTTTGATTTTCTAGG+TGG	+	chr3.4:99685019-99685038	MS.gene063134:CDS	35.0%
	ACAACAGCTAGTCATGATCA+AGG	+	chr3.4:99685661-99685680	MS.gene063134:CDS	40.0%
	AGAAAAGAAGGAGAAGAAGG+AGG	+	chr3.4:99685456-99685475	MS.gene063134:intron	40.0%
	AGCTTGAAGAGCTATCAGAT+CGG	-	chr3.4:99685201-99685220	None:intergenic	40.0%
	CATGATCAAGGAGAGAAGAA+AGG	+	chr3.4:99685673-99685692	MS.gene063134:CDS	40.0%
	CATGTTCAGTAGCAGTCTTA+GGG	-	chr3.4:99685738-99685757	None:intergenic	40.0%
	CTGAAACAGAGATCAAAGAC+AGG	+	chr3.4:99684996-99685015	MS.gene063134:CDS	40.0%
	GAAACAGAGATCAAAGACAG+GGG	+	chr3.4:99684998-99685017	MS.gene063134:CDS	40.0%
	TATGCATGTATAGTCGAGTG+AGG	+	chr3.4:99685396-99685415	MS.gene063134:intron	40.0%
	TCATGTTCAGTAGCAGTCTT+AGG	-	chr3.4:99685739-99685758	None:intergenic	40.0%
	TCTTCTACTTTGGTCTCTGA+TGG	-	chr3.4:99685130-99685149	None:intergenic	40.0%
	TGAAGAGAAGAAAGGTTTCC+TGG	+	chr3.4:99685534-99685553	MS.gene063134:CDS	40.0%
!	GCTTCTCCTTAATTTTGTCC+AGG	-	chr3.4:99685555-99685574	None:intergenic	40.0%
	AAAGAAACACACCAGCCTCT+TGG	+	chr3.4:99685165-99685184	MS.gene063134:CDS	45.0%
	ATACGTGTCAAGTGGTAGAG+TGG	+	chr3.4:99685292-99685311	MS.gene063134:intron	45.0%
	ATCGGTGAAGTTTCTCCAAG+AGG	-	chr3.4:99685183-99685202	None:intergenic	45.0%
	GACTGAACGTAGTGTCAACA+AGG	-	chr3.4:99685318-99685337	None:intergenic	45.0%
	GGAGAATCAGAACAAGTACG+AGG	+	chr3.4:99684955-99684974	MS.gene063134:CDS	45.0%
!	CTCTGTTTCAGAGTTGGTTG+CGG	-	chr3.4:99684987-99685006	None:intergenic	45.0%
!	GTTGTTGTTGTCTCAGCAGA+TGG	-	chr3.4:99685643-99685662	None:intergenic	45.0%
!	TCGTCAGTTTTCTTGTGTCC+TGG	-	chr3.4:99685580-99685599	None:intergenic	45.0%
!!	GCAAGCACTGAAGAGAAGAA+AGG	+	chr3.4:99685526-99685545	MS.gene063134:CDS	45.0%
	ACAGCAGGAGCAACAACAAC+AGG	-	chr3.4:99685619-99685638	None:intergenic	50.0%
	GCAGTCTTAGGGTGATAACC+AGG	-	chr3.4:99685727-99685746	None:intergenic	50.0%
	GCATGTATAGTCGAGTGAGG+AGG	+	chr3.4:99685399-99685418	MS.gene063134:intron	50.0%
	GTGAAGTTTCTCCAAGAGGC+TGG	-	chr3.4:99685179-99685198	None:intergenic	50.0%
	GTTGCAACAGCATCCTCTTG+AGG	-	chr3.4:99685073-99685092	None:intergenic	50.0%
	TCGAGTGAGGAGGAAGTTGA+TGG	+	chr3.4:99685409-99685428	MS.gene063134:intron	50.0%
!	TGTTTCAGAGTTGGTTGCGG+TGG	-	chr3.4:99684984-99685003	None:intergenic	50.0%
!!	AGGAAGTTGATGGAGAGAGG+AGG	+	chr3.4:99685419-99685438	MS.gene063134:intron	50.0%
!!	AGGAGGAAGTTGATGGAGAG+AGG	+	chr3.4:99685416-99685435	MS.gene063134:intron	50.0%
	GCAGGAGCAACAACAACAGG+TGG	-	chr3.4:99685616-99685635	None:intergenic	55.0%
	GGAGCAACAACAACAGGTGG+TGG	-	chr3.4:99685613-99685632	None:intergenic	55.0%
	GTCTCAGCAGATGGCACAGC+AGG	-	chr3.4:99685634-99685653	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	99684947	99685801	99684947	ID=MS.gene063134
chr3.4	mRNA	99684947	99685801	99684947	ID=MS.gene063134.t1;Parent=MS.gene063134
chr3.4	exon	99684947	99685226	99684947	ID=MS.gene063134.t1.exon1;Parent=MS.gene063134.t1
chr3.4	CDS	99684947	99685226	99684947	ID=cds.MS.gene063134.t1;Parent=MS.gene063134.t1
chr3.4	exon	99685488	99685801	99685488	ID=MS.gene063134.t1.exon2;Parent=MS.gene063134.t1
chr3.4	CDS	99685488	99685801	99685488	ID=cds.MS.gene063134.t1;Parent=MS.gene063134.t1

Gene Sequence

>MS.gene063134

ATGGCTGAGGAGAATCAGAACAAGTACGAGGAAACCACCGCAACCAACTCTGAAACAGAGATCAAAGACAGGGGTGTTTTTGATTTTCTAGGTGGTAAGAAAAAGGATGAAGAACATATTAAGCCTCAAGAGGATGCTGTTGCAACTGATTTTAGTCACAAGGTGACTTTGTATGAAGCTCCATCAGAGACCAAAGTAGAAGAAAAAGAAGAAGGTGAAAAGAAACACACCAGCCTCTTGGAGAAACTTCACCGATCTGATAGCTCTTCAAGCTCTGTAAGTACTCTTTTTTTTTTTTCATCATTTTCTTAATTTTGTTAGATCATAGTATAGTTAGTGTATTGAATACGTGTCAAGTGGTAGAGTGGCCTTGTTGACACTACGTTCAGTCTTTGTCGCTTTGATTCGTTATATTATGCAAAAATTAATGAATGAATGTTGTTTTGATGTATGCATGTATAGTCGAGTGAGGAGGAAGTTGATGGAGAGAGGAGGAAAAAGAAGAAGAAAGAAAAGAAGGAGAAGAAGGAGGACACATCAGTGCCAGTAGAGAAAGTTGATGTTGTTGATGGAACAACAGCAAGCACTGAAGAGAAGAAAGGTTTCCTGGACAAAATTAAGGAGAAGCTTCCAGGACACAAGAAAACTGACGATGTAACAACTCCACCACCTGTTGTTGTTGCTCCTGCTGTGCCATCTGCTGAGACAACAACAACAACAGCTAGTCATGATCAAGGAGAGAAGAAAGGTATTTTGGAAAAGATCAAAGAGAAGATACCTGGTTATCACCCTAAGACTGCTACTGAACATGAAGACAACAAAGATCATCACAAGGATGAGACTACTTCTCATTGA

Protein sequence

>MS.gene063134.t1

MAEENQNKYEETTATNSETEIKDRGVFDFLGGKKKDEEHIKPQEDAVATDFSHKVTLYEAPSETKVEEKEEGEKKHTSLLEKLHRSDSSSSSVMPVEKVDVVDGTTASTEEKKGFLDKIKEKLPGHKKTDDVTTPPPVVVAPAVPSAETTTTTASHDQGEKKGILEKIKEKIPGYHPKTATEHEDNKDHHKDETTSH