AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68191


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68191.t1 MTR_3g117290 72.398 221 25 7 1 197 1 209 2.74e-71 215
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68191.t1 AT1G76180 44.000 200 76 6 1 184 1 180 1.39e-23 92.8
MS.gene68191.t1 AT1G76180 44.000 200 76 6 1 184 1 180 1.39e-23 92.8
MS.gene68191.t1 AT1G20440 42.233 206 81 11 1 176 1 198 1.42e-19 84.0
MS.gene68191.t1 AT1G20450 36.321 212 87 10 1 175 1 201 3.06e-15 72.4
MS.gene68191.t1 AT1G20450 54.667 75 28 2 110 184 185 253 1.26e-12 65.1
MS.gene68191.t1 AT1G20450 36.019 211 88 10 1 175 1 200 1.92e-14 70.1
MS.gene68191.t1 AT1G20450 55.263 76 26 3 110 184 184 252 1.29e-12 65.1

Find 0 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGAAGAAGAAAGAAAAGA+AGG - 57668:74272-74291 MS.gene68191:intron 25.0%
! AAAGTAGAAGAAAAAGAAGA+AGG - 57668:73958-73977 MS.gene68191:CDS 25.0%
!! TTCTTCTTTTTCTTCTACTT+TGG + 57668:73959-73978 None:intergenic 25.0%
! TTTTCTAGGTGGTAAGAAAA+AGG - 57668:73849-73868 MS.gene68191:CDS 30.0%
AAAATTAAGGAGAAGCTTCC+AGG - 57668:74387-74406 MS.gene68191:CDS 35.0%
AAGATCAAAGAGAAGATACC+TGG - 57668:74534-74553 MS.gene68191:CDS 35.0%
ACAACATCAACTTTCTCTAC+TGG + 57668:74321-74340 None:intergenic 35.0%
AGAACATATTAAGCCTCAAG+AGG - 57668:73876-73895 MS.gene68191:CDS 35.0%
AGACAACAAAGATCATCACA+AGG - 57668:74587-74606 MS.gene68191:CDS 35.0%
AGGTTTCCTGGACAAAATTA+AGG - 57668:74374-74393 MS.gene68191:CDS 35.0%
GAAAGAAAAGAAGGAGAAGA+AGG - 57668:74281-74300 MS.gene68191:intron 35.0%
TGAAACAGAGATCAAAGACA+GGG - 57668:73816-73835 MS.gene68191:CDS 35.0%
TGTATTGAATACGTGTCAAG+TGG - 57668:74104-74123 MS.gene68191:intron 35.0%
TTTGATCTCTGTTTCAGAGT+TGG + 57668:73812-73831 None:intergenic 35.0%
!! GAGAAAGTTGATGTTGTTGA+TGG - 57668:74324-74343 MS.gene68191:intron 35.0%
!! TGCAACTGATTTTAGTCACA+AGG - 57668:73906-73925 MS.gene68191:CDS 35.0%
!!! AGGAGAGAAGAAAGGTATTT+TGG - 57668:74509-74528 MS.gene68191:CDS 35.0%
!!! AGGGGTGTTTTTGATTTTCT+AGG - 57668:73835-73854 MS.gene68191:CDS 35.0%
!!! GGTGTTTTTGATTTTCTAGG+TGG - 57668:73838-73857 MS.gene68191:CDS 35.0%
AGAAAAGAAGGAGAAGAAGG+AGG - 57668:74284-74303 MS.gene68191:intron 40.0%
AGCTTGAAGAGCTATCAGAT+CGG + 57668:74020-74039 None:intergenic 40.0%
CATGTTCAGTAGCAGTCTTA+GGG + 57668:74566-74585 None:intergenic 40.0%
CTGAAACAGAGATCAAAGAC+AGG - 57668:73815-73834 MS.gene68191:CDS 40.0%
GAAACAGAGATCAAAGACAG+GGG - 57668:73817-73836 MS.gene68191:CDS 40.0%
GATTGAACGTAGTGTCAACA+AGG + 57668:74138-74157 None:intergenic 40.0%
TATGCATGTATAGTCGAGTG+AGG - 57668:74224-74243 MS.gene68191:intron 40.0%
TCATGTTCAGTAGCAGTCTT+AGG + 57668:74567-74586 None:intergenic 40.0%
TCTTCTACTTTGGTCTCTGA+TGG + 57668:73949-73968 None:intergenic 40.0%
TGAAGAGAAGAAAGGTTTCC+TGG - 57668:74362-74381 MS.gene68191:CDS 40.0%
! GCTTCTCCTTAATTTTGTCC+AGG + 57668:74383-74402 None:intergenic 40.0%
AAAGAAACACACCAGCCTCT+TGG - 57668:73984-74003 MS.gene68191:CDS 45.0%
ACAACAGCTAGTCACGATCA+AGG - 57668:74489-74508 MS.gene68191:CDS 45.0%
ATCGGTGAAGTTTCTCCAAG+AGG + 57668:74002-74021 None:intergenic 45.0%
CACGATCAAGGAGAGAAGAA+AGG - 57668:74501-74520 MS.gene68191:CDS 45.0%
GGAGAATCAGAACAAGTACG+AGG - 57668:73774-73793 MS.gene68191:CDS 45.0%
! CTCTGTTTCAGAGTTGGTTG+CGG + 57668:73806-73825 None:intergenic 45.0%
! GTTGTTGTTGTCTCAGCAGA+TGG + 57668:74471-74490 None:intergenic 45.0%
! TCGTCAGTTTTCTTGTGTCC+TGG + 57668:74408-74427 None:intergenic 45.0%
!! GCAAGCACTGAAGAGAAGAA+AGG - 57668:74354-74373 MS.gene68191:CDS 45.0%
ACAGCAGGAGCAACAACAAC+AGG + 57668:74447-74466 None:intergenic 50.0%
ATACGTGTCAAGTGGCAGAG+TGG - 57668:74112-74131 MS.gene68191:intron 50.0%
GCAGTCTTAGGGTGATAACC+AGG + 57668:74555-74574 None:intergenic 50.0%
GCATGTATAGTCGAGTGAGG+AGG - 57668:74227-74246 MS.gene68191:intron 50.0%
GTGAAGTTTCTCCAAGAGGC+TGG + 57668:73998-74017 None:intergenic 50.0%
GTTGCAACAGCATCCTCTTG+AGG + 57668:73892-73911 None:intergenic 50.0%
TCGAGTGAGGAGGAAGTTGA+TGG - 57668:74237-74256 MS.gene68191:intron 50.0%
! TGTTTCAGAGTTGGTTGCGG+TGG + 57668:73803-73822 None:intergenic 50.0%
!! AGGAAGTTGATGGAGAGAGG+AGG - 57668:74247-74266 MS.gene68191:intron 50.0%
!! AGGAGGAAGTTGATGGAGAG+AGG - 57668:74244-74263 MS.gene68191:intron 50.0%
GCAGGAGCAACAACAACAGG+TGG + 57668:74444-74463 None:intergenic 55.0%
GGAGCAACAACAACAGGTGG+TGG + 57668:74441-74460 None:intergenic 55.0%
GTCTCAGCAGATGGCACAGC+AGG + 57668:74462-74481 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
57668 gene 73766 74629 73766 ID=MS.gene68191
57668 mRNA 73766 74629 73766 ID=MS.gene68191.t1;Parent=MS.gene68191
57668 exon 74350 74629 74350 ID=MS.gene68191.t1.exon1;Parent=MS.gene68191.t1
57668 CDS 74350 74629 74350 ID=cds.MS.gene68191.t1;Parent=MS.gene68191.t1
57668 exon 73766 74079 73766 ID=MS.gene68191.t1.exon2;Parent=MS.gene68191.t1
57668 CDS 73766 74079 73766 ID=cds.MS.gene68191.t1;Parent=MS.gene68191.t1
Gene Sequence

>MS.gene68191

ATGGCTGAGGAGAATCAGAACAAGTACGAGGAAACCACCGCAACCAACTCTGAAACAGAGATCAAAGACAGGGGTGTTTTTGATTTTCTAGGTGGTAAGAAAAAGGATGAAGAACATATTAAGCCTCAAGAGGATGCTGTTGCAACTGATTTTAGTCACAAGGTGACTTTGTATGAAGCTCCATCAGAGACCAAAGTAGAAGAAAAAGAAGAAGGTGAAAAGAAACACACCAGCCTCTTGGAGAAACTTCACCGATCTGATAGCTCTTCAAGCTCTGTAAGTACTCTTTTTTTTTTCTTCATCATTTTCTTAATTTTGTTAGATCATAGTATAGTTAGTGTATTGAATACGTGTCAAGTGGCAGAGTGGCCTTGTTGACACTACGTTCAATCATTCAGTCTTTGTCGCTTTGATTCGTTATATTATGCAAAAATTAATGAATGAATGTTGTTTTGATGTATGCATGTATAGTCGAGTGAGGAGGAAGTTGATGGAGAGAGGAGGAAAAAGAAGAAGAAAGAAAAGAAGGAGAAGAAGGAGGACACATCAGTGCCAGTAGAGAAAGTTGATGTTGTTGATGGAACAACAGCAAGCACTGAAGAGAAGAAAGGTTTCCTGGACAAAATTAAGGAGAAGCTTCCAGGACACAAGAAAACTGACGATGTAACAACTCCACCACCTGTTGTTGTTGCTCCTGCTGTGCCATCTGCTGAGACAACAACAACAACAGCTAGTCACGATCAAGGAGAGAAGAAAGGTATTTTGGAAAAGATCAAAGAGAAGATACCTGGTTATCACCCTAAGACTGCTACTGAACATGAAGACAACAAAGATCATCACAAGGATGAGACTACTTCTCATTGA

Protein sequence

>MS.gene68191.t1

MAEENQNKYEETTATNSETEIKDRGVFDFLGGKKKDEEHIKPQEDAVATDFSHKVTLYEAPSETKVEEKEEGEKKHTSLLEKLHRSDSSSSSVMPVEKVDVVDGTTASTEEKKGFLDKIKEKLPGHKKTDDVTTPPPVVVAPAVPSAETTTTTASHDQGEKKGILEKIKEKIPGYHPKTATEHEDNKDHHKDETTSH