Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014250.t1 | KEH36372.1 | 99.5 | 210 | 1 | 0 | 1 | 210 | 1 | 210 | 4.10E-121 | 444.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014250.t1 | Q9FEN9 | 70.5 | 210 | 59 | 2 | 1 | 210 | 1 | 207 | 3.3e-87 | 323.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014250.t1 | G7J7R0 | 99.5 | 210 | 1 | 0 | 1 | 210 | 1 | 210 | 2.9e-121 | 444.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene014250.t1 | TR | PHD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene059748 | MS.gene014250 | PPI |
| MS.gene014250 | MS.gene58386 | PPI |
| MS.gene014250 | MS.gene062590 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014250.t1 | MTR_3g117270 | 99.524 | 210 | 1 | 0 | 1 | 210 | 1 | 210 | 1.24e-157 | 442 |
| MS.gene014250.t1 | MTR_3g117270 | 68.205 | 195 | 58 | 1 | 211 | 401 | 17 | 211 | 1.62e-95 | 285 |
| MS.gene014250.t1 | MTR_3g117270 | 99.524 | 210 | 1 | 0 | 1 | 210 | 1 | 210 | 1.24e-157 | 443 |
| MS.gene014250.t1 | MTR_3g117270 | 69.307 | 202 | 58 | 1 | 211 | 408 | 17 | 218 | 1.09e-100 | 298 |
| MS.gene014250.t1 | MTR_3g117280 | 90.816 | 196 | 18 | 0 | 213 | 408 | 1 | 196 | 9.62e-133 | 379 |
| MS.gene014250.t1 | MTR_3g117280 | 70.681 | 191 | 52 | 1 | 19 | 209 | 1 | 187 | 6.15e-96 | 285 |
| MS.gene014250.t1 | MTR_3g117270 | 99.394 | 165 | 1 | 0 | 1 | 165 | 1 | 165 | 4.11e-121 | 348 |
| MS.gene014250.t1 | MTR_3g117270 | 65.772 | 149 | 47 | 1 | 211 | 355 | 17 | 165 | 2.62e-67 | 211 |
| MS.gene014250.t1 | MTR_3g117270 | 99.390 | 164 | 1 | 0 | 1 | 164 | 1 | 164 | 7.48e-120 | 345 |
| MS.gene014250.t1 | MTR_3g117270 | 65.541 | 148 | 47 | 1 | 211 | 354 | 17 | 164 | 4.57e-66 | 208 |
| MS.gene014250.t1 | MTR_2g078310 | 73.158 | 190 | 48 | 2 | 1 | 190 | 1 | 187 | 1.26e-97 | 291 |
| MS.gene014250.t1 | MTR_2g078310 | 54.922 | 193 | 83 | 2 | 220 | 408 | 24 | 216 | 3.83e-72 | 225 |
| MS.gene014250.t1 | MTR_2g090365 | 50.521 | 192 | 87 | 5 | 1 | 190 | 263 | 448 | 3.20e-57 | 194 |
| MS.gene014250.t1 | MTR_2g090365 | 49.714 | 175 | 82 | 3 | 215 | 384 | 279 | 452 | 6.72e-52 | 180 |
| MS.gene014250.t1 | MTR_5g097190 | 45.968 | 124 | 58 | 4 | 74 | 188 | 15 | 138 | 9.81e-27 | 110 |
| MS.gene014250.t1 | MTR_5g097190 | 43.750 | 128 | 63 | 3 | 260 | 378 | 11 | 138 | 7.93e-25 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014250.t1 | AT4G39100 | 70.476 | 210 | 59 | 2 | 1 | 210 | 1 | 207 | 8.88e-110 | 322 |
| MS.gene014250.t1 | AT4G39100 | 58.639 | 191 | 74 | 2 | 219 | 405 | 23 | 212 | 6.73e-80 | 246 |
| MS.gene014250.t1 | AT4G22140 | 67.143 | 210 | 65 | 3 | 2 | 210 | 10 | 216 | 9.20e-103 | 304 |
| MS.gene014250.t1 | AT4G22140 | 56.477 | 193 | 80 | 2 | 220 | 408 | 32 | 224 | 5.32e-78 | 241 |
| MS.gene014250.t1 | AT4G22140 | 67.143 | 210 | 65 | 3 | 2 | 210 | 10 | 216 | 1.76e-102 | 303 |
| MS.gene014250.t1 | AT4G22140 | 55.914 | 186 | 78 | 2 | 220 | 401 | 32 | 217 | 1.23e-73 | 230 |
| MS.gene014250.t1 | AT4G22140 | 67.143 | 210 | 65 | 3 | 2 | 210 | 10 | 216 | 1.76e-102 | 303 |
| MS.gene014250.t1 | AT4G22140 | 55.914 | 186 | 78 | 2 | 220 | 401 | 32 | 217 | 1.23e-73 | 230 |
| MS.gene014250.t1 | AT4G39100 | 76.364 | 165 | 37 | 1 | 1 | 165 | 1 | 163 | 7.91e-93 | 276 |
| MS.gene014250.t1 | AT4G39100 | 62.411 | 141 | 49 | 1 | 219 | 355 | 23 | 163 | 3.87e-62 | 198 |
| MS.gene014250.t1 | AT4G04260 | 62.162 | 185 | 66 | 3 | 28 | 210 | 9 | 191 | 1.21e-75 | 234 |
| MS.gene014250.t1 | AT4G04260 | 48.913 | 184 | 88 | 3 | 222 | 400 | 9 | 191 | 3.22e-56 | 184 |
| MS.gene014250.t1 | AT4G04260 | 61.111 | 180 | 66 | 3 | 33 | 210 | 1 | 178 | 1.77e-71 | 223 |
| MS.gene014250.t1 | AT4G04260 | 48.045 | 179 | 87 | 3 | 227 | 400 | 1 | 178 | 4.05e-53 | 176 |
| MS.gene014250.t1 | AT4G04260 | 71.186 | 118 | 33 | 1 | 25 | 142 | 24 | 140 | 5.87e-55 | 178 |
| MS.gene014250.t1 | AT4G04260 | 56.034 | 116 | 48 | 1 | 220 | 332 | 25 | 140 | 7.37e-39 | 136 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAGGAATACCTTTGTATT+TGG | 0.149988 | 3.3:-95234142 | None:intergenic |
| ATGACTCTAGGTTTCGCTTT+GGG | 0.256713 | 3.3:-95225465 | None:intergenic |
| AGGTTTCGCTTTGGGGCTTT+TGG | 0.301619 | 3.3:-95225457 | None:intergenic |
| AATGAAGTTGTCAATTCTTT+CGG | 0.316759 | 3.3:-95234056 | None:intergenic |
| CTTTGCCACGTAAGAGGGTT+TGG | 0.340256 | 3.3:-95225643 | None:intergenic |
| GAAGGAAAGTGTATTGTTCA+TGG | 0.347757 | 3.3:+95225825 | MS.gene014250:CDS |
| TTAGGAATACCTTTGTATTT+GGG | 0.355673 | 3.3:-95234141 | None:intergenic |
| AAAGAAGTGGTCAAGTCTTT+CGG | 0.363321 | 3.3:-95228281 | None:intergenic |
| ACTCACACTGCAACTCTATC+AGG | 0.365443 | 3.3:-95225936 | None:intergenic |
| ACCATCACTTTCATCTTTGA+CGG | 0.371838 | 3.3:-95234989 | None:intergenic |
| TTAACGTCCGGTGGTATTAC+CGG | 0.372519 | 3.3:+95225709 | MS.gene014250:CDS |
| AAAATGCACTGTTCACAGTT+TGG | 0.374580 | 3.3:+95232420 | MS.gene014250:CDS |
| CACTGAACCATCAGATCATC+AGG | 0.376018 | 3.3:-95226721 | None:intergenic |
| TGTGAAAGTTGTTCCGTTGA+AGG | 0.381340 | 3.3:+95228304 | MS.gene014250:CDS |
| CACTGGATCATAACTTCATC+AGG | 0.392678 | 3.3:-95232730 | None:intergenic |
| CATAACTTCATCAGGGTTGT+AGG | 0.393557 | 3.3:-95232722 | None:intergenic |
| TTCGTGTCCGTTGGTACTAC+TGG | 0.395721 | 3.3:+95232299 | MS.gene014250:CDS |
| ACTCACACTTGAACTATATC+AGG | 0.399463 | 3.3:-95232526 | None:intergenic |
| TGTTGGTATCCACGGTTTAG+AGG | 0.405406 | 3.3:-95232233 | None:intergenic |
| CATCAGATCATCAGGGTTGT+AGG | 0.418034 | 3.3:-95226713 | None:intergenic |
| TTCGATCTTTGCCACGTAAG+AGG | 0.444854 | 3.3:-95225649 | None:intergenic |
| AACGGCATATCTGAATTCAT+TGG | 0.448031 | 3.3:-95232055 | None:intergenic |
| TATGACTCTAGGTTTCGCTT+TGG | 0.453882 | 3.3:-95225466 | None:intergenic |
| TTGAACTACAATTCCGCTAC+TGG | 0.456893 | 3.3:+95232493 | MS.gene014250:CDS |
| TTTGAGTACAATTCTGCTAC+TGG | 0.478917 | 3.3:+95225903 | MS.gene014250:CDS |
| CAGAGTGCTGATACAATTGA+AGG | 0.496733 | 3.3:+95225807 | MS.gene014250:CDS |
| AGTGTGAGGGCTGCAGTGAC+TGG | 0.500549 | 3.3:+95226740 | MS.gene014250:CDS |
| TCGATCTTTGCCACGTAAGA+GGG | 0.507398 | 3.3:-95225648 | None:intergenic |
| TGACTCTAGGTTTCGCTTTG+GGG | 0.508250 | 3.3:-95225464 | None:intergenic |
| GCCACGTAAGAGGGTTTGGA+TGG | 0.509773 | 3.3:-95225639 | None:intergenic |
| GAGACAGTTTCACGGTTCAA+AGG | 0.514600 | 3.3:+95225755 | MS.gene014250:CDS |
| TACCGGCCGGAGGAGTCAAT+TGG | 0.521187 | 3.3:+95225726 | MS.gene014250:CDS |
| AACAAATCTGTCACAATCAA+AGG | 0.523901 | 3.3:+95225505 | MS.gene014250:CDS |
| ATCAGATCATCAGGGTTGTA+GGG | 0.525800 | 3.3:-95226712 | None:intergenic |
| CGACCCTTCTTTGTCTCCTC+CGG | 0.527955 | 3.3:-95232322 | None:intergenic |
| TACATGAAGCTTGATGTTGT+TGG | 0.528797 | 3.3:+95232448 | MS.gene014250:CDS |
| AGTGTGATCGCTGCACTGAT+TGG | 0.536011 | 3.3:+95232749 | MS.gene014250:CDS |
| GCCACATATGTTGGTATCCA+CGG | 0.537762 | 3.3:-95232241 | None:intergenic |
| CGGCCACCAATTGACTCCTC+CGG | 0.542045 | 3.3:-95225732 | None:intergenic |
| CTACAACCCTGATGATCTGA+TGG | 0.544719 | 3.3:+95226714 | MS.gene014250:CDS |
| TGATCTGATGGTTCAGTGTG+AGG | 0.546259 | 3.3:+95226726 | MS.gene014250:CDS |
| CAAGAGATGTTCGTGTCCGT+TGG | 0.548163 | 3.3:+95232290 | MS.gene014250:CDS |
| ATAACTTCATCAGGGTTGTA+GGG | 0.552648 | 3.3:-95232721 | None:intergenic |
| CGGCCGGAGGAGTCAATTGG+TGG | 0.557866 | 3.3:+95225729 | MS.gene014250:CDS |
| GAGGGTTTGGATGGATCAGA+TGG | 0.563022 | 3.3:-95225630 | None:intergenic |
| TACACTAAGCTTGATGCTGT+TGG | 0.564424 | 3.3:+95225858 | MS.gene014250:CDS |
| CGGAGGAGTCAATTGGTGGC+CGG | 0.567621 | 3.3:+95225733 | MS.gene014250:CDS |
| GTTTGACTGAGTATGACTCT+AGG | 0.568800 | 3.3:-95225477 | None:intergenic |
| ACCGTCATGTCTATACAAGC+AGG | 0.569799 | 3.3:-95234025 | None:intergenic |
| AAGGGTCGGCGTCATTTCCA+TGG | 0.570085 | 3.3:+95232337 | MS.gene014250:CDS |
| GGTGGCCGGAGACAGTTTCA+CGG | 0.570440 | 3.3:+95225747 | MS.gene014250:CDS |
| CTGGCCGGAGGAGACAAAGA+AGG | 0.572097 | 3.3:+95232318 | MS.gene014250:CDS |
| ACCGTGGATACCAACATATG+TGG | 0.579459 | 3.3:+95232240 | MS.gene014250:CDS |
| TGTCCGTTGGTACTACTGGC+CGG | 0.580901 | 3.3:+95232303 | MS.gene014250:CDS |
| ACAGTCATGTCTATACAAGC+AGG | 0.584679 | 3.3:-95228250 | None:intergenic |
| CACCAATTGACTCCTCCGGC+CGG | 0.585767 | 3.3:-95225728 | None:intergenic |
| TCTTACGTGGCAAAGATCGA+AGG | 0.586509 | 3.3:+95225651 | MS.gene014250:CDS |
| TCGATCATAGCCACATATGT+TGG | 0.588140 | 3.3:-95232250 | None:intergenic |
| AACAACTTTCACAAAAGAAG+TGG | 0.593717 | 3.3:-95228294 | None:intergenic |
| GCGTCATTTCCATGGCTCAA+AGG | 0.593809 | 3.3:+95232345 | MS.gene014250:CDS |
| TTGAACCGTGAAACTGTCTC+CGG | 0.596615 | 3.3:-95225752 | None:intergenic |
| GATTAGAACTTACTACGGAA+CGG | 0.601353 | 3.3:-95232073 | None:intergenic |
| GATCTGATGGTTCAGTGTGA+GGG | 0.605136 | 3.3:+95226727 | MS.gene014250:CDS |
| CGGAGGAGACAAAGAAGGGT+CGG | 0.615656 | 3.3:+95232323 | MS.gene014250:CDS |
| CCTCCGGCCGGTAATACCAC+CGG | 0.617818 | 3.3:-95225716 | None:intergenic |
| TCCTGCTTGTATAGACATGA+CGG | 0.620123 | 3.3:+95234024 | MS.gene014250:CDS |
| CTAACGTTAAAGTTAACGTC+CGG | 0.628228 | 3.3:+95225697 | MS.gene014250:CDS |
| AATCAGTGCAGCGATCACAC+TGG | 0.630754 | 3.3:-95232747 | None:intergenic |
| TACAAGACTCGCAGATACAA+AGG | 0.635448 | 3.3:+95228357 | MS.gene014250:CDS |
| TGGCCGGAGGAGACAAAGAA+GGG | 0.641254 | 3.3:+95232319 | MS.gene014250:CDS |
| CAGAGTGTTGATACAATCGA+AGG | 0.642499 | 3.3:+95232397 | MS.gene014250:CDS |
| AGGAGTGAGAGCACCAGTAG+CGG | 0.642633 | 3.3:-95232506 | None:intergenic |
| CGTCCGGTGGTATTACCGGC+CGG | 0.645283 | 3.3:+95225713 | MS.gene014250:CDS |
| CTTACGTGGCAAAGATCGAA+GGG | 0.647189 | 3.3:+95225652 | MS.gene014250:CDS |
| CCTCCGGCCAGTAGTACCAA+CGG | 0.658998 | 3.3:-95232306 | None:intergenic |
| TGCAAGACTCCCAAATACAA+AGG | 0.662219 | 3.3:+95234132 | MS.gene014250:CDS |
| TCCATCCAAACCCTCTTACG+TGG | 0.664323 | 3.3:+95225638 | MS.gene014250:CDS |
| CCGGTGGTATTACCGGCCGG+AGG | 0.671639 | 3.3:+95225716 | MS.gene014250:CDS |
| ACTGGATCATAACTTCATCA+GGG | 0.673831 | 3.3:-95232729 | None:intergenic |
| ACGTTAAAGTTAACGTCCGG+TGG | 0.681208 | 3.3:+95225700 | MS.gene014250:CDS |
| TTGTATAGACATGACTGTGG+AGG | 0.686994 | 3.3:+95228255 | MS.gene014250:CDS |
| GGGATCGAAGCTGATTCACG+AGG | 0.707404 | 3.3:+95225672 | MS.gene014250:CDS |
| ACTGAACCATCAGATCATCA+GGG | 0.710984 | 3.3:-95226720 | None:intergenic |
| GCATGCGTTCCTCTAAACCG+TGG | 0.713047 | 3.3:+95232224 | MS.gene014250:CDS |
| CCGTTGGTACTACTGGCCGG+AGG | 0.722355 | 3.3:+95232306 | MS.gene014250:CDS |
| TGCTTGTATAGACATGACGG+TGG | 0.750089 | 3.3:+95234027 | MS.gene014250:CDS |
| TGCTTGTATAGACATGACTG+TGG | 0.776325 | 3.3:+95228252 | MS.gene014250:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 95225448 | 95235001 | 95225448 | ID=MS.gene014250 |
| chr3.3 | mRNA | 95225448 | 95235001 | 95225448 | ID=MS.gene014250.t1;Parent=MS.gene014250 |
| chr3.3 | exon | 95225448 | 95225526 | 95225448 | ID=MS.gene014250.t1.exon1;Parent=MS.gene014250.t1 |
| chr3.3 | CDS | 95225448 | 95225526 | 95225448 | ID=cds.MS.gene014250.t1;Parent=MS.gene014250.t1 |
| chr3.3 | exon | 95225607 | 95225952 | 95225607 | ID=MS.gene014250.t1.exon2;Parent=MS.gene014250.t1 |
| chr3.3 | CDS | 95225607 | 95225952 | 95225607 | ID=cds.MS.gene014250.t1;Parent=MS.gene014250.t1 |
| chr3.3 | exon | 95226693 | 95226761 | 95226693 | ID=MS.gene014250.t1.exon3;Parent=MS.gene014250.t1 |
| chr3.3 | CDS | 95226693 | 95226761 | 95226693 | ID=cds.MS.gene014250.t1;Parent=MS.gene014250.t1 |
| chr3.3 | exon | 95228243 | 95228378 | 95228243 | ID=MS.gene014250.t1.exon4;Parent=MS.gene014250.t1 |
| chr3.3 | CDS | 95228243 | 95228378 | 95228243 | ID=cds.MS.gene014250.t1;Parent=MS.gene014250.t1 |
| chr3.3 | exon | 95232052 | 95232082 | 95232052 | ID=MS.gene014250.t1.exon5;Parent=MS.gene014250.t1 |
| chr3.3 | CDS | 95232052 | 95232082 | 95232052 | ID=cds.MS.gene014250.t1;Parent=MS.gene014250.t1 |
| chr3.3 | exon | 95232209 | 95232542 | 95232209 | ID=MS.gene014250.t1.exon6;Parent=MS.gene014250.t1 |
| chr3.3 | CDS | 95232209 | 95232542 | 95232209 | ID=cds.MS.gene014250.t1;Parent=MS.gene014250.t1 |
| chr3.3 | exon | 95232702 | 95232770 | 95232702 | ID=MS.gene014250.t1.exon7;Parent=MS.gene014250.t1 |
| chr3.3 | CDS | 95232702 | 95232770 | 95232702 | ID=cds.MS.gene014250.t1;Parent=MS.gene014250.t1 |
| chr3.3 | exon | 95234018 | 95234153 | 95234018 | ID=MS.gene014250.t1.exon8;Parent=MS.gene014250.t1 |
| chr3.3 | CDS | 95234018 | 95234153 | 95234018 | ID=cds.MS.gene014250.t1;Parent=MS.gene014250.t1 |
| chr3.3 | exon | 95234975 | 95235001 | 95234975 | ID=MS.gene014250.t1.exon9;Parent=MS.gene014250.t1 |
| chr3.3 | CDS | 95234975 | 95235001 | 95234975 | ID=cds.MS.gene014250.t1;Parent=MS.gene014250.t1 |
| Gene Sequence |
| Protein sequence |
