AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062590.t1 MTR_2g078310 99.468 188 1 0 1 188 31 218 4.45e-142 394
MS.gene062590.t1 MTR_3g117270 70.588 187 53 2 1 186 33 218 1.26e-99 287
MS.gene062590.t1 MTR_3g117270 70.556 180 51 2 1 179 33 211 7.72e-96 277
MS.gene062590.t1 MTR_3g117270 80.451 133 25 1 1 132 33 165 4.52e-80 235
MS.gene062590.t1 MTR_3g117270 80.303 132 25 1 1 131 33 164 1.14e-78 232
MS.gene062590.t1 MTR_3g117280 57.527 186 75 2 1 186 15 196 1.77e-76 228
MS.gene062590.t1 MTR_2g090365 65.217 161 53 2 1 159 291 450 5.71e-67 211
MS.gene062590.t1 MTR_5g097190 47.287 129 58 4 41 159 15 143 6.91e-32 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062590.t1 AT4G22140 88.172 186 22 0 1 186 39 224 1.02e-125 353
MS.gene062590.t1 AT4G22140 88.268 179 21 0 1 179 39 217 2.62e-121 342
MS.gene062590.t1 AT4G22140 88.268 179 21 0 1 179 39 217 2.62e-121 342
MS.gene062590.t1 AT4G04260 74.860 179 43 2 1 178 1 178 1.10e-92 268
MS.gene062590.t1 AT4G04260 74.860 179 43 2 1 178 14 191 1.26e-92 269
MS.gene062590.t1 AT4G39100 66.304 184 59 2 1 183 31 212 1.08e-87 257
MS.gene062590.t1 AT4G39100 74.436 133 33 1 1 132 31 163 1.75e-72 216
MS.gene062590.t1 AT4G04260 85.321 109 16 0 1 109 32 140 5.35e-65 196

Find 49 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCGAAGAAGCAAAGAAAT+TGG 0.293372 2.1:+22195472 MS.gene062590:CDS
ATTAGGTCATCCGGGTTATA+AGG 0.334913 2.1:-22194716 None:intergenic
CTTCCACCAATAGATTCTTC+AGG 0.349796 2.1:-22194353 None:intergenic
CACTGCACCATTAGGTCATC+CGG 0.369370 2.1:-22194725 None:intergenic
CTATCAGATCCTGGTGATAC+AGG 0.385003 2.1:-22195557 None:intergenic
AACATTCAGAACAAACAAAA+TGG 0.403224 2.1:-22195496 None:intergenic
AGAAGATCGAGCAAGATAAT+AGG 0.403757 2.1:+22194294 MS.gene062590:CDS
GTTCTGAGGATTACTATTGT+AGG 0.415271 2.1:+22194501 MS.gene062590:CDS
ACCCTTCACACTGCACCATT+AGG 0.433071 2.1:-22194733 None:intergenic
GGTGGAAGAAGACAGTTTCA+TGG 0.437599 2.1:+22194368 MS.gene062590:CDS
CAAAGTGCTCACACTATTGA+AGG 0.439100 2.1:+22194428 MS.gene062590:CDS
TTATAACCCGGATGACCTAA+TGG 0.443180 2.1:+22194718 MS.gene062590:CDS
GGTCCTCTCTTTCGCCGCTT+CGG 0.470540 2.1:-22197133 None:intergenic
CTCACCTTGCTATCAGATCC+TGG 0.485170 2.1:-22195566 None:intergenic
CTTCTCCACACGTGCCACAT+AGG 0.490109 2.1:-22194276 None:intergenic
GAGCACTTTGAACATCAAAA+TGG 0.494985 2.1:-22194415 None:intergenic
TATAGACCTGAAGAATCTAT+TGG 0.496809 2.1:+22194347 MS.gene062590:CDS
TAGGTACCATCCTGCTTGTG+TGG 0.503860 2.1:+22195442 MS.gene062590:intron
ACTCACACAGCAACACGGTC+AGG 0.504648 2.1:-22194557 None:intergenic
TTTGAGTATAAAGCTGCTAC+TGG 0.508499 2.1:+22194524 MS.gene062590:CDS
AAGACAGTTTCATGGAGCTA+AGG 0.520921 2.1:+22194376 MS.gene062590:CDS
TTCTCCACACGTGCCACATA+GGG 0.531360 2.1:-22194275 None:intergenic
GAGCCGAAGCGGCGAAAGAG+AGG 0.538999 2.1:+22197130 MS.gene062590:CDS
TATACTAAGCTTGAGAATGT+AGG 0.549874 2.1:+22194479 MS.gene062590:CDS
GACCTAATGGTGCAGTGTGA+AGG 0.564361 2.1:+22194731 MS.gene062590:CDS
TTCTGCAGGTGGAGCCGAAG+CGG 0.566551 2.1:+22197119 MS.gene062590:intron
AAGATAATAGGAACAATGTG+AGG 0.571957 2.1:+22194306 MS.gene062590:CDS
GCTTGAGAATGTAGGTTCTG+AGG 0.572249 2.1:+22194487 MS.gene062590:CDS
ATAGTCATGCCCACACAAGC+AGG 0.572617 2.1:-22195452 None:intergenic
CACGAGTAAGCCACCCTATG+TGG 0.577004 2.1:+22194262 MS.gene062590:CDS
AGTGTGAAGGGTGCAAGGAT+TGG 0.587494 2.1:+22194744 MS.gene062590:CDS
GCCACCCTATGTGGCACGTG+TGG 0.588251 2.1:+22194271 MS.gene062590:CDS
ATGCGAGATGCCTTATAACC+CGG 0.597197 2.1:+22194706 MS.gene062590:CDS
TCCACACGTGCCACATAGGG+TGG 0.599622 2.1:-22194272 None:intergenic
AGACCTGAAGAATCTATTGG+TGG 0.602382 2.1:+22194350 MS.gene062590:CDS
ACCTAATGGTGCAGTGTGAA+GGG 0.605001 2.1:+22194732 MS.gene062590:CDS
ACTGCACCATTAGGTCATCC+GGG 0.612849 2.1:-22194724 None:intergenic
CGATGATTTGAAGAAACCAC+AGG 0.614767 2.1:+22195526 MS.gene062590:CDS
AGGTACCATCCTGCTTGTGT+GGG 0.617835 2.1:+22195443 MS.gene062590:intron
GCGGCGTTTCCTGTATCACC+AGG 0.621923 2.1:+22195548 MS.gene062590:CDS
ATCACCAGGATCTGATAGCA+AGG 0.624737 2.1:+22195562 MS.gene062590:CDS
ATGTGAGGGTTCGTGTGAGA+TGG 0.629136 2.1:+22194321 MS.gene062590:CDS
GATACAGGAAACGCCGCCTG+TGG 0.630270 2.1:-22195542 None:intergenic
GGTGGCTTACTCGTGTCAGA+AGG 0.633090 2.1:-22194254 None:intergenic
AAAGTGCTCACACTATTGAA+GGG 0.633847 2.1:+22194429 MS.gene062590:CDS
GGTGCAGTGTGAAGGGTGCA+AGG 0.638726 2.1:+22194739 MS.gene062590:CDS
AGATAATAGGAACAATGTGA+GGG 0.647787 2.1:+22194307 MS.gene062590:CDS
TCATGCCCACACAAGCAGGA+TGG 0.663384 2.1:-22195448 None:intergenic
TGATTTGAAGAAACCACAGG+CGG 0.754665 2.1:+22195529 MS.gene062590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATTTACAAAAATTTA+CGG - chr2.1:22196356-22196375 None:intergenic 10.0%
!! CATATATTCTAATTAAAATT+TGG + chr2.1:22195757-22195776 MS.gene062590:intron 10.0%
!! TAGAAAAAGATAAAAAAAAA+AGG - chr2.1:22196637-22196656 None:intergenic 10.0%
!! TGAAAAAAAATAACATATAT+GGG + chr2.1:22196127-22196146 MS.gene062590:intron 10.0%
!! TTGAAAAAAAATAACATATA+TGG + chr2.1:22196126-22196145 MS.gene062590:intron 10.0%
!! TCAAATTTACAAAAATTTAC+GGG - chr2.1:22196355-22196374 None:intergenic 15.0%
!!! AAAGAATTTTGAGAAAAAAA+CGG - chr2.1:22195828-22195847 None:intergenic 15.0%
!!! ACCACATTTAAAATTTATAA+AGG - chr2.1:22195949-22195968 None:intergenic 15.0%
!!! ACCTTTATAAATTTTAAATG+TGG + chr2.1:22195945-22195964 MS.gene062590:intron 15.0%
!!! ATTTAGTTCTAAATATTATG+AGG + chr2.1:22195610-22195629 MS.gene062590:intron 15.0%
!!! ATTTTGTGAGTAATAAATAA+TGG + chr2.1:22196944-22196963 MS.gene062590:intron 15.0%
!!! TTTATTTTTTTCCAATCTAT+AGG + chr2.1:22195215-22195234 MS.gene062590:intron 15.0%
!! CAAATGCTAAAATTAGATAT+CGG - chr2.1:22194821-22194840 None:intergenic 20.0%
!! TAATCAGTAGTTAATATCTA+GGG + chr2.1:22196433-22196452 MS.gene062590:intron 20.0%
!! TACCTTTCATAAAATATAGA+GGG - chr2.1:22195397-22195416 None:intergenic 20.0%
!! TCATAAATTGAATTATGCAA+GGG + chr2.1:22195238-22195257 MS.gene062590:intron 20.0%
!! TTATATTTAAGCTTATCTGA+TGG + chr2.1:22196472-22196491 MS.gene062590:intron 20.0%
!! TTCAAACAATGAAAAACAAT+TGG - chr2.1:22195127-22195146 None:intergenic 20.0%
!!! AATTTTAGCTCTTGTATTAA+CGG - chr2.1:22195086-22195105 None:intergenic 20.0%
!!! ACATAATCTCTTTTAATCAA+TGG + chr2.1:22197002-22197021 MS.gene062590:intron 20.0%
!!! ATTATTTTGATTGACACTAT+AGG + chr2.1:22197048-22197067 MS.gene062590:intron 20.0%
!!! GCAAAAGATTATTGAATTTT+TGG + chr2.1:22195322-22195341 MS.gene062590:intron 20.0%
!!! GTTGTTTTTATAAGCAATAT+TGG - chr2.1:22196589-22196608 None:intergenic 20.0%
!!! TCAAGTTTTTTATTCCTTTT+TGG + chr2.1:22196979-22196998 MS.gene062590:intron 20.0%
!!! TTATTTTGATTGACACTATA+GGG + chr2.1:22197049-22197068 MS.gene062590:intron 20.0%
!!! TTTTGAATAATTTGCTGATT+TGG + chr2.1:22194658-22194677 MS.gene062590:intron 20.0%
!!! TTTTGATGAATGAATATATG+TGG + chr2.1:22194862-22194881 MS.gene062590:intron 20.0%
! AAAAGAGATTATGTCCAAAA+AGG - chr2.1:22196996-22197015 None:intergenic 25.0%
! AAACAATGAAAAACAATTGG+AGG - chr2.1:22195124-22195143 None:intergenic 25.0%
! AAAGTAAAACAGATAAATGC+TGG + chr2.1:22195029-22195048 MS.gene062590:intron 25.0%
! AACATTCAGAACAAACAAAA+TGG - chr2.1:22195499-22195518 None:intergenic 25.0%
! AATAAAGAACCAAAAACGAA+AGG - chr2.1:22194596-22194615 None:intergenic 25.0%
! AATCAGTAGTTAATATCTAG+GGG + chr2.1:22196434-22196453 MS.gene062590:intron 25.0%
! AATGCTGCAATATGATTAAA+AGG + chr2.1:22195895-22195914 MS.gene062590:intron 25.0%
! ATGCTGCAATATGATTAAAA+GGG + chr2.1:22195896-22195915 MS.gene062590:intron 25.0%
! ATTGACATCAGAAATTATCT+AGG + chr2.1:22196050-22196069 MS.gene062590:intron 25.0%
! CAAATACTCAAAACTCAATT+AGG - chr2.1:22194621-22194640 None:intergenic 25.0%
! GTAATCAGTAGTTAATATCT+AGG + chr2.1:22196432-22196451 MS.gene062590:intron 25.0%
! GTACCTTTCATAAAATATAG+AGG - chr2.1:22195398-22195417 None:intergenic 25.0%
! GTCATAAATTGAATTATGCA+AGG + chr2.1:22195237-22195256 MS.gene062590:intron 25.0%
! TACCAAGGTAAAAGATAAAA+CGG - chr2.1:22196238-22196257 None:intergenic 25.0%
! TCAATTTATGACCTATAGAT+TGG - chr2.1:22195229-22195248 None:intergenic 25.0%
! TGTAATACATTCTCTGTTAT+TGG + chr2.1:22194963-22194982 MS.gene062590:intron 25.0%
! TGTGAAATATAGTTCGAAAA+AGG - chr2.1:22196287-22196306 None:intergenic 25.0%
! TTGTGAGTAATAAATAATGG+AGG + chr2.1:22196947-22196966 MS.gene062590:intron 25.0%
! TTTAATCATATTGCAGCATT+AGG - chr2.1:22195895-22195914 None:intergenic 25.0%
!! ATTCTAACAATTTGAAGCTT+TGG + chr2.1:22196813-22196832 MS.gene062590:intron 25.0%
!! GGATAGAGTTTAAAACAAAA+GGG - chr2.1:22196217-22196236 None:intergenic 25.0%
!! TGGCTTTTACAAGAAAATAA+AGG - chr2.1:22196911-22196930 None:intergenic 25.0%
!! TTCCCTCTATATTTTATGAA+AGG + chr2.1:22195392-22195411 MS.gene062590:intron 25.0%
!! TTGGTGATCTGATAAAATAA+GGG + chr2.1:22196837-22196856 MS.gene062590:intron 25.0%
!! TTTGGTGATCTGATAAAATA+AGG + chr2.1:22196836-22196855 MS.gene062590:intron 25.0%
!!! AAATGTGGTTTTGATATCTA+TGG + chr2.1:22195960-22195979 MS.gene062590:intron 25.0%
!!! ATATGCTATATTGGCTTTTT+AGG + chr2.1:22195360-22195379 MS.gene062590:intron 25.0%
!!! CGATATCTAATTTTAGCATT+TGG + chr2.1:22194819-22194838 MS.gene062590:intron 25.0%
!!! TTTGAGTATTTGATTTGATG+AGG + chr2.1:22194629-22194648 MS.gene062590:intron 25.0%
AAATATAAGCCAATCCAAAC+AGG - chr2.1:22196460-22196479 None:intergenic 30.0%
AACAGATAAATGCTGGAAAT+AGG + chr2.1:22195036-22195055 MS.gene062590:intron 30.0%
AAGATAATAGGAACAATGTG+AGG + chr2.1:22194306-22194325 MS.gene062590:CDS 30.0%
ACAAAATGAACACTGCTTAA+TGG - chr2.1:22196931-22196950 None:intergenic 30.0%
ACAGATAAATGCTGGAAATA+GGG + chr2.1:22195037-22195056 MS.gene062590:intron 30.0%
AGATAATAGGAACAATGTGA+GGG + chr2.1:22194307-22194326 MS.gene062590:CDS 30.0%
ATTGTGTGTTTCTTCATTCT+TGG + chr2.1:22195784-22195803 MS.gene062590:intron 30.0%
CACTACCAAAAGATCATTTA+GGG - chr2.1:22196750-22196769 None:intergenic 30.0%
GAAATATAGTTCGAAAAAGG+AGG - chr2.1:22196284-22196303 None:intergenic 30.0%
TATACTAAGCTTGAGAATGT+AGG + chr2.1:22194479-22194498 MS.gene062590:CDS 30.0%
TATAGACCTGAAGAATCTAT+TGG + chr2.1:22194347-22194366 MS.gene062590:CDS 30.0%
TATCGAAGAAGCAAAGAAAT+TGG + chr2.1:22195472-22195491 MS.gene062590:CDS 30.0%
TCAATCTTTATCGTTCTTCT+AGG - chr2.1:22194899-22194918 None:intergenic 30.0%
TGAGAAGTCATATGCTATAT+TGG + chr2.1:22195351-22195370 MS.gene062590:intron 30.0%
TGGTGATCTGATAAAATAAG+GGG + chr2.1:22196838-22196857 MS.gene062590:intron 30.0%
TTATGTCAAACAACTCAAAC+AGG - chr2.1:22196402-22196421 None:intergenic 30.0%
! AAAGACCCTAAATGATCTTT+TGG + chr2.1:22196742-22196761 MS.gene062590:intron 30.0%
! AACAATTTGAAGCTTTGGTT+TGG + chr2.1:22196818-22196837 MS.gene062590:intron 30.0%
! ATCCGTTTTATCTTTTACCT+TGG + chr2.1:22196233-22196252 MS.gene062590:intron 30.0%
! ATTGTTTGAAATGAAACTGC+TGG + chr2.1:22195137-22195156 MS.gene062590:intron 30.0%
! CGGATAGAGTTTAAAACAAA+AGG - chr2.1:22196218-22196237 None:intergenic 30.0%
! TATCTGATGGCATAAGTTTT+GGG + chr2.1:22196485-22196504 MS.gene062590:intron 30.0%
! TCATTTTGTAGGTTTCTTGT+AGG + chr2.1:22195423-22195442 MS.gene062590:intron 30.0%
! TTATCTGATGGCATAAGTTT+TGG + chr2.1:22196484-22196503 MS.gene062590:intron 30.0%
!! AAGATCATTTAGGGTCTTTT+CGG - chr2.1:22196741-22196760 None:intergenic 30.0%
AAAAAACTCACACAGCAACA+CGG - chr2.1:22194565-22194584 None:intergenic 35.0%
ACTTTAACTGAACAAGCAGA+AGG - chr2.1:22195014-22195033 None:intergenic 35.0%
ACTTTATCAAACATCTCCCT+AGG - chr2.1:22194935-22194954 None:intergenic 35.0%
AGAAGATCGAGCAAGATAAT+AGG + chr2.1:22194294-22194313 MS.gene062590:CDS 35.0%
ATCAAGCACAAGAATTACCA+AGG - chr2.1:22196253-22196272 None:intergenic 35.0%
CCACTACCAAAAGATCATTT+AGG - chr2.1:22196751-22196770 None:intergenic 35.0%
CTCAAACAGGTTATCAATAG+CGG - chr2.1:22196389-22196408 None:intergenic 35.0%
CTTTATCAAACATCTCCCTA+GGG - chr2.1:22194934-22194953 None:intergenic 35.0%
GAGCACTTTGAACATCAAAA+TGG - chr2.1:22194418-22194437 None:intergenic 35.0%
TCAAACAGGTTATCAATAGC+GGG - chr2.1:22196388-22196407 None:intergenic 35.0%
TTTCTTCATTCTTGGTACCA+TGG + chr2.1:22195792-22195811 MS.gene062590:intron 35.0%
TTTGAGTATAAAGCTGCTAC+TGG + chr2.1:22194524-22194543 MS.gene062590:CDS 35.0%
! AAAGTGCTCACACTATTGAA+GGG + chr2.1:22194429-22194448 MS.gene062590:CDS 35.0%
! AGGTACACACTTCATTTTGT+AGG + chr2.1:22195412-22195431 MS.gene062590:intron 35.0%
! CCTAAATGATCTTTTGGTAG+TGG + chr2.1:22196748-22196767 MS.gene062590:intron 35.0%
! GCTCAAAGTCAAGATCTTTT+AGG - chr2.1:22195188-22195207 None:intergenic 35.0%
!! GTTCTGAGGATTACTATTGT+AGG + chr2.1:22194501-22194520 MS.gene062590:CDS 35.0%
!! TATGAGGCATTCTTTTGCTT+TGG + chr2.1:22195626-22195645 MS.gene062590:intron 35.0%
!! TTGCTTTAGCCTTTCGTTTT+TGG + chr2.1:22194584-22194603 MS.gene062590:intron 35.0%
!!! ATAAGTTTTGGGAGTCTGTT+TGG + chr2.1:22196496-22196515 MS.gene062590:intron 35.0%
!!! TAAGTTTTGGGAGTCTGTTT+GGG + chr2.1:22196497-22196516 MS.gene062590:intron 35.0%
AAGACAGTTTCATGGAGCTA+AGG + chr2.1:22194376-22194395 MS.gene062590:CDS 40.0%
AATGCTCTACTACTGAGCTA+TGG + chr2.1:22196190-22196209 MS.gene062590:intron 40.0%
AATGCTGGAAATAGGGAACA+TGG + chr2.1:22195044-22195063 MS.gene062590:intron 40.0%
AGACCTGAAGAATCTATTGG+TGG + chr2.1:22194350-22194369 MS.gene062590:CDS 40.0%
ATAACATATATGGGCCCAAC+CGG + chr2.1:22196136-22196155 MS.gene062590:intron 40.0%
ATGCTGGAAATAGGGAACAT+GGG + chr2.1:22195045-22195064 MS.gene062590:intron 40.0%
ATTAGGTCATCCGGGTTATA+AGG - chr2.1:22194719-22194738 None:intergenic 40.0%
CGATGATTTGAAGAAACCAC+AGG + chr2.1:22195526-22195545 MS.gene062590:CDS 40.0%
CTTCCACCAATAGATTCTTC+AGG - chr2.1:22194356-22194375 None:intergenic 40.0%
GAACAATGCTTGAATTCTGC+AGG + chr2.1:22197105-22197124 MS.gene062590:intron 40.0%
TGAACAAAGACTTAGCCCTA+GGG + chr2.1:22194916-22194935 MS.gene062590:intron 40.0%
TGATTTGAAGAAACCACAGG+CGG + chr2.1:22195529-22195548 MS.gene062590:CDS 40.0%
TTAATATCTAGGGGCCTGTT+TGG + chr2.1:22196443-22196462 MS.gene062590:intron 40.0%
TTATAACCCGGATGACCTAA+TGG + chr2.1:22194718-22194737 MS.gene062590:CDS 40.0%
TTGAACAAAGACTTAGCCCT+AGG + chr2.1:22194915-22194934 MS.gene062590:intron 40.0%
TTTGCTTTGGCCCTTTGATA+CGG + chr2.1:22195639-22195658 MS.gene062590:intron 40.0%
! CAAAGTGCTCACACTATTGA+AGG + chr2.1:22194428-22194447 MS.gene062590:CDS 40.0%
! TTTATCGTTCTTCTAGGAGC+TGG - chr2.1:22194893-22194912 None:intergenic 40.0%
!! AAAACGGATTACGCTAACCA+TGG - chr2.1:22195812-22195831 None:intergenic 40.0%
ACCTAATGGTGCAGTGTGAA+GGG + chr2.1:22194732-22194751 MS.gene062590:CDS 45.0%
ATCACCAGGATCTGATAGCA+AGG + chr2.1:22195562-22195581 MS.gene062590:CDS 45.0%
ATGCGAGATGCCTTATAACC+CGG + chr2.1:22194706-22194725 MS.gene062590:CDS 45.0%
CAATGCTTGAATTCTGCAGG+TGG + chr2.1:22197108-22197127 MS.gene062590:intron 45.0%
CTATCAGATCCTGGTGATAC+AGG - chr2.1:22195560-22195579 None:intergenic 45.0%
CTGTGATAGCCCGTATCAAA+GGG - chr2.1:22195652-22195671 None:intergenic 45.0%
CTGTTATTGGAACTCTGTCC+CGG + chr2.1:22194976-22194995 MS.gene062590:intron 45.0%
GACTGCAGATCAAAAGTCAC+CGG - chr2.1:22196169-22196188 None:intergenic 45.0%
GCTTGAGAATGTAGGTTCTG+AGG + chr2.1:22194487-22194506 MS.gene062590:CDS 45.0%
GGTGGAAGAAGACAGTTTCA+TGG + chr2.1:22194368-22194387 MS.gene062590:CDS 45.0%
TAACATATATGGGCCCAACC+GGG + chr2.1:22196137-22196156 MS.gene062590:intron 45.0%
TCTGTGATAGCCCGTATCAA+AGG - chr2.1:22195653-22195672 None:intergenic 45.0%
TTGCTTTGGCCCTTTGATAC+GGG + chr2.1:22195640-22195659 MS.gene062590:intron 45.0%
ACCCTTCACACTGCACCATT+AGG - chr2.1:22194736-22194755 None:intergenic 50.0%
ACTGCACCATTAGGTCATCC+GGG - chr2.1:22194727-22194746 None:intergenic 50.0%
AGGTACCATCCTGCTTGTGT+GGG + chr2.1:22195443-22195462 MS.gene062590:intron 50.0%
ATAGTCATGCCCACACAAGC+AGG - chr2.1:22195455-22195474 None:intergenic 50.0%
ATCTAGGGGCCTGTTTGGAT+TGG + chr2.1:22196448-22196467 MS.gene062590:intron 50.0%
ATGTGAGGGTTCGTGTGAGA+TGG + chr2.1:22194321-22194340 MS.gene062590:CDS 50.0%
CACTGCACCATTAGGTCATC+CGG - chr2.1:22194728-22194747 None:intergenic 50.0%
GACCTAATGGTGCAGTGTGA+AGG + chr2.1:22194731-22194750 MS.gene062590:CDS 50.0%
TAGGTACCATCCTGCTTGTG+TGG + chr2.1:22195442-22195461 MS.gene062590:intron 50.0%
TTCTCCACACGTGCCACATA+GGG - chr2.1:22194278-22194297 None:intergenic 50.0%
! AGTGTGAAGGGTGCAAGGAT+TGG + chr2.1:22194744-22194763 MS.gene062590:CDS 50.0%
! CAAGCAGAAGGCTTTGTCCT+AGG - chr2.1:22195002-22195021 None:intergenic 50.0%
! CTCACCTTGCTATCAGATCC+TGG - chr2.1:22195569-22195588 None:intergenic 50.0%
!! AAAAGTCACCGGTTCGAACC+CGG - chr2.1:22196158-22196177 None:intergenic 50.0%
ACTCACACAGCAACACGGTC+AGG - chr2.1:22194560-22194579 None:intergenic 55.0%
CACGAGTAAGCCACCCTATG+TGG + chr2.1:22194262-22194281 MS.gene062590:CDS 55.0%
CTTCTCCACACGTGCCACAT+AGG - chr2.1:22194279-22194298 None:intergenic 55.0%
GGTGGCTTACTCGTGTCAGA+AGG - chr2.1:22194257-22194276 None:intergenic 55.0%
TCATGCCCACACAAGCAGGA+TGG - chr2.1:22195451-22195470 None:intergenic 55.0%
! AGAAGGCTTTGTCCTAGGCC+GGG - chr2.1:22194997-22195016 None:intergenic 55.0%
! CAGAAGGCTTTGTCCTAGGC+CGG - chr2.1:22194998-22195017 None:intergenic 55.0%
GATACAGGAAACGCCGCCTG+TGG - chr2.1:22195545-22195564 None:intergenic 60.0%
GCGGCGTTTCCTGTATCACC+AGG + chr2.1:22195548-22195567 MS.gene062590:CDS 60.0%
GGTCCTCTCTTTCGCCGCTT+CGG - chr2.1:22197136-22197155 None:intergenic 60.0%
TCCACACGTGCCACATAGGG+TGG - chr2.1:22194275-22194294 None:intergenic 60.0%
TTCTGCAGGTGGAGCCGAAG+CGG + chr2.1:22197119-22197138 MS.gene062590:intron 60.0%
TTGGAACTCTGTCCCGGCCT+AGG + chr2.1:22194982-22195001 MS.gene062590:intron 60.0%
! GGTGCAGTGTGAAGGGTGCA+AGG + chr2.1:22194739-22194758 MS.gene062590:CDS 60.0%
!! TCACCGGTTCGAACCCGGTT+GGG - chr2.1:22196153-22196172 None:intergenic 60.0%
GAGCCGAAGCGGCGAAAGAG+AGG + chr2.1:22197130-22197149 MS.gene062590:CDS 65.0%
GCCACCCTATGTGGCACGTG+TGG + chr2.1:22194271-22194290 MS.gene062590:CDS 65.0%
!! GTCACCGGTTCGAACCCGGT+TGG - chr2.1:22196154-22196173 None:intergenic 65.0%
GGGCCCAACCGGGTTCGAAC+CGG + chr2.1:22196147-22196166 MS.gene062590:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 22194248 22197159 22194248 ID=MS.gene062590
chr2.1 mRNA 22194248 22197159 22194248 ID=MS.gene062590.t1;Parent=MS.gene062590
chr2.1 exon 22194248 22194573 22194248 ID=MS.gene062590.t1.exon1;Parent=MS.gene062590.t1
chr2.1 CDS 22194248 22194573 22194248 ID=cds.MS.gene062590.t1;Parent=MS.gene062590.t1
chr2.1 exon 22194697 22194765 22194697 ID=MS.gene062590.t1.exon2;Parent=MS.gene062590.t1
chr2.1 CDS 22194697 22194765 22194697 ID=cds.MS.gene062590.t1;Parent=MS.gene062590.t1
chr2.1 exon 22195445 22195583 22195445 ID=MS.gene062590.t1.exon3;Parent=MS.gene062590.t1
chr2.1 CDS 22195445 22195583 22195445 ID=cds.MS.gene062590.t1;Parent=MS.gene062590.t1
chr2.1 exon 22197127 22197159 22197127 ID=MS.gene062590.t1.exon4;Parent=MS.gene062590.t1
chr2.1 CDS 22197127 22197159 22197127 ID=cds.MS.gene062590.t1;Parent=MS.gene062590.t1
Gene Sequence

>MS.gene062590

ATGCGTCCTTCTGACACGAGTAAGCCACCCTATGTGGCACGTGTGGAGAAGATCGAGCAAGATAATAGGAACAATGTGAGGGTTCGTGTGAGATGGTATTATAGACCTGAAGAATCTATTGGTGGAAGAAGACAGTTTCATGGAGCTAAGGAGCTGTTTTTATCTGACCATTTTGATGTTCAAAGTGCTCACACTATTGAAGGGAAATGCATTGTTCACTCTTTCAAGAACTATACTAAGCTTGAGAATGTAGGTTCTGAGGATTACTATTGTAGGTTTGAGTATAAAGCTGCTACTGGTGCTTTCACTCCTGACCGTGTTGCTGTGTGAGTTTTTTTGCTTTAGCCTTTCGTTTTTGGTTCTTTATTTTCCTAATTGAGTTTTGAGTATTTGATTTGATGAGGTTTGTTTTTTGAATAATTTGCTGATTTGGTTTTTGTGTTTTGTAGATACTGCAAATGCGAGATGCCTTATAACCCGGATGACCTAATGGTGCAGTGTGAAGGGTGCAAGGATTGGTAAAATCAGTGTATTCTGTGTTTGTTTAGTTTGAGTGATTTTTAGTTTTTTCCGATATCTAATTTTAGCATTTGGATGTGTTGATTGTTTTGATGTTTTGATGAATGAATATATGTGGATATTCCAGCTCCTAGAAGAACGATAAAGATTGAACAAAGACTTAGCCCTAGGGAGATGTTTGATAAAGTTCAATAGTTGTAATACATTCTCTGTTATTGGAACTCTGTCCCGGCCTAGGACAAAGCCTTCTGCTTGTTCAGTTAAAGTAAAACAGATAAATGCTGGAAATAGGGAACATGGGTGTTTAAAAGATTTTCCGTTAATACAAGAGCTAAAATTATAAATAACAATCAACCTCCAATTGTTTTTCATTGTTTGAAATGAAACTGCTGGTGTCTTTATATCTTCTGTTTGTTGACCTAAAAGATCTTGACTTTGAGCTTAATTGTTTATTTTTTTCCAATCTATAGGTCATAAATTGAATTATGCAAGGGTTTATTTGTCATGCTTTCATCAAATCTTGCTGAACTTATTTAGCATAATGCTTAAATCATCGCAAAAGATTATTGAATTTTTGGAACATCTGAGAAGTCATATGCTATATTGGCTTTTTAGGCGTATGTATTTCCCTCTATATTTTATGAAAGGTACACACTTCATTTTGTAGGTTTCTTGTAGGTACCATCCTGCTTGTGTGGGCATGACTATCGAAGAAGCAAAGAAATTGGACCATTTTGTTTGTTCTGAATGTTCATCTGACGATGATTTGAAGAAACCACAGGCGGCGTTTCCTGTATCACCAGGATCTGATAGCAAGGTGAGATAAATCAGATAACTACATGAATTTAGTTCTAAATATTATGAGGCATTCTTTTGCTTTGGCCCTTTGATACGGGCTATCACAGAATGCTGCTTCATGTGTAAAAAATGAATGTTCGAGTTTTGAACTGATTCATGTATTCTAATTAAAATTTGAGTTTTGAAATGATTCATATATTCTAATTAAAATTTGGTTGAATTGTGTGTTTCTTCATTCTTGGTACCATGGTTAGCGTAATCCGTTTTTTTCTCAAAATTCTTTTTCGTTTTTACTGAAATATGCTACAAAGTGTAATTAGTTGAAGACCTAATGCTGCAATATGATTAAAAGGGAATTATACATTTTATTGCTTAGAAACACCTTTATAAATTTTAAATGTGGTTTTGATATCTATGGTTATGTTTGTGTTAGCTCACGTCTCGAGCTTTGATGTACTCTTTTTAAAAAAAGCTTATGCAAATGTATTGACATCAGAAATTATCTAGGCTTAAAAAAATATGAAATGATTTTTTTTTGTGATTTTCAGAGATTAAAAAGAATTGAAAAAAAATAACATATATGGGCCCAACCGGGTTCGAACCGGTGACTTTTGATCTGCAGTCAAATGCTCTACTACTGAGCTATGGACCCTTTTGTTTTAAACTCTATCCGTTTTATCTTTTACCTTGGTAATTCTTGTGCTTGATGAACTTATCCTCCTTTTTCGAACTATATTTCACAAAATATGTGTACTTTATTCTGTCTATTTTCATAAGATTTAATAAGCCCGTAAATTTTTGTAAATTTGATTTTATCGTGCCCGCTATTGATAACCTGTTTGAGTTGTTTGACATAAGTTGTGACTTGTAATCAGTAGTTAATATCTAGGGGCCTGTTTGGATTGGCTTATATTTAAGCTTATCTGATGGCATAAGTTTTGGGAGTCTGTTTGGGAGAACTTATGAAAATAGCTTACAACAATTTTCATAAGCTTTTCTCAACTTATTTTCTTTAAGTTCTCCAATATTGCTTATAAAAACAACTTATAGCGTATACAGAACAATTTAACCTTTTTTTTTTATCTTTTTCTATAAAAATAGTTTATGCAAGCTTATACATAAGCGCTTATAATGATAAGTGCTTGTGCTATAAGCTGCAAATAAGTTGTTTATCCGAAAAGACCCTAAATGATCTTTTGGTAGTGGTTTTTCTTTCAAAGTGTTTTTCAAAGCTAATTATGTAATTGAATTCTAACAATTTGAAGCTTTGGTTTGGTGATCTGATAAAATAAGGGGCTGTCTTTGCATACAACATATTGTATTTGTGTTTGTTAAAATTGTTGCCTTTATTTTCTTGTAAAAGCCATTAAGCAGTGTTCATTTTGTGAGTAATAAATAATGGAGGAGATTTTAGTCAAGTTTTTTATTCCTTTTTGGACATAATCTCTTTTAATCAATGGTATGTTTGAGTGTTAAGATTTTAATTATTTTGATTGACACTATAGGGTTGTGTGATTGCAAACATGTTTTCATTGATTATGAACAATGCTTGAATTCTGCAGGTGGAGCCGAAGCGGCGAAAGAGAGGACCATGA
Protein sequence

>MS.gene062590.t1

MRPSDTSKPPYVARVEKIEQDNRNNVRVRVRWYYRPEESIGGRRQFHGAKELFLSDHFDVQSAHTIEGKCIVHSFKNYTKLENVGSEDYYCRFEYKAATGAFTPDRVAVYCKCEMPYNPDDLMVQCEGCKDWYHPACVGMTIEEAKKLDHFVCSECSSDDDLKKPQAAFPVSPGSDSKVEPKRRKRGP