Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014380.t1 | XP_003603840.3 | 99.5 | 439 | 2 | 0 | 1 | 439 | 12 | 450 | 4.70E-240 | 840.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014380.t1 | Q5SR56 | 25.8 | 360 | 231 | 8 | 56 | 406 | 81 | 413 | 5.6e-16 | 87.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014380.t1 | G7J5X3 | 99.5 | 434 | 2 | 0 | 6 | 439 | 1 | 434 | 2.0e-237 | 830.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene014380 | MS.gene029256 | PPI |
| MS.gene014380 | MS.gene056267 | PPI |
| MS.gene014380 | MS.gene69359 | PPI |
| MS.gene014380 | MS.gene064986 | PPI |
| MS.gene014380 | MS.gene52801 | PPI |
| MS.gene014380 | MS.gene064798 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014380.t1 | MTR_3g114810 | 99.539 | 434 | 2 | 0 | 6 | 439 | 1 | 434 | 0.0 | 880 |
| MS.gene014380.t1 | MTR_1g112980 | 55.402 | 435 | 183 | 4 | 6 | 438 | 1 | 426 | 5.24e-171 | 487 |
| MS.gene014380.t1 | MTR_1g112980 | 53.804 | 368 | 159 | 4 | 6 | 371 | 1 | 359 | 1.90e-137 | 399 |
| MS.gene014380.t1 | MTR_1g112980 | 53.239 | 355 | 155 | 4 | 6 | 358 | 1 | 346 | 9.86e-130 | 380 |
| MS.gene014380.t1 | MTR_1g092880 | 36.019 | 422 | 253 | 2 | 14 | 432 | 13 | 420 | 1.97e-90 | 281 |
| MS.gene014380.t1 | MTR_1g092870 | 35.800 | 419 | 258 | 2 | 14 | 432 | 13 | 420 | 5.57e-90 | 280 |
| MS.gene014380.t1 | MTR_1g092880 | 36.277 | 419 | 249 | 3 | 14 | 429 | 13 | 416 | 9.21e-89 | 277 |
| MS.gene014380.t1 | MTR_1g092880 | 35.142 | 387 | 234 | 2 | 14 | 397 | 13 | 385 | 1.41e-79 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014380.t1 | AT5G42210 | 57.309 | 431 | 183 | 1 | 7 | 437 | 3 | 432 | 0.0 | 517 |
| MS.gene014380.t1 | AT5G42210 | 56.888 | 392 | 168 | 1 | 7 | 398 | 3 | 393 | 9.70e-164 | 467 |
| MS.gene014380.t1 | AT2G16980 | 44.662 | 459 | 220 | 3 | 3 | 437 | 13 | 461 | 1.67e-138 | 405 |
| MS.gene014380.t1 | AT2G16980 | 44.518 | 456 | 219 | 3 | 6 | 437 | 1 | 446 | 2.88e-137 | 402 |
| MS.gene014380.t1 | AT2G16990 | 48.936 | 423 | 201 | 4 | 13 | 422 | 7 | 427 | 8.43e-137 | 400 |
| MS.gene014380.t1 | AT2G16990 | 48.513 | 437 | 210 | 4 | 13 | 436 | 7 | 441 | 6.25e-136 | 398 |
| MS.gene014380.t1 | AT2G16990 | 48.513 | 437 | 210 | 4 | 13 | 436 | 7 | 441 | 6.25e-136 | 398 |
| MS.gene014380.t1 | AT2G16970 | 45.067 | 446 | 229 | 3 | 6 | 436 | 1 | 445 | 2.16e-135 | 397 |
| MS.gene014380.t1 | AT2G16980 | 44.444 | 459 | 220 | 4 | 3 | 437 | 13 | 460 | 1.08e-133 | 393 |
| MS.gene014380.t1 | AT2G16990 | 48.000 | 400 | 193 | 4 | 13 | 399 | 7 | 404 | 1.24e-124 | 367 |
| MS.gene014380.t1 | AT2G16990 | 48.000 | 400 | 193 | 4 | 13 | 399 | 7 | 404 | 1.24e-124 | 367 |
| MS.gene014380.t1 | AT2G16980 | 43.095 | 420 | 205 | 3 | 3 | 398 | 13 | 422 | 8.45e-120 | 356 |
| MS.gene014380.t1 | AT2G16990 | 45.538 | 437 | 196 | 5 | 13 | 436 | 7 | 414 | 2.72e-119 | 355 |
| MS.gene014380.t1 | AT2G16970 | 43.627 | 408 | 214 | 3 | 6 | 398 | 1 | 407 | 1.77e-117 | 349 |
| MS.gene014380.t1 | AT2G16970 | 43.627 | 408 | 214 | 3 | 6 | 398 | 1 | 407 | 1.77e-117 | 349 |
| MS.gene014380.t1 | AT2G16970 | 42.453 | 424 | 228 | 3 | 6 | 414 | 1 | 423 | 5.02e-117 | 350 |
| MS.gene014380.t1 | AT2G16980 | 41.071 | 448 | 202 | 5 | 3 | 398 | 13 | 450 | 1.92e-115 | 346 |
| MS.gene014380.t1 | AT2G16970 | 43.081 | 383 | 202 | 3 | 6 | 373 | 1 | 382 | 8.00e-106 | 319 |
| MS.gene014380.t1 | AT2G16990 | 46.556 | 363 | 171 | 5 | 13 | 354 | 7 | 367 | 1.62e-103 | 313 |
| MS.gene014380.t1 | AT2G16990 | 46.556 | 363 | 171 | 5 | 13 | 354 | 7 | 367 | 1.62e-103 | 313 |
| MS.gene014380.t1 | AT2G16970 | 41.282 | 390 | 208 | 4 | 6 | 380 | 1 | 384 | 2.22e-101 | 307 |
| MS.gene014380.t1 | AT2G16970 | 43.417 | 357 | 186 | 3 | 6 | 347 | 1 | 356 | 7.87e-100 | 303 |
| MS.gene014380.t1 | AT2G16980 | 41.124 | 338 | 165 | 3 | 123 | 437 | 1 | 327 | 1.40e-81 | 255 |
| MS.gene014380.t1 | AT2G16970 | 54.598 | 174 | 78 | 1 | 6 | 178 | 1 | 174 | 1.57e-57 | 193 |
| MS.gene014380.t1 | AT2G16970 | 49.390 | 164 | 83 | 0 | 273 | 436 | 175 | 338 | 4.08e-51 | 176 |
Find 93 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCGACTTAATGATCATTTC+TGG | 0.173185 | 3.2:+90757449 | MS.gene014380:CDS |
| TTGTGTGCAGTATTACTTAA+AGG | 0.198024 | 3.2:+90757397 | MS.gene014380:intron |
| CTTGCAGATGTTGGCCTTCA+TGG | 0.200217 | 3.2:+90757277 | MS.gene014380:CDS |
| ACTTTCCACTCACCCGTTAA+TGG | 0.208512 | 3.2:-90758538 | None:intergenic |
| GCTTGTGATGATGCCCATTT+TGG | 0.228414 | 3.2:+90754879 | MS.gene014380:CDS |
| ATCAGAGAGATCACCCAAAA+TGG | 0.242550 | 3.2:-90754893 | None:intergenic |
| GCACTGCTTCTTCGACCTTC+TGG | 0.250970 | 3.2:-90756332 | None:intergenic |
| GGTGATCTCTCTGATAAGTA+TGG | 0.311306 | 3.2:+90754901 | MS.gene014380:CDS |
| GGCTTTCCTTGTGAAATGAT+TGG | 0.315353 | 3.2:-90756628 | None:intergenic |
| CTGAAGCCAGGAAAATTAAA+AGG | 0.317779 | 3.2:-90758655 | None:intergenic |
| ATGATCATTTCTGGTATTGC+AGG | 0.320953 | 3.2:+90757458 | MS.gene014380:CDS |
| GCTTTCCTTGTGAAATGATT+GGG | 0.321252 | 3.2:-90756627 | None:intergenic |
| GGGATAATCATGAGGGTCAT+AGG | 0.323838 | 3.2:-90754946 | None:intergenic |
| ATGGAGAAATTGAATATGTA+TGG | 0.326739 | 3.2:+90749405 | MS.gene014380:CDS |
| AATCATGAGTGGGAGAATTA+TGG | 0.327479 | 3.2:+90749386 | None:intergenic |
| CTTGGCTTTCTCTCCATTAA+CGG | 0.331892 | 3.2:+90758525 | MS.gene014380:CDS |
| TCATAATTTATCGACATTCA+TGG | 0.337515 | 3.2:+90749458 | MS.gene014380:CDS |
| AGTGCATTTGGAGTTCTTTC+AGG | 0.349547 | 3.2:+90756350 | MS.gene014380:CDS |
| GGTCGAAGAAGCAGTGCATT+TGG | 0.349597 | 3.2:+90756338 | MS.gene014380:CDS |
| TATGTCCATCTAACTCAAAA+TGG | 0.361400 | 3.2:-90758833 | None:intergenic |
| AGGGCTCAAGGTTGCATCTC+AGG | 0.406266 | 3.2:+90758472 | MS.gene014380:CDS |
| TTGGCTTTCTCTCCATTAAC+GGG | 0.411542 | 3.2:+90758526 | MS.gene014380:CDS |
| AAGTGAGCATTCATCTTGTC+CGG | 0.414098 | 3.2:-90749523 | None:intergenic |
| ACAGCATAGCATGGTCCTCT+TGG | 0.414980 | 3.2:+90757810 | MS.gene014380:CDS |
| AAAGGAGGTTATATCTCTTA+AGG | 0.419562 | 3.2:-90756713 | None:intergenic |
| TTCAGCAACCTTGCAGATGT+TGG | 0.424383 | 3.2:+90757268 | MS.gene014380:CDS |
| GACCCTCATGATTATCCCCT+TGG | 0.424391 | 3.2:+90754951 | MS.gene014380:CDS |
| GGCTTCAGTATAATGTGTAT+TGG | 0.431166 | 3.2:+90758670 | MS.gene014380:CDS |
| TGCACATATTGTATCTCCCT+TGG | 0.435532 | 3.2:+90758507 | MS.gene014380:CDS |
| AGCTCTATTTCTATCTGAAA+AGG | 0.445760 | 3.2:+90758630 | MS.gene014380:intron |
| GAGAACATTGCAGCAGCATA+AGG | 0.448056 | 3.2:-90758003 | None:intergenic |
| CAGCATAGCATGGTCCTCTT+GGG | 0.448466 | 3.2:+90757811 | MS.gene014380:CDS |
| TGGATGATTCGCAGGCATAT+TGG | 0.454868 | 3.2:+90756046 | MS.gene014380:intron |
| TTTGGAGTTCTTTCAGGGAT+AGG | 0.457164 | 3.2:+90756356 | MS.gene014380:CDS |
| AAATGATTGGGGTATAAAGT+TGG | 0.469648 | 3.2:-90756615 | None:intergenic |
| CTCCTACTTTGGGCGAGGCA+AGG | 0.477680 | 3.2:+90757651 | MS.gene014380:CDS |
| TTCTTTCAGGGAAGGGCTCA+AGG | 0.479698 | 3.2:+90758460 | MS.gene014380:intron |
| TTGAAAACCAGTGAGATAGA+TGG | 0.487866 | 3.2:-90749547 | None:intergenic |
| TGCAGTATTACTTAAAGGCT+CGG | 0.491711 | 3.2:+90757402 | MS.gene014380:intron |
| ATTGTTACATCGGTGATTGC+TGG | 0.492648 | 3.2:-90749486 | None:intergenic |
| GTGCATTTGGAGTTCTTTCA+GGG | 0.492657 | 3.2:+90756351 | MS.gene014380:CDS |
| ATCATTAACATGATTTGCGA+TGG | 0.498843 | 3.2:+90756119 | MS.gene014380:CDS |
| GAGGCAAGGCTTCTTTCGAT+TGG | 0.501981 | 3.2:+90757665 | MS.gene014380:CDS |
| TCACTTGCCATCTATCTCAC+TGG | 0.503898 | 3.2:+90749540 | MS.gene014380:CDS |
| TATTGCAGGGACTGTGTCAC+AGG | 0.508069 | 3.2:+90757472 | MS.gene014380:CDS |
| TAACCTCCTTTCTCAATAGT+AGG | 0.508494 | 3.2:+90756725 | MS.gene014380:CDS |
| AATGTGTATTGGAATTGCTT+CGG | 0.510199 | 3.2:+90758681 | MS.gene014380:CDS |
| GAGCTCAATGCTTTCAAAAG+TGG | 0.510207 | 3.2:-90756691 | None:intergenic |
| ACCCTCATGATTATCCCCTT+GGG | 0.512456 | 3.2:+90754952 | MS.gene014380:CDS |
| GGATAATCATGAGGGTCATA+GGG | 0.513412 | 3.2:-90754945 | None:intergenic |
| TCAGAGAGATCACCCAAAAT+GGG | 0.513615 | 3.2:-90754892 | None:intergenic |
| GGTCATAGGGAGCGTAAGAA+TGG | 0.513622 | 3.2:-90754932 | None:intergenic |
| CTTTCCTTGTGAAATGATTG+GGG | 0.515488 | 3.2:-90756626 | None:intergenic |
| ACAAGTTGATCCCCGTGAGC+AGG | 0.529631 | 3.2:+90758183 | MS.gene014380:CDS |
| CTCTGATAAGTATGGGAGGA+AGG | 0.534368 | 3.2:+90754909 | MS.gene014380:CDS |
| TTAACATGATACCTGCTCAC+GGG | 0.541820 | 3.2:-90758194 | None:intergenic |
| GTCACACCTACTATTGAGAA+AGG | 0.547596 | 3.2:-90756731 | None:intergenic |
| TGATCATTTCTGGTATTGCA+GGG | 0.548846 | 3.2:+90757459 | MS.gene014380:CDS |
| ACAATGGCTGCTCTTTGTCC+CGG | 0.553213 | 3.2:+90749504 | MS.gene014380:CDS |
| ACCAGAAATGATCATTAAGT+CGG | 0.565383 | 3.2:-90757450 | None:intergenic |
| GAAAGACGGTCATGAAGAGA+TGG | 0.572645 | 3.2:-90749434 | None:intergenic |
| TCCATCTAACTCAAAATGGA+AGG | 0.576928 | 3.2:-90758829 | None:intergenic |
| TGGCCTTCATGGTTCAATGA+TGG | 0.579807 | 3.2:+90757288 | MS.gene014380:CDS |
| AGTGAGCATTCATCTTGTCC+GGG | 0.580465 | 3.2:-90749522 | None:intergenic |
| GTGATCTCTCTGATAAGTAT+GGG | 0.589147 | 3.2:+90754902 | MS.gene014380:CDS |
| ATACCATCATTGAACCATGA+AGG | 0.590082 | 3.2:-90757291 | None:intergenic |
| AGCCTTGCCTCGCCCAAAGT+AGG | 0.594097 | 3.2:-90757653 | None:intergenic |
| TCAGAGTATGATGCTTAGAG+TGG | 0.595054 | 3.2:+90758804 | MS.gene014380:CDS |
| ATACACATTATACTGAAGCC+AGG | 0.605025 | 3.2:-90758667 | None:intergenic |
| ATCTCTCTGATAAGTATGGG+AGG | 0.608663 | 3.2:+90754905 | MS.gene014380:CDS |
| ATTAACATGATACCTGCTCA+CGG | 0.609111 | 3.2:-90758195 | None:intergenic |
| TGTTCATTTACAGCATAGCA+TGG | 0.610699 | 3.2:+90757801 | MS.gene014380:CDS |
| TACCCAAGGGGATAATCATG+AGG | 0.613909 | 3.2:-90754954 | None:intergenic |
| CGATAAATTATGAAGAAAGA+CGG | 0.614156 | 3.2:-90749448 | None:intergenic |
| ATTAACATATAATCATGAGT+GGG | 0.615387 | 3.2:+90749376 | None:intergenic |
| TGGAGAAATTGAATATGTAT+GGG | 0.616448 | 3.2:+90749406 | MS.gene014380:CDS |
| AATTAACATATAATCATGAG+TGG | 0.618145 | 3.2:+90749375 | None:intergenic |
| TATACCCCAATCATTTCACA+AGG | 0.622314 | 3.2:+90756622 | MS.gene014380:CDS |
| AGCAATCACCGATGTAACAA+TGG | 0.634236 | 3.2:+90749488 | MS.gene014380:CDS |
| AAGAGCAGCCATTGTTACAT+CGG | 0.640036 | 3.2:-90749496 | None:intergenic |
| TAACATGATACCTGCTCACG+GGG | 0.648157 | 3.2:-90758193 | None:intergenic |
| CGTTAATGGAGAGAAAGCCA+AGG | 0.651353 | 3.2:-90758524 | None:intergenic |
| ACCCAAGGGGATAATCATGA+GGG | 0.653332 | 3.2:-90754953 | None:intergenic |
| TAGGCAGATAACGTTCCAGA+AGG | 0.656602 | 3.2:+90756317 | MS.gene014380:intron |
| CATGAAGGCCAACATCTGCA+AGG | 0.659193 | 3.2:-90757276 | None:intergenic |
| CACCTGAAAAGTTTGAGCAG+CGG | 0.670212 | 3.2:-90756423 | None:intergenic |
| CATCGGCTACTGAATCCCGA+AGG | 0.670653 | 3.2:-90756588 | None:intergenic |
| ACGTAAGCTAGAGCGAGACA+AGG | 0.675262 | 3.2:-90756149 | None:intergenic |
| GTTAATGGAGAGAAAGCCAA+GGG | 0.684337 | 3.2:-90758523 | None:intergenic |
| ACACCTACTATTGAGAAAGG+AGG | 0.685291 | 3.2:-90756728 | None:intergenic |
| CAGCAAAAGACATACCCAAG+AGG | 0.697205 | 3.2:-90757825 | None:intergenic |
| TGTTGCACACACAAACGCTG+AGG | 0.726307 | 3.2:-90756381 | None:intergenic |
| AGTAGAAAGAAGTACCCAAG+GGG | 0.729323 | 3.2:-90754966 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 90749390 | 90758846 | 90749390 | ID=MS.gene014380 |
| chr3.2 | mRNA | 90749390 | 90758846 | 90749390 | ID=MS.gene014380.t1;Parent=MS.gene014380 |
| chr3.2 | exon | 90749390 | 90749575 | 90749390 | ID=MS.gene014380.t1.exon1;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90749390 | 90749575 | 90749390 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90754862 | 90754973 | 90754862 | ID=MS.gene014380.t1.exon2;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90754862 | 90754973 | 90754862 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90756060 | 90756172 | 90756060 | ID=MS.gene014380.t1.exon3;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90756060 | 90756172 | 90756060 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90756320 | 90756442 | 90756320 | ID=MS.gene014380.t1.exon4;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90756320 | 90756442 | 90756320 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90756541 | 90756746 | 90756541 | ID=MS.gene014380.t1.exon5;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90756541 | 90756746 | 90756541 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90757234 | 90757309 | 90757234 | ID=MS.gene014380.t1.exon6;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90757234 | 90757309 | 90757234 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90757407 | 90757493 | 90757407 | ID=MS.gene014380.t1.exon7;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90757407 | 90757493 | 90757407 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90757629 | 90757709 | 90757629 | ID=MS.gene014380.t1.exon8;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90757629 | 90757709 | 90757629 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90757800 | 90757832 | 90757800 | ID=MS.gene014380.t1.exon9;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90757800 | 90757832 | 90757800 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90758000 | 90758053 | 90758000 | ID=MS.gene014380.t1.exon10;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90758000 | 90758053 | 90758000 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90758163 | 90758204 | 90758163 | ID=MS.gene014380.t1.exon11;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90758163 | 90758204 | 90758163 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90758469 | 90758547 | 90758469 | ID=MS.gene014380.t1.exon12;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90758469 | 90758547 | 90758469 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90758632 | 90758702 | 90758632 | ID=MS.gene014380.t1.exon13;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90758632 | 90758702 | 90758632 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| chr3.2 | exon | 90758790 | 90758846 | 90758790 | ID=MS.gene014380.t1.exon14;Parent=MS.gene014380.t1 |
| chr3.2 | CDS | 90758790 | 90758846 | 90758790 | ID=cds.MS.gene014380.t1;Parent=MS.gene014380.t1 |
| Gene Sequence |
| Protein sequence |
