AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene014514

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014514.t1 MTR_3g111890 93.784 370 21 1 1 368 1 370 0.0 662
MS.gene014514.t1 MTR_3g111890 93.784 370 21 1 1 368 1 370 0.0 661
MS.gene014514.t1 MTR_5g020300 55.399 213 93 1 111 321 59 271 5.72e-86 262
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014514.t1 AT1G19480 61.798 356 115 6 15 368 41 377 3.46e-146 419
MS.gene014514.t1 AT1G19480 61.798 356 115 6 15 368 41 377 5.23e-146 418
MS.gene014514.t1 AT1G75230 61.053 380 114 5 6 368 24 386 8.29e-146 418
MS.gene014514.t1 AT1G75230 61.053 380 114 5 6 368 24 386 8.51e-146 418
MS.gene014514.t1 AT3G50880 54.152 277 90 4 46 320 29 270 1.85e-99 296

Find 113 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCAACAGCTTCGTCAAAT+TGG 0.169969 3.1:+89329359 MS.gene014514:CDS
TGGTGGAATTGGTTGGCTTT+AGG 0.180405 3.1:-89328998 None:intergenic
CTTCGTCAAATTGGGGTTTC+TGG 0.192533 3.1:+89329368 MS.gene014514:CDS
TGATTCCGCTATTGTAAATA+TGG 0.193076 3.1:+89329448 MS.gene014514:CDS
TATGAATAACATGTTTAATA+TGG 0.206711 3.1:+89329844 MS.gene014514:CDS
ACTATGCTTACTATGGTTAA+TGG 0.221809 3.1:+89329488 MS.gene014514:CDS
GGTGGAATTGGTTGGCTTTA+GGG 0.224070 3.1:-89328997 None:intergenic
CTCAACAGCTTCGTCAAATT+GGG 0.256135 3.1:+89329360 MS.gene014514:CDS
GCTGCTGTTGCCACTGGCAA+TGG 0.292196 3.1:+89329743 MS.gene014514:CDS
AGCTAGTTATCTTCATGATT+TGG 0.297314 3.1:+89329397 MS.gene014514:CDS
TTATTCATAGGATCCATAAT+TGG 0.299949 3.1:-89329830 None:intergenic
CTATGCTTACTATGGTTAAT+GGG 0.309757 3.1:+89329489 MS.gene014514:CDS
TGGTTAATGGGATTGGTTCT+TGG 0.310260 3.1:+89329501 MS.gene014514:CDS
GCATTCACAACAACCAATTA+TGG 0.336085 3.1:+89329817 MS.gene014514:CDS
TTGGGTCGGGTGAGACCTTT+CGG 0.350316 3.1:-89328950 None:intergenic
ATATTAAACATGTTATTCAT+AGG 0.364793 3.1:-89329842 None:intergenic
CTTATCGTTCGGTTGCTTCA+TGG 0.372094 3.1:+89329675 MS.gene014514:CDS
AGCAATAAACCGGGTATAGA+TGG 0.372851 3.1:-89329285 None:intergenic
ATGAATAACATGTTTAATAT+GGG 0.373528 3.1:+89329845 MS.gene014514:CDS
CTTACTATGGTTAATGGGAT+TGG 0.385045 3.1:+89329494 MS.gene014514:CDS
ATTGAGATTCGGAGGTTGTT+GGG 0.390972 3.1:-89328968 None:intergenic
GGTACAAGATACTACGGGTT+AGG 0.395255 3.1:-89329235 None:intergenic
GATTCGGAGGTTGTTGGGTC+GGG 0.407111 3.1:-89328963 None:intergenic
TGGCTTGCAGCAGCATGAGC+TGG 0.409325 3.1:+89329763 MS.gene014514:CDS
CGAACACCAAGATCATTAAT+CGG 0.413843 3.1:-89329572 None:intergenic
GTTAGGGCAAGAACATTATC+AGG 0.416123 3.1:-89329335 None:intergenic
GACGAAGCTGTTGAGCAGTT+AGG 0.418751 3.1:-89329352 None:intergenic
ACCCCTTGTGGTTGTGGCTG+AGG 0.426942 3.1:-89328789 None:intergenic
ACGAAGCTGTTGAGCAGTTA+GGG 0.427388 3.1:-89329351 None:intergenic
CGATTAATGATCTTGGTGTT+CGG 0.428190 3.1:+89329573 MS.gene014514:CDS
GTTCTTCCGATTAATGATCT+TGG 0.429181 3.1:+89329566 MS.gene014514:CDS
ATGATCTTGGTGTTCGGAAA+GGG 0.431899 3.1:+89329579 MS.gene014514:CDS
TAGGGTTTCCGATTGAGATT+CGG 0.437075 3.1:-89328979 None:intergenic
ACTGCTGCTGCTGTTGCCAC+TGG 0.440661 3.1:+89329737 MS.gene014514:CDS
ATTGCTCAGTTCGGATTCTA+CGG 0.445751 3.1:-89328871 None:intergenic
CCGGCGGTGGTGGAATTGGT+TGG 0.451771 3.1:-89329005 None:intergenic
GCTCTATGCGGCGGTGAAGC+TGG 0.455065 3.1:+89329305 MS.gene014514:CDS
TCATTAATCGGAAGAACATC+AGG 0.455995 3.1:-89329560 None:intergenic
CGGCTTTAAAAGCGAGTTGT+TGG 0.456722 3.1:-89329256 None:intergenic
TTAGGGCAAGGAATGGAGTC+TGG 0.460253 3.1:-89329217 None:intergenic
TGATGGAACATTGCTCAGTT+CGG 0.471327 3.1:-89328880 None:intergenic
AGATTCGGAGGTTGTTGGGT+CGG 0.473762 3.1:-89328964 None:intergenic
GAAGAAACCCCTTGTGGTTG+TGG 0.474078 3.1:-89328795 None:intergenic
GATTGAGATTCGGAGGTTGT+TGG 0.484783 3.1:-89328969 None:intergenic
AACAACCTCCGAATCTCAAT+CGG 0.488511 3.1:+89328971 MS.gene014514:CDS
CGGTTGCTTCATGGTATATG+TGG 0.491099 3.1:+89329684 MS.gene014514:CDS
GACGGAGTCGACAGCGGTGG+TGG 0.492448 3.1:-89328835 None:intergenic
CGCCGCATAGAGCAATAAAC+CGG 0.498184 3.1:-89329295 None:intergenic
GTGGAGGTTTGTTGAAGCGA+AGG 0.503950 3.1:+89329703 MS.gene014514:CDS
GAAGTGGAAGCCTTATCGTT+CGG 0.505451 3.1:+89329664 MS.gene014514:CDS
GCTGCTGCTGCTGTTGATGG+TGG 0.507705 3.1:-89329787 None:intergenic
TGGAGGTTTGTTGAAGCGAA+GGG 0.516701 3.1:+89329704 MS.gene014514:CDS
CTTGCCTCGGCCATCTCAGA+TGG 0.518209 3.1:+89329628 MS.gene014514:CDS
ATCATCCATATTTACAATAG+CGG 0.520609 3.1:-89329453 None:intergenic
ACAATTGAGGTTCGTTGTTG+TGG 0.522531 3.1:-89329065 None:intergenic
TTGCTCAGTTCGGATTCTAC+GGG 0.522751 3.1:-89328870 None:intergenic
GTTATCTTCATGATTTGGCT+AGG 0.524825 3.1:+89329402 MS.gene014514:CDS
GCAACAGCAGCAGCAGTTGA+AGG 0.531886 3.1:-89329731 None:intergenic
GGAGTCGACAGCGGTGGTGG+CGG 0.536044 3.1:-89328832 None:intergenic
AATGATCTTGGTGTTCGGAA+AGG 0.537519 3.1:+89329578 MS.gene014514:CDS
CATGAAGCAACCGAACGATA+AGG 0.537784 3.1:-89329674 None:intergenic
GTGCGAAGGTGAAGTTGAGA+TGG 0.543548 3.1:+89329115 MS.gene014514:CDS
TGGCTAGGAAGTATCAGAAT+GGG 0.545890 3.1:+89329417 MS.gene014514:CDS
GTACAAGATACTACGGGTTA+GGG 0.549172 3.1:-89329234 None:intergenic
GGTGGCGGAGATTGTGTTGT+CGG 0.551572 3.1:-89328817 None:intergenic
GTTGTTGGTACAAGATACTA+CGG 0.551894 3.1:-89329241 None:intergenic
TTGCTTCATGGTATATGTGG+AGG 0.557460 3.1:+89329687 MS.gene014514:CDS
TCGGATCTTACGAGGACGGA+GGG 0.560273 3.1:-89328931 None:intergenic
TTGGCTAGGAAGTATCAGAA+TGG 0.561670 3.1:+89329416 MS.gene014514:CDS
TTGTTCCGAAAATTGTAGCT+AGG 0.562008 3.1:+89329084 MS.gene014514:CDS
ATAATCTAGATGACTTGCCT+CGG 0.562183 3.1:+89329615 MS.gene014514:CDS
TCAACAGCTTCGTCAAATTG+GGG 0.564716 3.1:+89329361 MS.gene014514:CDS
CCGGGTATAGATGGAAGTAC+CGG 0.564853 3.1:-89329276 None:intergenic
TGATCTTGGTGTTCGGAAAG+GGG 0.566268 3.1:+89329580 MS.gene014514:CDS
CCGGTACTTCCATCTATACC+CGG 0.576685 3.1:+89329276 MS.gene014514:CDS
TGGATCAATTATGTGAGAAG+TGG 0.576910 3.1:+89329648 MS.gene014514:CDS
CATAATTGATCCATCTGAGA+TGG 0.576912 3.1:-89329638 None:intergenic
CTACGGGTTAGGGCAAGGAA+TGG 0.578814 3.1:-89329224 None:intergenic
CAACGGACGGAACAACAAAA+CGG 0.582839 3.1:+89329034 MS.gene014514:CDS
ATTGAGGTTCGTTGTTGTGG+TGG 0.584929 3.1:-89329062 None:intergenic
GCTAGGTCACTTTCGTGCGA+AGG 0.586188 3.1:+89329101 MS.gene014514:CDS
AGATACTACGGGTTAGGGCA+AGG 0.587198 3.1:-89329229 None:intergenic
CCAACCAATTCCACCACCGC+CGG 0.587677 3.1:+89329005 MS.gene014514:CDS
TACGGGGATAATAGTTTCGA+CGG 0.589338 3.1:-89328853 None:intergenic
CCGCCGGAAAATCCAACGGA+CGG 0.590648 3.1:+89329021 MS.gene014514:CDS
CGATGAGAGGTGATAAGAGT+GGG 0.592687 3.1:-89329163 None:intergenic
CAACCTCAGCCACAACCACA+AGG 0.593857 3.1:+89328786 MS.gene014514:CDS
TGAATAACATGTTTAATATG+GGG 0.599833 3.1:+89329846 MS.gene014514:CDS
GGTTTCCGATTGAGATTCGG+AGG 0.602513 3.1:-89328976 None:intergenic
ACCTCAGCCACAACCACAAG+GGG 0.604739 3.1:+89328788 MS.gene014514:CDS
TGATCCATCTGAGATGGCCG+AGG 0.610828 3.1:-89329632 None:intergenic
CTGCTGCAAGCCATTGCCAG+TGG 0.628730 3.1:-89329753 None:intergenic
GAGACCTTTCGGATCTTACG+AGG 0.632398 3.1:-89328939 None:intergenic
AGAGTGGGTCTGCGGTACGA+AGG 0.640787 3.1:-89329148 None:intergenic
TTCGGATCTTACGAGGACGG+AGG 0.648070 3.1:-89328932 None:intergenic
CGGTTTATTGCTCTATGCGG+CGG 0.648731 3.1:+89329296 MS.gene014514:CDS
TTGTTGGTACAAGATACTAC+GGG 0.649339 3.1:-89329240 None:intergenic
AACCTCAGCCACAACCACAA+GGG 0.649650 3.1:+89328787 MS.gene014514:CDS
AGGTGATAAGAGTGGGTCTG+CGG 0.654092 3.1:-89329156 None:intergenic
AGTTTCGACGGAGTCGACAG+CGG 0.661441 3.1:-89328841 None:intergenic
CCGTCCTCGTAAGATCCGAA+AGG 0.662065 3.1:+89328935 MS.gene014514:CDS
ACCACCGCCGGAAAATCCAA+CGG 0.663130 3.1:+89329017 MS.gene014514:CDS
CCTTTCGGATCTTACGAGGA+CGG 0.664581 3.1:-89328935 None:intergenic
TTCGACGGAGTCGACAGCGG+TGG 0.669382 3.1:-89328838 None:intergenic
TGGAAATTATCAAACGTAGG+TGG 0.669577 3.1:-89329197 None:intergenic
TGCTCAGTTCGGATTCTACG+GGG 0.671223 3.1:-89328869 None:intergenic
TCGATGAGAGGTGATAAGAG+TGG 0.675819 3.1:-89329164 None:intergenic
ACCCGGTTTATTGCTCTATG+CGG 0.679051 3.1:+89329293 MS.gene014514:CDS
CGGATCTTACGAGGACGGAG+GGG 0.706604 3.1:-89328930 None:intergenic
GGTTGATGAATGTCGATGAG+AGG 0.708425 3.1:-89329176 None:intergenic
TTGTCGGAAGAAACCCCTTG+TGG 0.713634 3.1:-89328801 None:intergenic
GCCGCATAGAGCAATAAACC+GGG 0.722126 3.1:-89329294 None:intergenic
GTCTGGAAATTATCAAACGT+AGG 0.725407 3.1:-89329200 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAAACATGTTATTCAT+AGG - chr3.1:89329845-89329864 None:intergenic 15.0%
!! ATGAATAACATGTTTAATAT+GGG + chr3.1:89329845-89329864 MS.gene014514:CDS 15.0%
!! TATGAATAACATGTTTAATA+TGG + chr3.1:89329844-89329863 MS.gene014514:CDS 15.0%
!! TGAATAACATGTTTAATATG+GGG + chr3.1:89329846-89329865 MS.gene014514:CDS 20.0%
! ATCATCCATATTTACAATAG+CGG - chr3.1:89329456-89329475 None:intergenic 25.0%
! TTATTCATAGGATCCATAAT+TGG - chr3.1:89329833-89329852 None:intergenic 25.0%
ACTATGCTTACTATGGTTAA+TGG + chr3.1:89329488-89329507 MS.gene014514:CDS 30.0%
AGCTAGTTATCTTCATGATT+TGG + chr3.1:89329397-89329416 MS.gene014514:CDS 30.0%
CTATGCTTACTATGGTTAAT+GGG + chr3.1:89329489-89329508 MS.gene014514:CDS 30.0%
TGATTCCGCTATTGTAAATA+TGG + chr3.1:89329448-89329467 MS.gene014514:CDS 30.0%
! TACAATTTTCGGAACAATTG+AGG - chr3.1:89329081-89329100 None:intergenic 30.0%
CATAATTGATCCATCTGAGA+TGG - chr3.1:89329641-89329660 None:intergenic 35.0%
CGAACACCAAGATCATTAAT+CGG - chr3.1:89329575-89329594 None:intergenic 35.0%
CTTACTATGGTTAATGGGAT+TGG + chr3.1:89329494-89329513 MS.gene014514:CDS 35.0%
GCATTCACAACAACCAATTA+TGG + chr3.1:89329817-89329836 MS.gene014514:CDS 35.0%
GTCTGGAAATTATCAAACGT+AGG - chr3.1:89329203-89329222 None:intergenic 35.0%
GTTCTTCCGATTAATGATCT+TGG + chr3.1:89329566-89329585 MS.gene014514:CDS 35.0%
GTTGTTGGTACAAGATACTA+CGG - chr3.1:89329244-89329263 None:intergenic 35.0%
TCATTAATCGGAAGAACATC+AGG - chr3.1:89329563-89329582 None:intergenic 35.0%
TGGAAATTATCAAACGTAGG+TGG - chr3.1:89329200-89329219 None:intergenic 35.0%
TGGATCAATTATGTGAGAAG+TGG + chr3.1:89329648-89329667 MS.gene014514:CDS 35.0%
TTGTTCCGAAAATTGTAGCT+AGG + chr3.1:89329084-89329103 MS.gene014514:CDS 35.0%
TTGTTGGTACAAGATACTAC+GGG - chr3.1:89329243-89329262 None:intergenic 35.0%
! AGTGACCTAGCTACAATTTT+CGG - chr3.1:89329092-89329111 None:intergenic 35.0%
! ATAATCTAGATGACTTGCCT+CGG + chr3.1:89329615-89329634 MS.gene014514:CDS 35.0%
! GCTTTTCACTATGCTTACTA+TGG + chr3.1:89329481-89329500 MS.gene014514:CDS 35.0%
! GTTATCTTCATGATTTGGCT+AGG + chr3.1:89329402-89329421 MS.gene014514:CDS 35.0%
!! CGATTAATGATCTTGGTGTT+CGG + chr3.1:89329573-89329592 MS.gene014514:CDS 35.0%
AACAACCTCCGAATCTCAAT+CGG + chr3.1:89328971-89328990 MS.gene014514:CDS 40.0%
ACAATTGAGGTTCGTTGTTG+TGG - chr3.1:89329068-89329087 None:intergenic 40.0%
AGCAATAAACCGGGTATAGA+TGG - chr3.1:89329288-89329307 None:intergenic 40.0%
ATTGAGATTCGGAGGTTGTT+GGG - chr3.1:89328971-89328990 None:intergenic 40.0%
ATTGCTCAGTTCGGATTCTA+CGG - chr3.1:89328874-89328893 None:intergenic 40.0%
CTCAACAGCTTCGTCAAATT+GGG + chr3.1:89329360-89329379 MS.gene014514:CDS 40.0%
GTTAGGGCAAGAACATTATC+AGG - chr3.1:89329338-89329357 None:intergenic 40.0%
TACGGGGATAATAGTTTCGA+CGG - chr3.1:89328856-89328875 None:intergenic 40.0%
TAGGGTTTCCGATTGAGATT+CGG - chr3.1:89328982-89329001 None:intergenic 40.0%
TCAACAGCTTCGTCAAATTG+GGG + chr3.1:89329361-89329380 MS.gene014514:CDS 40.0%
TGATGGAACATTGCTCAGTT+CGG - chr3.1:89328883-89328902 None:intergenic 40.0%
TTGCTTCATGGTATATGTGG+AGG + chr3.1:89329687-89329706 MS.gene014514:CDS 40.0%
! GTACAAGATACTACGGGTTA+GGG - chr3.1:89329237-89329256 None:intergenic 40.0%
!! AATGATCTTGGTGTTCGGAA+AGG + chr3.1:89329578-89329597 MS.gene014514:CDS 40.0%
!! ATGATCTTGGTGTTCGGAAA+GGG + chr3.1:89329579-89329598 MS.gene014514:CDS 40.0%
!! TGGCTAGGAAGTATCAGAAT+GGG + chr3.1:89329417-89329436 MS.gene014514:CDS 40.0%
!! TGGTTAATGGGATTGGTTCT+TGG + chr3.1:89329501-89329520 MS.gene014514:CDS 40.0%
!! TTGGCTAGGAAGTATCAGAA+TGG + chr3.1:89329416-89329435 MS.gene014514:CDS 40.0%
!!! CAACAACTCGCTTTTAAAGC+CGG + chr3.1:89329257-89329276 MS.gene014514:CDS 40.0%
ACCCGGTTTATTGCTCTATG+CGG + chr3.1:89329293-89329312 MS.gene014514:CDS 45.0%
ACGAAGCTGTTGAGCAGTTA+GGG - chr3.1:89329354-89329373 None:intergenic 45.0%
ATTGAGGTTCGTTGTTGTGG+TGG - chr3.1:89329065-89329084 None:intergenic 45.0%
CAACGGACGGAACAACAAAA+CGG + chr3.1:89329034-89329053 MS.gene014514:CDS 45.0%
CATGAAGCAACCGAACGATA+AGG - chr3.1:89329677-89329696 None:intergenic 45.0%
CGATGAGAGGTGATAAGAGT+GGG - chr3.1:89329166-89329185 None:intergenic 45.0%
CGGTTGCTTCATGGTATATG+TGG + chr3.1:89329684-89329703 MS.gene014514:CDS 45.0%
CTTATCGTTCGGTTGCTTCA+TGG + chr3.1:89329675-89329694 MS.gene014514:CDS 45.0%
CTTCGTCAAATTGGGGTTTC+TGG + chr3.1:89329368-89329387 MS.gene014514:CDS 45.0%
GATTGAGATTCGGAGGTTGT+TGG - chr3.1:89328972-89328991 None:intergenic 45.0%
GCTCAACAGCTTCGTCAAAT+TGG + chr3.1:89329359-89329378 MS.gene014514:CDS 45.0%
GGTACAAGATACTACGGGTT+AGG - chr3.1:89329238-89329257 None:intergenic 45.0%
TCGATGAGAGGTGATAAGAG+TGG - chr3.1:89329167-89329186 None:intergenic 45.0%
TTGCTCAGTTCGGATTCTAC+GGG - chr3.1:89328873-89328892 None:intergenic 45.0%
! GAAGTGGAAGCCTTATCGTT+CGG + chr3.1:89329664-89329683 MS.gene014514:CDS 45.0%
!! CGGCTTTAAAAGCGAGTTGT+TGG - chr3.1:89329259-89329278 None:intergenic 45.0%
!! GGTGGAATTGGTTGGCTTTA+GGG - chr3.1:89329000-89329019 None:intergenic 45.0%
!! GGTTGATGAATGTCGATGAG+AGG - chr3.1:89329179-89329198 None:intergenic 45.0%
!! TGATCTTGGTGTTCGGAAAG+GGG + chr3.1:89329580-89329599 MS.gene014514:CDS 45.0%
!! TGGAGGTTTGTTGAAGCGAA+GGG + chr3.1:89329704-89329723 MS.gene014514:CDS 45.0%
!! TGGTGGAATTGGTTGGCTTT+AGG - chr3.1:89329001-89329020 None:intergenic 45.0%
AACCTCAGCCACAACCACAA+GGG + chr3.1:89328787-89328806 MS.gene014514:CDS 50.0%
AGATTCGGAGGTTGTTGGGT+CGG - chr3.1:89328967-89328986 None:intergenic 50.0%
AGGTGATAAGAGTGGGTCTG+CGG - chr3.1:89329159-89329178 None:intergenic 50.0%
CCGGTACTTCCATCTATACC+CGG + chr3.1:89329276-89329295 MS.gene014514:CDS 50.0%
CCTTTCGGATCTTACGAGGA+CGG - chr3.1:89328938-89328957 None:intergenic 50.0%
CGCCGCATAGAGCAATAAAC+CGG - chr3.1:89329298-89329317 None:intergenic 50.0%
CGGTTTATTGCTCTATGCGG+CGG + chr3.1:89329296-89329315 MS.gene014514:CDS 50.0%
GAAGAAACCCCTTGTGGTTG+TGG - chr3.1:89328798-89328817 None:intergenic 50.0%
GACGAAGCTGTTGAGCAGTT+AGG - chr3.1:89329355-89329374 None:intergenic 50.0%
GAGACCTTTCGGATCTTACG+AGG - chr3.1:89328942-89328961 None:intergenic 50.0%
GCCGCATAGAGCAATAAACC+GGG - chr3.1:89329297-89329316 None:intergenic 50.0%
GGTTTCCGATTGAGATTCGG+AGG - chr3.1:89328979-89328998 None:intergenic 50.0%
GTGCGAAGGTGAAGTTGAGA+TGG + chr3.1:89329115-89329134 MS.gene014514:CDS 50.0%
TGCTCAGTTCGGATTCTACG+GGG - chr3.1:89328872-89328891 None:intergenic 50.0%
TTAGGGCAAGGAATGGAGTC+TGG - chr3.1:89329220-89329239 None:intergenic 50.0%
TTGTCGGAAGAAACCCCTTG+TGG - chr3.1:89328804-89328823 None:intergenic 50.0%
! CCGGGTATAGATGGAAGTAC+CGG - chr3.1:89329279-89329298 None:intergenic 50.0%
! GTTCCGTCCGTTGGATTTTC+CGG - chr3.1:89329027-89329046 None:intergenic 50.0%
!! AGATACTACGGGTTAGGGCA+AGG - chr3.1:89329232-89329251 None:intergenic 50.0%
!! GTGGAGGTTTGTTGAAGCGA+AGG + chr3.1:89329703-89329722 MS.gene014514:CDS 50.0%
!!! CGTTTTGTTGTTCCGTCCGT+TGG - chr3.1:89329036-89329055 None:intergenic 50.0%
ACCACCGCCGGAAAATCCAA+CGG + chr3.1:89329017-89329036 MS.gene014514:CDS 55.0%
ACCTCAGCCACAACCACAAG+GGG + chr3.1:89328788-89328807 MS.gene014514:CDS 55.0%
AGTTTCGACGGAGTCGACAG+CGG - chr3.1:89328844-89328863 None:intergenic 55.0%
CAACCTCAGCCACAACCACA+AGG + chr3.1:89328786-89328805 MS.gene014514:CDS 55.0%
CCGTCCTCGTAAGATCCGAA+AGG + chr3.1:89328935-89328954 MS.gene014514:CDS 55.0%
GATTCGGAGGTTGTTGGGTC+GGG - chr3.1:89328966-89328985 None:intergenic 55.0%
GCAACAGCAGCAGCAGTTGA+AGG - chr3.1:89329734-89329753 None:intergenic 55.0%
TCGGATCTTACGAGGACGGA+GGG - chr3.1:89328934-89328953 None:intergenic 55.0%
TTCGGATCTTACGAGGACGG+AGG - chr3.1:89328935-89328954 None:intergenic 55.0%
TTGGGTCGGGTGAGACCTTT+CGG - chr3.1:89328953-89328972 None:intergenic 55.0%
! GCTAGGTCACTTTCGTGCGA+AGG + chr3.1:89329101-89329120 MS.gene014514:CDS 55.0%
! TGATCCATCTGAGATGGCCG+AGG - chr3.1:89329635-89329654 None:intergenic 55.0%
!! CTACGGGTTAGGGCAAGGAA+TGG - chr3.1:89329227-89329246 None:intergenic 55.0%
!! GGTGGCGGAGATTGTGTTGT+CGG - chr3.1:89328820-89328839 None:intergenic 55.0%
!!! ACGGAGGGGCATTTTTGTGG+CGG - chr3.1:89328919-89328938 None:intergenic 55.0%
!!! AGGACGGAGGGGCATTTTTG+TGG - chr3.1:89328922-89328941 None:intergenic 55.0%
!!! TTTTCCGGCGGTGGTGGAAT+TGG - chr3.1:89329012-89329031 None:intergenic 55.0%
ACCCCTTGTGGTTGTGGCTG+AGG - chr3.1:89328792-89328811 None:intergenic 60.0%
ACTGCTGCTGCTGTTGCCAC+TGG + chr3.1:89329737-89329756 MS.gene014514:CDS 60.0%
AGAGTGGGTCTGCGGTACGA+AGG - chr3.1:89329151-89329170 None:intergenic 60.0%
CCAACCAATTCCACCACCGC+CGG + chr3.1:89329005-89329024 MS.gene014514:CDS 60.0%
CCGCCGGAAAATCCAACGGA+CGG + chr3.1:89329021-89329040 MS.gene014514:CDS 60.0%
CGGATCTTACGAGGACGGAG+GGG - chr3.1:89328933-89328952 None:intergenic 60.0%
CTGCTGCAAGCCATTGCCAG+TGG - chr3.1:89329756-89329775 None:intergenic 60.0%
CTTGCCTCGGCCATCTCAGA+TGG + chr3.1:89329628-89329647 MS.gene014514:CDS 60.0%
GCTGCTGCTGCTGCTGTTGA+TGG - chr3.1:89329793-89329812 None:intergenic 60.0%
GCTGCTGTTGCCACTGGCAA+TGG + chr3.1:89329743-89329762 MS.gene014514:CDS 60.0%
! CCGTCCGTTGGATTTTCCGG+CGG - chr3.1:89329024-89329043 None:intergenic 60.0%
! GCTGCTGCTGCTGTTGATGG+TGG - chr3.1:89329790-89329809 None:intergenic 60.0%
! GTTGGATTTTCCGGCGGTGG+TGG - chr3.1:89329018-89329037 None:intergenic 60.0%
! TCCGTTGGATTTTCCGGCGG+TGG - chr3.1:89329021-89329040 None:intergenic 60.0%
! TGGCTTGCAGCAGCATGAGC+TGG + chr3.1:89329763-89329782 MS.gene014514:CDS 60.0%
!! CATTTTTGTGGCGGGGGCGT+TGG - chr3.1:89328910-89328929 None:intergenic 60.0%
!!! CGGAGGGGCATTTTTGTGGC+GGG - chr3.1:89328918-89328937 None:intergenic 60.0%
!!! GAGGGGCATTTTTGTGGCGG+GGG - chr3.1:89328916-89328935 None:intergenic 60.0%
!!! GGAGGGGCATTTTTGTGGCG+GGG - chr3.1:89328917-89328936 None:intergenic 60.0%
GCTCTATGCGGCGGTGAAGC+TGG + chr3.1:89329305-89329324 MS.gene014514:CDS 65.0%
TTCGACGGAGTCGACAGCGG+TGG - chr3.1:89328841-89328860 None:intergenic 65.0%
!! CCGGCGGTGGTGGAATTGGT+TGG - chr3.1:89329008-89329027 None:intergenic 65.0%
GACGGAGTCGACAGCGGTGG+TGG - chr3.1:89328838-89328857 None:intergenic 70.0%
! GGAGTCGACAGCGGTGGTGG+CGG - chr3.1:89328835-89328854 None:intergenic 70.0%
!! GCGGGGGCGTTGGTGCGTGA+TGG - chr3.1:89328900-89328919 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 89328765 89329871 89328765 ID=MS.gene014514
chr3.1 mRNA 89328765 89329871 89328765 ID=MS.gene014514.t1;Parent=MS.gene014514
chr3.1 exon 89328765 89329871 89328765 ID=MS.gene014514.t1.exon1;Parent=MS.gene014514.t1
chr3.1 CDS 89328765 89329871 89328765 ID=cds.MS.gene014514.t1;Parent=MS.gene014514.t1
Gene Sequence

>MS.gene014514

ATGGGCGAAGACACACAACAACAACCTCAGCCACAACCACAAGGGGTTTCTTCCGACAACACAATCTCCGCCACCACCGCTGTCGACTCCGTCGAAACTATTATCCCCGTAGAATCCGAACTGAGCAATGTTCCATCACGCACCAACGCCCCCGCCACAAAAATGCCCCTCCGTCCTCGTAAGATCCGAAAGGTCTCACCCGACCCAACAACCTCCGAATCTCAATCGGAAACCCTAAAGCCAACCAATTCCACCACCGCCGGAAAATCCAACGGACGGAACAACAAAACGGTTCAACCACCACAACAACGAACCTCAATTGTTCCGAAAATTGTAGCTAGGTCACTTTCGTGCGAAGGTGAAGTTGAGATGGCAATTCGTTACCTTCGTACCGCAGACCCACTCTTATCACCTCTCATCGACATTCATCAACCACCTACGTTTGATAATTTCCAGACTCCATTCCTTGCCCTAACCCGTAGTATCTTGTACCAACAACTCGCTTTTAAAGCCGGTACTTCCATCTATACCCGGTTTATTGCTCTATGCGGCGGTGAAGCTGGTGTTGTTCCTGATAATGTTCTTGCCCTAACTGCTCAACAGCTTCGTCAAATTGGGGTTTCTGGTAGAAAAGCTAGTTATCTTCATGATTTGGCTAGGAAGTATCAGAATGGGATACTTTCTGATTCCGCTATTGTAAATATGGATGATAAATCGCTTTTCACTATGCTTACTATGGTTAATGGGATTGGTTCTTGGTCTGTTCATATGTTTATGATTTTCTCGCTGCATAGACCTGATGTTCTTCCGATTAATGATCTTGGTGTTCGGAAAGGGGTTCAGATTCTTTATAATCTAGATGACTTGCCTCGGCCATCTCAGATGGATCAATTATGTGAGAAGTGGAAGCCTTATCGTTCGGTTGCTTCATGGTATATGTGGAGGTTTGTTGAAGCGAAGGGAAGTCCTTCAACTGCTGCTGCTGTTGCCACTGGCAATGGCTTGCAGCAGCATGAGCTGGACCACCATCAACAGCAGCAGCAGCAGCAGCAGCATTCACAACAACCAATTATGGATCCTATGAATAACATGTTTAATATGGGGTAA
Protein sequence

>MS.gene014514.t1

MGEDTQQQPQPQPQGVSSDNTISATTAVDSVETIIPVESELSNVPSRTNAPATKMPLRPRKIRKVSPDPTTSESQSETLKPTNSTTAGKSNGRNNKTVQPPQQRTSIVPKIVARSLSCEGEVEMAIRYLRTADPLLSPLIDIHQPPTFDNFQTPFLALTRSILYQQLAFKAGTSIYTRFIALCGGEAGVVPDNVLALTAQQLRQIGVSGRKASYLHDLARKYQNGILSDSAIVNMDDKSLFTMLTMVNGIGSWSVHMFMIFSLHRPDVLPINDLGVRKGVQILYNLDDLPRPSQMDQLCEKWKPYRSVASWYMWRFVEAKGSPSTAAAVATGNGLQQHELDHHQQQQQQQQHSQQPIMDPMNNMFNMG