Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89656.t1 | XP_003629318.1 | 95.6 | 613 | 27 | 0 | 1 | 613 | 1 | 613 | 0.00E+00 | 1179.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89656.t1 | F4JNY0 | 54.7 | 611 | 231 | 12 | 1 | 590 | 1 | 586 | 3.9e-177 | 622.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89656.t1 | G7LC44 | 95.6 | 613 | 27 | 0 | 1 | 613 | 1 | 613 | 0.0e+00 | 1179.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene014514 | MS.gene89656 | PPI |
| MS.gene064668 | MS.gene89656 | PPI |
| MS.gene42339 | MS.gene89656 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89656.t1 | MTR_8g075810 | 95.595 | 613 | 27 | 0 | 1 | 613 | 1 | 613 | 0.0 | 1221 |
| MS.gene89656.t1 | MTR_1g051380 | 28.571 | 287 | 150 | 12 | 1 | 279 | 247 | 486 | 7.27e-21 | 96.7 |
| MS.gene89656.t1 | MTR_1g051380 | 28.571 | 287 | 150 | 12 | 1 | 279 | 282 | 521 | 1.19e-20 | 96.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89656.t1 | AT4G36050 | 56.398 | 633 | 232 | 12 | 1 | 613 | 1 | 609 | 0.0 | 669 |
| MS.gene89656.t1 | AT4G36050 | 55.574 | 610 | 227 | 12 | 1 | 590 | 1 | 586 | 0.0 | 628 |
| MS.gene89656.t1 | AT4G36050 | 55.574 | 610 | 227 | 12 | 1 | 590 | 1 | 586 | 0.0 | 627 |
| MS.gene89656.t1 | AT4G36050 | 53.000 | 500 | 193 | 10 | 133 | 613 | 2 | 478 | 8.87e-177 | 510 |
| MS.gene89656.t1 | AT4G36050 | 50.116 | 431 | 173 | 9 | 202 | 613 | 1 | 408 | 3.76e-133 | 396 |
| MS.gene89656.t1 | AT2G41460 | 29.078 | 282 | 143 | 14 | 1 | 273 | 229 | 462 | 2.02e-20 | 95.1 |
| MS.gene89656.t1 | AT2G41460 | 29.078 | 282 | 143 | 14 | 1 | 273 | 269 | 502 | 2.58e-20 | 95.1 |
| MS.gene89656.t1 | AT2G41460 | 29.078 | 282 | 143 | 14 | 1 | 273 | 276 | 509 | 3.60e-20 | 94.7 |
Find 125 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACTGTGGCTACTTTAAA+TGG | 0.152521 | 8.3:+27941856 | MS.gene89656:CDS |
| CCTTACCTCAGTGTGCTTTC+AGG | 0.154312 | 8.3:-27935990 | None:intergenic |
| AAGAAACAAGGAATTAATTT+TGG | 0.195598 | 8.3:+27937227 | MS.gene89656:CDS |
| GAAGAATCGGTTGAGAAAAT+TGG | 0.229830 | 8.3:+27936684 | MS.gene89656:CDS |
| AGGAAAGTCGCCATTCTTTA+TGG | 0.238038 | 8.3:+27934132 | MS.gene89656:CDS |
| TCCAGTTTGTGTGACTTTAA+TGG | 0.258068 | 8.3:+27936403 | MS.gene89656:CDS |
| AACCAGCATTCTTACTTATC+AGG | 0.297547 | 8.3:-27935334 | None:intergenic |
| CAGGAGTCTGTTTCTAAATC+AGG | 0.306871 | 8.3:+27936795 | MS.gene89656:CDS |
| GCAAGATGTTACAGCTGATT+TGG | 0.308409 | 8.3:+27933341 | MS.gene89656:CDS |
| CTATAAAACTGTGGGATTGC+AGG | 0.312579 | 8.3:-27935917 | None:intergenic |
| GGTGCTGAAGTGTTTAACTA+TGG | 0.324478 | 8.3:+27935840 | MS.gene89656:CDS |
| AAGTAGCCACAGTTTGCTTC+AGG | 0.325228 | 8.3:-27941849 | None:intergenic |
| TCCATTAAAGTCACACAAAC+TGG | 0.332934 | 8.3:-27936404 | None:intergenic |
| GCTGATTTGGTTATGGTTGA+TGG | 0.337740 | 8.3:+27933354 | MS.gene89656:CDS |
| TGCACCAGAACTTCCCAAAA+AGG | 0.350483 | 8.3:+27933396 | MS.gene89656:CDS |
| AAAGGTCGAACCGGTTATTC+TGG | 0.351311 | 8.3:+27933414 | MS.gene89656:CDS |
| AGATCAATGTTAATCGAAAA+TGG | 0.352901 | 8.3:+27935277 | MS.gene89656:CDS |
| CTACCTTCTGAATCCTGGTT+TGG | 0.383211 | 8.3:-27936735 | None:intergenic |
| GTTTCAAGTAATTGAGAGTT+GGG | 0.385376 | 8.3:-27936993 | None:intergenic |
| GAACCCGTGGAAAGTTCATT+TGG | 0.391490 | 8.3:-27936768 | None:intergenic |
| AGATATGTTCCCATGGTTCA+TGG | 0.396510 | 8.3:+27936470 | MS.gene89656:CDS |
| GTCTCTTTAATAGAAAAGAT+TGG | 0.415808 | 8.3:+27935043 | MS.gene89656:intron |
| GGAAGGAGAATATTTGTTGT+TGG | 0.420744 | 8.3:+27935083 | MS.gene89656:CDS |
| ACTTTCCACGGGTTCTTCTC+AGG | 0.421646 | 8.3:+27936776 | MS.gene89656:CDS |
| ACCTGAACTTTGCGACCAAC+AGG | 0.421706 | 8.3:-27935820 | None:intergenic |
| TGTTACAGCTGATTTGGTTA+TGG | 0.425965 | 8.3:+27933347 | MS.gene89656:CDS |
| TAACTCAGTTCGGTTCTCTT+CGG | 0.427962 | 8.3:+27933259 | MS.gene89656:CDS |
| GAATGGCGACTTTCCTTCAC+TGG | 0.429430 | 8.3:-27934125 | None:intergenic |
| AAATGCTACCTTCTGAATCC+TGG | 0.431250 | 8.3:-27936740 | None:intergenic |
| ACCTCTTTGCAAGGGACATA+AGG | 0.431309 | 8.3:+27937181 | MS.gene89656:CDS |
| TTCAGGAGACAAAACTTAGT+AGG | 0.431990 | 8.3:+27933319 | MS.gene89656:CDS |
| AAACATCTTACCAGAATAAC+CGG | 0.433256 | 8.3:-27933424 | None:intergenic |
| AATATTTATGGGCCTCGAGC+TGG | 0.436917 | 8.3:+27934471 | MS.gene89656:CDS |
| AAATGATCTAATGGAGAGTT+GGG | 0.438987 | 8.3:-27936889 | None:intergenic |
| ACCTGTTGGTCGCAAAGTTC+AGG | 0.446750 | 8.3:+27935819 | MS.gene89656:CDS |
| TTGTCCTCCCTTCCATCTGT+GGG | 0.452635 | 8.3:-27936351 | None:intergenic |
| TAACGAAGCAGCATTGCCTT+TGG | 0.452671 | 8.3:+27934060 | MS.gene89656:CDS |
| AGTTTCAAGTAATTGAGAGT+TGG | 0.453611 | 8.3:-27936994 | None:intergenic |
| AAAATGATCTAATGGAGAGT+TGG | 0.454041 | 8.3:-27936890 | None:intergenic |
| ACAAAGAATGTCGGCGTCGA+AGG | 0.454652 | 8.3:-27933295 | None:intergenic |
| TTTGATAATAGTAGCAATCC+TGG | 0.455217 | 8.3:+27937026 | MS.gene89656:CDS |
| ATCGACCATGTTCTATGTGC+TGG | 0.462463 | 8.3:+27935870 | MS.gene89656:CDS |
| TTTGTCCTCCCTTCCATCTG+TGG | 0.465273 | 8.3:-27936352 | None:intergenic |
| CAAAGTCTAAGAATAAATAA+TGG | 0.466243 | 8.3:+27941886 | MS.gene89656:CDS |
| TGGTTTGAGAGTTACACAAT+AGG | 0.471042 | 8.3:-27934105 | None:intergenic |
| GCAGCTGAGGAAGGATTCAC+TGG | 0.472111 | 8.3:+27934082 | MS.gene89656:CDS |
| AGCATTGCCTTTGGCAGCTG+AGG | 0.482389 | 8.3:+27934069 | MS.gene89656:CDS |
| ACGCGATTTGTTTCCAAACC+AGG | 0.490337 | 8.3:+27936722 | MS.gene89656:CDS |
| AGACAACGCATAACTCAGTT+CGG | 0.490844 | 8.3:+27933249 | MS.gene89656:CDS |
| ATCTTCTAGATATGTTCCCA+TGG | 0.497181 | 8.3:+27936463 | MS.gene89656:CDS |
| TCACATCGATCAACTGCAAA+TGG | 0.498001 | 8.3:-27935122 | None:intergenic |
| TTTGCTTCAGGATTAGATGC+AGG | 0.498119 | 8.3:-27941837 | None:intergenic |
| TCTCGTGTAGGTTTAGAAGA+TGG | 0.499563 | 8.3:+27935251 | MS.gene89656:intron |
| GAAACCAAATGAACTTTCCA+CGG | 0.500899 | 8.3:+27936764 | MS.gene89656:CDS |
| GGACAAAGCATTAAACTTGA+AGG | 0.501673 | 8.3:+27936368 | MS.gene89656:CDS |
| AATAAATGATAGTTCTAGAA+AGG | 0.510778 | 8.3:+27937094 | MS.gene89656:CDS |
| TGCTGTACGCCATGAACCAT+GGG | 0.511925 | 8.3:-27936479 | None:intergenic |
| TTGTGAGAGAATAGAAGAAT+CGG | 0.514180 | 8.3:+27936671 | MS.gene89656:CDS |
| AAACTGTGGCTACTTTAAAT+GGG | 0.514226 | 8.3:+27941857 | MS.gene89656:CDS |
| CGATGTGTTGTCACTGATCA+TGG | 0.514363 | 8.3:+27934211 | MS.gene89656:CDS |
| AATTGAGAGTTGGGTTGTGA+GGG | 0.515091 | 8.3:-27936984 | None:intergenic |
| TGGAAACAAATCGCGTTCAC+AGG | 0.519942 | 8.3:-27936715 | None:intergenic |
| ACTTATCAGGATGCTTTGCT+CGG | 0.520453 | 8.3:-27935321 | None:intergenic |
| GGGAACATATCTAGAAGATA+AGG | 0.520548 | 8.3:-27936459 | None:intergenic |
| TTGCCTTTGGCAGCTGAGGA+AGG | 0.524451 | 8.3:+27934073 | MS.gene89656:CDS |
| TATTGATTTCTCTAATAATG+AGG | 0.528827 | 8.3:+27936956 | MS.gene89656:CDS |
| AGATCTTCTTCCATAAAGAA+TGG | 0.529753 | 8.3:-27934142 | None:intergenic |
| TTTACCACTCGAGCAATACA+AGG | 0.530202 | 8.3:-27937206 | None:intergenic |
| TTGCTTCAGGATTAGATGCA+GGG | 0.533173 | 8.3:-27941836 | None:intergenic |
| CACACAAACTGGAGCATGAT+CGG | 0.534810 | 8.3:-27936393 | None:intergenic |
| GCAGTTGATCGATGTGATGC+AGG | 0.536666 | 8.3:+27935128 | MS.gene89656:CDS |
| GCATGAACAGTTCCCACAGA+TGG | 0.539768 | 8.3:+27936339 | MS.gene89656:intron |
| GAATGCTGATGTGTGTGGTC+AGG | 0.540883 | 8.3:+27937061 | MS.gene89656:CDS |
| AAATTCTGTCTCAGTACAAA+CGG | 0.544323 | 8.3:+27935961 | MS.gene89656:CDS |
| TAATTGAGAGTTGGGTTGTG+AGG | 0.546654 | 8.3:-27936985 | None:intergenic |
| GTCACTGATCATGGTCACTT+TGG | 0.552191 | 8.3:+27934220 | MS.gene89656:CDS |
| AGTTCTGGTGCAAGAAAAGA+AGG | 0.552315 | 8.3:-27933385 | None:intergenic |
| GAACAGTATACCTCTTTGCA+AGG | 0.553623 | 8.3:+27937172 | MS.gene89656:CDS |
| GAATTTCACATTCCTTAACA+TGG | 0.554845 | 8.3:-27935944 | None:intergenic |
| GTGCTGAAGTGTTTAACTAT+GGG | 0.558176 | 8.3:+27935841 | MS.gene89656:CDS |
| AACAGTTCCCACAGATGGAA+GGG | 0.567253 | 8.3:+27936344 | MS.gene89656:intron |
| CTTTATGGAAGAAGATCTTG+AGG | 0.569766 | 8.3:+27934147 | MS.gene89656:CDS |
| TTGCTGTACGCCATGAACCA+TGG | 0.570004 | 8.3:-27936480 | None:intergenic |
| GCATTCATTTCATCTTGCCC+AGG | 0.570543 | 8.3:-27937044 | None:intergenic |
| GAACAGTTCCCACAGATGGA+AGG | 0.573296 | 8.3:+27936343 | MS.gene89656:intron |
| AACATATCTAGAAGATAAGG+AGG | 0.575747 | 8.3:-27936456 | None:intergenic |
| CATGGCGTACAGCAAACTCT+AGG | 0.575863 | 8.3:+27936488 | MS.gene89656:CDS |
| GACTTTAATGGAAATTCCTG+AGG | 0.580781 | 8.3:+27936415 | MS.gene89656:CDS |
| TTCTCAGTGTTGATAGTGAA+GGG | 0.583392 | 8.3:+27934188 | MS.gene89656:CDS |
| CTATGCTTGTGCTCGTGCTG+AGG | 0.584407 | 8.3:+27937259 | MS.gene89656:CDS |
| TCCTTATGTCCCTTGCAAAG+AGG | 0.586516 | 8.3:-27937182 | None:intergenic |
| GTACTATGCAGAGAGACCTC+AGG | 0.591808 | 8.3:-27936431 | None:intergenic |
| CTCCTGAAAACAAAGAATGT+CGG | 0.592651 | 8.3:-27933304 | None:intergenic |
| CTTCTCAGTGTTGATAGTGA+AGG | 0.593068 | 8.3:+27934187 | MS.gene89656:CDS |
| TGTAACTCTCAAACCAGTGA+AGG | 0.593372 | 8.3:+27934112 | MS.gene89656:CDS |
| ATTTATGGGCCTCGAGCTGG+AGG | 0.597751 | 8.3:+27934474 | MS.gene89656:CDS |
| TTTCCAAACCAGGATTCAGA+AGG | 0.600090 | 8.3:+27936732 | MS.gene89656:CDS |
| AAACCAAATGAACTTTCCAC+GGG | 0.600801 | 8.3:+27936765 | MS.gene89656:CDS |
| GTGTCATCACCTCCAGCTCG+AGG | 0.601324 | 8.3:-27934483 | None:intergenic |
| TTGGAGTCTCTACTGCATCA+AGG | 0.602644 | 8.3:+27935062 | MS.gene89656:CDS |
| ATGGCATCCTATAAAACTGT+GGG | 0.603496 | 8.3:-27935925 | None:intergenic |
| GGAACCTTGTATTGCTCGAG+TGG | 0.604847 | 8.3:+27937202 | MS.gene89656:CDS |
| AGTTTCTGAATCGTGCAAGA+TGG | 0.605400 | 8.3:+27936620 | MS.gene89656:CDS |
| AATCCTTCCTCAGCTGCCAA+AGG | 0.608022 | 8.3:-27934076 | None:intergenic |
| AAGTCTTAACAATGGTGTCA+AGG | 0.611069 | 8.3:+27936926 | MS.gene89656:CDS |
| AGTCTCTACTGCATCAAGGA+AGG | 0.612945 | 8.3:+27935066 | MS.gene89656:CDS |
| GTAATGTAGCTTCACAAGAG+TGG | 0.617764 | 8.3:+27937126 | MS.gene89656:CDS |
| GCTCGAGTGGTAAAGAAACA+AGG | 0.624985 | 8.3:+27937215 | MS.gene89656:CDS |
| TTGATAATAGTAGCAATCCT+GGG | 0.632376 | 8.3:+27937027 | MS.gene89656:CDS |
| ATGAACCAGCACATAGAACA+TGG | 0.633244 | 8.3:-27935875 | None:intergenic |
| CATGGCATCCTATAAAACTG+TGG | 0.641920 | 8.3:-27935926 | None:intergenic |
| ATAGTGAGTTACAACGTGAA+CGG | 0.642885 | 8.3:+27933222 | MS.gene89656:CDS |
| GAATCGTGCAAGATGGCACA+CGG | 0.644414 | 8.3:+27936627 | MS.gene89656:CDS |
| GAATGGATGCATACACCTGT+TGG | 0.658070 | 8.3:+27935805 | MS.gene89656:intron |
| AAAAGCACAAGTCTTAACAA+TGG | 0.660120 | 8.3:+27936918 | MS.gene89656:CDS |
| AACAGTATACCTCTTTGCAA+GGG | 0.660613 | 8.3:+27937173 | MS.gene89656:CDS |
| AGACTCCTGAGAAGAACCCG+TGG | 0.689382 | 8.3:-27936781 | None:intergenic |
| GGCACACGGAGACATTTCAA+CGG | 0.696451 | 8.3:+27936641 | MS.gene89656:CDS |
| AGTTCCCACAGATGGAAGGG+AGG | 0.697066 | 8.3:+27936347 | MS.gene89656:intron |
| ACATATCTAGAAGATAAGGA+GGG | 0.703016 | 8.3:-27936455 | None:intergenic |
| CTGGAGGTGATGACACAGAG+AGG | 0.709721 | 8.3:+27934490 | MS.gene89656:CDS |
| TCTAATCCTGAAGCAAACTG+TGG | 0.715189 | 8.3:+27941843 | MS.gene89656:CDS |
| TCAATGTTAATCGAAAATGG+AGG | 0.725984 | 8.3:+27935280 | MS.gene89656:CDS |
| GAAATGAATGCTGATGTGTG+TGG | 0.774543 | 8.3:+27937056 | MS.gene89656:CDS |
| TCAAACCTGAAAGCACACTG+AGG | 0.790491 | 8.3:+27935985 | MS.gene89656:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 27933216 | 27941905 | 27933216 | ID=MS.gene89656 |
| chr8.3 | mRNA | 27933216 | 27941905 | 27933216 | ID=MS.gene89656.t1;Parent=MS.gene89656 |
| chr8.3 | exon | 27933216 | 27933435 | 27933216 | ID=MS.gene89656.t1.exon1;Parent=MS.gene89656.t1 |
| chr8.3 | CDS | 27933216 | 27933435 | 27933216 | ID=cds.MS.gene89656.t1;Parent=MS.gene89656.t1 |
| chr8.3 | exon | 27934020 | 27934241 | 27934020 | ID=MS.gene89656.t1.exon2;Parent=MS.gene89656.t1 |
| chr8.3 | CDS | 27934020 | 27934241 | 27934020 | ID=cds.MS.gene89656.t1;Parent=MS.gene89656.t1 |
| chr8.3 | exon | 27934463 | 27934548 | 27934463 | ID=MS.gene89656.t1.exon3;Parent=MS.gene89656.t1 |
| chr8.3 | CDS | 27934463 | 27934548 | 27934463 | ID=cds.MS.gene89656.t1;Parent=MS.gene89656.t1 |
| chr8.3 | exon | 27935056 | 27935171 | 27935056 | ID=MS.gene89656.t1.exon4;Parent=MS.gene89656.t1 |
| chr8.3 | CDS | 27935056 | 27935171 | 27935056 | ID=cds.MS.gene89656.t1;Parent=MS.gene89656.t1 |
| chr8.3 | exon | 27935261 | 27935341 | 27935261 | ID=MS.gene89656.t1.exon5;Parent=MS.gene89656.t1 |
| chr8.3 | CDS | 27935261 | 27935341 | 27935261 | ID=cds.MS.gene89656.t1;Parent=MS.gene89656.t1 |
| chr8.3 | exon | 27935806 | 27936006 | 27935806 | ID=MS.gene89656.t1.exon6;Parent=MS.gene89656.t1 |
| chr8.3 | CDS | 27935806 | 27936006 | 27935806 | ID=cds.MS.gene89656.t1;Parent=MS.gene89656.t1 |
| chr8.3 | exon | 27936349 | 27936509 | 27936349 | ID=MS.gene89656.t1.exon7;Parent=MS.gene89656.t1 |
| chr8.3 | CDS | 27936349 | 27936509 | 27936349 | ID=cds.MS.gene89656.t1;Parent=MS.gene89656.t1 |
| chr8.3 | exon | 27936598 | 27937280 | 27936598 | ID=MS.gene89656.t1.exon8;Parent=MS.gene89656.t1 |
| chr8.3 | CDS | 27936598 | 27937280 | 27936598 | ID=cds.MS.gene89656.t1;Parent=MS.gene89656.t1 |
| chr8.3 | exon | 27941834 | 27941905 | 27941834 | ID=MS.gene89656.t1.exon9;Parent=MS.gene89656.t1 |
| chr8.3 | CDS | 27941834 | 27941905 | 27941834 | ID=cds.MS.gene89656.t1;Parent=MS.gene89656.t1 |
| Gene Sequence |
| Protein sequence |
