Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016686.t1 | KEH19074.1 | 94.7 | 532 | 26 | 2 | 1 | 531 | 1 | 531 | 2.30E-265 | 924.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016686.t1 | Q9SYD6 | 57.4 | 526 | 197 | 6 | 3 | 527 | 16 | 515 | 4.2e-143 | 509.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016686.t1 | A0A072TNI6 | 94.7 | 532 | 26 | 2 | 1 | 531 | 1 | 531 | 1.6e-265 | 924.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene31105 | MS.gene016686 | PPI |
| MS.gene45542 | MS.gene016686 | PPI |
| MS.gene016686 | MS.gene052066 | PPI |
| MS.gene78396 | MS.gene016686 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016686.t1 | MTR_8g037175 | 94.737 | 532 | 26 | 2 | 1 | 531 | 1 | 531 | 0.0 | 991 |
| MS.gene016686.t1 | MTR_3g029510 | 74.019 | 535 | 122 | 5 | 1 | 530 | 17 | 539 | 0.0 | 791 |
| MS.gene016686.t1 | MTR_8g036660 | 72.296 | 527 | 137 | 5 | 4 | 527 | 87 | 607 | 0.0 | 763 |
| MS.gene016686.t1 | MTR_8g037170 | 70.336 | 536 | 143 | 6 | 5 | 527 | 20 | 552 | 0.0 | 762 |
| MS.gene016686.t1 | MTR_8g037170 | 72.433 | 526 | 136 | 5 | 5 | 527 | 20 | 539 | 0.0 | 762 |
| MS.gene016686.t1 | MTR_2g097900 | 57.553 | 523 | 185 | 5 | 6 | 527 | 21 | 507 | 0.0 | 567 |
| MS.gene016686.t1 | MTR_2g097900 | 57.029 | 377 | 125 | 5 | 6 | 381 | 21 | 361 | 3.75e-130 | 385 |
| MS.gene016686.t1 | MTR_5g099020 | 41.398 | 372 | 214 | 2 | 159 | 528 | 182 | 551 | 6.79e-95 | 300 |
| MS.gene016686.t1 | MTR_5g099020 | 40.217 | 92 | 51 | 1 | 19 | 110 | 112 | 199 | 2.46e-14 | 76.3 |
| MS.gene016686.t1 | MTR_5g099020 | 41.398 | 372 | 214 | 2 | 159 | 528 | 182 | 551 | 1.72e-94 | 299 |
| MS.gene016686.t1 | MTR_5g099020 | 40.217 | 92 | 51 | 1 | 19 | 110 | 112 | 199 | 2.46e-14 | 76.3 |
| MS.gene016686.t1 | MTR_7g070210 | 44.487 | 263 | 146 | 0 | 171 | 433 | 204 | 466 | 7.62e-63 | 213 |
| MS.gene016686.t1 | MTR_7g070210 | 61.538 | 78 | 30 | 0 | 14 | 91 | 110 | 187 | 2.87e-16 | 82.0 |
| MS.gene016686.t1 | MTR_8g037130 | 100.000 | 52 | 0 | 0 | 1 | 52 | 1 | 52 | 3.32e-26 | 101 |
| MS.gene016686.t1 | MTR_4g071370 | 25.217 | 345 | 217 | 12 | 198 | 520 | 207 | 532 | 7.87e-11 | 65.1 |
| MS.gene016686.t1 | MTR_4g071390 | 25.628 | 398 | 265 | 16 | 136 | 515 | 129 | 513 | 9.93e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016686.t1 | AT3G08040 | 61.407 | 526 | 177 | 6 | 5 | 528 | 19 | 520 | 0.0 | 597 |
| MS.gene016686.t1 | AT1G51340 | 57.224 | 526 | 198 | 6 | 3 | 527 | 16 | 515 | 0.0 | 546 |
| MS.gene016686.t1 | AT1G51340 | 57.224 | 526 | 198 | 6 | 3 | 527 | 16 | 515 | 0.0 | 546 |
| MS.gene016686.t1 | AT1G51340 | 57.224 | 526 | 198 | 6 | 3 | 527 | 16 | 515 | 0.0 | 546 |
| MS.gene016686.t1 | AT1G51340 | 57.364 | 516 | 193 | 6 | 13 | 527 | 20 | 509 | 0.0 | 537 |
| MS.gene016686.t1 | AT3G08040 | 60.688 | 407 | 147 | 2 | 128 | 527 | 15 | 415 | 3.47e-173 | 495 |
| MS.gene016686.t1 | AT1G51340 | 55.860 | 401 | 150 | 6 | 3 | 402 | 16 | 390 | 2.78e-129 | 382 |
| MS.gene016686.t1 | AT4G38380 | 39.062 | 512 | 249 | 6 | 19 | 528 | 33 | 483 | 1.35e-109 | 335 |
| MS.gene016686.t1 | AT4G38380 | 39.062 | 512 | 249 | 6 | 19 | 528 | 105 | 555 | 3.86e-109 | 337 |
| MS.gene016686.t1 | AT4G38380 | 39.062 | 512 | 249 | 6 | 19 | 528 | 105 | 555 | 3.86e-109 | 337 |
| MS.gene016686.t1 | AT4G38380 | 39.208 | 505 | 244 | 6 | 26 | 528 | 2 | 445 | 2.34e-108 | 331 |
| MS.gene016686.t1 | AT4G38380 | 38.477 | 512 | 242 | 5 | 19 | 528 | 105 | 545 | 1.01e-106 | 330 |
| MS.gene016686.t1 | AT2G38330 | 43.923 | 362 | 199 | 2 | 166 | 525 | 154 | 513 | 8.95e-96 | 301 |
| MS.gene016686.t1 | AT2G38330 | 54.118 | 85 | 35 | 1 | 22 | 102 | 79 | 163 | 4.92e-14 | 75.1 |
| MS.gene016686.t1 | AT2G21340 | 26.316 | 342 | 208 | 13 | 198 | 515 | 216 | 537 | 6.39e-13 | 71.6 |
| MS.gene016686.t1 | AT2G21340 | 26.048 | 334 | 203 | 13 | 206 | 515 | 221 | 534 | 1.71e-11 | 67.0 |
Find 85 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACTGCAATACTTATATT+TGG | 0.156175 | 8.1:-66356101 | MS.gene016686:CDS |
| GCACTTTCTCGGGAGATATT+AGG | 0.187730 | 8.1:-66364252 | MS.gene016686:CDS |
| GTATTTGATGGTGTGAATTA+TGG | 0.241654 | 8.1:-66353332 | MS.gene016686:CDS |
| ATGCATGCATCTATTTGTTT+TGG | 0.249530 | 8.1:+66356232 | None:intergenic |
| TAAGTATTGCAGTTTGAATT+AGG | 0.261020 | 8.1:+66356108 | None:intergenic |
| TGGTTTGGCTGTTGCTGTTC+AGG | 0.268976 | 8.1:-66354307 | MS.gene016686:intron |
| GTTGTTCATCTAATCAAAAT+TGG | 0.270374 | 8.1:-66353508 | MS.gene016686:CDS |
| TCCTGCTGTTCTTCTCTCAT+TGG | 0.275446 | 8.1:-66355521 | MS.gene016686:CDS |
| TTCAACCTTGCTTACAAATC+TGG | 0.277017 | 8.1:-66354353 | MS.gene016686:CDS |
| ATCACTTCTTGCTGATGGTT+TGG | 0.290135 | 8.1:-66354322 | MS.gene016686:CDS |
| TTCGCATGTTTGCTGGTATA+TGG | 0.293568 | 8.1:-66353075 | MS.gene016686:CDS |
| CACTTTCTCGGGAGATATTA+GGG | 0.298009 | 8.1:-66364251 | MS.gene016686:CDS |
| TCAAATAAAAGATGAGTATT+GGG | 0.319263 | 8.1:+66355188 | None:intergenic |
| TTACTTCAAGACATAGAAAA+TGG | 0.322449 | 8.1:-66357585 | MS.gene016686:CDS |
| GCATTAGCCTTTGTATTTGA+TGG | 0.325766 | 8.1:-66353344 | MS.gene016686:CDS |
| TCTTGAAGTAACTGATCCTT+AGG | 0.336949 | 8.1:+66357597 | None:intergenic |
| AATACTAACAAGAGGAAATA+TGG | 0.342556 | 8.1:+66357719 | None:intergenic |
| GGCCAAGCATTGTGCCAAAA+AGG | 0.350399 | 8.1:+66356129 | None:intergenic |
| TACTTAAGATTGAGAGCGTT+TGG | 0.351891 | 8.1:-66355547 | MS.gene016686:CDS |
| TTCAAATAAAAGATGAGTAT+TGG | 0.354147 | 8.1:+66355187 | None:intergenic |
| CTTATTGATACAGCTTTCAT+AGG | 0.356167 | 8.1:-66364180 | MS.gene016686:CDS |
| ATGATTCTTCGCATGTTTGC+TGG | 0.361880 | 8.1:-66353082 | MS.gene016686:CDS |
| CTCTCTGTACTTGTTGGAGT+TGG | 0.364912 | 8.1:-66353571 | MS.gene016686:CDS |
| TTAAGGATTTGCAGATATTC+CGG | 0.366921 | 8.1:-66354716 | MS.gene016686:CDS |
| TGCAGGATGGGAACAGCAAC+TGG | 0.371504 | 8.1:-66352872 | MS.gene016686:intron |
| CTTTGTTCAACCAAGCTTCA+AGG | 0.371536 | 8.1:-66357746 | MS.gene016686:CDS |
| TTATCCTTATAGGTACTTGA+TGG | 0.375374 | 8.1:-66354793 | MS.gene016686:intron |
| TATGACCTTTGCAGCATCAT+TGG | 0.384522 | 8.1:-66354409 | MS.gene016686:CDS |
| TTCAACATTTGTGTCATTGT+TGG | 0.389395 | 8.1:+66357521 | None:intergenic |
| ACAAAGGCTAATGCATTGAT+TGG | 0.393769 | 8.1:+66353353 | None:intergenic |
| AACTCACGGATGACATATAA+AGG | 0.399714 | 8.1:+66355457 | None:intergenic |
| GCAATGCAAGGAATCTTTAG+AGG | 0.402148 | 8.1:-66355499 | MS.gene016686:CDS |
| AATCTTAAGTATTTCACTGC+TGG | 0.426605 | 8.1:+66355559 | None:intergenic |
| TATTTGAAATTGGGACTCAA+TGG | 0.433342 | 8.1:-66355172 | MS.gene016686:CDS |
| CATGCGAAGAATCATATAGA+TGG | 0.440045 | 8.1:+66353090 | None:intergenic |
| TCAAGCTAGATGCACTTTCT+CGG | 0.440700 | 8.1:-66364263 | MS.gene016686:CDS |
| GGAATATCTGCAAATCCTTA+AGG | 0.446422 | 8.1:+66354718 | None:intergenic |
| ATATGCATTGAATGTCATAT+TGG | 0.447465 | 8.1:-66355212 | MS.gene016686:CDS |
| AGCATGTTCCTTTACTGAAA+AGG | 0.447695 | 8.1:-66353659 | MS.gene016686:CDS |
| ACATCATCACTTCTTGCTGA+TGG | 0.449462 | 8.1:-66354327 | MS.gene016686:CDS |
| TCTCCTACCTCCAAGCCTTA+AGG | 0.450343 | 8.1:-66354733 | MS.gene016686:CDS |
| ATCTGCAAATCCTTAAGGCT+TGG | 0.456124 | 8.1:+66354723 | None:intergenic |
| CAAGCTAGATGCACTTTCTC+GGG | 0.456268 | 8.1:-66364262 | MS.gene016686:CDS |
| GCATCATTGGCAGCAAGGTT+AGG | 0.456825 | 8.1:-66354396 | MS.gene016686:CDS |
| TACAGCTTTCATAGGCCACT+TGG | 0.460167 | 8.1:-66364172 | MS.gene016686:CDS |
| TAAACTTCTATTAGCTGCCA+TGG | 0.460920 | 8.1:-66356075 | MS.gene016686:CDS |
| GCAAACAATTCATCGGCATA+TGG | 0.461763 | 8.1:-66353123 | MS.gene016686:CDS |
| TTGCAGAATTCTTTCTCTAT+CGG | 0.465825 | 8.1:-66353147 | MS.gene016686:CDS |
| AAGGTTAGGTTCAGTTCCTA+TGG | 0.469099 | 8.1:-66354382 | MS.gene016686:CDS |
| GCCAATGAGAGAAGAACAGC+AGG | 0.483129 | 8.1:+66355520 | None:intergenic |
| ATGGGACTCTCTGTACTTGT+TGG | 0.485339 | 8.1:-66353577 | MS.gene016686:CDS |
| CTTGAAGTAACTGATCCTTA+GGG | 0.487610 | 8.1:+66357598 | None:intergenic |
| CCGGTGGAACTTGCTGCAGC+AGG | 0.492147 | 8.1:-66357780 | MS.gene016686:CDS |
| GACTACAGGTGGTTTATTGT+TGG | 0.498151 | 8.1:-66354457 | MS.gene016686:intron |
| AACAAGAGTAAAGACAAAGT+TGG | 0.504018 | 8.1:-66356191 | MS.gene016686:CDS |
| TGCAAATCCTTAAGGCTTGG+AGG | 0.504314 | 8.1:+66354726 | None:intergenic |
| ATATGGTAATCCTTGAAGCT+TGG | 0.508656 | 8.1:+66357736 | None:intergenic |
| TCTAATCAAAATTGGAATCC+CGG | 0.523690 | 8.1:-66353500 | MS.gene016686:intron |
| TTGAAGTAACTGATCCTTAG+GGG | 0.532447 | 8.1:+66357599 | None:intergenic |
| AATGACACAAATGTTGAAGA+TGG | 0.532730 | 8.1:-66357516 | MS.gene016686:intron |
| CTTCTCTCATTGGCAATGCA+AGG | 0.540460 | 8.1:-66355511 | MS.gene016686:CDS |
| TACAACTTCCTTTGTAGCTG+AGG | 0.542038 | 8.1:-66357697 | MS.gene016686:CDS |
| CCTGCTGCAGCAAGTTCCAC+CGG | 0.543591 | 8.1:+66357780 | None:intergenic |
| TGGGAACAGCAACTGGACCT+TGG | 0.549124 | 8.1:-66352865 | MS.gene016686:CDS |
| TTGCAGCATCATTGGCAGCA+AGG | 0.551205 | 8.1:-66354401 | MS.gene016686:CDS |
| TGCTGCCAATGATGCTGCAA+AGG | 0.552578 | 8.1:+66354404 | None:intergenic |
| AGTATCTTCCTCAGCTACAA+AGG | 0.559486 | 8.1:+66357689 | None:intergenic |
| TATATTCTATGGCAATGAGT+TGG | 0.562568 | 8.1:+66352829 | None:intergenic |
| CAATGCAAGGAATCTTTAGA+GGG | 0.568460 | 8.1:-66355498 | MS.gene016686:CDS |
| GTCAACCAGATTTGTAAGCA+AGG | 0.571199 | 8.1:+66354348 | None:intergenic |
| GTTCATGTGGTTAGGACCGG+TGG | 0.575639 | 8.1:-66357796 | MS.gene016686:intron |
| ACAGCTTTCATAGGCCACTT+GGG | 0.582867 | 8.1:-66364171 | MS.gene016686:intron |
| AGCTTGGTTGAACAAAGCAA+TGG | 0.583345 | 8.1:+66357752 | None:intergenic |
| TATCGGAGCAAACAATTCAT+CGG | 0.585821 | 8.1:-66353130 | MS.gene016686:CDS |
| CAAGGTTGAAATGCAGCCAT+AGG | 0.587704 | 8.1:+66354366 | None:intergenic |
| AAACTTCTATTAGCTGCCAT+GGG | 0.597932 | 8.1:-66356074 | MS.gene016686:CDS |
| AATCCTTAAGGCTTGGAGGT+AGG | 0.603443 | 8.1:+66354730 | None:intergenic |
| GCAGCAACAGCTAATGCTGA+GGG | 0.607784 | 8.1:+66364219 | None:intergenic |
| ATTCACACCATCAAATACAA+AGG | 0.611394 | 8.1:+66353337 | None:intergenic |
| TGAAGTAACTGATCCTTAGG+GGG | 0.619693 | 8.1:+66357600 | None:intergenic |
| AGCAGCAACAGCTAATGCTG+AGG | 0.625032 | 8.1:+66364218 | None:intergenic |
| GAAGTTGTAATACTAACAAG+AGG | 0.634951 | 8.1:+66357711 | None:intergenic |
| GCATGTTTGCTGGTATATGG+AGG | 0.640538 | 8.1:-66353072 | MS.gene016686:intron |
| GTATCAATAAGAGAAGCAAG+AGG | 0.654812 | 8.1:+66364192 | None:intergenic |
| TTGGCTCTGAGATAGCTCCA+AGG | 0.690784 | 8.1:+66352848 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 66352835 | 66365207 | 66352835 | ID=MS.gene016686 |
| chr8.1 | mRNA | 66352835 | 66365207 | 66352835 | ID=MS.gene016686.t1;Parent=MS.gene016686 |
| chr8.1 | exon | 66365176 | 66365207 | 66365176 | ID=MS.gene016686.t1.exon1;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66365176 | 66365207 | 66365176 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66364172 | 66364293 | 66364172 | ID=MS.gene016686.t1.exon2;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66364172 | 66364293 | 66364172 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66357517 | 66357804 | 66357517 | ID=MS.gene016686.t1.exon3;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66357517 | 66357804 | 66357517 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66356064 | 66356257 | 66356064 | ID=MS.gene016686.t1.exon4;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66356064 | 66356257 | 66356064 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66355464 | 66355599 | 66355464 | ID=MS.gene016686.t1.exon5;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66355464 | 66355599 | 66355464 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66355145 | 66355241 | 66355145 | ID=MS.gene016686.t1.exon6;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66355145 | 66355241 | 66355145 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66354703 | 66354803 | 66354703 | ID=MS.gene016686.t1.exon7;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66354703 | 66354803 | 66354703 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66354308 | 66354471 | 66354308 | ID=MS.gene016686.t1.exon8;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66354308 | 66354471 | 66354308 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66353501 | 66353689 | 66353501 | ID=MS.gene016686.t1.exon9;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66353501 | 66353689 | 66353501 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66353298 | 66353393 | 66353298 | ID=MS.gene016686.t1.exon10;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66353298 | 66353393 | 66353298 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66353073 | 66353194 | 66353073 | ID=MS.gene016686.t1.exon11;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66353073 | 66353194 | 66353073 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| chr8.1 | exon | 66352835 | 66352889 | 66352835 | ID=MS.gene016686.t1.exon12;Parent=MS.gene016686.t1 |
| chr8.1 | CDS | 66352835 | 66352889 | 66352835 | ID=cds.MS.gene016686.t1;Parent=MS.gene016686.t1 |
| Gene Sequence |
| Protein sequence |
