Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017154.t1 | XP_003617481.1 | 97.2 | 281 | 5 | 1 | 1 | 281 | 12 | 289 | 3.90E-147 | 530.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017154.t1 | P87027 | 47.1 | 187 | 99 | 0 | 95 | 281 | 9 | 195 | 6.4e-50 | 199.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017154.t1 | G7K3G2 | 97.2 | 281 | 5 | 1 | 1 | 281 | 12 | 289 | 2.8e-147 | 530.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene95545 | MS.gene017154 | PPI |
| MS.gene017154 | MS.gene87637 | PPI |
| MS.gene027416 | MS.gene017154 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017154.t1 | MTR_5g092060 | 98.221 | 281 | 2 | 2 | 1 | 281 | 12 | 289 | 0.0 | 567 |
| MS.gene017154.t1 | MTR_4g047870 | 63.158 | 266 | 84 | 4 | 20 | 281 | 21 | 276 | 3.62e-112 | 325 |
| MS.gene017154.t1 | MTR_5g013900 | 27.485 | 171 | 118 | 3 | 92 | 261 | 5 | 170 | 1.30e-16 | 77.0 |
| MS.gene017154.t1 | MTR_1g062760 | 27.072 | 181 | 121 | 5 | 95 | 271 | 13 | 186 | 5.58e-16 | 75.1 |
| MS.gene017154.t1 | MTR_4g064897 | 28.824 | 170 | 113 | 2 | 97 | 264 | 13 | 176 | 7.86e-16 | 74.7 |
| MS.gene017154.t1 | MTR_4g098950 | 26.316 | 171 | 120 | 3 | 92 | 261 | 5 | 170 | 8.79e-16 | 74.7 |
| MS.gene017154.t1 | MTR_3g064390 | 30.588 | 170 | 108 | 4 | 95 | 261 | 9 | 171 | 1.28e-15 | 73.9 |
| MS.gene017154.t1 | MTR_3g069510 | 29.412 | 170 | 112 | 2 | 97 | 264 | 13 | 176 | 3.36e-15 | 73.2 |
| MS.gene017154.t1 | MTR_2g005510 | 27.811 | 169 | 116 | 2 | 97 | 264 | 16 | 179 | 4.38e-15 | 72.8 |
| MS.gene017154.t1 | MTR_5g076590 | 30.588 | 170 | 108 | 4 | 95 | 261 | 9 | 171 | 1.15e-14 | 71.2 |
| MS.gene017154.t1 | MTR_2g075950 | 28.415 | 183 | 118 | 5 | 87 | 264 | 3 | 177 | 1.96e-14 | 71.2 |
| MS.gene017154.t1 | MTR_2g083860 | 27.717 | 184 | 118 | 6 | 87 | 264 | 3 | 177 | 3.91e-14 | 70.1 |
| MS.gene017154.t1 | MTR_4g079350 | 25.521 | 192 | 134 | 4 | 87 | 276 | 3 | 187 | 9.32e-14 | 69.3 |
| MS.gene017154.t1 | MTR_1g078200 | 24.862 | 181 | 129 | 3 | 85 | 264 | 4 | 178 | 1.35e-13 | 68.9 |
| MS.gene017154.t1 | MTR_3g086980 | 26.744 | 172 | 114 | 5 | 97 | 264 | 17 | 180 | 1.68e-13 | 68.6 |
| MS.gene017154.t1 | MTR_3g087160 | 25.146 | 171 | 122 | 3 | 92 | 261 | 5 | 170 | 1.69e-13 | 68.2 |
| MS.gene017154.t1 | MTR_0262s0050 | 27.072 | 181 | 119 | 6 | 88 | 264 | 8 | 179 | 2.11e-13 | 68.2 |
| MS.gene017154.t1 | MTR_8g090215 | 24.561 | 171 | 123 | 3 | 92 | 261 | 5 | 170 | 2.59e-13 | 67.8 |
| MS.gene017154.t1 | MTR_1g068730 | 26.190 | 168 | 114 | 4 | 90 | 254 | 10 | 170 | 3.26e-13 | 67.8 |
| MS.gene017154.t1 | MTR_7g103070 | 26.923 | 182 | 121 | 5 | 95 | 271 | 15 | 189 | 4.29e-13 | 67.4 |
| MS.gene017154.t1 | MTR_8g103550 | 26.667 | 165 | 115 | 2 | 98 | 261 | 11 | 170 | 4.89e-13 | 66.6 |
| MS.gene017154.t1 | MTR_7g051940 | 24.118 | 170 | 119 | 4 | 97 | 263 | 14 | 176 | 4.95e-13 | 67.0 |
| MS.gene017154.t1 | MTR_1g068740 | 26.190 | 168 | 114 | 4 | 90 | 254 | 10 | 170 | 5.28e-13 | 67.0 |
| MS.gene017154.t1 | MTR_1g052475 | 28.070 | 171 | 113 | 4 | 97 | 264 | 16 | 179 | 5.36e-13 | 67.0 |
| MS.gene017154.t1 | MTR_7g100020 | 25.466 | 161 | 111 | 3 | 97 | 254 | 16 | 170 | 6.69e-13 | 66.6 |
| MS.gene017154.t1 | MTR_8g089985 | 26.744 | 172 | 114 | 5 | 97 | 264 | 18 | 181 | 8.66e-13 | 66.6 |
| MS.gene017154.t1 | MTR_7g405790 | 23.214 | 168 | 123 | 2 | 97 | 263 | 16 | 178 | 1.29e-12 | 65.9 |
| MS.gene017154.t1 | MTR_2g096550 | 23.125 | 160 | 117 | 2 | 97 | 254 | 16 | 171 | 1.30e-12 | 65.9 |
| MS.gene017154.t1 | MTR_2g005500 | 23.810 | 168 | 122 | 2 | 97 | 263 | 10 | 172 | 1.76e-12 | 65.5 |
| MS.gene017154.t1 | MTR_1g115325 | 24.845 | 161 | 112 | 3 | 97 | 254 | 16 | 170 | 2.06e-12 | 65.5 |
| MS.gene017154.t1 | MTR_4g088090 | 23.958 | 192 | 137 | 4 | 87 | 276 | 3 | 187 | 2.59e-12 | 65.1 |
| MS.gene017154.t1 | MTR_1g090717 | 25.556 | 180 | 125 | 4 | 96 | 271 | 15 | 189 | 4.29e-12 | 64.3 |
| MS.gene017154.t1 | MTR_2g014990 | 24.479 | 192 | 136 | 4 | 87 | 276 | 3 | 187 | 5.13e-12 | 64.3 |
| MS.gene017154.t1 | MTR_3g064390 | 30.508 | 118 | 81 | 1 | 95 | 211 | 9 | 126 | 6.61e-12 | 62.8 |
| MS.gene017154.t1 | MTR_2g090865 | 24.852 | 169 | 121 | 2 | 97 | 264 | 12 | 175 | 1.25e-11 | 63.2 |
| MS.gene017154.t1 | MTR_3g107717 | 26.012 | 173 | 121 | 1 | 93 | 265 | 10 | 175 | 1.60e-11 | 62.8 |
| MS.gene017154.t1 | MTR_3g107713 | 26.012 | 173 | 121 | 1 | 93 | 265 | 10 | 175 | 1.78e-11 | 62.8 |
| MS.gene017154.t1 | MTR_3g107707 | 26.012 | 173 | 121 | 1 | 93 | 265 | 10 | 175 | 1.84e-11 | 62.8 |
| MS.gene017154.t1 | MTR_7g117910 | 29.771 | 131 | 87 | 2 | 86 | 211 | 6 | 136 | 2.20e-11 | 62.4 |
| MS.gene017154.t1 | MTR_8g027220 | 26.316 | 190 | 125 | 5 | 80 | 261 | 5 | 187 | 5.39e-11 | 61.6 |
| MS.gene017154.t1 | MTR_4g063160 | 25.153 | 163 | 111 | 4 | 97 | 254 | 16 | 172 | 6.08e-11 | 61.2 |
| MS.gene017154.t1 | MTR_8g036220 | 25.294 | 170 | 115 | 5 | 97 | 262 | 17 | 178 | 9.37e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017154.t1 | AT5G54840 | 63.860 | 285 | 89 | 5 | 1 | 281 | 12 | 286 | 1.22e-118 | 342 |
| MS.gene017154.t1 | AT3G21700 | 54.054 | 296 | 117 | 6 | 1 | 281 | 1 | 292 | 3.87e-105 | 308 |
| MS.gene017154.t1 | AT3G21700 | 53.716 | 296 | 117 | 7 | 1 | 281 | 1 | 291 | 5.37e-103 | 302 |
| MS.gene017154.t1 | AT3G21700 | 48.347 | 242 | 105 | 7 | 1 | 227 | 1 | 237 | 4.24e-67 | 209 |
| MS.gene017154.t1 | AT5G54840 | 55.172 | 203 | 77 | 5 | 1 | 199 | 12 | 204 | 1.09e-60 | 192 |
| MS.gene017154.t1 | AT5G54840 | 55.172 | 203 | 77 | 5 | 1 | 199 | 12 | 204 | 3.17e-60 | 191 |
| MS.gene017154.t1 | AT2G44610 | 26.316 | 171 | 120 | 2 | 92 | 261 | 5 | 170 | 3.92e-17 | 78.6 |
| MS.gene017154.t1 | AT5G59150 | 27.841 | 176 | 122 | 2 | 89 | 264 | 6 | 176 | 2.04e-16 | 76.6 |
| MS.gene017154.t1 | AT1G09630 | 27.604 | 192 | 124 | 3 | 97 | 278 | 13 | 199 | 4.39e-16 | 75.9 |
| MS.gene017154.t1 | AT1G07410 | 28.235 | 170 | 114 | 3 | 97 | 264 | 13 | 176 | 9.41e-16 | 74.7 |
| MS.gene017154.t1 | AT3G46830 | 28.824 | 170 | 113 | 2 | 97 | 264 | 13 | 176 | 1.04e-15 | 74.7 |
| MS.gene017154.t1 | AT3G46830 | 28.824 | 170 | 113 | 2 | 97 | 264 | 13 | 176 | 1.04e-15 | 74.7 |
| MS.gene017154.t1 | AT1G05810 | 26.471 | 170 | 115 | 4 | 97 | 263 | 13 | 175 | 3.60e-15 | 73.2 |
| MS.gene017154.t1 | AT5G45130 | 29.412 | 170 | 110 | 4 | 95 | 261 | 9 | 171 | 8.86e-15 | 71.6 |
| MS.gene017154.t1 | AT1G05810 | 26.471 | 170 | 115 | 4 | 97 | 263 | 59 | 221 | 8.96e-15 | 72.8 |
| MS.gene017154.t1 | AT1G16920 | 26.289 | 194 | 130 | 5 | 87 | 276 | 3 | 187 | 1.35e-14 | 71.6 |
| MS.gene017154.t1 | AT4G19640 | 29.412 | 170 | 110 | 4 | 95 | 261 | 9 | 171 | 1.46e-14 | 71.2 |
| MS.gene017154.t1 | AT5G03530 | 26.111 | 180 | 124 | 4 | 95 | 271 | 12 | 185 | 1.70e-14 | 71.2 |
| MS.gene017154.t1 | AT5G03530 | 26.111 | 180 | 124 | 4 | 95 | 271 | 12 | 185 | 1.70e-14 | 71.2 |
| MS.gene017154.t1 | AT4G18800 | 28.415 | 183 | 118 | 5 | 87 | 264 | 3 | 177 | 2.29e-14 | 70.9 |
| MS.gene017154.t1 | AT5G65270 | 27.128 | 188 | 121 | 6 | 84 | 264 | 3 | 181 | 2.34e-14 | 71.2 |
| MS.gene017154.t1 | AT3G07410 | 27.647 | 170 | 113 | 4 | 97 | 263 | 13 | 175 | 3.27e-14 | 70.5 |
| MS.gene017154.t1 | AT2G43130 | 26.471 | 170 | 115 | 4 | 97 | 263 | 13 | 175 | 3.62e-14 | 70.5 |
| MS.gene017154.t1 | AT2G31680 | 26.036 | 169 | 115 | 4 | 97 | 262 | 13 | 174 | 3.77e-14 | 70.5 |
| MS.gene017154.t1 | AT4G39890 | 25.000 | 172 | 122 | 4 | 92 | 261 | 5 | 171 | 4.00e-14 | 70.1 |
| MS.gene017154.t1 | AT5G55080 | 28.090 | 178 | 111 | 4 | 89 | 261 | 6 | 171 | 4.54e-14 | 70.1 |
| MS.gene017154.t1 | AT4G39990 | 27.647 | 170 | 113 | 4 | 97 | 263 | 18 | 180 | 5.54e-14 | 70.1 |
| MS.gene017154.t1 | AT5G45750 | 28.415 | 183 | 118 | 5 | 87 | 264 | 3 | 177 | 8.53e-14 | 69.3 |
| MS.gene017154.t1 | AT3G15060 | 25.967 | 181 | 127 | 3 | 87 | 265 | 3 | 178 | 1.52e-13 | 68.6 |
| MS.gene017154.t1 | AT1G06400 | 28.022 | 182 | 120 | 5 | 87 | 264 | 3 | 177 | 1.72e-13 | 68.6 |
| MS.gene017154.t1 | AT5G10260 | 23.977 | 171 | 124 | 2 | 92 | 261 | 5 | 170 | 1.93e-13 | 68.2 |
| MS.gene017154.t1 | AT3G09900 | 26.190 | 168 | 114 | 4 | 90 | 254 | 10 | 170 | 2.04e-13 | 68.2 |
| MS.gene017154.t1 | AT5G47520 | 24.852 | 169 | 121 | 2 | 97 | 264 | 15 | 178 | 2.16e-13 | 68.2 |
| MS.gene017154.t1 | AT3G53610 | 26.347 | 167 | 113 | 4 | 97 | 260 | 16 | 175 | 2.34e-13 | 68.2 |
| MS.gene017154.t1 | AT3G53610 | 26.347 | 167 | 113 | 4 | 97 | 260 | 16 | 175 | 2.34e-13 | 68.2 |
| MS.gene017154.t1 | AT3G53610 | 26.347 | 167 | 113 | 4 | 97 | 260 | 16 | 175 | 2.34e-13 | 68.2 |
| MS.gene017154.t1 | AT2G22290 | 25.434 | 173 | 119 | 4 | 92 | 261 | 5 | 170 | 3.80e-13 | 67.4 |
| MS.gene017154.t1 | AT5G03520 | 26.190 | 168 | 114 | 4 | 90 | 254 | 10 | 170 | 3.99e-13 | 67.4 |
| MS.gene017154.t1 | AT5G59840 | 26.347 | 167 | 113 | 4 | 97 | 260 | 16 | 175 | 7.36e-13 | 66.6 |
| MS.gene017154.t1 | AT5G45130 | 29.268 | 164 | 106 | 4 | 101 | 261 | 23 | 179 | 7.78e-13 | 66.6 |
| MS.gene017154.t1 | AT5G60860 | 25.000 | 180 | 128 | 3 | 87 | 264 | 3 | 177 | 9.56e-13 | 66.2 |
| MS.gene017154.t1 | AT3G12160 | 26.744 | 172 | 114 | 5 | 97 | 264 | 16 | 179 | 1.28e-12 | 66.2 |
| MS.gene017154.t1 | AT3G46060 | 25.466 | 161 | 111 | 3 | 97 | 254 | 16 | 170 | 1.30e-12 | 65.9 |
| MS.gene017154.t1 | AT3G46060 | 25.466 | 161 | 111 | 3 | 97 | 254 | 16 | 170 | 1.30e-12 | 65.9 |
| MS.gene017154.t1 | AT3G46060 | 25.466 | 161 | 111 | 3 | 97 | 254 | 16 | 170 | 1.30e-12 | 65.9 |
| MS.gene017154.t1 | AT4G18430 | 26.087 | 184 | 123 | 6 | 86 | 264 | 2 | 177 | 1.59e-12 | 65.9 |
| MS.gene017154.t1 | AT4G19640 | 29.661 | 118 | 82 | 1 | 95 | 211 | 9 | 126 | 1.65e-12 | 64.3 |
| MS.gene017154.t1 | AT4G19640 | 29.661 | 118 | 82 | 1 | 95 | 211 | 9 | 126 | 2.25e-12 | 65.1 |
| MS.gene017154.t1 | AT1G01200 | 25.882 | 170 | 117 | 4 | 97 | 263 | 29 | 192 | 7.54e-12 | 64.3 |
| MS.gene017154.t1 | AT3G11730 | 25.904 | 166 | 117 | 3 | 97 | 261 | 9 | 169 | 1.24e-11 | 63.2 |
| MS.gene017154.t1 | AT5G47960 | 26.163 | 172 | 115 | 5 | 97 | 264 | 16 | 179 | 1.39e-11 | 63.2 |
| MS.gene017154.t1 | AT3G54840 | 25.137 | 183 | 126 | 4 | 83 | 261 | 20 | 195 | 2.33e-11 | 62.4 |
| MS.gene017154.t1 | AT5G20020 | 26.036 | 169 | 118 | 1 | 93 | 261 | 10 | 171 | 3.35e-11 | 62.0 |
| MS.gene017154.t1 | AT5G55190 | 26.036 | 169 | 118 | 1 | 93 | 261 | 10 | 171 | 4.31e-11 | 61.6 |
| MS.gene017154.t1 | AT5G20010 | 26.036 | 169 | 118 | 1 | 93 | 261 | 10 | 171 | 4.35e-11 | 61.6 |
Find 53 sgRNAs with CRISPR-Local
Find 143 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATTTAGAAGTCAATTA+TGG | 0.162376 | 5.3:+74015062 | None:intergenic |
| GTTCAGGTTGATGTTAAATA+TGG | 0.230732 | 5.3:-74017464 | MS.gene017154:CDS |
| AATGGAAGGATTGAATTTAA+TGG | 0.251785 | 5.3:-74016817 | MS.gene017154:CDS |
| ACAATTGTCCATTGTAAATC+TGG | 0.259047 | 5.3:+74015466 | None:intergenic |
| GAACAACAGCATACCAATAA+TGG | 0.260980 | 5.3:-74017251 | MS.gene017154:CDS |
| ATCTGACTCATACCCATTAT+TGG | 0.276525 | 5.3:+74017238 | None:intergenic |
| GTTCATTTGGAATAAACTTA+TGG | 0.296115 | 5.3:-74017402 | MS.gene017154:CDS |
| ACAATTTAGAAGTCAATTAT+GGG | 0.359970 | 5.3:+74015063 | None:intergenic |
| ACTGCAATTCCCATTCTAAT+AGG | 0.364632 | 5.3:-74015520 | MS.gene017154:CDS |
| TTGTTGGGTGATTGCCATAT+TGG | 0.368640 | 5.3:-74017188 | MS.gene017154:CDS |
| CAAAGCTCTTTAACTTGCCT+TGG | 0.379264 | 5.3:-74015116 | MS.gene017154:CDS |
| AAACTTATGGTTTGTTCTGT+TGG | 0.381679 | 5.3:-74017389 | MS.gene017154:CDS |
| ATAGAAAGAAATTTGAAAGT+TGG | 0.387772 | 5.3:-74015090 | MS.gene017154:CDS |
| AAACTTGGTTCCTATTAGAA+TGG | 0.395511 | 5.3:+74015510 | None:intergenic |
| AACCTTAAGATTAGTTTGTT+GGG | 0.398052 | 5.3:-74017203 | MS.gene017154:CDS |
| TGTTGGGTGATTGCCATATT+GGG | 0.408372 | 5.3:-74017187 | MS.gene017154:CDS |
| AGCCATGGGAATTTGATCTA+AGG | 0.420504 | 5.3:+74016554 | None:intergenic |
| GACAAAACTTTGTATGTTCA+AGG | 0.421423 | 5.3:-74016795 | MS.gene017154:CDS |
| ACTCTGCAGGAAAATTGTTC+AGG | 0.422078 | 5.3:-74017480 | MS.gene017154:CDS |
| GTGTTGCACTTGAAAAGAAA+AGG | 0.422846 | 5.3:+74015178 | None:intergenic |
| CACCCAACAAACTAATCTTA+AGG | 0.431894 | 5.3:+74017201 | None:intergenic |
| TTAAATATGGTATGCTTCAA+AGG | 0.431941 | 5.3:-74017451 | MS.gene017154:CDS |
| CAATTGTCCATTGTAAATCT+GGG | 0.433079 | 5.3:+74015467 | None:intergenic |
| AACAACAGCATACCAATAAT+GGG | 0.438949 | 5.3:-74017250 | MS.gene017154:CDS |
| GTCGCTGCCGTCTCCGGCTA+CGG | 0.440861 | 5.3:-74017294 | MS.gene017154:CDS |
| TCTCCGGCTACGGTTGATGA+TGG | 0.441368 | 5.3:-74017284 | MS.gene017154:CDS |
| AACTGCATCTTTACAAGCCA+TGG | 0.445088 | 5.3:+74016539 | None:intergenic |
| GGCTTATGCAAGGGCAATGA+AGG | 0.452873 | 5.3:-74015205 | MS.gene017154:CDS |
| GAACCTTAAGATTAGTTTGT+TGG | 0.454454 | 5.3:-74017204 | MS.gene017154:CDS |
| AGAAAAGAGGAGTTTGCAAA+TGG | 0.455607 | 5.3:-74016835 | MS.gene017154:CDS |
| AACTTGGTTCCTATTAGAAT+GGG | 0.455621 | 5.3:+74015511 | None:intergenic |
| GCCGTTGTCGCTGCCGTCTC+CGG | 0.463439 | 5.3:-74017300 | MS.gene017154:CDS |
| TGAAGCAAGAAAGTGGAATC+AGG | 0.481024 | 5.3:-74015916 | MS.gene017154:intron |
| AAGAGGAGTTTGCAAATGGA+AGG | 0.488557 | 5.3:-74016831 | MS.gene017154:CDS |
| GTATGAGTCAGATTCTGATT+TGG | 0.522092 | 5.3:-74017228 | MS.gene017154:CDS |
| TAAATATGGTATGCTTCAAA+GGG | 0.523765 | 5.3:-74017450 | MS.gene017154:CDS |
| AAATTTCTTTCTATCGTCCA+AGG | 0.528916 | 5.3:+74015099 | None:intergenic |
| AAACCATCATCAACCGTAGC+CGG | 0.529864 | 5.3:+74017281 | None:intergenic |
| TCTAACAAAATCATCAAACT+TGG | 0.542555 | 5.3:+74015495 | None:intergenic |
| AGAAGATGACACAACTCTGC+AGG | 0.552513 | 5.3:-74017493 | None:intergenic |
| GCCGGAGACGGCAGCGACAA+CGG | 0.555503 | 5.3:+74017299 | None:intergenic |
| TACAGGCAAGGGCTTATGCA+AGG | 0.559748 | 5.3:-74015215 | MS.gene017154:intron |
| TGTTGCACTTGAAAAGAAAA+GGG | 0.560066 | 5.3:+74015179 | None:intergenic |
| TAGGAAATGAGGTAGAAAAG+AGG | 0.564038 | 5.3:-74016848 | MS.gene017154:CDS |
| GTCCTTAGATCAAATTCCCA+TGG | 0.564993 | 5.3:-74016556 | MS.gene017154:CDS |
| GGTACAGTGAAGCAAGAAAG+TGG | 0.579957 | 5.3:-74015923 | MS.gene017154:CDS |
| AATTGTCCATTGTAAATCTG+GGG | 0.582951 | 5.3:+74015468 | None:intergenic |
| TCATCAACCGTAGCCGGAGA+CGG | 0.585501 | 5.3:+74017287 | None:intergenic |
| GATCAAGTACGTAGGAAATG+AGG | 0.589406 | 5.3:-74016859 | MS.gene017154:CDS |
| ACAATGGACAATTGTAACAC+AGG | 0.603332 | 5.3:-74015458 | MS.gene017154:intron |
| ACAGGCAAGGGCTTATGCAA+GGG | 0.634009 | 5.3:-74015214 | MS.gene017154:intron |
| ACTGCATCTTTACAAGCCAT+GGG | 0.635433 | 5.3:+74016540 | None:intergenic |
| ATTGTCCATTGTAAATCTGG+GGG | 0.655553 | 5.3:+74015469 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAAATATAGTAATATTT+CGG | + | chr5.3:74016528-74016547 | None:intergenic | 10.0% |
| !! | AAAAAAAAGGACTAATATAT+GGG | - | chr5.3:74016436-74016455 | MS.gene017154:intron | 15.0% |
| !! | AAAACATAATACATCTAATT+AGG | + | chr5.3:74016978-74016997 | None:intergenic | 15.0% |
| !! | AAACATAATACATCTAATTA+GGG | + | chr5.3:74016977-74016996 | None:intergenic | 15.0% |
| !!! | AAAAGTAACACTTTATTTAA+AGG | + | chr5.3:74017265-74017284 | None:intergenic | 15.0% |
| !!! | ATCATTTAACTTAACATTTT+TGG | - | chr5.3:74016187-74016206 | MS.gene017154:intron | 15.0% |
| !!! | ATTAGATGTATTATGTTTTA+TGG | - | chr5.3:74016979-74016998 | MS.gene017154:intron | 15.0% |
| !!! | TAAGGAAACATTTTAAATAA+AGG | - | chr5.3:74016243-74016262 | MS.gene017154:intron | 15.0% |
| !!! | TAAGTTTTATAGGAATATAA+TGG | - | chr5.3:74016557-74016576 | MS.gene017154:CDS | 15.0% |
| !!! | TTACTTTTTACCATATTATT+TGG | - | chr5.3:74017277-74017296 | MS.gene017154:CDS | 15.0% |
| !!! | TTTTATGCTATAAGTTTTAT+AGG | - | chr5.3:74016547-74016566 | MS.gene017154:CDS | 15.0% |
| !!! | TTTTTTAAGTTAGTTATTGA+TGG | - | chr5.3:74015941-74015960 | MS.gene017154:intron | 15.0% |
| !! | AATAACAAAGCCAAATAATA+TGG | + | chr5.3:74017290-74017309 | None:intergenic | 20.0% |
| !! | ATAGAAAGAAATTTGAAAGT+TGG | - | chr5.3:74017466-74017485 | MS.gene017154:CDS | 20.0% |
| !! | ATTGATGGAAAATTATTATG+TGG | - | chr5.3:74015956-74015975 | MS.gene017154:intron | 20.0% |
| !! | CTATATGTATTCGAAAAAAA+AGG | - | chr5.3:74016423-74016442 | MS.gene017154:intron | 20.0% |
| !! | GAAAAAAAAGGACTAATATA+TGG | - | chr5.3:74016435-74016454 | MS.gene017154:intron | 20.0% |
| !! | GAGAAAATCATATAATTCAA+AGG | + | chr5.3:74015570-74015589 | None:intergenic | 20.0% |
| !! | TAAAAAATAAATGCAGAACA+TGG | + | chr5.3:74015868-74015887 | None:intergenic | 20.0% |
| !! | TAACCTCAAAATTAAGTAAA+TGG | + | chr5.3:74016133-74016152 | None:intergenic | 20.0% |
| !! | TAAGTAAATGGAAAAAAAGA+AGG | + | chr5.3:74016121-74016140 | None:intergenic | 20.0% |
| !! | TTCTTACTATAATAGTTCAA+AGG | + | chr5.3:74016777-74016796 | None:intergenic | 20.0% |
| !! | TTGATGGAAAATTATTATGT+GGG | - | chr5.3:74015957-74015976 | MS.gene017154:intron | 20.0% |
| !! | TTTACCTTAAATGTTGAAAT+GGG | - | chr5.3:74015424-74015443 | MS.gene017154:intron | 20.0% |
| !!! | CAAAATTTTCAAGTTTATCA+TGG | - | chr5.3:74017417-74017436 | MS.gene017154:CDS | 20.0% |
| !!! | GTAAGTTCTGAAATATTATT+AGG | - | chr5.3:74016087-74016106 | MS.gene017154:intron | 20.0% |
| !!! | GTGACACTTTTTAAAATTAT+AGG | + | chr5.3:74016163-74016182 | None:intergenic | 20.0% |
| !!! | TATAATTGATATCGATTTGT+TGG | - | chr5.3:74015594-74015613 | MS.gene017154:intron | 20.0% |
| !!! | TATAGTAAGAAAAAGTTTTG+AGG | - | chr5.3:74016786-74016805 | MS.gene017154:CDS | 20.0% |
| !!! | TATTTTTAAAACCTATGCTT+TGG | - | chr5.3:74016315-74016334 | MS.gene017154:intron | 20.0% |
| !!! | TCGTATTTCATTTTGTATAT+GGG | - | chr5.3:74015787-74015806 | MS.gene017154:intron | 20.0% |
| !!! | TTTCCATTTACTTAATTTTG+AGG | - | chr5.3:74016127-74016146 | MS.gene017154:intron | 20.0% |
| !!! | TTTTTTAGATTTCTCTCATT+AGG | - | chr5.3:74015881-74015900 | MS.gene017154:intron | 20.0% |
| ! | AATGGAAGGATTGAATTTAA+TGG | - | chr5.3:74015739-74015758 | MS.gene017154:intron | 25.0% |
| ! | CTTTACCTTAAATGTTGAAA+TGG | - | chr5.3:74015423-74015442 | MS.gene017154:intron | 25.0% |
| ! | GAACCTCAAAATTTAGTTAA+AGG | - | chr5.3:74016485-74016504 | MS.gene017154:intron | 25.0% |
| ! | GTATGTATTTCATAGGTTAT+AGG | - | chr5.3:74015639-74015658 | MS.gene017154:intron | 25.0% |
| ! | TAAATATGGTATGCTTCAAA+GGG | - | chr5.3:74015106-74015125 | MS.gene017154:CDS | 25.0% |
| ! | TCACTGAAATTAAATTTCGT+TGG | - | chr5.3:74016684-74016703 | MS.gene017154:intron | 25.0% |
| ! | TCTAACAAAATCATCAAACT+TGG | + | chr5.3:74017064-74017083 | None:intergenic | 25.0% |
| ! | TGAGGTAGTTAAAATAGAAA+TGG | - | chr5.3:74016804-74016823 | MS.gene017154:CDS | 25.0% |
| ! | TGATGGAAAATTATTATGTG+GGG | - | chr5.3:74015958-74015977 | MS.gene017154:intron | 25.0% |
| ! | TTAAATATGGTATGCTTCAA+AGG | - | chr5.3:74015105-74015124 | MS.gene017154:CDS | 25.0% |
| ! | TTATTATGTGGGGTTTAATT+AGG | - | chr5.3:74015968-74015987 | MS.gene017154:intron | 25.0% |
| !! | AAAAAGTAGTTTTCCCAATA+TGG | + | chr5.3:74015385-74015404 | None:intergenic | 25.0% |
| !! | ATATGTTTGATTAGAAGTGT+CGG | + | chr5.3:74015507-74015526 | None:intergenic | 25.0% |
| !! | CTCGTATTTCATTTTGTATA+TGG | - | chr5.3:74015786-74015805 | MS.gene017154:intron | 25.0% |
| !! | GTAAGTGACATTAAATTTTC+AGG | - | chr5.3:74016889-74016908 | MS.gene017154:intron | 25.0% |
| !! | GTTCATTTGGAATAAACTTA+TGG | - | chr5.3:74015154-74015173 | MS.gene017154:CDS | 25.0% |
| !!! | AACCTTAAGATTAGTTTGTT+GGG | - | chr5.3:74015353-74015372 | MS.gene017154:intron | 25.0% |
| !!! | ATCTTAAGTGGATTCAATTT+TGG | - | chr5.3:74017152-74017171 | MS.gene017154:intron | 25.0% |
| !!! | CTAAATTTTGAGGTTCAAAT+TGG | + | chr5.3:74016481-74016500 | None:intergenic | 25.0% |
| !!! | TAACATTTTTGGTTCATTTG+TGG | - | chr5.3:74016198-74016217 | MS.gene017154:intron | 25.0% |
| !!! | TATTGGGAAAACTACTTTTT+TGG | - | chr5.3:74015385-74015404 | MS.gene017154:intron | 25.0% |
| !!! | TCTTAAGTGGATTCAATTTT+GGG | - | chr5.3:74017153-74017172 | MS.gene017154:intron | 25.0% |
| !!! | TTCATTGCTTTTCTTTTTGT+GGG | - | chr5.3:74017220-74017239 | MS.gene017154:CDS | 25.0% |
| AAACTTGGTTCCTATTAGAA+TGG | + | chr5.3:74017049-74017068 | None:intergenic | 30.0% | |
| AAATTTCTTTCTATCGTCCA+AGG | + | chr5.3:74017460-74017479 | None:intergenic | 30.0% | |
| AACAACAGCATACCAATAAT+GGG | - | chr5.3:74015306-74015325 | MS.gene017154:intron | 30.0% | |
| AACTTGGTTCCTATTAGAAT+GGG | + | chr5.3:74017048-74017067 | None:intergenic | 30.0% | |
| AATTGTCCATTGTAAATCTG+GGG | + | chr5.3:74017091-74017110 | None:intergenic | 30.0% | |
| ACAATTGTCCATTGTAAATC+TGG | + | chr5.3:74017093-74017112 | None:intergenic | 30.0% | |
| ATCTGGTGTATGTATTTCAT+AGG | - | chr5.3:74015632-74015651 | MS.gene017154:intron | 30.0% | |
| ATGACCCATTTCAACATTTA+AGG | + | chr5.3:74015431-74015450 | None:intergenic | 30.0% | |
| CAATTGTCCATTGTAAATCT+GGG | + | chr5.3:74017092-74017111 | None:intergenic | 30.0% | |
| CGAATACATATAGACAATGT+CGG | + | chr5.3:74016416-74016435 | None:intergenic | 30.0% | |
| GACAAAACTTTGTATGTTCA+AGG | - | chr5.3:74015761-74015780 | MS.gene017154:intron | 30.0% | |
| TCTGCAAATTGAATGTAACA+TGG | + | chr5.3:74017017-74017036 | None:intergenic | 30.0% | |
| TGATCAACTTACATCTTAAG+TGG | - | chr5.3:74017140-74017159 | MS.gene017154:intron | 30.0% | |
| TGTTGCACTTGAAAAGAAAA+GGG | + | chr5.3:74017380-74017399 | None:intergenic | 30.0% | |
| ! | AGTTGGAACTACATTTTACT+TGG | + | chr5.3:74016724-74016743 | None:intergenic | 30.0% |
| ! | ATTTCGGCTTACTTATTTCT+TGG | + | chr5.3:74016512-74016531 | None:intergenic | 30.0% |
| ! | CAACTACTAGTTTATAGTGA+AGG | - | chr5.3:74016363-74016382 | MS.gene017154:intron | 30.0% |
| ! | CTTTTGTTGGTCAGTTTTTT+AGG | - | chr5.3:74015132-74015151 | MS.gene017154:CDS | 30.0% |
| ! | GGTCCTTTAACTAAATTTTG+AGG | + | chr5.3:74016491-74016510 | None:intergenic | 30.0% |
| !! | AAACTTATGGTTTGTTCTGT+TGG | - | chr5.3:74015167-74015186 | MS.gene017154:CDS | 30.0% |
| !! | AATGAAAAGCATTTGGAAGT+TGG | + | chr5.3:74016741-74016760 | None:intergenic | 30.0% |
| !! | CTTAAGTGGATTCAATTTTG+GGG | - | chr5.3:74017154-74017173 | MS.gene017154:intron | 30.0% |
| !! | GAACCTTAAGATTAGTTTGT+TGG | - | chr5.3:74015352-74015371 | MS.gene017154:intron | 30.0% |
| !! | GTGTGAAAATGAAAAGCATT+TGG | + | chr5.3:74016748-74016767 | None:intergenic | 30.0% |
| !! | GTTCAGGTTGATGTTAAATA+TGG | - | chr5.3:74015092-74015111 | MS.gene017154:CDS | 30.0% |
| !! | GTTCATTGCTTTTCTTTTTG+TGG | - | chr5.3:74017219-74017238 | MS.gene017154:CDS | 30.0% |
| !!! | AATTTTGAGGTTCAAATTGG+TGG | + | chr5.3:74016478-74016497 | None:intergenic | 30.0% |
| !!! | CATTTTAGTGTTGACTATGT+TGG | - | chr5.3:74015462-74015481 | MS.gene017154:CDS | 30.0% |
| !!! | GTCAGTTTTTTAGGTTCATT+TGG | - | chr5.3:74015141-74015160 | MS.gene017154:CDS | 30.0% |
| !!! | TTTTTTCAGATCAAGTACGT+AGG | - | chr5.3:74015689-74015708 | MS.gene017154:intron | 30.0% |
| ACAATGGACAATTGTAACAC+AGG | - | chr5.3:74017098-74017117 | MS.gene017154:intron | 35.0% | |
| ACTAATATATGGGACCAATC+TGG | - | chr5.3:74016446-74016465 | MS.gene017154:intron | 35.0% | |
| ACTGCAATTCCCATTCTAAT+AGG | - | chr5.3:74017036-74017055 | MS.gene017154:intron | 35.0% | |
| ACTTTAACCAACATGCATGT+AGG | + | chr5.3:74017180-74017199 | None:intergenic | 35.0% | |
| AGAAAAGAGGAGTTTGCAAA+TGG | - | chr5.3:74015721-74015740 | MS.gene017154:intron | 35.0% | |
| ATCTGACTCATACCCATTAT+TGG | + | chr5.3:74015321-74015340 | None:intergenic | 35.0% | |
| ATTGTCCATTGTAAATCTGG+GGG | + | chr5.3:74017090-74017109 | None:intergenic | 35.0% | |
| CACCCAACAAACTAATCTTA+AGG | + | chr5.3:74015358-74015377 | None:intergenic | 35.0% | |
| CTAATATATGGGACCAATCT+GGG | - | chr5.3:74016447-74016466 | MS.gene017154:intron | 35.0% | |
| GAACAACAGCATACCAATAA+TGG | - | chr5.3:74015305-74015324 | MS.gene017154:intron | 35.0% | |
| GACTTGTACCTTTCATATAC+AGG | - | chr5.3:74017324-74017343 | MS.gene017154:CDS | 35.0% | |
| GGTCTTTGAATGTTTGAATC+TGG | - | chr5.3:74015615-74015634 | MS.gene017154:intron | 35.0% | |
| GTATGAGTCAGATTCTGATT+TGG | - | chr5.3:74015328-74015347 | MS.gene017154:intron | 35.0% | |
| GTGTTGCACTTGAAAAGAAA+AGG | + | chr5.3:74017381-74017400 | None:intergenic | 35.0% | |
| TACCTTTCATATACAGGCAA+GGG | - | chr5.3:74017330-74017349 | MS.gene017154:CDS | 35.0% | |
| TAGGAAATGAGGTAGAAAAG+AGG | - | chr5.3:74015708-74015727 | MS.gene017154:intron | 35.0% | |
| TGAAACTGTAGTAGCCTTTA+TGG | - | chr5.3:74016856-74016875 | MS.gene017154:CDS | 35.0% | |
| TTACCACTTATCAGCCATAA+AGG | + | chr5.3:74016873-74016892 | None:intergenic | 35.0% | |
| ! | TGGAACTACATTTTACTTGG+TGG | + | chr5.3:74016721-74016740 | None:intergenic | 35.0% |
| ! | TTTTGTATATGGGATGTTGC+AGG | - | chr5.3:74015797-74015816 | MS.gene017154:intron | 35.0% |
| !!! | CTTCAAAGGGTATCTTTTGT+TGG | - | chr5.3:74015119-74015138 | MS.gene017154:CDS | 35.0% |
| AACATGGAGTTGACATGGTA+AGG | - | chr5.3:74016225-74016244 | MS.gene017154:intron | 40.0% | |
| AACCTTCTTCTCCAAAGCAT+AGG | + | chr5.3:74016329-74016348 | None:intergenic | 40.0% | |
| AACTGCATCTTTACAAGCCA+TGG | + | chr5.3:74016020-74016039 | None:intergenic | 40.0% | |
| AAGAGGAGTTTGCAAATGGA+AGG | - | chr5.3:74015725-74015744 | MS.gene017154:intron | 40.0% | |
| ACTCTGCAGGAAAATTGTTC+AGG | - | chr5.3:74015076-74015095 | MS.gene017154:CDS | 40.0% | |
| ACTGCATCTTTACAAGCCAT+GGG | + | chr5.3:74016019-74016038 | None:intergenic | 40.0% | |
| AGCCATGGGAATTTGATCTA+AGG | + | chr5.3:74016005-74016024 | None:intergenic | 40.0% | |
| GATCAAGTACGTAGGAAATG+AGG | - | chr5.3:74015697-74015716 | MS.gene017154:intron | 40.0% | |
| GTACCTTTCATATACAGGCA+AGG | - | chr5.3:74017329-74017348 | MS.gene017154:CDS | 40.0% | |
| GTCCTTAGATCAAATTCCCA+TGG | - | chr5.3:74016000-74016019 | MS.gene017154:intron | 40.0% | |
| TAGCCTTTATGGCTGATAAG+TGG | - | chr5.3:74016867-74016886 | MS.gene017154:intron | 40.0% | |
| TGAAGCAAGAAAGTGGAATC+AGG | - | chr5.3:74016640-74016659 | MS.gene017154:intron | 40.0% | |
| TGTTGGGTGATTGCCATATT+GGG | - | chr5.3:74015369-74015388 | MS.gene017154:intron | 40.0% | |
| TTGTTGGGTGATTGCCATAT+TGG | - | chr5.3:74015368-74015387 | MS.gene017154:intron | 40.0% | |
| !! | AACCTATGCTTTGGAGAAGA+AGG | - | chr5.3:74016324-74016343 | MS.gene017154:intron | 40.0% |
| !! | ATTCAAAGGCGTCTCAATGT+CGG | + | chr5.3:74015556-74015575 | None:intergenic | 40.0% |
| !! | CAAAGCTCTTTAACTTGCCT+TGG | - | chr5.3:74017440-74017459 | MS.gene017154:CDS | 40.0% |
| !! | CTGTGTTTTTGCAGTGTTGT+TGG | - | chr5.3:74016608-74016627 | MS.gene017154:intron | 40.0% |
| !! | GTTCATTTGTGGCTCTAACA+TGG | - | chr5.3:74016209-74016228 | MS.gene017154:intron | 40.0% |
| !! | GTTTTTGCAGTGTTGTTGGA+TGG | - | chr5.3:74016612-74016631 | MS.gene017154:intron | 40.0% |
| AAACCATCATCAACCGTAGC+CGG | + | chr5.3:74015278-74015297 | None:intergenic | 45.0% | |
| AGCCCTTGCCTGTATATGAA+AGG | + | chr5.3:74017335-74017354 | None:intergenic | 45.0% | |
| ATATATGGGACCAATCTGGG+TGG | - | chr5.3:74016450-74016469 | MS.gene017154:intron | 45.0% | |
| GACTTCCCCCAGATTTACAA+TGG | - | chr5.3:74017082-74017101 | MS.gene017154:intron | 45.0% | |
| GCTCTAACATGGAGTTGACA+TGG | - | chr5.3:74016220-74016239 | MS.gene017154:intron | 45.0% | |
| GGTACAGTGAAGCAAGAAAG+TGG | - | chr5.3:74016633-74016652 | MS.gene017154:intron | 45.0% | |
| ACAGGCAAGGGCTTATGCAA+GGG | - | chr5.3:74017342-74017361 | MS.gene017154:CDS | 50.0% | |
| GGCTTATGCAAGGGCAATGA+AGG | - | chr5.3:74017351-74017370 | MS.gene017154:CDS | 50.0% | |
| TACAGGCAAGGGCTTATGCA+AGG | - | chr5.3:74017341-74017360 | MS.gene017154:CDS | 50.0% | |
| ! | TTTGGGGCCTACATGCATGT+TGG | - | chr5.3:74017170-74017189 | MS.gene017154:intron | 50.0% |
| TCATCAACCGTAGCCGGAGA+CGG | + | chr5.3:74015272-74015291 | None:intergenic | 55.0% | |
| ! | ATTGGTGGCTCCACCCAGAT+TGG | + | chr5.3:74016463-74016482 | None:intergenic | 55.0% |
| !! | TCTCCGGCTACGGTTGATGA+TGG | - | chr5.3:74015272-74015291 | MS.gene017154:intron | 55.0% |
| GCCGGAGACGGCAGCGACAA+CGG | + | chr5.3:74015260-74015279 | None:intergenic | 70.0% | |
| GCCGTTGTCGCTGCCGTCTC+CGG | - | chr5.3:74015256-74015275 | MS.gene017154:intron | 70.0% | |
| GTCGCTGCCGTCTCCGGCTA+CGG | - | chr5.3:74015262-74015281 | MS.gene017154:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 74015068 | 74017510 | 74015068 | ID=MS.gene017154 |
| chr5.3 | mRNA | 74015068 | 74017510 | 74015068 | ID=MS.gene017154.t1;Parent=MS.gene017154 |
| chr5.3 | exon | 74017172 | 74017510 | 74017172 | ID=MS.gene017154.t1.exon1;Parent=MS.gene017154.t1 |
| chr5.3 | CDS | 74017172 | 74017510 | 74017172 | ID=cds.MS.gene017154.t1;Parent=MS.gene017154.t1 |
| chr5.3 | exon | 74016760 | 74016880 | 74016760 | ID=MS.gene017154.t1.exon2;Parent=MS.gene017154.t1 |
| chr5.3 | CDS | 74016760 | 74016880 | 74016760 | ID=cds.MS.gene017154.t1;Parent=MS.gene017154.t1 |
| chr5.3 | exon | 74016492 | 74016588 | 74016492 | ID=MS.gene017154.t1.exon3;Parent=MS.gene017154.t1 |
| chr5.3 | CDS | 74016492 | 74016588 | 74016492 | ID=cds.MS.gene017154.t1;Parent=MS.gene017154.t1 |
| chr5.3 | exon | 74015917 | 74015956 | 74015917 | ID=MS.gene017154.t1.exon4;Parent=MS.gene017154.t1 |
| chr5.3 | CDS | 74015917 | 74015956 | 74015917 | ID=cds.MS.gene017154.t1;Parent=MS.gene017154.t1 |
| chr5.3 | exon | 74015459 | 74015542 | 74015459 | ID=MS.gene017154.t1.exon5;Parent=MS.gene017154.t1 |
| chr5.3 | CDS | 74015459 | 74015542 | 74015459 | ID=cds.MS.gene017154.t1;Parent=MS.gene017154.t1 |
| chr5.3 | exon | 74015068 | 74015232 | 74015068 | ID=MS.gene017154.t1.exon6;Parent=MS.gene017154.t1 |
| chr5.3 | CDS | 74015068 | 74015232 | 74015068 | ID=cds.MS.gene017154.t1;Parent=MS.gene017154.t1 |
| Gene Sequence |
| Protein sequence |
