AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020466


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020466.t1 MTR_3g099680 86.916 107 14 0 1 107 17 123 3.37e-66 202
MS.gene020466.t1 MTR_3g099980 85.981 107 15 0 1 107 17 123 5.28e-65 199
MS.gene020466.t1 MTR_3g099570 65.169 178 48 7 1 174 17 184 3.49e-61 187
MS.gene020466.t1 MTR_3g099580 65.169 178 50 5 1 174 17 186 2.61e-57 178
MS.gene020466.t1 MTR_3g099540 60.345 116 44 2 1 116 18 131 6.24e-39 129
MS.gene020466.t1 MTR_8g007020 44.886 176 83 5 1 169 14 182 3.39e-33 116
MS.gene020466.t1 MTR_8g007035 56.566 99 41 2 12 109 26 123 1.83e-30 110
MS.gene020466.t1 MTR_4g114870 46.610 118 58 2 8 121 27 143 1.34e-27 106
MS.gene020466.t1 MTR_4g114870 47.525 101 48 2 10 106 191 290 2.53e-25 100
MS.gene020466.t1 MTR_5g006040 36.310 168 96 6 5 170 21 179 3.29e-27 101
MS.gene020466.t1 MTR_1g090190 34.356 163 102 4 8 169 21 179 3.82e-27 100
MS.gene020466.t1 MTR_7g086140 42.718 103 59 0 7 109 23 125 2.26e-23 90.9
MS.gene020466.t1 MTR_7g086190 42.718 103 59 0 7 109 23 125 2.26e-23 90.9
MS.gene020466.t1 MTR_7g086160 41.748 103 60 0 7 109 24 126 2.53e-22 88.2
MS.gene020466.t1 MTR_1g105130 42.268 97 56 0 10 106 25 121 4.36e-22 87.4
MS.gene020466.t1 MTR_1g105120 42.268 97 56 0 10 106 25 121 4.36e-22 87.4
MS.gene020466.t1 MTR_8g099220 42.718 103 55 2 8 107 27 128 8.27e-22 85.9
MS.gene020466.t1 MTR_6g080660 46.154 104 54 2 7 110 25 126 1.05e-21 87.8
MS.gene020466.t1 MTR_1g104800 40.000 100 57 2 9 106 24 122 2.11e-21 86.3
MS.gene020466.t1 MTR_2g088990 39.259 135 72 5 7 139 22 148 3.56e-21 86.7
MS.gene020466.t1 MTR_7g086220 40.000 100 60 0 10 109 25 124 6.36e-21 84.3
MS.gene020466.t1 MTR_1g112700 38.318 107 64 2 8 113 31 136 6.49e-21 85.1
MS.gene020466.t1 MTR_6g083240 37.226 137 77 4 12 142 29 162 1.13e-20 84.3
MS.gene020466.t1 MTR_0334s0010 41.121 107 58 3 5 108 23 127 3.53e-19 80.9
MS.gene020466.t1 MTR_4g067200 38.793 116 64 4 1 110 19 133 3.08e-18 77.8
MS.gene020466.t1 MTR_4g066110 38.793 116 64 4 1 110 19 133 3.08e-18 77.8
MS.gene020466.t1 MTR_2g025580 36.296 135 80 4 1 130 18 151 2.20e-17 75.5
MS.gene020466.t1 MTR_6g023760 40.404 99 58 1 8 106 24 121 2.87e-17 75.5
MS.gene020466.t1 MTR_3g089035 43.158 95 50 3 10 104 33 123 4.24e-17 73.6
MS.gene020466.t1 MTR_2g083250 43.269 104 52 3 11 108 27 129 8.23e-17 75.9
MS.gene020466.t1 MTR_4g114830 39.560 91 49 2 11 95 30 120 1.27e-16 73.2
MS.gene020466.t1 MTR_7g086090 40.206 97 57 1 11 107 27 122 3.94e-16 73.6
MS.gene020466.t1 MTR_1g090420 28.750 160 90 5 7 147 25 179 6.01e-16 72.8
MS.gene020466.t1 MTR_2g043730 40.625 96 53 3 10 105 32 123 8.34e-16 70.1
MS.gene020466.t1 MTR_7g086280 38.776 98 59 1 10 107 26 122 1.15e-15 71.2
MS.gene020466.t1 MTR_7g086230 38.776 98 59 1 10 107 26 122 1.21e-15 71.2
MS.gene020466.t1 MTR_6g013420 36.000 125 69 4 8 123 36 158 1.25e-15 71.2
MS.gene020466.t1 MTR_8g463180 33.051 118 73 3 5 117 19 135 1.34e-15 73.2
MS.gene020466.t1 MTR_4g081100 38.889 90 51 1 11 96 33 122 1.43e-15 71.6
MS.gene020466.t1 MTR_2g101300 36.000 125 70 4 12 131 29 148 2.07e-15 70.9
MS.gene020466.t1 MTR_7g086100 39.583 96 57 1 11 106 27 121 8.80e-15 69.7
MS.gene020466.t1 MTR_4g007250 36.538 104 64 2 4 107 23 124 8.93e-15 68.6
MS.gene020466.t1 MTR_6g013200 35.294 119 71 4 11 127 36 150 1.02e-14 68.6
MS.gene020466.t1 MTR_8g088830 40.909 88 48 3 12 98 30 114 1.23e-14 67.0
MS.gene020466.t1 MTR_2g043790 40.625 96 53 3 10 105 7 98 1.38e-14 66.2
MS.gene020466.t1 MTR_8g086360 29.268 164 111 4 12 173 26 186 2.00e-14 68.2
MS.gene020466.t1 MTR_8g089110 37.500 96 56 3 10 105 28 119 1.89e-13 63.9
MS.gene020466.t1 MTR_3g089005 36.538 104 62 3 1 104 24 123 1.98e-13 63.9
MS.gene020466.t1 MTR_4g130780 34.783 92 59 1 24 115 48 138 2.53e-13 65.1
MS.gene020466.t1 MTR_4g130800 35.514 107 63 3 5 106 24 129 4.50e-13 65.9
MS.gene020466.t1 MTR_7g086200 34.821 112 57 2 10 121 4 99 6.38e-13 62.8
MS.gene020466.t1 MTR_4g077787 30.882 136 79 4 12 135 34 166 6.72e-13 63.9
MS.gene020466.t1 MTR_8g094990 33.929 112 70 2 12 120 27 137 6.92e-13 65.1
MS.gene020466.t1 MTR_4g130800 40.244 82 48 1 25 106 48 128 9.00e-13 64.7
MS.gene020466.t1 MTR_4g124280 32.967 91 57 1 12 98 27 117 5.05e-12 62.4
MS.gene020466.t1 MTR_6g022170 37.000 100 55 4 12 106 36 132 5.96e-12 60.8
MS.gene020466.t1 MTR_2g090575 35.955 89 55 1 12 98 27 115 8.89e-12 62.8
MS.gene020466.t1 MTR_2g090580 25.000 172 119 3 12 173 29 200 1.07e-11 60.8
MS.gene020466.t1 MTR_6g013170 30.872 149 93 5 9 156 30 169 1.14e-11 60.5
MS.gene020466.t1 MTR_4g111640 28.931 159 95 5 12 164 34 180 1.34e-11 60.5
MS.gene020466.t1 MTR_3g105930 28.667 150 86 6 25 169 47 180 3.57e-11 59.3
MS.gene020466.t1 MTR_4g078410 25.974 154 111 2 12 163 27 179 4.22e-11 59.3
MS.gene020466.t1 MTR_1g014120 34.066 91 59 1 25 115 45 134 5.16e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020466.t1 AT1G72230 54.386 114 44 3 18 131 31 136 2.68e-34 119
MS.gene020466.t1 AT1G22480 55.670 97 41 2 16 112 25 119 1.57e-27 102
MS.gene020466.t1 AT5G07475 33.533 167 100 4 8 171 28 186 5.31e-26 98.6
MS.gene020466.t1 AT2G32300 40.909 132 71 3 5 131 21 150 1.57e-25 99.0
MS.gene020466.t1 AT2G32300 40.909 132 71 3 5 131 51 180 3.44e-25 98.6
MS.gene020466.t1 AT2G31050 45.299 117 60 3 7 122 26 139 1.38e-24 95.1
MS.gene020466.t1 AT3G17675 40.566 106 62 1 1 106 23 127 1.52e-23 90.5
MS.gene020466.t1 AT2G26720 40.351 114 66 2 7 119 26 138 2.47e-23 92.0
MS.gene020466.t1 AT5G26330 41.284 109 61 2 5 110 19 127 5.91e-23 90.5
MS.gene020466.t1 AT3G60270 47.959 98 49 2 12 109 28 123 3.69e-21 85.9
MS.gene020466.t1 AT3G27200 41.837 98 54 3 10 106 26 121 6.37e-19 79.7
MS.gene020466.t1 AT5G20230 36.471 170 97 4 12 173 27 193 1.70e-18 79.0
MS.gene020466.t1 AT2G44790 36.905 168 91 4 18 173 36 200 9.69e-17 74.7
MS.gene020466.t1 AT3G60280 42.857 98 54 2 12 109 26 121 8.59e-16 72.4
MS.gene020466.t1 AT1G64640 35.772 123 74 3 8 127 31 151 1.34e-14 68.6
MS.gene020466.t1 AT4G28365 29.480 173 111 4 5 169 24 193 5.57e-14 67.0
MS.gene020466.t1 AT2G25060 38.542 96 55 2 24 116 50 144 1.44e-13 65.9
MS.gene020466.t1 AT5G53870 30.909 110 74 1 12 119 32 141 2.20e-13 67.4
MS.gene020466.t1 AT5G53870 30.909 110 74 1 12 119 32 141 2.79e-13 67.0
MS.gene020466.t1 AT5G15350 33.333 132 79 5 1 127 17 144 3.13e-13 64.7
MS.gene020466.t1 AT2G02850 38.384 99 57 3 6 104 32 126 3.89e-13 63.5
MS.gene020466.t1 AT3G01070 33.333 117 77 1 12 127 29 145 1.92e-12 62.4
MS.gene020466.t1 AT4G27520 34.021 97 61 2 12 106 33 128 2.80e-12 63.9
MS.gene020466.t1 AT3G20570 30.380 158 99 3 12 159 32 188 5.85e-12 61.6
MS.gene020466.t1 AT4G31840 30.693 101 70 0 24 124 46 146 6.11e-12 61.2
MS.gene020466.t1 AT4G30590 32.520 123 77 4 9 126 27 148 7.94e-11 58.5

Find 40 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTCTCTGTTAAGGTTA+AGG 0.366793 3.3:+82258470 MS.gene020466:CDS
AGTAGCAATGGTGGTGGTGG+AGG 0.384189 3.3:-82258583 None:intergenic
CCTTCAGCAACGCCATCATC+AGG 0.386865 3.3:+82258513 MS.gene020466:CDS
CACGTTGTTGGAGATGCTTT+AGG 0.407015 3.3:+82258106 MS.gene020466:CDS
CACTATGGCATTGCCGACTC+AGG 0.423120 3.3:+82258075 None:intergenic
CAGGGAACTGTCCACGTTGT+TGG 0.443481 3.3:+82258094 MS.gene020466:CDS
AGGAGTAGCAATGGTGGTGG+TGG 0.467297 3.3:-82258586 None:intergenic
GGTGCAATAGTAATTGTGTT+GGG 0.490620 3.3:-82258561 None:intergenic
GGGAAAGGTTCACCTTCTAA+TGG 0.496016 3.3:+82258534 MS.gene020466:CDS
AGTGACAAAACCTTTGTAGT+TGG 0.507345 3.3:+82258163 MS.gene020466:CDS
TGCTGAAGGAGCAGCAGAAG+CGG 0.511020 3.3:-82258499 None:intergenic
TGGCATGAAACTCTCTGTTA+AGG 0.526108 3.3:+82258464 MS.gene020466:CDS
CCTGATGATGGCGTTGCTGA+AGG 0.526802 3.3:-82258513 None:intergenic
ACTAGTCTCTCATCAATCGT+TGG 0.529399 3.3:+82258630 MS.gene020466:CDS
GTTCCTGTACATTGCATAAG+TGG 0.532733 3.3:+82258441 MS.gene020466:CDS
ACAGTGTTCAATTATGAAGC+TGG 0.533391 3.3:+82258285 MS.gene020466:intron
GGTGAACCTTTCCCTGATGA+TGG 0.542469 3.3:-82258525 None:intergenic
TTTGTAGTTGGTGACAGTCT+CGG 0.547696 3.3:+82258175 MS.gene020466:CDS
CCACCACTTATGCAATGTAC+AGG 0.547760 3.3:-82258444 None:intergenic
GTAGCAATGGTGGTGGTGGA+GGG 0.552207 3.3:-82258582 None:intergenic
GTGCAATAGTAATTGTGTTG+GGG 0.560714 3.3:-82258560 None:intergenic
TGATTCTGATTATACAACAT+GGG 0.577703 3.3:+82258138 MS.gene020466:CDS
TTGTTGGAGATGCTTTAGGT+TGG 0.594901 3.3:+82258110 MS.gene020466:CDS
TTGATTCTGATTATACAACA+TGG 0.604668 3.3:+82258137 MS.gene020466:CDS
GGGTGCAATAGTAATTGTGT+TGG 0.604930 3.3:-82258562 None:intergenic
TTGCACGGTAGGAGTAGCAA+TGG 0.607518 3.3:-82258595 None:intergenic
ACTATGGCATTGCCGACTCA+GGG 0.610566 3.3:+82258076 None:intergenic
CACGGTAGGAGTAGCAATGG+TGG 0.625032 3.3:-82258592 None:intergenic
GCAACGCCATCATCAGGGAA+AGG 0.630930 3.3:+82258519 MS.gene020466:CDS
CAGTGTTCAATTATGAAGCT+GGG 0.634180 3.3:+82258286 MS.gene020466:intron
GGTAGGAGTAGCAATGGTGG+TGG 0.635365 3.3:-82258589 None:intergenic
TGCAATAGTAATTGTGTTGG+GGG 0.637451 3.3:-82258559 None:intergenic
ACGTGGACAGTTCCCTGAGT+CGG 0.637462 3.3:-82258088 None:intergenic
CTTCAGCAACGCCATCATCA+GGG 0.640963 3.3:+82258514 MS.gene020466:CDS
GAGACTGTCACCAACTACAA+AGG 0.655751 3.3:-82258173 None:intergenic
TCAATTAGTACAGATAGTAG+TGG 0.655860 3.3:+82258366 MS.gene020466:CDS
CCTGTACATTGCATAAGTGG+TGG 0.698977 3.3:+82258444 MS.gene020466:CDS
CTAAAGCATCTCCAACAACG+TGG 0.721869 3.3:-82258105 None:intergenic
TGAAGCTGGGTGGCACACAG+TGG 0.737276 3.3:+82258299 MS.gene020466:CDS
TGTTCAATTATGAAGCTGGG+TGG 0.745243 3.3:+82258289 MS.gene020466:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATTCTGATTATACAACAT+GGG + chr3.3:82258138-82258157 MS.gene020466:CDS 25.0%
! TTGATTCTGATTATACAACA+TGG + chr3.3:82258137-82258156 MS.gene020466:CDS 25.0%
TCAATTAGTACAGATAGTAG+TGG + chr3.3:82258366-82258385 MS.gene020466:CDS 30.0%
ACAGTGTTCAATTATGAAGC+TGG + chr3.3:82258285-82258304 MS.gene020466:intron 35.0%
AGTGACAAAACCTTTGTAGT+TGG + chr3.3:82258163-82258182 MS.gene020466:CDS 35.0%
CAGTGTTCAATTATGAAGCT+GGG + chr3.3:82258286-82258305 MS.gene020466:intron 35.0%
GAAACTCTCTGTTAAGGTTA+AGG + chr3.3:82258470-82258489 MS.gene020466:CDS 35.0%
GGTGCAATAGTAATTGTGTT+GGG - chr3.3:82258564-82258583 None:intergenic 35.0%
GTGCAATAGTAATTGTGTTG+GGG - chr3.3:82258563-82258582 None:intergenic 35.0%
TGCAATAGTAATTGTGTTGG+GGG - chr3.3:82258562-82258581 None:intergenic 35.0%
! AGTTGAAGATGCATTTTGCA+CGG - chr3.3:82258613-82258632 None:intergenic 35.0%
!!! AATGAGTTCCTACTTTTTCG+AGG - chr3.3:82258407-82258426 None:intergenic 35.0%
!!! ATGAGTTCCTACTTTTTCGA+GGG - chr3.3:82258406-82258425 None:intergenic 35.0%
GGGTGCAATAGTAATTGTGT+TGG - chr3.3:82258565-82258584 None:intergenic 40.0%
GTTCCTGTACATTGCATAAG+TGG + chr3.3:82258441-82258460 MS.gene020466:CDS 40.0%
TGGCATGAAACTCTCTGTTA+AGG + chr3.3:82258464-82258483 MS.gene020466:CDS 40.0%
TGTTCAATTATGAAGCTGGG+TGG + chr3.3:82258289-82258308 MS.gene020466:CDS 40.0%
! ACTAGTCTCTCATCAATCGT+TGG + chr3.3:82258630-82258649 MS.gene020466:CDS 40.0%
! TTGTTGGAGATGCTTTAGGT+TGG + chr3.3:82258110-82258129 MS.gene020466:CDS 40.0%
!! TTTGTAGTTGGTGACAGTCT+CGG + chr3.3:82258175-82258194 MS.gene020466:CDS 40.0%
!!! TGAGTTCCTACTTTTTCGAG+GGG - chr3.3:82258405-82258424 None:intergenic 40.0%
ACCATTCCCCTCGAAAAAGT+AGG + chr3.3:82258396-82258415 MS.gene020466:CDS 45.0%
CACGTTGTTGGAGATGCTTT+AGG + chr3.3:82258106-82258125 MS.gene020466:CDS 45.0%
CCACCACTTATGCAATGTAC+AGG - chr3.3:82258447-82258466 None:intergenic 45.0%
CCTGTACATTGCATAAGTGG+TGG + chr3.3:82258444-82258463 MS.gene020466:CDS 45.0%
GAGACTGTCACCAACTACAA+AGG - chr3.3:82258176-82258195 None:intergenic 45.0%
GGGAAAGGTTCACCTTCTAA+TGG + chr3.3:82258534-82258553 MS.gene020466:CDS 45.0%
! CTAAAGCATCTCCAACAACG+TGG - chr3.3:82258108-82258127 None:intergenic 45.0%
! GAAGATGCATTTTGCACGGT+AGG - chr3.3:82258609-82258628 None:intergenic 45.0%
!!! TCCTACTTTTTCGAGGGGAA+TGG - chr3.3:82258400-82258419 None:intergenic 45.0%
!!! TTTTTCGAGGGGAATGGTTG+TGG - chr3.3:82258394-82258413 None:intergenic 45.0%
CTTCAGCAACGCCATCATCA+GGG + chr3.3:82258514-82258533 MS.gene020466:CDS 50.0%
GGTGAACCTTTCCCTGATGA+TGG - chr3.3:82258528-82258547 None:intergenic 50.0%
TTGCACGGTAGGAGTAGCAA+TGG - chr3.3:82258598-82258617 None:intergenic 50.0%
ACGTGGACAGTTCCCTGAGT+CGG - chr3.3:82258091-82258110 None:intergenic 55.0%
CACGGTAGGAGTAGCAATGG+TGG - chr3.3:82258595-82258614 None:intergenic 55.0%
CAGGGAACTGTCCACGTTGT+TGG + chr3.3:82258094-82258113 MS.gene020466:CDS 55.0%
CCTTCAGCAACGCCATCATC+AGG + chr3.3:82258513-82258532 MS.gene020466:CDS 55.0%
GCAACGCCATCATCAGGGAA+AGG + chr3.3:82258519-82258538 MS.gene020466:CDS 55.0%
GGTAGGAGTAGCAATGGTGG+TGG - chr3.3:82258592-82258611 None:intergenic 55.0%
TGCTGAAGGAGCAGCAGAAG+CGG - chr3.3:82258502-82258521 None:intergenic 55.0%
!! AGGAGTAGCAATGGTGGTGG+TGG - chr3.3:82258589-82258608 None:intergenic 55.0%
!! AGTAGCAATGGTGGTGGTGG+AGG - chr3.3:82258586-82258605 None:intergenic 55.0%
!! CCTGATGATGGCGTTGCTGA+AGG - chr3.3:82258516-82258535 None:intergenic 55.0%
!! GTAGCAATGGTGGTGGTGGA+GGG - chr3.3:82258585-82258604 None:intergenic 55.0%
!! GTGTTGGGGGTGCCATTAGA+AGG - chr3.3:82258549-82258568 None:intergenic 55.0%
TGAAGCTGGGTGGCACACAG+TGG + chr3.3:82258299-82258318 MS.gene020466:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 82258079 82258695 82258079 ID=MS.gene020466
chr3.3 mRNA 82258079 82258695 82258079 ID=MS.gene020466.t1;Parent=MS.gene020466
chr3.3 exon 82258079 82258196 82258079 ID=MS.gene020466.t1.exon1;Parent=MS.gene020466.t1
chr3.3 CDS 82258079 82258196 82258079 ID=cds.MS.gene020466.t1;Parent=MS.gene020466.t1
chr3.3 exon 82258289 82258695 82258289 ID=MS.gene020466.t1.exon2;Parent=MS.gene020466.t1
chr3.3 CDS 82258289 82258695 82258289 ID=cds.MS.gene020466.t1;Parent=MS.gene020466.t1
Gene Sequence

>MS.gene020466

ATGGCATTGCCGACTCAGGGAACTGTCCACGTTGTTGGAGATGCTTTAGGTTGGACACTTGATTCTGATTATACAACATGGGCTAGTGACAAAACCTTTGTAGTTGGTGACAGTCTCGGTAAGTATCATAATTAATTGAAGTTTATCAATTCTATTGTTTGTTTAGTTTTACAATTTACAAAACTAATGTATGTAACTTTACTATGACAGTGTTCAATTATGAAGCTGGGTGGCACACAGTGGATGAAGTTAGAGAAAGTGACTATAAAACATGCACAACTAGAGATTCAATTAGTACAGATAGTAGTGGTGCCACAACCATTCCCCTCGAAAAAGTAGGAACTCATTACTTCATATGTGCTGTTCCTGTACATTGCATAAGTGGTGGCATGAAACTCTCTGTTAAGGTTAAGGCTTCTTCCGCTTCTGCTGCTCCTTCAGCAACGCCATCATCAGGGAAAGGTTCACCTTCTAATGGCACCCCCAACACAATTACTATTGCACCCTCCACCACCACCATTGCTACTCCTACCGTGCAAAATGCATCTTCAACTAGTCTCTCATCAATCGTTGGTTTATTTTTCGTTGTTATGTTGATCAGTTATATATTGATGTGA

Protein sequence

>MS.gene020466.t1

MALPTQGTVHVVGDALGWTLDSDYTTWASDKTFVVGDSLVFNYEAGWHTVDEVRESDYKTCTTRDSISTDSSGATTIPLEKVGTHYFICAVPVHCISGGMKLSVKVKASSASAAPSATPSSGKGSPSNGTPNTITIAPSTTTIATPTVQNASSTSLSSIVGLFFVVMLISYILM