AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020815

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020815.t1 MTR_5g096040 96.512 344 12 0 1 344 74 417 0.0 699
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020815.t1 AT5G63010 60.116 346 128 4 1 344 1 338 4.64e-153 434

Find 87 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCAGTTAATAAGGTATT+TGG 0.087837 5.4:-75369377 None:intergenic
TTTGCAATTGTTGCTATTAA+AGG 0.230758 5.4:+75369548 MS.gene020815:CDS
TGATATCCATCAGCCAAATT+TGG 0.233877 5.4:+75369226 MS.gene020815:CDS
GAACTCTAAGGTCCACAAAA+TGG 0.247499 5.4:+75369322 MS.gene020815:CDS
ATCATAGCTTCCAGTTAATA+AGG 0.268520 5.4:-75369384 None:intergenic
TAACTCTATGTGCTTGTATC+TGG 0.275517 5.4:+75369067 MS.gene020815:CDS
CTAATTGAAGTCTCATTAAC+AGG 0.277252 5.4:-75369446 None:intergenic
GACCCAAATACCTTATTAAC+TGG 0.308590 5.4:+75369374 MS.gene020815:CDS
CTTCATCCAAGGCCTTGTTT+TGG 0.318096 5.4:+75369505 MS.gene020815:CDS
GAGCCAGTATATACCAAATT+TGG 0.327127 5.4:-75369239 None:intergenic
TTAGCTTAGGTGGAGGAGTT+TGG 0.334473 5.4:+75369465 MS.gene020815:CDS
TTCTCTTCATGAATCCAATC+TGG 0.334547 5.4:+75369151 MS.gene020815:CDS
CTCTTCTTGTATTTCCAGAT+TGG 0.335556 5.4:-75369165 None:intergenic
TTCCAGTTAATAAGGTATTT+GGG 0.361076 5.4:-75369376 None:intergenic
CAGCCAAATTTGGTATATAC+TGG 0.372578 5.4:+75369236 MS.gene020815:CDS
CTTTCTATGACAAACTTGTC+AGG 0.398507 5.4:+75369693 MS.gene020815:CDS
GTAGCACATTGCTATTTAGA+AGG 0.402998 5.4:+75367605 MS.gene020815:CDS
TGAAATGCTTCCAGATCTAC+TGG 0.403251 5.4:-75367717 None:intergenic
TCTTGTATTTAAGAACTCTA+AGG 0.412316 5.4:+75369310 MS.gene020815:CDS
AGAGTTCTTAAATACAAGAT+TGG 0.418020 5.4:-75369306 None:intergenic
TTGGCTGCTTGCATGCATAA+TGG 0.422100 5.4:+75369524 MS.gene020815:CDS
CAAAACAAGGCCTTGGATGA+AGG 0.427193 5.4:-75369504 None:intergenic
AATGAGACTTCAATTAGCTT+AGG 0.435668 5.4:+75369452 MS.gene020815:CDS
ATGCTGGATGGTTGCTCTGA+TGG 0.442200 5.4:+75367890 MS.gene020815:CDS
GGTGACCAACCCAGTAGATC+TGG 0.445031 5.4:+75367707 MS.gene020815:CDS
AACTCTAAGGTCCACAAAAT+GGG 0.447471 5.4:+75369323 MS.gene020815:CDS
CTATGTGCTTGTATCTGGAT+TGG 0.464602 5.4:+75369072 MS.gene020815:CDS
ATGACAAACTTGTCAGGGTA+TGG 0.475765 5.4:+75369699 MS.gene020815:CDS
GCTTAGGTGGAGGAGTTTGG+AGG 0.486854 5.4:+75369468 MS.gene020815:CDS
ACCTTCTTGTAGTGTGTAAG+TGG 0.489791 5.4:-75367687 None:intergenic
AAGCACGATTCCCTTGCATA+TGG 0.490141 5.4:+75369602 MS.gene020815:CDS
CTTAGGTGGAGGAGTTTGGA+GGG 0.500724 5.4:+75369469 MS.gene020815:CDS
AAGCAGCCAAAACAAGGCCT+TGG 0.510827 5.4:-75369511 None:intergenic
TTCACTCTTTAATATTGAAG+CGG 0.521384 5.4:+75367736 MS.gene020815:CDS
TATATACCAAATTTGGCTGA+TGG 0.522943 5.4:-75369232 None:intergenic
AAAACAAGGCCTTGGATGAA+GGG 0.528136 5.4:-75369503 None:intergenic
AGGCGACAAAGCTGAAGTGT+TGG 0.538419 5.4:+75369568 MS.gene020815:CDS
AAAGAGCAAGTAGCAACTAC+TGG 0.540443 5.4:-75369674 None:intergenic
GCCAGTCTGCTCCATATGCA+AGG 0.541177 5.4:-75369613 None:intergenic
ATAAAGTGGAATCCACCTGC+AGG 0.543383 5.4:+75367812 MS.gene020815:CDS
GAGACTTCAATTAGCTTAGG+TGG 0.547687 5.4:+75369455 MS.gene020815:CDS
TTTAATATTGAAGCGGACAC+AGG 0.552003 5.4:+75367743 MS.gene020815:CDS
TCATCCACATCCATCACAGT+AGG 0.553375 5.4:+75369101 MS.gene020815:CDS
AAAGGACTTATGTTCCCTGC+AGG 0.554338 5.4:-75367827 None:intergenic
GGACTTATGTTCCCTGCAGG+TGG 0.557853 5.4:-75367824 None:intergenic
TTCTTAAATACAAGATTGGA+AGG 0.558501 5.4:-75369302 None:intergenic
TTAGGTCAAGCAGATGCTGA+TGG 0.559666 5.4:+75367851 MS.gene020815:CDS
GGTTGCTCTGATGGAATACA+AGG 0.562141 5.4:+75367899 MS.gene020815:CDS
ATGATGAATACTTGAGGGTG+TGG 0.565089 5.4:+75369402 MS.gene020815:CDS
ATCAGAAAGCCCTACTGTGA+TGG 0.567016 5.4:-75369111 None:intergenic
TACAAGATAATATCATTACA+TGG 0.572100 5.4:-75369725 None:intergenic
ATACTTGAGAATTAAGATGC+TGG 0.572344 5.4:+75367874 MS.gene020815:CDS
TATGGAGCAGACTGGCAGAA+AGG 0.573876 5.4:+75369620 MS.gene020815:CDS
ACATTGTGGTGCCCATTATG+TGG 0.577351 5.4:-75367653 None:intergenic
ATCTGGAAATACAAGAAGAG+TGG 0.579291 5.4:+75369168 MS.gene020815:CDS
TTTCTATGACAAACTTGTCA+GGG 0.580064 5.4:+75369694 MS.gene020815:CDS
TGCTTCCAGATCTACTGGGT+TGG 0.580660 5.4:-75367712 None:intergenic
GCATGCAAGCAGCCAAAACA+AGG 0.587727 5.4:-75369517 None:intergenic
TTTATAGTGAGGAGACTAGT+GGG 0.588794 5.4:+75367780 MS.gene020815:CDS
AATAAGGTATTTGGGTCATG+AGG 0.593807 5.4:-75369368 None:intergenic
ACTTCAATTAGCTTAGGTGG+AGG 0.594290 5.4:+75369458 MS.gene020815:CDS
AAGCTATGATGAATACTTGA+GGG 0.595787 5.4:+75369397 MS.gene020815:CDS
AAGCCCTACTGTGATGGATG+TGG 0.596404 5.4:-75369105 None:intergenic
TGGCTGATGGATATCAAATG+AGG 0.602475 5.4:-75369219 None:intergenic
AAGCATCATCCCTTCATCCA+AGG 0.604040 5.4:+75369494 MS.gene020815:CDS
GGAAATACAAGAAGAGTGGA+AGG 0.610010 5.4:+75369172 MS.gene020815:CDS
TCCACTTACACACTACAAGA+AGG 0.610467 5.4:+75367686 MS.gene020815:CDS
GAGAAGTAAATCACATGGGA+GGG 0.611106 5.4:+75369642 MS.gene020815:CDS
GAAGCTATGATGAATACTTG+AGG 0.614708 5.4:+75369396 MS.gene020815:CDS
CCAGTCTGCTCCATATGCAA+GGG 0.615600 5.4:-75369612 None:intergenic
CATCCACATCCATCACAGTA+GGG 0.616744 5.4:+75369102 MS.gene020815:CDS
AGAAGGTAATGCAGATGCTG+TGG 0.618373 5.4:+75367622 MS.gene020815:CDS
ACTGTGATGGATGTGGATGA+AGG 0.619011 5.4:-75369098 None:intergenic
CCCTTGCATATGGAGCAGAC+TGG 0.623412 5.4:+75369612 MS.gene020815:CDS
GGAGAAGTAAATCACATGGG+AGG 0.623635 5.4:+75369641 MS.gene020815:CDS
ACAAACTTGTCAGGGTATGG+AGG 0.625101 5.4:+75369702 MS.gene020815:CDS
ATCACAGTAGGGCTTTCTGA+TGG 0.627183 5.4:+75369113 MS.gene020815:CDS
ACAACAAAACTTGCAATCGT+CGG 0.634703 5.4:-75369261 None:intergenic
GTTTATAGTGAGGAGACTAG+TGG 0.636171 5.4:+75367779 MS.gene020815:CDS
TTGAGAATTAAGATGCTGGA+TGG 0.643048 5.4:+75367878 MS.gene020815:CDS
TGATGAATACTTGAGGGTGT+GGG 0.648470 5.4:+75369403 MS.gene020815:CDS
TGGATACAGCTAAAACATTG+TGG 0.659617 5.4:-75367667 None:intergenic
TAAAGTGGAATCCACCTGCA+GGG 0.661286 5.4:+75367813 MS.gene020815:CDS
GCTTGACACAGTTTATAGTG+AGG 0.668101 5.4:+75367769 MS.gene020815:CDS
GAAAGGAGAAGTAAATCACA+TGG 0.686537 5.4:+75369637 MS.gene020815:CDS
GAAATGCTTCCAGATCTACT+GGG 0.705745 5.4:-75367716 None:intergenic
AAAGGAGAAGTAAATCACAT+GGG 0.735171 5.4:+75369638 MS.gene020815:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATACAATTAAAAATAAT+AGG - chr5.4:75368730-75368749 None:intergenic 10.0%
!! AAGTCTAGAAATAAATTATA+CGG + chr5.4:75368001-75368020 MS.gene020815:intron 15.0%
!! AATAAAAAACAAACTAGAAA+TGG - chr5.4:75368529-75368548 None:intergenic 15.0%
!!! TTCTAGTTTGTTTTTTATTT+TGG + chr5.4:75368530-75368549 MS.gene020815:intron 15.0%
!!! TTTTAATTGTATCAAAGATT+TGG + chr5.4:75368735-75368754 MS.gene020815:intron 15.0%
!! AAACTCAATTATATAAGAGA+TGG - chr5.4:75368663-75368682 None:intergenic 20.0%
!! AAGGTAGTTATAAATTTCAT+AGG - chr5.4:75368693-75368712 None:intergenic 20.0%
!! AATTATATAAGAGATGGTAA+AGG - chr5.4:75368657-75368676 None:intergenic 20.0%
!! ACTATAGCATTATAATAAAG+CGG + chr5.4:75368143-75368162 MS.gene020815:intron 20.0%
!! AGGTAGTTATAAATTTCATA+GGG - chr5.4:75368692-75368711 None:intergenic 20.0%
!! CTAAATTGTTATGAATTTGA+TGG + chr5.4:75369004-75369023 MS.gene020815:intron 20.0%
!! GTTAAAAATATGTAAAAGTG+AGG - chr5.4:75368317-75368336 None:intergenic 20.0%
!! TACAAGATAATATCATTACA+TGG - chr5.4:75369728-75369747 None:intergenic 20.0%
!! TTTAGAAATGAAAGAATCAA+GGG - chr5.4:75367938-75367957 None:intergenic 20.0%
!!! TTCTAAAACACTATTTTCAT+TGG + chr5.4:75367951-75367970 MS.gene020815:intron 20.0%
!!! TTTTAGAAATGAAAGAATCA+AGG - chr5.4:75367939-75367958 None:intergenic 20.0%
! AAATATAGTATAGCTCTTCT+AGG + chr5.4:75368602-75368621 MS.gene020815:intron 25.0%
! AAATATGTAAAAGTGAGGTT+TGG - chr5.4:75368312-75368331 None:intergenic 25.0%
! AAGATTAACTGTAATGTAGA+AGG - chr5.4:75368868-75368887 None:intergenic 25.0%
! AATAAAATGTAACTCAGCAA+AGG - chr5.4:75368980-75368999 None:intergenic 25.0%
! AATATAGTATAGCTCTTCTA+GGG + chr5.4:75368603-75368622 MS.gene020815:intron 25.0%
! AGAGTTCTTAAATACAAGAT+TGG - chr5.4:75369309-75369328 None:intergenic 25.0%
! ATATATATCTTATAGCTAGC+TGG + chr5.4:75368776-75368795 MS.gene020815:intron 25.0%
! GGTAGTTATAAATTTCATAG+GGG - chr5.4:75368691-75368710 None:intergenic 25.0%
! GTAGTTATAAATTTCATAGG+GGG - chr5.4:75368690-75368709 None:intergenic 25.0%
! TACAAAGAAACATTAATACG+TGG - chr5.4:75368904-75368923 None:intergenic 25.0%
! TCTTGTATTTAAGAACTCTA+AGG + chr5.4:75369310-75369329 MS.gene020815:CDS 25.0%
! TTCACTCTTTAATATTGAAG+CGG + chr5.4:75367736-75367755 MS.gene020815:CDS 25.0%
! TTCTTAAATACAAGATTGGA+AGG - chr5.4:75369305-75369324 None:intergenic 25.0%
!! ACTATTGAATTTACGTCTTT+TGG - chr5.4:75368257-75368276 None:intergenic 25.0%
!! ATAACTACCTTCTAATTTGT+TGG + chr5.4:75368702-75368721 MS.gene020815:intron 25.0%
!! TAGAAGGTTGTATTTTGTAA+TGG - chr5.4:75368852-75368871 None:intergenic 25.0%
!! TTCCAGTTAATAAGGTATTT+GGG - chr5.4:75369379-75369398 None:intergenic 25.0%
!! TTTGCAATTGTTGCTATTAA+AGG + chr5.4:75369548-75369567 MS.gene020815:CDS 25.0%
AAAGGAGAAGTAAATCACAT+GGG + chr5.4:75369638-75369657 MS.gene020815:CDS 30.0%
AAGAAACATTAATACGTGGA+AGG - chr5.4:75368900-75368919 None:intergenic 30.0%
AAGCTATGATGAATACTTGA+GGG + chr5.4:75369397-75369416 MS.gene020815:CDS 30.0%
ATACTTGAGAATTAAGATGC+TGG + chr5.4:75367874-75367893 MS.gene020815:CDS 30.0%
ATCATAGCTTCCAGTTAATA+AGG - chr5.4:75369387-75369406 None:intergenic 30.0%
CTAATTGAAGTCTCATTAAC+AGG - chr5.4:75369449-75369468 None:intergenic 30.0%
TATATACCAAATTTGGCTGA+TGG - chr5.4:75369235-75369254 None:intergenic 30.0%
TCATATATCAGTGTCATGAT+GGG - chr5.4:75368353-75368372 None:intergenic 30.0%
TTCCACTGCAAATTTATCAA+GGG - chr5.4:75368220-75368239 None:intergenic 30.0%
TTTCCACTGCAAATTTATCA+AGG - chr5.4:75368221-75368240 None:intergenic 30.0%
TTTCTATGACAAACTTGTCA+GGG + chr5.4:75369694-75369713 MS.gene020815:CDS 30.0%
! AATGAGACTTCAATTAGCTT+AGG + chr5.4:75369452-75369471 MS.gene020815:CDS 30.0%
! AGGAATGCAATATTTTTCCA+TGG - chr5.4:75368960-75368979 None:intergenic 30.0%
! ATTGTTATGAATTTGATGGC+AGG + chr5.4:75369008-75369027 MS.gene020815:intron 30.0%
! CTTCCAGTTAATAAGGTATT+TGG - chr5.4:75369380-75369399 None:intergenic 30.0%
! TTGTTATGAATTTGATGGCA+GGG + chr5.4:75369009-75369028 MS.gene020815:intron 30.0%
!!! TTTTTTTTTCCCCGATTTCT+TGG + chr5.4:75368404-75368423 MS.gene020815:intron 30.0%
AATTATACGGTACTTACCCT+CGG + chr5.4:75368014-75368033 MS.gene020815:intron 35.0%
ACAACAAAACTTGCAATCGT+CGG - chr5.4:75369264-75369283 None:intergenic 35.0%
ATAGGCACCAACAAATTAGA+AGG - chr5.4:75368712-75368731 None:intergenic 35.0%
ATCTGGAAATACAAGAAGAG+TGG + chr5.4:75369168-75369187 MS.gene020815:CDS 35.0%
ATGGCAAGAATGAATAACAG+AGG + chr5.4:75368456-75368475 MS.gene020815:intron 35.0%
CAGCCAAATTTGGTATATAC+TGG + chr5.4:75369236-75369255 MS.gene020815:CDS 35.0%
CTCATATATCAGTGTCATGA+TGG - chr5.4:75368354-75368373 None:intergenic 35.0%
CTCTTCTTGTATTTCCAGAT+TGG - chr5.4:75369168-75369187 None:intergenic 35.0%
CTTTCTATGACAAACTTGTC+AGG + chr5.4:75369693-75369712 MS.gene020815:CDS 35.0%
GAAAGGAGAAGTAAATCACA+TGG + chr5.4:75369637-75369656 MS.gene020815:CDS 35.0%
GAAGCTATGATGAATACTTG+AGG + chr5.4:75369396-75369415 MS.gene020815:CDS 35.0%
GAATAACAGAGGAGTTTGAT+TGG + chr5.4:75368467-75368486 MS.gene020815:intron 35.0%
GACCCAAATACCTTATTAAC+TGG + chr5.4:75369374-75369393 MS.gene020815:CDS 35.0%
GAGCCAGTATATACCAAATT+TGG - chr5.4:75369242-75369261 None:intergenic 35.0%
TAACTCTATGTGCTTGTATC+TGG + chr5.4:75369067-75369086 MS.gene020815:CDS 35.0%
TACCCTTGATAAATTTGCAG+TGG + chr5.4:75368215-75368234 MS.gene020815:intron 35.0%
TGATATCCATCAGCCAAATT+TGG + chr5.4:75369226-75369245 MS.gene020815:CDS 35.0%
TGGATACAGCTAAAACATTG+TGG - chr5.4:75367670-75367689 None:intergenic 35.0%
TTACAGATCGCAGAAAATAG+CGG - chr5.4:75368181-75368200 None:intergenic 35.0%
TTCTCTTCATGAATCCAATC+TGG + chr5.4:75369151-75369170 MS.gene020815:CDS 35.0%
TTGAGAATTAAGATGCTGGA+TGG + chr5.4:75367878-75367897 MS.gene020815:CDS 35.0%
TTTAATATTGAAGCGGACAC+AGG + chr5.4:75367743-75367762 MS.gene020815:CDS 35.0%
TTTATAGTGAGGAGACTAGT+GGG + chr5.4:75367780-75367799 MS.gene020815:CDS 35.0%
! AATAAGGTATTTGGGTCATG+AGG - chr5.4:75369371-75369390 None:intergenic 35.0%
! AGGCACATGATTTTGAACTT+TGG + chr5.4:75369192-75369211 MS.gene020815:CDS 35.0%
! ATGGTTCAGTATGTGTTTTG+CGG - chr5.4:75368510-75368529 None:intergenic 35.0%
! ATTTTCCTTCCAAGAAATCG+GGG - chr5.4:75368416-75368435 None:intergenic 35.0%
! CATTTTCCTTCCAAGAAATC+GGG - chr5.4:75368417-75368436 None:intergenic 35.0%
! GCATTTTCCTTCCAAGAAAT+CGG - chr5.4:75368418-75368437 None:intergenic 35.0%
! GTGGGATTTTCGATATAAAG+TGG + chr5.4:75367798-75367817 MS.gene020815:CDS 35.0%
! TGCAATATTTTTCCATGGCA+AGG - chr5.4:75368955-75368974 None:intergenic 35.0%
! TGTTATGAATTTGATGGCAG+GGG + chr5.4:75369010-75369029 MS.gene020815:intron 35.0%
! TTGTTGATTAGCAGCTATTG+TGG + chr5.4:75368119-75368138 MS.gene020815:intron 35.0%
! TTTGGTTTCTCTACATGCAA+AGG + chr5.4:75368753-75368772 MS.gene020815:intron 35.0%
!! AACTCTAAGGTCCACAAAAT+GGG + chr5.4:75369323-75369342 MS.gene020815:CDS 35.0%
!! GTAGCACATTGCTATTTAGA+AGG + chr5.4:75367605-75367624 MS.gene020815:CDS 35.0%
!! TGGAGTTTTGTCCACATAAT+GGG + chr5.4:75367642-75367661 MS.gene020815:CDS 35.0%
!!! ACGATTGCAAGTTTTGTTGT+TGG + chr5.4:75369264-75369283 MS.gene020815:CDS 35.0%
!!! AGTTTTGTTGTTGGGATTTG+CGG + chr5.4:75369273-75369292 MS.gene020815:CDS 35.0%
!!! CGATTGCAAGTTTTGTTGTT+GGG + chr5.4:75369265-75369284 MS.gene020815:CDS 35.0%
!!! GGGAACATAAGTCCTTTTTT+AGG + chr5.4:75367833-75367852 MS.gene020815:CDS 35.0%
AAAACAAGGCCTTGGATGAA+GGG - chr5.4:75369506-75369525 None:intergenic 40.0%
AAAGAGCAAGTAGCAACTAC+TGG - chr5.4:75369677-75369696 None:intergenic 40.0%
ACCTTCTTGTAGTGTGTAAG+TGG - chr5.4:75367690-75367709 None:intergenic 40.0%
ATGACAAACTTGTCAGGGTA+TGG + chr5.4:75369699-75369718 MS.gene020815:CDS 40.0%
ATGATGAATACTTGAGGGTG+TGG + chr5.4:75369402-75369421 MS.gene020815:CDS 40.0%
CCGTTATTTGACAAAACCGA+GGG - chr5.4:75368033-75368052 None:intergenic 40.0%
CTATGTGCTTGTATCTGGAT+TGG + chr5.4:75369072-75369091 MS.gene020815:CDS 40.0%
CTCTGATGGAATACAAGGTA+TGG + chr5.4:75367904-75367923 MS.gene020815:intron 40.0%
CTGAACATTACGAACAGTGA+TGG + chr5.4:75368437-75368456 MS.gene020815:intron 40.0%
GAAATGCTTCCAGATCTACT+GGG - chr5.4:75367719-75367738 None:intergenic 40.0%
GAGAAGTAAATCACATGGGA+GGG + chr5.4:75369642-75369661 MS.gene020815:CDS 40.0%
GATAAAAGCAGTGACATGTG+CGG + chr5.4:75368924-75368943 MS.gene020815:intron 40.0%
GCATCTGCTTGACCTAAAAA+AGG - chr5.4:75367848-75367867 None:intergenic 40.0%
GCTTGACACAGTTTATAGTG+AGG + chr5.4:75367769-75367788 MS.gene020815:CDS 40.0%
GGAAATACAAGAAGAGTGGA+AGG + chr5.4:75369172-75369191 MS.gene020815:CDS 40.0%
GTTTATAGTGAGGAGACTAG+TGG + chr5.4:75367779-75367798 MS.gene020815:CDS 40.0%
TCCACTTACACACTACAAGA+AGG + chr5.4:75367686-75367705 MS.gene020815:CDS 40.0%
TGAAATGCTTCCAGATCTAC+TGG - chr5.4:75367720-75367739 None:intergenic 40.0%
TGTCTGCAAGAATGCATACA+TGG - chr5.4:75368563-75368582 None:intergenic 40.0%
! ACTTCAATTAGCTTAGGTGG+AGG + chr5.4:75369458-75369477 MS.gene020815:CDS 40.0%
! GAGACTTCAATTAGCTTAGG+TGG + chr5.4:75369455-75369474 MS.gene020815:CDS 40.0%
! TGATGAATACTTGAGGGTGT+GGG + chr5.4:75369403-75369422 MS.gene020815:CDS 40.0%
! TGCAAGTAACACCCATTTTG+TGG - chr5.4:75369337-75369356 None:intergenic 40.0%
! TGGCTGATGGATATCAAATG+AGG - chr5.4:75369222-75369241 None:intergenic 40.0%
! TGGTTCAGTATGTGTTTTGC+GGG - chr5.4:75368509-75368528 None:intergenic 40.0%
! TTTTTCCCCGATTTCTTGGA+AGG + chr5.4:75368408-75368427 MS.gene020815:intron 40.0%
!! CCCTCGGTTTTGTCAAATAA+CGG + chr5.4:75368030-75368049 MS.gene020815:intron 40.0%
!! GAACTCTAAGGTCCACAAAA+TGG + chr5.4:75369322-75369341 MS.gene020815:CDS 40.0%
!! GTGGAGTTTTGTCCACATAA+TGG + chr5.4:75367641-75367660 MS.gene020815:CDS 40.0%
!!! GTTTTGTTGTTGGGATTTGC+GGG + chr5.4:75369274-75369293 MS.gene020815:CDS 40.0%
AAAGGACTTATGTTCCCTGC+AGG - chr5.4:75367830-75367849 None:intergenic 45.0%
AAGCACGATTCCCTTGCATA+TGG + chr5.4:75369602-75369621 MS.gene020815:CDS 45.0%
AAGCATCATCCCTTCATCCA+AGG + chr5.4:75369494-75369513 MS.gene020815:CDS 45.0%
ACAAACTTGTCAGGGTATGG+AGG + chr5.4:75369702-75369721 MS.gene020815:CDS 45.0%
ACATTGTGGTGCCCATTATG+TGG - chr5.4:75367656-75367675 None:intergenic 45.0%
AGAAGGTAATGCAGATGCTG+TGG + chr5.4:75367622-75367641 MS.gene020815:CDS 45.0%
AGCTAGCTGGATATGCACTT+GGG + chr5.4:75368789-75368808 MS.gene020815:intron 45.0%
ATAAAGTGGAATCCACCTGC+AGG + chr5.4:75367812-75367831 MS.gene020815:CDS 45.0%
ATAACGGCGCTATAGCATAG+CGG + chr5.4:75368046-75368065 MS.gene020815:intron 45.0%
ATCACAGTAGGGCTTTCTGA+TGG + chr5.4:75369113-75369132 MS.gene020815:CDS 45.0%
ATCAGAAAGCCCTACTGTGA+TGG - chr5.4:75369114-75369133 None:intergenic 45.0%
CAAAACAAGGCCTTGGATGA+AGG - chr5.4:75369507-75369526 None:intergenic 45.0%
CATCCACATCCATCACAGTA+GGG + chr5.4:75369102-75369121 MS.gene020815:CDS 45.0%
GCCGTTATTTGACAAAACCG+AGG - chr5.4:75368034-75368053 None:intergenic 45.0%
GGAGAAGTAAATCACATGGG+AGG + chr5.4:75369641-75369660 MS.gene020815:CDS 45.0%
GGCATTTCCTACTCTCACAA+CGG - chr5.4:75368291-75368310 None:intergenic 45.0%
GGTTGCTCTGATGGAATACA+AGG + chr5.4:75367899-75367918 MS.gene020815:CDS 45.0%
TAAAGTGGAATCCACCTGCA+GGG + chr5.4:75367813-75367832 MS.gene020815:CDS 45.0%
TAGCTAGCTGGATATGCACT+TGG + chr5.4:75368788-75368807 MS.gene020815:intron 45.0%
TCATCCACATCCATCACAGT+AGG + chr5.4:75369101-75369120 MS.gene020815:CDS 45.0%
TTAGCTTAGGTGGAGGAGTT+TGG + chr5.4:75369465-75369484 MS.gene020815:CDS 45.0%
TTAGGTCAAGCAGATGCTGA+TGG + chr5.4:75367851-75367870 MS.gene020815:CDS 45.0%
TTGGCTGCTTGCATGCATAA+TGG + chr5.4:75369524-75369543 MS.gene020815:CDS 45.0%
! ATGTGTTTTGCGGGAATCAC+AGG - chr5.4:75368500-75368519 None:intergenic 45.0%
!! ACTGTGATGGATGTGGATGA+AGG - chr5.4:75369101-75369120 None:intergenic 45.0%
!! CAGTGTCATGATGGGTTTCT+AGG - chr5.4:75368345-75368364 None:intergenic 45.0%
!!! CTTCATCCAAGGCCTTGTTT+TGG + chr5.4:75369505-75369524 MS.gene020815:CDS 45.0%
!!! TTACGTCTTTTGGAGCTCTG+TGG - chr5.4:75368247-75368266 None:intergenic 45.0%
AACTGCTCCGTTGTGAGAGT+AGG + chr5.4:75368281-75368300 MS.gene020815:intron 50.0%
AAGCAGCCAAAACAAGGCCT+TGG - chr5.4:75369514-75369533 None:intergenic 50.0%
AGGCGACAAAGCTGAAGTGT+TGG + chr5.4:75369568-75369587 MS.gene020815:CDS 50.0%
ATGCTGGATGGTTGCTCTGA+TGG + chr5.4:75367890-75367909 MS.gene020815:CDS 50.0%
CCAGTCTGCTCCATATGCAA+GGG - chr5.4:75369615-75369634 None:intergenic 50.0%
CTTAGGTGGAGGAGTTTGGA+GGG + chr5.4:75369469-75369488 MS.gene020815:CDS 50.0%
GCATGCAAGCAGCCAAAACA+AGG - chr5.4:75369520-75369539 None:intergenic 50.0%
TATGGAGCAGACTGGCAGAA+AGG + chr5.4:75369620-75369639 MS.gene020815:CDS 50.0%
TGCTTCCAGATCTACTGGGT+TGG - chr5.4:75367715-75367734 None:intergenic 50.0%
! ACTTGGGCACTTTTCTCAGG+AGG + chr5.4:75368805-75368824 MS.gene020815:intron 50.0%
! GGCACTTTTCTCAGGAGGAT+TGG + chr5.4:75368810-75368829 MS.gene020815:intron 50.0%
! TGCACTTGGGCACTTTTCTC+AGG + chr5.4:75368802-75368821 MS.gene020815:intron 50.0%
!! AAGCCCTACTGTGATGGATG+TGG - chr5.4:75369108-75369127 None:intergenic 50.0%
CCCTTGCATATGGAGCAGAC+TGG + chr5.4:75369612-75369631 MS.gene020815:CDS 55.0%
GCCAGTCTGCTCCATATGCA+AGG - chr5.4:75369616-75369635 None:intergenic 55.0%
GCTTAGGTGGAGGAGTTTGG+AGG + chr5.4:75369468-75369487 MS.gene020815:CDS 55.0%
GGACTTATGTTCCCTGCAGG+TGG - chr5.4:75367827-75367846 None:intergenic 55.0%
GGTGACCAACCCAGTAGATC+TGG + chr5.4:75367707-75367726 MS.gene020815:CDS 55.0%
TGTGCGGATGTGCCTTGCCA+TGG + chr5.4:75368940-75368959 MS.gene020815:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 75367599 75369748 75367599 ID=MS.gene020815
chr5.4 mRNA 75367599 75369748 75367599 ID=MS.gene020815.t1;Parent=MS.gene020815
chr5.4 exon 75367599 75367920 75367599 ID=MS.gene020815.t1.exon1;Parent=MS.gene020815.t1
chr5.4 CDS 75367599 75367920 75367599 ID=cds.MS.gene020815.t1;Parent=MS.gene020815.t1
chr5.4 exon 75369030 75369748 75369030 ID=MS.gene020815.t1.exon2;Parent=MS.gene020815.t1
chr5.4 CDS 75369030 75369748 75369030 ID=cds.MS.gene020815.t1;Parent=MS.gene020815.t1
Gene Sequence

>MS.gene020815

ATGGATGTAGCACATTGCTATTTAGAAGGTAATGCAGATGCTGTGGAGTTTTGTCCACATAATGGGCACCACAATGTTTTAGCTGTATCCACTTACACACTACAAGAAGGTGACCAACCCAGTAGATCTGGAAGCATTTCACTCTTTAATATTGAAGCGGACACAGGCAAGCTTGACACAGTTTATAGTGAGGAGACTAGTGGGATTTTCGATATAAAGTGGAATCCACCTGCAGGGAACATAAGTCCTTTTTTAGGTCAAGCAGATGCTGATGGATACTTGAGAATTAAGATGCTGGATGGTTGCTCTGATGGAATACAAGGTATGGATTTTTATCCCTTGATTCTTTCATTTCTAAAACACTATTTTCATTGGTGAGTACAACTACAAGCGTGATAGTAGAAGTCTAGAAATAAATTATACGGTACTTACCCTCGGTTTTGTCAAATAACGGCGCTATAGCATAGCGGAATTTAAACAAATCGATATTATTCTGCAATACGCAATTTAGTACAAAGTGTTGTTGATTAGCAGCTATTGTGGCACTATAGCATTATAATAAAGCGGAATTTGAACAAACCGCTATTTTCTGCGATCTGTAATTGACAACACTGCTTACCCTTGATAAATTTGCAGTGGAAAGCACCACAGAGCTCCAAAAGACGTAAATTCAATAGTAGTTAACTGCTCCGTTGTGAGAGTAGGAAATGCCAAACCTCACTTTTACATATTTTTAACACAGTCCTAGAAACCCATCATGACACTGATATATGAGTTATGACATCTTTATATCATGTTTGTTCTTTTTTTTTTTCCCCGATTTCTTGGAAGGAAAATGCTGAACATTACGAACAGTGATGGCAAGAATGAATAACAGAGGAGTTTGATTGGCTACTCACCTGTGATTCCCGCAAAACACATACTGAACCATTTCTAGTTTGTTTTTTATTTTGGAAAACTGCCATGTATGCATTCTTGCAGACATAGACAAATTTCTTTTATTAAATATAGTATAGCTCTTCTAGGGATATAACTAGTTTTTCTTTTTTTTTTAACCTTTACCATCTCTTATATAATTGAGTTTTTTTCCCCCTATGAAATTTATAACTACCTTCTAATTTGTTGGTGCCTATTATTTTTAATTGTATCAAAGATTTGGTTTCTCTACATGCAAAGGATATATATCTTATAGCTAGCTGGATATGCACTTGGGCACTTTTCTCAGGAGGATTGGTTGTTTATTTAAATATCCATTACAAAATACAACCTTCTACATTACAGTTAATCTTTAAGCATTACCTTCCACGTATTAATGTTTCTTTGTAGATAAAAGCAGTGACATGTGCGGATGTGCCTTGCCATGGAAAAATATTGCATTCCTTTGCTGAGTTACATTTTATTAAAACTAAATTGTTATGAATTTGATGGCAGGGGCTAATCTAAAAGAGATTACAAGTGAGAAAGTCAGTAACTCTATGTGCTTGTATCTGGATTGGAATCCTTCATCCACATCCATCACAGTAGGGCTTTCTGATGGCAGTGTTTCTATTGTTTCTCTTCATGAATCCAATCTGGAAATACAAGAAGAGTGGAAGGCACATGATTTTGAACTTTGGACAACCTCATTTGATATCCATCAGCCAAATTTGGTATATACTGGCTCCGACGATTGCAAGTTTTGTTGTTGGGATTTGCGGGACAGTCCTTCCAATCTTGTATTTAAGAACTCTAAGGTCCACAAAATGGGTGTTACTTGCATTGAAAAGAGTCCTCATGACCCAAATACCTTATTAACTGGAAGCTATGATGAATACTTGAGGGTGTGGGATTTAAGATCAATCTCAAAACCTGTTAATGAGACTTCAATTAGCTTAGGTGGAGGAGTTTGGAGGGTGAAGCATCATCCCTTCATCCAAGGCCTTGTTTTGGCTGCTTGCATGCATAATGGTTTTGCAATTGTTGCTATTAAAGGCGACAAAGCTGAAGTGTTGGAAACGTACAAGAAGCACGATTCCCTTGCATATGGAGCAGACTGGCAGAAAGGAGAAGTAAATCACATGGGAGGGAGCAGTAAACCAGTAGTTGCTACTTGCTCTTTCTATGACAAACTTGTCAGGGTATGGAGGCCATGTAATGATATTATCTTGTAA
Protein sequence

>MS.gene020815.t1

MDVAHCYLEGNADAVEFCPHNGHHNVLAVSTYTLQEGDQPSRSGSISLFNIEADTGKLDTVYSEETSGIFDIKWNPPAGNISPFLGQADADGYLRIKMLDGCSDGIQGANLKEITSEKVSNSMCLYLDWNPSSTSITVGLSDGSVSIVSLHESNLEIQEEWKAHDFELWTTSFDIHQPNLVYTGSDDCKFCCWDLRDSPSNLVFKNSKVHKMGVTCIEKSPHDPNTLLTGSYDEYLRVWDLRSISKPVNETSISLGGGVWRVKHHPFIQGLVLAACMHNGFAIVAIKGDKAEVLETYKKHDSLAYGADWQKGEVNHMGGSSKPVVATCSFYDKLVRVWRPCNDIIL