AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02239

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02239.t1 MTR_2g010450 94.618 353 19 0 1 353 1 353 0.0 695
MS.gene02239.t1 MTR_4g092700 55.587 349 111 10 7 353 27 333 5.25e-115 337
MS.gene02239.t1 MTR_4g097940 39.943 348 173 14 5 345 2 320 8.58e-59 193
MS.gene02239.t1 MTR_4g097920 39.943 348 173 14 5 345 2 320 8.84e-58 190
MS.gene02239.t1 MTR_5g014600 44.358 257 115 6 106 346 86 330 2.66e-57 189
MS.gene02239.t1 MTR_5g014520 52.632 190 87 2 166 353 144 332 6.83e-55 183
MS.gene02239.t1 MTR_4g097920 41.914 303 149 12 44 345 29 305 5.19e-53 177
MS.gene02239.t1 MTR_0250s0040 45.813 203 106 2 151 353 65 263 9.83e-51 170
MS.gene02239.t1 MTR_0246s0020 44.643 224 107 5 138 353 163 377 3.21e-49 169
MS.gene02239.t1 MTR_5g014640 34.104 346 200 10 5 348 2 321 6.36e-49 167
MS.gene02239.t1 MTR_4g097950 54.749 179 69 4 177 346 70 245 1.65e-48 164
MS.gene02239.t1 MTR_8g009020 42.081 221 116 4 134 346 102 318 2.84e-48 166
MS.gene02239.t1 MTR_6g047570 46.154 195 100 4 161 353 143 334 4.02e-47 163
MS.gene02239.t1 MTR_5g014560 42.793 222 117 4 131 348 86 301 1.62e-46 160
MS.gene02239.t1 MTR_2g104550 50.847 177 81 2 173 346 134 307 2.79e-46 160
MS.gene02239.t1 MTR_0011s0210 47.059 187 82 4 175 353 181 358 8.69e-46 160
MS.gene02239.t1 MTR_0011s0260 45.810 179 96 1 175 353 181 358 3.13e-45 159
MS.gene02239.t1 MTR_0246s0020 45.771 201 92 5 138 330 163 354 6.46e-44 155
MS.gene02239.t1 MTR_4g067010 43.684 190 101 2 166 353 134 319 5.95e-43 152
MS.gene02239.t1 MTR_4g098035 39.904 208 107 3 156 353 141 340 1.72e-41 148
MS.gene02239.t1 MTR_0246s0050 39.904 208 107 3 156 353 120 319 2.17e-41 147
MS.gene02239.t1 MTR_2g104490 46.927 179 92 2 173 351 133 308 1.86e-39 142
MS.gene02239.t1 MTR_4g009540 45.856 181 89 3 173 346 148 326 1.99e-39 142
MS.gene02239.t1 MTR_4g009110 46.067 178 90 2 173 346 170 345 2.20e-39 143
MS.gene02239.t1 MTR_4g066460 41.627 209 111 3 151 352 122 326 7.47e-39 141
MS.gene02239.t1 MTR_2g104560 42.697 178 89 5 174 346 51 220 8.45e-39 138
MS.gene02239.t1 MTR_4g066380 40.952 210 112 3 151 353 120 324 1.56e-37 137
MS.gene02239.t1 MTR_2g104490 46.386 166 86 2 173 338 133 295 2.06e-36 134
MS.gene02239.t1 MTR_2g104650 43.094 181 100 2 168 346 85 264 8.61e-36 131
MS.gene02239.t1 MTR_8g009010 40.230 174 66 2 174 346 131 267 2.90e-35 131
MS.gene02239.t1 MTR_4g009540 46.061 165 80 3 173 330 148 310 1.18e-34 129
MS.gene02239.t1 MTR_8g069740 43.636 165 88 4 168 329 103 265 7.63e-34 126
MS.gene02239.t1 MTR_2g104590 39.773 176 83 3 173 346 65 219 2.73e-32 121
MS.gene02239.t1 MTR_7g080780 41.860 172 90 1 175 346 150 311 3.91e-31 120
MS.gene02239.t1 MTR_7g080780 41.860 172 90 1 175 346 163 324 5.32e-31 120
MS.gene02239.t1 MTR_2g104530 38.150 173 94 3 178 346 106 269 1.22e-29 115
MS.gene02239.t1 MTR_2g104500 38.728 173 94 4 177 346 104 267 3.92e-29 114
MS.gene02239.t1 MTR_7g451650 38.816 152 82 2 200 351 57 197 1.53e-25 102
MS.gene02239.t1 MTR_4g009510 35.260 173 99 4 180 351 45 205 4.19e-24 98.6
MS.gene02239.t1 MTR_7g050350 37.671 146 80 2 206 351 1 135 7.43e-24 95.9
MS.gene02239.t1 MTR_7g451650 37.671 146 80 2 206 351 1 135 7.43e-24 95.9
MS.gene02239.t1 MTR_4g009420 37.500 152 76 2 200 351 65 197 2.40e-23 96.3
MS.gene02239.t1 MTR_5g014550 41.732 127 73 1 207 333 54 179 1.24e-22 94.4
MS.gene02239.t1 MTR_6g047550 44.262 122 62 3 130 249 127 244 6.92e-22 94.0
MS.gene02239.t1 MTR_7g451650 37.778 135 73 2 200 334 57 180 2.58e-21 90.5
MS.gene02239.t1 MTR_1g072320 34.395 157 93 3 177 324 470 625 7.85e-20 91.3
MS.gene02239.t1 MTR_4g009420 36.296 135 67 2 200 334 65 180 6.00e-19 84.3
MS.gene02239.t1 MTR_8g027495 32.353 136 83 2 177 312 458 584 3.72e-16 80.1
MS.gene02239.t1 MTR_7g117670 31.618 136 84 2 177 312 402 528 5.21e-16 79.7
MS.gene02239.t1 MTR_3g104100 29.032 155 101 2 179 324 197 351 1.53e-15 77.4
MS.gene02239.t1 MTR_8g067280 33.143 175 106 3 150 313 434 608 1.60e-15 78.2
MS.gene02239.t1 MTR_5g030430 31.333 150 87 3 177 313 493 639 5.84e-15 76.6
MS.gene02239.t1 MTR_3g030700 29.189 185 120 4 149 329 175 352 8.87e-15 75.1
MS.gene02239.t1 MTR_8g024790 32.168 143 85 3 177 313 291 427 1.11e-14 75.1
MS.gene02239.t1 MTR_3g498695 29.032 155 101 2 179 324 300 454 1.48e-14 75.1
MS.gene02239.t1 MTR_3g498695 29.032 155 101 2 179 324 300 454 1.57e-14 74.7
MS.gene02239.t1 MTR_7g096350 54.386 57 26 0 177 233 355 411 9.17e-14 72.4
MS.gene02239.t1 MTR_8g024770 27.711 166 99 3 177 330 279 435 1.75e-13 71.6
MS.gene02239.t1 MTR_7g083900 28.302 159 99 3 179 324 288 444 3.31e-13 70.9
MS.gene02239.t1 MTR_7g083900 28.302 159 99 3 179 324 288 444 3.47e-13 70.9
MS.gene02239.t1 MTR_2g081040 29.032 155 101 3 179 324 179 333 1.27e-12 68.6
MS.gene02239.t1 MTR_8g064700 25.843 178 110 5 166 327 227 398 1.45e-11 65.5
MS.gene02239.t1 MTR_8g098275 25.962 208 121 5 174 352 241 444 3.44e-11 64.7
MS.gene02239.t1 MTR_8g098275 25.962 208 121 5 174 352 305 508 5.16e-11 64.3
MS.gene02239.t1 MTR_8g098275 25.962 208 121 5 174 352 398 601 6.50e-11 63.9
MS.gene02239.t1 MTR_1g112680 38.710 93 53 2 168 257 309 400 6.58e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02239.t1 AT4G37850 56.757 185 72 4 173 353 148 328 9.18e-60 196
MS.gene02239.t1 AT4G37850 56.757 185 72 4 173 353 148 328 9.18e-60 196
MS.gene02239.t1 AT2G22760 49.153 177 87 3 175 348 117 293 1.12e-48 166
MS.gene02239.t1 AT2G22770 46.809 188 86 2 177 350 132 319 7.02e-45 157
MS.gene02239.t1 AT2G22750 50.811 185 84 3 173 352 122 304 3.31e-43 152
MS.gene02239.t1 AT2G22750 50.811 185 84 3 173 352 150 332 6.92e-43 152
MS.gene02239.t1 AT2G22750 50.811 185 84 3 173 352 146 328 7.00e-43 152
MS.gene02239.t1 AT2G22750 50.811 185 83 4 173 352 122 303 6.96e-42 148
MS.gene02239.t1 AT2G22750 50.811 185 83 4 173 352 150 331 1.48e-41 148
MS.gene02239.t1 AT2G22760 47.468 158 81 2 175 330 117 274 1.50e-41 147
MS.gene02239.t1 AT1G32640 36.025 161 87 4 165 313 432 588 1.23e-18 87.8
MS.gene02239.t1 AT5G46760 34.783 161 101 2 177 334 415 574 3.96e-17 83.2
MS.gene02239.t1 AT2G46510 59.649 57 23 0 177 233 395 451 1.22e-16 81.6
MS.gene02239.t1 AT4G16430 37.143 140 70 5 118 241 247 384 3.13e-16 80.1
MS.gene02239.t1 AT1G01260 52.174 69 32 1 168 235 423 491 9.37e-16 79.0
MS.gene02239.t1 AT1G01260 52.174 69 32 1 168 235 423 491 9.37e-16 79.0
MS.gene02239.t1 AT1G01260 52.174 69 32 1 168 235 423 491 9.37e-16 79.0
MS.gene02239.t1 AT4G17880 31.013 158 107 2 177 334 416 571 1.39e-15 78.2
MS.gene02239.t1 AT1G12860 30.921 152 100 3 178 324 268 419 2.12e-14 74.3
MS.gene02239.t1 AT1G12860 31.126 151 99 3 179 324 269 419 2.77e-14 73.9
MS.gene02239.t1 AT3G26744 30.128 156 100 3 178 324 308 463 2.77e-13 71.2
MS.gene02239.t1 AT3G26744 30.128 156 100 3 178 324 308 463 3.31e-13 70.9
MS.gene02239.t1 AT3G26744 30.128 156 100 3 178 324 308 463 3.31e-13 70.9
MS.gene02239.t1 AT3G26744 30.128 156 100 3 178 324 308 463 3.31e-13 70.9
MS.gene02239.t1 AT5G57150 30.000 170 97 5 173 327 51 213 1.08e-12 67.0
MS.gene02239.t1 AT5G57150 30.000 170 97 5 173 327 50 212 1.23e-12 67.0
MS.gene02239.t1 AT5G57150 30.000 170 97 5 173 327 51 213 1.23e-12 67.0
MS.gene02239.t1 AT4G09820 31.343 134 65 3 178 308 364 473 1.63e-12 68.9
MS.gene02239.t1 AT5G57150 30.000 170 97 5 173 327 51 213 1.94e-12 67.0
MS.gene02239.t1 AT5G57150 30.000 170 97 5 173 327 50 212 2.17e-12 66.6
MS.gene02239.t1 AT2G16910 28.049 164 89 4 179 317 316 475 2.23e-12 68.6
MS.gene02239.t1 AT5G41315 28.824 170 99 3 160 307 429 598 2.34e-12 68.6
MS.gene02239.t1 AT5G41315 28.824 170 99 3 160 307 432 601 2.57e-12 68.6
MS.gene02239.t1 AT5G41315 28.824 170 99 3 160 307 424 593 2.57e-12 68.6
MS.gene02239.t1 AT3G61950 30.496 141 87 3 177 311 128 263 4.43e-12 66.6
MS.gene02239.t1 AT4G00870 46.875 64 34 0 178 241 250 313 5.77e-12 67.0
MS.gene02239.t1 AT3G61950 30.496 141 87 3 177 311 179 314 9.13e-12 65.9
MS.gene02239.t1 AT3G61950 30.496 141 87 3 177 311 128 263 9.71e-12 65.5
MS.gene02239.t1 AT3G61950 30.496 141 87 3 177 311 179 314 1.75e-11 65.1
MS.gene02239.t1 AT4G29930 27.778 180 107 6 178 349 55 219 2.01e-11 63.5
MS.gene02239.t1 AT1G63650 26.923 156 97 2 169 307 397 552 2.02e-11 65.5
MS.gene02239.t1 AT1G63650 26.923 156 97 2 169 307 397 552 2.02e-11 65.5
MS.gene02239.t1 AT1G63650 26.923 156 97 2 169 307 397 552 2.02e-11 65.5
MS.gene02239.t1 AT4G29930 28.161 174 111 4 178 349 55 216 2.99e-11 63.2
MS.gene02239.t1 AT4G29930 25.773 194 119 3 178 349 55 245 5.63e-11 62.8

Find 53 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATCTTGCTTCAATTTC+AGG 0.082646 2.1:+73068422 None:intergenic
AATATATATCTTACCATTTC+AGG 0.177016 2.1:+73069950 None:intergenic
AACTTAACTGCTTCTGCTTT+TGG 0.208058 2.1:-73069630 MS.gene02239:CDS
AGCAGAAGCAGTTAAGTTAT+TGG 0.221213 2.1:+73069635 None:intergenic
ATGGACAAAGCTTCTGTTCT+TGG 0.240264 2.1:-73068617 MS.gene02239:CDS
AATAGCAGTTCCTTGACTTT+TGG 0.241912 2.1:-73068302 MS.gene02239:CDS
TTTCACCGGTCTTTCGTTTC+CGG 0.252447 2.1:+73069533 None:intergenic
GTTGTTGAAGTTTGGATTAT+TGG 0.271697 2.1:+73069581 None:intergenic
CATGTTCTGTTGTTGAAGTT+TGG 0.279441 2.1:+73069573 None:intergenic
ATCACTCAAGGTAACTTGTT+TGG 0.298879 2.1:-73069318 MS.gene02239:CDS
TTTGATACCTTCTTTAGGTT+TGG 0.325711 2.1:+73069144 None:intergenic
CATTATTTGATACCTTCTTT+AGG 0.327577 2.1:+73069139 None:intergenic
TCACTCAAGGTAACTTGTTT+GGG 0.334384 2.1:-73069317 MS.gene02239:CDS
TTAAAGTGATGGAGAAAGTT+AGG 0.337641 2.1:+73069681 None:intergenic
CTGTTCTTGGAGAAGCTATA+AGG 0.338206 2.1:-73068604 MS.gene02239:CDS
TATTGGTATCAATAGAGTTT+AGG 0.363520 2.1:+73069652 None:intergenic
TATCAGAGGGTTACCTGAAA+TGG 0.381568 2.1:-73069963 MS.gene02239:intron
TTCCAGGATGAGTTCTCATA+TGG 0.444780 2.1:-73069996 None:intergenic
TAAAATTACCTGAGCAATGA+TGG 0.451657 2.1:+73068259 None:intergenic
TTGAATTACACAAATCAGTT+GGG 0.457187 2.1:-73069411 MS.gene02239:CDS
TCAAACAATGTAGTCTTATG+AGG 0.459967 2.1:+73068387 None:intergenic
TATAAGGTACTTAAAGCAAA+TGG 0.467859 2.1:-73068588 MS.gene02239:CDS
GAGCAATGATGGTTATATCA+AGG 0.480152 2.1:+73068270 None:intergenic
TTAGGTGATTATTAAAGTGA+TGG 0.488258 2.1:+73069670 None:intergenic
TTTGAATTACACAAATCAGT+TGG 0.490344 2.1:-73069412 MS.gene02239:CDS
TTATTTCTTAACTGTTTCAC+CGG 0.499185 2.1:+73069519 None:intergenic
AACAGTTAAGAAATAACAGC+TGG 0.500916 2.1:-73069512 MS.gene02239:CDS
TGATATAACCATCATTGCTC+AGG 0.502657 2.1:-73068267 MS.gene02239:intron
GGACACACCATCTCAGACTT+AGG 0.503073 2.1:+73069378 None:intergenic
AGAGAAAGTGAGTGTTCTTG+AGG 0.506732 2.1:-73068564 MS.gene02239:CDS
CTTGAACAGCTTTAAAGACA+TGG 0.514024 2.1:+73069286 None:intergenic
TCATATTGTGTATCTGATGT+TGG 0.522543 2.1:+73069471 None:intergenic
GGTCTTTCGTTTCCGGTAGA+CGG 0.530949 2.1:+73069540 None:intergenic
TGGCAATTTGCAGATGGATG+TGG 0.531836 2.1:-73067870 MS.gene02239:intron
TGCATGGAGACTCCGTCTAC+CGG 0.552215 2.1:-73069552 MS.gene02239:CDS
TCTTGTTCCAAACCTAAAGA+AGG 0.556354 2.1:-73069151 MS.gene02239:intron
CTACATTGTTTGAAGAGTCA+AGG 0.557167 2.1:-73068377 MS.gene02239:CDS
TGTCTTTAAAGCTGTTCAAG+AGG 0.569037 2.1:-73069283 MS.gene02239:CDS
TGTGTAATTCAAATCAGCAA+AGG 0.572771 2.1:+73069422 None:intergenic
GCAAACATGTTGATCACTCA+AGG 0.574022 2.1:-73069330 MS.gene02239:CDS
AGCAATGATGGTTATATCAA+GGG 0.578216 2.1:+73068271 None:intergenic
CAGACAGAGCGATGAATCGT+TGG 0.578725 2.1:+73069175 None:intergenic
TGGAGATTTCATCTATCAGA+GGG 0.587975 2.1:-73069976 MS.gene02239:CDS
GAAAGTGAGTGTTCTTGAGG+AGG 0.588414 2.1:-73068561 MS.gene02239:CDS
ATTGAAGCCTAAGTCTGAGA+TGG 0.590244 2.1:-73069385 MS.gene02239:CDS
CTTCAACAACAGAACATGCA+TGG 0.601083 2.1:-73069568 MS.gene02239:CDS
ATGGAGATTTCATCTATCAG+AGG 0.606668 2.1:-73069977 MS.gene02239:CDS
GGTGAAATTCCCAAAAGTCA+AGG 0.621239 2.1:+73068292 None:intergenic
TATTGTGTATCTGATGTTGG+AGG 0.636554 2.1:+73069474 None:intergenic
GTCTACCGGAAACGAAAGAC+CGG 0.653717 2.1:-73069538 MS.gene02239:CDS
ATGATCACATCGTAGCGGAG+AGG 0.690331 2.1:-73069218 MS.gene02239:CDS
TGACAGTGAAGGATCTTGTG+AGG 0.718300 2.1:-73067838 MS.gene02239:CDS
AGCTCATGATCACATCGTAG+CGG 0.723921 2.1:-73069223 MS.gene02239:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTAGTTAATTATCA+GGG - chr2.1:73068868-73068887 MS.gene02239:intron 10.0%
!! TCCAATATTCATATAAAATT+AGG + chr2.1:73069898-73069917 None:intergenic 15.0%
!! TTAACATTAGATATACATAT+AGG - chr2.1:73069127-73069146 MS.gene02239:intron 15.0%
!!! AAAAGTATATATTTCATCTT+AGG - chr2.1:73068707-73068726 MS.gene02239:intron 15.0%
!!! CTTTTTTAATAGTTTATCTT+AGG + chr2.1:73068993-73069012 None:intergenic 15.0%
!!! GTAATATTTCAATTTTGATT+AGG - chr2.1:73068771-73068790 MS.gene02239:intron 15.0%
!!! GTTATTTTTAGTTAATTATC+AGG - chr2.1:73068867-73068886 MS.gene02239:intron 15.0%
!!! TCCTAATTTTATATGAATAT+TGG - chr2.1:73069894-73069913 MS.gene02239:intron 15.0%
!! AAATGGTTCAAAAATAAAGT+AGG + chr2.1:73067962-73067981 None:intergenic 20.0%
!! AATATATATCTTACCATTTC+AGG + chr2.1:73067847-73067866 None:intergenic 20.0%
!! AATTGTGTATCAAGTATTAT+TGG - chr2.1:73068845-73068864 MS.gene02239:intron 20.0%
!! ATATGAATATTGGAATTGTT+TGG - chr2.1:73069904-73069923 MS.gene02239:intron 20.0%
!! ATTCATATAAAATTAGGACT+TGG + chr2.1:73069892-73069911 None:intergenic 20.0%
!! TTGATTCAAAATATGGTATT+TGG - chr2.1:73067990-73068009 MS.gene02239:intron 20.0%
!!! AGTCTAAATTGATTCAATTT+TGG + chr2.1:73067893-73067912 None:intergenic 20.0%
!!! TATATACTTTTGACAATATG+AGG + chr2.1:73068698-73068717 None:intergenic 20.0%
! CATTATTTGATACCTTCTTT+AGG + chr2.1:73068658-73068677 None:intergenic 25.0%
! TATTGGTATCAATAGAGTTT+AGG + chr2.1:73068145-73068164 None:intergenic 25.0%
! TTAGGTGATTATTAAAGTGA+TGG + chr2.1:73068127-73068146 None:intergenic 25.0%
! TTGAATCAACATGTGTTAAA+TGG + chr2.1:73067979-73067998 None:intergenic 25.0%
! TTGAATTACACAAATCAGTT+GGG - chr2.1:73068383-73068402 MS.gene02239:CDS 25.0%
! TTTGAATTACACAAATCAGT+TGG - chr2.1:73068382-73068401 MS.gene02239:CDS 25.0%
!! AATGTTTTATGTTTTCTGCA+GGG - chr2.1:73068077-73068096 MS.gene02239:intron 25.0%
!! TAATGTTTTATGTTTTCTGC+AGG - chr2.1:73068076-73068095 MS.gene02239:intron 25.0%
!! TATAAGGTACTTAAAGCAAA+TGG - chr2.1:73069206-73069225 MS.gene02239:CDS 25.0%
!! TTATTTCTTAACTGTTTCAC+CGG + chr2.1:73068278-73068297 None:intergenic 25.0%
!! TTCAGTTTTCTGTATATAGA+TGG - chr2.1:73069158-73069177 MS.gene02239:CDS 25.0%
!! TTTTTGTAAACACCTATTCT+TGG - chr2.1:73067866-73067885 MS.gene02239:intron 25.0%
!!! ACACATGTTGATTCAAAATA+TGG - chr2.1:73067983-73068002 MS.gene02239:intron 25.0%
!!! CTAAAGAAATTTTAACCAAG+AGG + chr2.1:73068907-73068926 None:intergenic 25.0%
!!! GATTGATTGTTATTTGTTGA+TGG + chr2.1:73069777-73069796 None:intergenic 25.0%
AACAGTTAAGAAATAACAGC+TGG - chr2.1:73068282-73068301 MS.gene02239:CDS 30.0%
AACATCGTGATATCTTTGTT+AGG - chr2.1:73068749-73068768 MS.gene02239:intron 30.0%
ACAAAAAAGGAAGAAAACTG+TGG - chr2.1:73069257-73069276 MS.gene02239:CDS 30.0%
AGCAATGATGGTTATATCAA+GGG + chr2.1:73069526-73069545 None:intergenic 30.0%
CAAAATCTTGCTTCAATTTC+AGG + chr2.1:73069375-73069394 None:intergenic 30.0%
GTTGTTGAAGTTTGGATTAT+TGG + chr2.1:73068216-73068235 None:intergenic 30.0%
TAAAATTACCTGAGCAATGA+TGG + chr2.1:73069538-73069557 None:intergenic 30.0%
TCATATTGTGTATCTGATGT+TGG + chr2.1:73068326-73068345 None:intergenic 30.0%
TGTGTAATTCAAATCAGCAA+AGG + chr2.1:73068375-73068394 None:intergenic 30.0%
! ATGTTTTATAGTTCCTCGTA+AGG + chr2.1:73068734-73068753 None:intergenic 30.0%
! ATGTTTTATGTTTTCTGCAG+GGG - chr2.1:73068078-73068097 MS.gene02239:intron 30.0%
! TGAGCTTGAGAGATTTTATT+AGG + chr2.1:73068557-73068576 None:intergenic 30.0%
! TTAAAGTGATGGAGAAAGTT+AGG + chr2.1:73068116-73068135 None:intergenic 30.0%
! TTTGATACCTTCTTTAGGTT+TGG + chr2.1:73068653-73068672 None:intergenic 30.0%
! TTTTACATACTCATGATTCC+TGG + chr2.1:73068827-73068846 None:intergenic 30.0%
!! TCAAACAATGTAGTCTTATG+AGG + chr2.1:73069410-73069429 None:intergenic 30.0%
!! TTCAATTTTGGTCCAAGAAT+AGG + chr2.1:73067881-73067900 None:intergenic 30.0%
!!! ATCTAGCTTCTGAAATTTTG+TGG - chr2.1:73068031-73068050 MS.gene02239:intron 30.0%
!!! CATGTTTTTTATGACAAACC+AGG - chr2.1:73068806-73068825 MS.gene02239:intron 30.0%
!!! GTACTAGTGTTATCGATTTT+CGG + chr2.1:73068440-73068459 None:intergenic 30.0%
AGCAGAAGCAGTTAAGTTAT+TGG + chr2.1:73068162-73068181 None:intergenic 35.0%
ATGGAGATTTCATCTATCAG+AGG - chr2.1:73067817-73067836 MS.gene02239:CDS 35.0%
ATTGTTTGGCAATTTGCAGA+TGG - chr2.1:73069918-73069937 MS.gene02239:intron 35.0%
CATGTTCTGTTGTTGAAGTT+TGG + chr2.1:73068224-73068243 None:intergenic 35.0%
CTACATTGTTTGAAGAGTCA+AGG - chr2.1:73069417-73069436 MS.gene02239:CDS 35.0%
GAGCAATGATGGTTATATCA+AGG + chr2.1:73069527-73069546 None:intergenic 35.0%
TATTGTGTATCTGATGTTGG+AGG + chr2.1:73068323-73068342 None:intergenic 35.0%
TCTTGTTCCAAACCTAAAGA+AGG - chr2.1:73068643-73068662 MS.gene02239:intron 35.0%
TGATATAACCATCATTGCTC+AGG - chr2.1:73069527-73069546 MS.gene02239:CDS 35.0%
TGGAGATTTCATCTATCAGA+GGG - chr2.1:73067818-73067837 MS.gene02239:CDS 35.0%
TTCTTGAGGAGGAACAAAAA+AGG - chr2.1:73069244-73069263 MS.gene02239:CDS 35.0%
! AACTTAACTGCTTCTGCTTT+TGG - chr2.1:73068164-73068183 MS.gene02239:intron 35.0%
! AATAGCAGTTCCTTGACTTT+TGG - chr2.1:73069492-73069511 MS.gene02239:CDS 35.0%
! ATAGCAGTTCCTTGACTTTT+GGG - chr2.1:73069493-73069512 MS.gene02239:CDS 35.0%
!! ATCACTCAAGGTAACTTGTT+TGG - chr2.1:73068476-73068495 MS.gene02239:CDS 35.0%
!! CTTGAACAGCTTTAAAGACA+TGG + chr2.1:73068511-73068530 None:intergenic 35.0%
!! TCACTCAAGGTAACTTGTTT+GGG - chr2.1:73068477-73068496 MS.gene02239:CDS 35.0%
!! TCTGAAATTTTGTGGTAGCT+AGG - chr2.1:73068039-73068058 MS.gene02239:intron 35.0%
!! TGTCTTTAAAGCTGTTCAAG+AGG - chr2.1:73068511-73068530 MS.gene02239:CDS 35.0%
AGAGAAAGTGAGTGTTCTTG+AGG - chr2.1:73069230-73069249 MS.gene02239:CDS 40.0%
ATACCAACTAACTCGGATAG+AGG - chr2.1:73068938-73068957 MS.gene02239:intron 40.0%
ATGGACAAAGCTTCTGTTCT+TGG - chr2.1:73069177-73069196 MS.gene02239:CDS 40.0%
ATTGAAGCCTAAGTCTGAGA+TGG - chr2.1:73068409-73068428 MS.gene02239:CDS 40.0%
CAAATGTCATCTTTCCAAGC+AGG + chr2.1:73069076-73069095 None:intergenic 40.0%
CCTCGATATACCAACTAACT+CGG - chr2.1:73068931-73068950 MS.gene02239:intron 40.0%
CTGTTCTTGGAGAAGCTATA+AGG - chr2.1:73069190-73069209 MS.gene02239:CDS 40.0%
CTTCAACAACAGAACATGCA+TGG - chr2.1:73068226-73068245 MS.gene02239:intron 40.0%
GCAAACATGTTGATCACTCA+AGG - chr2.1:73068464-73068483 MS.gene02239:CDS 40.0%
GGTGAAATTCCCAAAAGTCA+AGG + chr2.1:73069505-73069524 None:intergenic 40.0%
TATCAGAGGGTTACCTGAAA+TGG - chr2.1:73067831-73067850 MS.gene02239:CDS 40.0%
TTTCATCTTAGGTCCTTACG+AGG - chr2.1:73068718-73068737 MS.gene02239:intron 40.0%
TTTCCTCTATCCGAGTTAGT+TGG + chr2.1:73068944-73068963 None:intergenic 40.0%
! ATGTTTTCTGCAGGGGATAA+TGG - chr2.1:73068085-73068104 MS.gene02239:intron 40.0%
! CGAGTTAGTTGGTATATCGA+GGG + chr2.1:73068933-73068952 None:intergenic 40.0%
! GGAATTTTGCATGACAGTGA+AGG - chr2.1:73069945-73069964 MS.gene02239:intron 40.0%
AGCTCATGATCACATCGTAG+CGG - chr2.1:73068571-73068590 MS.gene02239:CDS 45.0%
GAAAGTGAGTGTTCTTGAGG+AGG - chr2.1:73069233-73069252 MS.gene02239:CDS 45.0%
GGTTTGAACTCTAGACCTCT+TGG - chr2.1:73068889-73068908 MS.gene02239:intron 45.0%
TGACAGTGAAGGATCTTGTG+AGG - chr2.1:73069956-73069975 MS.gene02239:intron 45.0%
TGGCAATTTGCAGATGGATG+TGG - chr2.1:73069924-73069943 MS.gene02239:intron 45.0%
TTTCACCGGTCTTTCGTTTC+CGG + chr2.1:73068264-73068283 None:intergenic 45.0%
! CCGAGTTAGTTGGTATATCG+AGG + chr2.1:73068934-73068953 None:intergenic 45.0%
!! AGGTAGCTCAGTTGGTAAGT+TGG + chr2.1:73069056-73069075 None:intergenic 45.0%
!! GGTAGCTCAGTTGGTAAGTT+GGG + chr2.1:73069055-73069074 None:intergenic 45.0%
!! GTAGCTCAGTTGGTAAGTTG+GGG + chr2.1:73069054-73069073 None:intergenic 45.0%
!! TTTTCTGCAGGGGATAATGG+AGG - chr2.1:73068088-73068107 MS.gene02239:intron 45.0%
CAGACAGAGCGATGAATCGT+TGG + chr2.1:73068622-73068641 None:intergenic 50.0%
GGACACACCATCTCAGACTT+AGG + chr2.1:73068419-73068438 None:intergenic 50.0%
GGTCTTTCGTTTCCGGTAGA+CGG + chr2.1:73068257-73068276 None:intergenic 50.0%
GTCTACCGGAAACGAAAGAC+CGG - chr2.1:73068256-73068275 MS.gene02239:intron 50.0%
TACCAACTGAGCTACCTGCT+TGG - chr2.1:73069059-73069078 MS.gene02239:intron 50.0%
TTCCAAGCAGGTAGCTCAGT+TGG + chr2.1:73069064-73069083 None:intergenic 50.0%
! ATGATCACATCGTAGCGGAG+AGG - chr2.1:73068576-73068595 MS.gene02239:CDS 50.0%
! GTAAGTTGGGGTCAGCTACA+TGG + chr2.1:73069042-73069061 None:intergenic 50.0%
TGCATGGAGACTCCGTCTAC+CGG - chr2.1:73068242-73068261 MS.gene02239:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 73067805 73070011 73067805 ID=MS.gene02239
chr2.1 mRNA 73067805 73070011 73067805 ID=MS.gene02239.t1;Parent=MS.gene02239
chr2.1 exon 73069964 73070011 73069964 ID=MS.gene02239.t1.exon1;Parent=MS.gene02239.t1
chr2.1 CDS 73069964 73070011 73069964 ID=cds.MS.gene02239.t1;Parent=MS.gene02239.t1
chr2.1 exon 73069152 73069718 73069152 ID=MS.gene02239.t1.exon2;Parent=MS.gene02239.t1
chr2.1 CDS 73069152 73069718 73069152 ID=cds.MS.gene02239.t1;Parent=MS.gene02239.t1
chr2.1 exon 73068268 73068639 73068268 ID=MS.gene02239.t1.exon3;Parent=MS.gene02239.t1
chr2.1 CDS 73068268 73068639 73068268 ID=cds.MS.gene02239.t1;Parent=MS.gene02239.t1
chr2.1 exon 73067805 73067879 73067805 ID=MS.gene02239.t1.exon4;Parent=MS.gene02239.t1
chr2.1 CDS 73067805 73067879 73067805 ID=cds.MS.gene02239.t1;Parent=MS.gene02239.t1
Gene Sequence

>MS.gene02239

ATGAGTTCTCATATGGAGATTTCATCTATCAGAGGGTTACCTGAAATGGTAAGATATATATTTTTTGTAAACACCTATTCTTGGACCAAAATTGAATCAATTTAGACTATTCAAGTTAATAGTGTGAATCATTTATTATATATGTTTACATTGACCTACTTTATTTTTGAACCATTTAACACATGTTGATTCAAAATATGGTATTTGGTTGATGAAATTTCTATGAATCTAGCTTCTGAAATTTTGTGGTAGCTAGGACTAATTAAGAGTATAATGTTTTATGTTTTCTGCAGGGGATAATGGAGGATCCTAACTTTCTCCATCACTTTAATAATCACCTAAACTCTATTGATACCAATAACTTAACTGCTTCTGCTTTTGGAGATGCTTTACAAAAACATATTTTGTCCAATAATCCAAACTTCAACAACAGAACATGCATGGAGACTCCGTCTACCGGAAACGAAAGACCGGTGAAACAGTTAAGAAATAACAGCTGGAATTACAACAATAGTCCTCCAACATCAGATACACAATATGATAATTGTTGCTCTAATAATCTTCTTTCCTTTGCTGATTTGAATTACACAAATCAGTTGGGACTATTGAAGCCTAAGTCTGAGATGGTGTGTCCGAAAATCGATAACACTAGTACTCTTGCAAACATGTTGATCACTCAAGGTAACTTGTTTGGGAATCAAAACCATGTCTTTAAAGCTGTTCAAGAGGCTAAAGATATTGAGAATCGTCCTAATAAAATCTCTCAAGCTCATGATCACATCGTAGCGGAGAGGAAGCGTCGCGAGAAGCTTAGCCAACGATTCATCGCTCTGTCTGCTCTTGTTCCAAACCTAAAGAAGGTATCAAATAATGTTGTTAATGTATTATTGCCTCATATTGTCAAAAGTATATATTTCATCTTAGGTCCTTACGAGGAACTATAAAACATCGTGATATCTTTGTTAGGTAATATTTCAATTTTGATTAGGATAAATATTAAACATGTTTTTTATGACAAACCAGGAATCATGAGTATGTAAAATTGTGTATCAAGTATTATTGGTTATTTTTAGTTAATTATCAGGGTTTGAACTCTAGACCTCTTGGTTAAAATTTCTTTAGTGTCCCTCGATATACCAACTAACTCGGATAGAGGAAAGTCAGATCACTAGAGACAGATGAAGACCTAAGATAAACTATTAAAAAAGATTTAGAGATTATAGCGTAATTTGATCCATGTAGCTGACCCCAACTTACCAACTGAGCTACCTGCTTGGAAAGATGACATTTGTGTTCAAATAATGCAGTGTCAATTTTTAAACTTAACATTAGATATACATATAGGTCTCATTTTTCAGTTTTCTGTATATAGATGGACAAAGCTTCTGTTCTTGGAGAAGCTATAAGGTACTTAAAGCAAATGGAAGAGAAAGTGAGTGTTCTTGAGGAGGAACAAAAAAGGAAGAAAACTGTGGAATCTGTTGTAATAGTGAAGAAATCTCAACTTTCTATTAATGATGCTGAAAACTCTTGTTCAGACACTGATAGTACATTTGATGAGACATTACCTGAAATTGAAGCAAGATTTTGTGAAAGAAGTGTCCTCATAAGACTACATTGTTTGAAGAGTCAAGGAGTTATTGAGAAAATTATGAGTGAAATTGAGAAACTTCATCTGAAAGTCATCAATAGCAGTTCCTTGACTTTTGGGAATTTCACCCTTGATATAACCATCATTGCTCAGGTAATTTTACGAGTAATTAATTATATTACTCTAAGCATTATGATTAATTAGTTCACACAATATAGATATTGTTTTAAGTTACATTATTTATAAAAGTAACACAAAAATGTTGATTATACAAATTAAATTAAATACTCAGTACTCACACTCAGACACATAATATGTATTGTACATGCAACATTCTATTTTTTCAAAAAATTAATTTTAATTTTAAAAAGTTTTACACCATCAACAAATAACAATCAATCATATATGCGAATTTAAAAGCAGTTATAATGAAAAGTAAATATTTTTAATGATCTCGCAATTATGATTGATTGATCATGTAAATAAAATTACGCCAAGTCCTAATTTTATATGAATATTGGAATTGTTTGGCAATTTGCAGATGGATGTGGAATTTTGCATGACAGTGAAGGATCTTGTGAGGAAGCTACGTTCAGCATATTCATCTTTCATGTGA
Protein sequence

>MS.gene02239.t1

MSSHMEISSIRGLPEMGIMEDPNFLHHFNNHLNSIDTNNLTASAFGDALQKHILSNNPNFNNRTCMETPSTGNERPVKQLRNNSWNYNNSPPTSDTQYDNCCSNNLLSFADLNYTNQLGLLKPKSEMVCPKIDNTSTLANMLITQGNLFGNQNHVFKAVQEAKDIENRPNKISQAHDHIVAERKRREKLSQRFIALSALVPNLKKMDKASVLGEAIRYLKQMEEKVSVLEEEQKRKKTVESVVIVKKSQLSINDAENSCSDTDSTFDETLPEIEARFCERSVLIRLHCLKSQGVIEKIMSEIEKLHLKVINSSSLTFGNFTLDITIIAQMDVEFCMTVKDLVRKLRSAYSSFM