AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022461

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022461.t1 MTR_3g074930 92.370 616 46 1 4 619 8 622 0.0 1210
MS.gene022461.t1 MTR_3g074930 91.332 623 46 2 4 619 8 629 0.0 1204
MS.gene022461.t1 MTR_4g103520 77.600 625 130 3 1 619 6 626 0.0 1040
MS.gene022461.t1 MTR_4g103520 81.193 553 98 2 69 619 1 549 0.0 964
MS.gene022461.t1 MTR_8g063080 68.943 615 184 2 5 619 4 611 0.0 909
MS.gene022461.t1 MTR_5g045600 70.230 608 176 2 14 619 14 618 0.0 908
MS.gene022461.t1 MTR_3g074970 84.713 471 27 2 148 618 100 525 0.0 822
MS.gene022461.t1 MTR_3g074970 95.833 96 4 0 517 612 3 98 1.89e-54 194
MS.gene022461.t1 MTR_3g074960 72.154 492 72 5 4 474 8 455 0.0 706
MS.gene022461.t1 MTR_4g037670 35.640 578 312 18 61 600 79 634 7.76e-107 336
MS.gene022461.t1 MTR_1g052505 35.000 560 309 16 78 600 74 615 4.31e-103 325
MS.gene022461.t1 MTR_4g082930 28.454 608 352 23 42 598 5 580 3.72e-58 207
MS.gene022461.t1 MTR_4g082940 27.632 608 346 23 40 598 4 566 8.88e-52 189
MS.gene022461.t1 MTR_6g005650 82.051 78 14 0 189 266 10 87 2.24e-37 141
MS.gene022461.t1 MTR_1g060360 27.229 415 228 15 189 593 57 407 1.16e-29 122
MS.gene022461.t1 MTR_7g104330 25.908 413 228 15 189 589 65 411 4.89e-29 120
MS.gene022461.t1 MTR_4g115670 26.190 462 277 16 160 602 35 451 9.80e-28 117
MS.gene022461.t1 MTR_7g104360 25.791 411 232 13 189 589 58 405 1.70e-27 115
MS.gene022461.t1 MTR_3g051240 26.218 431 246 14 189 613 54 418 2.83e-26 112
MS.gene022461.t1 MTR_6g013050 27.053 414 186 15 246 589 146 513 1.64e-25 111
MS.gene022461.t1 MTR_6g013050 27.204 397 173 15 246 572 146 496 7.47e-23 103
MS.gene022461.t1 MTR_5g092360 24.390 451 263 18 173 613 42 424 1.46e-22 101
MS.gene022461.t1 MTR_7g056403 25.352 426 202 13 246 600 146 526 2.55e-22 101
MS.gene022461.t1 MTR_3g083810 24.186 430 202 14 246 600 147 527 7.71e-22 100
MS.gene022461.t1 MTR_3g083820 23.776 429 205 14 246 600 147 527 1.68e-20 95.9
MS.gene022461.t1 MTR_0188s0020 24.796 367 223 13 246 595 119 449 4.05e-20 94.4
MS.gene022461.t1 MTR_1g112630 24.122 427 242 19 190 590 70 440 3.53e-18 88.2
MS.gene022461.t1 MTR_4g037105 24.793 363 218 15 246 590 113 438 5.87e-18 87.4
MS.gene022461.t1 MTR_7g405800 93.333 30 2 0 232 261 1 30 6.49e-14 72.0
MS.gene022461.t1 MTR_2g020270 68.085 47 13 1 119 163 33 79 1.01e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022461.t1 AT5G50400 73.159 611 158 3 9 619 6 610 0.0 947
MS.gene022461.t1 AT4G24890 72.287 599 163 2 21 619 19 614 0.0 924
MS.gene022461.t1 AT1G13750 66.667 615 202 1 5 619 1 612 0.0 876
MS.gene022461.t1 AT5G50400 78.824 510 107 1 110 619 30 538 0.0 863
MS.gene022461.t1 AT1G13900 29.568 602 323 26 50 598 24 577 1.37e-58 208
MS.gene022461.t1 AT2G03450 28.642 611 333 25 43 598 15 577 2.16e-57 205
MS.gene022461.t1 AT3G52780 26.218 431 244 15 172 589 36 405 1.85e-28 119
MS.gene022461.t1 AT3G52810 26.450 431 243 17 169 589 42 408 1.39e-27 116
MS.gene022461.t1 AT3G52820 26.728 434 248 18 162 589 35 404 4.12e-24 105
MS.gene022461.t1 AT4G13700 26.970 330 171 11 246 547 140 427 6.59e-24 105
MS.gene022461.t1 AT3G20500 30.466 279 163 10 320 589 147 403 6.67e-24 105
MS.gene022461.t1 AT2G27190 27.671 365 205 15 246 590 118 443 9.42e-23 102
MS.gene022461.t1 AT3G52780 27.507 349 198 12 172 513 36 336 1.52e-22 100
MS.gene022461.t1 AT5G34850 25.203 369 234 12 246 602 112 450 2.02e-22 101
MS.gene022461.t1 AT4G36350 24.044 366 218 13 246 591 114 439 1.35e-19 92.4
MS.gene022461.t1 AT3G07130 24.466 421 209 15 246 600 140 517 2.48e-19 92.0
MS.gene022461.t1 AT4G13700 26.804 291 153 10 246 508 140 398 5.69e-19 90.1
MS.gene022461.t1 AT1G56360 22.782 417 264 16 191 591 65 439 1.01e-18 89.7
MS.gene022461.t1 AT2G18130 25.714 280 179 10 338 602 168 433 4.45e-15 78.6
MS.gene022461.t1 AT2G32770 22.170 424 201 16 246 589 149 523 6.92e-15 78.2
MS.gene022461.t1 AT3G07130 22.676 441 225 16 246 600 140 550 1.07e-14 77.8
MS.gene022461.t1 AT2G32770 23.343 347 172 14 246 547 68 365 8.28e-13 71.2

Find 167 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTTCATTTACCTTTAT+TGG 0.162930 3.2:-67422198 None:intergenic
TTTAATGCACACCTCATATT+TGG 0.186772 3.2:-67423362 None:intergenic
TAGTAGTTATACCTGATAAA+TGG 0.220072 3.2:-67425152 None:intergenic
GAGTTTCATTTACCTTTATT+GGG 0.220701 3.2:-67422197 None:intergenic
CAGTCTTCATTAGTTGGTTC+TGG 0.230728 3.2:-67420754 None:intergenic
TACCTTACTGCCAAGCAAAA+AGG 0.234836 3.2:-67418698 None:intergenic
TAATATAACTCACCAGAATT+TGG 0.238636 3.2:-67424162 None:intergenic
TGAATCTGCTTTCCACCCTT+TGG 0.258361 3.2:-67423127 None:intergenic
TGCAGGTATGCCACAGATTA+TGG 0.265751 3.2:+67424848 MS.gene022461:intron
GAATCTGCTTTCCACCCTTT+GGG 0.297542 3.2:-67423126 None:intergenic
GCGAACCTTGCTATCATATA+TGG 0.299475 3.2:-67423932 None:intergenic
GGGAAAGTTTGCAGGGGCTT+TGG 0.317516 3.2:+67425068 MS.gene022461:CDS
AGAGACAAAGACTATGGTTT+TGG 0.320936 3.2:+67425416 MS.gene022461:CDS
CATGGGAAGGGAAAGTTTGC+AGG 0.323081 3.2:+67425060 MS.gene022461:CDS
TTCTATGACACTCCAGATTC+AGG 0.324023 3.2:+67424083 MS.gene022461:CDS
CGCGGATACACAGGTGCTTT+AGG 0.325055 3.2:-67422853 None:intergenic
CTGCACGTTGATTGATCAAT+TGG 0.331585 3.2:-67422374 None:intergenic
CCGCATTCTCCACCTGAATC+TGG 0.338489 3.2:-67424095 None:intergenic
TTGGGTGATATGAATGGATT+TGG 0.345669 3.2:+67418598 MS.gene022461:CDS
AAGAGTATGCCCCATTTATC+AGG 0.358857 3.2:+67425141 MS.gene022461:CDS
TTGCAACGTGTTATCATATT+CGG 0.359394 3.2:+67423562 MS.gene022461:CDS
CCCAGGTACGCCTATAGGTT+GGG 0.360573 3.2:+67423460 MS.gene022461:intron
CACCAACTTGAGGATCTGTT+TGG 0.360863 3.2:+67418579 MS.gene022461:CDS
AGTAGTTATACCTGATAAAT+GGG 0.363474 3.2:-67425151 None:intergenic
AGTCTTCATTAGTTGGTTCT+GGG 0.366107 3.2:-67420753 None:intergenic
CGTCCAATGCCACCGGGGTT+GGG 0.366293 3.2:-67422147 None:intergenic
ACCGTTGGCTGGCGCGATCC+TGG 0.370934 3.2:+67423305 MS.gene022461:CDS
GGCATACTCTTTCGTAGTTA+TGG 0.374674 3.2:-67425129 None:intergenic
CTGCAAACTTTCCCTTCCCA+TGG 0.377219 3.2:-67425059 None:intergenic
CATTATTCTGGCACTGTGAA+TGG 0.377604 3.2:+67425314 MS.gene022461:CDS
TCACCACAGCACCTCCTGTT+TGG 0.380045 3.2:+67425384 MS.gene022461:CDS
AGCTGTACCATTTGTTGAAT+GGG 0.384608 3.2:+67423102 MS.gene022461:CDS
GTATGTTACTTGAAGTTTGC+AGG 0.390132 3.2:-67420793 None:intergenic
CAGGTTTCCTTGAAGAACTT+TGG 0.392296 3.2:+67423339 MS.gene022461:CDS
CCGTCCAATGCCACCGGGGT+TGG 0.396303 3.2:-67422148 None:intergenic
AGGTAAGTTAAACATGAGTT+TGG 0.397660 3.2:+67418537 MS.gene022461:CDS
CTGAGAACAAAGCCAAATTC+TGG 0.397715 3.2:+67424150 MS.gene022461:CDS
AGAATGGGTGACAGTAAAAC+TGG 0.399637 3.2:+67420726 MS.gene022461:CDS
CATGTTGTTGTTGGTGGAGG+GGG 0.399824 3.2:+67425344 MS.gene022461:CDS
TGTCTGATGGCTCTTATGTA+TGG 0.401100 3.2:+67423491 MS.gene022461:CDS
CATATGCTCAGCCCCAATAA+AGG 0.402084 3.2:+67422185 MS.gene022461:CDS
TCATAGAAGGATCCTGAGTT+TGG 0.403373 3.2:-67424068 None:intergenic
CAATAAATGCCCCAACCTAT+AGG 0.409594 3.2:-67423470 None:intergenic
TCTTCCCCAGGTACGCCTAT+AGG 0.412213 3.2:+67423455 MS.gene022461:intron
CCAACCCAGTCTTCATTAGT+TGG 0.419485 3.2:-67420760 None:intergenic
ATCTCTATAGTCCCTGGAAA+TGG 0.436173 3.2:-67425519 None:intergenic
TTCAAGGCATCTCCTTATCC+TGG 0.438321 3.2:+67423529 MS.gene022461:CDS
AAGAACTTTGGCCAAATATG+AGG 0.438770 3.2:+67423351 MS.gene022461:CDS
AATTTGGCTTTGTTCTCAGC+AGG 0.439009 3.2:-67424146 None:intergenic
CTTGGCATACCAATCATTAG+AGG 0.440011 3.2:-67425017 None:intergenic
CCACCCAACCCCGGTGGCAT+TGG 0.440027 3.2:+67422144 MS.gene022461:CDS
AACCTGCGCGAACCGTTGGC+TGG 0.449374 3.2:+67423294 MS.gene022461:CDS
TGAATCTGGAGTGTCATAGA+AGG 0.450964 3.2:-67424081 None:intergenic
CTCTTCAAATGAACCCTCCT+TGG 0.453099 3.2:-67425035 None:intergenic
AAGCAGATTCAATCTGCTGC+TGG 0.454182 3.2:+67423139 MS.gene022461:CDS
TTTGGGCCCCATTCAACAAA+TGG 0.461006 3.2:-67423109 None:intergenic
ACCAACTTGAGGATCTGTTT+GGG 0.461280 3.2:+67418580 MS.gene022461:CDS
TATTTGGCCAAAGTTCTTCA+AGG 0.461537 3.2:-67423346 None:intergenic
GGAACAATTCATGTTGTTGT+TGG 0.462326 3.2:+67425335 MS.gene022461:CDS
GTGGAAATCACGAACGCTCT+TGG 0.464456 3.2:+67424045 MS.gene022461:intron
CTCAGCAGGGTAATAGTACA+TGG 0.467918 3.2:-67424132 None:intergenic
ATCTGTTTGGGTGATATGAA+TGG 0.471658 3.2:+67418592 MS.gene022461:CDS
ATTCATGTTGTTGTTGGTGG+AGG 0.474320 3.2:+67425341 MS.gene022461:CDS
TTGGGGCATTTATTGTCTGA+TGG 0.475270 3.2:+67423478 MS.gene022461:CDS
CCCCAGGTACGCCTATAGGT+TGG 0.480019 3.2:+67423459 MS.gene022461:intron
TCTCTGAAAATACTCCAAAC+AGG 0.481381 3.2:-67425398 None:intergenic
CAGGGACTATAGAGATGTCT+TGG 0.482107 3.2:+67425526 MS.gene022461:CDS
GTATTTAGGATTGGAGTGGT+TGG 0.482977 3.2:-67422322 None:intergenic
GTGTATCCGCGCCTTGCTCA+TGG 0.485506 3.2:+67422865 MS.gene022461:CDS
AAGCTGTACCATTTGTTGAA+TGG 0.486572 3.2:+67423101 MS.gene022461:CDS
TTCATGTTGTTGTTGGTGGA+GGG 0.486891 3.2:+67425342 MS.gene022461:CDS
TTTGAAACTGCAACAAGCTT+AGG 0.488913 3.2:-67422811 None:intergenic
TCGGTTACTCCTCTAATGAT+TGG 0.490311 3.2:+67425008 MS.gene022461:CDS
TGTCTAAATCCTTGATGAGC+TGG 0.492936 3.2:-67423805 None:intergenic
ATCTACCCGTCCAATGCCAC+CGG 0.493173 3.2:-67422154 None:intergenic
GCTCTTATGTATGGAGTAAG+AGG 0.494084 3.2:+67423500 MS.gene022461:CDS
ACAATTCATGTTGTTGTTGG+TGG 0.495571 3.2:+67425338 MS.gene022461:CDS
CAGAACCAACTAATGAAGAC+TGG 0.498767 3.2:+67420755 MS.gene022461:CDS
CGCCAGCCAACGGTTCGCGC+AGG 0.499949 3.2:-67423296 None:intergenic
TGCAAACTTTCCCTTCCCAT+GGG 0.500947 3.2:-67425058 None:intergenic
ATTGGGGCTGAGCATATGAA+TGG 0.502132 3.2:-67422180 None:intergenic
AGAATCATGGATCGCAAGAA+TGG 0.504874 3.2:-67418656 None:intergenic
GGAGATCTGCCATATGCAAA+TGG 0.505321 3.2:+67423851 MS.gene022461:CDS
TTTGTTGAATGGGGCCCAAA+GGG 0.507205 3.2:+67423112 MS.gene022461:CDS
AGTTTCATTTACCTTTATTG+GGG 0.515764 3.2:-67422196 None:intergenic
CCAACCCCGGTGGCATTGGA+CGG 0.516793 3.2:+67422148 MS.gene022461:CDS
GAGTAAGAGGTATTCATTCA+AGG 0.520098 3.2:+67423513 MS.gene022461:CDS
GGTCAACCTGCGCGAACCGT+TGG 0.521129 3.2:+67423290 MS.gene022461:intron
CAACCCCGGTGGCATTGGAC+GGG 0.532592 3.2:+67422149 MS.gene022461:CDS
TGAGCAAGGCGCGGATACAC+AGG 0.538957 3.2:-67422862 None:intergenic
GAACGCTCTTGGCCAAACTC+AGG 0.539098 3.2:+67424056 MS.gene022461:CDS
CAGTAACGGAGGCAGAATCA+TGG 0.541508 3.2:-67418669 None:intergenic
TAATAGTACATGGTTTCTGC+AGG 0.541874 3.2:-67424122 None:intergenic
CAAGAAAAGTAGTGATGGAA+AGG 0.542299 3.2:+67425481 MS.gene022461:CDS
GTCCTTTAGGCTGAGCGTGA+TGG 0.547365 3.2:+67423734 MS.gene022461:intron
GTCTTCATTAGTTGGTTCTG+GGG 0.549003 3.2:-67420752 None:intergenic
TCCAAGACTTTCCATGAGCA+AGG 0.549311 3.2:-67422876 None:intergenic
TCATGTTGTTGTTGGTGGAG+GGG 0.552640 3.2:+67425343 MS.gene022461:CDS
TATGACAGATGACAGTTACC+TGG 0.552868 3.2:+67423056 MS.gene022461:intron
TGTTATCATATTCGGTGACA+TGG 0.552892 3.2:+67423570 MS.gene022461:CDS
CCAATGCCACCGGGGTTGGG+TGG 0.553233 3.2:-67422144 None:intergenic
GGGTTCATTTGAAGAGCCCA+TGG 0.554005 3.2:+67425042 MS.gene022461:CDS
CTCTAATGATTGGTATGCCA+AGG 0.554950 3.2:+67425018 MS.gene022461:CDS
TCTACCCGTCCAATGCCACC+GGG 0.555257 3.2:-67422153 None:intergenic
CCAGGTACGCCTATAGGTTG+GGG 0.555647 3.2:+67423461 MS.gene022461:intron
ATTTGTTGAATGGGGCCCAA+AGG 0.559998 3.2:+67423111 MS.gene022461:CDS
TAGCAGACACTGAACATGAC+TGG 0.560714 3.2:+67424888 MS.gene022461:CDS
GCAGGGCGAGGATACAGAAT+GGG 0.561327 3.2:+67420711 MS.gene022461:intron
TATGTCATATCCACTTGTCC+AGG 0.562397 3.2:-67423074 None:intergenic
GCCTCTTCATCACCAACTTG+AGG 0.562952 3.2:+67418569 MS.gene022461:CDS
TCCTCAAGTTGGTGATGAAG+AGG 0.564410 3.2:-67418570 None:intergenic
ATTTGAAGAGCCCATGGGAA+GGG 0.565074 3.2:+67425048 MS.gene022461:CDS
GAGATGTATCCATTTGCATA+TGG 0.566529 3.2:-67423860 None:intergenic
CAAGACATCTCTATAGTCCC+TGG 0.567071 3.2:-67425525 None:intergenic
GAATATGCTAATTATCAACC+TGG 0.571971 3.2:+67423764 MS.gene022461:CDS
ACTGAACATGACTGGAGAGA+AGG 0.580541 3.2:+67424896 MS.gene022461:CDS
CACCACTGACCAGCTCATCA+AGG 0.584625 3.2:+67423796 MS.gene022461:CDS
ACCAGGATCGCGCCAGCCAA+CGG 0.585321 3.2:-67423306 None:intergenic
GAGTACAAGAAAAGTAGTGA+TGG 0.585752 3.2:+67425476 MS.gene022461:CDS
TGCAGGGCGAGGATACAGAA+TGG 0.587032 3.2:+67420710 MS.gene022461:intron
CATATTCGGTGACATGGGAA+AGG 0.587852 3.2:+67423576 MS.gene022461:CDS
ATGACAGTTACCTGGACAAG+TGG 0.589057 3.2:+67423064 MS.gene022461:CDS
ACCCAAACAGATCCTCAAGT+TGG 0.593043 3.2:-67418581 None:intergenic
CAGTTCCATATATGATAGCA+AGG 0.595584 3.2:+67423927 MS.gene022461:CDS
ATGGGAAGGGAAAGTTTGCA+GGG 0.596275 3.2:+67425061 MS.gene022461:CDS
CCAACTAATGAAGACTGGGT+TGG 0.597652 3.2:+67420760 MS.gene022461:CDS
TCCACCTGAAAATAGTGCAA+AGG 0.604263 3.2:-67422403 None:intergenic
GTTGAATGGGGCCCAAAGGG+TGG 0.605971 3.2:+67423115 MS.gene022461:CDS
TTTGCAGCACATCGCCCACT+CGG 0.606514 3.2:+67424989 MS.gene022461:CDS
TGAATGGCAATCTTTGAAAG+TGG 0.607314 3.2:-67418628 None:intergenic
CATTTGAAGAGCCCATGGGA+AGG 0.608262 3.2:+67425047 MS.gene022461:CDS
CATATGGCAGATCTCCTATG+TGG 0.608314 3.2:-67423844 None:intergenic
GCCTTGCTCATGGAAAGTCT+TGG 0.610045 3.2:+67422875 MS.gene022461:CDS
AAACCTGTGTGTATAAAACC+AGG 0.612380 3.2:-67423323 None:intergenic
GTTATCATATTCGGTGACAT+GGG 0.619175 3.2:+67423571 MS.gene022461:CDS
ACATGACTGGAGAGAAGGAT+CGG 0.620114 3.2:+67424901 MS.gene022461:CDS
CCAGATTCAGGTGGAGAATG+CGG 0.625344 3.2:+67424095 MS.gene022461:CDS
AATGATTGGTATGCCAAGGA+GGG 0.625443 3.2:+67425022 MS.gene022461:CDS
ATTTGGCTTTGTTCTCAGCA+GGG 0.626371 3.2:-67424145 None:intergenic
CTCACAACCATCGTGCACAC+AGG 0.626678 3.2:-67425549 None:intergenic
ACTCCAAACAGGAGGTGCTG+TGG 0.627083 3.2:-67425387 None:intergenic
TAATGATTGGTATGCCAAGG+AGG 0.627561 3.2:+67425021 MS.gene022461:CDS
AGAACCAACTAATGAAGACT+GGG 0.631293 3.2:+67420756 MS.gene022461:CDS
ATCCTTGATGAGCTGGTCAG+TGG 0.633150 3.2:-67423798 None:intergenic
GGTTCATTTGAAGAGCCCAT+GGG 0.633987 3.2:+67425043 MS.gene022461:CDS
GACTTTCCATGAGCAAGGCG+CGG 0.635264 3.2:-67422871 None:intergenic
TCAGTGGTGTTAAGTGAACC+AGG 0.637131 3.2:-67423782 None:intergenic
TTTAATCGTAACAGCATGTG+TGG 0.639129 3.2:+67423172 MS.gene022461:CDS
TCAACATGTCCACCCAACCC+CGG 0.639236 3.2:+67422135 MS.gene022461:CDS
TATGACACTCCAGATTCAGG+TGG 0.641435 3.2:+67424086 MS.gene022461:CDS
CATAATTCAGAGTGCCAAGG+TGG 0.647479 3.2:-67425576 None:intergenic
GCTGTACCATTTGTTGAATG+GGG 0.649227 3.2:+67423103 MS.gene022461:CDS
ACGAAACATGCCATAATCTG+TGG 0.650821 3.2:-67424858 None:intergenic
TAGATAGAAAACAACAACCA+TGG 0.654457 3.2:+67424960 MS.gene022461:CDS
AAATGGATACATCTCACAGT+GGG 0.656289 3.2:+67423868 MS.gene022461:CDS
CTACATAATTCAGAGTGCCA+AGG 0.658085 3.2:-67425579 None:intergenic
GTCTTGGCCTGTGTGCACGA+TGG 0.663436 3.2:+67425542 MS.gene022461:CDS
GTAGTTATACCTGATAAATG+GGG 0.675933 3.2:-67425150 None:intergenic
CATTAGAGGAGTAACCGAGT+GGG 0.692825 3.2:-67425003 None:intergenic
CTGAAAATACTCCAAACAGG+AGG 0.700173 3.2:-67425395 None:intergenic
TGGGAAGGGAAAGTTTGCAG+GGG 0.702638 3.2:+67425062 MS.gene022461:CDS
TCATTAGAGGAGTAACCGAG+TGG 0.714971 3.2:-67425004 None:intergenic
TGGTTGTGAGAAAACCACCT+TGG 0.719747 3.2:+67425562 MS.gene022461:CDS
ACATGTCCACCCAACCCCGG+TGG 0.724016 3.2:+67422138 MS.gene022461:CDS
CTACCCGTCCAATGCCACCG+GGG 0.725369 3.2:-67422152 None:intergenic
CAAATGGATACATCTCACAG+TGG 0.732996 3.2:+67423867 MS.gene022461:CDS
TGTGCTGCAAAGATTAACCA+TGG 0.735965 3.2:-67424977 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.2 gene 67418532 67425592 67418532 ID=MS.gene022461
chr3.2 mRNA 67418532 67425592 67418532 ID=MS.gene022461.t1;Parent=MS.gene022461
chr3.2 exon 67418532 67418717 67418532 ID=MS.gene022461.t1.exon1;Parent=MS.gene022461.t1
chr3.2 CDS 67418532 67418717 67418532 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67420715 67420806 67420715 ID=MS.gene022461.t1.exon2;Parent=MS.gene022461.t1
chr3.2 CDS 67420715 67420806 67420715 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67422131 67422206 67422131 ID=MS.gene022461.t1.exon3;Parent=MS.gene022461.t1
chr3.2 CDS 67422131 67422206 67422131 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67422312 67422434 67422312 ID=MS.gene022461.t1.exon4;Parent=MS.gene022461.t1
chr3.2 CDS 67422312 67422434 67422312 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67422811 67422903 67422811 ID=MS.gene022461.t1.exon5;Parent=MS.gene022461.t1
chr3.2 CDS 67422811 67422903 67422811 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67423064 67423193 67423064 ID=MS.gene022461.t1.exon6;Parent=MS.gene022461.t1
chr3.2 CDS 67423064 67423193 67423064 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67423291 67423372 67423291 ID=MS.gene022461.t1.exon7;Parent=MS.gene022461.t1
chr3.2 CDS 67423291 67423372 67423291 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67423465 67423597 67423465 ID=MS.gene022461.t1.exon8;Parent=MS.gene022461.t1
chr3.2 CDS 67423465 67423597 67423465 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67423743 67423948 67423743 ID=MS.gene022461.t1.exon9;Parent=MS.gene022461.t1
chr3.2 CDS 67423743 67423948 67423743 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67424046 67424171 67424046 ID=MS.gene022461.t1.exon10;Parent=MS.gene022461.t1
chr3.2 CDS 67424046 67424171 67424046 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67424853 67425162 67424853 ID=MS.gene022461.t1.exon11;Parent=MS.gene022461.t1
chr3.2 CDS 67424853 67425162 67424853 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
chr3.2 exon 67425287 67425592 67425287 ID=MS.gene022461.t1.exon12;Parent=MS.gene022461.t1
chr3.2 CDS 67425287 67425592 67425287 ID=cds.MS.gene022461.t1;Parent=MS.gene022461.t1
Gene Sequence

>MS.gene022461

ATGGAAGGTAAGTTAAACATGAGTTTGGTAATATTGTGCCTCTTCATCACCAACTTGAGGATCTGTTTGGGTGATATGAATGGATTTGGAGAGCAGCCACTTTCAAAGATTGCCATTCACAAAACCATTCTTGCGATCCATGATTCTGCCTCCGTTACTGCTTCCCCCTTTTTGCTTGGCAGTAAGGTAGTATCTTCTACCCCGTTTCGTTAATTTAACCTATTTATTTTGTTCTCACTAGCATGACATTAACATGCATGTTTTTCCACTTTTATTAGCAATGAATGATAATATGTTACAAAACAATTAAGTGAAATGTTAAATTGGAAGAAAAAATGTTACACCAAAATAGTTAAGTAATTCATTAATAGAAATTTTGTCTTGTAAATATAAGCTTTTACTTTTGTCTTAGTAATAATCACACCATTTTTTGATACAAACTTACTAATTTTAACACTTTAACCAATATCTCAAGGGCATTCATTAGCATTCTCCTTCCTTTATATATTGATATAGATTTATCAACAAATGTTTTGATTAAAAAAAATAACTATATTATTTTTTGAGAGAACTAATGAAATTTAGTTTTTTGAGGGGAGAATTAATGAAAGTTAAATAGTACTTGTTTCCCTCAACAAAACAAAACAGAAAATAGTACTTGATCAAGACATTTTATTTCTGCACAACTGCACCCCTTGATTTTACTTTCTACACCTGTTTGAGGCGATTTGAGCTTGGTATTTTTCTCCAGCCAGAGAAAAAACGATACAATTACTGTAATTTTAGCTAGTACTAGCATGAAAATAGTTTTTTTTTTTTTAGAGAAGCATGAAAATAGTTTAAAAGATGCAAAATTGTTTGCATGTAGCTCATAAATCTTTTATATGTTCGGTTTTCGTTGATTAGTGTCACATTTATTAAAATAACTATAATTTAAAATAAATCTTGTTTTTAATATTTTATAGGCTTAAATGCGCTTTTGGTCCCTTAACTATTTATTTGATATCGCTTTGGTCCCTTAACTAAAAAAAAGATTGTTTTGGTATTTTATCTTTATCTCCGTTACCTGTTTTGGTCCCTTCCGTTAAATAAATTCAAAAAAACGTTAGGGTTTGTACTATTCATCTTCTTCCTTCTCCTTCCATCCTGTTCTTCATCATCAACAACACACCAACCCATATTTTGGGACCAAAACGATACCAAATAAATATTAAGGGATCAAAAGAACATTTAAGTTTTTTTTTTTTTATCATCTTCTTCATCTTCATCAAAACTCATATTCATCAATTAAATCCAGAAAAAAAAAAAAAAAACACAGAATGCTCATATTTGGAAGAGTTATGCATAATGCTCAAATCAAAACTCATATTTGGCTTAAACTCATCAAAATAAAATAAAAAACACATAATGCTCAAATCAAAACTCAAATTTGGCTTAAACTCATATCAAAATCAAAACTTCAAAAGAGTTATGCATAATGCTCAAATTTGAAAAAACCTCCTTTAGAAAAACCCATCCTTCAAAAATTCGAAACAGGAGAAGAGTAAGGTTCTGCAGAATGAATTTGAAACAGGAAAAGAGTAAGATTATGCAGAATGAATTCGAAATGGGTGAAAATTAAGGTTCAAAAATTTGAGTTTTGCGAGAAGAGATTGAGGTTGTAAACAGGAGAAGATTTGAGTTTGAGATTCAACAAAGGATTACTTAAAGAAGGAGGTTTAAGTTTGAGATTCAACACAGGACACATAAGAGGAAAAGATTGAAGGATATGCTATTTTTTAGTTTTTTTTTGGATTATTTTCTGTGTTTTTTTTCTGGATTTAATTGATGAATATGAGTTTTGATGAAGATGAAGAAGAAAAACTTAAATGTTCTTTTGGTCCCTTAATTATTTATTTGGTATCGTTTTGGTCCCAAAATCTGGGTTTAGTTGATGAATGATGAAGGTCTGGGTTTAGTTGATGAATGATGAAGGTTTTGAGAAGGAAGAAGATGAATAGTACAAACCCTAACGTTTTTTTGAATTTATTTAACGGAAAGGACCAAAACAGGTAACAGAGATAAAGATAAAATACTAAAACAATCTTTTTTTTAGTTAAGGGACCAAAGCGATACCAAATAAATAGTTAAGGGACCAAAAGCGCATTTAAGCCTATTTTATAGTTGTACTCATACTATTGCAGGGCGAGGATACAGAATGGGTGACAGTAAAACTGGAGTCCCCAGAACCAACTAATGAAGACTGGGTTGGAGTTTTTACTCCTGCAAACTTCAAGTAACATACAACAATTCTCTCTCTCTCTCTCACTTTTAATATTGTTCTTTAAACACATTTTTCAAAAACATCATATCATACATATACTAAGACACTTGAGTGAATTTTGTATGAATCATATTGGTACATATTCACAATGTTAACACAGTCATGAAAAAGTACATTGACAATAGGAGTTTTTTGATTCCTCTTATGTTTTCTCTCTCTGTTTTATAATTGGAATAAAAAAAAAGAACTTGTAGTAAGTTTGCTCTCTAAAATTCAGCTCCATAACATTGCATGGTAGCCATGTGGAAAGATATGCCTTGGACTAACACAGAAGGAGTTAGTCACATGATCACACAACCTTATCTACACTTAGTCACATGAAACACCTTGGATGTAAGCAACATTCTGAATATGTCTAAAACAAAACCAACGTGAGTTAACTTACGGTGTTTGGAACGTTACTTAAGTTACGTAGTTTTTATTTCAATGTGATTTAAGTCATGTTGGGTTTAATATCTGCGCGACTTAAGTCACGTGGAGAGTATTTTAGGAAGTTTGATTGGGAGCAAGCAAATCTTGATGGGAGAAGAGCAATTTTCTTTATTTTACTTAATGACCCCTTAATTTTATGTTTGGGGTCCAAATTTTGAACTTATTTATTGGGACTGACGGTCGTCCTCCTCATCCTGCCTCAGATACGGCCCTTGTCATACCTATGTCTAGTAAACCTTATGTCATGTTCCTTAAATCCAATTTATCCAATGAGAGTTTCTTTTTTCACTCGCACGATTCTTAAGAACCGTTCGCATCTAAAAAAGGGATGGGAGGGGAGAGTCTTGAAGGAAAAAAAAATGAAGAATGAAGTGGATATAACAAAATATTTTGAAAGGGGAGGGTTTAGGAGTTTATTTTTCTTCATGGTACAAAATTCTTCCAACTTAAAACTTGTTTTTGTCGACAAATTCTAATTTAGAATTTAAAAACTTGTATTGAAGGATGATTTTGGAGGATTTTAGAAGATTAGGATAAATTTTCCAAATTTAATTTTTTTTTTTAAGGAAGGATAAATTTTCCAAATTTAATATATATTGTTATAATATTTTCAATATTAAAAATATATTAATCATAAGTATTTATTTATCATTTTCTACAAAACATTTCAAAAAAATAATCAATTCTCTAGTCTTTTCGCTACTCCCTCCTTCCCGAAGTTCTCCCATCCTTTTTCCTCCAAACTCTCCAACATCGCCTAAAGAGTCATGGCCATAAACTATGCATATATCTCACTATTATGGCTGAAAAACGAACTAAAGGAAATATATTATTGAAATCACAGTTCGTCAACATGTCCACCCAACCCCGGTGGCATTGGACGGGTAGATACTCCATTCATATGCTCAGCCCCAATAAAGGTAAATGAAACTCCATCTCATATAAATTGTGTATGAAATGAATGAACAACATAACAAGGATGAACCTCTCATGTGTGTTGATTATAATTTGAATCTTTGTAATAGTACAAGTATGCCAACCACTCCAATCCTAAATACAAAAAGACTGGGAAAACTACTTTGAAGTTCCAATTGATCAATCAACGTGCAGATTTCTCCTTTGCACTATTTTCAGGTGGATTATCCAATGTAAGTCATATCTACTGATCTTTTTTTTTTTTTTTTACTATCTTTAAATTTTTTTGTCTTTGCTTAGATGTAGATTTCACATTTAAAAAATCAGTGTCATATACTAGTGCTATTAGAGAGGGGTAACCTTGGCGCAACTGGTAAAGTTGTTGTCATGTGACTGAAAGGTCACGGGTTCAAGTCCTGGAAACAGCCTCTTGTGTAAAACAGGGTAAGGCTGTGTACAATACACCAAATGGTGGGACCCCTTCCCAGACCCTGCATATGCGAGAGCTCTAGTGCACCGGGTTGCCCCTTTATATACTAGTGCTATTAGTATGCAATGATCCTTATTCAATTGTATGCTAACAAGTGTTCATTTGATTGAGCATTGCAGCCTAAGCTTGTTGCAGTTTCAAACTTCATTGCATTTGCAAATCCTAAAGCACCTGTGTATCCGCGCCTTGCTCATGGAAAGTCTTGGAATGAAGTAAGTTTATCTAGTCAATTTAATTGCATACTAGTTGCCTAACATTTCCTAATTATACATTGGTTCTTATCATCTAAAACTTTTGTAATTGAAATTGCTGCATGAATCGTCTAACTTAAACGTTTAGAATAATGATAAGAAATTTGTTCTTCTATGACAGATGACAGTTACCTGGACAAGTGGATATGACATAAGTGAAGCTGTACCATTTGTTGAATGGGGCCCAAAGGGTGGAAAGCAGATTCAATCTGCTGCTGGAACATTGACATTTAATCGTAACAGCATGTGTGGTATGGATTAATACATCGTAACTCAATTTCATACCTTATTATCTTTTTCTAAAAATGATTCTCAACATATAATGAGGGACATTTTGTTAATCTGTAGGTCAACCTGCGCGAACCGTTGGCTGGCGCGATCCTGGTTTTATACACACAGGTTTCCTTGAAGAACTTTGGCCAAATATGAGGTGTGCATTAAACTATATTTATGTTTATGAGACTATAATTGTTAAGTAGAGTTCTGAAAGCTTAGCTACCTTCCCTCCCTATTCTTCCCCAGGTACGCCTATAGGTTGGGGCATTTATTGTCTGATGGCTCTTATGTATGGAGTAAGAGGTATTCATTCAAGGCATCTCCTTATCCTGGACAAAACTCATTGCAACGTGTTATCATATTCGGTGACATGGGAAAGGTAGCGTCGATTATTTAATGTTTATAACATTGGAAAGATTGATCACCTAACTAGATAATTTTCCAACTTTTTATCTAAAAACTCTAATTTGAAACTTGATTTGTACAATGGGGAGAACACTTATTTTGGTTCACATGTCCTTTAGGCTGAGCGTGATGGTTCAAATGAATATGCTAATTATCAACCTGGTTCACTTAACACCACTGACCAGCTCATCAAGGATTTAGACAATATTGACATTGTTTTCCACATAGGAGATCTGCCATATGCAAATGGATACATCTCACAGTGGGATCAATTCACAGCTCAAGTACAACAAATTTCATCAACAGTTCCATATATGATAGCAAGGTTCGCTTGCTTGCAATCATTTTAGGCCATAGAAATTTGAATTGAGTTTTACATATTCTTTTAGACTGAAAGGATATCACATTTATTCATACTGCAGTGGAAATCACGAACGCTCTTGGCCAAACTCAGGATCCTTCTATGACACTCCAGATTCAGGTGGAGAATGCGGAGTTCCTGCAGAAACCATGTACTATTACCCTGCTGAGAACAAAGCCAAATTCTGGTGAGTTATATTAATCTAAATGTAAACCATGACATGTTTAGCATTAATAAAACAGATTTTCTTTTCTCAACTGAATTTTTCACTACTTGGTCCAAGATAGTACGTATGATCCTGACCAATGGTTGCGTAATTATTTCTATAATTTCATTGTTGGAACAAAGTATGGTTTTGTGTTGATTTTTGAAAGAATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGATTGAGATTAAGAGATTAAGAGTTTGAGAATAATGAGAGGAGTAATATTGCTTTCTCAAATTCAGTTACAATATGCATACAACATAGAAGCTATTTATAGGCATGGTTTTGTTACAATAGATTGCTTAACACGCAAGCAATGCATATAACAAACCCTAACTGTTTTATTTGCAAGCTAAACAAACTTAAAACTAACTTCTAACAAACTTAACAAACTGAATTAACACATTCCAGCATTCATGATATCTGCAAAAGATACATTTTCATCCTTATAGTTCATGGAATCATGGTGCAGTTTCTAGTTTTTGCAAGTGAAAAATAAACTAGGAGGTACTATTTTTCCATTCCTAATTAATGACGAAAATTGGAAATATGCAGGTATGCCACAGATTATGGCATGTTTCGTTTCTGTATAGCAGACACTGAACATGACTGGAGAGAAGGATCGGAACAGTACAAATTCATCGAGCATTGCCTTGCAACAGTAGATAGAAAACAACAACCATGGTTAATCTTTGCAGCACATCGCCCACTCGGTTACTCCTCTAATGATTGGTATGCCAAGGAGGGTTCATTTGAAGAGCCCATGGGAAGGGAAAGTTTGCAGGGGCTTTGGCAAAAGTATAAAGTAGACATTGCATTTTATGGTCACGTCCATAACTACGAAAGAGTATGCCCCATTTATCAGGTATAACTACTATTGTCACATTTCAGTTAAAGCGAGATGTGCTGTCTATATTAAGTACTTGATTGTTACAGTTTTTACCGACAGCCTACATCCATTTCTAATTATCCAAATGTGTATAATGCAGAACCAATGCGTGAATAAAGAAAAATCTCATTATTCTGGCACTGTGAATGGAACAATTCATGTTGTTGTTGGTGGAGGGGGAAGTCACTTGTCAGACTTCACCACAGCACCTCCTGTTTGGAGTATTTTCAGAGACAAAGACTATGGTTTTGGCAAACTGACAGCATTTAATCACTCATATCTCTTGTTTGAGTACAAGAAAAGTAGTGATGGAAAGGTCTATGATTCTTTTACCATTTCCAGGGACTATAGAGATGTCTTGGCCTGTGTGCACGATGGTTGTGAGAAAACCACCTTGGCACTCTGA
Protein sequence

>MS.gene022461.t1

MEGKLNMSLVILCLFITNLRICLGDMNGFGEQPLSKIAIHKTILAIHDSASVTASPFLLGSKGEDTEWVTVKLESPEPTNEDWVGVFTPANFNSSTCPPNPGGIGRVDTPFICSAPIKYKYANHSNPKYKKTGKTTLKFQLINQRADFSFALFSGGLSNPKLVAVSNFIAFANPKAPVYPRLAHGKSWNEMTVTWTSGYDISEAVPFVEWGPKGGKQIQSAAGTLTFNRNSMCGQPARTVGWRDPGFIHTGFLEELWPNMRYAYRLGHLLSDGSYVWSKRYSFKASPYPGQNSLQRVIIFGDMGKAERDGSNEYANYQPGSLNTTDQLIKDLDNIDIVFHIGDLPYANGYISQWDQFTAQVQQISSTVPYMIASGNHERSWPNSGSFYDTPDSGGECGVPAETMYYYPAENKAKFWYATDYGMFRFCIADTEHDWREGSEQYKFIEHCLATVDRKQQPWLIFAAHRPLGYSSNDWYAKEGSFEEPMGRESLQGLWQKYKVDIAFYGHVHNYERVCPIYQNQCVNKEKSHYSGTVNGTIHVVVGGGGSHLSDFTTAPPVWSIFRDKDYGFGKLTAFNHSYLLFEYKKSSDGKVYDSFTISRDYRDVLACVHDGCEKTTLAL