AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023307

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023307.t1 MTR_0332s0040 100.000 187 0 0 1 187 1 187 4.28e-138 392
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023307.t1 AT1G73820 78.238 193 42 0 1 193 1 193 2.66e-119 336
MS.gene023307.t1 AT1G73820 78.238 193 42 0 1 193 17 209 2.66e-119 337

Find 36 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAAACATGGTCTCTATTA+TGG 0.222869 4.2:-15727765 None:intergenic
AACGGTATTGATAGTCAATT+TGG 0.243908 4.2:+15727792 MS.gene023307:CDS
GAATCCTGCCAATGCTTAAA+AGG 0.252032 4.2:+15726291 MS.gene023307:CDS
GAACCAAATGTCTATGAATT+TGG 0.253761 4.2:+15725072 MS.gene023307:CDS
GGGTTCGATGTATCTTCTTA+TGG 0.256941 4.2:+15725009 MS.gene023307:CDS
GTGCTTCCATGCTTCTGTTT+TGG 0.284804 4.2:-15724966 None:intergenic
AAGGAATGCAGCTGTGAAAT+TGG 0.321236 4.2:+15726310 MS.gene023307:CDS
GTACCAAATTCATAGACATT+TGG 0.395982 4.2:-15725075 None:intergenic
TAAAGTCTGTCTTGCTCCTA+TGG 0.406647 4.2:-15727842 None:intergenic
GATGTATCTTCTTATGGAAC+AGG 0.408399 4.2:+15725015 MS.gene023307:CDS
GAGTGGAGCTACAAGCGAAA+TGG 0.467833 4.2:+15726269 MS.gene023307:intron
AGATTGAAGCTGTTGAATCA+TGG 0.474304 4.2:+15728078 MS.gene023307:intron
GTATTTGATATGGTTGTTGA+AGG 0.475037 4.2:+15726401 MS.gene023307:CDS
ACTAGCAATAACATCATCAA+TGG 0.487232 4.2:-15728108 None:intergenic
TCATCAAGCATATATTTGTA+AGG 0.512106 4.2:-15725099 None:intergenic
TGAAATTGGCACCTCAAAGA+TGG 0.526468 4.2:+15726324 MS.gene023307:CDS
GATTGAAGCTGTTGAATCAT+GGG 0.531323 4.2:+15728079 MS.gene023307:intron
GGTTCTCTGAGGGAAGGACC+AGG 0.532924 4.2:-15725054 None:intergenic
GGTATTGATAGTCAATTTGG+AGG 0.563161 4.2:+15727795 MS.gene023307:CDS
GGTACCCACGTTAAGCTCCC+TGG 0.567847 4.2:+15725036 MS.gene023307:CDS
CATAGACATTTGGTTCTCTG+AGG 0.569922 4.2:-15725065 None:intergenic
ATAGACATTTGGTTCTCTGA+GGG 0.571121 4.2:-15725064 None:intergenic
ATCCAACCAAAACAGAAGCA+TGG 0.589136 4.2:+15724960 MS.gene023307:CDS
AATGAAGTTTCGTTACGCCA+TGG 0.597155 4.2:+15724930 None:intergenic
AAGGACCAGGGAGCTTAACG+TGG 0.600033 4.2:-15725041 None:intergenic
ACATTTGGTTCTCTGAGGGA+AGG 0.600394 4.2:-15725060 None:intergenic
AACCACGAGGAAGCCGCCAT+AGG 0.612265 4.2:+15727826 MS.gene023307:CDS
TGGCAAGAAAATGCTGCTGA+TGG 0.614390 4.2:+15726344 MS.gene023307:CDS
CTCCTATGGCGGCTTCCTCG+TGG 0.618433 4.2:-15727828 None:intergenic
GTTCTCTGAGGGAAGGACCA+GGG 0.632137 4.2:-15725053 None:intergenic
AGTCTGTCTTGCTCCTATGG+CGG 0.641994 4.2:-15727839 None:intergenic
TTGGTTGGATGAACAAACCA+TGG 0.652936 4.2:-15724947 None:intergenic
TGAAGCTGTTGAATCATGGG+AGG 0.684017 4.2:+15728082 MS.gene023307:CDS
TGCTGATGGCATATTTGATG+TGG 0.692771 4.2:+15726358 MS.gene023307:CDS
AGGACCAGGGAGCTTAACGT+GGG 0.701054 4.2:-15725040 None:intergenic
GGAGGTCAAAGATAACCACG+AGG 0.849826 4.2:+15727813 MS.gene023307:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATTAAGTAAACAAA+AGG - chr4.2:15725180-15725199 None:intergenic 10.0%
!! AAATAATTAAGTAAACAAAA+GGG - chr4.2:15725179-15725198 None:intergenic 10.0%
!! AATGTGATATAAAAAAAAAA+GGG - chr4.2:15727442-15727461 None:intergenic 10.0%
!! TAAAAAATGCAAATAAAAAA+AGG - chr4.2:15725153-15725172 None:intergenic 10.0%
!!! AAATTTAAGTTTTAGTTTTA+TGG + chr4.2:15725306-15725325 MS.gene023307:intron 10.0%
!!! AATTTAAGTTTTAGTTTTAT+GGG + chr4.2:15725307-15725326 MS.gene023307:intron 10.0%
!!! GAGTTATATATTTTTAATTT+TGG + chr4.2:15727993-15728012 MS.gene023307:intron 10.0%
!!! TACTTAATTATTTTAGTTTT+AGG + chr4.2:15725186-15725205 MS.gene023307:intron 10.0%
!!! TTTTGTTTTAAAAGAATTTT+AGG + chr4.2:15725695-15725714 MS.gene023307:intron 10.0%
!! AAAATGAAAATTTAGATTTG+GGG + chr4.2:15725624-15725643 MS.gene023307:intron 15.0%
!! AAATTAAAAACAAGCAAAAT+TGG - chr4.2:15725355-15725374 None:intergenic 15.0%
!! CAATGTGATATAAAAAAAAA+AGG - chr4.2:15727443-15727462 None:intergenic 15.0%
!! GAAAATGAAAATTTAGATTT+GGG + chr4.2:15725623-15725642 MS.gene023307:intron 15.0%
!! TGAAAATGAAAATTTAGATT+TGG + chr4.2:15725622-15725641 MS.gene023307:intron 15.0%
!!! ATTTAAGTTTTAGTTTTATG+GGG + chr4.2:15725308-15725327 MS.gene023307:intron 15.0%
!!! GATTTTACTATTTAAGTTAT+TGG + chr4.2:15727587-15727606 MS.gene023307:intron 15.0%
!! AAATGAAAATTTAGATTTGG+GGG + chr4.2:15725625-15725644 MS.gene023307:intron 20.0%
!! AGACATGAAAATATTAAAAC+AGG - chr4.2:15728028-15728047 None:intergenic 20.0%
!! TGATGATACTAAGAAATATT+GGG + chr4.2:15726117-15726136 MS.gene023307:intron 20.0%
!! TGATGTTATAAATAAGTGAT+TGG + chr4.2:15726925-15726944 MS.gene023307:intron 20.0%
!!! AGATTTGTGTATTTTTCATA+AGG + chr4.2:15725433-15725452 MS.gene023307:intron 20.0%
!!! ATTAACATTAATTTGTCTAC+AGG + chr4.2:15725545-15725564 MS.gene023307:intron 20.0%
!!! TGAAGAAAAAGTATTTGATA+TGG + chr4.2:15726391-15726410 MS.gene023307:CDS 20.0%
!!! TTTAATATTTTCATGTCTCA+TGG + chr4.2:15728030-15728049 MS.gene023307:intron 20.0%
!!! TTTAGTTTTAGTAGAATTTC+AGG + chr4.2:15725377-15725396 MS.gene023307:intron 20.0%
!!! TTTTTCAGACAAAAAGTTAA+GGG + chr4.2:15727624-15727643 MS.gene023307:intron 20.0%
!!! TTTTTTCAGACAAAAAGTTA+AGG + chr4.2:15727623-15727642 MS.gene023307:intron 20.0%
! AAAAGGAGTAAAAAAGACAA+AGG - chr4.2:15726432-15726451 None:intergenic 25.0%
! AATGAAAATTTAGATTTGGG+GGG + chr4.2:15725626-15725645 MS.gene023307:intron 25.0%
! ATAAAAAAAGGATACAGTTC+AGG - chr4.2:15725141-15725160 None:intergenic 25.0%
! ATAAAACATGGTCTCTATTA+TGG - chr4.2:15727768-15727787 None:intergenic 25.0%
! ATTAATGTTAATATCGTGTC+TGG - chr4.2:15725537-15725556 None:intergenic 25.0%
! CTATCCATAAAATATATCCA+TGG - chr4.2:15728060-15728079 None:intergenic 25.0%
! CTGATGATACTAAGAAATAT+TGG + chr4.2:15726116-15726135 MS.gene023307:intron 25.0%
! GATGATACTAAGAAATATTG+GGG + chr4.2:15726118-15726137 MS.gene023307:intron 25.0%
! TAAAAAAAGGATACAGTTCA+GGG - chr4.2:15725140-15725159 None:intergenic 25.0%
! TAACTAATGAGTCATCATAA+TGG - chr4.2:15726891-15726910 None:intergenic 25.0%
! TACAAAACAAGAATTCGAAA+TGG - chr4.2:15726223-15726242 None:intergenic 25.0%
! TAGGATTTAGTGAATAGTTT+GGG + chr4.2:15725783-15725802 MS.gene023307:intron 25.0%
! TCAGCTAAAAAAAATCGAAA+GGG - chr4.2:15725605-15725624 None:intergenic 25.0%
! TCATAAGGATTTATGATGAT+AGG + chr4.2:15725448-15725467 MS.gene023307:intron 25.0%
! TCATCAAGCATATATTTGTA+AGG - chr4.2:15725102-15725121 None:intergenic 25.0%
! TTAGATCAATCAAAGAGAAA+AGG - chr4.2:15726449-15726468 None:intergenic 25.0%
! TTAGGATTTAGTGAATAGTT+TGG + chr4.2:15725782-15725801 MS.gene023307:intron 25.0%
! TTCAGCTAAAAAAAATCGAA+AGG - chr4.2:15725606-15725625 None:intergenic 25.0%
! TTCAGCTAAAACAATCAAAA+GGG - chr4.2:15725288-15725307 None:intergenic 25.0%
! TTTCAGCTAAAACAATCAAA+AGG - chr4.2:15725289-15725308 None:intergenic 25.0%
!! AACTGTTATGAACAACTTTT+TGG - chr4.2:15727060-15727079 None:intergenic 25.0%
!! ACTTTCTTTTCAAAAGACAA+GGG + chr4.2:15724989-15725008 MS.gene023307:CDS 25.0%
!! AGTTGCATTTTATATCTTAG+TGG + chr4.2:15726828-15726847 MS.gene023307:intron 25.0%
!! ATACCGTTTTCAATAAAACA+TGG - chr4.2:15727780-15727799 None:intergenic 25.0%
!! ATGTTTAGAGTAGTTAGTTA+TGG + chr4.2:15726989-15727008 MS.gene023307:intron 25.0%
!! CTTACCATGGATATATTTTA+TGG + chr4.2:15728053-15728072 MS.gene023307:intron 25.0%
!! TGTATAATCTATGAAGTACT+GGG + chr4.2:15725816-15725835 MS.gene023307:intron 25.0%
!!! AGACCATGTTTTATTGAAAA+CGG + chr4.2:15727774-15727793 MS.gene023307:CDS 25.0%
!!! TTTCGAATTCTTGTTTTGTA+TGG + chr4.2:15726223-15726242 MS.gene023307:intron 25.0%
!!! TTTTTTCTTAAGCATAAGTG+AGG + chr4.2:15725209-15725228 MS.gene023307:intron 25.0%
ACTAGCAATAACATCATCAA+TGG - chr4.2:15728111-15728130 None:intergenic 30.0%
AGGATTTATGATGATAGGTT+AGG + chr4.2:15725453-15725472 MS.gene023307:intron 30.0%
ATAAAAAAAAAAGGGCAACC+CGG - chr4.2:15727434-15727453 None:intergenic 30.0%
ATTGTAGAGAAAACTCTTTG+AGG + chr4.2:15726546-15726565 MS.gene023307:intron 30.0%
CTACATAGTTGCAATTGTTA+TGG + chr4.2:15726681-15726700 MS.gene023307:intron 30.0%
GAACATAGATACTAATGCAA+TGG + chr4.2:15727944-15727963 MS.gene023307:intron 30.0%
GAACCAAATGTCTATGAATT+TGG + chr4.2:15725072-15725091 MS.gene023307:CDS 30.0%
GAATTGTGTGTTTCTCATAA+AGG + chr4.2:15725744-15725763 MS.gene023307:intron 30.0%
GTACCAAATTCATAGACATT+TGG - chr4.2:15725078-15725097 None:intergenic 30.0%
GTATTTGATATGGTTGTTGA+AGG + chr4.2:15726401-15726420 MS.gene023307:CDS 30.0%
GTGAAGACGAATAAAAAGTT+TGG - chr4.2:15726521-15726540 None:intergenic 30.0%
GTGTATAATCTATGAAGTAC+TGG + chr4.2:15725815-15725834 MS.gene023307:intron 30.0%
TAATCAGTAGCAAGATACAT+AGG + chr4.2:15726735-15726754 MS.gene023307:intron 30.0%
TAGGATTGAATGCATAGTAT+GGG + chr4.2:15725472-15725491 MS.gene023307:intron 30.0%
TGAATAGTTTGGGAAAAAGA+TGG + chr4.2:15725793-15725812 MS.gene023307:intron 30.0%
TGTTTCTCATAAAGGATTGA+GGG + chr4.2:15725752-15725771 MS.gene023307:intron 30.0%
TTAGGATTGAATGCATAGTA+TGG + chr4.2:15725471-15725490 MS.gene023307:intron 30.0%
TTTCATATACAACACTTTCG+TGG + chr4.2:15727532-15727551 MS.gene023307:intron 30.0%
TTTCTGTAACGAATCAAGAT+AGG + chr4.2:15726758-15726777 MS.gene023307:intron 30.0%
! AATAATTGAGTTGTCAGTGT+CGG + chr4.2:15725905-15725924 MS.gene023307:intron 30.0%
! ACAGACTTTAGATTTTTGTC+AGG + chr4.2:15727855-15727874 MS.gene023307:CDS 30.0%
! ATTTTGTAATTGAGGATGAG+GGG + chr4.2:15726141-15726160 MS.gene023307:intron 30.0%
! CACTTTCTTTTCAAAAGACA+AGG + chr4.2:15724988-15725007 MS.gene023307:CDS 30.0%
! GATTTTGTAATTGAGGATGA+GGG + chr4.2:15726140-15726159 MS.gene023307:intron 30.0%
! TACAGGAAGATGTGTATTTT+AGG + chr4.2:15725562-15725581 MS.gene023307:intron 30.0%
! TAGGCCTAAACTATTTTCTT+GGG - chr4.2:15726784-15726803 None:intergenic 30.0%
! TATTGGGGATTTTGTAATTG+AGG + chr4.2:15726133-15726152 MS.gene023307:intron 30.0%
! TGTTATATCACATTGATAGC+AGG + chr4.2:15727241-15727260 MS.gene023307:intron 30.0%
! TTAGGCCTAAACTATTTTCT+TGG - chr4.2:15726785-15726804 None:intergenic 30.0%
!! AACGGTATTGATAGTCAATT+TGG + chr4.2:15727792-15727811 MS.gene023307:CDS 30.0%
!! TAAAGTTGTTTTCATGTGAC+TGG + chr4.2:15727287-15727306 MS.gene023307:intron 30.0%
!!! CTAGTTTTGAGAAACAACAT+CGG + chr4.2:15728126-15728145 MS.gene023307:CDS 30.0%
!!! TCTTGTTTTGTATGGTGAAA+TGG + chr4.2:15726231-15726250 MS.gene023307:intron 30.0%
AAGTTAAGGGCAAGATACAT+AGG + chr4.2:15727637-15727656 MS.gene023307:intron 35.0%
AGATTGAAGCTGTTGAATCA+TGG + chr4.2:15728078-15728097 MS.gene023307:intron 35.0%
AGCCTCTTGTGTAAAAAACA+GGG + chr4.2:15727337-15727356 MS.gene023307:intron 35.0%
ATCATAGTCCTTAGCTACTT+AGG - chr4.2:15726803-15726822 None:intergenic 35.0%
ATCTGACACTGGTAGTAATT+TGG + chr4.2:15725853-15725872 MS.gene023307:intron 35.0%
ATGCATAGTATGGGAAACAT+TGG + chr4.2:15725481-15725500 MS.gene023307:intron 35.0%
CAAAATTGCCAGCATATCAA+AGG - chr4.2:15726081-15726100 None:intergenic 35.0%
CTTGTGTAAAAAACAGGGTA+AGG + chr4.2:15727342-15727361 MS.gene023307:intron 35.0%
GATGTATCTTCTTATGGAAC+AGG + chr4.2:15725015-15725034 MS.gene023307:CDS 35.0%
GATTGAAGCTGTTGAATCAT+GGG + chr4.2:15728079-15728098 MS.gene023307:intron 35.0%
GTGTATAATCTACGAAGTTC+TGG + chr4.2:15725503-15725522 MS.gene023307:intron 35.0%
GTGTTTCTCATAAAGGATTG+AGG + chr4.2:15725751-15725770 MS.gene023307:intron 35.0%
GTTAATATCGTGTCTGGTAT+CGG - chr4.2:15725531-15725550 None:intergenic 35.0%
TAGGCCCAAGAAAATAGTTT+AGG + chr4.2:15726777-15726796 MS.gene023307:intron 35.0%
TAGTAATTTGGGAATGACTC+TGG + chr4.2:15725865-15725884 MS.gene023307:intron 35.0%
TCACTTTCGATCAGAAGTAA+CGG + chr4.2:15725937-15725956 MS.gene023307:intron 35.0%
TCTGACACTGGTAGTAATTT+GGG + chr4.2:15725854-15725873 MS.gene023307:intron 35.0%
! ATATCACATTGATAGCAGGA+GGG + chr4.2:15727245-15727264 MS.gene023307:intron 35.0%
! CTTTTAACAGAACATGCGTT+TGG + chr4.2:15727174-15727193 MS.gene023307:intron 35.0%
! GACTTTAGATTTTTGTCAGG+AGG + chr4.2:15727858-15727877 MS.gene023307:CDS 35.0%
! GAGTCATATCATGTTCTAGA+AGG + chr4.2:15726853-15726872 MS.gene023307:intron 35.0%
! GGATTTTGTAATTGAGGATG+AGG + chr4.2:15726139-15726158 MS.gene023307:intron 35.0%
! TATATCACATTGATAGCAGG+AGG + chr4.2:15727244-15727263 MS.gene023307:intron 35.0%
! TGATATGCTGGCAATTTTGT+TGG + chr4.2:15726082-15726101 MS.gene023307:intron 35.0%
!! ATAGACATTTGGTTCTCTGA+GGG - chr4.2:15725067-15725086 None:intergenic 35.0%
!! GGTATTGATAGTCAATTTGG+AGG + chr4.2:15727795-15727814 MS.gene023307:CDS 35.0%
!!! TACCCTGTTTTTTACACAAG+AGG - chr4.2:15727342-15727361 None:intergenic 35.0%
AAGGAATGCAGCTGTGAAAT+TGG + chr4.2:15726310-15726329 MS.gene023307:CDS 40.0%
AGTTTAGGCCTAAGTAGCTA+AGG + chr4.2:15726792-15726811 MS.gene023307:intron 40.0%
ATCCAACCAAAACAGAAGCA+TGG + chr4.2:15724960-15724979 MS.gene023307:CDS 40.0%
CAGCCTCTTGTGTAAAAAAC+AGG + chr4.2:15727336-15727355 MS.gene023307:intron 40.0%
CATAAAGGATTGAGGGTGAT+AGG + chr4.2:15725759-15725778 MS.gene023307:intron 40.0%
CGTACAATACACCAAATGGT+GGG + chr4.2:15727368-15727387 MS.gene023307:intron 40.0%
CTTCTGATCGAAAGTGAATC+AGG - chr4.2:15725934-15725953 None:intergenic 40.0%
GAATCCTGCCAATGCTTAAA+AGG + chr4.2:15726291-15726310 MS.gene023307:CDS 40.0%
GCAAGATACATAGGTTTCTC+TGG + chr4.2:15727646-15727665 MS.gene023307:intron 40.0%
GGAATGATTTCTGTACGCTT+TGG + chr4.2:15727667-15727686 MS.gene023307:intron 40.0%
GGATGTATCCTTAACAAGAG+TGG + chr4.2:15726252-15726271 MS.gene023307:intron 40.0%
GGGTTCGATGTATCTTCTTA+TGG + chr4.2:15725009-15725028 MS.gene023307:CDS 40.0%
TAAAGTCTGTCTTGCTCCTA+TGG - chr4.2:15727845-15727864 None:intergenic 40.0%
TAATTTGGGAATGACTCTGG+TGG + chr4.2:15725868-15725887 MS.gene023307:intron 40.0%
TGAAATTGGCACCTCAAAGA+TGG + chr4.2:15726324-15726343 MS.gene023307:CDS 40.0%
TGCTGATGGCATATTTGATG+TGG + chr4.2:15726358-15726377 MS.gene023307:CDS 40.0%
TTCGAAATGGCATTTCCAAG+AGG - chr4.2:15726210-15726229 None:intergenic 40.0%
TTGGTTGGATGAACAAACCA+TGG - chr4.2:15724950-15724969 None:intergenic 40.0%
TTGTAGCTCCACTCTTGTTA+AGG - chr4.2:15726263-15726282 None:intergenic 40.0%
! ACTCTTTGAGGAGCATGTTT+CGG + chr4.2:15726558-15726577 MS.gene023307:intron 40.0%
! GAGTGTTTTGCATCTGTTGT+TGG + chr4.2:15726183-15726202 MS.gene023307:intron 40.0%
! GCATTTTCTTGCCATCTTTG+AGG - chr4.2:15726338-15726357 None:intergenic 40.0%
! GCTGCATTCCTTTTAAGCAT+TGG - chr4.2:15726302-15726321 None:intergenic 40.0%
! TATCACATTGATAGCAGGAG+GGG + chr4.2:15727246-15727265 MS.gene023307:intron 40.0%
! TTTGGAGTCACAACTTTGCA+AGG + chr4.2:15727192-15727211 MS.gene023307:intron 40.0%
!! CATAGACATTTGGTTCTCTG+AGG - chr4.2:15725068-15725087 None:intergenic 40.0%
!! GTTGTTTTCATGTGACTGGA+AGG + chr4.2:15727291-15727310 MS.gene023307:intron 40.0%
!!! CATTCCTTTTAAGCATTGGC+AGG - chr4.2:15726298-15726317 None:intergenic 40.0%
!!! GTTTTGAGAAACAACATCGG+CGG + chr4.2:15728129-15728148 MS.gene023307:CDS 40.0%
!!! TTCCATGCTTCTGTTTTGGT+TGG - chr4.2:15724965-15724984 None:intergenic 40.0%
AGGATTGAGGGTGATAGGTT+AGG + chr4.2:15725764-15725783 MS.gene023307:intron 45.0%
GCGTACAATACACCAAATGG+TGG + chr4.2:15727367-15727386 MS.gene023307:intron 45.0%
GCTGCGTACAATACACCAAA+TGG + chr4.2:15727364-15727383 MS.gene023307:intron 45.0%
TACACTGGAACATCTGACAC+TGG + chr4.2:15725842-15725861 MS.gene023307:intron 45.0%
TCATGTCTCATGGCTTACCA+TGG + chr4.2:15728040-15728059 MS.gene023307:intron 45.0%
TGAAGCTGTTGAATCATGGG+AGG + chr4.2:15728082-15728101 MS.gene023307:CDS 45.0%
TGAGGATGAGGGGTTAGATT+AGG + chr4.2:15726151-15726170 MS.gene023307:intron 45.0%
TGGCAAGAAAATGCTGCTGA+TGG + chr4.2:15726344-15726363 MS.gene023307:CDS 45.0%
TTCATGTGACTGGAAGGTCA+CGG + chr4.2:15727297-15727316 MS.gene023307:intron 45.0%
! CCAGCATATCAAAGGCACAA+CGG - chr4.2:15726073-15726092 None:intergenic 45.0%
! GTGCTTCCATGCTTCTGTTT+TGG - chr4.2:15724969-15724988 None:intergenic 45.0%
! TTACACAAGAGGCTGTTTCC+AGG - chr4.2:15727331-15727350 None:intergenic 45.0%
!! ACATTTGGTTCTCTGAGGGA+AGG - chr4.2:15725063-15725082 None:intergenic 45.0%
!! CTTTCGTGGTAGTTTGCTGA+TGG + chr4.2:15727546-15727565 MS.gene023307:intron 45.0%
!! TGAAGTACTGGGACATACAC+TGG + chr4.2:15725827-15725846 MS.gene023307:intron 45.0%
!!! TTTTTTTTTTTTTTTATCTT+TGG + chr4.2:15727127-15727146 MS.gene023307:intron 5.0%
AGTCTGTCTTGCTCCTATGG+CGG - chr4.2:15727842-15727861 None:intergenic 50.0%
CAACTTTACCAGTTGCGCCA+AGG - chr4.2:15727275-15727294 None:intergenic 50.0%
CCGTTGTGCCTTTGATATGC+TGG + chr4.2:15726070-15726089 MS.gene023307:intron 50.0%
GAGTGGAGCTACAAGCGAAA+TGG + chr4.2:15726269-15726288 MS.gene023307:intron 50.0%
GCATCTGTTGTTGGACCTCT+TGG + chr4.2:15726192-15726211 MS.gene023307:intron 50.0%
GGAGGTCAAAGATAACCACG+AGG + chr4.2:15727813-15727832 MS.gene023307:CDS 50.0%
TCATGTGACTGGAAGGTCAC+GGG + chr4.2:15727298-15727317 MS.gene023307:intron 50.0%
AAGGACCAGGGAGCTTAACG+TGG - chr4.2:15725044-15725063 None:intergenic 55.0%
AAGGTCACGGGTTCAAGTCC+TGG + chr4.2:15727310-15727329 MS.gene023307:intron 55.0%
ACTAAAGCTCCCGCATACGC+AGG - chr4.2:15727408-15727427 None:intergenic 55.0%
AGGACCAGGGAGCTTAACGT+GGG - chr4.2:15725043-15725062 None:intergenic 55.0%
CTAAAGCTCCCGCATACGCA+GGG - chr4.2:15727407-15727426 None:intergenic 55.0%
GATAGCAGGAGGGGTAACCT+TGG + chr4.2:15727255-15727274 MS.gene023307:intron 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG + chr4.2:15727412-15727431 MS.gene023307:intron 55.0%
! GTTCTCTGAGGGAAGGACCA+GGG - chr4.2:15725056-15725075 None:intergenic 55.0%
AACCACGAGGAAGCCGCCAT+AGG + chr4.2:15727826-15727845 MS.gene023307:CDS 60.0%
AGGGGTAACCTTGGCGCAAC+TGG + chr4.2:15727264-15727283 MS.gene023307:intron 60.0%
GGTACCCACGTTAAGCTCCC+TGG + chr4.2:15725036-15725055 MS.gene023307:CDS 60.0%
! CAAATGGTGGGACCCCTTCC+CGG + chr4.2:15727380-15727399 MS.gene023307:intron 60.0%
! GGTTCTCTGAGGGAAGGACC+AGG - chr4.2:15725057-15725076 None:intergenic 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG + chr4.2:15727413-15727432 MS.gene023307:intron 60.0%
CATACGCAGGGTCCGGGAAG+GGG - chr4.2:15727395-15727414 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG - chr4.2:15727382-15727401 None:intergenic 65.0%
CTCCTATGGCGGCTTCCTCG+TGG - chr4.2:15727831-15727850 None:intergenic 65.0%
CTTCCCGGACCCTGCGTATG+CGG + chr4.2:15727395-15727414 MS.gene023307:intron 65.0%
GCATACGCAGGGTCCGGGAA+GGG - chr4.2:15727396-15727415 None:intergenic 65.0%
TTCCCGGACCCTGCGTATGC+GGG + chr4.2:15727396-15727415 MS.gene023307:intron 65.0%
CGCATACGCAGGGTCCGGGA+AGG - chr4.2:15727397-15727416 None:intergenic 70.0%
CTCCCGCATACGCAGGGTCC+GGG - chr4.2:15727401-15727420 None:intergenic 70.0%
GCTCCCGCATACGCAGGGTC+CGG - chr4.2:15727402-15727421 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 15724931 15728181 15724931 ID=MS.gene023307
chr4.2 mRNA 15724931 15728181 15724931 ID=MS.gene023307.t1;Parent=MS.gene023307
chr4.2 exon 15724931 15725145 15724931 ID=MS.gene023307.t1.exon1;Parent=MS.gene023307.t1
chr4.2 CDS 15724931 15725145 15724931 ID=cds.MS.gene023307.t1;Parent=MS.gene023307.t1
chr4.2 exon 15726277 15726422 15726277 ID=MS.gene023307.t1.exon2;Parent=MS.gene023307.t1
chr4.2 CDS 15726277 15726422 15726277 ID=cds.MS.gene023307.t1;Parent=MS.gene023307.t1
chr4.2 exon 15727761 15727879 15727761 ID=MS.gene023307.t1.exon3;Parent=MS.gene023307.t1
chr4.2 CDS 15727761 15727879 15727761 ID=cds.MS.gene023307.t1;Parent=MS.gene023307.t1
chr4.2 exon 15728080 15728181 15728080 ID=MS.gene023307.t1.exon4;Parent=MS.gene023307.t1
chr4.2 CDS 15728080 15728181 15728080 ID=cds.MS.gene023307.t1;Parent=MS.gene023307.t1
Gene Sequence

>MS.gene023307

ATGAAGTTTCGTTACGCCATGGTTTGTTCATCCAACCAAAACAGAAGCATGGAAGCACACTTTCTTTTCAAAAGACAAGGGTTCGATGTATCTTCTTATGGAACAGGTACCCACGTTAAGCTCCCTGGTCCTTCCCTCAGAGAACCAAATGTCTATGAATTTGGTACTCCTTACAAATATATGCTTGATGAGCTTCGTAGAAAAGACCCTGAACTGTATCCTTTTTTTATTTGCATTTTTTATTACCCTTTTGTTTACTTAATTATTTTAGTTTTAGGTTTTTTCTTAAGCATAAGTGAGGTGTATAATGTGTTTAATGCTTGTTATTTACAATTTTGATTAGTTTATTGATGACCCTTTTGATTGTTTTAGCTGAAATTTAAGTTTTAGTTTTATGGGGTTGTTTCTATTGAAAAAAGTTCCAATTTTGCTTGTTTTTAATTTAATTTAGTTTTAGTAGAATTTCAGGTTTTTCTTAAGTATAAATGAGATGAAGATGAAAAGATTTGTGTATTTTTCATAAGGATTTATGATGATAGGTTAGGATTGAATGCATAGTATGGGAAACATTGGTGTATAATCTACGAAGTTCTGGCACCGATACCAGACACGATATTAACATTAATTTGTCTACAGGAAGATGTGTATTTTAGGTTTTAGTTTAATTACGACCCTTTCGATTTTTTTTAGCTGAAAATGAAAATTTAGATTTGGGGGGTTGTTTCAAATGAAAAAAGTTTCAATTTTTATGTTTGTAGAATTAATTTTGTTTTAAAAGAATTTTAGGATTTTCTTTAGCATGAAGCATGAACAGAATTGTGTGTTTCTCATAAAGGATTGAGGGTGATAGGTTAGGATTTAGTGAATAGTTTGGGAAAAAGATGGTGTATAATCTATGAAGTACTGGGACATACACTGGAACATCTGACACTGGTAGTAATTTGGGAATGACTCTGGTGGTAATTTGAGAATAGAATAATTGAGTTGTCAGTGTCGGACACCTGATTCACTTTCGATCAGAAGTAACGGTGCTACATAGTGTATAATGTTTTTAATACTTGTTTGTTGTGTGAATGTATGAAAAAGAAAATGAAATTTTCAGTTTTTTTTTTATGATTTTTGTTTTCTCTTTATTTTAGCCGTTGTGCCTTTGATATGCTGGCAATTTTGTTGGACTGATAATTTCTGATGATACTAAGAAATATTGGGGATTTTGTAATTGAGGATGAGGGGTTAGATTAGGAATGTAAAAGAGTGTTTTGCATCTGTTGTTGGACCTCTTGGAAATGCCATTTCGAATTCTTGTTTTGTATGGTGAAATGGATGTATCCTTAACAAGAGTGGAGCTACAAGCGAAATGGAATCCTGCCAATGCTTAAAAGGAATGCAGCTGTGAAATTGGCACCTCAAAGATGGCAAGAAAATGCTGCTGATGGCATATTTGATGTGGTAATTACGTTTGAAGAAAAAGTATTTGATATGGTTGTTGAAGGTAATTCCTTTGTCTTTTTTACTCCTTTTCTCTTTGATTGATCTAATTGCATGATGTTTAGAGCTGAGTGACTTTTTAGAGAAAACTTCACTTTGCCAAACTTTTTATTCGTCTTCACTTTACATTGTAGAGAAAACTCTTTGAGGAGCATGTTTCGGTTTAAAGTTTAGACGTCTATCATAGCAAATATCTTCTTTTTCTCTCTGTTATATAACATGAACTAGCTGATTCAAAAAAAAAAAAAAAAAATCACATGAACTACATAGTTGCAATTGTTATGGTTGTTGATGATGATATGTTTATTTAAATTATTAATCAGTAGCAAGATACATAGGTTTCTGTAACGAATCAAGATAGGCCCAAGAAAATAGTTTAGGCCTAAGTAGCTAAGGACTATGATGAGTTAGTTGCATTTTATATCTTAGTGGATGAGTCATATCATGTTCTAGAAGGATCTAGAGAGATCCATTATGATGACTCATTAGTTAAGTTGTTAGAATGCTGATGTTATAAATAAGTGATTGGTCATTGTAATGATCAACGCGAAAGCAAAATGAATGAAGTTAATGTTTAGAGTAGTTAGTTATGGTTTTATTCTCTCTTTTAGAATATTATCTTCTTCATTTATGAACAACCAAAAAGTTGTTCATAACAGTTTCTCACAAAAATCAGAGTTGTGCACACTTGATTACATAATGCTTCATTTTTTTTTTTTTTTTATCTTTGGTAGAAAGTGAAACTACTGTTTTAGCTTTTAACAGAACATGCGTTTGGAGTCACAACTTTGCAAGGAGTTGCAAATATCATTTCTTTTTCTCTGTTATATCACATTGATAGCAGGAGGGGTAACCTTGGCGCAACTGGTAAAGTTGTTTTCATGTGACTGGAAGGTCACGGGTTCAAGTCCTGGAAACAGCCTCTTGTGTAAAAAACAGGGTAAGGCTGCGTACAATACACCAAATGGTGGGACCCCTTCCCGGACCCTGCGTATGCGGGAGCTTTAGTGCACCGGGTTGCCCTTTTTTTTTTATATCACATTGATAGTAGATTTGCATATATAATTTGTGTTTCTGTTCAGTCATATTAATAGTAGTGTACTATTTTTTGTGTTTCATATACAACACTTTCGTGGTAGTTTGCTGATGGCTTTGTTCTACTCTTGATGATTTTACTATTTAAGTTATTGGTTGAAAGTATTTTTTTTTTCAGACAAAAAGTTAAGGGCAAGATACATAGGTTTCTCTGGAATGATTTCTGTACGCTTTGGTGAAACTACAATGAATTGAAATTAGTAATTTCTGAAACTGATAATTTACTTTTGATGTTTGTATTGTGTAGATCTCCATAATAGAGACCATGTTTTATTGAAAACGGTATTGATAGTCAATTTGGAGGTCAAAGATAACCACGAGGAAGCCGCCATAGGAGCAAGACAGACTTTAGATTTTTGTCAGGAGGTTTGTCTCTCACAGATTTCATTTGTATTCATATATGTGCAATACTTATTTTCTAAGATGATTTGAACATAGATACTAATGCAATGGTATGACAAACTTTAACTGCTCTATTAGAGTTATATATTTTTAATTTTGGAGATGAACTCCTGTTTTAATATTTTCATGTCTCATGGCTTACCATGGATATATTTTATGGATAGATTGAAGCTGTTGAATCATGGGAGGAGTCCATTGATGATGTTATTGCTAGTTTTGAGAAACAACATCGGCGGAAGCTTTTGTACAACATCTCCTTCTACTAA
Protein sequence

>MS.gene023307.t1

MKFRYAMVCSSNQNRSMEAHFLFKRQGFDVSSYGTGTHVKLPGPSLREPNVYEFGTPYKYMLDELRRKDPELYKRNGILPMLKRNAAVKLAPQRWQENAADGIFDVVITFEEKVFDMVVEDLHNRDHVLLKTVLIVNLEVKDNHEEAAIGARQTLDFCQEIEAVESWEESIDDVIASFEKQHRRKLLYNISFY