AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02480


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02480.t1 MTR_8g076780 99.206 126 1 0 1 126 70 195 3.56e-81 236
MS.gene02480.t1 MTR_2g031960 53.043 115 52 1 14 126 41 155 1.41e-30 107
MS.gene02480.t1 MTR_2g031760 61.386 101 34 1 26 126 95 190 1.63e-29 105
MS.gene02480.t1 MTR_2g064300 51.304 115 47 2 20 126 103 216 7.90e-27 99.4
MS.gene02480.t1 MTR_7g053520 55.046 109 40 2 26 126 110 217 5.40e-26 97.4
MS.gene02480.t1 MTR_6g006060 45.714 70 38 0 56 125 111 180 7.83e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02480.t1 AT4G36420 61.111 126 42 3 1 126 61 179 9.15e-39 129
MS.gene02480.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene02480.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene02480.t1 AT3G06040 63.441 93 30 1 34 126 98 186 6.98e-30 106
MS.gene02480.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene02480.t1 AT1G70190 51.639 122 53 3 6 126 92 208 1.33e-24 93.6
MS.gene02480.t1 AT4G37660 69.118 68 21 0 59 126 100 167 2.56e-24 91.7
MS.gene02480.t1 AT2G03130 48.315 89 42 1 38 126 7 91 2.52e-15 67.0
MS.gene02480.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene02480.t1 AT3G27830 52.055 73 35 0 53 125 118 190 3.27e-15 68.6
MS.gene02480.t1 AT3G27850 52.055 73 35 0 53 125 114 186 3.53e-15 68.6
MS.gene02480.t1 AT3G27840 43.210 81 46 0 45 125 112 192 1.64e-13 64.3

Find 36 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTAATGCGAGAGAAAA+AGG 0.238846 8.1:-31133719 MS.gene02480:CDS
GGGGATTATTGCTAAGTTAA+AGG 0.252904 8.1:-31133401 MS.gene02480:CDS
TGGTTCCTGGAATGGGAATA+AGG 0.262636 8.1:-31133666 MS.gene02480:CDS
AGGTGTAGGTGGAGGGAAAG+GGG 0.337630 8.1:-31133632 MS.gene02480:CDS
TTAAAGTTATTAAAGAAGTT+AGG 0.349767 8.1:-31133516 MS.gene02480:CDS
GGTTGGTGCAAAAGCTTCTA+TGG 0.362512 8.1:-31133377 MS.gene02480:CDS
GGTTCCTGGAATGGGAATAA+GGG 0.368867 8.1:-31133665 MS.gene02480:CDS
GCGTTTGATGTGAAGCTTGA+TGG 0.371046 8.1:-31133559 MS.gene02480:CDS
ATTCCCCTTATTCCCATTCC+AGG 0.371516 8.1:+31133661 None:intergenic
GGTGTAGGTGGAGGGAAAGG+GGG 0.396694 8.1:-31133631 MS.gene02480:CDS
ATTATGATGTTGATGGTTCC+TGG 0.398854 8.1:-31133679 MS.gene02480:CDS
GCTTCCGATTATGATGTTGA+TGG 0.408175 8.1:-31133686 MS.gene02480:CDS
AGAGGTGTAGGTGGAGGGAA+AGG 0.431861 8.1:-31133634 MS.gene02480:CDS
GTTGAAGGAAGCTAAGGATT+TGG 0.435359 8.1:-31133476 MS.gene02480:CDS
GGAACCATCAACATCATAAT+CGG 0.459221 8.1:+31133682 None:intergenic
GAGGTGTAGGTGGAGGGAAA+GGG 0.479166 8.1:-31133633 MS.gene02480:CDS
ATGCCGAGAGGTGTAGGTGG+AGG 0.492930 8.1:-31133640 MS.gene02480:CDS
GATGTTGATGGTTCCTGGAA+TGG 0.504794 8.1:-31133674 MS.gene02480:CDS
ATTGCTAAGTTAAAGGAGGT+TGG 0.508274 8.1:-31133394 MS.gene02480:CDS
TTTGGGGTTGAAGGAAGCTA+AGG 0.515112 8.1:-31133482 MS.gene02480:CDS
TGTGACGAAAGAAGAGGCCG+AGG 0.536944 8.1:-31133422 MS.gene02480:CDS
ATGTTGATGGTTCCTGGAAT+GGG 0.541612 8.1:-31133673 MS.gene02480:CDS
AGGGGAATGCCGAGAGGTGT+AGG 0.541839 8.1:-31133646 MS.gene02480:CDS
GAAGGAAGCTAAGGATTTGG+TGG 0.548600 8.1:-31133473 MS.gene02480:CDS
TAACTTAGCAATAATCCCCT+CGG 0.571218 8.1:+31133405 None:intergenic
GGGTGAGGTGAAGAAAGTTG+AGG 0.571665 8.1:-31133590 MS.gene02480:CDS
TTCCCTCCACCTACACCTCT+CGG 0.576289 8.1:+31133637 None:intergenic
GATTATTGCTAAGTTAAAGG+AGG 0.580906 8.1:-31133398 MS.gene02480:CDS
TGCCGAGAGGTGTAGGTGGA+GGG 0.581642 8.1:-31133639 MS.gene02480:CDS
GGAATGCCGAGAGGTGTAGG+TGG 0.612707 8.1:-31133643 MS.gene02480:CDS
ACACCTTTCTTCAAAAGCGT+AGG 0.629486 8.1:+31133442 None:intergenic
GTGACGAAAGAAGAGGCCGA+GGG 0.636034 8.1:-31133421 MS.gene02480:CDS
GTTCCTGGAATGGGAATAAG+GGG 0.637463 8.1:-31133664 MS.gene02480:CDS
GAAAGGTGTGACGAAAGAAG+AGG 0.651585 8.1:-31133428 MS.gene02480:CDS
TGACGAAAGAAGAGGCCGAG+GGG 0.703464 8.1:-31133420 MS.gene02480:CDS
GGAATAAGGGGAATGCCGAG+AGG 0.729602 8.1:-31133652 MS.gene02480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAGTTATTAAAGAAGTT+AGG - chr8.1:31133586-31133605 MS.gene02480:CDS 15.0%
GATTATTGCTAAGTTAAAGG+AGG - chr8.1:31133704-31133723 MS.gene02480:CDS 30.0%
! AGTTAGGAGTTTTACTAGTT+TGG - chr8.1:31133602-31133621 MS.gene02480:CDS 30.0%
!! GTTAGGAGTTTTACTAGTTT+GGG - chr8.1:31133603-31133622 MS.gene02480:CDS 30.0%
!! TTAGGAGTTTTACTAGTTTG+GGG - chr8.1:31133604-31133623 MS.gene02480:CDS 30.0%
ATTGCTAAGTTAAAGGAGGT+TGG - chr8.1:31133708-31133727 MS.gene02480:CDS 35.0%
GAAGTAATGCGAGAGAAAAA+GGG - chr8.1:31133384-31133403 MS.gene02480:CDS 35.0%
GGAACCATCAACATCATAAT+CGG + chr8.1:31133423-31133442 None:intergenic 35.0%
GGGGATTATTGCTAAGTTAA+AGG - chr8.1:31133701-31133720 MS.gene02480:CDS 35.0%
TAACTTAGCAATAATCCCCT+CGG + chr8.1:31133700-31133719 None:intergenic 35.0%
TGAAGTAATGCGAGAGAAAA+AGG - chr8.1:31133383-31133402 MS.gene02480:CDS 35.0%
!! ATTATGATGTTGATGGTTCC+TGG - chr8.1:31133423-31133442 MS.gene02480:CDS 35.0%
!! TTTTACTAGTTTGGGGTTGA+AGG - chr8.1:31133611-31133630 MS.gene02480:CDS 35.0%
GCTTCCGATTATGATGTTGA+TGG - chr8.1:31133416-31133435 MS.gene02480:CDS 40.0%
GTTGAAGGAAGCTAAGGATT+TGG - chr8.1:31133626-31133645 MS.gene02480:CDS 40.0%
! ACACCTTTCTTCAAAAGCGT+AGG + chr8.1:31133663-31133682 None:intergenic 40.0%
!! ATGTTGATGGTTCCTGGAAT+GGG - chr8.1:31133429-31133448 MS.gene02480:CDS 40.0%
!!! GAAGCTTGATGGTTTTGATG+CGG - chr8.1:31133554-31133573 MS.gene02480:CDS 40.0%
ATTCCCCTTATTCCCATTCC+AGG + chr8.1:31133444-31133463 None:intergenic 45.0%
GAAGGAAGCTAAGGATTTGG+TGG - chr8.1:31133629-31133648 MS.gene02480:CDS 45.0%
GCGTTTGATGTGAAGCTTGA+TGG - chr8.1:31133543-31133562 MS.gene02480:CDS 45.0%
GGTTCCTGGAATGGGAATAA+GGG - chr8.1:31133437-31133456 MS.gene02480:CDS 45.0%
GTTCCTGGAATGGGAATAAG+GGG - chr8.1:31133438-31133457 MS.gene02480:CDS 45.0%
TGGTTCCTGGAATGGGAATA+AGG - chr8.1:31133436-31133455 MS.gene02480:CDS 45.0%
! GAAAGGTGTGACGAAAGAAG+AGG - chr8.1:31133674-31133693 MS.gene02480:CDS 45.0%
! GGGGTGGTGATAGTGAAAAA+GGG - chr8.1:31133490-31133509 MS.gene02480:CDS 45.0%
! GGGTGGTGATAGTGAAAAAG+GGG - chr8.1:31133491-31133510 MS.gene02480:CDS 45.0%
! GGTGGTGATAGTGAAAAAGG+GGG - chr8.1:31133492-31133511 MS.gene02480:CDS 45.0%
! GGTTGGTGCAAAAGCTTCTA+TGG - chr8.1:31133725-31133744 MS.gene02480:CDS 45.0%
! TGATAGTGAAAAAGGGGGTG+AGG - chr8.1:31133497-31133516 MS.gene02480:CDS 45.0%
! TTTGGGGTTGAAGGAAGCTA+AGG - chr8.1:31133620-31133639 MS.gene02480:CDS 45.0%
!! GATGTTGATGGTTCCTGGAA+TGG - chr8.1:31133428-31133447 MS.gene02480:CDS 45.0%
!! TCGCCTACGCTTTTGAAGAA+AGG - chr8.1:31133657-31133676 MS.gene02480:CDS 45.0%
GGGTGAGGTGAAGAAAGTTG+AGG - chr8.1:31133512-31133531 MS.gene02480:CDS 50.0%
! GGGGGTGGTGATAGTGAAAA+AGG - chr8.1:31133489-31133508 MS.gene02480:CDS 50.0%
AGGTGTAGGTGGAGGGAAAG+GGG - chr8.1:31133470-31133489 MS.gene02480:CDS 55.0%
GAGGTGTAGGTGGAGGGAAA+GGG - chr8.1:31133469-31133488 MS.gene02480:CDS 55.0%
GGAATAAGGGGAATGCCGAG+AGG - chr8.1:31133450-31133469 MS.gene02480:CDS 55.0%
GTGACGAAAGAAGAGGCCGA+GGG - chr8.1:31133681-31133700 MS.gene02480:CDS 55.0%
TGACGAAAGAAGAGGCCGAG+GGG - chr8.1:31133682-31133701 MS.gene02480:CDS 55.0%
TGTGACGAAAGAAGAGGCCG+AGG - chr8.1:31133680-31133699 MS.gene02480:CDS 55.0%
TTCCCTCCACCTACACCTCT+CGG + chr8.1:31133468-31133487 None:intergenic 55.0%
! AGAGGTGTAGGTGGAGGGAA+AGG - chr8.1:31133468-31133487 MS.gene02480:CDS 55.0%
GGTGTAGGTGGAGGGAAAGG+GGG - chr8.1:31133471-31133490 MS.gene02480:CDS 60.0%
! AGGGGAATGCCGAGAGGTGT+AGG - chr8.1:31133456-31133475 MS.gene02480:CDS 60.0%
! ATGCCGAGAGGTGTAGGTGG+AGG - chr8.1:31133462-31133481 MS.gene02480:CDS 60.0%
! GGAATGCCGAGAGGTGTAGG+TGG - chr8.1:31133459-31133478 MS.gene02480:CDS 60.0%
! TGCCGAGAGGTGTAGGTGGA+GGG - chr8.1:31133463-31133482 MS.gene02480:CDS 60.0%
GTAGGTGGAGGGAAAGGGGG+TGG - chr8.1:31133474-31133493 MS.gene02480:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 31133372 31133752 31133372 ID=MS.gene02480
chr8.1 mRNA 31133372 31133752 31133372 ID=MS.gene02480.t1;Parent=MS.gene02480
chr8.1 exon 31133372 31133752 31133372 ID=MS.gene02480.t1.exon1;Parent=MS.gene02480.t1
chr8.1 CDS 31133372 31133752 31133372 ID=cds.MS.gene02480.t1;Parent=MS.gene02480.t1
Gene Sequence

>MS.gene02480

ATGGATCTCGTTGAAGTAATGCGAGAGAAAAAGGGAATCAACGAGCTTCCGATTATGATGTTGATGGTTCCTGGAATGGGAATAAGGGGAATGCCGAGAGGTGTAGGTGGAGGGAAAGGGGGTGGTGATAGTGAAAAAGGGGGTGAGGTGAAGAAAGTTGAGGAGAAAACTGCGTTTGATGTGAAGCTTGATGGTTTTGATGCGGCGTCAAAGATTAAAGTTATTAAAGAAGTTAGGAGTTTTACTAGTTTGGGGTTGAAGGAAGCTAAGGATTTGGTGGAGAAATCGCCTACGCTTTTGAAGAAAGGTGTGACGAAAGAAGAGGCCGAGGGGATTATTGCTAAGTTAAAGGAGGTTGGTGCAAAAGCTTCTATGGAATAA

Protein sequence

>MS.gene02480.t1

MDLVEVMREKKGINELPIMMLMVPGMGIRGMPRGVGGGKGGGDSEKGGEVKKVEEKTAFDVKLDGFDAASKIKVIKEVRSFTSLGLKEAKDLVEKSPTLLKKGVTKEEAEGIIAKLKEVGAKASME