AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025762

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025762.t1 MTR_5g011540 97.652 511 12 0 1 511 1 511 0.0 1014
MS.gene025762.t1 MTR_6g043810 39.286 476 275 5 36 497 16 491 4.70e-104 321
MS.gene025762.t1 MTR_6g043810 39.286 476 275 5 36 497 26 501 4.86e-104 321
MS.gene025762.t1 MTR_5g067460 37.204 465 277 5 44 502 56 511 5.19e-100 312
MS.gene025762.t1 MTR_3g111020 37.166 487 285 6 13 486 15 493 7.31e-99 309
MS.gene025762.t1 MTR_5g010830 37.607 468 285 4 20 485 44 506 2.25e-98 307
MS.gene025762.t1 MTR_1g101500 37.778 495 291 7 25 506 39 529 1.55e-97 305
MS.gene025762.t1 MTR_0039s0080 35.657 502 313 7 13 510 9 504 5.22e-96 301
MS.gene025762.t1 MTR_6g088805 34.874 476 298 4 18 493 10 473 5.34e-89 281
MS.gene025762.t1 MTR_4g027920 32.829 463 308 2 41 501 154 615 6.56e-89 285
MS.gene025762.t1 MTR_8g102430 36.343 443 261 4 43 485 23 444 8.37e-88 277
MS.gene025762.t1 MTR_2g435930 31.884 483 302 3 28 489 4 480 1.14e-82 265
MS.gene025762.t1 MTR_6g081410 33.475 469 309 2 27 493 9 476 3.59e-74 243
MS.gene025762.t1 MTR_1g108810 33.105 438 288 2 43 478 34 468 4.93e-73 239
MS.gene025762.t1 MTR_6g081810 32.692 468 280 4 21 482 1 439 3.99e-69 228
MS.gene025762.t1 MTR_8g106670 32.071 449 298 5 38 483 7 451 8.52e-68 226
MS.gene025762.t1 MTR_6g081780 31.702 470 318 2 27 494 9 477 8.89e-68 226
MS.gene025762.t1 MTR_6g004220 32.826 460 296 5 30 483 11 463 2.13e-67 225
MS.gene025762.t1 MTR_6g004220 32.527 455 294 5 30 478 18 465 4.18e-65 219
MS.gene025762.t1 MTR_1g108840 30.818 477 322 4 9 483 10 480 8.26e-65 218
MS.gene025762.t1 MTR_6g081300 31.965 463 302 6 43 498 10 466 1.60e-64 217
MS.gene025762.t1 MTR_6g066380 32.719 434 289 2 51 482 35 467 1.13e-63 215
MS.gene025762.t1 MTR_2g078930 30.081 492 318 6 17 495 10 488 2.94e-63 214
MS.gene025762.t1 MTR_7g082810 29.814 483 311 7 30 501 7 472 4.69e-61 208
MS.gene025762.t1 MTR_2g078960 31.900 442 296 3 38 477 28 466 1.47e-59 204
MS.gene025762.t1 MTR_6g081400 30.425 447 303 5 41 483 13 455 3.39e-59 202
MS.gene025762.t1 MTR_2g079020 29.828 466 304 6 38 495 32 482 4.49e-58 201
MS.gene025762.t1 MTR_2g078970 30.435 460 309 4 38 495 26 476 1.65e-56 196
MS.gene025762.t1 MTR_6g081310 31.429 455 286 8 68 502 21 469 2.80e-56 195
MS.gene025762.t1 MTR_6g081390 29.047 451 304 7 41 483 10 452 9.02e-56 194
MS.gene025762.t1 MTR_2g022980 28.425 489 329 5 12 483 3 487 7.41e-55 192
MS.gene025762.t1 MTR_6g081240 30.227 440 298 6 43 478 10 444 4.29e-54 189
MS.gene025762.t1 MTR_2g088400 30.474 443 300 4 44 481 53 492 6.23e-53 187
MS.gene025762.t1 MTR_7g082800 30.923 401 261 4 102 497 1 390 9.08e-52 181
MS.gene025762.t1 MTR_4g048000 29.864 442 305 3 44 483 65 503 2.35e-51 183
MS.gene025762.t1 MTR_6g004220 33.065 372 236 5 30 395 18 382 5.92e-50 177
MS.gene025762.t1 MTR_1g109060 29.754 447 301 6 43 483 24 463 5.78e-49 176
MS.gene025762.t1 MTR_1g108990 30.180 444 303 4 43 483 25 464 7.91e-49 175
MS.gene025762.t1 MTR_2g088400 30.296 406 278 3 44 447 53 455 1.42e-48 174
MS.gene025762.t1 MTR_1g021740 27.430 463 328 4 25 483 15 473 8.67e-48 172
MS.gene025762.t1 MTR_1g109060 29.792 433 292 5 43 470 24 449 2.04e-47 171
MS.gene025762.t1 MTR_5g033100 26.844 488 333 7 12 483 15 494 1.03e-43 162
MS.gene025762.t1 MTR_8g069470 28.381 451 312 6 37 483 45 488 6.17e-42 157
MS.gene025762.t1 MTR_8g063590 26.292 445 324 3 40 482 50 492 6.69e-39 148
MS.gene025762.t1 MTR_5g090290 27.549 461 324 5 40 498 43 495 7.20e-39 149
MS.gene025762.t1 MTR_5g032720 26.852 432 313 2 54 484 47 476 1.44e-38 147
MS.gene025762.t1 MTR_6g004220 33.557 298 185 5 30 321 18 308 1.52e-38 144
MS.gene025762.t1 MTR_1g100180 26.439 469 335 5 38 502 39 501 4.72e-38 146
MS.gene025762.t1 MTR_7g087400 27.115 461 313 6 33 483 20 467 7.91e-37 142
MS.gene025762.t1 MTR_3g099720 25.826 484 351 4 8 489 8 485 2.44e-36 141
MS.gene025762.t1 MTR_6g047610 27.197 478 317 10 31 493 20 481 2.49e-36 141
MS.gene025762.t1 MTR_5g032750 26.566 463 334 3 26 484 16 476 3.97e-36 140
MS.gene025762.t1 MTR_7g087410 26.105 475 321 7 43 504 31 488 1.90e-34 135
MS.gene025762.t1 MTR_6g027200 28.513 491 324 10 1 483 3 474 6.47e-34 134
MS.gene025762.t1 MTR_6g027190 28.000 450 304 8 43 483 24 462 2.29e-33 132
MS.gene025762.t1 MTR_3g099700 27.202 511 339 11 9 501 7 502 4.38e-33 132
MS.gene025762.t1 MTR_6g047590 26.907 472 318 8 31 489 20 477 6.10e-33 131
MS.gene025762.t1 MTR_7g087430 25.899 417 284 5 102 509 1 401 1.97e-31 125
MS.gene025762.t1 MTR_6g047640 26.667 480 327 8 26 494 18 483 5.09e-31 125
MS.gene025762.t1 MTR_6g047640 26.042 480 311 10 26 494 18 464 1.13e-30 124
MS.gene025762.t1 MTR_7g087370 24.744 489 312 8 28 506 15 457 2.91e-30 123
MS.gene025762.t1 MTR_2g078990 28.296 311 212 4 187 495 4 305 3.95e-29 117
MS.gene025762.t1 MTR_5g087930 25.740 439 293 9 68 498 51 464 8.11e-29 119
MS.gene025762.t1 MTR_7g087410 25.610 410 281 6 105 504 4 399 1.09e-27 115
MS.gene025762.t1 MTR_2g079030 30.846 201 127 2 283 477 319 513 2.74e-24 106
MS.gene025762.t1 MTR_5g090280 23.474 426 277 10 102 493 1 411 8.61e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025762.t1 AT4G22790 58.873 479 195 2 16 493 3 480 0.0 564
MS.gene025762.t1 AT1G71870 37.895 475 278 5 35 492 15 489 2.28e-99 309
MS.gene025762.t1 AT4G29140 34.930 501 291 8 24 500 34 523 1.07e-95 300
MS.gene025762.t1 AT1G58340 39.520 458 266 3 43 495 57 508 8.68e-94 295
MS.gene025762.t1 AT5G19700 37.310 461 284 3 36 494 28 485 2.64e-93 293
MS.gene025762.t1 AT5G52050 31.703 511 332 6 11 510 1 505 2.25e-90 285
MS.gene025762.t1 AT2G38510 31.924 473 317 4 41 511 3 472 1.10e-83 268
MS.gene025762.t1 AT4G23030 36.952 479 291 5 30 502 15 488 1.20e-83 268
MS.gene025762.t1 AT5G49130 33.970 471 298 2 35 492 17 487 3.48e-83 267
MS.gene025762.t1 AT1G66760 30.608 477 324 3 18 491 2 474 2.08e-68 228
MS.gene025762.t1 AT2G04040 33.184 446 291 3 41 483 24 465 4.68e-68 226
MS.gene025762.t1 AT1G66760 30.603 464 315 3 18 478 2 461 9.68e-66 220
MS.gene025762.t1 AT5G52450 31.906 467 312 4 38 501 20 483 1.30e-65 220
MS.gene025762.t1 AT2G04080 30.454 463 315 3 24 483 7 465 2.54e-63 214
MS.gene025762.t1 AT2G34360 29.622 476 315 8 33 501 16 478 6.58e-63 213
MS.gene025762.t1 AT2G04070 30.670 463 314 3 24 483 7 465 1.52e-61 209
MS.gene025762.t1 AT1G64820 31.847 471 300 6 33 492 18 478 1.59e-61 210
MS.gene025762.t1 AT1G71140 30.131 458 312 4 29 483 12 464 2.35e-61 209
MS.gene025762.t1 AT2G04080 30.108 465 318 3 24 485 7 467 2.91e-61 208
MS.gene025762.t1 AT1G15150 29.116 498 332 5 12 502 3 486 3.12e-61 209
MS.gene025762.t1 AT1G15160 29.851 469 312 4 41 502 28 486 4.27e-61 208
MS.gene025762.t1 AT2G04100 30.562 445 304 2 41 483 27 468 6.30e-61 208
MS.gene025762.t1 AT1G66780 32.791 430 277 4 68 494 59 479 5.59e-60 205
MS.gene025762.t1 AT1G15170 31.991 447 289 4 44 483 34 472 1.98e-59 204
MS.gene025762.t1 AT1G73700 30.335 478 317 7 24 494 7 475 2.47e-59 203
MS.gene025762.t1 AT2G04050 31.780 472 306 5 21 483 1 465 7.76e-59 202
MS.gene025762.t1 AT2G04100 30.000 440 303 2 41 478 27 463 2.55e-58 201
MS.gene025762.t1 AT2G04090 29.130 460 320 3 27 483 12 468 8.07e-58 199
MS.gene025762.t1 AT1G15180 31.707 451 293 4 40 483 31 473 3.49e-55 192
MS.gene025762.t1 AT2G34360 28.361 476 279 9 33 501 16 436 4.60e-55 191
MS.gene025762.t1 AT3G23550 30.612 441 299 5 43 480 30 466 5.31e-52 184
MS.gene025762.t1 AT1G66760 30.051 396 270 3 18 410 2 393 1.81e-51 180
MS.gene025762.t1 AT1G61890 28.228 457 311 6 44 493 47 493 7.28e-51 181
MS.gene025762.t1 AT1G11670 28.188 447 306 5 44 483 50 488 7.64e-51 181
MS.gene025762.t1 AT1G61890 28.054 442 303 5 44 478 47 480 8.00e-49 176
MS.gene025762.t1 AT1G15170 30.726 358 233 4 133 483 4 353 4.16e-47 167
MS.gene025762.t1 AT1G47530 27.872 470 326 4 19 483 11 472 7.21e-47 170
MS.gene025762.t1 AT3G26590 27.975 479 324 8 33 501 28 495 5.89e-46 168
MS.gene025762.t1 AT3G21690 29.350 477 321 5 24 495 40 505 6.34e-46 168
MS.gene025762.t1 AT3G23560 29.186 442 308 3 38 477 33 471 7.56e-46 167
MS.gene025762.t1 AT1G61890 28.842 423 292 5 44 462 47 464 1.21e-45 166
MS.gene025762.t1 AT1G15150 28.912 377 251 4 133 502 4 370 4.57e-45 162
MS.gene025762.t1 AT4G21910 26.923 442 317 4 44 483 55 492 3.26e-44 163
MS.gene025762.t1 AT4G21910 26.923 442 317 4 44 483 55 492 3.26e-44 163
MS.gene025762.t1 AT1G12950 28.118 473 306 12 43 498 62 517 3.20e-43 160
MS.gene025762.t1 AT4G21910 27.088 443 315 5 44 483 57 494 6.64e-43 160
MS.gene025762.t1 AT4G21910 27.088 443 315 5 44 483 57 494 6.64e-43 160
MS.gene025762.t1 AT5G38030 27.292 469 328 5 24 483 18 482 1.02e-41 156
MS.gene025762.t1 AT3G26590 28.321 399 275 4 105 501 4 393 8.51e-41 151
MS.gene025762.t1 AT4G00350 24.440 491 341 5 11 483 46 524 1.08e-40 154
MS.gene025762.t1 AT4G21903 26.812 414 297 4 72 483 81 490 8.41e-40 151
MS.gene025762.t1 AT5G10420 28.540 452 305 6 54 499 48 487 8.80e-40 150
MS.gene025762.t1 AT5G44050 28.918 453 307 7 46 493 46 488 4.06e-39 149
MS.gene025762.t1 AT1G23300 26.793 474 323 8 51 510 51 514 2.00e-38 147
MS.gene025762.t1 AT4G21903 26.506 415 299 4 72 484 81 491 4.09e-38 146
MS.gene025762.t1 AT3G23560 30.058 346 237 3 38 381 33 375 1.34e-37 143
MS.gene025762.t1 AT4G21903 27.034 381 273 3 105 483 4 381 5.96e-37 141
MS.gene025762.t1 AT3G59030 24.658 511 366 8 1 501 1 502 9.28e-37 142
MS.gene025762.t1 AT5G65380 27.273 462 323 6 28 483 21 475 2.49e-36 141
MS.gene025762.t1 AT1G47530 27.604 384 266 3 105 483 4 380 1.46e-35 137
MS.gene025762.t1 AT4G25640 25.630 476 331 5 21 481 1 468 2.47e-34 135
MS.gene025762.t1 AT4G25640 25.630 476 331 5 21 481 1 468 3.08e-34 135
MS.gene025762.t1 AT1G33100 27.982 436 304 5 51 482 40 469 6.20e-34 134
MS.gene025762.t1 AT1G33110 28.959 442 292 6 51 482 43 472 3.61e-33 132
MS.gene025762.t1 AT1G33090 27.412 456 305 7 41 482 29 472 6.21e-33 131
MS.gene025762.t1 AT1G66760 27.893 337 236 3 145 478 1 333 2.29e-32 127
MS.gene025762.t1 AT4G25640 25.450 444 308 5 21 449 1 436 3.86e-31 126
MS.gene025762.t1 AT5G17700 25.758 462 312 9 43 492 33 475 4.25e-31 126
MS.gene025762.t1 AT5G17700 25.758 462 312 9 43 492 33 475 4.25e-31 126
MS.gene025762.t1 AT3G03620 26.190 462 323 7 28 481 21 472 5.68e-31 126
MS.gene025762.t1 AT3G03620 26.190 462 323 7 28 481 21 472 5.68e-31 126
MS.gene025762.t1 AT1G15180 29.779 272 176 4 133 397 4 267 8.50e-30 121
MS.gene025762.t1 AT1G33080 26.126 444 310 6 51 486 43 476 4.52e-29 120
MS.gene025762.t1 AT1G33080 25.909 440 308 6 51 482 43 472 2.44e-28 118
MS.gene025762.t1 AT3G59030 24.515 412 297 7 1 402 1 408 1.91e-23 103
MS.gene025762.t1 AT1G33110 30.055 366 226 8 51 402 43 392 2.24e-23 102
MS.gene025762.t1 AT5G17700 26.075 372 249 8 43 402 33 390 5.08e-21 95.5

Find 124 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGCTGCAAGGGGAATTT+GGG 0.166621 5.3:+5069500 MS.gene025762:CDS
CTCTTCTTTGGACTTCTTAC+TGG 0.194547 5.3:+5069750 MS.gene025762:CDS
GGCCTTGTTGGACTCTTCTT+TGG 0.195245 5.3:+5069738 MS.gene025762:CDS
CTTCCTACCATGTTTAGTTC+TGG 0.202562 5.3:+5068982 MS.gene025762:CDS
TTCACTTTCGCTAACGTTAC+TGG 0.209280 5.3:+5068661 MS.gene025762:CDS
TGGTGGCTGCAAGGGGAATT+TGG 0.224291 5.3:+5069499 MS.gene025762:CDS
TCTTATTGTTGTTGTTCTTC+TGG 0.235360 5.3:+5069095 MS.gene025762:CDS
AGCTTAGCCGGTGGCGCGCT+CGG 0.247230 5.3:+5068637 MS.gene025762:CDS
ATGTCATCAACATGCAAATT+AGG 0.269692 5.3:+5068400 MS.gene025762:CDS
GAAATTCTAGTCTTGCTTAC+TGG 0.273403 5.3:+5069264 MS.gene025762:CDS
CTCTAGAATTAAAACATAAA+TGG 0.277546 5.3:-5069118 None:intergenic
TTCCTACCATGTTTAGTTCT+GGG 0.312277 5.3:+5068983 MS.gene025762:CDS
TGAAGCCACATGAAAGTAAT+AGG 0.326554 5.3:-5068814 None:intergenic
CATGATAGGGGCACTATAAA+TGG 0.328539 5.3:+5069537 MS.gene025762:CDS
AAGCTTCGTCTTGGCCTTGT+TGG 0.336381 5.3:+5069726 MS.gene025762:CDS
CAAGAAATTTCCACTGTTGC+TGG 0.340485 5.3:+5068871 MS.gene025762:CDS
TTTGAGAGTAGTATGTTAAC+AGG 0.351355 5.3:-5069027 None:intergenic
CCATGAGCCTGTCCACAAAT+AGG 0.351874 5.3:-5068721 None:intergenic
CACAGGGAACTTCTTTAACT+TGG 0.357675 5.3:-5069866 None:intergenic
GACAATTGTATTGTTGCTAT+TGG 0.359414 5.3:+5068783 MS.gene025762:CDS
GCTTAGCCGGTGGCGCGCTC+GGG 0.363500 5.3:+5068638 MS.gene025762:CDS
AGAGTATTATGGATTGGATT+AGG 0.376619 5.3:+5069184 MS.gene025762:CDS
TGCTGTTGTGATTGCTGTCT+TGG 0.380543 5.3:-5068590 None:intergenic
CAGTGGAAATTTCTTGTTGT+TGG 0.381992 5.3:-5068864 None:intergenic
TTTCTCAGTGATGCTGTCAT+TGG 0.384106 5.3:+5069353 MS.gene025762:CDS
GAGGGGTTTCCATGGCTGTT+TGG 0.394339 5.3:+5069064 MS.gene025762:CDS
ATTAGGCTAATCAAGCTATC+TGG 0.398009 5.3:+5069201 MS.gene025762:CDS
GGCAACTCTTCAGCCATGAT+AGG 0.398212 5.3:+5069523 MS.gene025762:CDS
GTGGCTGCAAGGGGAATTTG+GGG 0.399284 5.3:+5069501 MS.gene025762:CDS
TCAGAGGAGCTAAGAGTTCA+AGG 0.400477 5.3:+5068523 MS.gene025762:CDS
ACTGGCTTCTCTGTCTTGAA+TGG 0.406137 5.3:+5068679 MS.gene025762:CDS
CGCAGGTTGCACCGGCAGCT+TGG 0.414906 5.3:+5069473 MS.gene025762:CDS
GCTTGGTGATGGTGGCTGCA+AGG 0.424849 5.3:+5069490 MS.gene025762:CDS
CGCAAACGCCAAGCAAGCAT+TGG 0.427319 5.3:+5069293 MS.gene025762:CDS
CGCCAAGCTCGTTTGAGACA+CGG 0.430905 5.3:-5069392 None:intergenic
CTTGGTGATGGTGGCTGCAA+GGG 0.432045 5.3:+5069491 MS.gene025762:CDS
TATTATTAGTATTCATTGCT+AGG 0.432752 5.3:+5069793 MS.gene025762:CDS
GCACGTGTGTCTCTAGGAAT+AGG 0.444169 5.3:+5069444 MS.gene025762:CDS
AGGGACAGCTCGACCTTGGT+TGG 0.445218 5.3:+5069638 MS.gene025762:CDS
TGGTACTTCTGCGTCATCAC+AGG 0.449230 5.3:-5069883 None:intergenic
TTTGCCTTCAAGCTTCGTCT+TGG 0.451622 5.3:+5069717 MS.gene025762:CDS
CATTTATAGTGCCCCTATCA+TGG 0.457544 5.3:-5069536 None:intergenic
ACCGGTACGCTCGGTCGGCT+TGG 0.459703 5.3:-5069419 None:intergenic
TAAAACTCCCAATGCTTGCT+TGG 0.462126 5.3:-5069301 None:intergenic
TCTAGAGAATCGAAAGGTAT+TGG 0.462933 5.3:+5069134 MS.gene025762:CDS
ACTTGCTAATATTTGTGCCT+TGG 0.463290 5.3:-5069838 None:intergenic
TCTGAAAGCAGCGTCTTCGC+TGG 0.467091 5.3:-5068505 None:intergenic
TTGGGTATGTATGCAAATCT+AGG 0.477841 5.3:+5069657 MS.gene025762:CDS
CGTGGAAAGAAGGAGGATGG+TGG 0.478669 5.3:+5069157 MS.gene025762:CDS
ATGGTGGGATCAGAGTATTA+TGG 0.479182 5.3:+5069173 MS.gene025762:CDS
CTAGGTGAGCTCAGCTTAGC+CGG 0.481845 5.3:+5068625 MS.gene025762:CDS
GCTAAATTCATCAACACCAT+TGG 0.482192 5.3:-5068559 None:intergenic
GCAACTCTTCAGCCATGATA+GGG 0.483823 5.3:+5069524 MS.gene025762:CDS
AGTGACATAACAAACAAATC+AGG 0.486224 5.3:-5068916 None:intergenic
GAATGGTCTCTGTGGTGCCA+TGG 0.486890 5.3:+5068696 MS.gene025762:CDS
TTGTTGTCTCAACACATGTA+TGG 0.488430 5.3:+5069227 MS.gene025762:CDS
GGGACAGCTCGACCTTGGTT+GGG 0.495004 5.3:+5069639 MS.gene025762:CDS
GGGATCAGAGTATTATGGAT+TGG 0.495416 5.3:+5069178 MS.gene025762:CDS
TCCTACCATGTTTAGTTCTG+GGG 0.499436 5.3:+5068984 MS.gene025762:CDS
ACCCGTGTCTCAAACGAGCT+TGG 0.500843 5.3:+5069390 MS.gene025762:CDS
CCTATTTGTGGACAGGCTCA+TGG 0.501376 5.3:+5068721 MS.gene025762:CDS
TATTCATTGCTAGGATAAAG+TGG 0.503924 5.3:+5069802 MS.gene025762:CDS
CGGTCAGCACGTGTGTCTCT+AGG 0.507994 5.3:+5069438 MS.gene025762:CDS
AAGGCCAAGACGAAGCTTGA+AGG 0.508427 5.3:-5069721 None:intergenic
GGGTCTAAGAGGGGTTTCCA+TGG 0.510224 5.3:+5069056 MS.gene025762:CDS
GCCAAGCTCGTTTGAGACAC+GGG 0.510977 5.3:-5069391 None:intergenic
AGTGAACCCGAGCGCGCCAC+CGG 0.515331 5.3:-5068644 None:intergenic
AAGGTATTGGCGTGGAAAGA+AGG 0.518806 5.3:+5069147 MS.gene025762:CDS
GCAAACGCCAAGCAAGCATT+GGG 0.519010 5.3:+5069294 MS.gene025762:CDS
AGCCACCATCACCAAGCTGC+CGG 0.520002 5.3:-5069484 None:intergenic
CGGGTCTGTAATGGTGGAGG+AGG 0.520121 5.3:-5068466 None:intergenic
CATGGAACCTATTTGTGGAC+AGG 0.523239 5.3:+5068714 MS.gene025762:CDS
TTGCACCGGCAGCTTGGTGA+TGG 0.528564 5.3:+5069479 MS.gene025762:CDS
GTGGAGGAGGTGACATTGGT+TGG 0.531112 5.3:-5068453 None:intergenic
TTCGAGGGACAGCTCGACCT+TGG 0.533695 5.3:+5069634 MS.gene025762:CDS
CACCGGCAGCTTGGTGATGG+TGG 0.540745 5.3:+5069482 MS.gene025762:CDS
AGCGAAGACGCTGCTTTCAG+AGG 0.548362 5.3:+5068507 MS.gene025762:CDS
GTTTGTGGAGGCATAGTTCG+AGG 0.549958 5.3:+5069618 MS.gene025762:CDS
ATGCCTCCACAAACTGCCAA+TGG 0.550253 5.3:-5069609 None:intergenic
ATCAGTTATCCAAACAGCCA+TGG 0.552397 5.3:-5069073 None:intergenic
TGGCGTGGAAAGAAGGAGGA+TGG 0.552982 5.3:+5069154 MS.gene025762:CDS
CACGTGCTGACCGGTACGCT+CGG 0.555036 5.3:-5069428 None:intergenic
CTAGAGACACACGTGCTGAC+CGG 0.558867 5.3:-5069437 None:intergenic
AATGTTGTTGATGGCTCTAG+TGG 0.559949 5.3:+5069572 MS.gene025762:CDS
TTTCGGGTCTGTAATGGTGG+AGG 0.563765 5.3:-5068469 None:intergenic
GTATGTATGCAAATCTAGGC+GGG 0.568375 5.3:+5069661 MS.gene025762:CDS
CTTGCTTGGCGTTTGCGAGG+TGG 0.568986 5.3:-5069287 None:intergenic
GGTGCCATGGAACCTATTTG+TGG 0.570180 5.3:+5068709 MS.gene025762:CDS
GTGGAAAGAAGGAGGATGGT+GGG 0.570875 5.3:+5069158 MS.gene025762:CDS
CTGTCCACAAATAGGTTCCA+TGG 0.574641 5.3:-5068713 None:intergenic
CAATTCCTATTACTTTCATG+TGG 0.579908 5.3:+5068809 MS.gene025762:CDS
ACCAAGCCGACCGAGCGTAC+CGG 0.581822 5.3:+5069418 MS.gene025762:CDS
GGAAACCCCTCTTAGACCCA+TGG 0.585351 5.3:-5069052 None:intergenic
TTTCCATTGGCAGTTTGTGG+AGG 0.586502 5.3:+5069606 MS.gene025762:CDS
TGGCTGCAAGGGGAATTTGG+GGG 0.591531 5.3:+5069502 MS.gene025762:CDS
TGGTGTTGATGAATTTAGCT+TGG 0.591762 5.3:+5068563 MS.gene025762:CDS
AACATAAGTACCAGCAACAG+TGG 0.598946 5.3:-5068881 None:intergenic
TCATGAGAAGCGTCTTGTGA+AGG 0.602612 5.3:-5068762 None:intergenic
GTGGGTGGAAGAAGCTGCCA+AGG 0.602858 5.3:+5069821 MS.gene025762:CDS
TTGGTGATGGTGGCTGCAAG+GGG 0.610161 5.3:+5069492 MS.gene025762:CDS
ATGCTTGCTTGGCGTTTGCG+AGG 0.612886 5.3:-5069290 None:intergenic
TGCTGACCGGTACGCTCGGT+CGG 0.613523 5.3:-5069424 None:intergenic
TTTAATTCTAGAGAATCGAA+AGG 0.625448 5.3:+5069128 MS.gene025762:CDS
GGTACTTCTGCGTCATCACA+GGG 0.629425 5.3:-5069882 None:intergenic
GTATTGGCGTGGAAAGAAGG+AGG 0.632332 5.3:+5069150 MS.gene025762:CDS
ATTCATTGCTAGGATAAAGT+GGG 0.633439 5.3:+5069803 MS.gene025762:CDS
CAACTCTTCAGCCATGATAG+GGG 0.633765 5.3:+5069525 MS.gene025762:CDS
AATGGTGGAGGAGGTGACAT+TGG 0.637951 5.3:-5068457 None:intergenic
GGTGAGCTCAGCTTAGCCGG+TGG 0.638224 5.3:+5068628 MS.gene025762:CDS
TTTGTGGAGGCATAGTTCGA+GGG 0.638241 5.3:+5069619 MS.gene025762:CDS
GACACGGGTGGAAACACAAG+TGG 0.646609 5.3:-5069376 None:intergenic
GTCCAAAGAAGAGTCCAACA+AGG 0.652628 5.3:-5069740 None:intergenic
CATTGCTAGGATAAAGTGGG+TGG 0.653404 5.3:+5069806 MS.gene025762:CDS
TCTGTCTTGAATGGTCTCTG+TGG 0.655881 5.3:+5068688 MS.gene025762:CDS
AGAATCGAAAGGTATTGGCG+TGG 0.660271 5.3:+5069139 MS.gene025762:CDS
TCAACACATGTATGGAGTGG+TGG 0.666907 5.3:+5069235 MS.gene025762:CDS
TGCTACCCCAGAACTAAACA+TGG 0.676669 5.3:-5068989 None:intergenic
ATAGTCAAAGTTCAACACAA+TGG 0.680940 5.3:-5069325 None:intergenic
ACCCCAGAACTAAACATGGT+AGG 0.687373 5.3:-5068985 None:intergenic
GTCTCAACACATGTATGGAG+TGG 0.690627 5.3:+5069232 MS.gene025762:CDS
AAGCTCGTTTGAGACACGGG+TGG 0.702539 5.3:-5069388 None:intergenic
GGTATGTATGCAAATCTAGG+CGG 0.704766 5.3:+5069660 MS.gene025762:CDS
TGACGCAGAAGTACCAACTG+AGG 0.735542 5.3:+5069890 MS.gene025762:CDS
TTTGCATACATACCCAACCA+AGG 0.735927 5.3:-5069651 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTAGTATTCATTGCT+AGG + chr5.3:5069793-5069812 MS.gene025762:CDS 20.0%
!!! CTCTAGAATTAAAACATAAA+TGG - chr5.3:5069121-5069140 None:intergenic 20.0%
!!! GACAAAATTTTGATTCATTT+TGG + chr5.3:5068841-5068860 MS.gene025762:CDS 20.0%
! AAAAAAGACAATGTTGTTGA+TGG + chr5.3:5069563-5069582 MS.gene025762:CDS 25.0%
!! TTTAATTCTAGAGAATCGAA+AGG + chr5.3:5069128-5069147 MS.gene025762:CDS 25.0%
AGTGACATAACAAACAAATC+AGG - chr5.3:5068919-5068938 None:intergenic 30.0%
ATACTACTCTCAAAAACCAT+GGG + chr5.3:5069036-5069055 MS.gene025762:CDS 30.0%
ATAGTCAAAGTTCAACACAA+TGG - chr5.3:5069328-5069347 None:intergenic 30.0%
ATGTCATCAACATGCAAATT+AGG + chr5.3:5068400-5068419 MS.gene025762:CDS 30.0%
ATTCATTGCTAGGATAAAGT+GGG + chr5.3:5069803-5069822 MS.gene025762:CDS 30.0%
CAATTCCTATTACTTTCATG+TGG + chr5.3:5068809-5068828 MS.gene025762:CDS 30.0%
GACAATTGTATTGTTGCTAT+TGG + chr5.3:5068783-5068802 MS.gene025762:CDS 30.0%
TATTCATTGCTAGGATAAAG+TGG + chr5.3:5069802-5069821 MS.gene025762:CDS 30.0%
TTTGAGAGTAGTATGTTAAC+AGG - chr5.3:5069030-5069049 None:intergenic 30.0%
! TCTTATTGTTGTTGTTCTTC+TGG + chr5.3:5069095-5069114 MS.gene025762:CDS 30.0%
!! AGAGTATTATGGATTGGATT+AGG + chr5.3:5069184-5069203 MS.gene025762:CDS 30.0%
!! GGAGTTGTTTAATTTTCCAT+TGG + chr5.3:5069593-5069612 MS.gene025762:CDS 30.0%
!!! ACATTGGTTGGTTAGTTTTA+TGG - chr5.3:5068444-5068463 None:intergenic 30.0%
!!! TAGTTTTATGGTTTGAGCTA+AGG - chr5.3:5068432-5068451 None:intergenic 30.0%
ATTAGGCTAATCAAGCTATC+TGG + chr5.3:5069201-5069220 MS.gene025762:CDS 35.0%
CATACTACTCTCAAAAACCA+TGG + chr5.3:5069035-5069054 MS.gene025762:CDS 35.0%
GCTAAATTCATCAACACCAT+TGG - chr5.3:5068562-5068581 None:intergenic 35.0%
TGAAGCCACATGAAAGTAAT+AGG - chr5.3:5068817-5068836 None:intergenic 35.0%
TTGGGTATGTATGCAAATCT+AGG + chr5.3:5069657-5069676 MS.gene025762:CDS 35.0%
TTGTTGTCTCAACACATGTA+TGG + chr5.3:5069227-5069246 MS.gene025762:CDS 35.0%
! AATTTTCCATTGGCAGTTTG+TGG + chr5.3:5069603-5069622 MS.gene025762:CDS 35.0%
! ACTTGCTAATATTTGTGCCT+TGG - chr5.3:5069841-5069860 None:intergenic 35.0%
! AGAGCTCAAGTAAGCTTTTA+AGG - chr5.3:5068950-5068969 None:intergenic 35.0%
! AGCAATCACAACAGCATTTT+TGG + chr5.3:5068597-5068616 MS.gene025762:CDS 35.0%
! CAGTGGAAATTTCTTGTTGT+TGG - chr5.3:5068867-5068886 None:intergenic 35.0%
! GAGCTCAAGTAAGCTTTTAA+GGG - chr5.3:5068949-5068968 None:intergenic 35.0%
! GCAATCACAACAGCATTTTT+GGG + chr5.3:5068598-5068617 MS.gene025762:CDS 35.0%
! TCTAGAGAATCGAAAGGTAT+TGG + chr5.3:5069134-5069153 MS.gene025762:CDS 35.0%
!! GAAATTCTAGTCTTGCTTAC+TGG + chr5.3:5069264-5069283 MS.gene025762:CDS 35.0%
!! TGGTGTTGATGAATTTAGCT+TGG + chr5.3:5068563-5068582 MS.gene025762:CDS 35.0%
!! TTCCTACCATGTTTAGTTCT+GGG + chr5.3:5068983-5069002 MS.gene025762:CDS 35.0%
!!! AGGAAGATTAGCTTTTCCAA+TGG + chr5.3:5068543-5068562 MS.gene025762:CDS 35.0%
AAAAACCATGGGTCTAAGAG+GGG + chr5.3:5069047-5069066 MS.gene025762:CDS 40.0%
AACATAAGTACCAGCAACAG+TGG - chr5.3:5068884-5068903 None:intergenic 40.0%
AAGGCAAAAACAACACCAAG+GGG - chr5.3:5069705-5069724 None:intergenic 40.0%
ATCAGTTATCCAAACAGCCA+TGG - chr5.3:5069076-5069095 None:intergenic 40.0%
CAAAAACCATGGGTCTAAGA+GGG + chr5.3:5069046-5069065 MS.gene025762:CDS 40.0%
CAAGAAATTTCCACTGTTGC+TGG + chr5.3:5068871-5068890 MS.gene025762:CDS 40.0%
CACAGGGAACTTCTTTAACT+TGG - chr5.3:5069869-5069888 None:intergenic 40.0%
CATTTATAGTGCCCCTATCA+TGG - chr5.3:5069539-5069558 None:intergenic 40.0%
CTCTTCTTTGGACTTCTTAC+TGG + chr5.3:5069750-5069769 MS.gene025762:CDS 40.0%
GAAGGCAAAAACAACACCAA+GGG - chr5.3:5069706-5069725 None:intergenic 40.0%
GGTATGTATGCAAATCTAGG+CGG + chr5.3:5069660-5069679 MS.gene025762:CDS 40.0%
GTATGTATGCAAATCTAGGC+GGG + chr5.3:5069661-5069680 MS.gene025762:CDS 40.0%
TAAAACTCCCAATGCTTGCT+TGG - chr5.3:5069304-5069323 None:intergenic 40.0%
TCAAAAACCATGGGTCTAAG+AGG + chr5.3:5069045-5069064 MS.gene025762:CDS 40.0%
TGAAGGCAAAAACAACACCA+AGG - chr5.3:5069707-5069726 None:intergenic 40.0%
TTCACTTTCGCTAACGTTAC+TGG + chr5.3:5068661-5068680 MS.gene025762:CDS 40.0%
TTTCTCAGTGATGCTGTCAT+TGG + chr5.3:5069353-5069372 MS.gene025762:CDS 40.0%
TTTGCATACATACCCAACCA+AGG - chr5.3:5069654-5069673 None:intergenic 40.0%
! ATGGTGGGATCAGAGTATTA+TGG + chr5.3:5069173-5069192 MS.gene025762:CDS 40.0%
! CTGGATTTGCGTCACATTTT+CGG - chr5.3:5068489-5068508 None:intergenic 40.0%
! CTTCCTACCATGTTTAGTTC+TGG + chr5.3:5068982-5069001 MS.gene025762:CDS 40.0%
! GGGATCAGAGTATTATGGAT+TGG + chr5.3:5069178-5069197 MS.gene025762:CDS 40.0%
! TGGATTTGCGTCACATTTTC+GGG - chr5.3:5068488-5068507 None:intergenic 40.0%
!! AATGTTGTTGATGGCTCTAG+TGG + chr5.3:5069572-5069591 MS.gene025762:CDS 40.0%
!! CATGATAGGGGCACTATAAA+TGG + chr5.3:5069537-5069556 MS.gene025762:CDS 40.0%
!! CTAGGAATAGGTTTTATCGC+AGG + chr5.3:5069456-5069475 MS.gene025762:CDS 40.0%
!! TCCTACCATGTTTAGTTCTG+GGG + chr5.3:5068984-5069003 MS.gene025762:CDS 40.0%
!!! TCACATTTTCGGGTCTGTAA+TGG - chr5.3:5068478-5068497 None:intergenic 40.0%
!!! TCTAGGCGGGTTTTATTTTC+TGG + chr5.3:5069674-5069693 MS.gene025762:CDS 40.0%
ACCCCAGAACTAAACATGGT+AGG - chr5.3:5068988-5069007 None:intergenic 45.0%
CAACTCTTCAGCCATGATAG+GGG + chr5.3:5069525-5069544 MS.gene025762:CDS 45.0%
CATGGAACCTATTTGTGGAC+AGG + chr5.3:5068714-5068733 MS.gene025762:CDS 45.0%
CATTGCTAGGATAAAGTGGG+TGG + chr5.3:5069806-5069825 MS.gene025762:CDS 45.0%
CTGTCCACAAATAGGTTCCA+TGG - chr5.3:5068716-5068735 None:intergenic 45.0%
GCAACTCTTCAGCCATGATA+GGG + chr5.3:5069524-5069543 MS.gene025762:CDS 45.0%
GTCCAAAGAAGAGTCCAACA+AGG - chr5.3:5069743-5069762 None:intergenic 45.0%
GTCTCAACACATGTATGGAG+TGG + chr5.3:5069232-5069251 MS.gene025762:CDS 45.0%
TCAACACATGTATGGAGTGG+TGG + chr5.3:5069235-5069254 MS.gene025762:CDS 45.0%
TCAGAGGAGCTAAGAGTTCA+AGG + chr5.3:5068523-5068542 MS.gene025762:CDS 45.0%
TCTGTCTTGAATGGTCTCTG+TGG + chr5.3:5068688-5068707 MS.gene025762:CDS 45.0%
TGCTACCCCAGAACTAAACA+TGG - chr5.3:5068992-5069011 None:intergenic 45.0%
TGCTGTTGTGATTGCTGTCT+TGG - chr5.3:5068593-5068612 None:intergenic 45.0%
TTTCCATTGGCAGTTTGTGG+AGG + chr5.3:5069606-5069625 MS.gene025762:CDS 45.0%
TTTGCCTTCAAGCTTCGTCT+TGG + chr5.3:5069717-5069736 MS.gene025762:CDS 45.0%
! ACTGGCTTCTCTGTCTTGAA+TGG + chr5.3:5068679-5068698 MS.gene025762:CDS 45.0%
! TCATTTTCACGAGCCTCAGT+TGG - chr5.3:5069906-5069925 None:intergenic 45.0%
! TTTGTGGAGGCATAGTTCGA+GGG + chr5.3:5069619-5069638 MS.gene025762:CDS 45.0%
!! AAGGTATTGGCGTGGAAAGA+AGG + chr5.3:5069147-5069166 MS.gene025762:CDS 45.0%
!! AGAATCGAAAGGTATTGGCG+TGG + chr5.3:5069139-5069158 MS.gene025762:CDS 45.0%
!! TCATGAGAAGCGTCTTGTGA+AGG - chr5.3:5068765-5068784 None:intergenic 45.0%
!!! ACAGCATTTTTGGGTCGACT+AGG + chr5.3:5068607-5068626 MS.gene025762:CDS 45.0%
!!! CATTTTCGGGTCTGTAATGG+TGG - chr5.3:5068475-5068494 None:intergenic 45.0%
AAGGCCAAGACGAAGCTTGA+AGG - chr5.3:5069724-5069743 None:intergenic 50.0%
ATGCCTCCACAAACTGCCAA+TGG - chr5.3:5069612-5069631 None:intergenic 50.0%
CCATGAGCCTGTCCACAAAT+AGG - chr5.3:5068724-5068743 None:intergenic 50.0%
CCTATTTGTGGACAGGCTCA+TGG + chr5.3:5068721-5068740 MS.gene025762:CDS 50.0%
GCAAACGCCAAGCAAGCATT+GGG + chr5.3:5069294-5069313 MS.gene025762:CDS 50.0%
GGCAACTCTTCAGCCATGAT+AGG + chr5.3:5069523-5069542 MS.gene025762:CDS 50.0%
GGTACTTCTGCGTCATCACA+GGG - chr5.3:5069885-5069904 None:intergenic 50.0%
GTGGAAAGAAGGAGGATGGT+GGG + chr5.3:5069158-5069177 MS.gene025762:CDS 50.0%
TGGTACTTCTGCGTCATCAC+AGG - chr5.3:5069886-5069905 None:intergenic 50.0%
! AATGGTGGAGGAGGTGACAT+TGG - chr5.3:5068460-5068479 None:intergenic 50.0%
! GCACGTGTGTCTCTAGGAAT+AGG + chr5.3:5069444-5069463 MS.gene025762:CDS 50.0%
! GGTGCCATGGAACCTATTTG+TGG + chr5.3:5068709-5068728 MS.gene025762:CDS 50.0%
! GTATTGGCGTGGAAAGAAGG+AGG + chr5.3:5069150-5069169 MS.gene025762:CDS 50.0%
! GTTTGTGGAGGCATAGTTCG+AGG + chr5.3:5069618-5069637 MS.gene025762:CDS 50.0%
! TTTCGGGTCTGTAATGGTGG+AGG - chr5.3:5068472-5068491 None:intergenic 50.0%
!! AAGCTTCGTCTTGGCCTTGT+TGG + chr5.3:5069726-5069745 MS.gene025762:CDS 50.0%
!! GGCCTTGTTGGACTCTTCTT+TGG + chr5.3:5069738-5069757 MS.gene025762:CDS 50.0%
!! GGTTTTATCGCAGGTTGCAC+CGG + chr5.3:5069465-5069484 MS.gene025762:CDS 50.0%
!! TGACGCAGAAGTACCAACTG+AGG + chr5.3:5069890-5069909 MS.gene025762:CDS 50.0%
!!! TATTTTCTGGCTCTGCCCCT+TGG + chr5.3:5069687-5069706 MS.gene025762:CDS 50.0%
ACCCGTGTCTCAAACGAGCT+TGG + chr5.3:5069390-5069409 MS.gene025762:CDS 55.0%
ATGCTTGCTTGGCGTTTGCG+AGG - chr5.3:5069293-5069312 None:intergenic 55.0%
CGCAAACGCCAAGCAAGCAT+TGG + chr5.3:5069293-5069312 MS.gene025762:CDS 55.0%
CGTGGAAAGAAGGAGGATGG+TGG + chr5.3:5069157-5069176 MS.gene025762:CDS 55.0%
CTAGGTGAGCTCAGCTTAGC+CGG + chr5.3:5068625-5068644 MS.gene025762:CDS 55.0%
GACACGGGTGGAAACACAAG+TGG - chr5.3:5069379-5069398 None:intergenic 55.0%
GAGGGGTTTCCATGGCTGTT+TGG + chr5.3:5069064-5069083 MS.gene025762:CDS 55.0%
GGAAACCCCTCTTAGACCCA+TGG - chr5.3:5069055-5069074 None:intergenic 55.0%
GGGTCTAAGAGGGGTTTCCA+TGG + chr5.3:5069056-5069075 MS.gene025762:CDS 55.0%
GGTGGCTGCAAGGGGAATTT+GGG + chr5.3:5069500-5069519 MS.gene025762:CDS 55.0%
GTGGAGGAGGTGACATTGGT+TGG - chr5.3:5068456-5068475 None:intergenic 55.0%
GTGGCTGCAAGGGGAATTTG+GGG + chr5.3:5069501-5069520 MS.gene025762:CDS 55.0%
TCTGAAAGCAGCGTCTTCGC+TGG - chr5.3:5068508-5068527 None:intergenic 55.0%
TGGCGTGGAAAGAAGGAGGA+TGG + chr5.3:5069154-5069173 MS.gene025762:CDS 55.0%
TGGCTGCAAGGGGAATTTGG+GGG + chr5.3:5069502-5069521 MS.gene025762:CDS 55.0%
TGGTGGCTGCAAGGGGAATT+TGG + chr5.3:5069499-5069518 MS.gene025762:CDS 55.0%
! AAGCTCGTTTGAGACACGGG+TGG - chr5.3:5069391-5069410 None:intergenic 55.0%
! AGCGAAGACGCTGCTTTCAG+AGG + chr5.3:5068507-5068526 MS.gene025762:CDS 55.0%
! CGCCAAGCTCGTTTGAGACA+CGG - chr5.3:5069395-5069414 None:intergenic 55.0%
! CTAGAGACACACGTGCTGAC+CGG - chr5.3:5069440-5069459 None:intergenic 55.0%
! GAATGGTCTCTGTGGTGCCA+TGG + chr5.3:5068696-5068715 MS.gene025762:CDS 55.0%
! GCCAAGCTCGTTTGAGACAC+GGG - chr5.3:5069394-5069413 None:intergenic 55.0%
!! CTTGGTGATGGTGGCTGCAA+GGG + chr5.3:5069491-5069510 MS.gene025762:CDS 55.0%
!! TTGGTGATGGTGGCTGCAAG+GGG + chr5.3:5069492-5069511 MS.gene025762:CDS 55.0%
AGCCACCATCACCAAGCTGC+CGG - chr5.3:5069487-5069506 None:intergenic 60.0%
AGGGACAGCTCGACCTTGGT+TGG + chr5.3:5069638-5069657 MS.gene025762:CDS 60.0%
CGGTCAGCACGTGTGTCTCT+AGG + chr5.3:5069438-5069457 MS.gene025762:CDS 60.0%
CTTGCTTGGCGTTTGCGAGG+TGG - chr5.3:5069290-5069309 None:intergenic 60.0%
GGGACAGCTCGACCTTGGTT+GGG + chr5.3:5069639-5069658 MS.gene025762:CDS 60.0%
GTGGGTGGAAGAAGCTGCCA+AGG + chr5.3:5069821-5069840 MS.gene025762:CDS 60.0%
TTCGAGGGACAGCTCGACCT+TGG + chr5.3:5069634-5069653 MS.gene025762:CDS 60.0%
! CGGGTCTGTAATGGTGGAGG+AGG - chr5.3:5068469-5068488 None:intergenic 60.0%
! TTGCACCGGCAGCTTGGTGA+TGG + chr5.3:5069479-5069498 MS.gene025762:CDS 60.0%
!! GCTTGGTGATGGTGGCTGCA+AGG + chr5.3:5069490-5069509 MS.gene025762:CDS 60.0%
ACCAAGCCGACCGAGCGTAC+CGG + chr5.3:5069418-5069437 MS.gene025762:CDS 65.0%
GGTGAGCTCAGCTTAGCCGG+TGG + chr5.3:5068628-5068647 MS.gene025762:CDS 65.0%
TGCTGACCGGTACGCTCGGT+CGG - chr5.3:5069427-5069446 None:intergenic 65.0%
! CACGTGCTGACCGGTACGCT+CGG - chr5.3:5069431-5069450 None:intergenic 65.0%
!! CACCGGCAGCTTGGTGATGG+TGG + chr5.3:5069482-5069501 MS.gene025762:CDS 65.0%
ACCGGTACGCTCGGTCGGCT+TGG - chr5.3:5069422-5069441 None:intergenic 70.0%
AGCTTAGCCGGTGGCGCGCT+CGG + chr5.3:5068637-5068656 MS.gene025762:CDS 70.0%
AGTGAACCCGAGCGCGCCAC+CGG - chr5.3:5068647-5068666 None:intergenic 70.0%
! CGCAGGTTGCACCGGCAGCT+TGG + chr5.3:5069473-5069492 MS.gene025762:CDS 70.0%
GCTTAGCCGGTGGCGCGCTC+GGG + chr5.3:5068638-5068657 MS.gene025762:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 5068400 5069935 5068400 ID=MS.gene025762
chr5.3 mRNA 5068400 5069935 5068400 ID=MS.gene025762.t1;Parent=MS.gene025762
chr5.3 exon 5068400 5069935 5068400 ID=MS.gene025762.t1.exon1;Parent=MS.gene025762.t1
chr5.3 CDS 5068400 5069935 5068400 ID=cds.MS.gene025762.t1;Parent=MS.gene025762.t1
Gene Sequence

>MS.gene025762

ATGTCATCAACATGCAAATTAGGAGAAAACCTTAGCTCAAACCATAAAACTAACCAACCAATGTCACCTCCTCCACCATTACAGACCCGAAAATGTGACGCAAATCCAGCGAAGACGCTGCTTTCAGAGGAGCTAAGAGTTCAAGGAAGATTAGCTTTTCCAATGGTGTTGATGAATTTAGCTTGGTTTGCCAAGACAGCAATCACAACAGCATTTTTGGGTCGACTAGGTGAGCTCAGCTTAGCCGGTGGCGCGCTCGGGTTCACTTTCGCTAACGTTACTGGCTTCTCTGTCTTGAATGGTCTCTGTGGTGCCATGGAACCTATTTGTGGACAGGCTCATGGAGCTAAAAACGTGAGACTCCTTCACAAGACGCTTCTCATGACAATTGTATTGTTGCTATTGGTAACAATTCCTATTACTTTCATGTGGCTTCATATTGACAAAATTTTGATTCATTTTGGCCAACAACAAGAAATTTCCACTGTTGCTGGTACTTATGTTTACTATCTCATACCTGATTTGTTTGTTATGTCACTCTTGTGTCCCTTAAAAGCTTACTTGAGCTCTCAAAGCATCACTCTTCCTACCATGTTTAGTTCTGGGGTAGCACTTGCTTTTCATGTTCCTGTTAACATACTACTCTCAAAAACCATGGGTCTAAGAGGGGTTTCCATGGCTGTTTGGATAACTGATCTTATTGTTGTTGTTCTTCTGGCCATTTATGTTTTAATTCTAGAGAATCGAAAGGTATTGGCGTGGAAAGAAGGAGGATGGTGGGATCAGAGTATTATGGATTGGATTAGGCTAATCAAGCTATCTGGATCTTGTTGTCTCAACACATGTATGGAGTGGTGGTGCTATGAAATTCTAGTCTTGCTTACTGGCCACCTCGCAAACGCCAAGCAAGCATTGGGAGTTTTAGCCATTGTGTTGAACTTTGACTATTTACTTTTCTCAGTGATGCTGTCATTGGCCACTTGTGTTTCCACCCGTGTCTCAAACGAGCTTGGCGCAAACCAAGCCGACCGAGCGTACCGGTCAGCACGTGTGTCTCTAGGAATAGGTTTTATCGCAGGTTGCACCGGCAGCTTGGTGATGGTGGCTGCAAGGGGAATTTGGGGGCAACTCTTCAGCCATGATAGGGGCACTATAAATGGAGTAAAAAAGACAATGTTGTTGATGGCTCTAGTGGAGTTGTTTAATTTTCCATTGGCAGTTTGTGGAGGCATAGTTCGAGGGACAGCTCGACCTTGGTTGGGTATGTATGCAAATCTAGGCGGGTTTTATTTTCTGGCTCTGCCCCTTGGTGTTGTTTTTGCCTTCAAGCTTCGTCTTGGCCTTGTTGGACTCTTCTTTGGACTTCTTACTGGTATTGTTGTTTGCTTGTCATTATTATTAGTATTCATTGCTAGGATAAAGTGGGTGGAAGAAGCTGCCAAGGCACAAATATTAGCAAGTAACGACCAAGTTAAAGAAGTTCCCTGTGATGACGCAGAAGTACCAACTGAGGCTCGTGAAAATGACAAAGTGTAA
Protein sequence

>MS.gene025762.t1

MSSTCKLGENLSSNHKTNQPMSPPPPLQTRKCDANPAKTLLSEELRVQGRLAFPMVLMNLAWFAKTAITTAFLGRLGELSLAGGALGFTFANVTGFSVLNGLCGAMEPICGQAHGAKNVRLLHKTLLMTIVLLLLVTIPITFMWLHIDKILIHFGQQQEISTVAGTYVYYLIPDLFVMSLLCPLKAYLSSQSITLPTMFSSGVALAFHVPVNILLSKTMGLRGVSMAVWITDLIVVVLLAIYVLILENRKVLAWKEGGWWDQSIMDWIRLIKLSGSCCLNTCMEWWCYEILVLLTGHLANAKQALGVLAIVLNFDYLLFSVMLSLATCVSTRVSNELGANQADRAYRSARVSLGIGFIAGCTGSLVMVAARGIWGQLFSHDRGTINGVKKTMLLMALVELFNFPLAVCGGIVRGTARPWLGMYANLGGFYFLALPLGVVFAFKLRLGLVGLFFGLLTGIVVCLSLLLVFIARIKWVEEAAKAQILASNDQVKEVPCDDAEVPTEARENDKV