AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026953

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026953.t1 MTR_6g007170 92.201 359 28 0 345 703 28 386 0.0 687
MS.gene026953.t1 MTR_6g007170 94.638 373 18 1 1 371 1 373 0.0 681
MS.gene026953.t1 MTR_6g007175 92.938 354 6 1 350 703 2 336 0.0 672
MS.gene026953.t1 MTR_6g007175 82.143 336 39 2 33 366 2 318 0.0 549
MS.gene026953.t1 MTR_4g005880 31.004 229 152 4 122 349 152 375 1.42e-24 109
MS.gene026953.t1 MTR_4g005880 28.455 246 172 3 439 684 152 393 8.99e-24 107
MS.gene026953.t1 MTR_4g013090 27.799 259 170 5 431 684 60 306 4.36e-23 100
MS.gene026953.t1 MTR_4g013090 26.749 243 163 4 113 351 59 290 5.88e-21 94.7
MS.gene026953.t1 MTR_3g005970 31.104 299 165 12 386 662 72 351 2.73e-22 100
MS.gene026953.t1 MTR_3g005970 30.677 251 142 7 116 345 112 351 7.31e-21 95.9
MS.gene026953.t1 MTR_1g107480 31.897 232 146 5 122 349 119 342 3.74e-22 101
MS.gene026953.t1 MTR_1g107480 29.719 249 165 4 439 684 119 360 5.01e-22 101
MS.gene026953.t1 MTR_8g033390 27.413 259 173 5 428 681 65 313 1.08e-20 94.4
MS.gene026953.t1 MTR_8g033390 26.829 246 165 4 111 351 65 300 1.20e-19 91.3
MS.gene026953.t1 MTR_4g013100 25.267 281 193 5 408 684 40 307 1.15e-20 94.0
MS.gene026953.t1 MTR_4g013100 24.906 265 182 5 91 351 40 291 1.72e-18 87.4
MS.gene026953.t1 MTR_4g012960 26.275 255 176 4 431 684 60 303 2.82e-19 89.7
MS.gene026953.t1 MTR_4g012960 25.833 240 166 4 113 351 59 287 1.53e-17 84.7
MS.gene026953.t1 MTR_8g033400 26.667 255 165 7 111 358 76 315 7.33e-17 82.8
MS.gene026953.t1 MTR_8g033400 28.400 250 155 8 439 681 87 319 8.65e-17 82.8
MS.gene026953.t1 MTR_8g033410 25.517 290 179 9 412 681 40 312 3.71e-16 80.5
MS.gene026953.t1 MTR_2g101840 26.772 254 176 6 433 684 62 307 3.93e-14 74.3
MS.gene026953.t1 MTR_2g101840 27.004 237 163 6 113 347 59 287 5.67e-13 70.9
MS.gene026953.t1 MTR_4g013135 29.528 254 168 6 433 684 62 306 6.62e-13 70.9
MS.gene026953.t1 MTR_4g013135 29.536 237 158 5 116 351 62 290 1.24e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026953.t1 AT5G14780 83.616 354 58 0 350 703 31 384 0.0 598
MS.gene026953.t1 AT5G14780 74.866 374 88 3 1 371 1 371 0.0 538
MS.gene026953.t1 AT5G14780 83.616 354 58 0 350 703 17 370 0.0 598
MS.gene026953.t1 AT5G14780 78.592 341 71 1 33 371 17 357 0.0 533
MS.gene026953.t1 AT5G14780 83.616 354 58 0 350 703 5 358 0.0 597
MS.gene026953.t1 AT5G14780 78.592 341 71 1 33 371 5 345 0.0 533
MS.gene026953.t1 AT1G17745 29.070 258 178 4 428 684 134 387 1.31e-24 109
MS.gene026953.t1 AT1G17745 29.545 264 173 5 90 349 115 369 2.26e-24 108
MS.gene026953.t1 AT4G34200 31.169 231 149 3 122 349 125 348 1.12e-23 106
MS.gene026953.t1 AT4G34200 29.825 228 156 3 439 666 125 348 9.02e-23 103
MS.gene026953.t1 AT1G72190 30.508 236 155 3 116 345 110 342 2.79e-23 102
MS.gene026953.t1 AT1G72190 27.817 284 189 6 385 662 69 342 9.74e-23 101
MS.gene026953.t1 AT3G19480 29.565 230 156 4 121 349 109 333 1.01e-21 100
MS.gene026953.t1 AT3G19480 27.530 247 175 3 438 684 109 351 3.01e-21 99.0
MS.gene026953.t1 AT1G17745 27.465 284 176 6 428 684 134 414 1.17e-21 100
MS.gene026953.t1 AT1G17745 27.931 290 171 7 90 349 115 396 1.95e-21 99.8
MS.gene026953.t1 AT1G79870 27.843 255 175 5 433 686 62 308 1.38e-20 93.6
MS.gene026953.t1 AT1G79870 27.731 238 163 5 113 349 59 288 2.31e-18 87.0
MS.gene026953.t1 AT1G12550 25.106 235 170 2 428 662 64 292 1.40e-19 90.9
MS.gene026953.t1 AT1G12550 25.564 266 188 5 81 345 36 292 1.56e-19 90.9
MS.gene026953.t1 AT2G45630 27.935 247 171 4 109 354 78 318 2.81e-19 90.1
MS.gene026953.t1 AT2G45630 28.750 240 164 4 426 664 78 311 6.35e-19 89.4
MS.gene026953.t1 AT1G79870 25.490 255 162 5 433 686 62 289 7.19e-15 76.3
MS.gene026953.t1 AT1G79870 25.210 238 150 5 113 349 59 269 1.20e-12 69.7

Find 178 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACACATCCAACAAAAT+TGG 0.150781 6.2:-2710455 None:intergenic
TATGCTTGAGAGGCACTTTA+AGG 0.168046 6.2:+2716615 MS.gene026953:CDS
TTCAACACATCCAACAAAAT+TGG 0.168585 6.2:-2715200 None:intergenic
ATACAAAAGGTTACAGTTAA+AGG 0.172776 6.2:-2711133 None:intergenic
ATATAAAAGGTTACAGTTAA+AGG 0.184451 6.2:-2715782 None:intergenic
CCAATTTCTTTCTCCAATTC+AGG 0.191495 6.2:-2715826 None:intergenic
CAATGCTCGTGGGGCAATTA+TGG 0.213265 6.2:+2716113 MS.gene026953:CDS
GCCATGACTCCTCATATTTC+TGG 0.221909 6.2:+2716413 MS.gene026953:CDS
TAGCGCCATGGATGATCTTT+TGG 0.226552 6.2:-2711848 None:intergenic
GCTATGACTCCACATATTTC+TGG 0.241519 6.2:+2711884 MS.gene026953:CDS
CAATGCTCGTGGAGCACTTA+TGG 0.246792 6.2:+2711653 MS.gene026953:CDS
TAAATTATACCTTGTCTTAT+CGG 0.247196 6.2:-2715920 None:intergenic
CTCAACGCTGCGGCTGCTGC+TGG 0.289747 6.2:+2715532 MS.gene026953:CDS
TAGCGCCATGGATGATCTTT+TGG 0.296464 6.2:-2716377 None:intergenic
TTCACAATGTAGTGCTGTTC+AGG 0.309434 6.2:-2716649 None:intergenic
CCTGAATTGGAGAAAGAAAT+TGG 0.310313 6.2:+2715826 MS.gene026953:CDS
CCTGGATTGGAGAAAGAAAT+TGG 0.311248 6.2:+2711177 MS.gene026953:CDS
AGTTAAAGGGTTTCAGCCTT+TGG 0.316924 6.2:-2711119 None:intergenic
AGTTAAAGGGTTTCAGCCTT+TGG 0.316924 6.2:-2715768 None:intergenic
TATAAAAGGTTACAGTTAAA+GGG 0.334347 6.2:-2715781 None:intergenic
GGTGAAATGGTGTGGATATC+AGG 0.337349 6.2:-2710774 None:intergenic
TCAACACATCCAACAAAATT+GGG 0.339618 6.2:-2715199 None:intergenic
CGCTTTCTTAATTCTTTCCT+CGG 0.344856 6.2:-2715458 None:intergenic
TAAGTCTATCATGATATAAA+AGG 0.348710 6.2:-2715795 None:intergenic
GGTTACAAGCAGGCTGTTGC+CGG 0.355403 6.2:+2715649 MS.gene026953:CDS
GACAAAGAAGGACTCAATTC+TGG 0.356342 6.2:+2710538 MS.gene026953:CDS
CTCAATGCAGCGGCTGCTGC+TGG 0.358769 6.2:+2710883 MS.gene026953:CDS
GGTTACCATCAGGCTATTAC+TGG 0.361693 6.2:+2711000 MS.gene026953:CDS
TGTTCTAGTGGACACGTTGC+AGG 0.363087 6.2:+2711699 MS.gene026953:CDS
GACAAAGAAGGACCTGATTC+TGG 0.363375 6.2:+2715283 MS.gene026953:CDS
TACAAAAGGTTACAGTTAAA+GGG 0.364851 6.2:-2711132 None:intergenic
GATACTACATTCACCGGTAT+TGG 0.372695 6.2:-2716690 None:intergenic
TAGGTTACAGTGGTGATGTT+TGG 0.375068 6.2:+2711810 MS.gene026953:intron
TAGGTTACAGTGGTGATGTT+TGG 0.375068 6.2:+2716339 MS.gene026953:intron
ACCGGTATTGGCTTGCCAAT+TGG 0.379845 6.2:-2716678 None:intergenic
GGTCAGTTACGTTATGCTGC+TGG 0.379919 6.2:+2716583 MS.gene026953:intron
CCAATTTCTTTCTCCAATCC+AGG 0.384620 6.2:-2711177 None:intergenic
GTCAGTTACGTTATGCTGCT+GGG 0.386317 6.2:+2716584 MS.gene026953:intron
CTTTCCTCGGTGACATAGGC+AGG 0.386770 6.2:-2715445 None:intergenic
TCAAAATTCTAACCAGAATC+AGG 0.393287 6.2:-2715295 None:intergenic
TCAACACATCCAACAAAATT+GGG 0.405718 6.2:-2710454 None:intergenic
CGTCTTTCCTTCAAGATCAT+AGG 0.406591 6.2:-2715704 None:intergenic
ACTTAAGATGGAGCCTGAAT+TGG 0.406632 6.2:+2715813 MS.gene026953:CDS
GGTGAAATGGTGTAGATATC+AGG 0.417800 6.2:-2715423 None:intergenic
AGTCTTTCCTTCAAGATCAT+AGG 0.421953 6.2:-2711055 None:intergenic
TCCAACAAAATTGGGATTCA+AGG 0.426930 6.2:-2710446 None:intergenic
CTTGTGAGAAATTTCTTGCC+TGG 0.428851 6.2:+2710979 MS.gene026953:CDS
ATATCATGATAGACTTAAGA+TGG 0.430838 6.2:+2715801 MS.gene026953:CDS
CATTGTGAAGCAAGGCCAAT+TGG 0.431813 6.2:+2716663 MS.gene026953:CDS
ATAAACTACCTGTGCATCAA+TGG 0.432079 6.2:-2716441 None:intergenic
ACGGTTGGTGCTGGACGAAT+CGG 0.435730 6.2:+2711084 MS.gene026953:CDS
TGAGGAGTCATGGCATGATT+AGG 0.436581 6.2:-2716404 None:intergenic
ATGCTTGAGAGGCACTTTAA+GGG 0.439584 6.2:+2716616 MS.gene026953:CDS
GGAGGAAAGAAGAAAATTGT+AGG 0.439790 6.2:+2715139 MS.gene026953:CDS
CACCTTCTTCCATCATCACC+AGG 0.440843 6.2:+2708104 MS.gene026953:CDS
AGCAGGCTGTTGCCGGAGAA+TGG 0.441500 6.2:+2715656 MS.gene026953:CDS
CATAGAGCCTATGATCTTGA+AGG 0.442532 6.2:+2711048 MS.gene026953:CDS
CATAGAGCCTATGATCTTGA+AGG 0.442532 6.2:+2715697 MS.gene026953:CDS
GGATGTGTTGAAGGAGCATT+AGG 0.450524 6.2:+2710466 MS.gene026953:CDS
GGATGTGTTGAAGGAGCATT+AGG 0.450524 6.2:+2715211 MS.gene026953:CDS
TGTACTAGTGGACACATTGC+AGG 0.452738 6.2:+2716159 MS.gene026953:CDS
ATAAACTACCTGTGCATCAA+TGG 0.452777 6.2:-2711912 None:intergenic
TGTGGAGTCATAGCATGATT+AGG 0.457149 6.2:-2711875 None:intergenic
GTATCATGATAGACTTAAGA+TGG 0.461290 6.2:+2711152 MS.gene026953:CDS
GGTGGAAAGAAGAAGATTGT+AGG 0.462288 6.2:+2710394 MS.gene026953:CDS
CTTTCCGCAGTGACATAGGC+AGG 0.464618 6.2:-2710796 None:intergenic
TGTTGTTGAGAATAGGCCTC+TGG 0.466148 6.2:+2710373 MS.gene026953:intron
CTTTGAAGAAGCAGTGATCA+AGG 0.467599 6.2:-2708065 None:intergenic
CGGTTGGTGCTGGACGGATC+GGG 0.468797 6.2:+2715734 MS.gene026953:CDS
GTGACATAGGCAGGGTGAAA+TGG 0.470645 6.2:-2710787 None:intergenic
GTGACATAGGCAGGGTGAAA+TGG 0.470645 6.2:-2715436 None:intergenic
ACGGTTGGTGCTGGACGGAT+CGG 0.474630 6.2:+2715733 MS.gene026953:CDS
CTCGTGAGGAATTTCTTGCC+TGG 0.476350 6.2:+2715628 MS.gene026953:CDS
ACTGGAGAATGGAATGTTGC+TGG 0.476391 6.2:+2711018 MS.gene026953:CDS
TTTCTTGCCTGGTTACCATC+AGG 0.479444 6.2:+2710990 MS.gene026953:CDS
GCCGGAGAATGGAATGTTGC+TGG 0.480317 6.2:+2715667 MS.gene026953:CDS
TATTATTGAGAATAGGCCTC+TGG 0.487053 6.2:+2715118 MS.gene026953:intron
GATATCAGGACATGCAGATC+GGG 0.492666 6.2:-2715409 None:intergenic
ATGAAGGTCCCTGGTGATGA+TGG 0.493616 6.2:-2708113 None:intergenic
TAAGTCTATCATGATACAAA+AGG 0.497525 6.2:-2711146 None:intergenic
GTTGCTGATGCTTGTTCTAG+TGG 0.497709 6.2:+2711687 MS.gene026953:CDS
GCCAATTGGCAAGCCAATAC+CGG 0.498456 6.2:+2716677 MS.gene026953:CDS
ACGGTTGGAACGGTTGGTGC+TGG 0.499961 6.2:+2715724 MS.gene026953:CDS
ACTTAAGATGGAGCCTGGAT+TGG 0.500266 6.2:+2711164 MS.gene026953:CDS
ATCAGGCTATTACTGGAGAA+TGG 0.500716 6.2:+2711007 MS.gene026953:CDS
ACCAGCAACATTCCATTCTC+CGG 0.501972 6.2:-2715668 None:intergenic
ACCTTGTCTTATCGGTGAGA+GGG 0.507302 6.2:-2715912 None:intergenic
GATAGACTTAAGATGGAGCC+TGG 0.507973 6.2:+2711159 MS.gene026953:CDS
AATTCTTTCCTCGGTGACAT+AGG 0.513371 6.2:-2715449 None:intergenic
AGAATTGCTAAGTTGAAGAA+AGG 0.514648 6.2:+2711615 MS.gene026953:CDS
AGAAATTGGAGCTCAGTTTG+AGG 0.516756 6.2:+2711191 MS.gene026953:CDS
AATTCTTTCCGCAGTGACAT+AGG 0.516858 6.2:-2710800 None:intergenic
GCAACAGCCTGCTTGTAACC+AGG 0.517533 6.2:-2715646 None:intergenic
GGAAAGACGGTTGGAACGGT+TGG 0.521323 6.2:+2715718 MS.gene026953:CDS
TTGATTGTTAACAATGCTCG+TGG 0.521418 6.2:+2716102 MS.gene026953:CDS
GATCTTGAAGGAAAGACTGT+TGG 0.522138 6.2:+2711060 MS.gene026953:CDS
TTTCTTGCCTGGTTACAAGC+AGG 0.523136 6.2:+2715639 MS.gene026953:CDS
AGAAATTGGAGCTCAGTTTG+AGG 0.526267 6.2:+2715840 MS.gene026953:CDS
CGGTTGGTGCTGGACGAATC+GGG 0.528270 6.2:+2711085 MS.gene026953:CDS
GATATCAGGACATTGAGATC+AGG 0.531388 6.2:-2710760 None:intergenic
TGGAACTACCATTGATGCAC+AGG 0.534482 6.2:+2711904 MS.gene026953:CDS
ACTGTTGGAACGGTTGGTGC+TGG 0.535424 6.2:+2711075 MS.gene026953:CDS
TGGAACCACCATTGATGCAC+AGG 0.536965 6.2:+2716433 MS.gene026953:CDS
CACAGGAAGCAACGTAGTAT+CGG 0.541220 6.2:+2715573 MS.gene026953:CDS
TGAGAGGCACTTTAAGGGTG+AGG 0.541517 6.2:+2716621 MS.gene026953:CDS
AGAATCGCTAAGTTGAAGAA+AGG 0.545413 6.2:+2716075 MS.gene026953:CDS
ATAACAACTATGTCGCACTT+TGG 0.549637 6.2:-2715883 None:intergenic
GATCTTGAAGGAAAGACGGT+TGG 0.556559 6.2:+2715709 MS.gene026953:CDS
ACTTTGGTAGCATTGTATCA+AGG 0.557573 6.2:-2715867 None:intergenic
TACCTTGTCTTATCGGTGAG+AGG 0.560917 6.2:-2715913 None:intergenic
TCCAGAAATATGAGGAGTCA+TGG 0.561503 6.2:-2716414 None:intergenic
GGAAAGACTGTTGGAACGGT+TGG 0.564061 6.2:+2711069 MS.gene026953:CDS
TTAACCGTCGCAGAGGTCAC+AGG 0.565278 6.2:+2710907 MS.gene026953:CDS
TTAACCGTCGCAGAGGTCAC+AGG 0.565278 6.2:+2715556 MS.gene026953:CDS
ACATCCAACAAAATTGGGGT+TGG 0.565621 6.2:-2715194 None:intergenic
GTTGCTGATGCTTGTACTAG+TGG 0.565800 6.2:+2716147 MS.gene026953:CDS
ATTAGGTATACGCGAGTGGT+TGG 0.567034 6.2:+2710483 MS.gene026953:CDS
AATGTGATCAGAACCAATGC+CGG 0.567247 6.2:-2715506 None:intergenic
CGCGAGTGGTTGGAATCACA+AGG 0.567721 6.2:+2710493 MS.gene026953:CDS
CAGCTCCAAAAGATCATCCA+TGG 0.568481 6.2:+2716372 MS.gene026953:CDS
TGAGCTTACATGAAGGTCCC+TGG 0.575307 6.2:-2708122 None:intergenic
TACCTTGTCTTATCAGTGAG+AGG 0.576761 6.2:-2711264 None:intergenic
TGATTGTTAACAATGCTCGT+GGG 0.577213 6.2:+2716103 MS.gene026953:CDS
GTAATAGCCTGATGGTAACC+AGG 0.579644 6.2:-2710997 None:intergenic
AGATATCAGGACATGCAGAT+CGG 0.580334 6.2:-2715410 None:intergenic
ATTGTCACTGATGACAAAGA+AGG 0.582437 6.2:+2710526 MS.gene026953:CDS
ATGGTAGTTCCAGAAATATG+TGG 0.586451 6.2:-2711893 None:intergenic
TTGGAACGGTTGGTGCTGGA+CGG 0.591545 6.2:+2715728 MS.gene026953:CDS
TGAAGGAAAGACGGTTGGAA+CGG 0.596590 6.2:+2715714 MS.gene026953:CDS
ATTGTAGGTGTGTTCTACAA+AGG 0.596743 6.2:+2715154 MS.gene026953:CDS
AATCTTCTTCTTTCCACCAG+AGG 0.597365 6.2:-2710389 None:intergenic
TTTCATCTTATAGGTTACAG+TGG 0.598319 6.2:+2716329 MS.gene026953:intron
GCTTCCTGTGACCTCTGCGA+CGG 0.602109 6.2:-2710911 None:intergenic
CAGCCCCAAAAGATCATCCA+TGG 0.602826 6.2:+2711843 MS.gene026953:CDS
ATTGTCACAGATGACAAAGA+AGG 0.604616 6.2:+2715271 MS.gene026953:CDS
TTTCATCTTATAGGTTACAG+TGG 0.605311 6.2:+2711800 MS.gene026953:intron
CAACGTAGTATCGGTTGCTG+AGG 0.609487 6.2:+2715582 MS.gene026953:CDS
GCTTCCTGTGACCTCTGCGA+CGG 0.611055 6.2:-2715560 None:intergenic
TGATTAGGCATGTAGCGCCA+TGG 0.614868 6.2:-2711860 None:intergenic
TGATTAGGCATGTAGCGCCA+TGG 0.614868 6.2:-2716389 None:intergenic
TGAGAATTCTCATTCTCGTG+AGG 0.615649 6.2:+2715614 MS.gene026953:CDS
CTATGATCTTGAAGGAAAGA+CGG 0.616356 6.2:+2715705 MS.gene026953:CDS
CAACACTGTGTCGGTTGCTG+AGG 0.618658 6.2:+2710933 MS.gene026953:CDS
CAACACATCCAACAAAATTG+GGG 0.620958 6.2:-2715198 None:intergenic
CCTTGTCTTATCGGTGAGAG+GGG 0.620981 6.2:-2715911 None:intergenic
TATTGAGAATAGGCCTCTGG+AGG 0.626934 6.2:+2715121 MS.gene026953:intron
CCCCTCTCACCGATAAGACA+AGG 0.628167 6.2:+2715911 MS.gene026953:CDS
ATTCTCCAGTAATAGCCTGA+TGG 0.630819 6.2:-2711005 None:intergenic
AACTACCTGTGCATCAATGG+TGG 0.632641 6.2:-2716438 None:intergenic
ATTGTAGGTGTGTTCTACAA+AGG 0.635031 6.2:+2710409 MS.gene026953:CDS
CGCGAGTGGCTTGAATCACA+AGG 0.636363 6.2:+2715238 MS.gene026953:CDS
CACAGGAAGCAACACTGTGT+CGG 0.636460 6.2:+2710924 MS.gene026953:CDS
TTTCCTCGGTGACATAGGCA+GGG 0.636732 6.2:-2715444 None:intergenic
TGAAGGAAAGACTGTTGGAA+CGG 0.637197 6.2:+2711065 MS.gene026953:CDS
GATTGTTAACAATGCTCGTG+GGG 0.637460 6.2:+2716104 MS.gene026953:CDS
CAGCACTACATTGTGAAGCA+AGG 0.639193 6.2:+2716655 MS.gene026953:CDS
TTTCCGCAGTGACATAGGCA+GGG 0.641235 6.2:-2710795 None:intergenic
TTGATTGTCAACAATGCTCG+TGG 0.642232 6.2:+2711642 MS.gene026953:CDS
AATTGGAGCTCAGTTTGAGG+AGG 0.645215 6.2:+2711194 MS.gene026953:CDS
TGTTGAGAATAGGCCTCTGG+TGG 0.646139 6.2:+2710376 MS.gene026953:intron
TCCCTGGTGATGATGGAAGA+AGG 0.649337 6.2:-2708106 None:intergenic
ACCTTCTTCCATCATCACCA+GGG 0.652169 6.2:+2708105 MS.gene026953:CDS
ATAACAATAATGTCGCACTT+CGG 0.654035 6.2:-2711234 None:intergenic
GAGCATTAGGTATACGCGAG+TGG 0.657382 6.2:+2715224 MS.gene026953:CDS
TCACATTGATCTCAATGCAG+CGG 0.659822 6.2:+2710873 MS.gene026953:CDS
TGCTGGTTTAACCGTCGCAG+AGG 0.660243 6.2:+2710900 MS.gene026953:CDS
TGCTGGTTTAACCGTCGCAG+AGG 0.660243 6.2:+2715549 MS.gene026953:CDS
ATAGGCAGGGTGAAATGGTG+TGG 0.663821 6.2:-2710782 None:intergenic
GAGCATTAGGTATACGCGAG+TGG 0.665851 6.2:+2710479 MS.gene026953:CDS
TCAGTTACGTTATGCTGCTG+GGG 0.676617 6.2:+2716585 MS.gene026953:intron
TGCCTCTCACTGATAAGACA+AGG 0.678138 6.2:+2711262 MS.gene026953:CDS
GTAGGTGTGTTCTACAAAGG+CGG 0.684274 6.2:+2710412 MS.gene026953:CDS
ATGGTGGTTCCAGAAATATG+AGG 0.685776 6.2:-2716422 None:intergenic
GTGATCAGAACCAATGCCAG+CGG 0.706339 6.2:-2710854 None:intergenic
TCACATTGATCTCAACGCTG+CGG 0.717985 6.2:+2715522 MS.gene026953:CDS
GCGAGTGGTTGGAATCACAA+GGG 0.733708 6.2:+2710494 MS.gene026953:CDS
TCACCCTGCCTATGTCACTG+CGG 0.753926 6.2:+2710792 MS.gene026953:CDS
TCACCCTGCCTATGTCACCG+AGG 0.774835 6.2:+2715441 MS.gene026953:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr6.2 gene 2708040 2716702 2708040 ID=MS.gene026953
chr6.2 mRNA 2708040 2716702 2708040 ID=MS.gene026953.t1;Parent=MS.gene026953
chr6.2 exon 2708040 2708135 2708040 ID=MS.gene026953.t1.exon1;Parent=MS.gene026953.t1
chr6.2 CDS 2708040 2708135 2708040 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
chr6.2 exon 2710388 2710559 2710388 ID=MS.gene026953.t1.exon2;Parent=MS.gene026953.t1
chr6.2 CDS 2710388 2710559 2710388 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
chr6.2 exon 2710743 2711283 2710743 ID=MS.gene026953.t1.exon3;Parent=MS.gene026953.t1
chr6.2 CDS 2710743 2711283 2710743 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
chr6.2 exon 2711596 2711720 2711596 ID=MS.gene026953.t1.exon4;Parent=MS.gene026953.t1
chr6.2 CDS 2711596 2711720 2711596 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
chr6.2 exon 2711813 2711925 2711813 ID=MS.gene026953.t1.exon5;Parent=MS.gene026953.t1
chr6.2 CDS 2711813 2711925 2711813 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
chr6.2 exon 2715133 2715304 2715133 ID=MS.gene026953.t1.exon6;Parent=MS.gene026953.t1
chr6.2 CDS 2715133 2715304 2715133 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
chr6.2 exon 2715392 2715932 2715392 ID=MS.gene026953.t1.exon7;Parent=MS.gene026953.t1
chr6.2 CDS 2715392 2715932 2715392 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
chr6.2 exon 2716056 2716180 2716056 ID=MS.gene026953.t1.exon8;Parent=MS.gene026953.t1
chr6.2 CDS 2716056 2716180 2716056 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
chr6.2 exon 2716342 2716454 2716342 ID=MS.gene026953.t1.exon9;Parent=MS.gene026953.t1
chr6.2 CDS 2716342 2716454 2716342 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
chr6.2 exon 2716589 2716702 2716589 ID=MS.gene026953.t1.exon10;Parent=MS.gene026953.t1
chr6.2 CDS 2716589 2716702 2716589 ID=cds.MS.gene026953.t1;Parent=MS.gene026953.t1
Gene Sequence

>MS.gene026953

ATGGCTATGAAACGTGCTGCTTCAACCTTGATCACTGCTTCTTCAAAGATTTCATCACTTTCTTCACCTTCTTCCATCATCACCAGGGACCTTCATGTAAGCTCAATCTTTTCATTCATCTTAATTTCATTCAATCATTTGGCAATTTGTTTATTCATCTTTTCTTAATCAATTTTTATGCAATCATTAATATTATTAACAATTCAATTTATCCAAAAATAATAAAAAAAGATAAATTAAGAACTCAAAAGAAGCTTTGGTGTGATCTGGTATATGTCAATTTTCTTTGCTCTTTCTTGCATGCATCTTGATGATCCGTGACATTCACGCGATGTCAGTATGCCACCCCTTGTCTCTTTCAACAGTCAACACTTGTCACATATTGAGTACTCGTCAAACTTATCTTACTCCCTCCGTTTCAAAATGAGTGTCGTTTTAGGTGTTTACACACATATTAAGAAATGCAATGATTAAAAGAAAGAGAATGTTTATTTTACCAAATTATCCTTATTAACTATTGGTTAGTTTTAAAAGTAATACTTTGGAAGTAGTGTGCAGAGTATTAATTGGAGGGTACAATTGAAAAGAAAAAGTTATTATTGCATTGGAAACTGAAAGCGACATTCATTTTAAAAAAATTTTTTTGGCTAAAGCGACACTCATTTTGAAACGGAGGGAGTAAAAAAAAATCAAATAGTACATCAGTTAAGATAAGTTCAAAGAAATTAAAGTTGATCTCACCTTTTATAAATGAGTCTTAACGGTGGTCAATGAGAGAGTGGGTGTTTTTTTGTTGGCTGGAGTTGAAACCCAAAATCTTGCATATATGATGCATTATCACTAGCAATTGAGCTTAGCTAAGCTCAGAGGACACAGTGAGTTTTATTGAAAAGAGTGCGTTTTTAGACCTTATGTTTAATTAAGGGCAACATACATGGTATTTGTGAAAAAAAATCATATTTTTTAAAATTAAATAATTATTTTTAGAAGTTTTTTTTTTTGAAAAAAATCAAAATGGGATATATTAACAACGGCGGTGAAACACTCCTAGTGCAAGGTGTACTAAAGGAGCCACCCCGGAAAACAAATGTTTCCGTGCATTATTTTTTTAGGGTCATCCTTATATTATAATAAACCACTTTTAATTCCGTGAGTTTATCTCAGTTGGTATGGACAATGCATAATATATGCAATGTCCAGGGTTTCGGCAAAAAAAACCACTTTTAAAATTTAACATTAAAAGAAAATTAAAATAATCACTTTGAAAAGTTTTTATGAATATAAAACAATCACTTTCTAATTAAATAAAATAATCATTATATTACACTCCCTCCATTTCGAAATAAGTGTTGCTTTAGTCAAAAAAAATTGTTTCAAATGAATGTCGCTTTCAGTTTCCAATGCAATAATAACTTTTTCTTTTCAATTGTACCCTTCAATTAATACTCTGCACACTACTTCTAAAGTATTACTTTTAAAACTAACCAATAGTTAATAAGGATAATTTGGTAAAATAACCATTCTCTTTCTTTTAATCATTGCATTTCTTAATATGTGTGTAAACACCTAAAACGACACTCATTTCTTGTAAGTACATATATTAGAAGTGCAGTCCACGTAAGCTCTTCCAAACCCCCTTTTAAATTTTGTGGAATAAAATTGGTGTAGGGTACACCGTACACTATCCATTATTAAATGTGTGCAACCCTACATTAGTATCATCATTATACTTGTCTAGGACTAAATTTTTGGTGTAGCTAAAATAATAATGTTAAAAAAGTTTGTCCAATGAAATTAAATATCATATACAATTTCACCCGAAGCAACAGTTTTAGTAGTTTTATTTGGGTTTGTTTTACGAGGAGAAATCTATTTGTAACTCTGATAATTGTTAATTGGAAGAAAATGAGCCGTTAGTTTTATCTAACATTTCATTTTTATAGACATCCTTCATATGTAGTGGATCCGCCCATGTATTATAGTGAATTATTCATATAATATATTTAGTTGATTTAATGTTATCTTTTATGGGTAAACAAATATTTTAGTCTTTAAATGTGTGAAGCATTATCGGCGTAGTTTACAAGTTACTCTTTTGTTTGTAATTTTATAGATTAAATTGATCGGAAAAAGAAAAAAAAAACACACACACATTCAAAGACTAAAGTACCTAAAATGACTAACAAAATTTACATTTTTGTAATTTTGATCATTTAAGAACTATAATAACAATGTCTCATACATTCAGATATCAAAATGGTTGTTAAACCTGTCTTTTATCTATGCTTGTTTTGTTCTTTGACTTTTCTTATGCATTTCCATATATTCTTTTCTGTTGTTGAGAATAGGCCTCTGGTGGAAAGAAGAAGATTGTAGGTGTGTTCTACAAAGGCGGTGAATATGCTTCCTTGAATCCCAATTTTGTTGGATGTGTTGAAGGAGCATTAGGTATACGCGAGTGGTTGGAATCACAAGGGCATGAGTACATTGTCACTGATGACAAAGAAGGACTCAATTCTGGTTAGCATTATGCCAAATTAATCCACCCTCTTATTACGTCTATGCCGTAATTAAACTAATATATATACTTATGATTTTAAACATTTTTTCGGTTAAAATTTTACTTGAATACAAAAATATGCAGATAATATCTCAAGTTTGTGATTGCTTGTAACTTATGACTAATTGTTGTCCTGTTGCCAGAACTCGAGAAGCACATTCCTGATCTCAATGTCCTGATATCCACACCATTTCACCCTGCCTATGTCACTGCGGAAAGAATTAAGAAAGCTAAGAATTTAGAGCTGCTTTTAACCGCTGGCATTGGTTCTGATCACATTGATCTCAATGCAGCGGCTGCTGCTGGTTTAACCGTCGCAGAGGTCACAGGAAGCAACACTGTGTCGGTTGCTGAGGATGAGCTCATGAGAATTCTCATTCTTGTGAGAAATTTCTTGCCTGGTTACCATCAGGCTATTACTGGAGAATGGAATGTTGCTGGCATTGCACATAGAGCCTATGATCTTGAAGGAAAGACTGTTGGAACGGTTGGTGCTGGACGAATCGGGAAACTTTTACTCCAAAGGCTGAAACCCTTTAACTGTAACCTTTTGTATCATGATAGACTTAAGATGGAGCCTGGATTGGAGAAAGAAATTGGAGCTCAGTTTGAGGAGGATCTTGATGCTATGCTTCCGAAGTGCGACATTATTGTTATGAACATGCCTCTCACTGATAAGACAAGGTATAATTTTAATACTAATAGCATTCTATTCAAGTTATTTTTGACGCTTGAAATCTGAAGTCAATAAAGGAATTCATAGATATTCCAATATCAAAATGACTTGGTTTTGAATGATGTGTGTGAGTATGTATTCCATACGTTGTGTATTCAAGCTTACGGTTAGAGATTATAACCACTGCTTAATAGTTAATACTACGATCTAGTTTACAAGATTACCTCTATCAACTTATTAGTAAACTATGAGTATTACTCAAAACTACGAGTCATAATCAACAACAGTGACTGATTTTATTTTTATTTTTTGTGATTCAGAGGATTGTTTGACAAAAATAGAATTGCTAAGTTGAAGAAAGGTGTCTTGATTGTCAACAATGCTCGTGGAGCACTTATGGATACACAAGCAGTTGCTGATGCTTGTTCTAGTGGACACGTTGCAGGTAATTCTAAAACCAGCTGACATAAAATGTGTTTCGAAGCAAAACAAAAATCTATGTTTGAAGTAATGTAACACCCGAGTTTCATCTTATAGGTTACAGTGGTGATGTTTGGTTCCCACAACCAGCCCCAAAAGATCATCCATGGCGCTACATGCCTAATCATGCTATGACTCCACATATTTCTGGAACTACCATTGATGCACAGGTAGTTTATCTTCGACAGAATTACGTCACTCCTTTATCTTAATTTCTAGTTTCTGAGTTAGTCCTTTAATTCTTTTTCTCTTTTCATTCTACCCTCACACTTGCATGCATGCTCTTAAATGATAATTTTGAGGCAAACTGTAGGGCCATGTACTATGAGAAATGCAAACAATCATGTAGCACCGACACTTCAGATTGTAGGCGTGTCTGGCGTCCGACACGTGTTAATGTCCAATGATAATTTTGAGGCAAACTGTAGGGCCATATACTAGGAGAAATGCAAACAATCGTGTAGCACCGACACGTGTTAATGTCCAACATGTGTCAGTGTTACCAATGTTTGTGTCCTTGTTTGTGCTTCATAGATACAATGTGATTATGTGATACATCAACTGTGTCACGAGTCAAAATCCAAAACTGTTTTAGAATGCCAACATTGTTAATGTTCTAGACTATGCAGAACTAGATTGAAACTAAAATAAATACTGAAATGAGTAACGTTAAACTGGTTAAGATAAAGGACTAATCGAGCAATTTCGTCTTTAGTTTTCTAGACATTAGCATACATACCCTTGTCCCTTGTTCTTTGGTTAACTTCTTTGACTACATTTTCTGCATTTTTTGGTGAAATACTTATGATGATAATTGAAATGTGTTCAGTTACGTTATGCTGCTGGAGTCAAAGATATGCTTGAGAGGCACTTTAAGGGTGAAGACTTTCCTGAACAGAACTACATTGTGAAGGAGGGTCAATTGGCAAGCCAATACCGGTGAATGTAGTATCGAAGAGTGGTTCTGACTTCTGAGTCTTGTGGTTTTAAGGCCAATGAATTACTAAATAAAGATATCCGCGGCATTGTAATAATGTACTCAGCTTTGTTGAGATTGAAACTTGTTTAATAAAACTGCAATGGACACAAGTATCATTCTTGTGTTTTTTCTGAATTTGAATGTTCTGTTAGTAATTAGTGTATGTTTAAATTGAAACAATTCAACCTTTCAATTAAAAATATTTATCATGGTTATCAACTCCACAAGATGTCATCTTTCAGAAACTGTTTCGTCAAATTTATAACATTTGAGTTTGAATACCTTTTTTAGGCTAGAGAACTCATATCAGTTCATTATGAATAATATCTTTCATACATAGAGTTGCAACATATGGTCTTAAGTTTGAGGTTGGAACAAACTCAAATATAAAACCTTTTATTACATGACCTAACTCATGATTCAAAAGTATGTGTATTAAAATGATCTATCTGTGATGCACAGACACAAATTAATGTAATTGTATGTGTCAGTGTCAAACACAGACATGTGTCGAATGTCAAACACGTCTTTGATCAAAAGTGTCCGTGCTTCAGAGTCTACTACTTACTACTTTGGAATCTAATTCAAAAATCATGTCATCATCTTCCAAATCTCTAATCCAAAAGAATAGCTCGCAGCAGCCCTGTCTTTCAGCGATTTGAAGCTTGGACAAATTTTTATTATATTATTATATAAAAATGTATACAAGTGTTATATATCTACTACTTAGTGAGAAGTGTGATTTCTAGTTTTATCATATAATAATTCATTTTTCCTAATTATAAGACTATTTATAAAAATTGTTTGTCTCTTAATTTAAGATCCTCTACAAATTTTTAGATGTATTTATTACTCTTTTTCTGAACATATCTCTGATAAAAACTACCAATTTGTCTTCCAATATGAAATATTAATATTGAATGTGTTTATACATCAAGAGTAATTTGGTAATATTTATACATATTTTTTTTAATTTTAATAAAAAAATTAACAATCATTTCACTCTATGTATTTGACCCGTTTATTTCTTGGTTTCATTTTTCTTAATCAATTAAGTTCTCATTAATATTACAGAATAGACGAGTGTTACATACATGACTCATCTAACTTTTCTCTACTCTGCTTTCTTTTTATGTTGGCATAATTATAGTTATACATCTCTGTTTTCGCTCATGAAATTGGTCCCTCAATTTTAAAATCATACCGTTTTGATCCATTTTCATATTTTTAACTAAAAAAATAATGATGTGACATATTTTAGGTGACGTGTTATATTACATAACCTGATGATGTGAGATTATTGACAACCATGAAATTACAATAAAATTCCTTGAAAATTCTTCTGCACATTTTGTTGTTTCTTTTTATGGGCTATTATTATGCAACTTTTTCCTCCCAAATGTTTGGTCCAAAAGCATGGTGTCTTTGGCATCACAAAGGCATCCAAGCGTGATCCGTAAATACAGTATGATGAACTTATTTATGTTTCCTTTCTTGTGCTTGAACTACATCACATTTCTTTCTTCTTGGACAATTTGTGAGTTCTTTTCAATTTTGAGTGAAGTTTGAAAAAATGGTTTGTTTTCTTACCTTTTATCTTTGTTTTAGGATTAAGTTTGATTATATTATCCTACGATCAATGATTTGACTATCAAAACACGGTTTCTCATGTTTGATCACATGATAGTTATGTTTTCTCATGTTCCACATCCAAGTCCATGTACATGATTCCGTACTATCTATATGCACACCATAACATTTGTTTCCTAACTGTCGATAAATATATGAACAATAAAGATTTCTAGAAAATGACGTACAAGTGGAAGGGCACATTTAGAAATCATGTGCTCTTCTTTCTTTGTTTTTCTCTTTCTTTTTGTGTTTTTTAATCTTTAAAATTGGTAATATCCATGCACGAGGACACTTAGAGATGTCACATCATTATAAATTGAGGGAAGATGTGGGAGGCATCATGAAATTGCACAATTGAGAAACTTGAAGGGTTAAAATCGCCTAAAAAAATCAAGGGGTCAAAATCGGACAATTAGAAAACTTAGGGAGTCAAGAGTGCAATTAAGCTTTAATGTTATCATTTACGGGTAGAAAATTATTTTAGTCTTTTAATGTGTGACGCATTATCAATGTAGTTTACAAGTTACTCTTTTGTGTTCGTAATTTTATTGATTAAATTGATCAACGAAAAACATTTTCAAAGATTAAAGTAATTAAAATGACTAACAAAACTTATATTTAGATATAATTTTGTAATTTTGATACATTCAATGGCAGCTTATACATTCAGATATCAAAATGGTTGTTTTACCGGTATTTTCTGTATGTTTTGTTTTGTTCATTGATTTTCTTATACATTGCCATTTTTTGTTATTATTGAGAATAGGCCTCTGGAGGAAAGAAGAAAATTGTAGGTGTGTTCTACAAAGGCAATGAGAATGCTGCATCCAACCCCAATTTTGTTGGATGTGTTGAAGGAGCATTAGGTATACGCGAGTGGCTTGAATCACAAGGACATGAATACATTGTCACAGATGACAAAGAAGGACCTGATTCTGGTTAGAATTTTGACGAATTAATCGACCCTCTTTAAACTTTATCTTAAAAGTTTTTGGTTGCATTAACTTATGACTATCTGTTACCAGAACTCGAGAAGCACATTCCCGATCTGCATGTCCTGATATCTACACCATTTCACCCTGCCTATGTCACCGAGGAAAGAATTAAGAAAGCGAAGAATTTACAGTTGCTTTTAACTGCCGGCATTGGTTCTGATCACATTGATCTCAACGCTGCGGCTGCTGCTGGTTTAACCGTCGCAGAGGTCACAGGAAGCAACGTAGTATCGGTTGCTGAGGATGAGCTTATGAGAATTCTCATTCTCGTGAGGAATTTCTTGCCTGGTTACAAGCAGGCTGTTGCCGGAGAATGGAATGTTGCTGGTATTGCACATAGAGCCTATGATCTTGAAGGAAAGACGGTTGGAACGGTTGGTGCTGGACGGATCGGGAAACTTTTACTCCAAAGGCTGAAACCCTTTAACTGTAACCTTTTATATCATGATAGACTTAAGATGGAGCCTGAATTGGAGAAAGAAATTGGAGCTCAGTTTGAGGATGACCTTGATACAATGCTACCAAAGTGCGACATAGTTGTTATTAACACCCCTCTCACCGATAAGACAAGGTATAATTTAATACTATTATCGATCTATTAACGGTCTAGTTTACAAGGTTATCCATGTCCATTATTATGTAAACTATGAGTCATACCTAGCAATGAGAAAACTTTTTTCTTTTTGTGATTCAGAGGATTGTTTGACAAAAATAGAATCGCTAAGTTGAAGAAAGGCGTCTTGATTGTTAACAATGCTCGTGGGGCAATTATGGACACACAAGCAGTTGCTGATGCTTGTACTAGTGGACACATTGCAGGTAATTCTGAAATCAGCTGACAAAAAAAAAATTGTTTACGAACAGAACATAAAACGATGTTTCAAGTAATGTAAAACCTCGATTTCATTTATGTTTCTGAAAAAAATATGAAACTATGTTTGAACTTTGAAGTAATGTAACAGCTAAGTTTCATCTTATAGGTTACAGTGGTGATGTTTGGTTCCCACAACCAGCTCCAAAAGATCATCCATGGCGCTACATGCCTAATCATGCCATGACTCCTCATATTTCTGGAACCACCATTGATGCACAGGTAGTTTATTTTACTTTTTTTCTTGACATTAGCATACCTTTTTTCTTTAGCTAAGTTCATTGACCGACTACCGACTACATTTTCTCCATTTTTTTTGCGCAAATTACTGATATTGATAATTGAAACTTGGTCAGTTACGTTATGCTGCTGGGGTGAAAAATATGCTTGAGAGGCACTTTAAGGGTGAGGACTTTCCTGAACAGCACTACATTGTGAAGCAAGGCCAATTGGCAAGCCAATACCGGTGA
Protein sequence

>MS.gene026953.t1

MAMKRAASTLITASSKISSLSSPSSIITRDLHASGGKKKIVGVFYKGGEYASLNPNFVGCVEGALGIREWLESQGHEYIVTDDKEGLNSELEKHIPDLNVLISTPFHPAYVTAERIKKAKNLELLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNTVSVAEDELMRILILVRNFLPGYHQAITGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKMEPGLEKEIGAQFEEDLDAMLPKCDIIVMNMPLTDKTRGLFDKNRIAKLKKGVLIVNNARGALMDTQAVADACSSGHVAGYSGDVWFPQPAPKDHPWRYMPNHAMTPHISGTTIDAQASGGKKKIVGVFYKGNENAASNPNFVGCVEGALGIREWLESQGHEYIVTDDKEGPDSELEKHIPDLHVLISTPFHPAYVTEERIKKAKNLQLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGYKQAVAGEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKMEPELEKEIGAQFEDDLDTMLPKCDIVVINTPLTDKTRGLFDKNRIAKLKKGVLIVNNARGAIMDTQAVADACTSGHIAGYSGDVWFPQPAPKDHPWRYMPNHAMTPHISGTTIDAQLRYAAGVKNMLERHFKGEDFPEQHYIVKQGQLASQYR