AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027986

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027986.t1 MTR_5g028450 74.917 303 62 2 569 858 1 302 3.43e-151 446
MS.gene027986.t1 MTR_5g028510 69.085 317 91 3 107 422 18 328 8.59e-141 421
MS.gene027986.t1 MTR_5g028190 58.592 355 124 5 511 850 185 531 6.49e-132 405
MS.gene027986.t1 MTR_5g028190 47.489 219 87 5 205 421 5 197 9.22e-45 170
MS.gene027986.t1 MTR_5g028930 52.408 353 153 4 518 856 270 621 1.46e-114 363
MS.gene027986.t1 MTR_5g028930 48.084 287 121 10 126 407 1 264 2.01e-60 217
MS.gene027986.t1 MTR_5g028130 52.493 341 138 5 528 850 1 335 9.36e-114 351
MS.gene027986.t1 MTR_5g028690 56.250 208 89 2 652 858 36 242 4.62e-72 237
MS.gene027986.t1 MTR_5g028110 52.604 192 68 3 659 850 4 172 9.99e-56 190
MS.gene027986.t1 MTR_2g438740 38.305 295 161 4 572 851 279 567 3.77e-54 198
MS.gene027986.t1 MTR_6g017215 52.551 196 81 4 653 848 2 185 5.04e-52 182
MS.gene027986.t1 MTR_5g091220 34.808 339 205 5 527 853 270 604 1.21e-51 191
MS.gene027986.t1 MTR_3g055310 32.390 318 199 4 527 832 14 327 2.88e-51 184
MS.gene027986.t1 MTR_3g055310 32.390 318 199 4 527 832 77 390 1.39e-50 184
MS.gene027986.t1 MTR_3g055310 32.390 318 199 4 527 832 129 442 5.93e-50 183
MS.gene027986.t1 MTR_3g055310 32.390 318 199 4 527 832 157 470 7.21e-50 184
MS.gene027986.t1 MTR_3g055310 32.390 318 199 4 527 832 155 468 1.06e-49 183
MS.gene027986.t1 MTR_3g055310 32.390 318 199 4 527 832 182 495 1.89e-49 183
MS.gene027986.t1 MTR_5g028840 46.078 204 108 2 657 859 2 204 3.15e-49 173
MS.gene027986.t1 MTR_5g028900 55.357 168 61 4 658 824 3 157 4.31e-48 168
MS.gene027986.t1 MTR_2g438440 36.913 298 170 5 567 851 394 686 2.99e-47 180
MS.gene027986.t1 MTR_2g438400 36.149 296 169 6 572 852 258 548 1.89e-46 175
MS.gene027986.t1 MTR_2g438720 36.120 299 172 4 567 851 399 692 1.18e-45 176
MS.gene027986.t1 MTR_5g028830 36.601 306 140 8 366 656 22 288 1.82e-45 165
MS.gene027986.t1 MTR_2g438700 36.213 301 169 6 567 851 397 690 8.51e-45 173
MS.gene027986.t1 MTR_2g438760 34.237 295 174 5 572 851 273 562 3.97e-44 169
MS.gene027986.t1 MTR_3g455790 29.755 326 213 3 520 833 258 579 4.73e-43 166
MS.gene027986.t1 MTR_2g438640 34.899 298 176 4 567 851 399 691 1.52e-41 163
MS.gene027986.t1 MTR_2g020510 32.653 294 174 4 569 846 390 675 2.38e-40 159
MS.gene027986.t1 MTR_2g438640 35.088 285 167 4 567 851 399 665 1.59e-39 157
MS.gene027986.t1 MTR_2g020470 31.699 306 182 5 553 846 367 657 9.91e-38 151
MS.gene027986.t1 MTR_2g020450 33.028 327 189 7 552 857 372 689 3.45e-37 149
MS.gene027986.t1 MTR_3g452020 28.481 316 210 3 529 832 92 403 6.75e-37 144
MS.gene027986.t1 MTR_3g452020 28.481 316 210 3 529 832 268 579 4.18e-36 145
MS.gene027986.t1 MTR_3g058570 31.438 299 184 2 568 849 83 377 5.47e-34 135
MS.gene027986.t1 MTR_2g017930 35.517 290 167 5 562 834 352 638 6.19e-33 136
MS.gene027986.t1 MTR_5g028920 47.586 145 59 3 529 659 86 227 1.24e-30 121
MS.gene027986.t1 MTR_2g438640 38.462 195 115 2 657 851 399 588 5.99e-30 126
MS.gene027986.t1 MTR_5g085360 31.481 216 140 3 645 859 530 738 3.78e-27 118
MS.gene027986.t1 MTR_5g085340 27.541 305 187 5 569 849 443 737 8.94e-27 117
MS.gene027986.t1 MTR_3g050500 26.298 289 193 4 568 839 427 712 1.71e-26 116
MS.gene027986.t1 MTR_5g085350 27.551 294 191 4 570 846 438 726 4.92e-26 115
MS.gene027986.t1 MTR_5g085350 27.551 294 191 4 570 846 425 713 5.14e-26 115
MS.gene027986.t1 MTR_3g051060 28.095 210 148 2 643 852 524 730 6.69e-23 105
MS.gene027986.t1 MTR_1g113820 28.862 246 158 6 567 797 274 517 3.89e-18 89.4
MS.gene027986.t1 MTR_1g113760 26.697 221 147 3 560 766 231 450 4.89e-15 79.3
MS.gene027986.t1 MTR_2g438470 35.211 142 87 4 596 733 357 497 7.44e-15 78.6
MS.gene027986.t1 MTR_1g113730 24.000 250 173 5 560 794 37 284 6.57e-13 71.6
MS.gene027986.t1 MTR_2g438580 37.805 82 50 1 672 753 3 83 7.35e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027986.t1 AT3G18670 41.143 350 187 6 516 851 295 639 9.48e-76 260
MS.gene027986.t1 AT3G18670 29.767 215 109 6 219 427 88 266 2.04e-17 87.4
MS.gene027986.t1 AT3G18670 41.716 338 178 6 528 851 252 584 1.20e-75 258
MS.gene027986.t1 AT3G18670 28.500 200 101 6 219 412 88 251 1.17e-12 72.0
MS.gene027986.t1 AT5G04730 38.012 342 189 7 527 851 254 589 3.61e-59 213
MS.gene027986.t1 AT5G04700 38.484 343 187 8 526 851 321 656 1.05e-56 207
MS.gene027986.t1 AT5G04690 35.693 339 195 7 527 851 283 612 2.16e-53 197
MS.gene027986.t1 AT5G04690 35.693 339 195 7 527 851 303 632 4.38e-53 196
MS.gene027986.t1 AT5G04690 35.693 339 195 7 527 851 317 646 5.34e-53 196
MS.gene027986.t1 AT5G35810 35.762 302 171 7 569 851 35 332 6.72e-53 188
MS.gene027986.t1 AT5G04680 36.080 352 191 9 526 851 185 528 1.38e-51 190
MS.gene027986.t1 AT5G04680 36.080 352 191 9 526 851 294 637 7.50e-51 190
MS.gene027986.t1 AT5G04680 36.080 352 191 9 526 851 308 651 9.55e-51 190
MS.gene027986.t1 AT5G04680 36.080 352 191 9 526 851 337 680 1.60e-50 190
MS.gene027986.t1 AT3G54065 43.694 222 107 5 643 858 8 217 7.79e-48 170

Find 179 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTGAATCTGGGTCATTT+TGG 0.129048 5.2:+19484645 None:intergenic
ACTTACTTTCATAAATAATT+TGG 0.159583 5.2:+19482576 None:intergenic
CTGTCCATCCTTCATCTTCT+TGG 0.177247 5.2:+19484754 None:intergenic
CATTATCGACATGATCTTAA+AGG 0.186021 5.2:-19476795 MS.gene027986:CDS
CAGAACAATTCTTTATTCTT+TGG 0.203069 5.2:+19483376 None:intergenic
GAGTATGATATTGCCGAAAT+TGG 0.213255 5.2:-19479719 MS.gene027986:CDS
TGTATCACTTCTGAAATATT+TGG 0.223472 5.2:-19482926 MS.gene027986:intron
ATCTGCAGGGGCAAGGTCTA+TGG 0.241341 5.2:-19484820 MS.gene027986:CDS
GTACTACAATGAACCACTTA+TGG 0.252983 5.2:+19476762 None:intergenic
AAGAGAGATTCAATGGTTTA+AGG 0.258197 5.2:-19477765 MS.gene027986:intron
CGTTGTTCTAATAATGCTTT+CGG 0.260371 5.2:-19479591 MS.gene027986:CDS
TACCATGCACTTTGCTATTT+CGG 0.263241 5.2:+19483135 None:intergenic
TAGAACAAACAGAGAGAAAA+AGG 0.268023 5.2:+19476840 None:intergenic
TGATATCGATATTTCTCTTT+TGG 0.270087 5.2:-19479385 MS.gene027986:CDS
GTTGTTCTAATAATGCTTTC+GGG 0.284513 5.2:-19479590 MS.gene027986:intron
TCAACGGCTGAAAGAAAATT+AGG 0.286805 5.2:+19477988 None:intergenic
CAGCAACACATGTTACCTTT+TGG 0.289716 5.2:+19479687 None:intergenic
TACCGACCTTGCTCCAAATC+TGG 0.291922 5.2:+19484617 None:intergenic
GCGCTTGTTGCACGTTATAC+TGG 0.297367 5.2:-19483163 MS.gene027986:CDS
CAGTCTGGATAACGCCTAAA+AGG 0.301425 5.2:+19483690 None:intergenic
CAAAACTCGATTCTGTGAAT+TGG 0.302690 5.2:+19482961 None:intergenic
TCTGGGGCAGCTCTTGAATC+TGG 0.304986 5.2:+19484635 None:intergenic
TTCAAGAGCTGCCCCAGATT+TGG 0.307698 5.2:-19484630 MS.gene027986:CDS
AATCATTTAGGCTCATTATA+TGG 0.310793 5.2:-19483539 MS.gene027986:CDS
TTGGACGAAGTTCCCAATTT+CGG 0.318636 5.2:+19479706 None:intergenic
TTTACGTACCATTACTATCT+AGG 0.330681 5.2:+19479148 None:intergenic
TATATATGACATCGGCTTCC+AGG 0.333476 5.2:-19483303 MS.gene027986:CDS
CAGTACGAGATTCAGCATTC+TGG 0.335749 5.2:+19484786 None:intergenic
GTGCATGGTAGAGGAAATAA+AGG 0.338215 5.2:-19483122 MS.gene027986:CDS
TAAATGATTCCAGGCGCTTT+TGG 0.340866 5.2:+19483553 None:intergenic
ATTCTTCTAGCTGCTGCTAA+TGG 0.346856 5.2:-19483049 MS.gene027986:CDS
AATTCGAAACTGATGCTTCA+TGG 0.348970 5.2:-19483661 MS.gene027986:CDS
CCGTGGAGTTGCATATTTGT+TGG 0.349278 5.2:-19479187 MS.gene027986:CDS
GGCACCCGATTGGGATTTCT+AGG 0.351879 5.2:-19482599 MS.gene027986:CDS
TTGCACCTGGCAGCACATTT+AGG 0.356259 5.2:-19477836 MS.gene027986:intron
GACATACCCAAAAGCTTTAC+TGG 0.361682 5.2:+19479362 None:intergenic
ACTAAGGCTGAGAATTTATC+AGG 0.362851 5.2:+19482656 None:intergenic
TTACCTTGGAAGTTATTGTT+TGG 0.363753 5.2:-19476872 MS.gene027986:CDS
ATTTATCAGGTAAGGATTGC+TGG 0.365909 5.2:+19482669 None:intergenic
CAAAGAATAAAGAATTGTTC+TGG 0.367690 5.2:-19483375 MS.gene027986:CDS
TACTACAATGAACCACTTAT+GGG 0.368135 5.2:+19476763 None:intergenic
CATACGAAAATATGCCTCTA+TGG 0.371634 5.2:+19477959 None:intergenic
TTTGTCAAACACTTTGGATT+TGG 0.373648 5.2:+19482985 None:intergenic
TACACACATTATGAAGTTAT+AGG 0.373654 5.2:-19478147 MS.gene027986:CDS
TGATGCTTCATGGAAAATAA+AGG 0.374254 5.2:-19483651 MS.gene027986:CDS
CTTTAAGATCATGTCGATAA+TGG 0.388292 5.2:+19476796 None:intergenic
TTAGCAGCAGCTAGAAGAAT+AGG 0.397885 5.2:+19483052 None:intergenic
GCAACATCTTATACAATTGT+AGG 0.407639 5.2:-19477106 MS.gene027986:CDS
CTTCAATTATGTCAGGAATC+AGG 0.410277 5.2:-19478188 MS.gene027986:intron
GCTGCAGCCTTTACAGTGCC+AGG 0.415001 5.2:-19477058 MS.gene027986:CDS
TTTCCAAGTGGCACCCGATT+GGG 0.417478 5.2:-19482608 MS.gene027986:CDS
GAACTCGATTATTCCGTGTT+GGG 0.428128 5.2:+19478035 None:intergenic
AGTTGTCTTTAGTGAGTACT+TGG 0.432427 5.2:+19484873 None:intergenic
CTTTCAATGAATATCTCATA+AGG 0.433992 5.2:+19477175 None:intergenic
GGTGGAAACGACGACAAAAC+TGG 0.435847 5.2:-19477037 MS.gene027986:CDS
ATTGGATTTGTCAAACACTT+TGG 0.438556 5.2:+19482979 None:intergenic
TAGTGAAAGATGGAGAGAAA+TGG 0.438852 5.2:-19477141 MS.gene027986:CDS
TCAAACACTTTGGATTTGGT+GGG 0.439060 5.2:+19482989 None:intergenic
ATTATCGACATGATCTTAAA+GGG 0.440142 5.2:-19476794 MS.gene027986:CDS
GATATTACAAGAACGATAAT+CGG 0.441300 5.2:+19476716 None:intergenic
GTCAAACACTTTGGATTTGG+TGG 0.443427 5.2:+19482988 None:intergenic
CTCCGTCCCTACGTTGTTGA+GGG 0.447774 5.2:-19482735 MS.gene027986:CDS
AGTATGATATTGCCGAAATT+GGG 0.448600 5.2:-19479718 MS.gene027986:CDS
GGCTGAGAATTTATCAGGTA+AGG 0.449871 5.2:+19482661 None:intergenic
CAAACACTTTGGATTTGGTG+GGG 0.451286 5.2:+19482990 None:intergenic
TTTCGAATTCTGAAACAGTC+TGG 0.454616 5.2:+19483675 None:intergenic
TAATGAGCCTAAATGATTCC+AGG 0.455349 5.2:+19483544 None:intergenic
CCCAGAAACGTTGCTAGAAA+AGG 0.455513 5.2:-19483095 MS.gene027986:CDS
ATTCTCAGCCTTAGTTGCAT+TGG 0.455918 5.2:-19482648 MS.gene027986:CDS
GTGATGAGAGGTACGCCTAT+TGG 0.457145 5.2:-19483349 MS.gene027986:CDS
GGTACGCCTATTGGAAATAT+CGG 0.457767 5.2:-19483340 MS.gene027986:CDS
ACCGACCTTGCTCCAAATCT+GGG 0.458528 5.2:+19484618 None:intergenic
CTTCACGCAGCAGTGATTGC+TGG 0.458933 5.2:-19483271 MS.gene027986:CDS
TGCTGAATCTCGTACTGATC+AGG 0.459930 5.2:-19484780 MS.gene027986:CDS
AGTAGTATGATGAAATCAAC+AGG 0.460104 5.2:-19484920 None:intergenic
CAACTCATACATTCTGTCAA+TGG 0.460886 5.2:-19477907 MS.gene027986:CDS
TCATTTGTTTATTGAACGGA+TGG 0.471650 5.2:+19476646 None:intergenic
TACCATTACTATCTAGGTAA+TGG 0.473551 5.2:+19479154 None:intergenic
TTAAAACAGCCAAAAGCGCC+TGG 0.474873 5.2:-19483562 MS.gene027986:CDS
AAAAGCGCCTGGAATCATTT+AGG 0.476909 5.2:-19483551 MS.gene027986:CDS
AAATGCAAAGAGAGATTCAA+TGG 0.476952 5.2:-19477772 MS.gene027986:CDS
AAACACTTTGGATTTGGTGG+GGG 0.483841 5.2:+19482991 None:intergenic
TTGCATTTGCAGAGCAGCAC+CGG 0.486301 5.2:+19477787 None:intergenic
TGTGGAAAATGTGGAATTGT+TGG 0.486891 5.2:-19483245 MS.gene027986:CDS
TAGCAGCAGCTAGAAGAATA+GGG 0.490849 5.2:+19483053 None:intergenic
GTCGTCATTTGTTTATTGAA+CGG 0.491644 5.2:+19476642 None:intergenic
AATTGGGAACTTCGTCCAAA+AGG 0.492844 5.2:-19479702 MS.gene027986:CDS
TGTTATGCAAAACTATCTGC+AGG 0.494906 5.2:-19484834 MS.gene027986:CDS
TATATTGATAATGATTGCTC+GGG 0.495481 5.2:+19482764 None:intergenic
CTGGGGCAGCTCTTGAATCT+GGG 0.496567 5.2:+19484636 None:intergenic
AGTGAAAGATGGAGAGAAAT+GGG 0.498200 5.2:-19477140 MS.gene027986:CDS
ATATATTGATAATGATTGCT+CGG 0.502639 5.2:+19482763 None:intergenic
CCTTTCTGCCTATGCCACAT+TGG 0.504501 5.2:+19479624 None:intergenic
AATCCCAATCGGGTGCCACT+TGG 0.507477 5.2:+19482605 None:intergenic
CTAAAGACAACTATGAAAGA+TGG 0.509180 5.2:-19484862 MS.gene027986:CDS
TGAACTCGATTATTCCGTGT+TGG 0.510589 5.2:+19478034 None:intergenic
TGCTGGAAATGTGGAAAATG+TGG 0.512870 5.2:-19483254 MS.gene027986:CDS
ATACTCCCAAGTGCCATGAT+TGG 0.513042 5.2:+19476740 None:intergenic
CATCTTTCACTAGCTCCTCA+TGG 0.514505 5.2:+19477152 None:intergenic
TTCCATTACCTAGATAGTAA+TGG 0.516269 5.2:-19479156 MS.gene027986:intron
TATGGCAAAGATTTAACAGC+CGG 0.518073 5.2:-19483521 MS.gene027986:CDS
TATCGTTCTTGTAATATCAC+AGG 0.519358 5.2:-19476711 MS.gene027986:CDS
TTTGGCCTAGAAATCCCAAT+CGG 0.521150 5.2:+19482594 None:intergenic
AGTTACTTCTGCCGAAGTGT+TGG 0.521693 5.2:-19478117 MS.gene027986:CDS
TCTGCAGGGGCAAGGTCTAT+GGG 0.522236 5.2:-19484819 MS.gene027986:CDS
AATAGCAAAGTGCATGGTAG+AGG 0.523953 5.2:-19483131 MS.gene027986:CDS
TATGTTGTCATGTAAGAAGA+TGG 0.525735 5.2:+19477009 None:intergenic
TCTGATCGTCATAGCAGATC+CGG 0.526463 5.2:-19477806 MS.gene027986:CDS
TCTCCGTCCCTACGTTGTTG+AGG 0.527493 5.2:-19482736 MS.gene027986:CDS
TCAGGTAAGGATTGCTGGAG+AGG 0.527967 5.2:+19482674 None:intergenic
ATTCTTTATTCTTTGGAGAG+TGG 0.532153 5.2:+19483383 None:intergenic
GCCTCTATGGCAGCTATCAA+CGG 0.538256 5.2:+19477972 None:intergenic
TCAACAGGTGTTGCCTCTGA+AGG 0.541002 5.2:-19484905 MS.gene027986:CDS
CATATTCACTGCATTTCTCA+TGG 0.544524 5.2:-19476990 MS.gene027986:CDS
TGGAATTGTTGGTGAAAGTA+GGG 0.547426 5.2:-19483234 MS.gene027986:CDS
GTTTCCACCTGGCACTGTAA+AGG 0.547912 5.2:+19477051 None:intergenic
TCCATACCGATATTTCCAAT+AGG 0.548152 5.2:+19483334 None:intergenic
CATGAGGAGCTAGTGAAAGA+TGG 0.552759 5.2:-19477151 MS.gene027986:CDS
CTGCTATGACGATCAGATGA+AGG 0.554930 5.2:+19477812 None:intergenic
GTTCATTGTAGTACCAATCA+TGG 0.555376 5.2:-19476753 MS.gene027986:CDS
GTGCCACTTGGAAAAGATGT+AGG 0.556314 5.2:+19482617 None:intergenic
CGAGTTCATAAATGCCATGA+GGG 0.557678 5.2:-19478019 MS.gene027986:CDS
TGCGTGAAGTAGCGAAGACC+TGG 0.560154 5.2:+19483285 None:intergenic
GCCTATTGGAAATATCGGTA+TGG 0.560203 5.2:-19483335 MS.gene027986:CDS
ACCTTGTTCATGATCTGCAC+CGG 0.561022 5.2:+19483502 None:intergenic
CCAATGTGGCATAGGCAGAA+AGG 0.561188 5.2:-19479624 MS.gene027986:CDS
CATTTGTTTATTGAACGGAT+GGG 0.564108 5.2:+19476647 None:intergenic
ACTTTCTCAAGAGCTTACCT+TGG 0.568114 5.2:-19476886 MS.gene027986:CDS
TAATAGTTCAAAGCTCAAAG+AGG 0.573010 5.2:-19478088 MS.gene027986:CDS
TCCGCGGTCCAATGTGGCAT+AGG 0.573491 5.2:-19479632 MS.gene027986:CDS
AAGGAAGCCGAAAATGATGA+TGG 0.573881 5.2:-19477205 MS.gene027986:CDS
AGACCAAACAATAACTTCCA+AGG 0.574401 5.2:+19476869 None:intergenic
TTGCAGATTTGAGCGTGGAA+AGG 0.576225 5.2:-19479754 MS.gene027986:intron
CTACTTTGTAAGATATTCCG+TGG 0.578446 5.2:-19479204 MS.gene027986:CDS
AGGGATATGAAACCCATAAG+TGG 0.578644 5.2:-19476775 MS.gene027986:CDS
CAAAACTATCTGCAGGGGCA+AGG 0.578679 5.2:-19484827 MS.gene027986:CDS
TCAGGTTTCCAAGAAGATGA+AGG 0.579247 5.2:-19484762 MS.gene027986:CDS
GAATAAAGAATTGTTCTGGA+AGG 0.580301 5.2:-19483371 MS.gene027986:CDS
GTGGAATTGTTGGTGAAAGT+AGG 0.581941 5.2:-19483235 MS.gene027986:CDS
GTAGTACCAATCATGGCACT+TGG 0.582602 5.2:-19476746 MS.gene027986:CDS
TGCATGGTAGAGGAAATAAA+GGG 0.582858 5.2:-19483121 MS.gene027986:CDS
ATGTTGTCATGTAAGAAGAT+GGG 0.590707 5.2:+19477010 None:intergenic
ATGAAATCTTCCGCTCAACA+TGG 0.593061 5.2:-19476676 MS.gene027986:CDS
ATGATTCCCTCAACAACGTA+GGG 0.595992 5.2:+19482729 None:intergenic
TTGGTGAACTATTCCTTCAG+AGG 0.596647 5.2:+19484892 None:intergenic
ATGTGGCTTTGAGATTACTC+AGG 0.601489 5.2:-19482802 MS.gene027986:CDS
GTTTCCAAGAAGATGAAGGA+TGG 0.602149 5.2:-19484758 MS.gene027986:CDS
TTGGCCTAGAAATCCCAATC+GGG 0.602780 5.2:+19482595 None:intergenic
CATATCATTCAACTATCATG+TGG 0.604069 5.2:-19483614 MS.gene027986:CDS
GCTGCCCCAGATTTGGAGCA+AGG 0.605489 5.2:-19484623 MS.gene027986:intron
ACCACAAAAGATTTCTTCCG+CGG 0.610501 5.2:-19479648 MS.gene027986:CDS
TACCGAAATAGCAAAGTGCA+TGG 0.611483 5.2:-19483137 MS.gene027986:CDS
CTCGAATGTATATATGACAT+CGG 0.616856 5.2:-19483311 MS.gene027986:CDS
ACATTCTGTCAATGGACGGA+AGG 0.617474 5.2:-19477899 MS.gene027986:CDS
GCCGTTGATAGCTGCCATAG+AGG 0.619118 5.2:-19477973 MS.gene027986:CDS
ATTAGGATTAGCATCCCTCA+TGG 0.622934 5.2:+19478005 None:intergenic
TAGTACCAATCATGGCACTT+GGG 0.623895 5.2:-19476745 MS.gene027986:CDS
GTTATGCAAAACTATCTGCA+GGG 0.624004 5.2:-19484833 MS.gene027986:CDS
TTCCCTCAACAACGTAGGGA+CGG 0.626973 5.2:+19482733 None:intergenic
TCATACATTCTGTCAATGGA+CGG 0.635424 5.2:-19477903 MS.gene027986:CDS
GCCTATGCCACATTGGACCG+CGG 0.638415 5.2:+19479631 None:intergenic
TTATGCAAAACTATCTGCAG+GGG 0.646152 5.2:-19484832 MS.gene027986:CDS
AATGATTCCCTCAACAACGT+AGG 0.651538 5.2:+19482728 None:intergenic
CCAACAAATATGCAACTCCA+CGG 0.652186 5.2:+19479187 None:intergenic
AGCAGTGATTGCTGGAAATG+TGG 0.655602 5.2:-19483263 MS.gene027986:CDS
TATACCAATGAAAATCAAGA+CGG 0.658060 5.2:+19476934 None:intergenic
ATTTCTTCCGCGGTCCAATG+TGG 0.663733 5.2:-19479638 MS.gene027986:CDS
ATATGCATGCAAGACGAACA+CGG 0.664176 5.2:-19483199 MS.gene027986:CDS
ATGTTGCATGCATCCCAACA+CGG 0.665878 5.2:-19478048 MS.gene027986:CDS
TTGCATTGCAGATTTGAGCG+TGG 0.668467 5.2:-19479759 MS.gene027986:intron
TTGTTCTGGAAGGTGATGAG+AGG 0.672631 5.2:-19483361 MS.gene027986:CDS
AAAGGCTGCAGCAAACATGA+TGG 0.673254 5.2:+19477069 None:intergenic
CCGACCTTGCTCCAAATCTG+GGG 0.679024 5.2:+19484619 None:intergenic
GCCGGTGCAGATCATGAACA+AGG 0.680709 5.2:-19483503 MS.gene027986:intron
ATGCAACATTGCTTCATACG+CGG 0.681582 5.2:+19478062 None:intergenic
TCGAGTTCATAAATGCCATG+AGG 0.690060 5.2:-19478020 MS.gene027986:CDS
GCAGCCTTTACAGTGCCAGG+TGG 0.698792 5.2:-19477055 MS.gene027986:CDS
GGCTGCAGCAAACATGATGG+TGG 0.726270 5.2:+19477072 None:intergenic
GATATTCATTGAAAGCCATG+AGG 0.729208 5.2:-19477167 MS.gene027986:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.2 gene 19476646 19484936 19476646 ID=MS.gene027986
chr5.2 mRNA 19476646 19484936 19476646 ID=MS.gene027986.t1;Parent=MS.gene027986
chr5.2 exon 19484624 19484936 19484624 ID=MS.gene027986.t1.exon1;Parent=MS.gene027986.t1
chr5.2 CDS 19484624 19484936 19484624 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
chr5.2 exon 19483504 19483719 19483504 ID=MS.gene027986.t1.exon2;Parent=MS.gene027986.t1
chr5.2 CDS 19483504 19483719 19483504 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
chr5.2 exon 19482927 19483418 19482927 ID=MS.gene027986.t1.exon3;Parent=MS.gene027986.t1
chr5.2 CDS 19482927 19483418 19482927 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
chr5.2 exon 19482582 19482824 19482582 ID=MS.gene027986.t1.exon4;Parent=MS.gene027986.t1
chr5.2 CDS 19482582 19482824 19482582 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
chr5.2 exon 19479591 19479770 19479591 ID=MS.gene027986.t1.exon5;Parent=MS.gene027986.t1
chr5.2 CDS 19479591 19479770 19479591 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
chr5.2 exon 19479369 19479428 19479369 ID=MS.gene027986.t1.exon6;Parent=MS.gene027986.t1
chr5.2 CDS 19479369 19479428 19479369 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
chr5.2 exon 19479157 19479231 19479157 ID=MS.gene027986.t1.exon7;Parent=MS.gene027986.t1
chr5.2 CDS 19479157 19479231 19479157 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
chr5.2 exon 19477884 19478195 19477884 ID=MS.gene027986.t1.exon8;Parent=MS.gene027986.t1
chr5.2 CDS 19477884 19478195 19477884 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
chr5.2 exon 19477766 19477845 19477766 ID=MS.gene027986.t1.exon9;Parent=MS.gene027986.t1
chr5.2 CDS 19477766 19477845 19477766 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
chr5.2 exon 19476646 19477257 19476646 ID=MS.gene027986.t1.exon10;Parent=MS.gene027986.t1
chr5.2 CDS 19476646 19477257 19476646 ID=cds.MS.gene027986.t1;Parent=MS.gene027986.t1
Gene Sequence

>MS.gene027986

ATGATGAAATCAACAGGTGTTGCCTCTGAAGGAATAGTTCACCAAGTACTCACTAAAGACAACTATGAAAGATGGAAAATTGTTATGCAAAACTATCTGCAGGGGCAAGGTCTATGGGACGTTGTCATCCAGAATGCTGAATCTCGTACTGATCAGGTTTCCAAGAAGATGAAGGATGGACAGCAAGCTTTACACTTCTTTCAACTATCATCATGTAAACGAGATTTTCTTGAAACAGTCAAAAAATCAGGCTGGAATAACTTCACCAACCAAAATGACCCAGATTCAAGAGCTGCCCCAGATTTGGAGCAAGGTCGGTATCCGTATACTCATGTCGTTTCTGTATTTTTGTAGAACAACTCCTCACTGCGATACTGTTACTCGCTTTCTCTATTGTTTTAGTTTTCATGGCAATATCTAAGTTTATTTGTATATATTTGATTGTTTCATAAGTTTTCTAAAAAATGATTGTTCAAATAACATATTCTATTTTTTTCAATAACGGTATCTAGACACACCCAATTTCTCTACATTCATTCAACAATTCATTTTCTCTATATCTCTCTTCCGAGCACATTGTGAATCTATCACATTCACTCTCTTTTATTCTTCTTACAATCAAAATTAAAATTATTTTTATTTTTTAAAAATATAATTATTATTAATAATAAATATACAATTTTTTTCTTTTGTCCTAACAATTTTTTATAATACTTGCAAATCCAACACATTTTACTTTTAGTTTTGTTGTTTTGATTTAGTCTCTACATAATTTGTTTTATGTTGCAATTGATCCATATAAATTGCTATTAATCTTTATTTTGATGGATTAAAATCAAATTCTTTTCAAATTACATATATAAATCAATTTAATATAAATAAATTTGATAGAAACTTAAACAAAACAACAAGGACGACTTACTTTTTTTAATCAAACCAAGGACTTTAACTTTTAATTAAGGACTTGCTTAAATAAGCCGCAACTTTTAAACTTATTTGAGTCCTTGTGTCTGACTCAAATATTAAAAGGACCAACAACTTGTTATATTGAAGTCGTGTTTAATTAACTTTATATTGCACATCTGGTCCTCGTTGTTACATTTTAAGGGTTTAGCTGTAAGAATATTTTATGTGGAATTACAATTTCAAACATTAATGTGTATATGTTTATGTTTCCCTCTAAATATTTTTGATATTTCCCTCTATGAATATGTTAGGGCAAGGCCTTTTAGGCGTTATCCAGACTGTTTCAGAATTCGAAACTGATGCTTCATGGAAAATAAAGGATGTGAAAGCTTTGCATATCATTCAACTATCATGTGGACGAGAGATTGCTGATGAGATTAGTCATTTTAAAACAGCCAAAAGCGCCTGGAATCATTTAGGCTCATTATATGGCAAAGATTTAACAGCCGGTGCAGATCATGAACAAGGTACCATTAACTGATTTAAAAGTTTCATAAACTGATTTTTATGACAATGAATGCGCCTCATCAAACTCTTGAATGATAATGGCAGATGTGGATGATAGCCACTCTCCAAAGAATAAAGAATTGTTCTGGAAGGTGATGAGAGGTACGCCTATTGGAAATATCGGTATGGACTCGAATGTATATATGACATCGGCTTCCAGGTCTTCGCTACTTCACGCAGCAGTGATTGCTGGAAATGTGGAAAATGTGGAATTGTTGGTGAAAGTAGGGAAAGACACGTTAATATGCATGCAAGACGAACACGGTGACACAGCTCTTGCGCTTGTTGCACGTTATACTGGAAATACCGAAATAGCAAAGTGCATGGTAGAGGAAATAAAGGGACTCCCAGAAACGTTGCTAGAAAAGGAGAACAATGACAATGTAATCCCTATTCTTCTAGCTGCTGCTAATGGACACAAAGAATTGACTTCCTATCTTTACTCCAAAACCCCCACCAAATCCAAAGTGTTTGACAAATCCAATTCACAGAATCGAGTTTTGCTTCTTTCACTATGTATCACTTCTGAAATATTTGGTAAATAAATTTATAGTTTTTGTATGTTGTTAATTTCTTTTTGGTATTTTGATGTAATACTTGTTTTATTCAAATGTGTGGTTTGATTTGATCCATGATCAGATGTGGCTTTGAGATTACTCAGGCGTTTCAAAGATCTGCCCGAGCAATCATTATCAATATATAACTTCTCCGTCCCTACGTTGTTGAGGGAATCATTATCACTACCTAGCAAAATATCAGAATCAGATCCTCTCCAGCAATCCTTACCTGATAAATTCTCAGCCTTAGTTGCATTGGCAAAAATGCCTACATCTTTTCCAAGTGGCACCCGATTGGGATTTCTAGGCCAAATTATTTATGAAAGTAAGTTTTCACACTATTCTTTCTTTATTGGTAATAGTGATAACCAGGCAACATTCCAATACCCTGAAATACATATTGTAAAAGAAAAATAGTTTAAATGAAGTTAGATGGTTAATACATATAACGGCAACAACAAAACCCAATAGATACTATTCAGATTAAACTCAAGATAATATGTCATGTAATTGATTTTGATTGTGTTGATAAACTTATCTCTTTGAGTTAAAACATGTGGGTTCAATGTGGCTGCAATAGAAAAATACATAAAACCTAAGTTAGTAATATGATAATAACGAATTGATGATGGAGATTATGAAAGATGATAGAATAATAAGATACATGTCAAATATTGTAAAGCATGAGCGTCCTCAACACTGAGATTTTCCATGAGCATGTGAGATTGAGGTTCAACTGGTATGCAATGACGTCGCATAAAAATAATGAGCATCAATTAATATAATAAGAAGCGGGTTGTGAGTTTTTACGGTTTTGGTGATGTGGTGCAATTACTGAGATTATGGGTGGGAATTAGGCTTAAGAGGGGATTAGGGTTGAGCATAATTTAAGAGACATTTATAATAATAGAATAGATACATAAATATAATATGATACAATGGTTATGGTAATTTGTACATTTATCGCGTATTAATAAGTTATTTAAAAAGGATATGGAACAAATATAAGAAAAAGGTAACCGAGTGATTCAACTCAAGTGATTAATGAACTTCATCAAATGATGAATTGTTCGGGAGAACAAGTTGGATTTTTGGATGAAACAATTAAAATCTCACAACCAAACTACAGATTACTAGGGGGACTCTTTTTCCTGAGATCTAAAGGATTAACAAAAATAAAAAAAAAGATAAGTAAAGGGATATGTTAGTATATCCATGACGATGATCATTTAAAATTTACCAGTTCTTTATCAACACTAGTGATTAACTTTATATTATTCCTTCCGGTTCTTTTTATAAAAGATAAATGTGTCAACAAAAGTTGATGTATTTAGTTTATAATATTGACTAAATACATCAATTTTCGTTGATCCATTTTTCTCTTATAAAAAAGTACCGAAAGGAAAGTATGTAGATATTTATCCATATAATGATAGTTAAATATTAGATCATAAAAGAAAGAAAGAAACAATGTAATAAAATATTTGAATAGAGGAGGATTGCTATATGCTGCGAAGGAAATGATGCACGACCTGGATACGACAAAACGTGGGCAATGCTTTCACACTTTTGCTGTGAAGGAGTACATCCGGTTGGAAAAAATCAATCTGCATAGCATTACTGGCTGATTTGATTCATTACTGACGGATTCGTTTATTAATGGCAACCGACCATTACCGACGGATAAGCGGATTACTGACGGATTAATTGCTTCAATTACTGGCGGAATTTGAGTATTACCGACGAATTCGAATGCAACATTATTACTGGCGGATATGTTACATTACTGGCGGAATATTTAATCCGTTATACTATTACTGTCGGATTTATATCCCTCAGTAACGCAAAGGCACATTATTGGCGGATCTATTGCATTACTGGCGGAATATTTAATCCGTTATACTATTACTGTCAGACTTATATCCCTCAGTAACGCAAAGGCATGTGCGTTTACATTATTGGCGGAATATTTCCGCAGTAATACATCATAGGTTCTTTATTGATATTACTGGCGGATTTATCCGTCAGTAATTACAGAGGGCCTAAATCCCTCAGTAATGCAATTTTGAATTGGTTAGTAATATTGGGAATTCTTGTAGTGAGACAATGATTTTGATTGTTGTTGGATTAGACCAAAGAGATTGTTAAAGGTGGTGGGGGGAAGATGGAAGTGTCTTGAAAGAAATATAAGAATCTCATGAAAGATTTCTACAAATAGCGTCAACGATTTGAAGTGGACTTGACGACGACAGACAAAGTTGTGTGCATGTGACGGGGAAGAGGAGAGCTCCAAGTGAATAATACGACAAGCAGGGGCGAATCTAGTGTGTACTCAGGGTGTGCACCTGCACACCCTCAACTTTGAAAAATTGCATTAATATCCCTATTCTTTTTGCATTTTGCACACCCTGGGATTTCACTTAAGGTAACCGAGTGATTCAACTCAAGTGATTAATGAACTTCATCAAATGATGAATTGTTCGGGAGAACAAGTTGGATTTTTGGATGAAACAATTAAAATCTCACAACCAAACTACAGATTACTAGGGGGACTCTTTTTCCTGAGATCTAAAGGATTAACAAAAATAAAAAAAAAAGATAAGTAAAGGGATATGTTAGTATATCCATGACGATGATCATTTAAAATTTACCAGTTCTTTATCAACACTAGTGATTAACTTTATATTATTCCTTCCGGTTCTTTTTATAAAAGATAAATGTGTCAACAAAAGTTGATGTATTTAGTTTATAATATTGACTAAATACATCAATTTTCGTTGATCCATTTTTCTCTTATAAAAAAGTACCGAAAGGAAAGTATGTAGATATTTATCCATATAATGATAGTTAAATATTAGATCATAAAAGAAAGAAAGAAACAATGTAATAAAATATTTGAATAGAGGAGGATTGCTATATGCTGCGAAGGAAATGATGCACGACCTGGATATGACAAAACGTGGGCAATGCTTTCACACTTTTAAAATAAATAAAGTAAAACACCACTTAAAGCAAAACTAAAGGGCTGGTGTGCATTCGTCGTTATTCGTCAACTCCTCTCTCGCACCATTTATCTTTCTCCATTTGAATAAACCTCTTTTAAAAAGAAAATATTTGAACATAATTTGCATTGCAGATTTGAGCGTGGAAAGGAGTTTCAACATTGAGTATGATATTGCCGAAATTGGGAACTTCGTCCAAAAGGTAACATGTGTTGCTGATGTCGCTAATGAAAGACCACAAAAGATTTCTTCCGCGGTCCAATGTGGCATAGGCAGAAAGGTTTTGAATTTCGTTGTTCTAATAATGCTTTCGGGTACGCTATGTACTTTCTCTTTAACTATTTCAGTCTTCCAACATCACATATTAATAATAAAGACCTATGCCTTTTAACCGGTACTTTTATGACACAAGTTAGCAAAACCTGTTTTTTATTACCCACATGAATATATATAAATGGCTCAAAGCTTTTATTTAGGTTGGCGCTTCTGCAAACTGTTGATATCGATATTTCTCTTTTGGCCAGTAAAGCTTTTGGGTATGTCGTGTACTGTCTCAGTTTGCAATATCATGCTTTAATCATTTTTTATTTCTTCTCTGATATTTTTTCTTTTTATTATTTTTTTTAAGGATTTCTTTTTACTATTTCTACACTGATTTTGTTGATTTGTTCAGGTCAACTACTTTGTAAGATATTCCGTGGAGTTGCATATTTGTTGGTTCAAGTTTTCCATTACCTAGATAGTAATGGTACGTAAATTCAAATGCAAAAGTTTGATTCATTTCAATAATCATCCAGTAAAAAAAACTCATTAATTATTCCCTCACATTTCAAATATGTGATGAATTAGGGGAGCGGTTAAAAAGTACTACTGATTTTTATACGAAAATTCACGAAGTGCAAATTGATATAAAAAAAATGTGTAATTTCCCCTCGTTTCTTTGAAAAAAGAACTAGATTAAACTAAAAAATAAAAAATAGAAGAAACTTGACATAAAAGCTTTCTCATGCCAATAAGAGTGAAGTCGGCAAAATTTGGAACAAGGGAGTTTAAAATCAATTTCATTATCTTTTGCAAGCTGCTTTGAAAAATCAATTCAAAAAACGTGTTAGCATGGAAGATGCCGACTCATAAATCACATCATGACGAATTTAAAGTTATCTTTTACTCTCAAATTAAAAAGTTGTTTAAATAATGGAAAAAAAATTACTTTGATTCATGAAATTTTGTATATACGATCAAATATTGGTTTCACTAATTAGAAAACTTCAATGTTCCAACCTGCAAAACTGTCTGCAACTCATGCGGAAACCTATATCAGTAGGAAAACATGTAACATCAGCTATCACACGAGTCAATTCGCTTTGCACCAATACTAATAACAATTGATATACTAATTTGCAATAGACACGAATACAATTATACACTATAAACCATACACCAGTCAACAAATCGATCGAACCGCAAAATATTGAACCATATTTAAAACATAATCCCTCCTACAATATAATTACTAATATAGTTGGAACGTCAACAGATCATAGTGATGTCTTAGCATTTAAAATATTGGTTAAAAAAAATCATTATGATTGATAGTTTAGAAAAAATCTTGATATTGATGTTGCATGTAAAGCTTTCCTTATATGCTTAATTCGAATTGATTTTTTTTAACAACTCCAAGACTGATTAGGTCCCTTCAATTATGTCAGGAATCAGGAAGATATATGATCAGAAATACACACATTATGAAGTTATAGGAATATTGAGTTACTTCTGCCGAAGTGTTGGAAAATTTAATAGTTCAAAGCTCAAAGAGGCTTCCGCGTATGAAGCAATGTTGCATGCATCCCAACACGGAATAATCGAGTTCATAAATGCCATGAGGGATGCTAATCCTAATTTTCTTTCAGCCGTTGATAGCTGCCATAGAGGCATATTTTCGTATGCAATCTTGCATCGCAAACAAAATGTGTTTCAACTCATACATTCTGTCAATGGACGGAAGGAGATACTTAGACATCGTATAGACTCGTTTGGCATAATTTGTTGCACCTGGCAGCACATTTAGGTCCTTCATCTGATCGTCATAGCAGATCCGGTGCTGCTCTGCAAATGCAAAGAGAGATTCAATGGTTTAAGGTCACCCATCTAAATTAATTGTCTTAAATATACAATTGATTGAAGATACATATTCCATATTTTAATTCAATATTAGAAGTGCAAAATAATCAACGTATACTAGCTTTGTTGGCCATTTTACAAAACATGGTATAGGAAAATCCTTTTAGATTACATGTTATAAATACATATTAAGCATTTCCTAATAAATGTTGTTGAAACACCATATGTTCAGTACTCTAAAATTACACTCCAACAGTATTAGGTACGAAACACCCCTTCTGTTACGAAATATAAGTAAAAATGAGTTAGTTTTTTTGGACCAAATACATCAAACTTTCGTTAACCAACTTTTGCTTATATTTCGTTACAGATGTTGTTACAAAGCCTCCAAATGAACCAAGATTCATGTGTTTCAACATTGTGATTTTAAAGAAGCTTCAAAACCACATAATTCCACCAATCTATTTAATTTACAGTATATGCATGTCTCTTGCTTCTTTATAAGTTGTTTATGATACCACTGCAGGCGGTTGAGAAAGTTTTACATCCCAAGTTTAAGGAAGCCGAAAATGATGATGGTAAGAAGCCTTATGAGATATTCATTGAAAGCCATGAGGAGCTAGTGAAAGATGGAGAGAAATGGGCAAAAGACACAGCAACATCTTATACAATTGTAGGTACTCTCATCACCACCATCATGTTTGCTGCAGCCTTTACAGTGCCAGGTGGAAACGACGACAAAACTGGTTTGCCCATCTTCTTACATGACAACATATTCACTGCATTTCTCATGGCAGACGCAGTATCTCTCTTCGCATCTGCGACTTCCGTCTTGATTTTCATTGGTATACTCACATCGCGTTATGCTGAGACAGACTTTCTCAAGAGCTTACCTTGGAAGTTATTGTTTGGTCTTTTGCTCCTTTTTCTCTCTGTTTGTTCTATGATAGTTGCATTTAGCGCTGCCATTATCGACATGATCTTAAAGGGATATGAAACCCATAAGTGGTTCATTGTAGTACCAATCATGGCACTTGGGAGTATTCCGATTATCGTTCTTGTAATATCACAGGTGAGCTTCATGTATGAAATCTTCCGCTCAACATGGAAAAATCCCATCCGTTCAATAAACAAATGA
Protein sequence

>MS.gene027986.t1

MMKSTGVASEGIVHQVLTKDNYERWKIVMQNYLQGQGLWDVVIQNAESRTDQVSKKMKDGQQALHFFQLSSCKRDFLETVKKSGWNNFTNQNDPDSRAAPDLEQGQGLLGVIQTVSEFETDASWKIKDVKALHIIQLSCGREIADEISHFKTAKSAWNHLGSLYGKDLTAGADHEQDVDDSHSPKNKELFWKVMRGTPIGNIGMDSNVYMTSASRSSLLHAAVIAGNVENVELLVKVGKDTLICMQDEHGDTALALVARYTGNTEIAKCMVEEIKGLPETLLEKENNDNVIPILLAAANGHKELTSYLYSKTPTKSKVFDKSNSQNRVLLLSLCITSEIFDVALRLLRRFKDLPEQSLSIYNFSVPTLLRESLSLPSKISESDPLQQSLPDKFSALVALAKMPTSFPSGTRLGFLGQIIYENLSVERSFNIEYDIAEIGNFVQKVTCVADVANERPQKISSAVQCGIGRKVLNFVVLIMLSGWRFCKLLISIFLFWPVKLLGQLLCKIFRGVAYLLVQVFHYLDSNGIRKIYDQKYTHYEVIGILSYFCRSVGKFNSSKLKEASAYEAMLHASQHGIIEFINAMRDANPNFLSAVDSCHRGIFSYAILHRKQNVFQLIHSVNGRKEILRHPHLGPSSDRHSRSGAALQMQREIQWFKAVEKVLHPKFKEAENDDGKKPYEIFIESHEELVKDGEKWAKDTATSYTIVGTLITTIMFAAAFTVPGGNDDKTGLPIFLHDNIFTAFLMADAVSLFASATSVLIFIGILTSRYAETDFLKSLPWKLLFGLLLLFLSVCSMIVAFSAAIIDMILKGYETHKWFIVVPIMALGSIPIIVLVISQVSFMYEIFRSTWKNPIRSINK