AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027995

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027995.t1 MTR_5g028190 70.230 608 103 4 123 727 1 533 0.0 835
MS.gene027995.t1 MTR_5g028130 71.233 365 76 3 368 729 1 339 2.83e-177 510
MS.gene027995.t1 MTR_5g028930 41.176 697 297 19 61 726 2 616 1.43e-137 419
MS.gene027995.t1 MTR_5g028450 61.059 321 94 3 409 725 1 294 1.30e-122 369
MS.gene027995.t1 MTR_5g028110 70.984 193 38 1 537 729 2 176 3.00e-87 272
MS.gene027995.t1 MTR_5g091220 32.171 631 359 16 104 725 31 601 4.05e-76 257
MS.gene027995.t1 MTR_2g438740 30.380 632 329 20 103 727 41 568 5.81e-74 251
MS.gene027995.t1 MTR_6g017215 58.696 184 76 0 537 720 6 189 5.69e-65 216
MS.gene027995.t1 MTR_5g028510 42.342 333 141 8 24 316 6 327 1.31e-63 215
MS.gene027995.t1 MTR_3g455790 28.852 610 366 13 104 708 33 579 1.96e-63 222
MS.gene027995.t1 MTR_3g055310 29.301 529 304 12 199 721 12 476 4.29e-62 216
MS.gene027995.t1 MTR_5g028690 50.235 213 98 3 519 726 26 235 8.51e-62 207
MS.gene027995.t1 MTR_3g055310 30.938 501 277 12 226 721 38 474 9.15e-62 215
MS.gene027995.t1 MTR_3g055310 30.502 518 290 13 210 721 48 501 1.39e-61 215
MS.gene027995.t1 MTR_3g055310 30.998 471 263 10 252 721 39 448 2.86e-60 210
MS.gene027995.t1 MTR_3g055310 31.291 457 252 10 266 721 1 396 1.32e-59 207
MS.gene027995.t1 MTR_2g438700 28.502 621 348 14 107 724 161 688 3.45e-57 207
MS.gene027995.t1 MTR_2g438720 26.225 755 396 21 33 725 36 691 5.21e-57 207
MS.gene027995.t1 MTR_3g055310 37.143 315 162 4 407 721 55 333 5.39e-57 198
MS.gene027995.t1 MTR_2g438640 25.524 764 421 20 17 725 20 690 6.07e-56 204
MS.gene027995.t1 MTR_3g452020 27.360 625 383 15 104 719 33 595 4.06e-53 194
MS.gene027995.t1 MTR_2g438440 35.849 318 175 4 409 725 396 685 5.48e-52 192
MS.gene027995.t1 MTR_2g438640 24.738 764 401 20 17 725 20 664 1.99e-50 187
MS.gene027995.t1 MTR_2g438400 34.375 320 177 5 409 725 255 544 3.27e-49 182
MS.gene027995.t1 MTR_2g438760 32.075 318 187 4 410 725 271 561 4.96e-45 170
MS.gene027995.t1 MTR_2g438760 26.054 261 167 9 53 311 5 241 1.21e-13 74.7
MS.gene027995.t1 MTR_2g020510 25.287 696 384 20 102 707 4 653 2.00e-43 167
MS.gene027995.t1 MTR_5g028900 51.613 155 63 2 539 693 4 146 1.49e-42 152
MS.gene027995.t1 MTR_5g028840 45.699 186 92 1 537 722 2 178 8.21e-42 151
MS.gene027995.t1 MTR_3g452020 28.027 446 264 10 264 707 13 403 3.02e-40 154
MS.gene027995.t1 MTR_5g028830 38.672 256 127 7 284 513 39 290 3.02e-40 150
MS.gene027995.t1 MTR_2g017930 25.797 659 371 21 139 728 37 646 1.56e-37 149
MS.gene027995.t1 MTR_3g058570 31.532 333 178 7 408 726 83 379 1.48e-35 139
MS.gene027995.t1 MTR_2g020450 31.529 314 185 5 409 721 393 677 1.56e-32 134
MS.gene027995.t1 MTR_2g020450 28.846 208 131 7 102 303 4 200 1.58e-12 71.2
MS.gene027995.t1 MTR_2g020470 31.773 299 149 6 409 707 392 635 8.11e-32 132
MS.gene027995.t1 MTR_2g020470 26.484 219 140 8 102 312 4 209 6.78e-11 66.2
MS.gene027995.t1 MTR_2g438640 37.895 190 116 2 537 725 399 587 1.78e-30 127
MS.gene027995.t1 MTR_5g028920 41.615 161 90 2 369 529 86 242 7.29e-30 119
MS.gene027995.t1 MTR_3g050500 27.545 334 206 8 396 721 414 719 1.62e-28 122
MS.gene027995.t1 MTR_3g051060 27.358 318 201 6 410 723 435 726 1.88e-27 119
MS.gene027995.t1 MTR_3g051060 29.108 213 126 7 107 304 28 230 6.70e-14 75.9
MS.gene027995.t1 MTR_5g085360 26.849 365 195 10 396 718 388 722 7.61e-27 117
MS.gene027995.t1 MTR_5g085350 29.338 317 193 7 409 721 424 713 2.58e-25 112
MS.gene027995.t1 MTR_5g085350 29.338 317 193 7 409 721 437 726 2.77e-25 112
MS.gene027995.t1 MTR_5g085340 28.349 321 199 6 409 724 443 737 1.32e-24 110
MS.gene027995.t1 MTR_3g055750 25.060 415 242 14 104 513 33 383 2.23e-20 94.4
MS.gene027995.t1 MTR_1g113820 30.328 244 131 7 408 646 275 484 5.88e-19 91.7
MS.gene027995.t1 MTR_2g438470 34.375 160 77 3 454 613 366 497 9.91e-18 87.4
MS.gene027995.t1 MTR_1g113730 26.104 249 146 6 381 627 23 235 2.31e-14 75.9
MS.gene027995.t1 MTR_3g058550 31.667 180 100 6 139 304 9 179 3.40e-13 70.1
MS.gene027995.t1 MTR_6g016185 28.251 223 119 9 437 659 16 197 2.36e-11 63.9
MS.gene027995.t1 MTR_2g438580 39.474 76 45 1 551 626 2 76 8.21e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027995.t1 AT3G18670 35.580 638 354 15 104 729 50 642 1.63e-120 375
MS.gene027995.t1 AT3G18670 33.542 638 312 20 104 729 50 587 1.59e-93 303
MS.gene027995.t1 AT5G04690 30.915 634 380 12 95 725 67 645 4.05e-81 272
MS.gene027995.t1 AT5G04700 30.573 628 379 12 103 725 80 655 6.04e-79 266
MS.gene027995.t1 AT5G04690 30.394 635 368 14 95 725 67 631 3.46e-76 258
MS.gene027995.t1 AT5G04690 30.709 635 346 13 95 725 67 611 1.34e-74 253
MS.gene027995.t1 AT5G04680 29.376 657 386 18 103 725 67 679 1.58e-68 238
MS.gene027995.t1 AT5G04680 29.467 638 381 16 103 725 67 650 3.10e-68 237
MS.gene027995.t1 AT5G04730 29.983 587 343 12 151 725 58 588 5.74e-67 232
MS.gene027995.t1 AT5G04680 28.683 638 372 16 103 725 67 636 4.98e-65 228
MS.gene027995.t1 AT5G04680 30.329 577 331 16 164 725 7 527 9.51e-65 224
MS.gene027995.t1 AT5G35810 34.756 328 180 5 409 729 35 335 2.86e-56 196
MS.gene027995.t1 AT3G54065 40.102 197 87 2 498 688 8 179 3.14e-40 148
MS.gene027995.t1 AT5G04700 26.442 416 276 8 103 515 80 468 3.10e-35 140
MS.gene027995.t1 AT5G35830 31.737 167 101 5 99 262 81 237 2.61e-13 71.6

Find 163 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCATGCACTTCTCTTTC+AGG 0.124188 5.2:+19300057 None:intergenic
TTGCACTTGGCAGGATATTT+AGG 0.127030 5.2:-19298978 MS.gene027995:CDS
CCAAAGAATTCAAGAGTTTA+AGG 0.157617 5.2:-19299255 MS.gene027995:CDS
AACTTACCCAACTGCTTTAC+TGG 0.201955 5.2:+19299812 None:intergenic
TGTATCACTGCAGAAATATT+TGG 0.219312 5.2:-19301007 MS.gene027995:intron
TTCACTTGCTATGCTCTTAA+AGG 0.220345 5.2:-19297970 MS.gene027995:CDS
CCTTAAACTCTTGAATTCTT+TGG 0.238324 5.2:+19299255 None:intergenic
TAAAGAAGCCTGGAATAAAT+TGG 0.241875 5.2:-19301825 MS.gene027995:CDS
ATACATGATGCAACTGTAAA+TGG 0.242921 5.2:-19299050 MS.gene027995:CDS
TTAGAACGCGCACCAATTTC+AGG 0.251761 5.2:+19301953 None:intergenic
CTTCGCTATATTCGTGTTTC+CGG 0.254247 5.2:+19301225 None:intergenic
AATATATTCCACATCCTCTA+TGG 0.260429 5.2:-19301386 MS.gene027995:CDS
GTGACCTCTATTCCTGAAAT+TGG 0.292763 5.2:-19301965 MS.gene027995:CDS
TGCACTTGGCAGGATATTTA+GGG 0.308043 5.2:-19298977 MS.gene027995:CDS
TTTCCAAGTGGTAGTGGATA+TGG 0.311953 5.2:-19300792 MS.gene027995:CDS
ACTTCGGTCCTTCTCTTCAT+TGG 0.319322 5.2:-19298110 MS.gene027995:CDS
AATTGTATCCATCAGGAATA+AGG 0.321064 5.2:-19299337 MS.gene027995:intron
CTTCACGTTGCGGTAGTTGC+TGG 0.322177 5.2:-19301352 MS.gene027995:CDS
CTAAGACTGAATATGAGATT+TGG 0.338368 5.2:-19301934 MS.gene027995:CDS
AGGGTAATCCTGACCTCTTA+TGG 0.352153 5.2:-19299145 MS.gene027995:CDS
ATTGTGAGTCTTGCAAATAT+TGG 0.353381 5.2:-19300024 MS.gene027995:CDS
CCAAAGACAACTACGAAAAC+TGG 0.369243 5.2:-19302036 MS.gene027995:CDS
CGAGGAAATTGTGAAGAAAT+TGG 0.372095 5.2:-19301326 MS.gene027995:CDS
ATGCAAACTCGTTTATTAAG+AGG 0.378242 5.2:-19301420 MS.gene027995:CDS
AACAGGGATTTCACCATCAT+TGG 0.379563 5.2:+19301144 None:intergenic
TGTTCTTCTTGCTGCTGCTA+AGG 0.379583 5.2:-19301125 MS.gene027995:CDS
TAGTGAAAGCTGGGGAGAAA+TGG 0.384321 5.2:-19298313 MS.gene027995:CDS
GATAAGTAATAAGTTTCAAT+TGG 0.388393 5.2:+19297836 None:intergenic
AGTTGCACCCAATTTATTCC+AGG 0.399506 5.2:+19301817 None:intergenic
GCTGCAGCTTTCACTGTCCC+TGG 0.408383 5.2:-19298230 MS.gene027995:CDS
TTAGCAGCAGCAAGAAGAAC+AGG 0.410668 5.2:+19301127 None:intergenic
TAGAAACCACAGAGAAGAAA+AGG 0.416389 5.2:+19298012 None:intergenic
TTCCAAGTGGTAGTGGATAT+GGG 0.416844 5.2:-19300791 MS.gene027995:CDS
AAATGCCGTGTAGACGCATT+TGG 0.419337 5.2:-19299011 MS.gene027995:CDS
AATTCCATGTTGCACTTGGC+AGG 0.419647 5.2:-19298987 MS.gene027995:CDS
ATCAGAACTTAGAGAGGCTT+CGG 0.421195 5.2:-19299231 MS.gene027995:CDS
GGGTAATCCTGACCTCTTAT+GGG 0.423361 5.2:-19299144 MS.gene027995:CDS
CTAATATGAGTAAGATTCTC+TGG 0.424630 5.2:+19301863 None:intergenic
TCACTTGCTATGCTCTTAAA+GGG 0.427681 5.2:-19297969 MS.gene027995:CDS
CATTGGTCTTCATAGATAAC+AGG 0.427879 5.2:+19301161 None:intergenic
ACCTTAGTGGCAATGACTTA+AGG 0.429666 5.2:+19298054 None:intergenic
AGATGCAAAGAGAAATTCTA+TGG 0.430747 5.2:-19298914 MS.gene027995:CDS
TTCACGTTGCGGTAGTTGCT+GGG 0.432176 5.2:-19301351 MS.gene027995:CDS
TCTCTTTGCATCTGCATAGC+AGG 0.438079 5.2:+19298924 None:intergenic
GAGATTTGGAACAGGAAGAA+TGG 0.441267 5.2:-19301920 MS.gene027995:CDS
CCACAAATCACACTTTGAAT+CGG 0.443086 5.2:-19300859 MS.gene027995:CDS
TCTGTGTTTCCAAGTGGTAG+TGG 0.443413 5.2:-19300798 MS.gene027995:CDS
CTGAATATGAGATTTGGAAC+AGG 0.444534 5.2:-19301928 MS.gene027995:CDS
GTTCTTCTTGCTGCTGCTAA+GGG 0.445513 5.2:-19301124 MS.gene027995:CDS
GACGGAACTAGAACGATAAC+AGG 0.453839 5.2:+19297903 None:intergenic
CACAAGGAGCTAGTGAAAGC+TGG 0.455549 5.2:-19298323 MS.gene027995:CDS
CGATTCAAAGTGTGATTTGT+GGG 0.456154 5.2:+19300860 None:intergenic
TATAGCGAAGTGTATGACTA+TGG 0.458675 5.2:-19301212 MS.gene027995:CDS
TCCTTAAGTCATTGCCACTA+AGG 0.460081 5.2:-19298055 MS.gene027995:CDS
TCTTGTGGGACATGGTCTGT+GGG 0.464135 5.2:-19301993 MS.gene027995:CDS
TCAATGGCCCATAAGAGGTC+AGG 0.465739 5.2:+19299137 None:intergenic
TTTCAGCGTAACGAGCCGTT+AGG 0.467061 5.2:+19298084 None:intergenic
GTTTATAGATGCAATGAGAA+AGG 0.467103 5.2:-19299165 MS.gene027995:CDS
TTGTGGGCAAGAGGCATTTG+AGG 0.467341 5.2:+19300876 None:intergenic
CTTGTTAGAAACTATCTTGT+GGG 0.467922 5.2:-19302007 MS.gene027995:CDS
ATGCGTGCCAATGCGTATAA+TGG 0.471182 5.2:+19300825 None:intergenic
TGTGCAGCGATTTATATTGA+AGG 0.474499 5.2:+19301470 None:intergenic
TTCATATTGCTAAAGAAGCC+TGG 0.477292 5.2:-19301835 MS.gene027995:CDS
ACAAGGAGCTAGTGAAAGCT+GGG 0.477954 5.2:-19298322 MS.gene027995:CDS
AAAGAAGCCTGGAATAAATT+GGG 0.481257 5.2:-19301824 MS.gene027995:intron
AGCTGGTTCATTTACTCTTG+TGG 0.482801 5.2:-19298279 MS.gene027995:CDS
AGATTTGGAACAGGAAGAAT+GGG 0.485550 5.2:-19301919 MS.gene027995:CDS
TCAGCTCAGCAACAAGAGCT+AGG 0.486371 5.2:+19301248 None:intergenic
CATAGCAAGTGAAGCACAGA+AGG 0.486804 5.2:+19297980 None:intergenic
ATCTTGTGGGACATGGTCTG+TGG 0.486835 5.2:-19301994 MS.gene027995:CDS
AGTGAAAGCTGGGGAGAAAT+GGG 0.487029 5.2:-19298312 MS.gene027995:CDS
GTGAAGAAATTGGTGAAGGA+AGG 0.488215 5.2:-19301316 MS.gene027995:CDS
AATTGTGCACCCCAAGTGTA+AGG 0.491201 5.2:-19298396 MS.gene027995:CDS
TTGCATCTGCATAGCAGGAC+CGG 0.495069 5.2:+19298929 None:intergenic
ATATATTCCACATCCTCTAT+GGG 0.496055 5.2:-19301385 MS.gene027995:CDS
TTGTCTTGATTGTTTCCACC+AGG 0.496117 5.2:+19298212 None:intergenic
TTGTACACCGTTGGAAGACC+TGG 0.502955 5.2:-19301071 MS.gene027995:CDS
TTTATAGATGCAATGAGAAA+GGG 0.503030 5.2:-19299164 MS.gene027995:CDS
ATCATGTATGAGTTCGAAAA+CGG 0.503307 5.2:+19299064 None:intergenic
TGCGATAATTATCATCGGCC+AGG 0.509460 5.2:+19301053 None:intergenic
TGGCAATTCCATGTTGCACT+TGG 0.511014 5.2:-19298991 MS.gene027995:CDS
TTGTGAAGCAGTAGAATGAC+AGG 0.514230 5.2:-19302100 None:intergenic
CAATTCAAAGCTTCTTTCGT+AGG 0.514295 5.2:-19299988 MS.gene027995:intron
TCTTTACCGTTGTACACCGT+TGG 0.515694 5.2:-19301080 MS.gene027995:CDS
TCTGATCTTGATCGTAGATC+CGG 0.515841 5.2:-19298948 MS.gene027995:CDS
CGCACCAATTTCAGGAATAG+AGG 0.521001 5.2:+19301961 None:intergenic
CATGCATGCAGCTTATATGT+TGG 0.523334 5.2:-19298838 MS.gene027995:CDS
CTTTAGGAAGGTTATGTGGA+CGG 0.525505 5.2:-19299850 MS.gene027995:intron
GCTCTTGTTGCTGAGCTGAC+CGG 0.526277 5.2:-19301244 MS.gene027995:CDS
CCGATTCAAAGTGTGATTTG+TGG 0.526820 5.2:+19300859 None:intergenic
TGCAAACTCGTTTATTAAGA+GGG 0.527286 5.2:-19301419 MS.gene027995:CDS
TGTGATGTCGTGCAAGAACA+AGG 0.535047 5.2:+19298181 None:intergenic
GAGGCATTTGAGGAAATCGT+TGG 0.537554 5.2:+19300886 None:intergenic
GGTCCTCCCCATAGAGGATG+TGG 0.539677 5.2:+19301378 None:intergenic
AAGAGAAATTCTATGGTTCA+AGG 0.541072 5.2:-19298907 MS.gene027995:CDS
TCGTAGTTGTCTTTGGTCAG+TGG 0.542773 5.2:+19302043 None:intergenic
TGTGCAACCATTATACGCAT+TGG 0.543058 5.2:-19300832 MS.gene027995:CDS
AGTGCGAAAAGTAACCTTAG+TGG 0.548121 5.2:+19298041 None:intergenic
ATGTTGCAAGCAGCAAAGCA+TGG 0.548691 5.2:-19299197 MS.gene027995:CDS
CAGCTTTCACTAGCTCCTTG+TGG 0.553008 5.2:+19298324 None:intergenic
TTATCGCCAGTAAAGCAGTT+GGG 0.556264 5.2:-19299818 MS.gene027995:intron
AACCCATATCCACTACCACT+TGG 0.557493 5.2:+19300789 None:intergenic
AAGTGTGATTTGTGGGCAAG+AGG 0.558162 5.2:+19300867 None:intergenic
TCTTGTTAGAAACTATCTTG+TGG 0.559427 5.2:-19302008 MS.gene027995:CDS
AGAAACTATCTTGTGGGACA+TGG 0.562860 5.2:-19302001 MS.gene027995:CDS
AGAAACCACAGAGAAGAAAA+GGG 0.563826 5.2:+19298013 None:intergenic
TGTTGCAAGCAGCAAAGCAT+GGG 0.564523 5.2:-19299196 MS.gene027995:CDS
GCTTTAATTTGCAGGTGTGG+TGG 0.564757 5.2:-19301503 MS.gene027995:intron
ACTTGAAAATCTCAAGGAAG+AGG 0.567742 5.2:+19297871 None:intergenic
TAAGGAATCAGAACTTAGAG+AGG 0.568613 5.2:-19299237 MS.gene027995:CDS
GTTTCTTTAGGAAGGTTATG+TGG 0.570625 5.2:-19299854 MS.gene027995:intron
GCCGATGATAATTATCGCAA+TGG 0.574637 5.2:-19301049 MS.gene027995:CDS
TGAAGAAATTGGTGAAGGAA+GGG 0.574748 5.2:-19301315 MS.gene027995:CDS
TTTATCGCCAGTAAAGCAGT+TGG 0.576962 5.2:-19299819 MS.gene027995:CDS
CAGCTCAGCAACAAGAGCTA+GGG 0.580236 5.2:+19301249 None:intergenic
AAATGGGCAAAAGATACAGC+TGG 0.580445 5.2:-19298296 MS.gene027995:CDS
TTATCTATGAAGACCAATGA+TGG 0.582929 5.2:-19301157 MS.gene027995:CDS
TAAGAAAATATCACGGTCAG+AGG 0.584733 5.2:-19301185 MS.gene027995:CDS
TGCGGTAGTTGCTGGGCACG+AGG 0.589759 5.2:-19301344 MS.gene027995:CDS
TCTCTTCATTGGAATCCTAA+CGG 0.591072 5.2:-19298099 MS.gene027995:CDS
CTGTAGACTTGAAAATCTCA+AGG 0.594097 5.2:+19297865 None:intergenic
TCTTGCAAATATTGGCAAAG+AGG 0.597396 5.2:-19300016 MS.gene027995:CDS
GCTTATATGTTGGAACAAAG+AGG 0.600558 5.2:-19298828 MS.gene027995:intron
GCAGCTTTCACTGTCCCTGG+TGG 0.601445 5.2:-19298227 MS.gene027995:CDS
GGAACAAGCATTGCAGCGAG+TGG 0.605832 5.2:-19302079 MS.gene027995:CDS
AGGAAGAGGCGCAATTGTGA+CGG 0.607133 5.2:+19297885 None:intergenic
AGTATATTGAGAGCGGTGAG+TGG 0.609550 5.2:-19301447 MS.gene027995:CDS
ACCATTGCGATAATTATCAT+CGG 0.609954 5.2:+19301048 None:intergenic
TAGAACGATAACAGGAACAC+TGG 0.611619 5.2:+19297911 None:intergenic
AACTACCGCAACGTGAAGAA+CGG 0.612033 5.2:+19301357 None:intergenic
AATTGCCAAATGCGTCTACA+CGG 0.612652 5.2:+19299006 None:intergenic
TTAGGATTCCAATGAAGAGA+AGG 0.614556 5.2:+19298102 None:intergenic
ATGCCTTCTGTGTTTCCAAG+TGG 0.615562 5.2:-19300804 MS.gene027995:CDS
TGCAACTGTAAATGGACGGA+AGG 0.618113 5.2:-19299042 MS.gene027995:CDS
CTCACGAGGCTTCTTATCGT+CGG 0.618144 5.2:+19298361 None:intergenic
CTTGTGGGACATGGTCTGTG+GGG 0.619674 5.2:-19301992 MS.gene027995:CDS
CTACCACTTGGAAACACAGA+AGG 0.622603 5.2:+19300801 None:intergenic
AAGAACGGTCCTCCCCATAG+AGG 0.622685 5.2:+19301372 None:intergenic
AATTGTGAAGAAATTGGTGA+AGG 0.625256 5.2:-19301320 MS.gene027995:CDS
ATATCCTGCCAAGTGCAACA+TGG 0.627952 5.2:+19298983 None:intergenic
GGTGGAAACAATCAAGACAA+TGG 0.629811 5.2:-19298209 MS.gene027995:CDS
ATGATGCAACTGTAAATGGA+CGG 0.638038 5.2:-19299046 MS.gene027995:CDS
CATATCATTCAACTAACATG+TGG 0.643061 5.2:-19301887 MS.gene027995:CDS
TGTCTTGATTGTTTCCACCA+GGG 0.652150 5.2:+19298213 None:intergenic
GAGGACCGTTCTTCACGTTG+CGG 0.655029 5.2:-19301362 MS.gene027995:CDS
CTTTCTGTAAATAACTCACG+AGG 0.657348 5.2:+19298347 None:intergenic
GTGATGTCGTGCAAGAACAA+GGG 0.659099 5.2:+19298182 None:intergenic
ATTCCACATCCTCTATGGGG+AGG 0.660094 5.2:-19301381 MS.gene027995:CDS
TAGCAGCAGCAAGAAGAACA+GGG 0.665135 5.2:+19301128 None:intergenic
TTGGAACAGGAAGAATGGGA+AGG 0.668429 5.2:-19301915 MS.gene027995:CDS
TATTTCAAGAGCTACACGGT+AGG 0.669157 5.2:+19299292 None:intergenic
GTGAAAATGAAAGACAATCG+AGG 0.669964 5.2:-19301280 MS.gene027995:CDS
TCATCGGCCAGGTCTTCCAA+CGG 0.671668 5.2:+19301064 None:intergenic
CATGTATGTATGCGAGTGAG+TGG 0.673783 5.2:+19298857 None:intergenic
TGGTATATAAGAAAATATCA+CGG 0.677081 5.2:-19301192 MS.gene027995:CDS
CTACGATCAAGATCAGAGGA+AGG 0.678354 5.2:+19298954 None:intergenic
CTGCACAAGTATATTGAGAG+CGG 0.680709 5.2:-19301454 MS.gene027995:CDS
GGATCTACGATCAAGATCAG+AGG 0.680818 5.2:+19298950 None:intergenic
TCAATATTTCAAGAGCTACA+CGG 0.684421 5.2:+19299288 None:intergenic
GGTCTTCCAACGGTGTACAA+CGG 0.687225 5.2:+19301074 None:intergenic
TATATTCCACATCCTCTATG+GGG 0.688060 5.2:-19301384 MS.gene027995:CDS
CAAGGAGCTAGTGAAAGCTG+GGG 0.701952 5.2:-19298321 MS.gene027995:CDS
GTTATTTACAGAAAGCCACA+AGG 0.702046 5.2:-19298339 MS.gene027995:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTTATAAAAGAAATTTAA+AGG - chr5.2:19299387-19299406 MS.gene027995:intron 10.0%
!! CTTAAATACTTATAATATTA+GGG - chr5.2:19298348-19298367 MS.gene027995:CDS 10.0%
!! TCTTAAATACTTATAATATT+AGG - chr5.2:19298347-19298366 MS.gene027995:CDS 10.0%
!! AAACTCCTTTAATATATTAT+TGG + chr5.2:19299528-19299547 None:intergenic 15.0%
!! ATTTAAAGGTGATTTATAAA+AGG - chr5.2:19299401-19299420 MS.gene027995:intron 15.0%
!! ATTTAATTAATTAATCACAG+AGG + chr5.2:19300563-19300582 None:intergenic 15.0%
!! TACTTCCAATAATATATTAA+AGG - chr5.2:19299520-19299539 MS.gene027995:intron 15.0%
!! TTTAAAGGTGATTTATAAAA+GGG - chr5.2:19299402-19299421 MS.gene027995:intron 15.0%
!! TTTAATTAATTAATCACAGA+GGG + chr5.2:19300562-19300581 None:intergenic 15.0%
!!! AAAAACTAAATTTGAAATTC+TGG - chr5.2:19301318-19301337 MS.gene027995:CDS 15.0%
!!! ACTGAATTTTAATTAAATTG+AGG - chr5.2:19298961-19298980 MS.gene027995:CDS 15.0%
!!! CAAAAGTTTGTATTTTATAA+AGG + chr5.2:19299444-19299463 None:intergenic 15.0%
!!! CTGAATTTTAATTAAATTGA+GGG - chr5.2:19298962-19298981 MS.gene027995:CDS 15.0%
!!! TTATTAATCCTTGTTTAAAT+AGG - chr5.2:19300441-19300460 MS.gene027995:intron 15.0%
!! AAACTATGTCTAATATGTAT+AGG + chr5.2:19298250-19298269 None:intergenic 20.0%
!! AACTATGTCTAATATGTATA+GGG + chr5.2:19298249-19298268 None:intergenic 20.0%
!! ATGAATAAGATAATAATGTG+TGG + chr5.2:19301439-19301458 None:intergenic 20.0%
!! CAATTAGTAAGGAAAATAAA+TGG - chr5.2:19298373-19298392 MS.gene027995:CDS 20.0%
!! CTAATATACCTATTTAAACA+AGG + chr5.2:19300452-19300471 None:intergenic 20.0%
!! TACACATTAATCTATTCTTT+GGG - chr5.2:19298207-19298226 MS.gene027995:CDS 20.0%
!! TAGTTTCTAAATGTATTGAA+AGG - chr5.2:19298264-19298283 MS.gene027995:CDS 20.0%
!! TGGTATATAAGAAAATATCA+CGG - chr5.2:19298744-19298763 MS.gene027995:intron 20.0%
!! TTAATTAATTAATCACAGAG+GGG + chr5.2:19300561-19300580 None:intergenic 20.0%
!!! ATACATGACTTTTAAATAAG+TGG - chr5.2:19301381-19301400 MS.gene027995:CDS 20.0%
!!! CATTTATTTTCCTTACTAAT+TGG + chr5.2:19298375-19298394 None:intergenic 20.0%
!!! TCAAATTTAGTTTTTGAAGT+TGG + chr5.2:19301313-19301332 None:intergenic 20.0%
! AAAGTCATGTATCTCAAAAT+TGG + chr5.2:19301373-19301392 None:intergenic 25.0%
! AATTTGAAAAGTAAGCGAAA+TGG + chr5.2:19299227-19299246 None:intergenic 25.0%
! ATCAACACACATATATAACA+GGG + chr5.2:19300320-19300339 None:intergenic 25.0%
! CTGAAGATTATTAATCTTCT+CGG - chr5.2:19300057-19300076 MS.gene027995:CDS 25.0%
! GAATGATTGTACTTACAATA+AGG + chr5.2:19300536-19300555 None:intergenic 25.0%
! GAGTAAATAATGAATGAGAT+TGG - chr5.2:19299752-19299771 MS.gene027995:intron 25.0%
! GCCATTGATAAAAATAAAAG+AGG - chr5.2:19300814-19300833 MS.gene027995:CDS 25.0%
! GTACACATTAATCTATTCTT+TGG - chr5.2:19298206-19298225 MS.gene027995:CDS 25.0%
! TATCAACACACATATATAAC+AGG + chr5.2:19300321-19300340 None:intergenic 25.0%
! TCGATTCAATATTGTATCTT+AGG - chr5.2:19299009-19299028 MS.gene027995:CDS 25.0%
! TGCAAACTTATAATGTTACA+CGG - chr5.2:19299277-19299296 MS.gene027995:CDS 25.0%
! TTTATAGATGCAATGAGAAA+GGG - chr5.2:19300772-19300791 MS.gene027995:CDS 25.0%
!! CTTATTGTGAAATTTTGTGT+AGG - chr5.2:19301507-19301526 MS.gene027995:intron 25.0%
!! GAGTATCATTCCAATTTTAT+CGG - chr5.2:19299706-19299725 MS.gene027995:intron 25.0%
!! TATATTTTCCTTATTCCTGA+TGG + chr5.2:19300610-19300629 None:intergenic 25.0%
!! TTGTATTTTATAAAGGACAC+TGG + chr5.2:19299437-19299456 None:intergenic 25.0%
!!! ATTTCGCTTACTTTTCAAAT+TGG - chr5.2:19299226-19299245 MS.gene027995:CDS 25.0%
!!! CTTTTACGCATTTTTTTTTC+AGG - chr5.2:19300351-19300370 MS.gene027995:intron 25.0%
!!! GCCTCTTTTATTTTTATCAA+TGG + chr5.2:19300818-19300837 None:intergenic 25.0%
!!! TTTCGCTTACTTTTCAAATT+GGG - chr5.2:19299227-19299246 MS.gene027995:CDS 25.0%
AAAGAAGCCTGGAATAAATT+GGG - chr5.2:19298112-19298131 MS.gene027995:CDS 30.0%
AAGAGAAATTCTATGGTTCA+AGG - chr5.2:19301029-19301048 MS.gene027995:CDS 30.0%
AATATATTCCACATCCTCTA+TGG - chr5.2:19298550-19298569 MS.gene027995:intron 30.0%
AATGAATACTACATGTGTAG+TGG + chr5.2:19299490-19299509 None:intergenic 30.0%
AATTGTATCCATCAGGAATA+AGG - chr5.2:19300599-19300618 MS.gene027995:intron 30.0%
AATTGTGAAGAAATTGGTGA+AGG - chr5.2:19298616-19298635 MS.gene027995:intron 30.0%
ACCATTGCGATAATTATCAT+CGG + chr5.2:19298891-19298910 None:intergenic 30.0%
AGACCAACTTTACAAAATTG+AGG - chr5.2:19299684-19299703 MS.gene027995:intron 30.0%
AGATGCAAAGAGAAATTCTA+TGG - chr5.2:19301022-19301041 MS.gene027995:CDS 30.0%
ATACATGATGCAACTGTAAA+TGG - chr5.2:19300886-19300905 MS.gene027995:CDS 30.0%
ATATATTCCACATCCTCTAT+GGG - chr5.2:19298551-19298570 MS.gene027995:intron 30.0%
ATATTAGGGTCCAATTAGTA+AGG - chr5.2:19298362-19298381 MS.gene027995:CDS 30.0%
ATCATGTATGAGTTCGAAAA+CGG + chr5.2:19300875-19300894 None:intergenic 30.0%
ATGCAAACTCGTTTATTAAG+AGG - chr5.2:19298516-19298535 MS.gene027995:intron 30.0%
ATTGTGAGTCTTGCAAATAT+TGG - chr5.2:19299912-19299931 MS.gene027995:intron 30.0%
CATATCATTCAACTAACATG+TGG - chr5.2:19298049-19298068 MS.gene027995:CDS 30.0%
CCAAAGAATTCAAGAGTTTA+AGG - chr5.2:19300681-19300700 MS.gene027995:intron 30.0%
CCTTAAACTCTTGAATTCTT+TGG + chr5.2:19300684-19300703 None:intergenic 30.0%
CTAAGACTGAATATGAGATT+TGG - chr5.2:19298002-19298021 MS.gene027995:CDS 30.0%
CTAATATGAGTAAGATTCTC+TGG + chr5.2:19298076-19298095 None:intergenic 30.0%
CTTGTTAGAAACTATCTTGT+GGG - chr5.2:19297929-19297948 MS.gene027995:CDS 30.0%
GGAGATGTTAAATTAAGTGT+TGG + chr5.2:19299469-19299488 None:intergenic 30.0%
GGTAATCTCAAAACTAAAAG+CGG + chr5.2:19299861-19299880 None:intergenic 30.0%
GTTTATAGATGCAATGAGAA+AGG - chr5.2:19300771-19300790 MS.gene027995:CDS 30.0%
TAAAGAAGCCTGGAATAAAT+TGG - chr5.2:19298111-19298130 MS.gene027995:CDS 30.0%
TACATGATCTGTTTGGAAAT+TGG - chr5.2:19299568-19299587 MS.gene027995:intron 30.0%
TATATACCTTGCTCAATATC+TGG + chr5.2:19298169-19298188 None:intergenic 30.0%
TCAATATTGTATCTTAGGTG+TGG - chr5.2:19299014-19299033 MS.gene027995:CDS 30.0%
TCAATATTTCAAGAGCTACA+CGG + chr5.2:19300651-19300670 None:intergenic 30.0%
TCTTGTTAGAAACTATCTTG+TGG - chr5.2:19297928-19297947 MS.gene027995:CDS 30.0%
TGCAAACTCGTTTATTAAGA+GGG - chr5.2:19298517-19298536 MS.gene027995:intron 30.0%
TGTATCACTGCAGAAATATT+TGG - chr5.2:19298929-19298948 MS.gene027995:CDS 30.0%
TTATCTATGAAGACCAATGA+TGG - chr5.2:19298779-19298798 MS.gene027995:intron 30.0%
TTTCACAATAAGTTATAGCG+CGG + chr5.2:19301499-19301518 None:intergenic 30.0%
! ACTCCTCAATTTTGTAAAGT+TGG + chr5.2:19299690-19299709 None:intergenic 30.0%
! AGAGTTTTACATGATCTGTT+TGG - chr5.2:19299561-19299580 MS.gene027995:intron 30.0%
! AGTGAAGTTTGATGAGTTTT+TGG + chr5.2:19299641-19299660 None:intergenic 30.0%
! GTTTTTCTACAATATGCGAA+TGG - chr5.2:19300029-19300048 MS.gene027995:CDS 30.0%
! TCACATGTCATTTCAGAATT+CGG - chr5.2:19300392-19300411 MS.gene027995:intron 30.0%
! TCTTTTCACTTCTTCAACTT+CGG - chr5.2:19301810-19301829 MS.gene027995:intron 30.0%
! TTATTCCTGATGGATACAAT+TGG + chr5.2:19300600-19300619 None:intergenic 30.0%
! TTTAAGTGTCTTTTGTGAGT+GGG - chr5.2:19298298-19298317 MS.gene027995:CDS 30.0%
!! AAGCTCTAGAAAGAAGAATT+AGG + chr5.2:19299990-19300009 None:intergenic 30.0%
!! ACATACATGCTTTAATTTGC+AGG - chr5.2:19298425-19298444 MS.gene027995:intron 30.0%
!! AGAGAAGTGCATGAATTTTA+CGG - chr5.2:19299885-19299904 MS.gene027995:intron 30.0%
!! TTTTAAGTGTCTTTTGTGAG+TGG - chr5.2:19298297-19298316 MS.gene027995:CDS 30.0%
!!! ATTTTTTGCTTCCTTACACT+TGG + chr5.2:19301554-19301573 None:intergenic 30.0%
!!! TTTTTTGCTTCCTTACACTT+GGG + chr5.2:19301553-19301572 None:intergenic 30.0%
AAAGATCACAGATCACAACA+AGG + chr5.2:19300223-19300242 None:intergenic 35.0%
AAGATCACAGATCACAACAA+GGG + chr5.2:19300222-19300241 None:intergenic 35.0%
AATTCATGCACTTCTCTTTC+AGG + chr5.2:19299882-19299901 None:intergenic 35.0%
ACTTGAAAATCTCAAGGAAG+AGG + chr5.2:19302068-19302087 None:intergenic 35.0%
AGAAACCACAGAGAAGAAAA+GGG + chr5.2:19301926-19301945 None:intergenic 35.0%
ATGATGCAACTGTAAATGGA+CGG - chr5.2:19300890-19300909 MS.gene027995:CDS 35.0%
ATTAATTAATCACAGAGGGG+AGG + chr5.2:19300558-19300577 None:intergenic 35.0%
CAATTCAAAGCTTCTTTCGT+AGG - chr5.2:19299948-19299967 MS.gene027995:intron 35.0%
CCACAAATCACACTTTGAAT+CGG - chr5.2:19299077-19299096 MS.gene027995:CDS 35.0%
CGAGGAAATTGTGAAGAAAT+TGG - chr5.2:19298610-19298629 MS.gene027995:intron 35.0%
CTGTAGACTTGAAAATCTCA+AGG + chr5.2:19302074-19302093 None:intergenic 35.0%
CTTTCTGTAAATAACTCACG+AGG + chr5.2:19301592-19301611 None:intergenic 35.0%
GAGAAGAGATTAGAGAGTTT+AGG - chr5.2:19301169-19301188 MS.gene027995:CDS 35.0%
GCTTATATGTTGGAACAAAG+AGG - chr5.2:19301108-19301127 MS.gene027995:CDS 35.0%
GTGAAAATGAAAGACAATCG+AGG - chr5.2:19298656-19298675 MS.gene027995:intron 35.0%
GTTATTTACAGAAAGCCACA+AGG - chr5.2:19301597-19301616 MS.gene027995:intron 35.0%
GTTGGTTGTGAAGTTGTTAT+AGG + chr5.2:19301295-19301314 None:intergenic 35.0%
TAAGAAAATATCACGGTCAG+AGG - chr5.2:19298751-19298770 MS.gene027995:intron 35.0%
TAAGGAATCAGAACTTAGAG+AGG - chr5.2:19300699-19300718 MS.gene027995:intron 35.0%
TAGAAACCACAGAGAAGAAA+AGG + chr5.2:19301927-19301946 None:intergenic 35.0%
TATAGCGAAGTGTATGACTA+TGG - chr5.2:19298724-19298743 MS.gene027995:intron 35.0%
TATAGCGCGGTTAAAATTCA+TGG + chr5.2:19301486-19301505 None:intergenic 35.0%
TATATTCCACATCCTCTATG+GGG - chr5.2:19298552-19298571 MS.gene027995:intron 35.0%
TCGGTACTATTAAAGTTCTC+TGG - chr5.2:19300411-19300430 MS.gene027995:intron 35.0%
TCTTGCAAATATTGGCAAAG+AGG - chr5.2:19299920-19299939 MS.gene027995:intron 35.0%
TGAAGAAATTGGTGAAGGAA+GGG - chr5.2:19298621-19298640 MS.gene027995:intron 35.0%
TGTGCAGCGATTTATATTGA+AGG + chr5.2:19298469-19298488 None:intergenic 35.0%
TTCATATTGCTAAAGAAGCC+TGG - chr5.2:19298101-19298120 MS.gene027995:CDS 35.0%
! AGATTTGGAACAGGAAGAAT+GGG - chr5.2:19298017-19298036 MS.gene027995:CDS 35.0%
! CGATTCAAAGTGTGATTTGT+GGG + chr5.2:19299079-19299098 None:intergenic 35.0%
! CTGAATATGAGATTTGGAAC+AGG - chr5.2:19298008-19298027 MS.gene027995:CDS 35.0%
! CTTTTCAAATTGGGTCATCT+CGG - chr5.2:19299236-19299255 MS.gene027995:CDS 35.0%
! GTTTCTTTAGGAAGGTTATG+TGG - chr5.2:19300082-19300101 MS.gene027995:intron 35.0%
! TTTTATCAATGGCCCATAAG+AGG + chr5.2:19300807-19300826 None:intergenic 35.0%
! TTTTTGCTTCCTTACACTTG+GGG + chr5.2:19301552-19301571 None:intergenic 35.0%
!! ATCTTCTCGGTTGTTTCTTT+AGG - chr5.2:19300070-19300089 MS.gene027995:CDS 35.0%
!! CATTGGTCTTCATAGATAAC+AGG + chr5.2:19298778-19298797 None:intergenic 35.0%
!! GAGAAGTGCATGAATTTTAC+GGG - chr5.2:19299886-19299905 MS.gene027995:intron 35.0%
!! TCACTTGCTATGCTCTTAAA+GGG - chr5.2:19301967-19301986 MS.gene027995:CDS 35.0%
!! TTCACTTGCTATGCTCTTAA+AGG - chr5.2:19301966-19301985 MS.gene027995:CDS 35.0%
AAATGGGCAAAAGATACAGC+TGG - chr5.2:19301640-19301659 MS.gene027995:intron 40.0%
AACAGGGATTTCACCATCAT+TGG + chr5.2:19298795-19298814 None:intergenic 40.0%
AACTTACCCAACTGCTTTAC+TGG + chr5.2:19300127-19300146 None:intergenic 40.0%
AATTGCCAAATGCGTCTACA+CGG + chr5.2:19300933-19300952 None:intergenic 40.0%
ACCTTAGTGGCAATGACTTA+AGG + chr5.2:19301885-19301904 None:intergenic 40.0%
ACCTTGCTCAATATCTGGAT+CGG + chr5.2:19298164-19298183 None:intergenic 40.0%
AGAAACTATCTTGTGGGACA+TGG - chr5.2:19297935-19297954 MS.gene027995:CDS 40.0%
AGTGCGAAAAGTAACCTTAG+TGG + chr5.2:19301898-19301917 None:intergenic 40.0%
AGTTGCACCCAATTTATTCC+AGG + chr5.2:19298122-19298141 None:intergenic 40.0%
ATCAGAACTTAGAGAGGCTT+CGG - chr5.2:19300705-19300724 MS.gene027995:intron 40.0%
CAAAGCTTCTTTCGTAGGTA+TGG - chr5.2:19299953-19299972 MS.gene027995:intron 40.0%
CACACATATATAACAGGGAC+TGG + chr5.2:19300315-19300334 None:intergenic 40.0%
CATGCATGCAGCTTATATGT+TGG - chr5.2:19301098-19301117 MS.gene027995:CDS 40.0%
CCAAAGACAACTACGAAAAC+TGG - chr5.2:19297900-19297919 MS.gene027995:CDS 40.0%
CCGATTCAAAGTGTGATTTG+TGG + chr5.2:19299080-19299099 None:intergenic 40.0%
CTGCACAAGTATATTGAGAG+CGG - chr5.2:19298482-19298501 MS.gene027995:intron 40.0%
CTTCGCTATATTCGTGTTTC+CGG + chr5.2:19298714-19298733 None:intergenic 40.0%
GCCGATGATAATTATCGCAA+TGG - chr5.2:19298887-19298906 MS.gene027995:CDS 40.0%
GCTTTGTATTCACACCCTTA+GGG + chr5.2:19300293-19300312 None:intergenic 40.0%
GGTGGAAACAATCAAGACAA+TGG - chr5.2:19301727-19301746 MS.gene027995:intron 40.0%
GTGAAGAAATTGGTGAAGGA+AGG - chr5.2:19298620-19298639 MS.gene027995:intron 40.0%
GTGACCTCTATTCCTGAAAT+TGG - chr5.2:19297971-19297990 MS.gene027995:CDS 40.0%
TAGAACGATAACAGGAACAC+TGG + chr5.2:19302028-19302047 None:intergenic 40.0%
TATTTCAAGAGCTACACGGT+AGG + chr5.2:19300647-19300666 None:intergenic 40.0%
TCCTTAAGTCATTGCCACTA+AGG - chr5.2:19301881-19301900 MS.gene027995:CDS 40.0%
TCTGATCTTGATCGTAGATC+CGG - chr5.2:19300988-19301007 MS.gene027995:intron 40.0%
TCTGGATTACAAGACGTATC+CGG + chr5.2:19300270-19300289 None:intergenic 40.0%
TCTTGTAATCCAGACCCTAA+GGG - chr5.2:19300276-19300295 MS.gene027995:intron 40.0%
TGCACTTGGCAGGATATTTA+GGG - chr5.2:19300959-19300978 MS.gene027995:intron 40.0%
TGTCTTGATTGTTTCCACCA+GGG + chr5.2:19301726-19301745 None:intergenic 40.0%
TGTGCAACCATTATACGCAT+TGG - chr5.2:19299104-19299123 MS.gene027995:CDS 40.0%
TTACTCCTCAATTGTCCCTT+CGG + chr5.2:19299738-19299757 None:intergenic 40.0%
TTATCGCCAGTAAAGCAGTT+GGG - chr5.2:19300118-19300137 MS.gene027995:intron 40.0%
TTGCACTTGGCAGGATATTT+AGG - chr5.2:19300958-19300977 MS.gene027995:intron 40.0%
TTGTCTTGATTGTTTCCACC+AGG + chr5.2:19301727-19301746 None:intergenic 40.0%
TTTATCGCCAGTAAAGCAGT+TGG - chr5.2:19300117-19300136 MS.gene027995:intron 40.0%
! AAATTTTGTGTAGGCAGTGG+AGG - chr5.2:19301516-19301535 MS.gene027995:intron 40.0%
! AGCTGGTTCATTTACTCTTG+TGG - chr5.2:19301657-19301676 MS.gene027995:intron 40.0%
! CCAGTTTTCGTAGTTGTCTT+TGG + chr5.2:19297903-19297922 None:intergenic 40.0%
! CTGCAGAAATATTTGGTTCG+TGG - chr5.2:19298936-19298955 MS.gene027995:CDS 40.0%
! CTTTAGGAAGGTTATGTGGA+CGG - chr5.2:19300086-19300105 MS.gene027995:intron 40.0%
! GAGATTTGGAACAGGAAGAA+TGG - chr5.2:19298016-19298035 MS.gene027995:CDS 40.0%
! GTGAAATTTTGTGTAGGCAG+TGG - chr5.2:19301513-19301532 MS.gene027995:intron 40.0%
! GTTCGAAAACGGCTTTTCTA+CGG + chr5.2:19300864-19300883 None:intergenic 40.0%
! TCCTGATGGATACAATTGGA+GGG + chr5.2:19300596-19300615 None:intergenic 40.0%
! TTCCTGATGGATACAATTGG+AGG + chr5.2:19300597-19300616 None:intergenic 40.0%
!! AAGCGGAAGTAGATTAGTCT+TGG + chr5.2:19299844-19299863 None:intergenic 40.0%
!! CATGCTTTAATTTGCAGGTG+TGG - chr5.2:19298430-19298449 MS.gene027995:intron 40.0%
!! TCTCGGTTGTTTCTTTAGGA+AGG - chr5.2:19300074-19300093 MS.gene027995:intron 40.0%
!! TTCCAAGTGGTAGTGGATAT+GGG - chr5.2:19299145-19299164 MS.gene027995:CDS 40.0%
!! TTTCCAAGTGGTAGTGGATA+TGG - chr5.2:19299144-19299163 MS.gene027995:CDS 40.0%
AAATGCCGTGTAGACGCATT+TGG - chr5.2:19300925-19300944 MS.gene027995:intron 45.0%
AACCCATATCCACTACCACT+TGG + chr5.2:19299150-19299169 None:intergenic 45.0%
AACTACCGCAACGTGAAGAA+CGG + chr5.2:19298582-19298601 None:intergenic 45.0%
AATTCCATGTTGCACTTGGC+AGG - chr5.2:19300949-19300968 MS.gene027995:intron 45.0%
AATTGTGCACCCCAAGTGTA+AGG - chr5.2:19301540-19301559 MS.gene027995:intron 45.0%
ACAAGGAGCTAGTGAAAGCT+GGG - chr5.2:19301614-19301633 MS.gene027995:intron 45.0%
ACTTCGGTCCTTCTCTTCAT+TGG - chr5.2:19301826-19301845 MS.gene027995:CDS 45.0%
AGGGTAATCCTGACCTCTTA+TGG - chr5.2:19300791-19300810 MS.gene027995:CDS 45.0%
AGTATATTGAGAGCGGTGAG+TGG - chr5.2:19298489-19298508 MS.gene027995:intron 45.0%
AGTGAAAGCTGGGGAGAAAT+GGG - chr5.2:19301624-19301643 MS.gene027995:intron 45.0%
ATATCCTGCCAAGTGCAACA+TGG + chr5.2:19300956-19300975 None:intergenic 45.0%
ATGCCTTCTGTGTTTCCAAG+TGG - chr5.2:19299132-19299151 MS.gene027995:CDS 45.0%
ATGCGTGCCAATGCGTATAA+TGG + chr5.2:19299114-19299133 None:intergenic 45.0%
ATGTTGCAAGCAGCAAAGCA+TGG - chr5.2:19300739-19300758 MS.gene027995:intron 45.0%
CATAGCAAGTGAAGCACAGA+AGG + chr5.2:19301959-19301978 None:intergenic 45.0%
CATGTATGTATGCGAGTGAG+TGG + chr5.2:19301082-19301101 None:intergenic 45.0%
CGCACCAATTTCAGGAATAG+AGG + chr5.2:19297978-19297997 None:intergenic 45.0%
CTACCACTTGGAAACACAGA+AGG + chr5.2:19299138-19299157 None:intergenic 45.0%
CTACGATCAAGATCAGAGGA+AGG + chr5.2:19300985-19301004 None:intergenic 45.0%
GACGGAACTAGAACGATAAC+AGG + chr5.2:19302036-19302055 None:intergenic 45.0%
GGATCTACGATCAAGATCAG+AGG + chr5.2:19300989-19301008 None:intergenic 45.0%
GGGTAATCCTGACCTCTTAT+GGG - chr5.2:19300792-19300811 MS.gene027995:CDS 45.0%
GTCTTGTAATCCAGACCCTA+AGG - chr5.2:19300275-19300294 MS.gene027995:intron 45.0%
GTGATGTCGTGCAAGAACAA+GGG + chr5.2:19301757-19301776 None:intergenic 45.0%
GTTCTTCTTGCTGCTGCTAA+GGG - chr5.2:19298812-19298831 MS.gene027995:intron 45.0%
TACTCCTCAATTGTCCCTTC+GGG + chr5.2:19299737-19299756 None:intergenic 45.0%
TAGCAGCAGCAAGAAGAACA+GGG + chr5.2:19298811-19298830 None:intergenic 45.0%
TAGTGAAAGCTGGGGAGAAA+TGG - chr5.2:19301623-19301642 MS.gene027995:intron 45.0%
TCCCTCCAATTGTATCCATC+AGG - chr5.2:19300592-19300611 MS.gene027995:intron 45.0%
TCGGGGACATCCGATAAAAT+TGG + chr5.2:19299719-19299738 None:intergenic 45.0%
TCGTAGTTGTCTTTGGTCAG+TGG + chr5.2:19297896-19297915 None:intergenic 45.0%
TCTCTTTGCATCTGCATAGC+AGG + chr5.2:19301015-19301034 None:intergenic 45.0%
TCTGTGTTTCCAAGTGGTAG+TGG - chr5.2:19299138-19299157 MS.gene027995:CDS 45.0%
TCTTTACCGTTGTACACCGT+TGG - chr5.2:19298856-19298875 MS.gene027995:CDS 45.0%
TGCAACTGTAAATGGACGGA+AGG - chr5.2:19300894-19300913 MS.gene027995:CDS 45.0%
TGCGATAATTATCATCGGCC+AGG + chr5.2:19298886-19298905 None:intergenic 45.0%
TGGCAATTCCATGTTGCACT+TGG - chr5.2:19300945-19300964 MS.gene027995:intron 45.0%
TGTGATGTCGTGCAAGAACA+AGG + chr5.2:19301758-19301777 None:intergenic 45.0%
TGTTCTTCTTGCTGCTGCTA+AGG - chr5.2:19298811-19298830 MS.gene027995:intron 45.0%
TGTTGCAAGCAGCAAAGCAT+GGG - chr5.2:19300740-19300759 MS.gene027995:intron 45.0%
TTAGAACGCGCACCAATTTC+AGG + chr5.2:19297986-19298005 None:intergenic 45.0%
TTAGCAGCAGCAAGAAGAAC+AGG + chr5.2:19298812-19298831 None:intergenic 45.0%
TTGGAACAGGAAGAATGGGA+AGG - chr5.2:19298021-19298040 MS.gene027995:CDS 45.0%
! AAGTGTGATTTGTGGGCAAG+AGG + chr5.2:19299072-19299091 None:intergenic 45.0%
! ATTTTATCGGATGTCCCCGA+AGG - chr5.2:19299719-19299738 MS.gene027995:intron 45.0%
! CATTGCCCTTTTCTTCTCTG+TGG - chr5.2:19301918-19301937 MS.gene027995:CDS 45.0%
! GAGGCATTTGAGGAAATCGT+TGG + chr5.2:19299053-19299072 None:intergenic 45.0%
! GGCTTTGTATTCACACCCTT+AGG + chr5.2:19300294-19300313 None:intergenic 45.0%
! TTTTATCGGATGTCCCCGAA+GGG - chr5.2:19299720-19299739 MS.gene027995:intron 45.0%
!! GCTTTAATTTGCAGGTGTGG+TGG - chr5.2:19298433-19298452 MS.gene027995:intron 45.0%
ACTCCTCAATTGTCCCTTCG+GGG + chr5.2:19299736-19299755 None:intergenic 50.0%
ATCTTGTGGGACATGGTCTG+TGG - chr5.2:19297942-19297961 MS.gene027995:CDS 50.0%
ATTCCACATCCTCTATGGGG+AGG - chr5.2:19298555-19298574 MS.gene027995:intron 50.0%
CAAGGAGCTAGTGAAAGCTG+GGG - chr5.2:19301615-19301634 MS.gene027995:intron 50.0%
CACAAGGAGCTAGTGAAAGC+TGG - chr5.2:19301613-19301632 MS.gene027995:intron 50.0%
CAGCTCAGCAACAAGAGCTA+GGG + chr5.2:19298690-19298709 None:intergenic 50.0%
CAGCTTTCACTAGCTCCTTG+TGG + chr5.2:19301615-19301634 None:intergenic 50.0%
CTCACGAGGCTTCTTATCGT+CGG + chr5.2:19301578-19301597 None:intergenic 50.0%
GCCGATCCAGATATTGAGCA+AGG - chr5.2:19298160-19298179 MS.gene027995:CDS 50.0%
GGTCTTCCAACGGTGTACAA+CGG + chr5.2:19298865-19298884 None:intergenic 50.0%
GTATTCACACCCTTAGGGTC+TGG + chr5.2:19300288-19300307 None:intergenic 50.0%
TCAATGGCCCATAAGAGGTC+AGG + chr5.2:19300802-19300821 None:intergenic 50.0%
TCACAACAAGGGTTCGCACA+AGG + chr5.2:19300211-19300230 None:intergenic 50.0%
TCAGCTCAGCAACAAGAGCT+AGG + chr5.2:19298691-19298710 None:intergenic 50.0%
TCCGGTGCAAAGCACACAAA+CGG + chr5.2:19300252-19300271 None:intergenic 50.0%
TCCGTTTGTGTGCTTTGCAC+CGG - chr5.2:19300248-19300267 MS.gene027995:intron 50.0%
TCTTGTGGGACATGGTCTGT+GGG - chr5.2:19297943-19297962 MS.gene027995:CDS 50.0%
TTCACGTTGCGGTAGTTGCT+GGG - chr5.2:19298585-19298604 MS.gene027995:intron 50.0%
TTGCATCTGCATAGCAGGAC+CGG + chr5.2:19301010-19301029 None:intergenic 50.0%
TTGTACACCGTTGGAAGACC+TGG - chr5.2:19298865-19298884 MS.gene027995:CDS 50.0%
TTTCAGCGTAACGAGCCGTT+AGG + chr5.2:19301855-19301874 None:intergenic 50.0%
! AGGAAGAGGCGCAATTGTGA+CGG + chr5.2:19302054-19302073 None:intergenic 50.0%
!! TTGTGGGCAAGAGGCATTTG+AGG + chr5.2:19299063-19299082 None:intergenic 50.0%
AAGAACGGTCCTCCCCATAG+AGG + chr5.2:19298567-19298586 None:intergenic 55.0%
CTTCACGTTGCGGTAGTTGC+TGG - chr5.2:19298584-19298603 MS.gene027995:intron 55.0%
CTTGTGGGACATGGTCTGTG+GGG - chr5.2:19297944-19297963 MS.gene027995:CDS 55.0%
GAGGACCGTTCTTCACGTTG+CGG - chr5.2:19298574-19298593 MS.gene027995:intron 55.0%
GCTCTTGTTGCTGAGCTGAC+CGG - chr5.2:19298692-19298711 MS.gene027995:intron 55.0%
TCATCGGCCAGGTCTTCCAA+CGG + chr5.2:19298875-19298894 None:intergenic 55.0%
TGTCCCCGAAGGGACAATTG+AGG - chr5.2:19299730-19299749 MS.gene027995:intron 55.0%
! GGAACAAGCATTGCAGCGAG+TGG - chr5.2:19297857-19297876 MS.gene027995:CDS 55.0%
GCAGCTTTCACTGTCCCTGG+TGG - chr5.2:19301709-19301728 MS.gene027995:intron 60.0%
GCTGCAGCTTTCACTGTCCC+TGG - chr5.2:19301706-19301725 MS.gene027995:intron 60.0%
GGTCCTCCCCATAGAGGATG+TGG + chr5.2:19298561-19298580 None:intergenic 60.0%
TGCGGTAGTTGCTGGGCACG+AGG - chr5.2:19298592-19298611 MS.gene027995:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 19297851 19302107 19297851 ID=MS.gene027995
chr5.2 mRNA 19297851 19302107 19297851 ID=MS.gene027995.t1;Parent=MS.gene027995
chr5.2 exon 19301825 19302107 19301825 ID=MS.gene027995.t1.exon1;Parent=MS.gene027995.t1
chr5.2 CDS 19301825 19302107 19301825 ID=cds.MS.gene027995.t1;Parent=MS.gene027995.t1
chr5.2 exon 19301008 19301511 19301008 ID=MS.gene027995.t1.exon2;Parent=MS.gene027995.t1
chr5.2 CDS 19301008 19301511 19301008 ID=cds.MS.gene027995.t1;Parent=MS.gene027995.t1
chr5.2 exon 19300766 19300927 19300766 ID=MS.gene027995.t1.exon3;Parent=MS.gene027995.t1
chr5.2 CDS 19300766 19300927 19300766 ID=cds.MS.gene027995.t1;Parent=MS.gene027995.t1
chr5.2 exon 19299989 19300090 19299989 ID=MS.gene027995.t1.exon4;Parent=MS.gene027995.t1
chr5.2 CDS 19299989 19300090 19299989 ID=cds.MS.gene027995.t1;Parent=MS.gene027995.t1
chr5.2 exon 19299819 19299866 19299819 ID=MS.gene027995.t1.exon5;Parent=MS.gene027995.t1
chr5.2 CDS 19299819 19299866 19299819 ID=cds.MS.gene027995.t1;Parent=MS.gene027995.t1
chr5.2 exon 19298829 19299344 19298829 ID=MS.gene027995.t1.exon6;Parent=MS.gene027995.t1
chr5.2 CDS 19298829 19299344 19298829 ID=cds.MS.gene027995.t1;Parent=MS.gene027995.t1
chr5.2 exon 19297851 19298425 19297851 ID=MS.gene027995.t1.exon7;Parent=MS.gene027995.t1
chr5.2 CDS 19297851 19298425 19297851 ID=cds.MS.gene027995.t1;Parent=MS.gene027995.t1
Gene Sequence

>MS.gene027995

ATGACAGGAACAAGCATTGCAGCGAGTGGAATAGTTCTTGAACCACTGACCAAAGACAACTACGAAAACTGGAGCTGTCTTGTTAGAAACTATCTTGTGGGACATGGTCTGTGGGGTGTTGTGACCTCTATTCCTGAAATTGGTGCGCGTTCTAAGACTGAATATGAGATTTGGAACAGGAAGAATGGGAAGGCATTGCATATCATTCAACTAACATGTGGACCAGAGAATCTTACTCATATTAGAGATCTTCATATTGCTAAAGAAGCCTGGAATAAATTGGGTGCAACTTATAGCTCAGATTTACAAGCCGATCCAGATATTGAGCAAGGTATATATGTAAATTTATATATATGTACACATTAATCTATTCTTTGGGTAAATCACGTAATGATCCCTATACATATTAGACATAGTTTCTAAATGTATTGAAAGGACAAAAGTATTTTTAAGTGTCTTTTGTGAGTGGGACACTAAAATAGATCTTCACTCATTTTCTTAAATACTTATAATATTAGGGTCCAATTAGTAAGGAAAATAAATGGAAGAAATATGTATATGTTTGTAGCTTAAAACATACATGCTTTAATTTGCAGGTGTGGTGGATGATAGTCTCCTTCAATATAAATCGCTGCACAAGTATATTGAGAGCGGTGAGTGGAAAGATGCAAACTCGTTTATTAAGAGGGACAGTACAGCAATATATTCCACATCCTCTATGGGGAGGACCGTTCTTCACGTTGCGGTAGTTGCTGGGCACGAGGAAATTGTGAAGAAATTGGTGAAGGAAGGGAAAGATAAGTTAGTGAAAATGAAAGACAATCGAGGTTACACAGCCCTAGCTCTTGTTGCTGAGCTGACCGGAAACACGAATATAGCGAAGTGTATGACTATGGTATATAAGAAAATATCACGGTCAGAGGACCTGTTATCTATGAAGACCAATGATGGTGAAATCCCTGTTCTTCTTGCTGCTGCTAAGGGACACAAAGAAATGACACGTTATCTTTACCGTTGTACACCGTTGGAAGACCTGGCCGATGATAATTATCGCAATGGTGTTTTGCTTCTTACACGATGTATCACTGCAGAAATATTTGGTTCGTGGTTACTGAATTTTAATTAAATTGAGGGTATTTTTGATATTAGTGTTTGATGTCGATTCAATATTGTATCTTAGGTGTGGCTTTGAGTTTACTCCAACGATTTCCTCAAATGCCTCTTGCCCACAAATCACACTTTGAATCGGATTGTGTGCAACCATTATACGCATTGGCACGCATGCCTTCTGTGTTTCCAAGTGGTAGTGGATATGGGTTCATACGACAATTCTTTAACGATAGTAAGTTTTTTTTTTTGTTTTTTTTCTGTTTCCATTTCGCTTACTTTTCAAATTGGGTCATCTCGGTAGTGAAATAGCTAGTTATGCAAACTTATAATGTTACACGGTGAAATGAATTTTTATACAAATCTAAAATTATTTATACGTTGTTGAGATACATAAAGATAAATATTGTTTAATAAATGATCAATATAACTTTATAAAAGAAATTTAAAGGTGATTTATAAAAGGGAAATGCTAACCAGTGTCCTTTATAAAATACAAACTTTTGATCCAACACTTAATTTAACATCTCCACTACACATGTAGTATTCATTTTATCATCTTTACTTCCAATAATATATTAAAGGAGTTTTACTTTAATCAAAAGAGTTTTACATGATCTGTTTGGAAATTGGAAGCATTATATACTATGCTGAATATTTTAAAACTCGATCTTGTGTATCCAAAAACTCATCAAACTTCACTCTTGTGCGATACTTTTCTTCTCAAGACCAACTTTACAAAATTGAGGAGTATCATTCCAATTTTATCGGATGTCCCCGAAGGGACAATTGAGGAGTAAATAATGAATGAGATTGGAATAAAAATCATCAAACAAGATAAATACTCAAATTTTGCATTCAAGTGTTTTCTTTCTGTCTGCATCCAAGACTAATCTACTTCCGCTTTTAGTTTTGAGATTACCTGAAAGAGAAGTGCATGAATTTTACGGGATAATTGTGAGTCTTGCAAATATTGGCAAAGAGGAAAAACAATTCAAAGCTTCTTTCGTAGGTATGGAATATTACACTCCTAATTCTTCTTTCTAGAGCTTATTATGTTCATGCAAGCAAGTTTTTCTACAATATGCGAATGGTGATTCTGAAGATTATTAATCTTCTCGGTTGTTTCTTTAGGAAGGTTATGTGGACGGATGTTGAATTTATCGCCAGTAAAGCAGTTGGGTAAGTTTTTTATCTATCAAATTATTTTATATATCAATTTTTTTTTTAATAATTTTTATATATCAAAGCCTTGTGCGAACCCTTGTTGTGATCTGTGATCTTTTTGTTTCCGTTTGTGTGCTTTGCACCGGATACGTCTTGTAATCCAGACCCTAAGGGTGTGAATACAAAGCCAGTCCCTGTTATATATGTGTGTTGATATGTCATTTTTCTTTTACGCATTTTTTTTTCAGGTCGACTACTCATACTTGTTCACATGTCATTTCAGAATTCGGTACTATTAAAGTTCTCTGGTATGTAATTATTAATCCTTGTTTAAATAGGTATATTAGATTGATAATTAATTATTAATCCTTGTTTAAATAGGTATATTAGATTGATAATTAAGATTATCCTTATTGTAAGTACAATCATTCCTCCCCTCTGTGATTAATTAATTAAATTTATTTAAATCCCTCCAATTGTATCCATCAGGAATAAGGAAAATATATAATCAGAAAATGACCTACCGTGTAGCTCTTGAAATATTGAGTTGCTTGCACCAAAGAATTCAAGAGTTTAAGGAATCAGAACTTAGAGAGGCTTCGGCTTACGATGCAATGTTGCAAGCAGCAAAGCATGGGATTATTGAGTTTATAGATGCAATGAGAAAGGGTAATCCTGACCTCTTATGGGCCATTGATAAAAATAAAAGAGGCGTATTTTCACATGCAATTTTGAACCGTAGAAAAGCCGTTTTCGAACTCATACATGATGCAACTGTAAATGGACGGAAGGAGATAGTTAAATGCCGTGTAGACGCATTTGGCAATTCCATGTTGCACTTGGCAGGATATTTAGGGCCTTCCTCTGATCTTGATCGTAGATCCGGTCCTGCTATGCAGATGCAAAGAGAAATTCTATGGTTCAAGGTGATTTCTTATGTCTCGTCTTTTTTTACCACTCACTCGCATACATACATGCATGCAGCTTATATGTTGGAACAAAGAGGTTATTTGATGATTTTGAGAGATAATAGAAGAGAGAACTGAGAAGAGATTAGAGAGTTTAGGAGAAAATAGACTTGTATTGTATGTTGAAAAATTCTCTAATACAAACACATATGTGAGCTTGTCTATTTATAGACTACATGACTTGAGATACAAGTTAGCTCCTATAACAACTTCACAACCAACTTCAAAAACTAAATTTGAAATTCTGGAAACATTTATAATTTATTATAGTCAACATCCAATTTTGAGATACATGACTTTTAAATAAGTGGTTAATTACGTTCTACAAATGATTAATGATTAGCCACACATTATTATCTTATTCATTAAGTGTTCACTGAATGTAATTAACCATGAATTTTAACCGCGCTATAACTTATTGTGAAATTTTGTGTAGGCAGTGGAGGAAATTGTGCACCCCAAGTGTAAGGAAGCAAAAAATGCCGACGATAAGAAGCCTCGTGAGTTATTTACAGAAAGCCACAAGGAGCTAGTGAAAGCTGGGGAGAAATGGGCAAAAGATACAGCTGGTTCATTTACTCTTGTGGCTACACTCATCACTACTATCATGTTTGCTGCAGCTTTCACTGTCCCTGGTGGAAACAATCAAGACAATGGAGTTCCCTTGTTCTTGCACGACATCACATTTGATGTGTTTATTATAGCAGATGCAGCATCTCTTTTCACTTCTTCAACTTCGGTCCTTCTCTTCATTGGAATCCTAACGGCTCGTTACGCTGAAAAAGATTTCCTTAAGTCATTGCCACTAAGGTTACTTTTCGCACTCATTGCCCTTTTCTTCTCTGTGGTTTCTATGATAGTTGCCTTCTGTGCTTCACTTGCTATGCTCTTAAAGGGACATCATAGAGTCATAATAACAGCAATGTCATTTGCCAGTGTTCCTGTTATCGTTCTAGTTCCGTCACAATTGCGCCTCTTCCTTGAGATTTTCAAGTCTACAGTATTATCCAATTGA
Protein sequence

>MS.gene027995.t1

MTGTSIAASGIVLEPLTKDNYENWSCLVRNYLVGHGLWGVVTSIPEIGARSKTEYEIWNRKNGKALHIIQLTCGPENLTHIRDLHIAKEAWNKLGVVDDSLLQYKSLHKYIESGEWKDANSFIKRDSTAIYSTSSMGRTVLHVAVVAGHEEIVKKLVKEGKDKLVKMKDNRGYTALALVAELTGNTNIAKCMTMVYKKISRSEDLLSMKTNDGEIPVLLAAAKGHKEMTRYLYRCTPLEDLADDNYRNGVLLLTRCITAEIFGVALSLLQRFPQMPLAHKSHFESDCVQPLYALARMPSVFPSGSGYGFIRQFFNDILRLPEREVHEFYGIIVSLANIGKEEKQFKASFVGRLCGRMLNLSPVKQLGIRKIYNQKMTYRVALEILSCLHQRIQEFKESELREASAYDAMLQAAKHGIIEFIDAMRKGNPDLLWAIDKNKRGVFSHAILNRRKAVFELIHDATVNGRKEIVKCRVDAFGNSMLHLAGYLGPSSDLDRRSGPAMQMQREILWFKVISYVSSFFTTHSHTYMHAAYMLEQRVEEIVHPKCKEAKNADDKKPRELFTESHKELVKAGEKWAKDTAGSFTLVATLITTIMFAAAFTVPGGNNQDNGVPLFLHDITFDVFIIADAASLFTSSTSVLLFIGILTARYAEKDFLKSLPLRLLFALIALFFSVVSMIVAFCASLAMLLKGHHRVIITAMSFASVPVIVLVPSQLRLFLEIFKSTVLSN