AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028530.t1 MTR_4g092700 95.161 310 15 0 1 310 24 333 0.0 613
MS.gene028530.t1 MTR_2g010450 53.295 349 119 9 4 310 7 353 1.51e-110 325
MS.gene028530.t1 MTR_5g014600 54.011 187 71 3 134 306 148 333 8.43e-57 186
MS.gene028530.t1 MTR_4g097940 37.910 335 176 9 1 310 1 328 7.36e-55 181
MS.gene028530.t1 MTR_4g097920 37.313 335 178 9 1 310 1 328 1.48e-52 175
MS.gene028530.t1 MTR_4g097920 42.529 261 139 6 54 310 60 313 1.69e-51 172
MS.gene028530.t1 MTR_5g014520 49.198 187 93 2 118 303 140 325 3.51e-51 172
MS.gene028530.t1 MTR_4g097950 54.749 179 68 4 135 303 70 245 2.71e-45 154
MS.gene028530.t1 MTR_0250s0040 44.809 183 89 3 134 310 87 263 6.06e-44 151
MS.gene028530.t1 MTR_5g014640 39.409 203 110 3 113 306 124 322 6.65e-44 153
MS.gene028530.t1 MTR_8g009020 40.179 224 112 4 100 303 97 318 1.97e-43 152
MS.gene028530.t1 MTR_2g104550 46.857 175 88 2 135 306 138 310 2.96e-42 148
MS.gene028530.t1 MTR_6g047570 43.979 191 104 3 115 303 138 327 4.20e-42 148
MS.gene028530.t1 MTR_0246s0020 44.724 199 100 5 119 310 182 377 4.26e-42 149
MS.gene028530.t1 MTR_5g014560 43.931 173 95 2 134 305 130 301 7.77e-41 144
MS.gene028530.t1 MTR_0011s0210 41.808 177 103 0 134 310 182 358 8.57e-40 143
MS.gene028530.t1 MTR_4g066460 40.000 215 107 5 107 309 122 326 1.98e-39 141
MS.gene028530.t1 MTR_0011s0260 42.373 177 102 0 134 310 182 358 2.07e-39 142
MS.gene028530.t1 MTR_4g009540 43.820 178 94 2 135 306 152 329 5.62e-39 140
MS.gene028530.t1 MTR_4g009110 43.429 175 96 1 135 306 174 348 2.78e-37 136
MS.gene028530.t1 MTR_8g009010 41.011 178 68 2 134 310 133 274 2.98e-37 135
MS.gene028530.t1 MTR_0246s0020 46.591 176 84 5 119 287 182 354 6.37e-37 135
MS.gene028530.t1 MTR_4g067010 39.583 192 100 4 125 310 138 319 1.11e-36 134
MS.gene028530.t1 MTR_4g066380 37.915 211 118 4 107 310 120 324 1.57e-36 134
MS.gene028530.t1 MTR_2g104490 43.103 174 97 1 135 308 137 308 3.87e-36 132
MS.gene028530.t1 MTR_7g080780 43.158 190 96 5 115 303 133 311 6.57e-36 132
MS.gene028530.t1 MTR_7g080780 43.158 190 96 5 115 303 146 324 7.21e-36 132
MS.gene028530.t1 MTR_4g098035 40.449 178 97 3 134 310 171 340 6.67e-35 129
MS.gene028530.t1 MTR_0246s0050 40.449 178 97 3 134 310 150 319 7.05e-35 129
MS.gene028530.t1 MTR_2g104560 38.636 176 94 4 134 303 53 220 3.46e-34 125
MS.gene028530.t1 MTR_4g009540 44.025 159 83 2 135 287 152 310 1.91e-33 125
MS.gene028530.t1 MTR_2g104490 41.615 161 92 1 135 295 137 295 2.99e-32 121
MS.gene028530.t1 MTR_2g104530 35.838 173 107 2 136 308 106 274 1.05e-30 117
MS.gene028530.t1 MTR_2g104590 38.418 177 83 4 135 307 69 223 1.29e-30 115
MS.gene028530.t1 MTR_8g069740 38.798 183 98 5 110 286 91 265 2.51e-29 113
MS.gene028530.t1 MTR_2g104650 38.119 202 115 5 110 307 73 268 2.00e-28 110
MS.gene028530.t1 MTR_2g104500 34.911 169 105 1 135 303 104 267 1.07e-27 108
MS.gene028530.t1 MTR_4g009510 36.723 177 98 5 133 308 42 205 6.20e-24 97.1
MS.gene028530.t1 MTR_7g451650 35.948 153 88 2 156 308 55 197 4.81e-23 94.7
MS.gene028530.t1 MTR_5g014550 41.732 127 72 2 165 290 54 179 1.02e-22 93.6
MS.gene028530.t1 MTR_4g009420 35.294 153 81 2 156 308 63 197 8.61e-22 91.3
MS.gene028530.t1 MTR_6g047550 64.286 70 24 1 134 203 172 240 4.90e-21 90.9
MS.gene028530.t1 MTR_7g050350 35.374 147 81 2 164 308 1 135 1.29e-20 86.3
MS.gene028530.t1 MTR_7g451650 35.374 147 81 2 164 308 1 135 1.29e-20 86.3
MS.gene028530.t1 MTR_7g451650 36.029 136 77 2 156 291 55 180 2.14e-19 84.7
MS.gene028530.t1 MTR_4g009420 35.294 136 70 2 156 291 63 180 5.56e-18 80.9
MS.gene028530.t1 MTR_7g096350 53.125 64 30 0 133 196 353 416 4.49e-16 79.0
MS.gene028530.t1 MTR_8g027495 38.053 113 62 3 133 245 456 560 5.21e-16 79.0
MS.gene028530.t1 MTR_1g072320 29.310 174 113 3 103 266 436 609 8.33e-16 78.2
MS.gene028530.t1 MTR_7g117670 34.058 138 81 3 133 269 400 528 1.89e-15 77.0
MS.gene028530.t1 MTR_8g067280 30.380 158 81 3 133 270 460 608 5.97e-15 75.9
MS.gene028530.t1 MTR_8g024790 29.091 165 98 4 120 273 274 430 1.75e-14 73.9
MS.gene028530.t1 MTR_3g498695 27.711 166 104 3 129 281 292 454 2.67e-14 73.6
MS.gene028530.t1 MTR_3g498695 27.711 166 104 3 129 281 292 454 2.78e-14 73.6
MS.gene028530.t1 MTR_3g030700 26.804 194 115 4 106 286 173 352 7.46e-14 71.6
MS.gene028530.t1 MTR_8g098275 40.476 84 45 1 113 191 217 300 4.23e-13 69.7
MS.gene028530.t1 MTR_8g098275 46.269 67 36 0 125 191 298 364 5.45e-13 69.7
MS.gene028530.t1 MTR_5g030430 28.188 149 96 2 133 270 491 639 6.35e-13 69.7
MS.gene028530.t1 MTR_7g083900 29.012 162 103 4 132 281 283 444 7.11e-13 69.3
MS.gene028530.t1 MTR_7g083900 29.012 162 103 4 132 281 283 444 7.16e-13 69.3
MS.gene028530.t1 MTR_8g098275 46.269 67 36 0 125 191 391 457 7.56e-13 69.3
MS.gene028530.t1 MTR_8g064700 30.323 155 99 3 137 284 246 398 4.49e-12 66.6
MS.gene028530.t1 MTR_3g104100 25.882 170 94 4 133 281 193 351 5.96e-12 66.2
MS.gene028530.t1 MTR_8g024770 26.630 184 117 4 120 291 262 439 9.68e-12 65.9
MS.gene028530.t1 MTR_2g038040 26.829 164 103 4 136 284 53 214 1.08e-11 64.3
MS.gene028530.t1 MTR_2g084280 30.216 139 91 3 137 273 135 269 1.20e-11 64.7
MS.gene028530.t1 MTR_1g112680 49.091 55 28 0 136 190 318 372 2.58e-11 64.7
MS.gene028530.t1 MTR_2g081040 25.882 170 94 4 133 281 175 333 4.17e-11 63.5
MS.gene028530.t1 MTR_5g030770 22.297 296 187 8 37 302 32 314 5.20e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028530.t1 AT4G37850 55.556 180 75 3 134 310 151 328 1.11e-54 181
MS.gene028530.t1 AT4G37850 55.556 180 75 3 134 310 151 328 1.11e-54 181
MS.gene028530.t1 AT2G22760 47.727 176 88 2 132 303 116 291 1.41e-48 164
MS.gene028530.t1 AT2G22750 49.309 217 96 7 93 302 88 297 1.31e-44 154
MS.gene028530.t1 AT2G22750 49.309 217 96 7 93 302 116 325 1.63e-44 155
MS.gene028530.t1 AT2G22750 49.309 217 96 7 93 302 112 321 2.12e-44 154
MS.gene028530.t1 AT2G22750 49.309 217 95 8 93 302 88 296 4.00e-43 150
MS.gene028530.t1 AT2G22750 49.309 217 95 8 93 302 116 324 5.28e-43 150
MS.gene028530.t1 AT2G22770 43.850 187 90 2 132 303 129 315 1.56e-40 144
MS.gene028530.t1 AT2G22760 45.283 159 84 1 132 287 116 274 1.77e-40 143
MS.gene028530.t1 AT2G46510 42.342 111 46 2 133 243 393 485 4.89e-18 84.7
MS.gene028530.t1 AT1G32640 34.965 143 86 2 133 271 450 589 1.14e-15 77.8
MS.gene028530.t1 AT1G01260 36.036 111 67 2 83 191 381 489 1.97e-14 73.9
MS.gene028530.t1 AT1G01260 36.036 111 67 2 83 191 381 489 1.97e-14 73.9
MS.gene028530.t1 AT1G01260 36.036 111 67 2 83 191 381 489 1.97e-14 73.9
MS.gene028530.t1 AT5G46760 31.579 152 82 4 133 271 413 555 3.40e-14 73.6
MS.gene028530.t1 AT4G17880 30.556 144 90 3 133 271 414 552 5.63e-14 72.8
MS.gene028530.t1 AT4G29930 28.492 179 95 5 124 290 46 203 7.51e-14 70.1
MS.gene028530.t1 AT2G16910 29.897 194 100 7 136 301 315 500 9.61e-14 72.0
MS.gene028530.t1 AT4G29930 30.952 168 102 6 124 290 46 200 1.27e-13 69.3
MS.gene028530.t1 AT1G12860 29.299 157 103 4 132 281 264 419 1.54e-13 71.2
MS.gene028530.t1 AT4G16430 48.438 64 33 0 135 198 320 383 1.62e-13 71.2
MS.gene028530.t1 AT1G12860 29.299 157 103 4 132 281 264 419 1.84e-13 70.9
MS.gene028530.t1 AT3G26744 29.193 161 102 4 132 281 304 463 2.13e-13 70.9
MS.gene028530.t1 AT5G57150 32.903 155 83 4 136 275 56 204 2.38e-13 68.6
MS.gene028530.t1 AT3G26744 29.193 161 102 4 132 281 304 463 2.47e-13 70.5
MS.gene028530.t1 AT3G26744 29.193 161 102 4 132 281 304 463 2.47e-13 70.5
MS.gene028530.t1 AT3G26744 29.193 161 102 4 132 281 304 463 2.47e-13 70.5
MS.gene028530.t1 AT5G57150 32.903 155 83 4 136 275 55 203 2.49e-13 68.6
MS.gene028530.t1 AT5G57150 32.903 155 83 4 136 275 56 204 2.81e-13 68.6
MS.gene028530.t1 AT5G57150 32.903 155 83 4 136 275 55 203 3.59e-13 68.6
MS.gene028530.t1 AT4G09820 30.282 142 70 4 132 267 357 475 7.41e-13 69.3
MS.gene028530.t1 AT5G57150 32.903 155 83 4 136 275 56 204 7.46e-13 67.8
MS.gene028530.t1 AT4G29930 29.697 165 102 5 124 287 46 197 9.06e-13 67.0
MS.gene028530.t1 AT1G63650 38.554 83 51 0 135 217 405 487 9.55e-13 68.9
MS.gene028530.t1 AT1G63650 38.554 83 51 0 135 217 405 487 9.55e-13 68.9
MS.gene028530.t1 AT1G63650 38.554 83 51 0 135 217 405 487 9.55e-13 68.9
MS.gene028530.t1 AT4G29930 28.723 188 109 7 124 290 46 229 1.68e-11 63.9
MS.gene028530.t1 AT3G61950 30.556 144 92 4 135 273 128 268 1.82e-11 64.3
MS.gene028530.t1 AT3G61950 30.556 144 92 4 135 273 128 268 2.94e-11 63.5
MS.gene028530.t1 AT5G41315 35.789 95 53 2 102 188 405 499 4.24e-11 63.9
MS.gene028530.t1 AT3G61950 31.387 137 86 4 135 266 179 312 4.30e-11 63.5
MS.gene028530.t1 AT5G41315 35.789 95 53 2 102 188 408 502 4.53e-11 63.9
MS.gene028530.t1 AT5G41315 35.789 95 53 2 102 188 400 494 4.54e-11 63.9
MS.gene028530.t1 AT4G29930 44.776 67 34 1 124 190 46 109 4.83e-11 61.2
MS.gene028530.t1 AT3G61950 30.556 144 92 4 135 273 179 319 6.48e-11 62.8

Find 57 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATCTTGCTTCAATTTC+TGG 0.046048 4.1:-20515619 None:intergenic
GAAAATTCTTCTGCAGAATT+TGG 0.250591 4.1:+20515574 MS.gene028530:CDS
GCAGTTTCCATTGAAGTTCT+AGG 0.252953 4.1:-20514838 None:intergenic
ATGGACAAAGTTACTGTTCT+TGG 0.276006 4.1:+20515427 MS.gene028530:CDS
AATAGCAGTTTCATGACATT+TGG 0.296146 4.1:+20515739 MS.gene028530:CDS
GCTTTCTTTCAGATATTATA+TGG 0.308934 4.1:-20515068 None:intergenic
CTGCCAATAGGTACTGCTTT+TGG 0.314519 4.1:+20514772 MS.gene028530:CDS
GCTCTATCTTCCCTTGTTCC+CGG 0.327620 4.1:+20515120 MS.gene028530:CDS
GTGCAATTTGCAGATGGATA+TGG 0.328392 4.1:+20515970 MS.gene028530:intron
GATTGAGTTTCTCAATTTCA+TGG 0.328907 4.1:-20515705 None:intergenic
GAACCAATCATCTCATCCTT+AGG 0.367456 4.1:-20514949 None:intergenic
AGGAACCTTTGAGGCCAAAA+AGG 0.379217 4.1:+20515011 MS.gene028530:CDS
TCGACAAATGAAAGAAGATT+CGG 0.392423 4.1:-20514892 None:intergenic
GAAGTTCTAGGGTTGAAGTT+TGG 0.405401 4.1:-20514826 None:intergenic
TATCAGAGGGTTTCCTGAGC+AGG 0.415708 4.1:+20514473 MS.gene028530:CDS
ATAGCAGTTTCATGACATTT+GGG 0.420095 4.1:+20515740 MS.gene028530:CDS
TTTGAATCAGTTAAATCAGT+TGG 0.433019 4.1:+20514915 MS.gene028530:CDS
CCTTGTTCCCGGACTACAAA+AGG 0.436297 4.1:+20515131 MS.gene028530:CDS
TCTCACAGTGAACTCTTATG+AGG 0.440028 4.1:-20515654 None:intergenic
TCTTGAGGAAGAGAGAGTTT+AGG 0.440922 4.1:-20515045 None:intergenic
CTTCAACCCTAGAACTTCAA+TGG 0.444938 4.1:+20514831 MS.gene028530:CDS
CAGTTTCCATTGAAGTTCTA+GGG 0.451054 4.1:-20514837 None:intergenic
TTGAATCAGTTAAATCAGTT+GGG 0.451883 4.1:+20514916 MS.gene028530:CDS
GAGTCTAACTCACTGCCAAT+AGG 0.462111 4.1:+20514760 MS.gene028530:CDS
TCTCCAAAAGCAGTACCTAT+TGG 0.467684 4.1:-20514775 None:intergenic
GAGAATATTTCATTTATCAG+AGG 0.468225 4.1:+20514459 MS.gene028530:CDS
CGACAAATGAAAGAAGATTC+GGG 0.471965 4.1:-20514891 None:intergenic
ACTATATGTCTTACCTGCTC+AGG 0.472883 4.1:-20514486 None:intergenic
TTCTGGCAGTTCCTCGTCAA+AGG 0.474337 4.1:-20515602 None:intergenic
TTTGATCTTGCAGGAAATGA+TGG 0.481839 4.1:+20514690 MS.gene028530:intron
AACCAATCATCTCATCCTTA+GGG 0.484158 4.1:-20514948 None:intergenic
GATTTCTCAAGGAACCTTTG+AGG 0.485480 4.1:+20515002 MS.gene028530:CDS
AAATGTCATGAAACTGCTAT+TGG 0.489746 4.1:-20515737 None:intergenic
AATTCTTCTGCAGAATTTGG+TGG 0.492167 4.1:+20515577 MS.gene028530:CDS
TCTGGCAGTTCCTCGTCAAA+GGG 0.506650 4.1:-20515601 None:intergenic
CTTCATTTCTTCATCATCAG+TGG 0.507118 4.1:+20514719 MS.gene028530:CDS
TCAGTTAAATCAGTTGGGAT+TGG 0.511136 4.1:+20514921 MS.gene028530:CDS
AACCCTAAGGATGAGATGAT+TGG 0.511495 4.1:+20514946 MS.gene028530:CDS
TGATATAACAATTATTGCAC+AGG 0.531379 4.1:+20515774 MS.gene028530:CDS
AGAATATTTCATTTATCAGA+GGG 0.537343 4.1:+20514460 MS.gene028530:CDS
TGAGAAAATCAAAGGAGTTG+TGG 0.539482 4.1:+20515672 MS.gene028530:CDS
CACTGATGATGAAGAAATGA+AGG 0.551145 4.1:-20514718 None:intergenic
TCTTCCGACATGATTTCTCA+AGG 0.551521 4.1:+20514991 MS.gene028530:CDS
TCAGATATTATATGGTCTTG+AGG 0.555208 4.1:-20515060 None:intergenic
GTTGGGATTGGTGAACCCTA+AGG 0.558665 4.1:+20514933 MS.gene028530:CDS
AAGATAGAGCAATGAAGCGC+TGG 0.560225 4.1:-20515107 None:intergenic
GTTCACTGTGAGAAAATCAA+AGG 0.561408 4.1:+20515664 MS.gene028530:CDS
AGTTTGGATAAACGTATGAA+TGG 0.568981 4.1:-20514810 None:intergenic
AGAGAAGGTGAAGGTTCTTG+AGG 0.571434 4.1:+20515480 MS.gene028530:CDS
CTTGAAGAAATTGCAAGAGA+AGG 0.585063 4.1:+20515465 MS.gene028530:CDS
GAAGGTGAAGGTTCTTGAGG+AGG 0.607165 4.1:+20515483 MS.gene028530:CDS
ATATAATATCTGAAAGAAAG+CGG 0.617975 4.1:+20515070 MS.gene028530:CDS
ATTTGGTGGTCCCTTTGACG+AGG 0.618695 4.1:+20515591 MS.gene028530:CDS
AATGAAAGAAGATTCGGGTA+CGG 0.630607 4.1:-20514886 None:intergenic
GATCTTGCAGGAAATGATGG+AGG 0.636854 4.1:+20514693 MS.gene028530:intron
GAAATTGCAAGAGAAGGTGA+AGG 0.642638 4.1:+20515471 MS.gene028530:CDS
GGTTCCTTGAGAAATCATGT+CGG 0.702500 4.1:-20514995 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAGAAGTAATCTAATAA+AGG + chr4.1:20514620-20514639 MS.gene028530:intron 20.0%
!! AATATATAAGAATGTGTTTG+TGG + chr4.1:20515350-20515369 MS.gene028530:intron 20.0%
!! AGAATATTTCATTTATCAGA+GGG + chr4.1:20514460-20514479 MS.gene028530:CDS 20.0%
!! ATATAATATCTGAAAGAAAG+CGG + chr4.1:20515070-20515089 MS.gene028530:CDS 20.0%
!! ATTTAGTGTTTACTATTAGT+AGG + chr4.1:20515317-20515336 MS.gene028530:intron 20.0%
! ACTACAATTGTCTATCAATA+TGG + chr4.1:20515938-20515957 MS.gene028530:intron 25.0%
! CTCTACAAAACAAATTTGTA+AGG + chr4.1:20515209-20515228 MS.gene028530:intron 25.0%
! CTTACTATACATTCATACTT+TGG + chr4.1:20514533-20514552 MS.gene028530:intron 25.0%
! GAGAATATTTCATTTATCAG+AGG + chr4.1:20514459-20514478 MS.gene028530:CDS 25.0%
! GCTTTCTTTCAGATATTATA+TGG - chr4.1:20515071-20515090 None:intergenic 25.0%
! TGATATAACAATTATTGCAC+AGG + chr4.1:20515774-20515793 MS.gene028530:CDS 25.0%
! TTGAATCAGTTAAATCAGTT+GGG + chr4.1:20514916-20514935 MS.gene028530:CDS 25.0%
! TTTGAATCAGTTAAATCAGT+TGG + chr4.1:20514915-20514934 MS.gene028530:CDS 25.0%
!! AGAAGAATTTTCAACATCAT+TGG - chr4.1:20515566-20515585 None:intergenic 25.0%
!! TTTTCTAACCAAATGTTAGA+TGG - chr4.1:20514577-20514596 None:intergenic 25.0%
!! TTTTTATACCTTTTGTAGTC+CGG - chr4.1:20515142-20515161 None:intergenic 25.0%
AAATGTCATGAAACTGCTAT+TGG - chr4.1:20515740-20515759 None:intergenic 30.0%
AATAGCAGTTTCATGACATT+TGG + chr4.1:20515739-20515758 MS.gene028530:CDS 30.0%
AGTTTGGATAAACGTATGAA+TGG - chr4.1:20514813-20514832 None:intergenic 30.0%
ATAGCAGTTTCATGACATTT+GGG + chr4.1:20515740-20515759 MS.gene028530:CDS 30.0%
CAAAATCTTGCTTCAATTTC+TGG - chr4.1:20515622-20515641 None:intergenic 30.0%
GAAAATTCTTCTGCAGAATT+TGG + chr4.1:20515574-20515593 MS.gene028530:CDS 30.0%
GACAATTGTAGTAATTTCGT+TGG - chr4.1:20515930-20515949 None:intergenic 30.0%
GATTGAGTTTCTCAATTTCA+TGG - chr4.1:20515708-20515727 None:intergenic 30.0%
TATCTATGTTGAACACATGT+TGG + chr4.1:20515291-20515310 MS.gene028530:intron 30.0%
TCGACAAATGAAAGAAGATT+CGG - chr4.1:20514895-20514914 None:intergenic 30.0%
TTTACTATTAGTAGGACACA+TGG + chr4.1:20515325-20515344 MS.gene028530:intron 30.0%
! ACATGTTTTCACAATGTTCA+TGG - chr4.1:20514669-20514688 None:intergenic 30.0%
! AGTAATCTAATAAAGGCACA+AGG + chr4.1:20514627-20514646 MS.gene028530:intron 30.0%
! TCAGATATTATATGGTCTTG+AGG - chr4.1:20515063-20515082 None:intergenic 30.0%
! TTTTATACCTTTTGTAGTCC+GGG - chr4.1:20515141-20515160 None:intergenic 30.0%
!!! TTTTGGAACCATCTAACATT+TGG + chr4.1:20514566-20514585 MS.gene028530:intron 30.0%
AAACATGTGTTTGATCTTGC+AGG + chr4.1:20514681-20514700 MS.gene028530:intron 35.0%
AACCAATCATCTCATCCTTA+GGG - chr4.1:20514951-20514970 None:intergenic 35.0%
AATGAAAGAAGATTCGGGTA+CGG - chr4.1:20514889-20514908 None:intergenic 35.0%
AATTCTTCTGCAGAATTTGG+TGG + chr4.1:20515577-20515596 MS.gene028530:CDS 35.0%
ACAGAACATGAAGAAAAACG+TGG + chr4.1:20515507-20515526 MS.gene028530:CDS 35.0%
ATGGACAAAGTTACTGTTCT+TGG + chr4.1:20515427-20515446 MS.gene028530:CDS 35.0%
CAGTTTCCATTGAAGTTCTA+GGG - chr4.1:20514840-20514859 None:intergenic 35.0%
CGACAAATGAAAGAAGATTC+GGG - chr4.1:20514894-20514913 None:intergenic 35.0%
CTATTTGTGCAATTTGCAGA+TGG + chr4.1:20515964-20515983 MS.gene028530:intron 35.0%
CTTCATTTCTTCATCATCAG+TGG + chr4.1:20514719-20514738 MS.gene028530:CDS 35.0%
CTTGAAGAAATTGCAAGAGA+AGG + chr4.1:20515465-20515484 MS.gene028530:CDS 35.0%
GAAGAAAAACGTGGAATTTG+TGG + chr4.1:20515516-20515535 MS.gene028530:CDS 35.0%
GTTCACTGTGAGAAAATCAA+AGG + chr4.1:20515664-20515683 MS.gene028530:CDS 35.0%
TCAGTTAAATCAGTTGGGAT+TGG + chr4.1:20514921-20514940 MS.gene028530:CDS 35.0%
TGAGAAAATCAAAGGAGTTG+TGG + chr4.1:20515672-20515691 MS.gene028530:CDS 35.0%
TGTAAGGTGAAGATTGCATT+AGG + chr4.1:20515225-20515244 MS.gene028530:intron 35.0%
TTTGATCTTGCAGGAAATGA+TGG + chr4.1:20514690-20514709 MS.gene028530:intron 35.0%
! ACTTTGGCAGTGACTAATTT+TGG + chr4.1:20514549-20514568 MS.gene028530:intron 35.0%
! ATTGTGCTACTACTAACAGA+TGG + chr4.1:20515408-20515427 MS.gene028530:intron 35.0%
! CACTGATGATGAAGAAATGA+AGG - chr4.1:20514721-20514740 None:intergenic 35.0%
! GGATATGGAATTTTGCATGA+CGG + chr4.1:20515985-20516004 MS.gene028530:CDS 35.0%
!! TTGTTTTGTAGAGTTGAGTC+AGG - chr4.1:20515202-20515221 None:intergenic 35.0%
AAAAACTTAAGTCCCGCGTT+AGG - chr4.1:20515265-20515284 None:intergenic 40.0%
AACCCTAAGGATGAGATGAT+TGG + chr4.1:20514946-20514965 MS.gene028530:CDS 40.0%
AAGACGTAAAAACTGAGCGT+AGG - chr4.1:20516030-20516049 None:intergenic 40.0%
ACTATATGTCTTACCTGCTC+AGG - chr4.1:20514489-20514508 None:intergenic 40.0%
CTTCAACCCTAGAACTTCAA+TGG + chr4.1:20514831-20514850 MS.gene028530:CDS 40.0%
GAAAAACGTGGAATTTGTGG+TGG + chr4.1:20515519-20515538 MS.gene028530:CDS 40.0%
GAAATTGCAAGAGAAGGTGA+AGG + chr4.1:20515471-20515490 MS.gene028530:CDS 40.0%
GAACCAATCATCTCATCCTT+AGG - chr4.1:20514952-20514971 None:intergenic 40.0%
GATTTCTCAAGGAACCTTTG+AGG + chr4.1:20515002-20515021 MS.gene028530:CDS 40.0%
GCAGTTTCCATTGAAGTTCT+AGG - chr4.1:20514841-20514860 None:intergenic 40.0%
GGTTCCTTGAGAAATCATGT+CGG - chr4.1:20514998-20515017 None:intergenic 40.0%
GTGCAATTTGCAGATGGATA+TGG + chr4.1:20515970-20515989 MS.gene028530:intron 40.0%
TCTCACAGTGAACTCTTATG+AGG - chr4.1:20515657-20515676 None:intergenic 40.0%
TCTCCAAAAGCAGTACCTAT+TGG - chr4.1:20514778-20514797 None:intergenic 40.0%
TCTTCCGACATGATTTCTCA+AGG + chr4.1:20514991-20515010 MS.gene028530:CDS 40.0%
TCTTGAGGAAGAGAGAGTTT+AGG - chr4.1:20515048-20515067 None:intergenic 40.0%
TTTATGATCTCTGCCTAACG+CGG + chr4.1:20515249-20515268 MS.gene028530:intron 40.0%
!! GAAGTTCTAGGGTTGAAGTT+TGG - chr4.1:20514829-20514848 None:intergenic 40.0%
AAGATAGAGCAATGAAGCGC+TGG - chr4.1:20515110-20515129 None:intergenic 45.0%
AGAGAAGGTGAAGGTTCTTG+AGG + chr4.1:20515480-20515499 MS.gene028530:CDS 45.0%
GAGTCTAACTCACTGCCAAT+AGG + chr4.1:20514760-20514779 MS.gene028530:CDS 45.0%
GATCTTGCAGGAAATGATGG+AGG + chr4.1:20514693-20514712 MS.gene028530:intron 45.0%
TTATGATCTCTGCCTAACGC+GGG + chr4.1:20515250-20515269 MS.gene028530:intron 45.0%
! CTTTTGTAGTCCGGGAACAA+GGG - chr4.1:20515133-20515152 None:intergenic 45.0%
! GGAATTTTGCATGACGGTGA+AGG + chr4.1:20515991-20516010 MS.gene028530:CDS 45.0%
!! AGGAACCTTTGAGGCCAAAA+AGG + chr4.1:20515011-20515030 MS.gene028530:CDS 45.0%
!! AGGACGTGTTGCTACCTTTT+TGG - chr4.1:20515028-20515047 None:intergenic 45.0%
!! CTGCCAATAGGTACTGCTTT+TGG + chr4.1:20514772-20514791 MS.gene028530:CDS 45.0%
!! TGCTACCTTTTTGGCCTCAA+AGG - chr4.1:20515019-20515038 None:intergenic 45.0%
CCTTGTTCCCGGACTACAAA+AGG + chr4.1:20515131-20515150 MS.gene028530:CDS 50.0%
GAAGGTGAAGGTTCTTGAGG+AGG + chr4.1:20515483-20515502 MS.gene028530:CDS 50.0%
GCTCTATCTTCCCTTGTTCC+CGG + chr4.1:20515120-20515139 MS.gene028530:CDS 50.0%
TATCAGAGGGTTTCCTGAGC+AGG + chr4.1:20514473-20514492 MS.gene028530:CDS 50.0%
TCTGGCAGTTCCTCGTCAAA+GGG - chr4.1:20515604-20515623 None:intergenic 50.0%
TTCTGGCAGTTCCTCGTCAA+AGG - chr4.1:20515605-20515624 None:intergenic 50.0%
! ATTTGGTGGTCCCTTTGACG+AGG + chr4.1:20515591-20515610 MS.gene028530:CDS 50.0%
! CCTTTTGTAGTCCGGGAACA+AGG - chr4.1:20515134-20515153 None:intergenic 50.0%
! GTTGGGATTGGTGAACCCTA+AGG + chr4.1:20514933-20514952 MS.gene028530:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 20514456 20516057 20514456 ID=MS.gene028530
chr4.1 mRNA 20514456 20516057 20514456 ID=MS.gene028530.t1;Parent=MS.gene028530
chr4.1 exon 20514456 20514494 20514456 ID=MS.gene028530.t1.exon1;Parent=MS.gene028530.t1
chr4.1 CDS 20514456 20514494 20514456 ID=cds.MS.gene028530.t1;Parent=MS.gene028530.t1
chr4.1 exon 20514703 20515152 20514703 ID=MS.gene028530.t1.exon2;Parent=MS.gene028530.t1
chr4.1 CDS 20514703 20515152 20514703 ID=cds.MS.gene028530.t1;Parent=MS.gene028530.t1
chr4.1 exon 20515427 20515795 20515427 ID=MS.gene028530.t1.exon3;Parent=MS.gene028530.t1
chr4.1 CDS 20515427 20515795 20515427 ID=cds.MS.gene028530.t1;Parent=MS.gene028530.t1
chr4.1 exon 20515983 20516057 20515983 ID=MS.gene028530.t1.exon4;Parent=MS.gene028530.t1
chr4.1 CDS 20515983 20516057 20515983 ID=cds.MS.gene028530.t1;Parent=MS.gene028530.t1
Gene Sequence

>MS.gene028530

ATGGAGAATATTTCATTTATCAGAGGGTTTCCTGAGCAGGTAAGACATATAGTTCTTGCAAATAGTTTTTCTCTGAACTTACTATACATTCATACTTTGGCAGTGACTAATTTTGGAACCATCTAACATTTGGTTAGAAAATAAGACAAGTATCTAATAATAAAAAAGAGAAGTAATCTAATAAAGGCACAAGGTAAATGCATGAAATTTCCATGAACATTGTGAAAACATGTGTTTGATCTTGCAGGAAATGATGGAGGATCCTTCATTTCTTCATCATCAGTGGCATTTGAGCTCTATCAATGAGTCTAACTCACTGCCAATAGGTACTGCTTTTGGAGACACTTCACAACACCATTCATACGTTTATCCAAACTTCAACCCTAGAACTTCAATGGAAACTGCTCAGACACTAGAGACTCAATTTGTTCCGTACCCGAATCTTCTTTCATTTGTCGATTTGAATCAGTTAAATCAGTTGGGATTGGTGAACCCTAAGGATGAGATGATTGGTTCTCAAAACAACAACACAACTTCTTCCGACATGATTTCTCAAGGAACCTTTGAGGCCAAAAAGGTAGCAACACGTCCTAAACTCTCTCTTCCTCAAGACCATATAATATCTGAAAGAAAGCGGCGTGAGAAGCTTAGCCAGCGCTTCATTGCTCTATCTTCCCTTGTTCCCGGACTACAAAAGGTATAAAAAATTCGACTATCACGAATTTTTTACATTTTATTTTGAACCTGACTCAACTCTACAAAACAAATTTGTAAGGTGAAGATTGCATTAGGCTTTATGATCTCTGCCTAACGCGGGACTTAAGTTTTTTCTCGATATCTATGTTGAACACATGTTGGATCATTTAGTGTTTACTATTAGTAGGACACATGGTGAATATATAAGAATGTGTTTGTGGTACTATCTTTAACAATTTTTGTCGTCTCGTTTTTCATTGTGCTACTACTAACAGATGGACAAAGTTACTGTTCTTGGAGATGCTATCAAGTACTTGAAGAAATTGCAAGAGAAGGTGAAGGTTCTTGAGGAGGAACAGAACATGAAGAAAAACGTGGAATTTGTGGTGGTTGTGAAGAAATATCACTTATCCAATGATGTTGAAAATTCTTCTGCAGAATTTGGTGGTCCCTTTGACGAGGAACTGCCAGAAATTGAAGCAAGATTTTGTGATAGAAATGTCCTCATAAGAGTTCACTGTGAGAAAATCAAAGGAGTTGTGGAAAAAACAATCCATGAAATTGAGAAACTCAATCTAAAAGTCACCAATAGCAGTTTCATGACATTTGGGAGTTGTGCACTTGATATAACAATTATTGCACAGGTAATCTTTGTTTCAACAAAAGTATTGAATTAAGTTTCAGTAGTTATATTTTAAGACTTTTGACTACTTTAATGAAAAAACTTGCTTATTTACTGTATATCTATCTATGCATAATTTTCTATTATTTTGTTCCAACGAAATTACTACAATTGTCTATCAATATGGAAACTATTTGTGCAATTTGCAGATGGATATGGAATTTTGCATGACGGTGAAGGATCTTGTGAGAAACCTACGCTCAGTTTTTACGTCTTTCATCTAA
Protein sequence

>MS.gene028530.t1

MENISFIRGFPEQEMMEDPSFLHHQWHLSSINESNSLPIGTAFGDTSQHHSYVYPNFNPRTSMETAQTLETQFVPYPNLLSFVDLNQLNQLGLVNPKDEMIGSQNNNTTSSDMISQGTFEAKKVATRPKLSLPQDHIISERKRREKLSQRFIALSSLVPGLQKMDKVTVLGDAIKYLKKLQEKVKVLEEEQNMKKNVEFVVVVKKYHLSNDVENSSAEFGGPFDEELPEIEARFCDRNVLIRVHCEKIKGVVEKTIHEIEKLNLKVTNSSFMTFGSCALDITIIAQMDMEFCMTVKDLVRNLRSVFTSFI