AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene029220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029220.t1 MTR_1g114290 93.976 166 7 2 1 165 1 164 5.07e-103 293
MS.gene029220.t1 MTR_2g079420 37.607 117 73 0 37 153 36 152 4.37e-25 95.5
MS.gene029220.t1 MTR_3g089065 42.169 83 48 0 71 153 70 152 7.00e-24 92.4
MS.gene029220.t1 MTR_4g085880 45.122 82 45 0 72 153 66 147 2.90e-23 90.9
MS.gene029220.t1 MTR_1g098660 40.909 88 50 1 77 164 68 153 2.57e-22 88.2
MS.gene029220.t1 MTR_8g059015 39.759 83 50 0 71 153 73 155 1.04e-20 84.3
MS.gene029220.t1 MTR_7g009070 39.744 78 43 2 86 160 47 123 7.17e-17 73.2
MS.gene029220.t1 MTR_2g079400 37.209 86 54 0 68 153 31 116 1.42e-15 69.7
MS.gene029220.t1 MTR_5g084930 33.607 122 76 2 53 171 14 133 5.60e-15 72.0
MS.gene029220.t1 MTR_5g084930 32.323 99 63 1 73 167 162 260 1.87e-13 67.4
MS.gene029220.t1 MTR_2g079340 38.750 80 49 0 74 153 39 118 1.07e-14 68.2
MS.gene029220.t1 MTR_1g023140 35.238 105 58 4 77 172 15 118 3.86e-14 65.9
MS.gene029220.t1 MTR_2g079380 38.372 86 53 0 68 153 15 100 1.20e-13 67.0
MS.gene029220.t1 MTR_2g021750 35.043 117 71 2 53 166 14 128 1.85e-13 67.4
MS.gene029220.t1 MTR_1g087520 39.535 86 46 2 79 158 157 242 1.85e-13 67.4
MS.gene029220.t1 MTR_1g037680 35.294 85 53 1 81 163 89 173 5.29e-13 64.3
MS.gene029220.t1 MTR_3g112410 35.955 89 50 3 86 168 27 114 6.62e-13 62.4
MS.gene029220.t1 MTR_2g079360 34.375 96 62 1 59 153 34 129 2.61e-12 61.6
MS.gene029220.t1 MTR_5g021180 27.473 91 61 3 85 172 28 116 3.11e-11 58.2
MS.gene029220.t1 MTR_8g107460 35.294 85 53 2 70 153 258 341 5.38e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene029220.t1 AT1G50320 53.757 173 64 5 1 165 1 165 9.56e-55 171
MS.gene029220.t1 AT1G76760 44.186 86 46 1 79 164 73 156 3.58e-24 93.2
MS.gene029220.t1 AT1G43560 43.529 85 46 1 79 163 68 150 8.40e-22 87.0
MS.gene029220.t1 AT1G03680 40.476 84 50 0 70 153 75 158 3.07e-21 85.9
MS.gene029220.t1 AT2G15570 41.463 82 48 0 72 153 69 150 8.45e-21 84.3
MS.gene029220.t1 AT2G15570 41.463 82 48 0 72 153 70 151 8.46e-21 84.3
MS.gene029220.t1 AT3G15360 39.759 83 50 0 71 153 88 170 1.87e-20 84.0
MS.gene029220.t1 AT4G03520 37.349 83 52 0 71 153 82 164 2.90e-18 78.2
MS.gene029220.t1 AT5G39950 37.209 86 49 3 78 160 39 122 9.83e-16 70.1
MS.gene029220.t1 AT2G32920 35.577 104 64 1 71 171 32 135 1.30e-15 73.9
MS.gene029220.t1 AT2G32920 28.846 104 70 1 72 171 165 268 8.92e-12 62.8
MS.gene029220.t1 AT1G04980 36.538 104 63 1 71 171 34 137 1.99e-15 73.2
MS.gene029220.t1 AT1G04980 31.633 98 63 1 73 166 171 268 1.33e-11 62.4
MS.gene029220.t1 AT2G47470 39.773 88 47 2 77 158 149 236 1.94e-13 67.4
MS.gene029220.t1 AT2G47470 39.773 88 47 2 77 158 149 236 2.00e-13 67.0
MS.gene029220.t1 AT2G47470 39.773 88 47 2 77 158 149 236 2.06e-13 66.6
MS.gene029220.t1 AT2G47470 39.773 88 47 2 77 158 149 236 2.46e-13 67.0
MS.gene029220.t1 AT5G60640 36.275 102 61 3 74 172 108 208 4.98e-13 66.2
MS.gene029220.t1 AT3G06730 34.737 95 59 2 71 163 77 170 6.28e-13 63.9
MS.gene029220.t1 AT5G42980 38.235 68 41 1 85 152 25 91 1.22e-12 61.6
MS.gene029220.t1 AT1G77510 36.634 101 53 5 74 164 34 133 1.32e-12 65.1
MS.gene029220.t1 AT5G60640 37.755 98 56 3 71 164 105 201 3.46e-12 63.9
MS.gene029220.t1 AT5G60640 37.755 98 56 3 71 164 105 201 1.12e-11 62.4
MS.gene029220.t1 AT1G69880 35.714 84 52 2 71 153 42 124 1.91e-11 59.3
MS.gene029220.t1 AT1G19730 30.380 79 52 1 85 160 26 104 4.36e-11 57.8
MS.gene029220.t1 AT1G45145 29.070 86 59 2 66 151 7 90 7.14e-11 57.0

Find 49 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAAGAGTATAAAGTTTA+TGG 0.088645 1.3:-77428463 MS.gene029220:CDS
CCCCGTTCTTGAAGAGTATC+AGG 0.263376 1.3:+77428431 None:intergenic
GCACCTTCCTTCCTGCTTCC+TGG 0.279114 1.3:+77428400 None:intergenic
CCCTGATACTCTTCAAGAAC+GGG 0.388833 1.3:-77428432 MS.gene029220:CDS
TCAATACGGTTTCCTTGAAC+TGG 0.391435 1.3:+77430140 None:intergenic
AAGAACGGGGAGGAATTTCC+AGG 0.409346 1.3:-77428418 MS.gene029220:CDS
TCTAACTCTCCCAACCGTTC+CGG 0.449468 1.3:-77430335 MS.gene029220:CDS
CCGTTTGATCACTCCAGCTA+TGG 0.459141 1.3:-77430065 MS.gene029220:CDS
TAGTTGAATTCGTTGCTACT+TGG 0.466999 1.3:-77430099 MS.gene029220:CDS
ATTGTACGAACCGGAACGGT+TGG 0.469903 1.3:+77430325 None:intergenic
TTGTACGAACCGGAACGGTT+GGG 0.477965 1.3:+77430326 None:intergenic
TGAGCTATGGATTCCATAGC+TGG 0.482178 1.3:+77430052 None:intergenic
AGAAAGAGGTACCTGAGCTA+TGG 0.486986 1.3:+77430039 None:intergenic
GCAACGAATTCAACTAAAAC+CGG 0.491722 1.3:+77430106 None:intergenic
GTTGAGGTCACCGTATCCAT+TGG 0.493134 1.3:+77430373 None:intergenic
CGTATTGAAATCTGAGCGTC+CGG 0.497098 1.3:-77430125 MS.gene029220:CDS
TAAGGGAAGAGATGAGGATA+CGG 0.499243 1.3:+77430288 None:intergenic
TGACCGGTGAGAACGTGGTA+AGG 0.505991 1.3:+77430270 None:intergenic
AGAGTCTTTGCCGAGTTGAC+CGG 0.508220 1.3:+77430254 None:intergenic
TATACTCTTCAATTAGCTGC+GGG 0.511786 1.3:+77428473 None:intergenic
GGGAGGAATTTCCAGGAAGC+AGG 0.512537 1.3:-77428411 MS.gene029220:CDS
CCGCAACTCACTGTAAACTT+AGG 0.512897 1.3:+77430184 None:intergenic
AATCAATGAAAGCCAGTTCA+AGG 0.512971 1.3:-77430152 MS.gene029220:CDS
TTCCCTTACCACGTTCTCAC+CGG 0.519028 1.3:-77430273 MS.gene029220:CDS
TACAGTGAGTTGCGGTATCA+CGG 0.521048 1.3:-77430176 MS.gene029220:CDS
AGCTATGGAATCCATAGCTC+AGG 0.524162 1.3:-77430050 MS.gene029220:intron
CACGTTCTCACCGGTCAACT+CGG 0.532357 1.3:-77430264 MS.gene029220:CDS
TGAGGATACGGAGATTGAAG+AGG 0.544293 1.3:+77430300 None:intergenic
CGGACGCTCAGATTTCAATA+CGG 0.547344 1.3:+77430126 None:intergenic
CCATAGCTGGAGTGATCAAA+CGG 0.549337 1.3:+77430065 None:intergenic
GAAGAGGTAATTGTACGAAC+CGG 0.550130 1.3:+77430316 None:intergenic
CCTAAGTTTACAGTGAGTTG+CGG 0.570949 1.3:-77430184 MS.gene029220:CDS
TCATTGAAATCCAATGGATA+CGG 0.572041 1.3:-77430383 None:intergenic
ACGTGGTAAGGGAAGAGATG+AGG 0.572348 1.3:+77430282 None:intergenic
ACCCTGATACTCTTCAAGAA+CGG 0.577296 1.3:-77428433 MS.gene029220:CDS
TTCTTGAAGAGTATCAGGGT+TGG 0.579923 1.3:+77428436 None:intergenic
CCCGTTCTTGAAGAGTATCA+GGG 0.580271 1.3:+77428432 None:intergenic
TTTCCAGGAAGCAGGAAGGA+AGG 0.591083 1.3:-77428403 MS.gene029220:CDS
GACCGGTGAGAACGTGGTAA+GGG 0.599839 1.3:+77430271 None:intergenic
CGAGTTGACCGGTGAGAACG+TGG 0.605237 1.3:+77430265 None:intergenic
GAATTCGTTGCTACTTGGTG+CGG 0.606167 1.3:-77430094 MS.gene029220:CDS
TTATACTCTTCAATTAGCTG+CGG 0.611346 1.3:+77428472 None:intergenic
AGTGAGCGAAGCTGAAGTTG+AGG 0.632954 1.3:+77430357 None:intergenic
GGAATTTCCAGGAAGCAGGA+AGG 0.646284 1.3:-77428407 MS.gene029220:CDS
GGTAATTGTACGAACCGGAA+CGG 0.669877 1.3:+77430321 None:intergenic
GAAGGAAGGTGCAATTACAA+AGG 0.680704 1.3:-77428389 MS.gene029220:intron
GCTGGAGTGATCAAACGGCA+AGG 0.683277 1.3:+77430070 None:intergenic
CCTGATACTCTTCAAGAACG+GGG 0.694186 1.3:-77428431 MS.gene029220:CDS
GATACTCTTCAAGAACGGGG+AGG 0.730377 1.3:-77428428 MS.gene029220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATAGATAGTTATTG+AGG - chr1.3:77429656-77429675 MS.gene029220:intron 15.0%
!! GTACAATTTCAAATTAAAAT+CGG - chr1.3:77430256-77430275 MS.gene029220:CDS 15.0%
!!! CTTCAATTTTATTAAGAAAA+AGG - chr1.3:77429352-77429371 MS.gene029220:intron 15.0%
!! GTATATATTATACACTTCAA+AGG + chr1.3:77430232-77430251 None:intergenic 20.0%
!! TTTCAAATTATTAGTTGTGT+CGG - chr1.3:77429014-77429033 MS.gene029220:intron 20.0%
!!! ACCTCTAAAATCATTTATAT+TGG - chr1.3:77428555-77428574 MS.gene029220:intron 20.0%
!!! ACTTTTTATTTTGTTTTTGC+CGG - chr1.3:77428368-77428387 MS.gene029220:intron 20.0%
!!! ATTGAAGAGTATAAAGTTTA+TGG - chr1.3:77429912-77429931 MS.gene029220:intron 20.0%
!!! CCTCTAAAATCATTTATATT+GGG - chr1.3:77428556-77428575 MS.gene029220:intron 20.0%
! ATTGATATGCTAAATGAAAC+AGG + chr1.3:77430127-77430146 None:intergenic 25.0%
! TGAAAATTATGTGATCTTTC+AGG - chr1.3:77428800-77428819 MS.gene029220:intron 25.0%
! TTAAATCAGACCATCAATTT+GGG + chr1.3:77429402-77429421 None:intergenic 25.0%
!! AATGTAAATTTTTGTGTGTG+GGG - chr1.3:77428673-77428692 MS.gene029220:intron 25.0%
!! ACAATGTAAATTTTTGTGTG+TGG - chr1.3:77428671-77428690 MS.gene029220:intron 25.0%
!! CAATGTAAATTTTTGTGTGT+GGG - chr1.3:77428672-77428691 MS.gene029220:intron 25.0%
!! CCCAATATAAATGATTTTAG+AGG + chr1.3:77428559-77428578 None:intergenic 25.0%
!! TATTACTAGTTTCCCAAAAA+TGG + chr1.3:77430094-77430113 None:intergenic 25.0%
!!! CTTTAATTTGCAAATGATGT+CGG + chr1.3:77429704-77429723 None:intergenic 25.0%
!!! CTTTTTTTAAACTCAGTATC+CGG + chr1.3:77428390-77428409 None:intergenic 25.0%
!!! GTTAGTCGTTTATCTTTTTT+AGG - chr1.3:77429577-77429596 MS.gene029220:intron 25.0%
!!! TTTTGGGAAACTAGTAATAT+GGG - chr1.3:77430095-77430114 MS.gene029220:CDS 25.0%
!!! TTTTTGGGAAACTAGTAATA+TGG - chr1.3:77430094-77430113 MS.gene029220:CDS 25.0%
AAAGTTAGAAAGAGAGAAAG+AGG + chr1.3:77428353-77428372 None:intergenic 30.0%
AAATCATTTATATTGGGCTG+CGG - chr1.3:77428562-77428581 MS.gene029220:intron 30.0%
AACAAAATGTCTTAAGCTTC+TGG - chr1.3:77428888-77428907 MS.gene029220:intron 30.0%
ACAACATTTGGATGCTTTAT+TGG - chr1.3:77430019-77430038 MS.gene029220:intron 30.0%
ACTCTCATACATTATATTGC+AGG - chr1.3:77429833-77429852 MS.gene029220:intron 30.0%
AGCTATTCTTAAACGTTCTT+TGG - chr1.3:77429786-77429805 MS.gene029220:intron 30.0%
ATTATCATTCATACGAATGC+AGG - chr1.3:77429607-77429626 MS.gene029220:intron 30.0%
CATGCAATTCACTGTTTAAT+TGG - chr1.3:77428453-77428472 MS.gene029220:CDS 30.0%
CTTAAATCAGACCATCAATT+TGG + chr1.3:77429403-77429422 None:intergenic 30.0%
GCTATTCTTAAACGTTCTTT+GGG - chr1.3:77429787-77429806 MS.gene029220:intron 30.0%
GGTGAAACTTAAACAACATT+TGG - chr1.3:77430007-77430026 MS.gene029220:intron 30.0%
TAGTCTACAATCATACTACA+AGG + chr1.3:77428842-77428861 None:intergenic 30.0%
TTATACTCTTCAATTAGCTG+CGG + chr1.3:77429906-77429925 None:intergenic 30.0%
TTCAGTCTAATTACAAGTGT+CGG + chr1.3:77428983-77429002 None:intergenic 30.0%
! AGACTATAACACCTCTTTTT+GGG - chr1.3:77428623-77428642 MS.gene029220:intron 30.0%
! ATGTAAATTTTTGTGTGTGG+GGG - chr1.3:77428674-77428693 MS.gene029220:intron 30.0%
! ATGTTTCTATCTTCGATTCA+TGG - chr1.3:77428712-77428731 MS.gene029220:intron 30.0%
! CAAATTGATGGTCTGATTTA+AGG - chr1.3:77429401-77429420 MS.gene029220:intron 30.0%
! TAGACTATAACACCTCTTTT+TGG - chr1.3:77428622-77428641 MS.gene029220:intron 30.0%
! TGATATGATATTACTAGCCT+AGG - chr1.3:77429763-77429782 MS.gene029220:intron 30.0%
! TTCCAAATTGTGACCATTTT+TGG - chr1.3:77430078-77430097 MS.gene029220:CDS 30.0%
!! TCCAAATTGTGACCATTTTT+GGG - chr1.3:77430079-77430098 MS.gene029220:CDS 30.0%
!!! CTCACTTGTTTTTTGTACTA+AGG - chr1.3:77430305-77430324 MS.gene029220:CDS 30.0%
AAATCGGTCTGATCTGTTTA+TGG - chr1.3:77430272-77430291 MS.gene029220:CDS 35.0%
AACGTTTAAGAATAGCTCCT+AGG + chr1.3:77429783-77429802 None:intergenic 35.0%
AATCAATGAAAGCCAGTTCA+AGG - chr1.3:77428223-77428242 MS.gene029220:intron 35.0%
ACGTTAGAAGAATGACTTTG+AGG - chr1.3:77429125-77429144 MS.gene029220:intron 35.0%
AGTACTAATAAGCCTCTGTT+TGG + chr1.3:77429550-77429569 None:intergenic 35.0%
CTGTGCGTAGATTTAAATTG+CGG - chr1.3:77428483-77428502 MS.gene029220:CDS 35.0%
GCAACGAATTCAACTAAAAC+CGG + chr1.3:77428272-77428291 None:intergenic 35.0%
TAATAAGCCTCTGTTTGGTA+TGG + chr1.3:77429545-77429564 None:intergenic 35.0%
TAGTTGAATTCGTTGCTACT+TGG - chr1.3:77428276-77428295 MS.gene029220:intron 35.0%
TATACTCTTCAATTAGCTGC+GGG + chr1.3:77429905-77429924 None:intergenic 35.0%
TCCCAAAAATGGTCACAATT+TGG + chr1.3:77430083-77430102 None:intergenic 35.0%
TTGTCCAAAACCCAAATTGA+TGG - chr1.3:77429389-77429408 MS.gene029220:intron 35.0%
! GACTATAACACCTCTTTTTG+GGG - chr1.3:77428624-77428643 MS.gene029220:intron 35.0%
! GCAAATTCAACAGCATTTTG+CGG + chr1.3:77428525-77428544 None:intergenic 35.0%
!!! AATTTGGGTTTTGGACAAGA+GGG + chr1.3:77429387-77429406 None:intergenic 35.0%
AAGCCTATGAAACACGGATA+CGG + chr1.3:77429075-77429094 None:intergenic 40.0%
AAGTCATTCTTCTAACGTCG+CGG + chr1.3:77429123-77429142 None:intergenic 40.0%
ACCCTGATACTCTTCAAGAA+CGG - chr1.3:77429942-77429961 MS.gene029220:intron 40.0%
CCTAAGTTTACAGTGAGTTG+CGG - chr1.3:77428191-77428210 MS.gene029220:intron 40.0%
GAAGAGGTAATTGTACGAAC+CGG + chr1.3:77428062-77428081 None:intergenic 40.0%
GGTGTGTTAGCATTCATGAT+AGG - chr1.3:77429422-77429441 MS.gene029220:intron 40.0%
TAAGGGAAGAGATGAGGATA+CGG + chr1.3:77428090-77428109 None:intergenic 40.0%
TAGCATTCATGATAGGATGC+CGG - chr1.3:77429429-77429448 MS.gene029220:intron 40.0%
TCAATACGGTTTCCTTGAAC+TGG + chr1.3:77428238-77428257 None:intergenic 40.0%
TCTTCGATTCATGGTACATG+TGG - chr1.3:77428721-77428740 MS.gene029220:intron 40.0%
TGCTGTTGAATTTGCAGTAG+TGG - chr1.3:77428530-77428549 MS.gene029220:intron 40.0%
TTATGCAACACCCCAAAAAG+AGG + chr1.3:77428637-77428656 None:intergenic 40.0%
! GAAGGAAGGTGCAATTACAA+AGG - chr1.3:77429986-77430005 MS.gene029220:intron 40.0%
!! CAGACCATCAATTTGGGTTT+TGG + chr1.3:77429396-77429415 None:intergenic 40.0%
!! GAATGACACAGTTTTGTAGC+AGG - chr1.3:77430358-77430377 MS.gene029220:CDS 40.0%
!! GCAAATGATGTCGGCTTTAA+GGG + chr1.3:77429695-77429714 None:intergenic 40.0%
!! GCTTTAAGGGTAGAATCTCA+GGG + chr1.3:77429682-77429701 None:intergenic 40.0%
!! TGCAAATGATGTCGGCTTTA+AGG + chr1.3:77429696-77429715 None:intergenic 40.0%
!! TTCTTGAAGAGTATCAGGGT+TGG + chr1.3:77429942-77429961 None:intergenic 40.0%
!!! CAATTTGGGTTTTGGACAAG+AGG + chr1.3:77429388-77429407 None:intergenic 40.0%
ACATGTGGATTTCACTCGAC+AGG - chr1.3:77428736-77428755 MS.gene029220:intron 45.0%
AGCATTCATGATAGGATGCC+GGG - chr1.3:77429430-77429449 MS.gene029220:intron 45.0%
AGCTATGGAATCCATAGCTC+AGG - chr1.3:77428325-77428344 MS.gene029220:intron 45.0%
ATCCGTGTTTCATAGGCTTC+TGG - chr1.3:77429076-77429095 MS.gene029220:intron 45.0%
ATGAAACACGGATACGGACA+TGG + chr1.3:77429069-77429088 None:intergenic 45.0%
ATTAGCGTTTGCTTGCGAGT+TGG - chr1.3:77429192-77429211 MS.gene029220:intron 45.0%
CAAATCAACACACAGTGACC+CGG + chr1.3:77429451-77429470 None:intergenic 45.0%
CACCAGAAGCCTATGAAACA+CGG + chr1.3:77429081-77429100 None:intergenic 45.0%
CCATAGCTGGAGTGATCAAA+CGG + chr1.3:77428313-77428332 None:intergenic 45.0%
CCCTGATACTCTTCAAGAAC+GGG - chr1.3:77429943-77429962 MS.gene029220:intron 45.0%
CCGCAACTCACTGTAAACTT+AGG + chr1.3:77428194-77428213 None:intergenic 45.0%
CCTGATACTCTTCAAGAACG+GGG - chr1.3:77429944-77429963 MS.gene029220:intron 45.0%
CGGACGCTCAGATTTCAATA+CGG + chr1.3:77428252-77428271 None:intergenic 45.0%
CGTATTGAAATCTGAGCGTC+CGG - chr1.3:77428250-77428269 MS.gene029220:intron 45.0%
GGTAATTGTACGAACCGGAA+CGG + chr1.3:77428057-77428076 None:intergenic 45.0%
TACAGTGAGTTGCGGTATCA+CGG - chr1.3:77428199-77428218 MS.gene029220:intron 45.0%
TGAGCTATGGATTCCATAGC+TGG + chr1.3:77428326-77428345 None:intergenic 45.0%
TGAGGATACGGAGATTGAAG+AGG + chr1.3:77428078-77428097 None:intergenic 45.0%
TGTCCGTATCCGTGTTTCAT+AGG - chr1.3:77429069-77429088 MS.gene029220:intron 45.0%
TTAGCGTTTGCTTGCGAGTT+GGG - chr1.3:77429193-77429212 MS.gene029220:intron 45.0%
TTTGAAGGGAGGACTTTGGA+GGG - chr1.3:77429217-77429236 MS.gene029220:intron 45.0%
! AAGCTTCTGGCTATGAAGCA+CGG - chr1.3:77428901-77428920 MS.gene029220:intron 45.0%
! GAATTCGTTGCTACTTGGTG+CGG - chr1.3:77428281-77428300 MS.gene029220:intron 45.0%
!! AGAAAGAGGTACCTGAGCTA+TGG + chr1.3:77428339-77428358 None:intergenic 45.0%
!! CCCGTTCTTGAAGAGTATCA+GGG + chr1.3:77429946-77429965 None:intergenic 45.0%
!! CTTTCAGGTGCTGCTTTGAT+TGG - chr1.3:77428815-77428834 MS.gene029220:intron 45.0%
!! GGCTTTAAGGGTAGAATCTC+AGG + chr1.3:77429683-77429702 None:intergenic 45.0%
!! GGGGTGTTGCATAAGACAAT+AGG - chr1.3:77428643-77428662 MS.gene029220:intron 45.0%
!! TTTTGAAGGGAGGACTTTGG+AGG - chr1.3:77429216-77429235 MS.gene029220:intron 45.0%
!!! CTTGCGAGTTGGGTTTTGAA+GGG - chr1.3:77429203-77429222 MS.gene029220:intron 45.0%
!!! ATTTGATATTTTTAAAAAAT+TGG - chr1.3:77429260-77429279 MS.gene029220:intron 5.0%
AAGAACGGGGAGGAATTTCC+AGG - chr1.3:77429957-77429976 MS.gene029220:intron 50.0%
ACGGACACTCCTCGTATTAG+GGG - chr1.3:77428920-77428939 MS.gene029220:intron 50.0%
ACGTGGTAAGGGAAGAGATG+AGG + chr1.3:77428096-77428115 None:intergenic 50.0%
AGTGAGCGAAGCTGAAGTTG+AGG + chr1.3:77428021-77428040 None:intergenic 50.0%
ATTGTACGAACCGGAACGGT+TGG + chr1.3:77428053-77428072 None:intergenic 50.0%
CACGGACACTCCTCGTATTA+GGG - chr1.3:77428919-77428938 MS.gene029220:intron 50.0%
CCGTTTGATCACTCCAGCTA+TGG - chr1.3:77428310-77428329 MS.gene029220:intron 50.0%
GATACTCTTCAAGAACGGGG+AGG - chr1.3:77429947-77429966 MS.gene029220:intron 50.0%
GCTACTGCCATACCAAACAG+AGG - chr1.3:77429535-77429554 MS.gene029220:intron 50.0%
GGAATTTCCAGGAAGCAGGA+AGG - chr1.3:77429968-77429987 MS.gene029220:intron 50.0%
TCTAACTCTCCCAACCGTTC+CGG - chr1.3:77428040-77428059 MS.gene029220:intron 50.0%
TTCCCTTACCACGTTCTCAC+CGG - chr1.3:77428102-77428121 MS.gene029220:intron 50.0%
TTGTACGAACCGGAACGGTT+GGG + chr1.3:77428052-77428071 None:intergenic 50.0%
TTTCCAGGAAGCAGGAAGGA+AGG - chr1.3:77429972-77429991 MS.gene029220:intron 50.0%
! AGAGTCTTTGCCGAGTTGAC+CGG + chr1.3:77428124-77428143 None:intergenic 50.0%
! CAGCATTTTGCGGTTTGCAG+CGG + chr1.3:77428515-77428534 None:intergenic 50.0%
!! CCCCGTTCTTGAAGAGTATC+AGG + chr1.3:77429947-77429966 None:intergenic 50.0%
!! GCTTGCGAGTTGGGTTTTGA+AGG - chr1.3:77429202-77429221 MS.gene029220:intron 50.0%
!! TACAAGTGTCGGTGTCGTGT+CGG + chr1.3:77428972-77428991 None:intergenic 50.0%
!!! GGGTTTTGAAGGGAGGACTT+TGG - chr1.3:77429213-77429232 MS.gene029220:intron 50.0%
!!! TGGGTTTTGGACAAGAGGGA+GGG + chr1.3:77429383-77429402 None:intergenic 50.0%
!!! TTGGGTTTTGGACAAGAGGG+AGG + chr1.3:77429384-77429403 None:intergenic 50.0%
CACGTTCTCACCGGTCAACT+CGG - chr1.3:77428111-77428130 MS.gene029220:intron 55.0%
GACCGGTGAGAACGTGGTAA+GGG + chr1.3:77428107-77428126 None:intergenic 55.0%
GCACGGACACTCCTCGTATT+AGG - chr1.3:77428918-77428937 MS.gene029220:intron 55.0%
GCTGGAGTGATCAAACGGCA+AGG + chr1.3:77428308-77428327 None:intergenic 55.0%
GGGAGGAATTTCCAGGAAGC+AGG - chr1.3:77429964-77429983 MS.gene029220:intron 55.0%
TGACCGGTGAGAACGTGGTA+AGG + chr1.3:77428108-77428127 None:intergenic 55.0%
!! TATTAGGGGTGTCCCGGTGT+CGG - chr1.3:77428934-77428953 MS.gene029220:intron 55.0%
!! TCCTCGTATTAGGGGTGTCC+CGG - chr1.3:77428928-77428947 MS.gene029220:intron 55.0%
!!! GCGAGTTGGGTTTTGAAGGG+AGG - chr1.3:77429206-77429225 MS.gene029220:intron 55.0%
ACCGGGACACCCCTAATACG+AGG + chr1.3:77428932-77428951 None:intergenic 60.0%
ACGGATACGGACATGGACGC+GGG + chr1.3:77429062-77429081 None:intergenic 60.0%
CACGACAAGTGTCCGACACC+GGG + chr1.3:77428949-77428968 None:intergenic 60.0%
CACGGATACGGACATGGACG+CGG + chr1.3:77429063-77429082 None:intergenic 60.0%
GCACCTTCCTTCCTGCTTCC+TGG + chr1.3:77429978-77429997 None:intergenic 60.0%
GCACGACAAGTGTCCGACAC+CGG + chr1.3:77428950-77428969 None:intergenic 60.0%
! CGAGTTGACCGGTGAGAACG+TGG + chr1.3:77428113-77428132 None:intergenic 60.0%
CATGGACGCGGGACACGACA+CGG + chr1.3:77429051-77429070 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 77428005 77430392 77428005 ID=MS.gene029220
chr1.3 mRNA 77428005 77430392 77428005 ID=MS.gene029220.t1;Parent=MS.gene029220
chr1.3 exon 77430051 77430392 77430051 ID=MS.gene029220.t1.exon1;Parent=MS.gene029220.t1
chr1.3 CDS 77430051 77430392 77430051 ID=cds.MS.gene029220.t1;Parent=MS.gene029220.t1
chr1.3 exon 77428390 77428542 77428390 ID=MS.gene029220.t1.exon2;Parent=MS.gene029220.t1
chr1.3 CDS 77428390 77428542 77428390 ID=cds.MS.gene029220.t1;Parent=MS.gene029220.t1
chr1.3 exon 77428005 77428028 77428005 ID=MS.gene029220.t1.exon3;Parent=MS.gene029220.t1
chr1.3 CDS 77428005 77428028 77428005 ID=cds.MS.gene029220.t1;Parent=MS.gene029220.t1
Gene Sequence

>MS.gene029220

ATGGATACGGTGACCTCAACTTCAGCTTCGCTCACTCTAACTCTCCCAACCGTTCCGGTTCGTACAATTACCTCTTCAATCTCCGTATCCTCATCTCTTCCCTTACCACGTTCTCACCGGTCAACTCGGCAAAGACTCTACTCTCTAAAAAGAAGCTTCTCGAATTCTTCTTCTTCTTATGCTGTTCCTAAGTTTACAGTGAGTTGCGGTATCACGGAAATCAATGAAAGCCAGTTCAAGGAAACCGTATTGAAATCTGAGCGTCCGGTTTTAGTTGAATTCGTTGCTACTTGGTGCGGTCCTTGCCGTTTGATCACTCCAGCTATGGAATCCATAGCTCAGGTACCTCTTTCTCTCTTTCTAACTTTTTATTTTGTTTTTGCCGGATACTGAGTTTAAAAAAAGTGTATTTTTTTTCTTAATTGAAACTAACTTTAATTGCATTATGCATGCAATTCACTGTTTAATTGGAACACTTCTGTGCGTAGATTTAAATTGCGGTTGTGTCCGCTGCAAACCGCAAAATGCTGTTGAATTTGCAGTAGTGGATACCTCTAAAATCATTTATATTGGGCTGCGGAACTTAATTGAAACTGAAAGCAATGTTTTTGTGTGCGTAGACTATAACACCTCTTTTTGGGGTGTTGCATAAGACAATAGGTGTTAACAATGTAAATTTTTGTGTGTGGGGGTCTTTTGTTTTGTGTATGTTTCTATCTTCGATTCATGGTACATGTGGATTTCACTCGACAGGAACAATGTTCTTTGTACTTGTTTGTGATAATAGATGTTTTGTGAAAATTATGTGATCTTTCAGGTGCTGCTTTGATTGGTCCTTGTAGTATGATTGTAGACTAAATCTCAATTTTGCAAATCACATTACAACAAAATGTCTTAAGCTTCTGGCTATGAAGCACGGACACTCCTCGTATTAGGGGTGTCCCGGTGTCGGACACTTGTCGTGCCGACACGACACCGACACTTGTAATTAGACTGAATTATGTGATTTTTTCAAATTATTAGTTGTGTCGGCGTGTCAGTGTCCGTGTCGTGTCCCGCGTCCATGTCCGTATCCGTGTTTCATAGGCTTCTGGTGATTGTTGCAAGTGCAATAACCGCGACGTTAGAAGAATGACTTTGAGGAAACAACATTTCTCTTTGTAATCTGTCTTCATTCTGTGATAAAAATTAGCGTTTGCTTGCGAGTTGGGTTTTGAAGGGAGGACTTTGGAGGGTTAAGTTTATATCTTATATTATTTGATATTTTTAAAAAATTGGATGAACATGATAAATGATCATGTAATACTTCAATCACACTTAATTTATTTTTAAAAATGTTTATAGTGTCTTCAATTTTATTAAGAAAAAGGTCTTTCCCTCCCTCTTGTCCAAAACCCAAATTGATGGTCTGATTTAAGGTGTGTTAGCATTCATGATAGGATGCCGGGTCACTGTGTGTTGATTTGAATCTATAATTTCGAGCGACGTAAGAAGTACAGTTAGTACTGATTGAAATGTGAGAAAAGCAATGCTACTGCCATACCAAACAGAGGCTTATTAGTACTGTGCTTTGTTAGTCGTTTATCTTTTTTAGGTTAATGCATTATCATTCATACGAATGCAGGACATGTAAGAGACATTAAGTGAAAAAAAATTAATAGATAGTTATTGAGGCCCTGAGATTCTACCCTTAAAGCCGACATCATTTGCAAATTAAAGTCAATAAAAAATAGAATGCTTTATAATTTGTAGAATGATTGATATGATATTACTAGCCTAGGAGCTATTCTTAAACGTTCTTTGGGATCTTAGTGTTTCTCAACTGAATACTCTCATACATTATATTGCAGGAATATGCAGACAGATTAACAGTTGTAAAGATTGATCATGATGCTAACCCGCAGCTAATTGAAGAGTATAAAGTTTATGGACTTCCAACCCTGATACTCTTCAAGAACGGGGAGGAATTTCCAGGAAGCAGGAAGGAAGGTGCAATTACAAAGGTGAAACTTAAACAACATTTGGATGCTTTATTGGAATCAATATCAGTTACATAGTTATTCGTATTTATGATTCCAAATTGTGACCATTTTTGGGAAACTAGTAATATGGGAGCAGACCTGTTTCATTTAGCATATCAATCAGATTTTATAATTCGCTGAGTATAAAATCTGTATAATGTTGTATTTAGTATTGCACTTAAAAACTTGTGCGAGAAAGAGTGCCTTTGAAGTGTATAATATATACACGTGTACAATTTCAAATTAAAATCGGTCTGATCTGTTTATGGTGATGATGATCTCACTTGTTTTTTGTACTAAGGAATTAGTAATGATATAAATTTATTTGTAAAGAATGACACAGTTTTGTAGCAGGTATATTGCTTAA
Protein sequence

>MS.gene029220.t1

MDTVTSTSASLTLTLPTVPVRTITSSISVSSSLPLPRSHRSTRQRLYSLKRSFSNSSSSYAVPKFTVSCGITEINESQFKETVLKSERPVLVEFVATWCGPCRLITPAMESIAQEYADRLTVVKIDHDANPQLIEEYKVYGLPTLILFKNGEEFPGSRKEGAITKFCSRYIA