AlfalfaGEDB | MS.gene032577

M. sativa cultivar XinJiangDaYe / MS.gene032577

KEGG: K09422
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032577.t1	XP_013450781.1	93.8	209	12	1	1	208	1	209	6.20E-102	380.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032577.t1	P81391	66.4	217	52	4	1	208	1	205	6.0e-69	261.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032577.t1	A0A072U635	93.8	209	12	1	1	208	1	209	4.5e-102	380.2

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene032577.t1	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene028605	MS.gene032577	PPI
MS.gene032577	MS.gene029755	PPI
MS.gene032577	MS.gene30938	PPI
MS.gene032577	MS.gene035013	PPI
MS.gene032577	MS.gene45473	PPI
MS.gene032577	MS.gene051424	PPI
MS.gene032577	MS.gene47440	PPI
MS.gene31174	MS.gene032577	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032577.t1	MTR_6g006030	98.565	209	2	1	1	208	1	209	3.07e-155	428
MS.gene032577.t1	MTR_7g035075	73.148	216	42	5	1	208	1	208	3.28e-106	304
MS.gene032577.t1	MTR_1g110460	73.333	120	32	0	3	122	15	134	1.53e-65	204
MS.gene032577.t1	MTR_1g086510	75.000	108	27	0	14	121	21	128	3.98e-59	186
MS.gene032577.t1	MTR_2g033170	78.947	114	24	0	8	121	18	131	8.09e-58	185
MS.gene032577.t1	MTR_1g086530	72.222	108	30	0	14	121	33	140	1.11e-57	183
MS.gene032577.t1	MTR_4g123040	77.477	111	25	0	11	121	21	131	7.21e-55	177
MS.gene032577.t1	MTR_1g045610	69.524	105	32	0	15	119	22	126	1.05e-51	167
MS.gene032577.t1	MTR_1g073170	66.667	105	35	0	15	119	21	125	2.22e-49	161
MS.gene032577.t1	MTR_4g478180	58.678	121	48	1	1	121	2	120	2.18e-46	156

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032577.t1	AT3G27810	66.981	212	54	7	11	208	17	226	4.45e-90	264
MS.gene032577.t1	AT5G40350	62.069	203	70	2	11	208	14	214	5.07e-84	248
MS.gene032577.t1	AT3G01530	60.287	209	61	6	4	208	12	202	1.86e-77	231
MS.gene032577.t1	AT3G27810	62.500	176	50	7	47	208	4	177	1.18e-63	195
MS.gene032577.t1	AT1G25340	68.293	123	38	1	1	122	4	126	1.14e-61	194
MS.gene032577.t1	AT1G68320	74.107	112	29	0	11	122	16	127	1.20e-61	194
MS.gene032577.t1	AT1G48000	73.832	107	28	0	13	119	31	137	2.67e-59	186
MS.gene032577.t1	AT2G47190	70.536	112	33	0	10	121	16	127	6.74e-58	184
MS.gene032577.t1	AT5G49620	80.357	112	22	0	10	121	22	133	1.03e-56	182
MS.gene032577.t1	AT5G40350	55.090	167	68	2	47	208	5	169	3.09e-56	176

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Find 50 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGCAAAGCATCTTCC+AGG	0.422621	6.3:+1742995	MS.gene032577:CDS
AAAGAAATTACCAGCAGCTT+TGG	0.368872	6.3:-1739047	None:intergenic
AACGTACAGGGAAAAGTTGC+AGG	0.359394	6.3:+1739197	MS.gene032577:CDS
AAGAGGGAATATTACACCTG+AGG	0.685444	6.3:+1739255	MS.gene032577:CDS
AAGATCCTGAAGTGAGAAAA+GGG	0.481473	6.3:+1738948	MS.gene032577:CDS
AAGTCTTCTTCCATGGTCCA+TGG	0.582122	6.3:-1738971	None:intergenic
AATTCCTTAGCCAAAGCTGC+TGG	0.538366	6.3:+1739037	MS.gene032577:CDS
ACAACAATAACAACAATTAT+TGG	0.250956	6.3:+1743278	MS.gene032577:CDS
ACCTTCGTACCAAGGAACTT+TGG	0.437744	6.3:+1743195	MS.gene032577:CDS
ATATTCCCTCTTCTAACATC+TGG	0.433582	6.3:-1739244	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAAGAAATGCAACAAG+TGG	-	chr6.3:1742298-1742317	None:intergenic	30.0%
AATAAAAAATGTTGGAGGGA+TGG	+	chr6.3:1741183-1741202	MS.gene032577:intron	30.0%
AATATATGAACTAGGATAGG+TGG	+	chr6.3:1741296-1741315	MS.gene032577:intron	30.0%
ACAAGTACAAAACACTAAGA+AGG	+	chr6.3:1741665-1741684	MS.gene032577:intron	30.0%
AGAAATATCTTGCGAACATT+AGG	-	chr6.3:1742765-1742784	None:intergenic	30.0%
AGTAAAAGACAAAGACTAAG+AGG	+	chr6.3:1740825-1740844	MS.gene032577:intron	30.0%
ATATAAGAAAGAGAGAGGTT+TGG	+	chr6.3:1741969-1741988	MS.gene032577:intron	30.0%
ATGAGAAATACAACGGAAAA+AGG	-	chr6.3:1739992-1740011	None:intergenic	30.0%
ATTAATTGTGAGAGAGATAG+AGG	-	chr6.3:1739826-1739845	None:intergenic	30.0%
CCTACAAAGAAAAATAAGTG+TGG	+	chr6.3:1741081-1741100	MS.gene032577:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	1738920	1743345	1738920	ID=MS.gene032577
chr6.3	mRNA	1738920	1743345	1738920	ID=MS.gene032577.t1;Parent=MS.gene032577
chr6.3	exon	1738920	1739058	1738920	ID=MS.gene032577.t1.exon1;Parent=MS.gene032577.t1
chr6.3	CDS	1738920	1739058	1738920	ID=cds.MS.gene032577.t1;Parent=MS.gene032577.t1
chr6.3	exon	1739191	1739320	1739191	ID=MS.gene032577.t1.exon2;Parent=MS.gene032577.t1
chr6.3	CDS	1739191	1739320	1739191	ID=cds.MS.gene032577.t1;Parent=MS.gene032577.t1
chr6.3	exon	1742988	1743345	1742988	ID=MS.gene032577.t1.exon3;Parent=MS.gene032577.t1
chr6.3	CDS	1742988	1743345	1742988	ID=cds.MS.gene032577.t1;Parent=MS.gene032577.t1

Gene Sequence

>MS.gene032577

ATGGATAAAAAACCGTGTAACTCATCTCAAGATCCTGAAGTGAGAAAAGGGCCATGGACCATGGAAGAAGACTTGATTTTAATCAATTATATTGCAAATCATGGTGAAGGTGTTTGGAATTCCTTAGCCAAAGCTGCTGGTAATTTCTTTAATAAAAAATCACATTTTTAATTATATATTAATTACATGTTGAGCACTCTTCATATTATTTTCTCAGTAAACCCTAACTAGGGTAATATTTAAATCAACTTTATTTTTTTTTTTCTTCTAGGTCTTAAACGTACAGGGAAAAGTTGCAGGCTTCGATGGCTAAACTACCTTCGTCCAGATGTTAGAAGAGGGAATATTACACCTGAGGAACAACTTTTGATCATGGAACTTCATGCAAAGTGGGGAAATAGGTGAATATTTATATGATTATCATTGTGTTTTATATTTTTATTTTTTTCAAAAAAATTATGTGGTGCATGTGCTCTACCTAACTACTAGATCTTAATTTTTTGTTAAAGAAATGGTATATTTTTAATTATTACTAGCTTAAATTAATTTTTTTCCATTAACCTTTATCTTACAAAGATGAAATAAATTCTTGTTTCTCTATCTCTCCCTCTCCTTATTATATAAGTAAGCACACCATGAGAAAAGTACTATAAGGAAACATTGTGCTTGAGAGAAATCTAGCCAAGAGGAAGACTTCAACACATGGGAGTTTTGTAAAACCTATGGCTGAAAATATTGCTTATACGTGCAAAATCAGTTTCTATGTTTTTAAAGGCTTCAAAATGATTTGTTTTGTGGTTTTATATCCGTATGGGAGTGTTAAGAGAGTTTTCCTTTAAAGGTACATGTTATATGTTTTCCTTTTTGCTTAATTCAATATCTCTCTCCCCTCCTCTCTCTCTCCCTCTATCTCTCTCACAATTAATTCCTTGAGAAATATAAATGAAGATGGAGCTTATAAGTTGTTATGTTGTGGACCTATAAAACATATGCATGGAAGGTTTGGAGATTTTGATCTCACTAGAATACAAGGAATAAAGAGGTGCATCAATGTTATATATTGCCTTCCCCTTTTTCCGTTGTATTTCTCATGCCCTTCTTTTCAAGAAAACAAATCAAGAATTCATATAGTGCAAAGCTTTTCCTTCTTTTCTCTAATTTATTTCTAACTTTTAAAACTTCTAAGAAATTTTTATATATGTTGTGAAAGATGCAGCAAGGTTGGTAGTTAGAGAAACGTTTTAGCTTTACAATTGGAGCTTAGTATACCTTATATAGGTTTCAATCTTTTAAATATATAAATATTTTTTGCATATTAAAAAAATGTATATATGTACTACAGTGATCTTGTACTCTAATTAATAATAGTTTATTTTCTATGAAAAAATAGACTTTAGTTGAATAAGAAAAGTAAAATTCCAAACTCAAGTTAATTTGTTTGTGTATATTTTTTTTTTAAAAATAACAATACAATTTTAATCAACTTGTAGTAAATTGGATCCCTATACGTACCGCCTCTACATCATTTGCAACATTAGGAAAAAAAGAAGAGAAAATGAAATGTAAAATGGAGTGAAACAAGACAAAGAGATAATATGAATCTATGAGAGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATAAAGAGACTGCAAACGGAATGAAGAAATGGAGAGACTAATAAATGGAGAGGATCTTGACTCGGCTAGATGTACATGATCTAGACGTTTACTTTGTAGAAGATGAAAATGTATCATCATGACCTTTAGTGAGAGAGAGAGAGAGAGAGAGAGACTGCAGAGAGAAGCTAGATGTACATGATCTAGATGTTTACGTTGTAAAAAGTGAAAAAGTATACTATCATGACGTATATTGCCTCTCAATAGTAAAAGACAAAGACTAAGAGGAAGATAGATATCTAGAAGATGGAGAGAGCTACCTTTGCATTTATCTTTGCCAAAAAAAAAAGTGAGAGAGAGAACAAGAGGAGATCTTATATAGATGATTGAGATGAAAACAAAAAGAGATAGAGAGAATAAAGGGAGTTTGAGAGAACTCTCACGGTTGAGGACTTGAATCAAATGAAATGATTTCATATAGTAAAAAACAGACTTGGATCCTCTCCAATGTGCATCCCTTTCCTACAAAGAAAAATAAGTGTGGGAGGAGAGGCTAAAAGAAAAAAGAAAAGAAAAAAGGAGAAAAAGATTAAGAGAAAAATGAAATTAAGAGATATAGAGACAATAAAAAATGTTGGAGGGATGGAGAGAAATTTTATTGTAGAGAATACAATTTCGTAAAATTAGAAACACGATAAAAAAAAGTATAAAAAATATTTGATATTCTTAAATTAAAAATATATGAACTAGGATAGGTGGTGTGTGCCTCAATAATAATTATTATAGTATAGTATAGTATATATGATAATGATGCCATTCATTTTCCTATATAATAATGATTGAGAACCAAATAGCTTGGACAAGGTCAGCTTCTATTTTAGGCTGAGCTCATGGAAAGTGGGGGCACATTAAAATAGCACTCTAGTCACATGACAAAGAAATTTCAAGAAGTTTGGTAAAAATAGATCCAAACAAAATGCCATGCAACTTTTGGCTCTTGTGTGAGTGCAATTTTAAATTTTAATGCATACATAAACTATAGCTATAGCTCCACACCCTATCTAGTGTACCTTCCCAATCCTCTAACTCCTTCCCAACCAAGAAAACAAGTACAAAACACTAAGAAGGTAAATAAGTAAATACACAAATAATTTTTTTTTCAACCAAGTTTTGAAATATTATTTATCTTTATTTACATAGTATATATAAAGTTGACAACTTTTATAACTAGCTATGGATAATGAAATCTACTAGGTAATTGGTACACGTGGCATTTTCTTGAACAAAGAGCTAGAAGTTTATTGAAGTTTAAGAGTCATAAAAGAAAAGGAAAAAAAATATGTTTGAAAAAAAATCATTGAAATATTTGAAATTGGAGAGGAACACAACTACTTTATAAAGAGGAATGTATATAAGAAAGAGAGAGGTTTGGAAATGTTTGAATTGAATCAAAATTTCTACTACCTTTGAGACATGTAAGTGAGAAATGTGATATTGGTGATCTTGGAGCCCAAGCTACAAAGCAAAGGCATAAATTTTCCGTTGTATCATCAAAGGGACCTTCAGCATCTTATAACAATAGTCTTTTTATATGGGAAGTCTAACATGCACATCACTTTTATTCTCAACCAAAGAACATGGTCTTACTTGAACACATGACACCTATTTAATATTTTTGTTGCTACTTTTTGCTTATCAATAAAATGTGCACCATTTGACAGCTTTTAATTAATATCCACTTGTTGCATTTCTTTCTTTTTCAAGAAACTTCATATATAATTCCACACATGCACATTTTTCTATAACCTCCTATATCTCCTCATTTTACATATGGTTTTCAAACATACCATGTTCATTTTATAAAAAGGTTTTAAATATACTTAGTCTCATTAATATATAATAATTTATAAAACTAATCATTTGATACAAAGTTTTTAAATCATAATAGTAGCAGTAACAATCAGATACTAGAAAAGAAAAATTAACTACCGACATAAATTAGTGATGAAAAAAATTAAATACCCATTATTCCATCTTCACAAATTTTAGTGACAAAATTAGAGAGTAACTTCAAATAAAAATTCTGTCTAAATTTCTATTACTAATTTACGTTTTTAAGTATTGGATTTATCGTTGTGTCGCGGTCTTTGATATCTGATACTAGAGGCTCTCTAATTTTGACTTAAAAGCGTCCAGTCATAGCCTAATGTTCGCAAGATATTTCTTAAAACACCATGCTTATGATAATAAAAACGCTTTTAAGACAAAATTAAATTTATATGACACTACTAATTATCTAACTGATTGATCATTCGAGAAGCCAAGGTTGATCCAAAGTGAAGGTGTTAATATATATATAGCCAAGCATGAAAATAACTTTTTCAGTTATATGTCACTATCTTTTTATACAAATTATTCACCATTGCAGGTGGTCCAAAATTGCAAAGCATCTTCCAGGAAGAACTGACAATGAGATTAAGAATTTTTGGAGGACTAGGATACAAAAGCACATTAAGCAAGTTGATCATCCTCACCAAAATAATTTCCAACAAATGAGTTTAGAGATAAATGATCATCATCAATCAAGTAGTAGCCAAGTTTCCAACATTGTAGAGCCAATGGAAACTTACTCTTCACCTTCGTACCAAGGAACTTTGGAGCCATTTCCAGCTCAATTTCCAACAATCAATGATCATCCCTCAAGTTGTTGTACAAATGACAACAATAACAACAATTATTGGAGCATGGAGGACATCTGGTCAATGCAATTACTCAATGGAGATTAA

Protein sequence

>MS.gene032577.t1

MDKKPCNSSQDPEVRKGPWTMEEDLILINYIANHGEGVWNSLAKAAGLKRTGKSCRLRWLNYLRPDVRRGNITPEEQLLIMELHAKWGNRWSKIAKHLPGRTDNEIKNFWRTRIQKHIKQVDHPHQNNFQQMSLEINDHHQSSSSQVSNIVEPMETYSSPSYQGTLEPFPAQFPTINDHPSSCCTNDNNNNNYWSMEDIWSMQLLNGD