Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033220.t1 | XP_003608955.1 | 90.9 | 187 | 17 | 0 | 1 | 187 | 1 | 187 | 6.50E-74 | 287 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033220.t1 | G7JNU9 | 90.9 | 187 | 17 | 0 | 1 | 187 | 1 | 187 | 4.7e-74 | 287.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene022274 | MS.gene033220 | PPI |
| MS.gene25931 | MS.gene033220 | PPI |
| MS.gene70603 | MS.gene033220 | PPI |
| MS.gene074378 | MS.gene033220 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033220.t1 | MTR_4g106790 | 96.791 | 187 | 6 | 0 | 1 | 187 | 1 | 187 | 1.36e-134 | 374 |
| MS.gene033220.t1 | MTR_4g106790 | 95.946 | 148 | 6 | 0 | 1 | 148 | 1 | 148 | 4.02e-104 | 296 |
| MS.gene033220.t1 | MTR_3g078300 | 54.737 | 190 | 76 | 3 | 1 | 187 | 1 | 183 | 1.01e-62 | 192 |
| MS.gene033220.t1 | MTR_3g078300 | 54.967 | 151 | 58 | 3 | 1 | 148 | 1 | 144 | 1.43e-47 | 154 |
| MS.gene033220.t1 | MTR_5g035430 | 42.553 | 188 | 87 | 4 | 1 | 185 | 1 | 170 | 5.49e-40 | 134 |
| MS.gene033220.t1 | MTR_4g097220 | 38.830 | 188 | 94 | 4 | 1 | 187 | 1 | 168 | 6.29e-35 | 121 |
| MS.gene033220.t1 | MTR_8g063200 | 37.500 | 184 | 101 | 3 | 1 | 184 | 1 | 170 | 3.38e-31 | 111 |
| MS.gene033220.t1 | MTR_4g097220 | 42.857 | 133 | 57 | 2 | 1 | 133 | 1 | 114 | 2.03e-29 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033220.t1 | AT5G62900 | 47.059 | 187 | 73 | 4 | 1 | 187 | 1 | 161 | 4.18e-48 | 154 |
| MS.gene033220.t1 | AT5G50090 | 44.681 | 188 | 73 | 4 | 1 | 187 | 1 | 158 | 5.50e-42 | 139 |
| MS.gene033220.t1 | AT5G50090 | 46.524 | 187 | 65 | 5 | 1 | 187 | 1 | 152 | 2.65e-41 | 137 |
| MS.gene033220.t1 | AT1G60010 | 41.176 | 187 | 96 | 4 | 1 | 187 | 1 | 173 | 8.51e-41 | 136 |
| MS.gene033220.t1 | AT5G50090 | 53.782 | 119 | 42 | 1 | 1 | 119 | 1 | 106 | 1.02e-35 | 122 |
| MS.gene033220.t1 | AT1G10530 | 36.508 | 189 | 95 | 4 | 1 | 187 | 1 | 166 | 7.75e-34 | 118 |
| MS.gene033220.t1 | AT5G67620 | 32.353 | 204 | 98 | 7 | 1 | 187 | 1 | 181 | 7.60e-24 | 93.2 |
Find 25 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTTCTTCTTTGCCATT+TGG | 0.302263 | 8.3:+16845088 | None:intergenic |
| ACAAAAGTCAAATCAGATTA+AGG | 0.303462 | 8.3:-16844642 | MS.gene033220:CDS |
| AGATTTACCTTGTTATCTTC+AGG | 0.363890 | 8.3:+16844584 | None:intergenic |
| TTAATGATAAAACAGCTAAT+AGG | 0.376595 | 8.3:-16844613 | MS.gene033220:CDS |
| ATCACTTCACTAGCAGTAAC+TGG | 0.406024 | 8.3:+16845058 | None:intergenic |
| AGAAGAGCAACATAGTGATC+AGG | 0.427746 | 8.3:+16845025 | None:intergenic |
| CAGAGGTTACAAAGGGTATG+TGG | 0.456771 | 8.3:-16844712 | MS.gene033220:CDS |
| TTCAGTGATGCTTTGTAAAG+AGG | 0.477664 | 8.3:+16844024 | None:intergenic |
| GCAAAGAAGAAAGGTTGTAT+TGG | 0.478560 | 8.3:-16845081 | MS.gene033220:CDS |
| AGGACAAAAGCAACAACAAA+TGG | 0.492369 | 8.3:-16844082 | MS.gene033220:CDS |
| TCAGTGATGCTTTGTAAAGA+GGG | 0.514695 | 8.3:+16844025 | None:intergenic |
| AACCAAATGGCAAAGAAGAA+AGG | 0.522282 | 8.3:-16845090 | MS.gene033220:CDS |
| TTGGTTTCAGAGGTTACAAA+GGG | 0.536135 | 8.3:-16844719 | MS.gene033220:intron |
| GATTCAAAACTCATATCAGC+TGG | 0.538326 | 8.3:+16843997 | None:intergenic |
| CTTGTGATACAGCAACCAAA+TGG | 0.541988 | 8.3:-16845103 | MS.gene033220:CDS |
| GAAGAGCAACATAGTGATCA+GGG | 0.575247 | 8.3:+16845026 | None:intergenic |
| CCAAGCAAAAGTGTATCTGT+TGG | 0.576593 | 8.3:+16844884 | None:intergenic |
| TTTGGTTTCAGAGGTTACAA+AGG | 0.584060 | 8.3:-16844720 | MS.gene033220:intron |
| AGAGGTTACAAAGGGTATGT+GGG | 0.585748 | 8.3:-16844711 | MS.gene033220:CDS |
| TATAGACTCATCTCAGCTCA+AGG | 0.593800 | 8.3:-16844854 | MS.gene033220:intron |
| GTCTGAACCTGAAGATAACA+AGG | 0.608807 | 8.3:-16844591 | MS.gene033220:intron |
| AGTCAAATCAGATTAAGGAA+AGG | 0.621653 | 8.3:-16844637 | MS.gene033220:CDS |
| AATCTGAAAGACATGAGTCA+AGG | 0.637514 | 8.3:-16844102 | MS.gene033220:CDS |
| ACAAAAGCAACAACAAATGG+TGG | 0.646145 | 8.3:-16844079 | MS.gene033220:CDS |
| ATAATAATAAGTCAAGAACA+TGG | 0.691937 | 8.3:-16844051 | MS.gene033220:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTAATAATAATTTATGAT+TGG | - | chr8.3:16845002-16845021 | MS.gene033220:CDS | 10.0% |
| !! | TTATTTACATTAACTAATTA+AGG | - | chr8.3:16844855-16844874 | MS.gene033220:CDS | 10.0% |
| !! | CAATGCATAATATATATATA+AGG | + | chr8.3:16844795-16844814 | None:intergenic | 15.0% |
| !! | TAATATATATATAAGGTTTG+AGG | + | chr8.3:16844788-16844807 | None:intergenic | 15.0% |
| !! | TATAAAGAATCAAAAGAAAA+AGG | + | chr8.3:16844350-16844369 | None:intergenic | 15.0% |
| !!! | AAGAACAAAATAATCTTTTA+AGG | + | chr8.3:16844384-16844403 | None:intergenic | 15.0% |
| !!! | AGAACAAAATAATCTTTTAA+GGG | + | chr8.3:16844383-16844402 | None:intergenic | 15.0% |
| !! | ATAATAATAAGTCAAGAACA+TGG | - | chr8.3:16845097-16845116 | MS.gene033220:CDS | 20.0% |
| !! | TTAATGATAAAACAGCTAAT+AGG | - | chr8.3:16844535-16844554 | MS.gene033220:intron | 20.0% |
| ! | ACAAAAGTCAAATCAGATTA+AGG | - | chr8.3:16844506-16844525 | MS.gene033220:intron | 25.0% |
| ! | TACATTAACTAATTAAGGTC+TGG | - | chr8.3:16844860-16844879 | MS.gene033220:CDS | 25.0% |
| ! | TATAGTTACTGTTCATAAGA+AGG | + | chr8.3:16844711-16844730 | None:intergenic | 25.0% |
| !! | GGTCTGGTTATAAATTTTAT+TGG | - | chr8.3:16844876-16844895 | MS.gene033220:CDS | 25.0% |
| !!! | ATGATTGGTTCATTATTTTC+AGG | - | chr8.3:16845017-16845036 | MS.gene033220:CDS | 25.0% |
| !!! | TTGAGTCTTTATGTTGTTTT+TGG | - | chr8.3:16844410-16844429 | MS.gene033220:intron | 25.0% |
| AAGAACATGAATATCTTCAG+TGG | - | chr8.3:16844644-16844663 | MS.gene033220:CDS | 30.0% | |
| AGATTCAATCAACATGGATT+TGG | + | chr8.3:16844622-16844641 | None:intergenic | 30.0% | |
| AGTCAAATCAGATTAAGGAA+AGG | - | chr8.3:16844511-16844530 | MS.gene033220:intron | 30.0% | |
| ! | AGATTTACCTTGTTATCTTC+AGG | + | chr8.3:16844567-16844586 | None:intergenic | 30.0% |
| !! | TAAGAGAGAGATTTGTTGAA+TGG | - | chr8.3:16844978-16844997 | MS.gene033220:CDS | 30.0% |
| !!! | TATGTTGTTTTTGGTTTCAG+AGG | - | chr8.3:16844419-16844438 | MS.gene033220:intron | 30.0% |
| !!! | TTTTGAAGTGCATAATGTTG+TGG | + | chr8.3:16844154-16844173 | None:intergenic | 30.0% |
| AACCAAATGGCAAAGAAGAA+AGG | - | chr8.3:16844058-16844077 | MS.gene033220:CDS | 35.0% | |
| AACCTTTCTTCTTTGCCATT+TGG | + | chr8.3:16844063-16844082 | None:intergenic | 35.0% | |
| AATCTGAAAGACATGAGTCA+AGG | - | chr8.3:16845046-16845065 | MS.gene033220:CDS | 35.0% | |
| ACAAAAGCAACAACAAATGG+TGG | - | chr8.3:16845069-16845088 | MS.gene033220:CDS | 35.0% | |
| AGGACAAAAGCAACAACAAA+TGG | - | chr8.3:16845066-16845085 | MS.gene033220:CDS | 35.0% | |
| GCAAAGAAGAAAGGTTGTAT+TGG | - | chr8.3:16844067-16844086 | MS.gene033220:CDS | 35.0% | |
| TACTGTTCATAAGAAGGAGT+TGG | + | chr8.3:16844705-16844724 | None:intergenic | 35.0% | |
| TCAGTGATGCTTTGTAAAGA+GGG | + | chr8.3:16845126-16845145 | None:intergenic | 35.0% | |
| TCTTGCAGATTCAATCAACA+TGG | + | chr8.3:16844628-16844647 | None:intergenic | 35.0% | |
| TTCAGTGATGCTTTGTAAAG+AGG | + | chr8.3:16845127-16845146 | None:intergenic | 35.0% | |
| TTGGTTTCAGAGGTTACAAA+GGG | - | chr8.3:16844429-16844448 | MS.gene033220:intron | 35.0% | |
| ! | TTTGGTTTCAGAGGTTACAA+AGG | - | chr8.3:16844428-16844447 | MS.gene033220:intron | 35.0% |
| !!! | TTTGACTTTTGTGCTGATTC+TGG | + | chr8.3:16844498-16844517 | None:intergenic | 35.0% |
| AGAAGAGCAACATAGTGATC+AGG | + | chr8.3:16844126-16844145 | None:intergenic | 40.0% | |
| ATCACTTCACTAGCAGTAAC+TGG | + | chr8.3:16844093-16844112 | None:intergenic | 40.0% | |
| CCTTGCTACTACAAAGTCAA+TGG | + | chr8.3:16844928-16844947 | None:intergenic | 40.0% | |
| CTTGTGATACAGCAACCAAA+TGG | - | chr8.3:16844045-16844064 | MS.gene033220:CDS | 40.0% | |
| GAAGAGCAACATAGTGATCA+GGG | + | chr8.3:16844125-16844144 | None:intergenic | 40.0% | |
| GTCTGAACCTGAAGATAACA+AGG | - | chr8.3:16844557-16844576 | MS.gene033220:intron | 40.0% | |
| TATAGACTCATCTCAGCTCA+AGG | - | chr8.3:16844294-16844313 | MS.gene033220:intron | 40.0% | |
| ! | AGAGGTTACAAAGGGTATGT+GGG | - | chr8.3:16844437-16844456 | MS.gene033220:intron | 40.0% |
| ! | CCAACAGATACACTTTTGCT+TGG | - | chr8.3:16844264-16844283 | MS.gene033220:intron | 40.0% |
| ! | CCAAGCAAAAGTGTATCTGT+TGG | + | chr8.3:16844267-16844286 | None:intergenic | 40.0% |
| !! | CCATTGACTTTGTAGTAGCA+AGG | - | chr8.3:16844925-16844944 | MS.gene033220:CDS | 40.0% |
| ! | CAGAGGTTACAAAGGGTATG+TGG | - | chr8.3:16844436-16844455 | MS.gene033220:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 16844012 | 16845158 | 16844012 | ID=MS.gene033220 |
| chr8.3 | mRNA | 16844012 | 16845158 | 16844012 | ID=MS.gene033220.t1;Parent=MS.gene033220 |
| chr8.3 | exon | 16844855 | 16845158 | 16844855 | ID=MS.gene033220.t1.exon1;Parent=MS.gene033220.t1 |
| chr8.3 | CDS | 16844855 | 16845158 | 16844855 | ID=cds.MS.gene033220.t1;Parent=MS.gene033220.t1 |
| chr8.3 | exon | 16844592 | 16844731 | 16844592 | ID=MS.gene033220.t1.exon2;Parent=MS.gene033220.t1 |
| chr8.3 | CDS | 16844592 | 16844731 | 16844592 | ID=cds.MS.gene033220.t1;Parent=MS.gene033220.t1 |
| chr8.3 | exon | 16844012 | 16844131 | 16844012 | ID=MS.gene033220.t1.exon3;Parent=MS.gene033220.t1 |
| chr8.3 | CDS | 16844012 | 16844131 | 16844012 | ID=cds.MS.gene033220.t1;Parent=MS.gene033220.t1 |
| Gene Sequence |
| Protein sequence |
