Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70603.t1 | XP_013461027.1 | 93 | 187 | 9 | 1 | 1 | 187 | 1 | 183 | 2.10E-88 | 335.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70603.t1 | A0A072V025 | 93.0 | 187 | 9 | 1 | 1 | 187 | 1 | 183 | 1.5e-88 | 335.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70603 | MS.gene43289 | PPI |
| MS.gene70603 | MS.gene033220 | PPI |
| MS.gene70603 | MS.gene60343 | PPI |
| MS.gene70603 | MS.gene56948 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70603.t1 | MTR_3g078300 | 93.048 | 187 | 9 | 1 | 1 | 187 | 1 | 183 | 2.65e-128 | 358 |
| MS.gene70603.t1 | MTR_3g078300 | 91.946 | 149 | 8 | 1 | 1 | 149 | 1 | 145 | 3.98e-98 | 282 |
| MS.gene70603.t1 | MTR_4g106790 | 54.974 | 191 | 78 | 3 | 1 | 187 | 1 | 187 | 1.70e-58 | 181 |
| MS.gene70603.t1 | MTR_4g106790 | 54.902 | 153 | 61 | 3 | 1 | 149 | 1 | 149 | 4.55e-43 | 142 |
| MS.gene70603.t1 | MTR_5g035430 | 40.761 | 184 | 94 | 2 | 1 | 184 | 1 | 169 | 6.46e-40 | 134 |
| MS.gene70603.t1 | MTR_8g063200 | 41.146 | 192 | 89 | 5 | 1 | 187 | 1 | 173 | 1.23e-35 | 123 |
| MS.gene70603.t1 | MTR_4g097220 | 34.021 | 194 | 95 | 4 | 1 | 187 | 1 | 168 | 9.74e-29 | 105 |
| MS.gene70603.t1 | MTR_4g097220 | 42.241 | 116 | 48 | 2 | 1 | 116 | 1 | 97 | 2.95e-23 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70603.t1 | AT1G10530 | 43.850 | 187 | 84 | 6 | 1 | 187 | 1 | 166 | 6.27e-43 | 141 |
| MS.gene70603.t1 | AT5G50090 | 46.524 | 187 | 65 | 4 | 1 | 187 | 1 | 152 | 3.17e-42 | 139 |
| MS.gene70603.t1 | AT1G60010 | 41.711 | 187 | 95 | 5 | 1 | 187 | 1 | 173 | 6.49e-42 | 139 |
| MS.gene70603.t1 | AT5G50090 | 45.789 | 190 | 68 | 5 | 1 | 187 | 1 | 158 | 1.60e-41 | 137 |
| MS.gene70603.t1 | AT5G62900 | 43.316 | 187 | 80 | 4 | 1 | 187 | 1 | 161 | 1.87e-39 | 132 |
| MS.gene70603.t1 | AT5G50090 | 55.085 | 118 | 39 | 1 | 1 | 118 | 1 | 104 | 8.21e-36 | 122 |
| MS.gene70603.t1 | AT5G67620 | 38.235 | 136 | 57 | 4 | 1 | 135 | 1 | 110 | 2.09e-23 | 92.0 |
Find 37 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCCTTGCTTGAGATCTTTC+TGG | 0.152334 | 3.1:-64247454 | None:intergenic |
| CCTAATGATGCTCTTCTTCT+TGG | 0.200037 | 3.1:+64246828 | MS.gene70603:CDS |
| AAAGTTGTCTTCACCAAATC+TGG | 0.293990 | 3.1:-64247034 | None:intergenic |
| CAACTTTCAATGTCCTTGTT+TGG | 0.305139 | 3.1:-64246726 | None:intergenic |
| GGGGTTAGAAATGGAGAAAA+AGG | 0.355393 | 3.1:+64247057 | MS.gene70603:CDS |
| GATTTGGTGAAGACAACTTT+GGG | 0.355948 | 3.1:+64247037 | MS.gene70603:CDS |
| TGCTTGAGATCTTTCTGGTT+TGG | 0.357296 | 3.1:-64247449 | None:intergenic |
| CATGTCTCATAAACCAGATT+TGG | 0.362054 | 3.1:+64247021 | MS.gene70603:CDS |
| GTTGTTGTTGATGTTGGTTT+TGG | 0.367219 | 3.1:-64246772 | None:intergenic |
| GACAACTTTGGGGTTAGAAA+TGG | 0.376259 | 3.1:+64247048 | MS.gene70603:CDS |
| CAGAGGTTATAAAGGGCATA+AGG | 0.401834 | 3.1:+64246969 | MS.gene70603:intron |
| GTTTGACATACATGACAATA+AGG | 0.416543 | 3.1:+64247087 | MS.gene70603:CDS |
| TTGCATCTCTGTCTCTCAGC+TGG | 0.425317 | 3.1:-64247557 | None:intergenic |
| TTGTGTTCAGAGGTTATAAA+GGG | 0.425799 | 3.1:+64246962 | MS.gene70603:intron |
| GTTGTGTTCAGAGGTTATAA+AGG | 0.434348 | 3.1:+64246961 | MS.gene70603:intron |
| CCAAGAAGAAGAGCATCATT+AGG | 0.444033 | 3.1:-64246828 | None:intergenic |
| TCACAGCTAAGACAAGATTC+TGG | 0.458380 | 3.1:+64247503 | MS.gene70603:CDS |
| AGATTTGGTGAAGACAACTT+TGG | 0.460928 | 3.1:+64247036 | MS.gene70603:CDS |
| AACTTGGTTGTTGTTGATGT+TGG | 0.469472 | 3.1:-64246778 | None:intergenic |
| GAGAAGAGCAACACAATGAC+CGG | 0.504515 | 3.1:-64246685 | None:intergenic |
| TTATACGAGTTAATCGAACT+TGG | 0.518883 | 3.1:-64246794 | None:intergenic |
| TCTGAGATACTCTGTAAAGA+TGG | 0.532885 | 3.1:-64247529 | None:intergenic |
| GGCAGCATTATTAGTTGATG+TGG | 0.546831 | 3.1:-64247476 | None:intergenic |
| AGAGGTTATAAAGGGCATAA+GGG | 0.552926 | 3.1:+64246970 | MS.gene70603:intron |
| TCTTGTCTTAGCTGTGACAA+TGG | 0.579137 | 3.1:-64247497 | None:intergenic |
| TATAGACTCATCACAACTCA+AGG | 0.595309 | 3.1:+64246858 | MS.gene70603:CDS |
| AGAAGAGCAACACAATGACC+GGG | 0.604281 | 3.1:-64246684 | None:intergenic |
| CACTACAGTATATCCAAACA+AGG | 0.611228 | 3.1:+64246713 | MS.gene70603:CDS |
| CTTGTTTGGATATACTGTAG+TGG | 0.620700 | 3.1:-64246712 | None:intergenic |
| AACCAGAAAGATCTCAAGCA+AGG | 0.637849 | 3.1:+64247452 | MS.gene70603:CDS |
| TAGTGATACAACACCCAAGT+GGG | 0.648394 | 3.1:+64246607 | MS.gene70603:CDS |
| ATTTGGTGAAGACAACTTTG+GGG | 0.688333 | 3.1:+64247038 | MS.gene70603:CDS |
| AAATTGTCAAGCTATTGATG+CGG | 0.702309 | 3.1:+64246578 | MS.gene70603:CDS |
| CTAGTGATACAACACCCAAG+TGG | 0.711840 | 3.1:+64246606 | MS.gene70603:CDS |
| TTGTGATGAGTCTATAAACA+TGG | 0.715588 | 3.1:-64246851 | None:intergenic |
| CAAGTTATGAAAATGAACCC+CGG | 0.718116 | 3.1:+64246666 | MS.gene70603:CDS |
| GAAGAGCAACACAATGACCG+GGG | 0.757492 | 3.1:-64246683 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAAAAACAAATAAAAAAA+TGG | - | chr3.1:64247127-64247146 | None:intergenic | 10.0% |
| !! | CCTCAAAATTCTTTATATTA+CGG | + | chr3.1:64247331-64247350 | MS.gene70603:intron | 20.0% |
| !!! | TGTAATTGTTATGATTTTTC+AGG | + | chr3.1:64247423-64247442 | MS.gene70603:intron | 20.0% |
| !!! | TTATTTCGTTCTTTTAAATG+TGG | + | chr3.1:64247268-64247287 | MS.gene70603:intron | 20.0% |
| ! | AACACAACAAACATTTCAAA+AGG | - | chr3.1:64246949-64246968 | None:intergenic | 25.0% |
| ! | AGTTATAGCAAAATCAAGTT+TGG | + | chr3.1:64247152-64247171 | MS.gene70603:intron | 25.0% |
| ! | AGTTGCTCCAAAAATAATAA+TGG | + | chr3.1:64246744-64246763 | MS.gene70603:CDS | 25.0% |
| ! | CAATTTGTGTAATTCTCTAT+TGG | + | chr3.1:64246884-64246903 | MS.gene70603:intron | 25.0% |
| !! | CCGTAATATAAAGAATTTTG+AGG | - | chr3.1:64247334-64247353 | None:intergenic | 25.0% |
| !!! | AAACAGTGGATTTTATCATT+GGG | + | chr3.1:64247195-64247214 | MS.gene70603:intron | 25.0% |
| AAATTGTCAAGCTATTGATG+CGG | + | chr3.1:64246578-64246597 | MS.gene70603:CDS | 30.0% | |
| CAAAAGGTCAATCTCAAATT+TGG | - | chr3.1:64246933-64246952 | None:intergenic | 30.0% | |
| GTTTGACATACATGACAATA+AGG | + | chr3.1:64247087-64247106 | MS.gene70603:CDS | 30.0% | |
| TTATACGAGTTAATCGAACT+TGG | - | chr3.1:64246797-64246816 | None:intergenic | 30.0% | |
| TTGTGATGAGTCTATAAACA+TGG | - | chr3.1:64246854-64246873 | None:intergenic | 30.0% | |
| TTGTGTTCAGAGGTTATAAA+GGG | + | chr3.1:64246962-64246981 | MS.gene70603:intron | 30.0% | |
| ! | CATAGCATGGTTTAAAATTG+CGG | - | chr3.1:64247376-64247395 | None:intergenic | 30.0% |
| !! | AACATCATTTTTGCAAACAG+TGG | + | chr3.1:64247181-64247200 | MS.gene70603:intron | 30.0% |
| !! | CAAACAGTGGATTTTATCAT+TGG | + | chr3.1:64247194-64247213 | MS.gene70603:intron | 30.0% |
| !! | GGTGTCACCATTATTATTTT+TGG | - | chr3.1:64246754-64246773 | None:intergenic | 30.0% |
| AAAGTTGTCTTCACCAAATC+TGG | - | chr3.1:64247037-64247056 | None:intergenic | 35.0% | |
| AAATGGAGAAAAAGGCAAAG+AGG | + | chr3.1:64247065-64247084 | MS.gene70603:CDS | 35.0% | |
| AGATCTCTTCAAACATAGCA+TGG | - | chr3.1:64247389-64247408 | None:intergenic | 35.0% | |
| CAACTTTCAATGTCCTTGTT+TGG | - | chr3.1:64246729-64246748 | None:intergenic | 35.0% | |
| CAAGTTATGAAAATGAACCC+CGG | + | chr3.1:64246666-64246685 | MS.gene70603:CDS | 35.0% | |
| CACTACAGTATATCCAAACA+AGG | + | chr3.1:64246713-64246732 | MS.gene70603:CDS | 35.0% | |
| CATGTCTCATAAACCAGATT+TGG | + | chr3.1:64247021-64247040 | MS.gene70603:CDS | 35.0% | |
| CTTGTTTGGATATACTGTAG+TGG | - | chr3.1:64246715-64246734 | None:intergenic | 35.0% | |
| GTTGTGTTCAGAGGTTATAA+AGG | + | chr3.1:64246961-64246980 | MS.gene70603:intron | 35.0% | |
| TATAGACTCATCACAACTCA+AGG | + | chr3.1:64246858-64246877 | MS.gene70603:CDS | 35.0% | |
| ! | AACTTGGTTGTTGTTGATGT+TGG | - | chr3.1:64246781-64246800 | None:intergenic | 35.0% |
| ! | AATTTCTCTGTTTTCCCACT+TGG | - | chr3.1:64246624-64246643 | None:intergenic | 35.0% |
| ! | AGAGGTTATAAAGGGCATAA+GGG | + | chr3.1:64246970-64246989 | MS.gene70603:intron | 35.0% |
| ! | ATTTCTCTGTTTTCCCACTT+GGG | - | chr3.1:64246623-64246642 | None:intergenic | 35.0% |
| ! | ATTTGGTGAAGACAACTTTG+GGG | + | chr3.1:64247038-64247057 | MS.gene70603:CDS | 35.0% |
| ! | TCTGAGATACTCTGTAAAGA+TGG | - | chr3.1:64247532-64247551 | None:intergenic | 35.0% |
| !! | AGATTTGGTGAAGACAACTT+TGG | + | chr3.1:64247036-64247055 | MS.gene70603:CDS | 35.0% |
| !! | GAAATGTTTGTTGTGTTCAG+AGG | + | chr3.1:64246952-64246971 | MS.gene70603:intron | 35.0% |
| !! | GATTTGGTGAAGACAACTTT+GGG | + | chr3.1:64247037-64247056 | MS.gene70603:CDS | 35.0% |
| !!! | GTTGTTGTTGATGTTGGTTT+TGG | - | chr3.1:64246775-64246794 | None:intergenic | 35.0% |
| AACCAGAAAGATCTCAAGCA+AGG | + | chr3.1:64247452-64247471 | MS.gene70603:CDS | 40.0% | |
| CCTAATGATGCTCTTCTTCT+TGG | + | chr3.1:64246828-64246847 | MS.gene70603:CDS | 40.0% | |
| GGGGTTAGAAATGGAGAAAA+AGG | + | chr3.1:64247057-64247076 | MS.gene70603:CDS | 40.0% | |
| TAGTGATACAACACCCAAGT+GGG | + | chr3.1:64246607-64246626 | MS.gene70603:CDS | 40.0% | |
| TCACAGCTAAGACAAGATTC+TGG | + | chr3.1:64247503-64247522 | MS.gene70603:CDS | 40.0% | |
| TCTTGTCTTAGCTGTGACAA+TGG | - | chr3.1:64247500-64247519 | None:intergenic | 40.0% | |
| ! | ATGGTTTAAAATTGCGGTCC+GGG | - | chr3.1:64247370-64247389 | None:intergenic | 40.0% |
| ! | CAGAGGTTATAAAGGGCATA+AGG | + | chr3.1:64246969-64246988 | MS.gene70603:intron | 40.0% |
| ! | CATGGTTTAAAATTGCGGTC+CGG | - | chr3.1:64247371-64247390 | None:intergenic | 40.0% |
| ! | CGGGGTTCATTTTCATAACT+TGG | - | chr3.1:64246668-64246687 | None:intergenic | 40.0% |
| ! | GGCAGCATTATTAGTTGATG+TGG | - | chr3.1:64247479-64247498 | None:intergenic | 40.0% |
| !! | CCAAGAAGAAGAGCATCATT+AGG | - | chr3.1:64246831-64246850 | None:intergenic | 40.0% |
| !! | GACAACTTTGGGGTTAGAAA+TGG | + | chr3.1:64247048-64247067 | MS.gene70603:CDS | 40.0% |
| !! | TGCTTGAGATCTTTCTGGTT+TGG | - | chr3.1:64247452-64247471 | None:intergenic | 40.0% |
| AGAAGAGCAACACAATGACC+GGG | - | chr3.1:64246687-64246706 | None:intergenic | 45.0% | |
| AGCCACAATTTCGACTGCAT+CGG | - | chr3.1:64247294-64247313 | None:intergenic | 45.0% | |
| CTAGTGATACAACACCCAAG+TGG | + | chr3.1:64246606-64246625 | MS.gene70603:CDS | 45.0% | |
| GAGAAGAGCAACACAATGAC+CGG | - | chr3.1:64246688-64246707 | None:intergenic | 45.0% | |
| GTCCTTGCTTGAGATCTTTC+TGG | - | chr3.1:64247457-64247476 | None:intergenic | 45.0% | |
| GAAGAGCAACACAATGACCG+GGG | - | chr3.1:64246686-64246705 | None:intergenic | 50.0% | |
| TACGGCTACAATTGCAGTCC+CGG | + | chr3.1:64247349-64247368 | MS.gene70603:intron | 50.0% | |
| GGCCGATGCAGTCGAAATTG+TGG | + | chr3.1:64247289-64247308 | MS.gene70603:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 64246573 | 64247564 | 64246573 | ID=MS.gene70603 |
| chr3.1 | mRNA | 64246573 | 64247564 | 64246573 | ID=MS.gene70603.t1;Parent=MS.gene70603 |
| chr3.1 | exon | 64246573 | 64246879 | 64246573 | ID=MS.gene70603.t1.exon1;Parent=MS.gene70603.t1 |
| chr3.1 | CDS | 64246573 | 64246879 | 64246573 | ID=cds.MS.gene70603.t1;Parent=MS.gene70603.t1 |
| chr3.1 | exon | 64246972 | 64247108 | 64246972 | ID=MS.gene70603.t1.exon2;Parent=MS.gene70603.t1 |
| chr3.1 | CDS | 64246972 | 64247108 | 64246972 | ID=cds.MS.gene70603.t1;Parent=MS.gene70603.t1 |
| chr3.1 | exon | 64247445 | 64247564 | 64247445 | ID=MS.gene70603.t1.exon3;Parent=MS.gene70603.t1 |
| chr3.1 | CDS | 64247445 | 64247564 | 64247445 | ID=cds.MS.gene70603.t1;Parent=MS.gene70603.t1 |
| Gene Sequence |
| Protein sequence |
