Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25931.t1 | XP_013461027.1 | 92.2 | 153 | 7 | 2 | 1 | 153 | 36 | 183 | 2.20E-67 | 265 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25931.t1 | A0A072V025 | 92.2 | 153 | 7 | 2 | 1 | 153 | 36 | 183 | 1.6e-67 | 265.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43289 | MS.gene25931 | PPI |
| MS.gene60343 | MS.gene25931 | PPI |
| MS.gene25931 | MS.gene033220 | PPI |
| MS.gene25931 | MS.gene56948 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25931.t1 | MTR_3g078300 | 92.157 | 153 | 7 | 2 | 1 | 153 | 36 | 183 | 7.71e-100 | 285 |
| MS.gene25931.t1 | MTR_3g078300 | 74.830 | 147 | 27 | 3 | 1 | 142 | 36 | 177 | 2.67e-71 | 212 |
| MS.gene25931.t1 | MTR_4g106790 | 50.000 | 156 | 69 | 3 | 2 | 153 | 37 | 187 | 6.00e-37 | 125 |
| MS.gene25931.t1 | MTR_4g106790 | 49.153 | 118 | 51 | 3 | 2 | 115 | 37 | 149 | 5.78e-23 | 89.4 |
| MS.gene25931.t1 | MTR_5g035430 | 37.333 | 150 | 76 | 3 | 2 | 150 | 37 | 169 | 8.76e-21 | 83.6 |
| MS.gene25931.t1 | MTR_8g063200 | 35.897 | 156 | 75 | 7 | 2 | 152 | 37 | 172 | 9.74e-16 | 70.5 |
| MS.gene25931.t1 | MTR_4g097220 | 30.189 | 159 | 77 | 4 | 2 | 153 | 37 | 168 | 8.64e-15 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25931.t1 | AT5G50090 | 42.949 | 156 | 53 | 6 | 1 | 153 | 36 | 158 | 5.33e-25 | 94.4 |
| MS.gene25931.t1 | AT1G10530 | 41.935 | 155 | 64 | 8 | 1 | 153 | 36 | 166 | 9.91e-25 | 94.0 |
| MS.gene25931.t1 | AT5G50090 | 44.444 | 153 | 49 | 5 | 1 | 153 | 36 | 152 | 1.26e-24 | 93.2 |
| MS.gene25931.t1 | AT5G62900 | 40.789 | 152 | 63 | 5 | 2 | 153 | 37 | 161 | 3.15e-23 | 89.7 |
| MS.gene25931.t1 | AT1G60010 | 40.127 | 157 | 71 | 7 | 1 | 153 | 36 | 173 | 1.09e-21 | 86.3 |
| MS.gene25931.t1 | AT5G50090 | 53.571 | 84 | 24 | 2 | 1 | 84 | 36 | 104 | 9.20e-19 | 77.8 |
Find 34 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAATGATGCTCTTCTTCT+TGG | 0.200037 | 3.2:+69192942 | MS.gene25931:CDS |
| GTCCTTGCTTGAGATCTTTC+CGG | 0.253662 | 3.2:-69193575 | None:intergenic |
| CAATTCTGAATTTCCTTGTT+TGG | 0.279815 | 3.2:-69192837 | None:intergenic |
| AAAGTTGTCTTCACCAAATC+TGG | 0.293990 | 3.2:-69193148 | None:intergenic |
| GTTGTTGATGGTGTTGGTTT+TGG | 0.353662 | 3.2:-69192886 | None:intergenic |
| GATTTGGTGAAGACAACTTT+GGG | 0.355948 | 3.2:+69193151 | MS.gene25931:CDS |
| CATGTCTCATAAACCAGATT+TGG | 0.362054 | 3.2:+69193135 | MS.gene25931:CDS |
| GACAACTTTGGGGTTAGAAA+TGG | 0.376259 | 3.2:+69193162 | MS.gene25931:CDS |
| GTTTGACACACAGGACAATA+AGG | 0.400976 | 3.2:+69193201 | MS.gene25931:CDS |
| TGCTTGAGATCTTTCCGGTT+TGG | 0.406315 | 3.2:-69193570 | None:intergenic |
| TAATCGAACTTGGTTGTTGA+TGG | 0.408166 | 3.2:-69192898 | None:intergenic |
| CAGAGGTTATGAAGGGCATA+AGG | 0.419227 | 3.2:+69193083 | MS.gene25931:intron |
| TTGCATCTCTGTCTCTCAGC+TGG | 0.425317 | 3.2:-69193678 | None:intergenic |
| CCAAGAAGAAGAGCATCATT+AGG | 0.444033 | 3.2:-69192942 | None:intergenic |
| TCACAGCTAAGACAAGATTC+TGG | 0.458380 | 3.2:+69193624 | MS.gene25931:CDS |
| AGATTTGGTGAAGACAACTT+TGG | 0.460928 | 3.2:+69193150 | MS.gene25931:CDS |
| AACTTGGTTGTTGATGGTGT+TGG | 0.504816 | 3.2:-69192892 | None:intergenic |
| AGCAAAGAGGTTTGACACAC+AGG | 0.505844 | 3.2:+69193192 | MS.gene25931:CDS |
| GTTGTGTTCAGAGGTTATGA+AGG | 0.516516 | 3.2:+69193075 | MS.gene25931:intron |
| TTATACGAGTTAATCGAACT+TGG | 0.518883 | 3.2:-69192908 | None:intergenic |
| TCTGAGATACTCTGTAAAGA+TGG | 0.532885 | 3.2:-69193650 | None:intergenic |
| CTTGTTTGGATATACTGTAG+TGG | 0.551280 | 3.2:-69192823 | None:intergenic |
| AGAGGTTATGAAGGGCATAA+GGG | 0.564067 | 3.2:+69193084 | MS.gene25931:intron |
| GGCAGCACTATTAGTTGATG+TGG | 0.566673 | 3.2:-69193597 | None:intergenic |
| TTGTGTTCAGAGGTTATGAA+GGG | 0.572211 | 3.2:+69193076 | MS.gene25931:intron |
| TCTTGTCTTAGCTGTGACAA+TGG | 0.579137 | 3.2:-69193618 | None:intergenic |
| CACTACAGTATATCCAAACA+AGG | 0.586755 | 3.2:+69192824 | MS.gene25931:CDS |
| TATAGACTCATCACAACTCA+AGG | 0.595309 | 3.2:+69192972 | MS.gene25931:CDS |
| AGAAGAGCAACACAATGACC+AGG | 0.606509 | 3.2:-69192795 | None:intergenic |
| AACCGGAAAGATCTCAAGCA+AGG | 0.640145 | 3.2:+69193573 | MS.gene25931:CDS |
| CAAGTTATGAAAATGAACCC+TGG | 0.679229 | 3.2:+69192777 | None:intergenic |
| ATTTGGTGAAGACAACTTTG+GGG | 0.688333 | 3.2:+69193152 | MS.gene25931:CDS |
| TTGTGATGAGTCTATAAACA+TGG | 0.715588 | 3.2:-69192965 | None:intergenic |
| GAAGAGCAACACAATGACCA+GGG | 0.717756 | 3.2:-69192794 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAACAAATAAAAAAAA+TGG | - | chr3.2:69193241-69193260 | None:intergenic | 10.0% |
| !!! | AACACAACAAATATTTTAAA+GGG | - | chr3.2:69193063-69193082 | None:intergenic | 15.0% |
| !! | AATTGCTCCAAAAATAATAA+TGG | + | chr3.2:69192855-69192874 | MS.gene25931:CDS | 20.0% |
| !! | TTCACTAATTATATACATGA+AGG | + | chr3.2:69193373-69193392 | MS.gene25931:intron | 20.0% |
| !!! | AAACAATGGATTTTATCATT+GGG | + | chr3.2:69193308-69193327 | MS.gene25931:intron | 20.0% |
| !!! | CATAGATATAAAGAATTTTG+AGG | - | chr3.2:69193498-69193517 | None:intergenic | 20.0% |
| !!! | GAACACAACAAATATTTTAA+AGG | - | chr3.2:69193064-69193083 | None:intergenic | 20.0% |
| !!! | TTTTCCTTTTCTTTTAAATG+TGG | + | chr3.2:69193432-69193451 | MS.gene25931:intron | 20.0% |
| !! | CAATTTGTGTAATTCTCTTT+TGG | + | chr3.2:69192998-69193017 | MS.gene25931:intron | 25.0% |
| !!! | AAAATATTTGTTGTGTTCAG+AGG | + | chr3.2:69193066-69193085 | MS.gene25931:intron | 25.0% |
| !!! | AACATCATTTTTGCAAACAA+TGG | + | chr3.2:69193294-69193313 | MS.gene25931:intron | 25.0% |
| !!! | CAAACAATGGATTTTATCAT+TGG | + | chr3.2:69193307-69193326 | MS.gene25931:intron | 25.0% |
| !!! | TTGTAAAGTTCTGATTTTTC+AGG | + | chr3.2:69193544-69193563 | MS.gene25931:intron | 25.0% |
| AAATGGAGAAAAAAGCAAAG+AGG | + | chr3.2:69193179-69193198 | MS.gene25931:CDS | 30.0% | |
| AAGGTTTGACTAGATTGTTT+AGG | + | chr3.2:69193392-69193411 | MS.gene25931:intron | 30.0% | |
| CAATTCTGAATTTCCTTGTT+TGG | - | chr3.2:69192840-69192859 | None:intergenic | 30.0% | |
| TAAAGGGTCAATCTCAAATT+TGG | - | chr3.2:69193047-69193066 | None:intergenic | 30.0% | |
| TCGACCACATTTAAAAGAAA+AGG | - | chr3.2:69193439-69193458 | None:intergenic | 30.0% | |
| TGCTCCAAAAATAATAATGG+TGG | + | chr3.2:69192858-69192877 | MS.gene25931:CDS | 30.0% | |
| TTATACGAGTTAATCGAACT+TGG | - | chr3.2:69192911-69192930 | None:intergenic | 30.0% | |
| TTGTGATGAGTCTATAAACA+TGG | - | chr3.2:69192968-69192987 | None:intergenic | 30.0% | |
| ! | GTCACCACCATTATTATTTT+TGG | - | chr3.2:69192865-69192884 | None:intergenic | 30.0% |
| AAAGTTGTCTTCACCAAATC+TGG | - | chr3.2:69193151-69193170 | None:intergenic | 35.0% | |
| CACTACAGTATATCCAAACA+AGG | + | chr3.2:69192824-69192843 | MS.gene25931:CDS | 35.0% | |
| CATGTCTCATAAACCAGATT+TGG | + | chr3.2:69193135-69193154 | MS.gene25931:CDS | 35.0% | |
| CTTGTTTGGATATACTGTAG+TGG | - | chr3.2:69192826-69192845 | None:intergenic | 35.0% | |
| TAATCGAACTTGGTTGTTGA+TGG | - | chr3.2:69192901-69192920 | None:intergenic | 35.0% | |
| TATAGACTCATCACAACTCA+AGG | + | chr3.2:69192972-69192991 | MS.gene25931:CDS | 35.0% | |
| TTGTGTTCAGAGGTTATGAA+GGG | + | chr3.2:69193076-69193095 | MS.gene25931:intron | 35.0% | |
| ! | ATTTGGTGAAGACAACTTTG+GGG | + | chr3.2:69193152-69193171 | MS.gene25931:CDS | 35.0% |
| ! | TCTGAGATACTCTGTAAAGA+TGG | - | chr3.2:69193653-69193672 | None:intergenic | 35.0% |
| !! | AGATTTGGTGAAGACAACTT+TGG | + | chr3.2:69193150-69193169 | MS.gene25931:CDS | 35.0% |
| !! | GATTTGGTGAAGACAACTTT+GGG | + | chr3.2:69193151-69193170 | MS.gene25931:CDS | 35.0% |
| CCTAATGATGCTCTTCTTCT+TGG | + | chr3.2:69192942-69192961 | MS.gene25931:CDS | 40.0% | |
| GTTGTGTTCAGAGGTTATGA+AGG | + | chr3.2:69193075-69193094 | MS.gene25931:intron | 40.0% | |
| GTTTGACACACAGGACAATA+AGG | + | chr3.2:69193201-69193220 | MS.gene25931:CDS | 40.0% | |
| TCACAGCTAAGACAAGATTC+TGG | + | chr3.2:69193624-69193643 | MS.gene25931:CDS | 40.0% | |
| TCTTGTCTTAGCTGTGACAA+TGG | - | chr3.2:69193621-69193640 | None:intergenic | 40.0% | |
| ! | AGAGGTTATGAAGGGCATAA+GGG | + | chr3.2:69193084-69193103 | MS.gene25931:intron | 40.0% |
| !! | AACTTGGTTGTTGATGGTGT+TGG | - | chr3.2:69192895-69192914 | None:intergenic | 40.0% |
| !! | CCAAGAAGAAGAGCATCATT+AGG | - | chr3.2:69192945-69192964 | None:intergenic | 40.0% |
| !! | GACAACTTTGGGGTTAGAAA+TGG | + | chr3.2:69193162-69193181 | MS.gene25931:CDS | 40.0% |
| !!! | GATTTTTCAGGCTACCAAAC+CGG | + | chr3.2:69193556-69193575 | MS.gene25931:intron | 40.0% |
| !!! | GTTGTTGATGGTGTTGGTTT+TGG | - | chr3.2:69192889-69192908 | None:intergenic | 40.0% |
| AACCGGAAAGATCTCAAGCA+AGG | + | chr3.2:69193573-69193592 | MS.gene25931:CDS | 45.0% | |
| AGAAGAGCAACACAATGACC+AGG | - | chr3.2:69192798-69192817 | None:intergenic | 45.0% | |
| AGCAAAGAGGTTTGACACAC+AGG | + | chr3.2:69193192-69193211 | MS.gene25931:CDS | 45.0% | |
| GAAGAGCAACACAATGACCA+GGG | - | chr3.2:69192797-69192816 | None:intergenic | 45.0% | |
| GTCCTTGCTTGAGATCTTTC+CGG | - | chr3.2:69193578-69193597 | None:intergenic | 45.0% | |
| TGCTTGAGATCTTTCCGGTT+TGG | - | chr3.2:69193573-69193592 | None:intergenic | 45.0% | |
| ! | CAGAGGTTATGAAGGGCATA+AGG | + | chr3.2:69193083-69193102 | MS.gene25931:intron | 45.0% |
| ! | GGCAGCACTATTAGTTGATG+TGG | - | chr3.2:69193600-69193619 | None:intergenic | 45.0% |
| GGTCGATGCAGTCGAAATTG+TGG | + | chr3.2:69193453-69193472 | MS.gene25931:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 69192789 | 69193685 | 69192789 | ID=MS.gene25931 |
| chr3.2 | mRNA | 69192789 | 69193685 | 69192789 | ID=MS.gene25931.t1;Parent=MS.gene25931 |
| chr3.2 | exon | 69192789 | 69192993 | 69192789 | ID=MS.gene25931.t1.exon1;Parent=MS.gene25931.t1 |
| chr3.2 | CDS | 69192789 | 69192993 | 69192789 | ID=cds.MS.gene25931.t1;Parent=MS.gene25931.t1 |
| chr3.2 | exon | 69193086 | 69193222 | 69193086 | ID=MS.gene25931.t1.exon2;Parent=MS.gene25931.t1 |
| chr3.2 | CDS | 69193086 | 69193222 | 69193086 | ID=cds.MS.gene25931.t1;Parent=MS.gene25931.t1 |
| chr3.2 | exon | 69193566 | 69193685 | 69193566 | ID=MS.gene25931.t1.exon3;Parent=MS.gene25931.t1 |
| chr3.2 | CDS | 69193566 | 69193685 | 69193566 | ID=cds.MS.gene25931.t1;Parent=MS.gene25931.t1 |
| Gene Sequence |
| Protein sequence |
