Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022274.t1 | XP_013461027.1 | 93.1 | 188 | 8 | 2 | 1 | 188 | 1 | 183 | 1.00E-87 | 332.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022274.t1 | A0A072V025 | 93.1 | 188 | 8 | 2 | 1 | 188 | 1 | 183 | 7.5e-88 | 332.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43289 | MS.gene022274 | PPI |
| MS.gene60343 | MS.gene022274 | PPI |
| MS.gene022274 | MS.gene033220 | PPI |
| MS.gene022274 | MS.gene56948 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022274.t1 | MTR_3g078300 | 93.085 | 188 | 8 | 2 | 1 | 188 | 1 | 183 | 1.06e-127 | 357 |
| MS.gene022274.t1 | MTR_3g078300 | 93.333 | 150 | 5 | 2 | 1 | 150 | 1 | 145 | 6.71e-99 | 284 |
| MS.gene022274.t1 | MTR_4g106790 | 53.646 | 192 | 80 | 3 | 1 | 188 | 1 | 187 | 1.08e-57 | 179 |
| MS.gene022274.t1 | MTR_4g106790 | 53.896 | 154 | 62 | 3 | 1 | 150 | 1 | 149 | 3.47e-43 | 142 |
| MS.gene022274.t1 | MTR_5g035430 | 41.081 | 185 | 93 | 2 | 1 | 185 | 1 | 169 | 3.03e-40 | 134 |
| MS.gene022274.t1 | MTR_8g063200 | 41.667 | 192 | 87 | 7 | 1 | 187 | 1 | 172 | 1.35e-35 | 123 |
| MS.gene022274.t1 | MTR_4g097220 | 33.333 | 195 | 96 | 4 | 1 | 188 | 1 | 168 | 2.98e-28 | 103 |
| MS.gene022274.t1 | MTR_4g097220 | 41.026 | 117 | 49 | 2 | 1 | 117 | 1 | 97 | 1.10e-22 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022274.t1 | AT1G10530 | 43.617 | 188 | 84 | 6 | 1 | 188 | 1 | 166 | 1.58e-43 | 143 |
| MS.gene022274.t1 | AT5G50090 | 46.597 | 191 | 66 | 6 | 1 | 188 | 1 | 158 | 2.99e-43 | 142 |
| MS.gene022274.t1 | AT5G50090 | 46.316 | 190 | 62 | 6 | 1 | 188 | 1 | 152 | 1.89e-42 | 140 |
| MS.gene022274.t1 | AT5G62900 | 45.213 | 188 | 76 | 5 | 1 | 188 | 1 | 161 | 6.11e-42 | 139 |
| MS.gene022274.t1 | AT1G60010 | 41.667 | 192 | 89 | 6 | 1 | 188 | 1 | 173 | 6.57e-41 | 136 |
| MS.gene022274.t1 | AT5G50090 | 56.303 | 119 | 37 | 2 | 1 | 119 | 1 | 104 | 1.54e-36 | 124 |
| MS.gene022274.t1 | AT5G67620 | 31.250 | 208 | 96 | 6 | 1 | 188 | 1 | 181 | 2.29e-24 | 94.4 |
Find 35 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAATGATGCTCTTCTTCT+TGG | 0.200037 | 3.3:+66378099 | MS.gene022274:CDS |
| GTCCTTGCTTGAGATCTTTC+CGG | 0.253662 | 3.3:-66378732 | None:intergenic |
| CAATTCTGAATTTCCTTGTT+TGG | 0.279815 | 3.3:-66377994 | None:intergenic |
| AAAGTTGTCTTCACCAAATC+TGG | 0.293990 | 3.3:-66378305 | None:intergenic |
| GTTGTTGATGGTGTTGGTTT+TGG | 0.353662 | 3.3:-66378043 | None:intergenic |
| GATTTGGTGAAGACAACTTT+GGG | 0.355948 | 3.3:+66378308 | MS.gene022274:CDS |
| CATGTCTCATAAACCAGATT+TGG | 0.362054 | 3.3:+66378292 | MS.gene022274:CDS |
| GACAACTTTGGGGTTAGAAA+TGG | 0.376259 | 3.3:+66378319 | MS.gene022274:CDS |
| GTTTGACACACAGGACAATA+AGG | 0.400976 | 3.3:+66378358 | MS.gene022274:CDS |
| TAATCGAACTTGGTTGTTGA+TGG | 0.408166 | 3.3:-66378055 | None:intergenic |
| CAGAGGTTATGAAGGGCATA+AGG | 0.419227 | 3.3:+66378240 | MS.gene022274:intron |
| TTGCTTCTCTGTCTCTCAGC+TGG | 0.441329 | 3.3:-66378835 | None:intergenic |
| CCAAGAAGAAGAGCATCATT+AGG | 0.444033 | 3.3:-66378099 | None:intergenic |
| TCACAGCTAAGACAAGATTC+TGG | 0.458380 | 3.3:+66378781 | MS.gene022274:CDS |
| AGATTTGGTGAAGACAACTT+TGG | 0.460928 | 3.3:+66378307 | MS.gene022274:CDS |
| AACTTGGTTGTTGATGGTGT+TGG | 0.504816 | 3.3:-66378049 | None:intergenic |
| AGCAAAGAGGTTTGACACAC+AGG | 0.505844 | 3.3:+66378349 | MS.gene022274:CDS |
| GTTGTGTTCAGAGGTTATGA+AGG | 0.516516 | 3.3:+66378232 | MS.gene022274:intron |
| TTATACGAGTTAATCGAACT+TGG | 0.518883 | 3.3:-66378065 | None:intergenic |
| TCTGAGATACTCTGTAAAGA+TGG | 0.532885 | 3.3:-66378807 | None:intergenic |
| GGCAGCATTATTAGTTGATG+TGG | 0.546831 | 3.3:-66378754 | None:intergenic |
| CTTGTTTGGATATACTGTAG+TGG | 0.551280 | 3.3:-66377980 | None:intergenic |
| AGAGGTTATGAAGGGCATAA+GGG | 0.564067 | 3.3:+66378241 | MS.gene022274:intron |
| TTGTGTTCAGAGGTTATGAA+GGG | 0.572211 | 3.3:+66378233 | MS.gene022274:intron |
| TCTTGTCTTAGCTGTGACAA+TGG | 0.579137 | 3.3:-66378775 | None:intergenic |
| CACTACAGTATATCCAAACA+AGG | 0.586755 | 3.3:+66377981 | MS.gene022274:CDS |
| TATAGACTCATCACAACTCA+AGG | 0.595309 | 3.3:+66378129 | MS.gene022274:CDS |
| AGAAGAGCAACACAATGACC+AGG | 0.606509 | 3.3:-66377952 | None:intergenic |
| AACCGGAAAGATCTCAAGCA+AGG | 0.640145 | 3.3:+66378730 | MS.gene022274:CDS |
| TAGTGATACAACACCCAAGT+GGG | 0.648394 | 3.3:+66377875 | MS.gene022274:CDS |
| CAAGTTATGAAAATGAACCC+TGG | 0.679229 | 3.3:+66377934 | MS.gene022274:CDS |
| ATTTGGTGAAGACAACTTTG+GGG | 0.688333 | 3.3:+66378309 | MS.gene022274:CDS |
| CTAGTGATACAACACCCAAG+TGG | 0.711840 | 3.3:+66377874 | MS.gene022274:CDS |
| TTGTGATGAGTCTATAAACA+TGG | 0.715588 | 3.3:-66378122 | None:intergenic |
| GAAGAGCAACACAATGACCA+GGG | 0.717756 | 3.3:-66377951 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAACAAATAAAAAAAA+TGG | - | chr3.3:66378398-66378417 | None:intergenic | 10.0% |
| !!! | AACACAACAAATATTTTAAA+GGG | - | chr3.3:66378220-66378239 | None:intergenic | 15.0% |
| !!! | TTTTCCTTTTATTTTAAATG+TGG | + | chr3.3:66378589-66378608 | MS.gene022274:intron | 15.0% |
| !! | AATTGCTCCAAAAATAATAA+TGG | + | chr3.3:66378012-66378031 | MS.gene022274:CDS | 20.0% |
| !! | TTCACTATTTATATACATGA+AGG | + | chr3.3:66378530-66378549 | MS.gene022274:intron | 20.0% |
| !!! | AAACAATGGATTTTATCATT+GGG | + | chr3.3:66378465-66378484 | MS.gene022274:intron | 20.0% |
| !!! | CATAGATATAAAGAATTTTG+AGG | - | chr3.3:66378655-66378674 | None:intergenic | 20.0% |
| !!! | GAACACAACAAATATTTTAA+AGG | - | chr3.3:66378221-66378240 | None:intergenic | 20.0% |
| !! | CAATTTGTGTAATTCTCTTT+TGG | + | chr3.3:66378155-66378174 | MS.gene022274:intron | 25.0% |
| !! | TCGACCACATTTAAAATAAA+AGG | - | chr3.3:66378596-66378615 | None:intergenic | 25.0% |
| !!! | AAAATATTTGTTGTGTTCAG+AGG | + | chr3.3:66378223-66378242 | MS.gene022274:intron | 25.0% |
| !!! | AACATCATTTTTGCAAACAA+TGG | + | chr3.3:66378451-66378470 | MS.gene022274:intron | 25.0% |
| !!! | CAAACAATGGATTTTATCAT+TGG | + | chr3.3:66378464-66378483 | MS.gene022274:intron | 25.0% |
| !!! | TTGTAAAGTTCTGATTTTTC+AGG | + | chr3.3:66378701-66378720 | MS.gene022274:intron | 25.0% |
| AAATGGAGAAAAAAGCAAAG+AGG | + | chr3.3:66378336-66378355 | MS.gene022274:CDS | 30.0% | |
| AAGGTTTGACTAGATTGTTT+AGG | + | chr3.3:66378549-66378568 | MS.gene022274:intron | 30.0% | |
| CAATTCTGAATTTCCTTGTT+TGG | - | chr3.3:66377997-66378016 | None:intergenic | 30.0% | |
| TAAAGGGTCAATCTCAAATT+TGG | - | chr3.3:66378204-66378223 | None:intergenic | 30.0% | |
| TGCTCCAAAAATAATAATGG+TGG | + | chr3.3:66378015-66378034 | MS.gene022274:CDS | 30.0% | |
| TTATACGAGTTAATCGAACT+TGG | - | chr3.3:66378068-66378087 | None:intergenic | 30.0% | |
| TTGTGATGAGTCTATAAACA+TGG | - | chr3.3:66378125-66378144 | None:intergenic | 30.0% | |
| ! | GTCACCACCATTATTATTTT+TGG | - | chr3.3:66378022-66378041 | None:intergenic | 30.0% |
| AAAGTTGTCTTCACCAAATC+TGG | - | chr3.3:66378308-66378327 | None:intergenic | 35.0% | |
| CAAGTTATGAAAATGAACCC+TGG | + | chr3.3:66377934-66377953 | MS.gene022274:CDS | 35.0% | |
| CACTACAGTATATCCAAACA+AGG | + | chr3.3:66377981-66378000 | MS.gene022274:CDS | 35.0% | |
| CATGTCTCATAAACCAGATT+TGG | + | chr3.3:66378292-66378311 | MS.gene022274:CDS | 35.0% | |
| CTTGTTTGGATATACTGTAG+TGG | - | chr3.3:66377983-66378002 | None:intergenic | 35.0% | |
| TAATCGAACTTGGTTGTTGA+TGG | - | chr3.3:66378058-66378077 | None:intergenic | 35.0% | |
| TATAGACTCATCACAACTCA+AGG | + | chr3.3:66378129-66378148 | MS.gene022274:CDS | 35.0% | |
| TTGTGTTCAGAGGTTATGAA+GGG | + | chr3.3:66378233-66378252 | MS.gene022274:intron | 35.0% | |
| ! | AATTTCTCTGTTTTCCCACT+TGG | - | chr3.3:66377892-66377911 | None:intergenic | 35.0% |
| ! | ATTTCTCTGTTTTCCCACTT+GGG | - | chr3.3:66377891-66377910 | None:intergenic | 35.0% |
| ! | ATTTGGTGAAGACAACTTTG+GGG | + | chr3.3:66378309-66378328 | MS.gene022274:CDS | 35.0% |
| ! | CAGGGTTCATTTTCATAACT+TGG | - | chr3.3:66377936-66377955 | None:intergenic | 35.0% |
| ! | TCTGAGATACTCTGTAAAGA+TGG | - | chr3.3:66378810-66378829 | None:intergenic | 35.0% |
| !! | AGATTTGGTGAAGACAACTT+TGG | + | chr3.3:66378307-66378326 | MS.gene022274:CDS | 35.0% |
| !! | GATTTGGTGAAGACAACTTT+GGG | + | chr3.3:66378308-66378327 | MS.gene022274:CDS | 35.0% |
| !!! | GATTTTTCAGGTTACGAAAC+CGG | + | chr3.3:66378713-66378732 | MS.gene022274:intron | 35.0% |
| CCTAATGATGCTCTTCTTCT+TGG | + | chr3.3:66378099-66378118 | MS.gene022274:CDS | 40.0% | |
| GTTGTGTTCAGAGGTTATGA+AGG | + | chr3.3:66378232-66378251 | MS.gene022274:intron | 40.0% | |
| GTTTGACACACAGGACAATA+AGG | + | chr3.3:66378358-66378377 | MS.gene022274:CDS | 40.0% | |
| TAGTGATACAACACCCAAGT+GGG | + | chr3.3:66377875-66377894 | MS.gene022274:CDS | 40.0% | |
| TCACAGCTAAGACAAGATTC+TGG | + | chr3.3:66378781-66378800 | MS.gene022274:CDS | 40.0% | |
| TCTTGTCTTAGCTGTGACAA+TGG | - | chr3.3:66378778-66378797 | None:intergenic | 40.0% | |
| ! | AGAGGTTATGAAGGGCATAA+GGG | + | chr3.3:66378241-66378260 | MS.gene022274:intron | 40.0% |
| ! | GGCAGCATTATTAGTTGATG+TGG | - | chr3.3:66378757-66378776 | None:intergenic | 40.0% |
| !! | AACTTGGTTGTTGATGGTGT+TGG | - | chr3.3:66378052-66378071 | None:intergenic | 40.0% |
| !! | CCAAGAAGAAGAGCATCATT+AGG | - | chr3.3:66378102-66378121 | None:intergenic | 40.0% |
| !! | GACAACTTTGGGGTTAGAAA+TGG | + | chr3.3:66378319-66378338 | MS.gene022274:CDS | 40.0% |
| !!! | GTTGTTGATGGTGTTGGTTT+TGG | - | chr3.3:66378046-66378065 | None:intergenic | 40.0% |
| AACCGGAAAGATCTCAAGCA+AGG | + | chr3.3:66378730-66378749 | MS.gene022274:CDS | 45.0% | |
| AGAAGAGCAACACAATGACC+AGG | - | chr3.3:66377955-66377974 | None:intergenic | 45.0% | |
| AGCAAAGAGGTTTGACACAC+AGG | + | chr3.3:66378349-66378368 | MS.gene022274:CDS | 45.0% | |
| CTAGTGATACAACACCCAAG+TGG | + | chr3.3:66377874-66377893 | MS.gene022274:CDS | 45.0% | |
| GAAGAGCAACACAATGACCA+GGG | - | chr3.3:66377954-66377973 | None:intergenic | 45.0% | |
| GTCCTTGCTTGAGATCTTTC+CGG | - | chr3.3:66378735-66378754 | None:intergenic | 45.0% | |
| ! | CAGAGGTTATGAAGGGCATA+AGG | + | chr3.3:66378240-66378259 | MS.gene022274:intron | 45.0% |
| GGTCGATGCAGTCGAAATTG+TGG | + | chr3.3:66378610-66378629 | MS.gene022274:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 66377841 | 66378842 | 66377841 | ID=MS.gene022274 |
| chr3.3 | mRNA | 66377841 | 66378842 | 66377841 | ID=MS.gene022274.t1;Parent=MS.gene022274 |
| chr3.3 | exon | 66377841 | 66378150 | 66377841 | ID=MS.gene022274.t1.exon1;Parent=MS.gene022274.t1 |
| chr3.3 | CDS | 66377841 | 66378150 | 66377841 | ID=cds.MS.gene022274.t1;Parent=MS.gene022274.t1 |
| chr3.3 | exon | 66378243 | 66378379 | 66378243 | ID=MS.gene022274.t1.exon2;Parent=MS.gene022274.t1 |
| chr3.3 | CDS | 66378243 | 66378379 | 66378243 | ID=cds.MS.gene022274.t1;Parent=MS.gene022274.t1 |
| chr3.3 | exon | 66378723 | 66378842 | 66378723 | ID=MS.gene022274.t1.exon3;Parent=MS.gene022274.t1 |
| chr3.3 | CDS | 66378723 | 66378842 | 66378723 | ID=cds.MS.gene022274.t1;Parent=MS.gene022274.t1 |
| Gene Sequence |
| Protein sequence |
