AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033400.t1 MTR_4g102520 97.956 587 8 2 1 583 1 587 0.0 1199
MS.gene033400.t1 MTR_1g072740 96.629 534 15 2 1 531 1 534 0.0 1075
MS.gene033400.t1 MTR_8g467040 33.226 310 193 4 278 577 107 412 1.63e-51 183
MS.gene033400.t1 MTR_4g119620 27.660 329 211 10 266 580 6 321 7.06e-33 129
MS.gene033400.t1 MTR_1g072140 25.243 309 218 8 277 581 14 313 8.37e-30 120
MS.gene033400.t1 MTR_7g086360 26.730 318 220 9 264 577 32 340 3.01e-26 110
MS.gene033400.t1 MTR_8g092620 27.687 307 203 10 278 580 6 297 9.74e-25 105
MS.gene033400.t1 MTR_8g092620 25.000 260 142 9 279 492 47 299 1.44e-12 69.3
MS.gene033400.t1 MTR_4g104380 25.962 312 206 8 278 580 218 513 1.33e-24 108
MS.gene033400.t1 MTR_3g075400 26.471 306 214 8 277 580 212 508 1.09e-23 105
MS.gene033400.t1 MTR_8g092620 27.541 305 202 10 278 578 13 302 3.07e-23 101
MS.gene033400.t1 MTR_2g436510 26.299 308 195 12 287 580 167 456 3.23e-19 91.3
MS.gene033400.t1 MTR_7g076230 26.132 287 188 9 288 556 367 647 5.33e-19 91.7
MS.gene033400.t1 MTR_8g092620 28.774 212 140 7 278 487 6 208 1.27e-16 79.7
MS.gene033400.t1 MTR_6g004040 29.825 171 111 6 281 449 92 255 1.40e-16 84.0
MS.gene033400.t1 MTR_6g004040 23.264 288 197 10 286 561 12 287 1.02e-14 78.2
MS.gene033400.t1 MTR_6g004040 24.868 189 135 4 392 580 31 212 4.02e-13 72.8
MS.gene033400.t1 MTR_6g004040 31.538 130 80 4 280 406 133 256 2.09e-11 67.4
MS.gene033400.t1 MTR_1g069345 25.000 320 223 11 267 580 195 503 4.16e-16 81.6
MS.gene033400.t1 MTR_2g012630 29.545 176 114 7 281 454 91 258 1.50e-15 80.9
MS.gene033400.t1 MTR_2g012630 23.737 198 144 4 383 580 21 211 5.54e-13 72.8
MS.gene033400.t1 MTR_2g012630 22.867 293 198 10 283 561 8 286 1.22e-12 71.6
MS.gene033400.t1 MTR_2g012630 29.545 176 114 7 281 454 91 258 1.72e-15 80.9
MS.gene033400.t1 MTR_2g012630 23.737 198 144 4 383 580 21 211 6.88e-13 72.4
MS.gene033400.t1 MTR_2g012630 22.867 293 198 10 283 561 8 286 1.47e-12 71.2
MS.gene033400.t1 MTR_1g054300 23.037 382 204 10 281 581 110 482 1.73e-15 79.7
MS.gene033400.t1 MTR_1g097140 25.329 304 204 14 283 580 73 359 2.41e-15 79.0
MS.gene033400.t1 MTR_5g090420 21.338 314 234 8 272 580 243 548 8.81e-15 77.8
MS.gene033400.t1 MTR_3g052220 23.824 319 217 11 279 580 202 511 1.68e-14 76.6
MS.gene033400.t1 MTR_3g116500 23.975 317 217 10 285 582 60 371 1.39e-13 73.2
MS.gene033400.t1 MTR_3g116500 25.991 227 152 7 369 582 38 261 7.23e-13 69.7
MS.gene033400.t1 MTR_8g106790 23.022 278 183 12 278 535 54 320 9.74e-13 70.1
MS.gene033400.t1 MTR_4g123597 23.125 320 211 14 281 580 347 651 2.46e-12 70.5
MS.gene033400.t1 MTR_4g105700 21.498 307 214 11 294 582 316 613 6.74e-12 68.9
MS.gene033400.t1 MTR_2g060520 21.429 322 228 8 279 580 115 431 8.99e-12 67.8
MS.gene033400.t1 MTR_1g034785 24.339 189 117 5 281 447 109 293 6.45e-11 65.1
MS.gene033400.t1 MTR_8g104010 22.333 300 193 8 281 580 581 840 9.52e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033400.t1 AT1G10580 77.797 581 119 4 1 580 1 572 0.0 936
MS.gene033400.t1 AT5G54520 32.915 319 204 3 270 580 140 456 1.33e-54 192
MS.gene033400.t1 AT3G49660 25.641 312 214 9 277 580 11 312 5.20e-30 120
MS.gene033400.t1 AT3G49660 28.767 219 145 6 282 495 105 317 1.51e-25 108
MS.gene033400.t1 AT5G64730 27.508 309 201 11 278 580 6 297 6.81e-27 111
MS.gene033400.t1 AT4G15900 29.967 307 183 12 288 580 174 462 3.53e-26 112
MS.gene033400.t1 AT2G43770 26.460 291 206 7 287 574 50 335 9.07e-26 109
MS.gene033400.t1 AT4G02730 24.595 309 215 10 279 580 32 329 4.20e-24 103
MS.gene033400.t1 AT3G16650 28.990 307 185 13 288 580 168 455 5.31e-22 100
MS.gene033400.t1 AT5G50230 24.658 292 196 7 278 560 213 489 4.63e-20 94.4
MS.gene033400.t1 AT5G23430 23.226 310 206 13 283 582 9 296 4.21e-18 89.0
MS.gene033400.t1 AT5G23430 30.000 150 97 5 281 428 92 235 1.47e-14 77.8
MS.gene033400.t1 AT5G23430 29.921 127 86 3 280 406 133 256 3.24e-11 67.0
MS.gene033400.t1 AT5G23430 23.226 310 206 13 283 582 9 296 4.55e-18 89.0
MS.gene033400.t1 AT5G23430 30.000 150 97 5 281 428 92 235 1.54e-14 77.8
MS.gene033400.t1 AT5G23430 29.921 127 86 3 280 406 133 256 3.32e-11 67.0
MS.gene033400.t1 AT5G23430 23.226 310 206 13 283 582 9 296 4.55e-18 89.0
MS.gene033400.t1 AT5G23430 30.000 150 97 5 281 428 92 235 1.54e-14 77.8
MS.gene033400.t1 AT5G23430 29.921 127 86 3 280 406 133 256 3.32e-11 67.0
MS.gene033400.t1 AT5G08390 23.453 307 209 12 283 582 9 296 9.31e-18 87.8
MS.gene033400.t1 AT5G08390 32.000 150 94 5 281 428 92 235 2.01e-15 80.5
MS.gene033400.t1 AT5G08390 32.283 127 83 3 280 406 133 256 1.92e-12 70.9
MS.gene033400.t1 AT5G08560 23.453 307 222 8 278 580 262 559 1.19e-17 87.0
MS.gene033400.t1 AT5G08560 23.453 307 222 8 278 580 262 559 1.19e-17 87.0
MS.gene033400.t1 AT5G08560 23.453 307 222 8 278 580 262 559 1.19e-17 87.0
MS.gene033400.t1 AT1G61210 31.429 175 112 6 281 454 91 258 6.81e-17 85.5
MS.gene033400.t1 AT1G61210 25.000 304 196 12 288 581 13 294 5.48e-16 82.4
MS.gene033400.t1 AT1G61210 31.008 129 82 5 280 406 132 255 1.40e-11 68.2
MS.gene033400.t1 AT1G61210 31.429 175 112 6 281 454 91 258 8.64e-17 85.1
MS.gene033400.t1 AT1G61210 25.000 304 196 12 288 581 13 294 8.04e-16 82.0
MS.gene033400.t1 AT1G61210 31.008 129 82 5 280 406 132 255 1.69e-11 68.2
MS.gene033400.t1 AT1G61210 31.429 175 112 6 281 454 91 258 9.84e-17 84.7
MS.gene033400.t1 AT1G61210 25.000 304 196 12 288 581 13 294 9.56e-16 81.6
MS.gene033400.t1 AT1G61210 31.008 129 82 5 280 406 132 255 1.77e-11 67.8
MS.gene033400.t1 AT1G15440 26.457 223 156 5 288 506 347 565 9.90e-17 84.7
MS.gene033400.t1 AT1G15440 26.457 223 156 5 288 506 387 605 1.21e-16 84.3
MS.gene033400.t1 AT5G43920 24.688 320 218 12 274 580 204 513 1.69e-16 83.2
MS.gene033400.t1 AT1G11160 24.742 291 195 9 283 561 8 286 3.96e-16 82.8
MS.gene033400.t1 AT1G11160 27.684 177 120 6 281 456 91 260 5.67e-15 79.0
MS.gene033400.t1 AT1G11160 25.397 189 134 4 392 580 30 211 1.42e-14 77.8
MS.gene033400.t1 AT1G11160 30.534 131 80 6 280 406 132 255 5.43e-11 66.2
MS.gene033400.t1 AT1G11160 24.742 291 195 9 283 561 8 286 4.09e-16 82.8
MS.gene033400.t1 AT1G11160 27.684 177 120 6 281 456 91 260 5.82e-15 79.0
MS.gene033400.t1 AT1G11160 25.397 189 134 4 392 580 30 211 1.61e-14 77.8
MS.gene033400.t1 AT1G11160 30.534 131 80 6 280 406 132 255 5.57e-11 66.2
MS.gene033400.t1 AT1G11160 24.742 291 195 9 283 561 8 286 5.17e-16 82.4
MS.gene033400.t1 AT1G11160 27.684 177 120 6 281 456 91 260 6.45e-15 79.0
MS.gene033400.t1 AT1G11160 25.397 189 134 4 392 580 30 211 1.85e-14 77.4
MS.gene033400.t1 AT1G11160 30.534 131 80 6 280 406 132 255 6.41e-11 66.2
MS.gene033400.t1 AT1G11160 27.684 177 120 6 281 456 49 218 4.72e-15 79.3
MS.gene033400.t1 AT1G11160 25.926 162 113 4 419 580 15 169 1.86e-12 70.9
MS.gene033400.t1 AT1G11160 25.000 248 163 8 326 561 8 244 8.79e-12 68.9
MS.gene033400.t1 AT1G11160 30.534 131 80 6 280 406 90 213 4.83e-11 66.6
MS.gene033400.t1 AT3G18130 24.907 269 175 11 287 535 60 321 4.40e-14 74.3
MS.gene033400.t1 AT3G18130 24.907 269 184 9 289 549 14 272 9.64e-12 67.0
MS.gene033400.t1 AT5G52820 24.497 298 204 10 287 580 191 471 2.88e-13 72.8
MS.gene033400.t1 AT1G48630 24.907 269 177 11 287 537 60 321 4.25e-13 71.2
MS.gene033400.t1 AT1G48630 24.542 273 188 9 285 549 10 272 5.75e-12 67.8
MS.gene033400.t1 AT2G33340 25.591 254 173 9 281 528 255 498 4.32e-13 72.4
MS.gene033400.t1 AT2G33340 25.591 254 173 9 281 528 255 498 4.32e-13 72.4
MS.gene033400.t1 AT5G16750 25.444 169 118 5 285 448 55 220 6.54e-13 72.4
MS.gene033400.t1 AT2G41500 23.228 254 179 7 331 580 252 493 1.02e-12 71.2
MS.gene033400.t1 AT2G41500 25.472 212 152 6 282 492 331 537 4.39e-12 69.3
MS.gene033400.t1 AT2G41500 23.228 254 179 7 331 580 252 493 1.02e-12 71.2
MS.gene033400.t1 AT2G41500 25.472 212 152 6 282 492 331 537 4.39e-12 69.3
MS.gene033400.t1 AT1G73720 24.451 319 214 12 279 580 201 509 2.45e-12 70.1
MS.gene033400.t1 AT3G21540 23.864 264 174 12 330 580 402 651 7.33e-12 68.9
MS.gene033400.t1 AT3G21540 27.094 203 134 8 299 494 26 221 4.70e-11 66.6
MS.gene033400.t1 AT2G33340 27.168 173 116 6 281 448 255 422 1.09e-11 67.8
MS.gene033400.t1 AT1G79990 22.460 187 141 3 306 490 72 256 1.20e-11 68.6
MS.gene033400.t1 AT1G18080 23.420 269 182 9 287 537 60 322 1.21e-11 66.6
MS.gene033400.t1 AT1G18080 22.695 282 161 7 285 558 10 242 1.99e-11 66.2
MS.gene033400.t1 AT1G79990 22.460 187 141 3 306 490 72 256 1.29e-11 68.2
MS.gene033400.t1 AT5G60940 22.812 320 225 9 278 580 111 425 7.83e-11 65.1
MS.gene033400.t1 AT5G67320 23.529 357 201 14 278 571 253 600 8.49e-11 65.5
MS.gene033400.t1 AT5G60940 22.812 320 225 9 278 580 19 333 8.60e-11 64.3

Find 168 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAGTCATCATTAGTTTAT+TGG 0.189877 8.4:+22533953 MS.gene033400:CDS
CATTCTCTTCTTTCTTCTTA+AGG 0.216922 8.4:-22532473 None:intergenic
GTGAGGGTAAGTGTTGGTTC+TGG 0.241552 8.4:+22533573 MS.gene033400:CDS
AGCATGGTGTCGTCGACTTT+GGG 0.258974 8.4:-22532093 None:intergenic
ACCCTACCTATGACCAACTA+TGG 0.277536 8.4:+22532187 MS.gene033400:CDS
ATTCTCTTCTTTCTTCTTAA+GGG 0.283276 8.4:-22532472 None:intergenic
CGCTTCACCCCAACGCGAAT+TGG 0.301825 8.4:+22533393 MS.gene033400:CDS
CAAGAGTATGATCAGCATTT+GGG 0.309524 8.4:+22533224 MS.gene033400:CDS
TGGATACTAAGGTTAAGATT+TGG 0.323671 8.4:+22532916 MS.gene033400:CDS
GGATACTAAGGTTAAGATTT+GGG 0.331103 8.4:+22532917 MS.gene033400:CDS
TGATAAGAATATTAAGTATT+GGG 0.340716 8.4:+22533046 MS.gene033400:CDS
GCGGCGAGCCAATTCGCGTT+GGG 0.343990 8.4:-22533401 None:intergenic
CGATATAACCTGCCCTGTTT+CGG 0.352782 8.4:-22533072 None:intergenic
TGAGGGTAAGTGTTGGTTCT+GGG 0.355149 8.4:+22533574 MS.gene033400:CDS
TGGGTCATTCCAAAGCTGTT+AGG 0.358107 8.4:+22532976 MS.gene033400:CDS
TCAAGAGTATGATCAGCATT+TGG 0.358810 8.4:+22533223 MS.gene033400:CDS
TAGCATGGTGTCGTCGACTT+TGG 0.362731 8.4:-22532094 None:intergenic
TCCCTCAGGGTATGGGAGTA+TGG 0.364907 8.4:+22533311 MS.gene033400:CDS
CAAAGAGATTGGTGCATACT+TGG 0.367144 8.4:+22532820 MS.gene033400:CDS
ACATTCTGTTTATCCTCATC+CGG 0.374087 8.4:-22533137 None:intergenic
GATGGGTTTAGAGAGGGTTT+TGG 0.379523 8.4:-22532136 None:intergenic
AAGTATTGGGATACCGAAAC+AGG 0.381659 8.4:+22533059 MS.gene033400:CDS
CAAAAGGGGTTTCTGCGATT+AGG 0.383880 8.4:+22532853 MS.gene033400:CDS
CAAGTATGCACCAATCTCTT+TGG 0.386596 8.4:-22532819 None:intergenic
TTTGCTGGACACATTGTTGC+TGG 0.388967 8.4:+22533494 MS.gene033400:CDS
ACAAATATCCCTAACAGCTT+TGG 0.389574 8.4:-22532985 None:intergenic
GGGTTAAGCTTCACAACATA+AGG 0.392453 8.4:-22533116 None:intergenic
AGAATCAAATGACCCGAATT+CGG 0.398493 8.4:-22532882 None:intergenic
CGTAAGGGTGAGACGGGCCT+TGG 0.399519 8.4:-22532218 None:intergenic
TTACTCGCCTTAGCATCTTT+CGG 0.401503 8.4:-22532780 None:intergenic
GTAAGTGTTGGTTCTGGGAT+TGG 0.407813 8.4:+22533579 MS.gene033400:CDS
CGCGTTGGGGTGAAGCGAAA+TGG 0.409944 8.4:-22533387 None:intergenic
GCAAAGTAGCGACGTGTGGC+TGG 0.411956 8.4:+22533681 MS.gene033400:CDS
ATGATAAGAATATTAAGTAT+TGG 0.420715 8.4:+22533045 MS.gene033400:CDS
TTGTGCTATAAATAAGAATC+TGG 0.429963 8.4:-22533439 None:intergenic
TCTTCTTCTGTTCGCGGTGT+GGG 0.431476 8.4:-22532437 None:intergenic
GGGATTATCAAGGTAGGTCT+TGG 0.431742 8.4:+22532748 MS.gene033400:CDS
CGCGGCGAGCCAATTCGCGT+TGG 0.432970 8.4:-22533402 None:intergenic
ATGACAAGTCCCTCAGGGTA+TGG 0.434880 8.4:+22533303 MS.gene033400:CDS
CTGGATCGTTGTCGGAGTAT+TGG 0.436074 8.4:-22531999 None:intergenic
GAGTCCTTGGGCTGGGAAGA+AGG 0.437077 8.4:+22532581 MS.gene033400:CDS
AATAACCACGCCTCAGAAGC+TGG 0.437350 8.4:-22532546 None:intergenic
TTGGCTCGCCGCGCAGAGCT+TGG 0.439153 8.4:+22533412 MS.gene033400:CDS
CGGTTGCCAGTGGCATCCTC+TGG 0.441347 8.4:+22533652 MS.gene033400:CDS
TGAAGAATAAGAAGAGTCCT+TGG 0.443248 8.4:+22532568 MS.gene033400:CDS
CTCTTCTTCTGTTCGCGGTG+TGG 0.444110 8.4:-22532438 None:intergenic
GGGCGGCAGAACGGGATGGG+AGG 0.448266 8.4:-22532071 None:intergenic
AGAACGGGATGGGAGGAGAC+GGG 0.450151 8.4:-22532064 None:intergenic
CAATACTCCGACAACGATCC+AGG 0.450178 8.4:+22532000 MS.gene033400:CDS
GTTCAGTGGGATATGAATAC+AGG 0.451594 8.4:+22533191 MS.gene033400:CDS
GGGTCATTCCAAAGCTGTTA+GGG 0.454301 8.4:+22532977 MS.gene033400:CDS
GAACGGGATGGGAGGAGACG+GGG 0.455804 8.4:-22532063 None:intergenic
AGGTTTACAAGGAGAGTTAA+CGG 0.457451 8.4:+22532605 MS.gene033400:CDS
TTTGGGGTCGGCTACAGTTA+GGG 0.461852 8.4:-22532118 None:intergenic
GTTATATCGACGTTCTCCAC+TGG 0.463024 8.4:+22533086 MS.gene033400:CDS
TTCGGGTCATTTGATTCTCT+CGG 0.463515 8.4:+22532887 MS.gene033400:CDS
CGGGATGGGAGGAGACGGGG+TGG 0.467337 8.4:-22532060 None:intergenic
GTTTGTGGTTACGCATACCT+TGG 0.469238 8.4:-22532257 None:intergenic
TAGCGACGTGTGGCTGGGAC+GGG 0.469608 8.4:+22533687 MS.gene033400:CDS
GAGGAGCACGCCAAGAAGAA+AGG 0.472310 8.4:+22532648 MS.gene033400:CDS
CTAGTGATGACAAGTCCCTC+AGG 0.475381 8.4:+22533297 MS.gene033400:CDS
TCAACACTGGTAAATGTATG+AGG 0.476160 8.4:+22532946 MS.gene033400:CDS
ACTTGGAGCGGACATACAAA+AGG 0.478707 8.4:+22532837 MS.gene033400:CDS
GGGAGCCCATAGTTGGTCAT+AGG 0.481488 8.4:-22532193 None:intergenic
CTTGGATGGGAGCCCATAGT+TGG 0.481531 8.4:-22532200 None:intergenic
TATTGGTCCAACAGTCTAGC+AGG 0.485881 8.4:+22533970 MS.gene033400:CDS
CAGAACGGGATGGGAGGAGA+CGG 0.487345 8.4:-22532065 None:intergenic
AGGCGACAAGGTGTTGAGTT+GGG 0.487361 8.4:-22532161 None:intergenic
AACTCAAAACCCTGATTCAC+CGG 0.489991 8.4:+22532029 MS.gene033400:CDS
CTTCGACGAAACCAAGTTTG+TGG 0.490052 8.4:-22532272 None:intergenic
CTCGGCTGGTATGGATACTA+AGG 0.491505 8.4:+22532905 MS.gene033400:CDS
TTGGGTCGATGGGTTTAGAG+AGG 0.491971 8.4:-22532143 None:intergenic
TTTGATTCTCTCGGCTGGTA+TGG 0.492749 8.4:+22532896 MS.gene033400:CDS
CATGTCTCCTGCTAGACTGT+TGG 0.495982 8.4:-22533977 None:intergenic
AGGGTGAGACGGGCCTTGGA+TGG 0.498298 8.4:-22532214 None:intergenic
GGTCATTTGATTCTCTCGGC+TGG 0.501830 8.4:+22532891 MS.gene033400:CDS
TTTGTGGTTACGCATACCTT+GGG 0.502829 8.4:-22532256 None:intergenic
TGTTGCTGGATATGCTTGTC+AGG 0.503005 8.4:+22533508 MS.gene033400:CDS
AGGTGTTGAGTTGGGTCGAT+GGG 0.504885 8.4:-22532153 None:intergenic
GGGTGAGACGGGCCTTGGAT+GGG 0.513343 8.4:-22532213 None:intergenic
GCTAAACCGTCTTTAGCGTA+AGG 0.515618 8.4:-22532234 None:intergenic
TGGTGAACCTGGATCGTTGT+CGG 0.515766 8.4:-22532007 None:intergenic
CAAAGTAGCGACGTGTGGCT+GGG 0.520964 8.4:+22533682 MS.gene033400:CDS
CATTCTGTTTATCCTCATCC+GGG 0.523411 8.4:-22533136 None:intergenic
GTAGCGACGTGTGGCTGGGA+CGG 0.525013 8.4:+22533686 MS.gene033400:CDS
TTGGTCATAGGTAGGGTTGA+AGG 0.527966 8.4:-22532181 None:intergenic
CGCGCCTCCGAAAGATGCTA+AGG 0.529557 8.4:+22532773 MS.gene033400:CDS
CTAATATACTTTATAACCAC+GGG 0.530865 8.4:-22533338 None:intergenic
CTTGGAGCGGACATACAAAA+GGG 0.531466 8.4:+22532838 MS.gene033400:CDS
AAGGCGACAAGGTGTTGAGT+TGG 0.535225 8.4:-22532162 None:intergenic
TGTCATGTCGGGAGATGGTG+AGG 0.538558 8.4:+22533556 MS.gene033400:CDS
TGATAAGAAGATTGTTCAGT+GGG 0.538924 8.4:+22533178 MS.gene033400:CDS
ATAAGAAGAGTCCTTGGGCT+GGG 0.539663 8.4:+22532574 MS.gene033400:CDS
GCCCATAGTTGGTCATAGGT+AGG 0.539722 8.4:-22532189 None:intergenic
GATTGAGAATCCAGCTTCTG+AGG 0.541239 8.4:+22532536 MS.gene033400:CDS
TTATGTTGTGAAGCTTAACC+CGG 0.541534 8.4:+22533118 MS.gene033400:CDS
AAGGTGTTGAGTTGGGTCGA+TGG 0.542762 8.4:-22532154 None:intergenic
TGGGTCGATGGGTTTAGAGA+GGG 0.542777 8.4:-22532142 None:intergenic
GGTATGCGTAACCACAAACT+TGG 0.542950 8.4:+22532261 MS.gene033400:CDS
GCATGGTGTCGTCGACTTTG+GGG 0.545386 8.4:-22532092 None:intergenic
GAAGAATAAGAAGAGTCCTT+GGG 0.545960 8.4:+22532569 MS.gene033400:CDS
CAACTATGGGCTCCCATCCA+AGG 0.546220 8.4:+22532201 MS.gene033400:CDS
GTTGTTATCAACAAAAGTAA+TGG 0.548421 8.4:-22533258 None:intergenic
CTTTAGCGTAAGGGTGAGAC+GGG 0.549707 8.4:-22532224 None:intergenic
TGACAAGTCCCTCAGGGTAT+GGG 0.550285 8.4:+22533304 MS.gene033400:CDS
TTATATCGACGTTCTCCACT+GGG 0.551618 8.4:+22533087 MS.gene033400:CDS
CCTTCCTTCTTCCCAGCCCA+AGG 0.553576 8.4:-22532585 None:intergenic
CACACCGCGAACAGAAGAAG+AGG 0.557884 8.4:+22532439 MS.gene033400:CDS
TGCTGTTCATCAAAGAGGAA+AGG 0.560443 8.4:-22532309 None:intergenic
GGGACGGGTTGATAAAGTAC+TGG 0.561357 8.4:+22533702 MS.gene033400:CDS
GGTTAAGCTTCACAACATAA+GGG 0.563836 8.4:-22533115 None:intergenic
CCTTGGGCTGGGAAGAAGGA+AGG 0.564772 8.4:+22532585 MS.gene033400:CDS
CGGCGAGCCAATTCGCGTTG+GGG 0.566063 8.4:-22533400 None:intergenic
GGGAAGAAGGAAGGTTTACA+AGG 0.566983 8.4:+22532594 MS.gene033400:CDS
AATAAGAAGAGTCCTTGGGC+TGG 0.567302 8.4:+22532573 MS.gene033400:CDS
GACATGACAAAACGTCCATC+CGG 0.567963 8.4:-22533542 None:intergenic
CCCATAGTTGGTCATAGGTA+GGG 0.571226 8.4:-22532188 None:intergenic
AAAGAGGAAAGGGATTATCA+AGG 0.572402 8.4:+22532738 MS.gene033400:CDS
TTGGGATACCGAAACAGGGC+AGG 0.573233 8.4:+22533064 MS.gene033400:CDS
GGCTACAGTTAGGGTTAGCA+TGG 0.574144 8.4:-22532109 None:intergenic
AGATTCTTATTTATAGCACA+AGG 0.575025 8.4:+22533441 MS.gene033400:CDS
GAAGGCATAGAGTGCATATG+TGG 0.575203 8.4:-22533365 None:intergenic
AAGAGTATGATCAGCATTTG+GGG 0.578509 8.4:+22533225 MS.gene033400:CDS
TGTGAAGCTTAACCCGGATG+AGG 0.580826 8.4:+22533124 MS.gene033400:CDS
TGTCACGAAGGCGTTACCAT+CGG 0.581186 8.4:+22533632 MS.gene033400:CDS
TAAATGTATGAGGACTTACA+TGG 0.581377 8.4:+22532956 MS.gene033400:CDS
AGCAGCGTTATTCTTCTGCA+AGG 0.583382 8.4:-22532400 None:intergenic
AGAATCCAGCTTCTGAGGCG+TGG 0.583927 8.4:+22532541 MS.gene033400:CDS
ACTAATATACTTTATAACCA+CGG 0.592566 8.4:-22533339 None:intergenic
GCTAAAGACGGTTTAGCCCA+AGG 0.595353 8.4:+22532240 MS.gene033400:CDS
CTCGCCTTAGCATCTTTCGG+AGG 0.597269 8.4:-22532777 None:intergenic
GCTACTTTGCTCTGTTCCAG+AGG 0.598337 8.4:-22533668 None:intergenic
GACGAAAATAACGACGAAGA+GGG 0.600243 8.4:+22532507 MS.gene033400:CDS
TGTTATGCTGTTCATCAAAG+AGG 0.601244 8.4:-22532314 None:intergenic
CCCTACCTATGACCAACTAT+GGG 0.603332 8.4:+22532188 MS.gene033400:CDS
TCTGTTCCAGAGGATGCCAC+TGG 0.603366 8.4:-22533658 None:intergenic
CATGGTGTCGTCGACTTTGG+GGG 0.604119 8.4:-22532091 None:intergenic
AGGAAAGGGATTATCAAGGT+AGG 0.608842 8.4:+22532742 MS.gene033400:CDS
CTAAACCGTCTTTAGCGTAA+GGG 0.612580 8.4:-22532233 None:intergenic
TTGGGGTGAAGCGAAATGGA+AGG 0.612660 8.4:-22533383 None:intergenic
TCTTTAGCGTAAGGGTGAGA+CGG 0.615894 8.4:-22532225 None:intergenic
GTCATGTCGGGAGATGGTGA+GGG 0.616926 8.4:+22533557 MS.gene033400:CDS
TTCAGTGGGATATGAATACA+GGG 0.624256 8.4:+22533192 MS.gene033400:CDS
AGGTAGGGTTGAAGGCGACA+AGG 0.626351 8.4:-22532173 None:intergenic
ACCATACTCCCATACCCTGA+GGG 0.627909 8.4:-22533312 None:intergenic
TAGTGATGACAAGTCCCTCA+GGG 0.628378 8.4:+22533298 MS.gene033400:CDS
AGGATGCCACTGGCAACCGA+TGG 0.645652 8.4:-22533648 None:intergenic
TCTCACCCTTACGCTAAAGA+CGG 0.646041 8.4:+22532228 MS.gene033400:CDS
AGATTGGTGCATACTTGGAG+CGG 0.649087 8.4:+22532825 MS.gene033400:CDS
CCGATGTAATTTGACGCAGA+AGG 0.651218 8.4:-22532366 None:intergenic
GCGTTACCATCGGTTGCCAG+TGG 0.652062 8.4:+22533642 MS.gene033400:CDS
GATTCTTATTTATAGCACAA+GGG 0.653583 8.4:+22533442 MS.gene033400:CDS
GAGATGGTGAGGGTAAGTGT+TGG 0.656084 8.4:+22533567 MS.gene033400:CDS
CAGAGCAAAGTAGCGACGTG+TGG 0.656241 8.4:+22533677 MS.gene033400:CDS
CGACGAAAATAACGACGAAG+AGG 0.657011 8.4:+22532506 MS.gene033400:CDS
GTGATAAGAAGATTGTTCAG+TGG 0.659340 8.4:+22533177 MS.gene033400:CDS
CCTTCTGCGTCAAATTACAT+CGG 0.670057 8.4:+22532366 MS.gene033400:CDS
GTCTAGCAGGAGACATGTGA+AGG 0.678980 8.4:+22533983 MS.gene033400:CDS
AAATGTATGAGGACTTACAT+GGG 0.686630 8.4:+22532957 MS.gene033400:CDS
ATGGGAGTATGGTATTCCCG+TGG 0.688322 8.4:+22533322 MS.gene033400:CDS
TTGGAGCGGACATACAAAAG+GGG 0.692861 8.4:+22532839 MS.gene033400:CDS
AGAACTCTCAAGTGTCACGA+AGG 0.693137 8.4:+22533620 MS.gene033400:CDS
AGTATTGGGATACCGAAACA+GGG 0.695845 8.4:+22533060 MS.gene033400:CDS
TACCATACTCCCATACCCTG+AGG 0.704189 8.4:-22533313 None:intergenic
AGAGTATGATCAGCATTTGG+GGG 0.704600 8.4:+22533226 MS.gene033400:CDS
AACATAAGGGATCTTCCCAG+TGG 0.720474 8.4:-22533102 None:intergenic
AGATCAGAAGAAATACGCTG+AGG 0.728271 8.4:+22532629 MS.gene033400:CDS
CTGGTTATCCAAGCTCTGCG+CGG 0.754282 8.4:-22533420 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAAGAATATTAAGTAT+TGG + chr8.4:22533045-22533064 MS.gene033400:CDS 15.0%
!! TGATAAGAATATTAAGTATT+GGG + chr8.4:22533046-22533065 MS.gene033400:CDS 15.0%
!! ACTAATATACTTTATAACCA+CGG - chr8.4:22533342-22533361 None:intergenic 20.0%
!! TAAGACTCTGTAATTATATA+TGG - chr8.4:22533749-22533768 None:intergenic 20.0%
!! TACACAAAATTCTATCAAAA+TGG - chr8.4:22533839-22533858 None:intergenic 20.0%
!! TATTCAATTCAAGCTAATAT+AGG + chr8.4:22533863-22533882 MS.gene033400:intron 20.0%
!! TTAGATTGCATCAAATTATA+GGG + chr8.4:22533926-22533945 MS.gene033400:intron 20.0%
!! TTTAGATTGCATCAAATTAT+AGG + chr8.4:22533925-22533944 MS.gene033400:intron 20.0%
!!! CATAACACCTTTTTAAAATT+CGG + chr8.4:22532330-22532349 MS.gene033400:CDS 20.0%
!!! GATATTTGTTTTACTAATGA+TGG + chr8.4:22532999-22533018 MS.gene033400:CDS 20.0%
!!! TTTTGATAGAATTTTGTGTA+TGG + chr8.4:22533839-22533858 MS.gene033400:intron 20.0%
! ATTCTCTTCTTTCTTCTTAA+GGG - chr8.4:22532475-22532494 None:intergenic 25.0%
! CTAATATACTTTATAACCAC+GGG - chr8.4:22533341-22533360 None:intergenic 25.0%
! GTTGTTATCAACAAAAGTAA+TGG - chr8.4:22533261-22533280 None:intergenic 25.0%
! TTCAACTTAACAAAAAGAAG+AGG + chr8.4:22533471-22533490 MS.gene033400:CDS 25.0%
! TTGTGCTATAAATAAGAATC+TGG - chr8.4:22533442-22533461 None:intergenic 25.0%
!! AGATTCTTATTTATAGCACA+AGG + chr8.4:22533441-22533460 MS.gene033400:CDS 25.0%
!! GATAGTCATCATTAGTTTAT+TGG + chr8.4:22533953-22533972 MS.gene033400:CDS 25.0%
!! GATTCTTATTTATAGCACAA+GGG + chr8.4:22533442-22533461 MS.gene033400:CDS 25.0%
!!! TTACTTTTGTTGATAACAAC+AGG + chr8.4:22533261-22533280 MS.gene033400:CDS 25.0%
!!! TTGTTTTATACCAAAGAGAT+TGG + chr8.4:22532809-22532828 MS.gene033400:CDS 25.0%
AAATGTATGAGGACTTACAT+GGG + chr8.4:22532957-22532976 MS.gene033400:CDS 30.0%
CATTCTCTTCTTTCTTCTTA+AGG - chr8.4:22532476-22532495 None:intergenic 30.0%
GGATACTAAGGTTAAGATTT+GGG + chr8.4:22532917-22532936 MS.gene033400:CDS 30.0%
TAAATGTATGAGGACTTACA+TGG + chr8.4:22532956-22532975 MS.gene033400:CDS 30.0%
TGATAAGAAGATTGTTCAGT+GGG + chr8.4:22533178-22533197 MS.gene033400:CDS 30.0%
TGGATACTAAGGTTAAGATT+TGG + chr8.4:22532916-22532935 MS.gene033400:CDS 30.0%
! GGATAAACAGAATGTTTTGT+TGG + chr8.4:22533145-22533164 MS.gene033400:CDS 30.0%
!! AGCGTAACCGAATTTTAAAA+AGG - chr8.4:22532340-22532359 None:intergenic 30.0%
!!! AAGGATAAGAGTACTTTTCA+TGG + chr8.4:22532714-22532733 MS.gene033400:CDS 30.0%
!!! AGGATAAGAGTACTTTTCAT+GGG + chr8.4:22532715-22532734 MS.gene033400:CDS 30.0%
!!! ATTAGGTTTTTTCCGAATTC+GGG + chr8.4:22532870-22532889 MS.gene033400:CDS 30.0%
!!! GATTAGGTTTTTTCCGAATT+CGG + chr8.4:22532869-22532888 MS.gene033400:CDS 30.0%
AAAGAGGAAAGGGATTATCA+AGG + chr8.4:22532738-22532757 MS.gene033400:CDS 35.0%
AACAAAAAGAAGAGGTTTGC+TGG + chr8.4:22533479-22533498 MS.gene033400:CDS 35.0%
AAGAAAGGCGAAGAAAAAAG+TGG + chr8.4:22532663-22532682 MS.gene033400:CDS 35.0%
ACAAATATCCCTAACAGCTT+TGG - chr8.4:22532988-22533007 None:intergenic 35.0%
ACATTCTGTTTATCCTCATC+CGG - chr8.4:22533140-22533159 None:intergenic 35.0%
AGAATCAAATGACCCGAATT+CGG - chr8.4:22532885-22532904 None:intergenic 35.0%
AGGTTTACAAGGAGAGTTAA+CGG + chr8.4:22532605-22532624 MS.gene033400:CDS 35.0%
GAAGAATAAGAAGAGTCCTT+GGG + chr8.4:22532569-22532588 MS.gene033400:CDS 35.0%
GGTTAAGCTTCACAACATAA+GGG - chr8.4:22533118-22533137 None:intergenic 35.0%
GTGATAAGAAGATTGTTCAG+TGG + chr8.4:22533177-22533196 MS.gene033400:CDS 35.0%
TCAACACTGGTAAATGTATG+AGG + chr8.4:22532946-22532965 MS.gene033400:CDS 35.0%
TGAAAAAGTTGAGGCTGTTA+AGG + chr8.4:22532695-22532714 MS.gene033400:CDS 35.0%
TGAAGAATAAGAAGAGTCCT+TGG + chr8.4:22532568-22532587 MS.gene033400:CDS 35.0%
TGTTATGCTGTTCATCAAAG+AGG - chr8.4:22532317-22532336 None:intergenic 35.0%
TTATGTTGTGAAGCTTAACC+CGG + chr8.4:22533118-22533137 MS.gene033400:CDS 35.0%
TTCAGTGGGATATGAATACA+GGG + chr8.4:22533192-22533211 MS.gene033400:CDS 35.0%
! AAGAGTATGATCAGCATTTG+GGG + chr8.4:22533225-22533244 MS.gene033400:CDS 35.0%
! CAAGAGTATGATCAGCATTT+GGG + chr8.4:22533224-22533243 MS.gene033400:CDS 35.0%
! TCAAGAGTATGATCAGCATT+TGG + chr8.4:22533223-22533242 MS.gene033400:CDS 35.0%
! TTTTCATGGGAAAGAGGAAA+GGG + chr8.4:22532728-22532747 MS.gene033400:CDS 35.0%
! TTTTTCTTCGCCTTTCTTCT+TGG - chr8.4:22532661-22532680 None:intergenic 35.0%
!! AAACAGAATGTTTTGTTGGC+TGG + chr8.4:22533149-22533168 MS.gene033400:CDS 35.0%
!! ATTTGGGATGTTTTCAACAC+TGG + chr8.4:22532933-22532952 MS.gene033400:CDS 35.0%
!!! GAAGAAAAAAGTGGTTTTGG+TGG + chr8.4:22532672-22532691 MS.gene033400:CDS 35.0%
!!! TTTTGGTGGTGAAAAAGTTG+AGG + chr8.4:22532686-22532705 MS.gene033400:CDS 35.0%
AACTCAAAACCCTGATTCAC+CGG + chr8.4:22532029-22532048 MS.gene033400:CDS 40.0%
AGAGTATGATCAGCATTTGG+GGG + chr8.4:22533226-22533245 MS.gene033400:CDS 40.0%
AGATCAGAAGAAATACGCTG+AGG + chr8.4:22532629-22532648 MS.gene033400:CDS 40.0%
AGGAAAGGGATTATCAAGGT+AGG + chr8.4:22532742-22532761 MS.gene033400:CDS 40.0%
CAAAGAGATTGGTGCATACT+TGG + chr8.4:22532820-22532839 MS.gene033400:CDS 40.0%
CAAGTATGCACCAATCTCTT+TGG - chr8.4:22532822-22532841 None:intergenic 40.0%
CATTCTGTTTATCCTCATCC+GGG - chr8.4:22533139-22533158 None:intergenic 40.0%
CCTTCTGCGTCAAATTACAT+CGG + chr8.4:22532366-22532385 MS.gene033400:CDS 40.0%
CTAAACCGTCTTTAGCGTAA+GGG - chr8.4:22532236-22532255 None:intergenic 40.0%
GACGAAAATAACGACGAAGA+GGG + chr8.4:22532507-22532526 MS.gene033400:CDS 40.0%
GGGTTAAGCTTCACAACATA+AGG - chr8.4:22533119-22533138 None:intergenic 40.0%
GTTCAGTGGGATATGAATAC+AGG + chr8.4:22533191-22533210 MS.gene033400:CDS 40.0%
TGCTGTTCATCAAAGAGGAA+AGG - chr8.4:22532312-22532331 None:intergenic 40.0%
TTACTCGCCTTAGCATCTTT+CGG - chr8.4:22532783-22532802 None:intergenic 40.0%
TTATATCGACGTTCTCCACT+GGG + chr8.4:22533087-22533106 MS.gene033400:CDS 40.0%
TTCGGGTCATTTGATTCTCT+CGG + chr8.4:22532887-22532906 MS.gene033400:CDS 40.0%
TTTGTGGTTACGCATACCTT+GGG - chr8.4:22532259-22532278 None:intergenic 40.0%
! AGTATTGGGATACCGAAACA+GGG + chr8.4:22533060-22533079 MS.gene033400:CDS 40.0%
! ATGGGTTTAGAGAGGGTTTT+GGG - chr8.4:22532138-22532157 None:intergenic 40.0%
! CTTTTCATGGGAAAGAGGAA+AGG + chr8.4:22532727-22532746 MS.gene033400:CDS 40.0%
! GAGAATTTTCCGGTGAATCA+GGG - chr8.4:22532041-22532060 None:intergenic 40.0%
! GAGTACTTTTCATGGGAAAG+AGG + chr8.4:22532722-22532741 MS.gene033400:CDS 40.0%
! GGCGAAGAAAAAAGTGGTTT+TGG + chr8.4:22532669-22532688 MS.gene033400:CDS 40.0%
! TTGTCAGGTCAATTTTTCGC+CGG + chr8.4:22533523-22533542 MS.gene033400:CDS 40.0%
! TTTTCCTCTTCTTCTGTTCG+CGG - chr8.4:22532446-22532465 None:intergenic 40.0%
!! AAGTATTGGGATACCGAAAC+AGG + chr8.4:22533059-22533078 MS.gene033400:CDS 40.0%
!!! GGAACAAAGTTTTTGAGTGC+TGG + chr8.4:22533020-22533039 MS.gene033400:CDS 40.0%
AACATAAGGGATCTTCCCAG+TGG - chr8.4:22533105-22533124 None:intergenic 45.0%
AATAAGAAGAGTCCTTGGGC+TGG + chr8.4:22532573-22532592 MS.gene033400:CDS 45.0%
ACCCTACCTATGACCAACTA+TGG + chr8.4:22532187-22532206 MS.gene033400:CDS 45.0%
ACTTGGAGCGGACATACAAA+AGG + chr8.4:22532837-22532856 MS.gene033400:CDS 45.0%
AGAACTCTCAAGTGTCACGA+AGG + chr8.4:22533620-22533639 MS.gene033400:CDS 45.0%
AGATTGGTGCATACTTGGAG+CGG + chr8.4:22532825-22532844 MS.gene033400:CDS 45.0%
AGCAGCGTTATTCTTCTGCA+AGG - chr8.4:22532403-22532422 None:intergenic 45.0%
ATAAGAAGAGTCCTTGGGCT+GGG + chr8.4:22532574-22532593 MS.gene033400:CDS 45.0%
CAAAAGGGGTTTCTGCGATT+AGG + chr8.4:22532853-22532872 MS.gene033400:CDS 45.0%
CCCATAGTTGGTCATAGGTA+GGG - chr8.4:22532191-22532210 None:intergenic 45.0%
CCCTACCTATGACCAACTAT+GGG + chr8.4:22532188-22532207 MS.gene033400:CDS 45.0%
CCGATGTAATTTGACGCAGA+AGG - chr8.4:22532369-22532388 None:intergenic 45.0%
CGACGAAAATAACGACGAAG+AGG + chr8.4:22532506-22532525 MS.gene033400:CDS 45.0%
CGATATAACCTGCCCTGTTT+CGG - chr8.4:22533075-22533094 None:intergenic 45.0%
CTTCGACGAAACCAAGTTTG+TGG - chr8.4:22532275-22532294 None:intergenic 45.0%
CTTGGAGCGGACATACAAAA+GGG + chr8.4:22532838-22532857 MS.gene033400:CDS 45.0%
GACATGACAAAACGTCCATC+CGG - chr8.4:22533545-22533564 None:intergenic 45.0%
GATTGAGAATCCAGCTTCTG+AGG + chr8.4:22532536-22532555 MS.gene033400:CDS 45.0%
GCAATGAGTAACTGACAGTG+AGG - chr8.4:22533798-22533817 None:intergenic 45.0%
GCTAAACCGTCTTTAGCGTA+AGG - chr8.4:22532237-22532256 None:intergenic 45.0%
GGGAAGAAGGAAGGTTTACA+AGG + chr8.4:22532594-22532613 MS.gene033400:CDS 45.0%
GGGATTATCAAGGTAGGTCT+TGG + chr8.4:22532748-22532767 MS.gene033400:CDS 45.0%
GGGTCATTCCAAAGCTGTTA+GGG + chr8.4:22532977-22532996 MS.gene033400:CDS 45.0%
GGTATGCGTAACCACAAACT+TGG + chr8.4:22532261-22532280 MS.gene033400:CDS 45.0%
GTTATATCGACGTTCTCCAC+TGG + chr8.4:22533086-22533105 MS.gene033400:CDS 45.0%
GTTTGTGGTTACGCATACCT+TGG - chr8.4:22532260-22532279 None:intergenic 45.0%
TAGTGATGACAAGTCCCTCA+GGG + chr8.4:22533298-22533317 MS.gene033400:CDS 45.0%
TATTGGTCCAACAGTCTAGC+AGG + chr8.4:22533970-22533989 MS.gene033400:CDS 45.0%
TCTCACCCTTACGCTAAAGA+CGG + chr8.4:22532228-22532247 MS.gene033400:CDS 45.0%
TCTTTAGCGTAAGGGTGAGA+CGG - chr8.4:22532228-22532247 None:intergenic 45.0%
TGGGTCATTCCAAAGCTGTT+AGG + chr8.4:22532976-22532995 MS.gene033400:CDS 45.0%
TGTTGCTGGATATGCTTGTC+AGG + chr8.4:22533508-22533527 MS.gene033400:CDS 45.0%
TTGGAGCGGACATACAAAAG+GGG + chr8.4:22532839-22532858 MS.gene033400:CDS 45.0%
TTGGTCATAGGTAGGGTTGA+AGG - chr8.4:22532184-22532203 None:intergenic 45.0%
TTTGATTCTCTCGGCTGGTA+TGG + chr8.4:22532896-22532915 MS.gene033400:CDS 45.0%
TTTGCTGGACACATTGTTGC+TGG + chr8.4:22533494-22533513 MS.gene033400:CDS 45.0%
! GAAGGCATAGAGTGCATATG+TGG - chr8.4:22533368-22533387 None:intergenic 45.0%
! GATGGACGTTTTGTCATGTC+GGG + chr8.4:22533545-22533564 MS.gene033400:CDS 45.0%
! GGAGAATTTTCCGGTGAATC+AGG - chr8.4:22532042-22532061 None:intergenic 45.0%
! GGATGGACGTTTTGTCATGT+CGG + chr8.4:22533544-22533563 MS.gene033400:CDS 45.0%
! TGGGTTTAGAGAGGGTTTTG+GGG - chr8.4:22532137-22532156 None:intergenic 45.0%
!! GATGGGTTTAGAGAGGGTTT+TGG - chr8.4:22532139-22532158 None:intergenic 45.0%
!! GTAAGTGTTGGTTCTGGGAT+TGG + chr8.4:22533579-22533598 MS.gene033400:CDS 45.0%
!! TGAGGGTAAGTGTTGGTTCT+GGG + chr8.4:22533574-22533593 MS.gene033400:CDS 45.0%
!!! GGTGAATCAGGGTTTTGAGT+TGG - chr8.4:22532030-22532049 None:intergenic 45.0%
!!! TTTAGAGAGGGTTTTGGGGT+CGG - chr8.4:22532133-22532152 None:intergenic 45.0%
AATAACCACGCCTCAGAAGC+TGG - chr8.4:22532549-22532568 None:intergenic 50.0%
ACCATACTCCCATACCCTGA+GGG - chr8.4:22533315-22533334 None:intergenic 50.0%
ATGACAAGTCCCTCAGGGTA+TGG + chr8.4:22533303-22533322 MS.gene033400:CDS 50.0%
CAATACTCCGACAACGATCC+AGG + chr8.4:22532000-22532019 MS.gene033400:CDS 50.0%
CATGTCTCCTGCTAGACTGT+TGG - chr8.4:22533980-22533999 None:intergenic 50.0%
CTAGTGATGACAAGTCCCTC+AGG + chr8.4:22533297-22533316 MS.gene033400:CDS 50.0%
CTCGGCTGGTATGGATACTA+AGG + chr8.4:22532905-22532924 MS.gene033400:CDS 50.0%
CTGGATCGTTGTCGGAGTAT+TGG - chr8.4:22532002-22532021 None:intergenic 50.0%
CTTTAGCGTAAGGGTGAGAC+GGG - chr8.4:22532227-22532246 None:intergenic 50.0%
GAGATGGTGAGGGTAAGTGT+TGG + chr8.4:22533567-22533586 MS.gene033400:CDS 50.0%
GCCCATAGTTGGTCATAGGT+AGG - chr8.4:22532192-22532211 None:intergenic 50.0%
GCTAAAGACGGTTTAGCCCA+AGG + chr8.4:22532240-22532259 MS.gene033400:CDS 50.0%
GGTCATTTGATTCTCTCGGC+TGG + chr8.4:22532891-22532910 MS.gene033400:CDS 50.0%
GTCTAGCAGGAGACATGTGA+AGG + chr8.4:22533983-22534002 MS.gene033400:CDS 50.0%
TACCATACTCCCATACCCTG+AGG - chr8.4:22533316-22533335 None:intergenic 50.0%
TCTTCTTCTGTTCGCGGTGT+GGG - chr8.4:22532440-22532459 None:intergenic 50.0%
TGACAAGTCCCTCAGGGTAT+GGG + chr8.4:22533304-22533323 MS.gene033400:CDS 50.0%
TGGTGAACCTGGATCGTTGT+CGG - chr8.4:22532010-22532029 None:intergenic 50.0%
TGTGAAGCTTAACCCGGATG+AGG + chr8.4:22533124-22533143 MS.gene033400:CDS 50.0%
TTGGGGTGAAGCGAAATGGA+AGG - chr8.4:22533386-22533405 None:intergenic 50.0%
! AGACGGGGTGGAGAATTTTC+CGG - chr8.4:22532051-22532070 None:intergenic 50.0%
! ATGGGAGTATGGTATTCCCG+TGG + chr8.4:22533322-22533341 MS.gene033400:CDS 50.0%
! CAGGTCAATTTTTCGCCGGA+TGG + chr8.4:22533527-22533546 MS.gene033400:CDS 50.0%
! CGTTTTGTCATGTCGGGAGA+TGG + chr8.4:22533551-22533570 MS.gene033400:CDS 50.0%
! GCTACTTTGCTCTGTTCCAG+AGG - chr8.4:22533671-22533690 None:intergenic 50.0%
! GGCTACAGTTAGGGTTAGCA+TGG - chr8.4:22532112-22532131 None:intergenic 50.0%
! TGGGTCGATGGGTTTAGAGA+GGG - chr8.4:22532145-22532164 None:intergenic 50.0%
! TTGGGTCGATGGGTTTAGAG+AGG - chr8.4:22532146-22532165 None:intergenic 50.0%
! TTTGGGGTCGGCTACAGTTA+GGG - chr8.4:22532121-22532140 None:intergenic 50.0%
!! AAGGCGACAAGGTGTTGAGT+TGG - chr8.4:22532165-22532184 None:intergenic 50.0%
!! AAGGTGTTGAGTTGGGTCGA+TGG - chr8.4:22532157-22532176 None:intergenic 50.0%
!! AGCATGGTGTCGTCGACTTT+GGG - chr8.4:22532096-22532115 None:intergenic 50.0%
!! AGGCGACAAGGTGTTGAGTT+GGG - chr8.4:22532164-22532183 None:intergenic 50.0%
!! AGGTGTTGAGTTGGGTCGAT+GGG - chr8.4:22532156-22532175 None:intergenic 50.0%
!! GGGACGGGTTGATAAAGTAC+TGG + chr8.4:22533702-22533721 MS.gene033400:CDS 50.0%
!! GTGAGGGTAAGTGTTGGTTC+TGG + chr8.4:22533573-22533592 MS.gene033400:CDS 50.0%
!! TAGCATGGTGTCGTCGACTT+TGG - chr8.4:22532097-22532116 None:intergenic 50.0%
!! TGTCACGAAGGCGTTACCAT+CGG + chr8.4:22533632-22533651 MS.gene033400:CDS 50.0%
!!! GGGTTTTGAGTTGGTGAACC+TGG - chr8.4:22532021-22532040 None:intergenic 50.0%
!!! TTTTGGGGTCGGCTACAGTT+AGG - chr8.4:22532122-22532141 None:intergenic 50.0%
AGGTAGGGTTGAAGGCGACA+AGG - chr8.4:22532176-22532195 None:intergenic 55.0%
CAAAGTAGCGACGTGTGGCT+GGG + chr8.4:22533682-22533701 MS.gene033400:CDS 55.0%
CAACTATGGGCTCCCATCCA+AGG + chr8.4:22532201-22532220 MS.gene033400:CDS 55.0%
CACACCGCGAACAGAAGAAG+AGG + chr8.4:22532439-22532458 MS.gene033400:CDS 55.0%
CAGAGCAAAGTAGCGACGTG+TGG + chr8.4:22533677-22533696 MS.gene033400:CDS 55.0%
CTCGCCTTAGCATCTTTCGG+AGG - chr8.4:22532780-22532799 None:intergenic 55.0%
CTCTTCTTCTGTTCGCGGTG+TGG - chr8.4:22532441-22532460 None:intergenic 55.0%
CTGGTTATCCAAGCTCTGCG+CGG - chr8.4:22533423-22533442 None:intergenic 55.0%
CTTGGATGGGAGCCCATAGT+TGG - chr8.4:22532203-22532222 None:intergenic 55.0%
GGGAGCCCATAGTTGGTCAT+AGG - chr8.4:22532196-22532215 None:intergenic 55.0%
GTCATGTCGGGAGATGGTGA+GGG + chr8.4:22533557-22533576 MS.gene033400:CDS 55.0%
TCCCTCAGGGTATGGGAGTA+TGG + chr8.4:22533311-22533330 MS.gene033400:CDS 55.0%
TCTGTTCCAGAGGATGCCAC+TGG - chr8.4:22533661-22533680 None:intergenic 55.0%
TGTCATGTCGGGAGATGGTG+AGG + chr8.4:22533556-22533575 MS.gene033400:CDS 55.0%
TTGGGATACCGAAACAGGGC+AGG + chr8.4:22533064-22533083 MS.gene033400:CDS 55.0%
! GAGGAGCACGCCAAGAAGAA+AGG + chr8.4:22532648-22532667 MS.gene033400:CDS 55.0%
!! AGAATCCAGCTTCTGAGGCG+TGG + chr8.4:22532541-22532560 MS.gene033400:CDS 55.0%
!! CATGGTGTCGTCGACTTTGG+GGG - chr8.4:22532094-22532113 None:intergenic 55.0%
!! GCATGGTGTCGTCGACTTTG+GGG - chr8.4:22532095-22532114 None:intergenic 55.0%
AGAACGGGATGGGAGGAGAC+GGG - chr8.4:22532067-22532086 None:intergenic 60.0%
AGGATGCCACTGGCAACCGA+TGG - chr8.4:22533651-22533670 None:intergenic 60.0%
CAGAACGGGATGGGAGGAGA+CGG - chr8.4:22532068-22532087 None:intergenic 60.0%
CCTTCCTTCTTCCCAGCCCA+AGG - chr8.4:22532588-22532607 None:intergenic 60.0%
CGCGCCTCCGAAAGATGCTA+AGG + chr8.4:22532773-22532792 MS.gene033400:CDS 60.0%
CGCGTTGGGGTGAAGCGAAA+TGG - chr8.4:22533390-22533409 None:intergenic 60.0%
CGCTTCACCCCAACGCGAAT+TGG + chr8.4:22533393-22533412 MS.gene033400:CDS 60.0%
GCAAAGTAGCGACGTGTGGC+TGG + chr8.4:22533681-22533700 MS.gene033400:CDS 60.0%
! CCTTGGGCTGGGAAGAAGGA+AGG + chr8.4:22532585-22532604 MS.gene033400:CDS 60.0%
! GAGTCCTTGGGCTGGGAAGA+AGG + chr8.4:22532581-22532600 MS.gene033400:CDS 60.0%
! GCGTTACCATCGGTTGCCAG+TGG + chr8.4:22533642-22533661 MS.gene033400:CDS 60.0%
AGGGTGAGACGGGCCTTGGA+TGG - chr8.4:22532217-22532236 None:intergenic 65.0%
CGGCGAGCCAATTCGCGTTG+GGG - chr8.4:22533403-22533422 None:intergenic 65.0%
CGGTTGCCAGTGGCATCCTC+TGG + chr8.4:22533652-22533671 MS.gene033400:CDS 65.0%
CGTAAGGGTGAGACGGGCCT+TGG - chr8.4:22532221-22532240 None:intergenic 65.0%
GAACGGGATGGGAGGAGACG+GGG - chr8.4:22532066-22532085 None:intergenic 65.0%
GCGGCGAGCCAATTCGCGTT+GGG - chr8.4:22533404-22533423 None:intergenic 65.0%
GGGTGAGACGGGCCTTGGAT+GGG - chr8.4:22532216-22532235 None:intergenic 65.0%
GTAGCGACGTGTGGCTGGGA+CGG + chr8.4:22533686-22533705 MS.gene033400:CDS 65.0%
TAGCGACGTGTGGCTGGGAC+GGG + chr8.4:22533687-22533706 MS.gene033400:CDS 65.0%
! CGACTTTGGGGGCGGCAGAA+CGG - chr8.4:22532083-22532102 None:intergenic 65.0%
! GACTTTGGGGGCGGCAGAAC+GGG - chr8.4:22532082-22532101 None:intergenic 65.0%
!! GGTGTCGTCGACTTTGGGGG+CGG - chr8.4:22532091-22532110 None:intergenic 65.0%
CGCGGCGAGCCAATTCGCGT+TGG - chr8.4:22533405-22533424 None:intergenic 70.0%
TGGGGGCGGCAGAACGGGAT+GGG - chr8.4:22532077-22532096 None:intergenic 70.0%
TTGGGGGCGGCAGAACGGGA+TGG - chr8.4:22532078-22532097 None:intergenic 70.0%
! TTGGCTCGCCGCGCAGAGCT+TGG + chr8.4:22533412-22533431 MS.gene033400:CDS 70.0%
CGGGATGGGAGGAGACGGGG+TGG - chr8.4:22532063-22532082 None:intergenic 75.0%
GGGCGGCAGAACGGGATGGG+AGG - chr8.4:22532074-22532093 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 22531985 22534013 22531985 ID=MS.gene033400
chr8.4 mRNA 22531985 22534013 22531985 ID=MS.gene033400.t1;Parent=MS.gene033400
chr8.4 exon 22531985 22533723 22531985 ID=MS.gene033400.t1.exon1;Parent=MS.gene033400.t1
chr8.4 CDS 22531985 22533723 22531985 ID=cds.MS.gene033400.t1;Parent=MS.gene033400.t1
chr8.4 exon 22533947 22534013 22533947 ID=MS.gene033400.t1.exon2;Parent=MS.gene033400.t1
chr8.4 CDS 22533947 22534013 22533947 ID=cds.MS.gene033400.t1;Parent=MS.gene033400.t1
Gene Sequence

>MS.gene033400

ATGGATCTACTTAACCAATACTCCGACAACGATCCAGGTTCACCAACTCAAAACCCTGATTCACCGGAAAATTCTCCACCCCGTCTCCTCCCATCCCGTTCTGCCGCCCCCAAAGTCGACGACACCATGCTAACCCTAACTGTAGCCGACCCCAAAACCCTCTCTAAACCCATCGACCCAACTCAACACCTTGTCGCCTTCAACCCTACCTATGACCAACTATGGGCTCCCATCCAAGGCCCGTCTCACCCTTACGCTAAAGACGGTTTAGCCCAAGGTATGCGTAACCACAAACTTGGTTTCGTCGAAGATGCTAACATTGAACCTTTCCTCTTTGATGAACAGCATAACACCTTTTTAAAATTCGGTTACGCTGCTGATCCTTCTGCGTCAAATTACATCGGTGATTTCGATGCCTTGCAGAAGAATAACGCTGCTTCTGTTTACAATATCCCACACCGCGAACAGAAGAAGAGGAAAATTGAATCCCTTAAGAAGAAAGAAGAGAATGATGATGAAGACGACGAAAATAACGACGAAGAGGGTGTTGAGATTGAGAATCCAGCTTCTGAGGCGTGGTTATTGAAGAATAAGAAGAGTCCTTGGGCTGGGAAGAAGGAAGGTTTACAAGGAGAGTTAACGGAAGATCAGAAGAAATACGCTGAGGAGCACGCCAAGAAGAAAGGCGAAGAAAAAAGTGGTTTTGGTGGTGAAAAAGTTGAGGCTGTTAAGGATAAGAGTACTTTTCATGGGAAAGAGGAAAGGGATTATCAAGGTAGGTCTTGGATCGCGCCTCCGAAAGATGCTAAGGCGAGTAATGATCATTGTTTTATACCAAAGAGATTGGTGCATACTTGGAGCGGACATACAAAAGGGGTTTCTGCGATTAGGTTTTTTCCGAATTCGGGTCATTTGATTCTCTCGGCTGGTATGGATACTAAGGTTAAGATTTGGGATGTTTTCAACACTGGTAAATGTATGAGGACTTACATGGGTCATTCCAAAGCTGTTAGGGATATTTGTTTTACTAATGATGGAACAAAGTTTTTGAGTGCTGGTTATGATAAGAATATTAAGTATTGGGATACCGAAACAGGGCAGGTTATATCGACGTTCTCCACTGGGAAGATCCCTTATGTTGTGAAGCTTAACCCGGATGAGGATAAACAGAATGTTTTGTTGGCTGGTATGAGTGATAAGAAGATTGTTCAGTGGGATATGAATACAGGGCAGATAACTCAAGAGTATGATCAGCATTTGGGGGCTGTCAATACCATTACTTTTGTTGATAACAACAGGAGATTTGTTACTTCTAGTGATGACAAGTCCCTCAGGGTATGGGAGTATGGTATTCCCGTGGTTATAAAGTATATTAGTGAGCCACATATGCACTCTATGCCTTCCATTTCGCTTCACCCCAACGCGAATTGGCTCGCCGCGCAGAGCTTGGATAACCAGATTCTTATTTATAGCACAAGGGAGAAGTTTCAACTTAACAAAAAGAAGAGGTTTGCTGGACACATTGTTGCTGGATATGCTTGTCAGGTCAATTTTTCGCCGGATGGACGTTTTGTCATGTCGGGAGATGGTGAGGGTAAGTGTTGGTTCTGGGATTGGAAGAGTTGCAAAGTCTTTAGAACTCTCAAGTGTCACGAAGGCGTTACCATCGGTTGCCAGTGGCATCCTCTGGAACAGAGCAAAGTAGCGACGTGTGGCTGGGACGGGTTGATAAAGTACTGGTAAGTAAACTCTCATGCTGTGCCATATATAATTACAGAGTCTTATATAGTATGTTGTTCTTTGAAACATGCCTCACTGTCAGTTACTCATTGCATGCTAGATTTTCTGTTACCATTTTGATAGAATTTTGTGTATGGTTATTCAATTCAAGCTAATATAGGTCTCTAGTTCATTTAGCTCATAACACATCTCTTAACTTCTTTAGATTGCATCAAATTATAGGGTCATGATAGTCATCATTAGTTTATTGGTCCAACAGTCTAGCAGGAGACATGTGAAGGTGTTTTGA
Protein sequence

>MS.gene033400.t1

MDLLNQYSDNDPGSPTQNPDSPENSPPRLLPSRSAAPKVDDTMLTLTVADPKTLSKPIDPTQHLVAFNPTYDQLWAPIQGPSHPYAKDGLAQGMRNHKLGFVEDANIEPFLFDEQHNTFLKFGYAADPSASNYIGDFDALQKNNAASVYNIPHREQKKRKIESLKKKEENDDEDDENNDEEGVEIENPASEAWLLKNKKSPWAGKKEGLQGELTEDQKKYAEEHAKKKGEEKSGFGGEKVEAVKDKSTFHGKEERDYQGRSWIAPPKDAKASNDHCFIPKRLVHTWSGHTKGVSAIRFFPNSGHLILSAGMDTKVKIWDVFNTGKCMRTYMGHSKAVRDICFTNDGTKFLSAGYDKNIKYWDTETGQVISTFSTGKIPYVVKLNPDEDKQNVLLAGMSDKKIVQWDMNTGQITQEYDQHLGAVNTITFVDNNRRFVTSSDDKSLRVWEYGIPVVIKYISEPHMHSMPSISLHPNANWLAAQSLDNQILIYSTREKFQLNKKKRFAGHIVAGYACQVNFSPDGRFVMSGDGEGKCWFWDWKSCKVFRTLKCHEGVTIGCQWHPLEQSKVATCGWDGLIKYWVMIVIISLLVQQSSRRHVKVF