Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033949.t1 | A4ULF8.1 | 100 | 396 | 0 | 0 | 1 | 396 | 1 | 396 | 2.50E-232 | 814.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033949.t1 | A4ULF8 | 100.0 | 396 | 0 | 0 | 1 | 396 | 1 | 396 | 5.9e-235 | 814.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033949.t1 | A0A072V8Q4 | 99.5 | 396 | 2 | 0 | 1 | 396 | 1 | 396 | 2.0e-231 | 810.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene033949 | MS.gene050426 | PPI |
| MS.gene033949 | MS.gene28853 | PPI |
| MS.gene033949 | MS.gene28651 | PPI |
| MS.gene072659 | MS.gene033949 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033949.t1 | MTR_2g046710 | 99.495 | 396 | 2 | 0 | 1 | 396 | 1 | 396 | 0.0 | 816 |
| MS.gene033949.t1 | MTR_4g123810 | 93.622 | 392 | 25 | 0 | 1 | 392 | 1 | 392 | 0.0 | 741 |
| MS.gene033949.t1 | MTR_7g110310 | 93.316 | 389 | 26 | 0 | 4 | 392 | 3 | 391 | 0.0 | 739 |
| MS.gene033949.t1 | MTR_7g102120 | 90.415 | 386 | 37 | 0 | 4 | 389 | 2 | 387 | 0.0 | 704 |
| MS.gene033949.t1 | MTR_1g063060 | 87.824 | 386 | 47 | 0 | 4 | 389 | 2 | 387 | 0.0 | 693 |
| MS.gene033949.t1 | MTR_1g063060 | 87.824 | 386 | 47 | 0 | 4 | 389 | 20 | 405 | 0.0 | 692 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033949.t1 | AT3G17390 | 94.103 | 390 | 23 | 0 | 4 | 393 | 2 | 391 | 0.0 | 743 |
| MS.gene033949.t1 | AT1G02500 | 92.545 | 389 | 29 | 0 | 4 | 392 | 2 | 390 | 0.0 | 724 |
| MS.gene033949.t1 | AT1G02500 | 92.545 | 389 | 29 | 0 | 4 | 392 | 2 | 390 | 0.0 | 724 |
| MS.gene033949.t1 | AT4G01850 | 91.003 | 389 | 35 | 0 | 4 | 392 | 2 | 390 | 0.0 | 721 |
| MS.gene033949.t1 | AT4G01850 | 91.003 | 389 | 35 | 0 | 4 | 392 | 2 | 390 | 0.0 | 721 |
| MS.gene033949.t1 | AT2G36880 | 90.415 | 386 | 37 | 0 | 4 | 389 | 2 | 387 | 0.0 | 707 |
| MS.gene033949.t1 | AT2G36880 | 90.415 | 386 | 37 | 0 | 4 | 389 | 2 | 387 | 0.0 | 707 |
Find 0 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGAGTATTTCAATGACAA+GGG | + | 15213:9416-9435 | MS.gene033949:CDS | 25.0% |
| ! | TATTGAGTATTTCAATGACA+AGG | + | 15213:9415-9434 | MS.gene033949:CDS | 25.0% |
| AGGTTTCTTTCACAATGTTA+AGG | - | 15213:9898-9917 | MS.gene033949:intergenic | 30.0% | |
| ATGTTAAGGATTTCCTTATC+AGG | - | 15213:9884-9903 | MS.gene033949:intergenic | 30.0% | |
| GAAATACTCAATAGTGACTT+GGG | - | 15213:9408-9427 | MS.gene033949:intergenic | 30.0% | |
| TATCAACCTTGATCTTAAGA+GGG | + | 15213:9943-9962 | MS.gene033949:CDS | 30.0% | |
| TGAAATACTCAATAGTGACT+TGG | - | 15213:9409-9428 | MS.gene033949:intergenic | 30.0% | |
| TGTTAAGGATTTCCTTATCA+GGG | - | 15213:9883-9902 | MS.gene033949:intergenic | 30.0% | |
| AAGATCATCATCGATACCTA+CGG | + | 15213:9653-9672 | MS.gene033949:CDS | 35.0% | |
| ACAAAAAGACCTGAGGAAAT+TGG | + | 15213:9230-9249 | MS.gene033949:CDS | 35.0% | |
| ACCGTAAGAATCAACAAACA+CGG | - | 15213:9849-9868 | MS.gene033949:intergenic | 35.0% | |
| ATCAACCTTGATCTTAAGAG+GGG | + | 15213:9944-9963 | MS.gene033949:CDS | 35.0% | |
| ATCAGCTGCAATTTCATCAT+TGG | - | 15213:9504-9523 | MS.gene033949:intergenic | 35.0% | |
| CTATCAACCTTGATCTTAAG+AGG | + | 15213:9942-9961 | MS.gene033949:CDS | 35.0% | |
| TAACTTGGTTGCAAGAACAT+GGG | - | 15213:9321-9340 | MS.gene033949:intergenic | 35.0% | |
| TCACAGATTCAGATGTGTAT+AGG | - | 15213:8911-8930 | MS.gene033949:intergenic | 35.0% | |
| TCTGTTGTTCAATGTTGACA+AGG | - | 15213:9175-9194 | MS.gene033949:intergenic | 35.0% | |
| ! | GACTTTTGTTGGATCTTTAC+CGG | - | 15213:9711-9730 | MS.gene033949:intergenic | 35.0% |
| ! | TGAAAGAAACCTTTGACTTC+AGG | + | 15213:9906-9925 | MS.gene033949:CDS | 35.0% |
| !! | AGATGATGCTGATTTCACAT+GGG | + | 15213:10015-10034 | MS.gene033949:CDS | 35.0% |
| !! | GCAAAGAGTATTGTTGCTAA+TGG | + | 15213:9764-9783 | MS.gene033949:CDS | 35.0% |
| !! | TCCGTGTTTGTTGATTCTTA+CGG | + | 15213:9845-9864 | MS.gene033949:CDS | 35.0% |
| !! | TTTGTTGATTCTTACGGTAC+CGG | + | 15213:9851-9870 | MS.gene033949:CDS | 35.0% |
| !! | TTTTTGAAGACTGCTGCTTA+TGG | + | 15213:9980-9999 | MS.gene033949:CDS | 35.0% |
| !!! | GGTTTTGTTTCAGATGATGT+TGG | + | 15213:9125-9144 | MS.gene033949:CDS | 35.0% |
| AACTTGCACAAAGACCAACA+TGG | + | 15213:9025-9044 | MS.gene033949:CDS | 40.0% | |
| AAGATCCAACAAAAGTCGAC+AGG | + | 15213:9714-9733 | MS.gene033949:CDS | 40.0% | |
| ACATCTGAATCTGTGAACGA+GGG | + | 15213:8915-8934 | MS.gene033949:CDS | 40.0% | |
| ACCAAAGACCATAACCATGT+TGG | - | 15213:9042-9061 | MS.gene033949:intergenic | 40.0% | |
| ACCAACATGGTTATGGTCTT+TGG | + | 15213:9038-9057 | MS.gene033949:CDS | 40.0% | |
| AGATTGTTCGTAACACATGC+AGG | + | 15213:9096-9115 | MS.gene033949:CDS | 40.0% | |
| CACAAAGACCAACATGGTTA+TGG | + | 15213:9031-9050 | MS.gene033949:CDS | 40.0% | |
| CATAAGCTCAGGTGTTTCAT+CGG | - | 15213:9291-9310 | MS.gene033949:intergenic | 40.0% | |
| CATGTTCTTGCAACCAAGTT+AGG | + | 15213:9320-9339 | MS.gene033949:CDS | 40.0% | |
| CCATCTTACAAAAAGACCTG+AGG | + | 15213:9223-9242 | MS.gene033949:CDS | 40.0% | |
| CTAACTTGGTTGCAAGAACA+TGG | - | 15213:9322-9341 | MS.gene033949:intergenic | 40.0% | |
| GAATCAACAAACACGGACAA+TGG | - | 15213:9842-9861 | MS.gene033949:intergenic | 40.0% | |
| GTGCAAGTTTCCTATGCTAT+CGG | + | 15213:9806-9825 | MS.gene033949:CDS | 40.0% | |
| TGAACACCTTGAGCAATATC+AGG | - | 15213:9200-9219 | MS.gene033949:intergenic | 40.0% | |
| TTAAGAGGGGTGGAAATAAC+AGG | + | 15213:9957-9976 | MS.gene033949:CDS | 40.0% | |
| TTCCTTATCAGGGATCTTTC+CGG | - | 15213:9873-9892 | MS.gene033949:intergenic | 40.0% | |
| ! | CCTCAGGTCTTTTTGTAAGA+TGG | - | 15213:9226-9245 | MS.gene033949:intergenic | 40.0% |
| ! | GATATTGCTCAAGGTGTTCA+TGG | + | 15213:9200-9219 | MS.gene033949:CDS | 40.0% |
| ! | GGAAGATGGTTTTCTCATCA+AGG | - | 15213:9568-9587 | MS.gene033949:intergenic | 40.0% |
| ! | GTTGCAAGAACATGGGTTAA+AGG | - | 15213:9314-9333 | MS.gene033949:intergenic | 40.0% |
| ! | TCTCATCAAGGTACTTCTCT+GGG | - | 15213:9556-9575 | MS.gene033949:intergenic | 40.0% |
| ! | TTCTCATCAAGGTACTTCTC+TGG | - | 15213:9557-9576 | MS.gene033949:intergenic | 40.0% |
| !! | ACTGCTGCTTATGGACATTT+TGG | + | 15213:9989-10008 | MS.gene033949:CDS | 40.0% |
| !! | AGGTATCGATGATGATCTTG+CGG | - | 15213:9652-9671 | MS.gene033949:intergenic | 40.0% |
| !! | AGGTTGATAGAGATCATTCC+TGG | - | 15213:9932-9951 | MS.gene033949:intergenic | 40.0% |
| !! | GAGATGATGCTGATTTCACA+TGG | + | 15213:10014-10033 | MS.gene033949:CDS | 40.0% |
| !! | TTGCTAATGGACTTGCTAGA+AGG | + | 15213:9777-9796 | MS.gene033949:CDS | 40.0% |
| AACCTTGATCTTAAGAGGGG+TGG | + | 15213:9947-9966 | MS.gene033949:CDS | 45.0% | |
| AGATAAGGACGGTATGGACA+CGG | - | 15213:9457-9476 | MS.gene033949:intergenic | 45.0% | |
| AGTGAGCTTAGCTCCTAACT+TGG | - | 15213:9336-9355 | MS.gene033949:intergenic | 45.0% | |
| ATCATCATCGATACCTACGG+TGG | + | 15213:9656-9675 | MS.gene033949:CDS | 45.0% | |
| ATCTTCCACTTGAACCCTTC+AGG | + | 15213:9581-9600 | MS.gene033949:CDS | 45.0% | |
| CACATCTGAATCTGTGAACG+AGG | + | 15213:8914-8933 | MS.gene033949:CDS | 45.0% | |
| CAGAGTCCTGATATTGCTCA+AGG | + | 15213:9191-9210 | MS.gene033949:CDS | 45.0% | |
| CGTAACACATGCAGGAACAT+AGG | + | 15213:9104-9123 | MS.gene033949:CDS | 45.0% | |
| CTCAAGGCAAGCATCAAGAA+CGG | - | 15213:8976-8995 | MS.gene033949:intergenic | 45.0% | |
| CTCACTGAAGTTCGCAAGAA+CGG | + | 15213:9350-9369 | MS.gene033949:CDS | 45.0% | |
| GAAGTTGTGAAGCCACTCAA+GGG | + | 15213:10037-10056 | MS.gene033949:CDS | 45.0% | |
| GGTGATCAAGGTCACATGTT+CGG | + | 15213:9257-9276 | MS.gene033949:CDS | 45.0% | |
| GTGATCAAGGTCACATGTTC+GGG | + | 15213:9258-9277 | MS.gene033949:CDS | 45.0% | |
| GTGCTGTGTTGAGATAAGGA+CGG | - | 15213:9468-9487 | MS.gene033949:intergenic | 45.0% | |
| GTGTTGAGATAAGGACGGTA+TGG | - | 15213:9463-9482 | MS.gene033949:intergenic | 45.0% | |
| TACCGGAAAGATCCCTGATA+AGG | + | 15213:9868-9887 | MS.gene033949:CDS | 45.0% | |
| TGAAGGGTTCAAGTGGAAGA+TGG | - | 15213:9582-9601 | MS.gene033949:intergenic | 45.0% | |
| TTCCACCCCTCTTAAGATCA+AGG | - | 15213:9952-9971 | MS.gene033949:intergenic | 45.0% | |
| TTTCCATCAGGTCTCAACCA+AGG | - | 15213:9383-9402 | MS.gene033949:intergenic | 45.0% | |
| ! | TCACATGCAACTTTGCTGTC+TGG | - | 15213:9005-9024 | MS.gene033949:intergenic | 45.0% |
| ! | TGGGTTAAAGGCATAAGCTC+AGG | - | 15213:9302-9321 | MS.gene033949:intergenic | 45.0% |
| !! | GAAATTGGTGCTGGTGATCA+AGG | + | 15213:9245-9264 | MS.gene033949:CDS | 45.0% |
| !! | TTTCAATGACAAGGGTGCCA+TGG | + | 15213:9424-9443 | MS.gene033949:CDS | 45.0% |
| ACAAACACGGACAATGGCTC+TGG | - | 15213:9836-9855 | MS.gene033949:intergenic | 50.0% | |
| AGACCTGAGGAAATTGGTGC+TGG | + | 15213:9236-9255 | MS.gene033949:CDS | 50.0% | |
| ATCGATACCTACGGTGGTTG+GGG | + | 15213:9662-9681 | MS.gene033949:CDS | 50.0% | |
| ATCGATGATGATCTTGCGGC+CGG | - | 15213:9648-9667 | MS.gene033949:intergenic | 50.0% | |
| ATCTGGTCGCAAAGCTTGTC+GGG | - | 15213:8945-8964 | MS.gene033949:intergenic | 50.0% | |
| CAAGAACGGCATCAGAGATC+TGG | - | 15213:8962-8981 | MS.gene033949:intergenic | 50.0% | |
| CATCGATACCTACGGTGGTT+GGG | + | 15213:9661-9680 | MS.gene033949:CDS | 50.0% | |
| CATTCCTGGCCTGAAGTCAA+AGG | - | 15213:9918-9937 | MS.gene033949:intergenic | 50.0% | |
| CCAACAAAAGTCGACAGGAG+TGG | + | 15213:9719-9738 | MS.gene033949:CDS | 50.0% | |
| CGTCGTGCTGTGTTGAGATA+AGG | - | 15213:9472-9491 | MS.gene033949:intergenic | 50.0% | |
| GATCTGGTCGCAAAGCTTGT+CGG | - | 15213:8946-8965 | MS.gene033949:intergenic | 50.0% | |
| GCAAGAACGGAACATGTCCT+TGG | + | 15213:9363-9382 | MS.gene033949:CDS | 50.0% | |
| GCTAACTTTCCACCCTTGAG+TGG | - | 15213:10052-10071 | MS.gene033949:intergenic | 50.0% | |
| GGAAGTTGTGAAGCCACTCA+AGG | + | 15213:10036-10055 | MS.gene033949:CDS | 50.0% | |
| TCACCAGCACCAATTTCCTC+AGG | - | 15213:9242-9261 | MS.gene033949:intergenic | 50.0% | |
| TCATCGATACCTACGGTGGT+TGG | + | 15213:9660-9679 | MS.gene033949:CDS | 50.0% | |
| TGCTGTCTGGATCTTGCTCA+AGG | - | 15213:8992-9011 | MS.gene033949:intergenic | 50.0% | |
| TGTCCTTGGTTGAGACCTGA+TGG | + | 15213:9377-9396 | MS.gene033949:CDS | 50.0% | |
| ! | GAAACCTTTGACTTCAGGCC+AGG | + | 15213:9911-9930 | MS.gene033949:CDS | 50.0% |
| ! | GTGACTTGGGTCTTTCCATC+AGG | - | 15213:9395-9414 | MS.gene033949:intergenic | 50.0% |
| !! | AGGTACTTCTCTGGGATCAC+AGG | - | 15213:9548-9567 | MS.gene033949:intergenic | 50.0% |
| !! | CTCTGGAACACCGATAGCAT+AGG | - | 15213:9819-9838 | MS.gene033949:intergenic | 50.0% |
| !!! | CCACTCCTGTCGACTTTTGT+TGG | - | 15213:9722-9741 | MS.gene033949:intergenic | 50.0% |
| AACGGCCTGAAGGGTTCAAG+TGG | - | 15213:9589-9608 | MS.gene033949:intergenic | 55.0% | |
| AGGACGGTATGGACACGGAT+CGG | - | 15213:9452-9471 | MS.gene033949:intergenic | 55.0% | |
| ATGGACACGGATCGGAACCA+TGG | - | 15213:9444-9463 | MS.gene033949:intergenic | 55.0% | |
| CCTTCAGGCCGTTTCGTCAT+CGG | + | 15213:9596-9615 | MS.gene033949:CDS | 55.0% | |
| CGATGACGAAACGGCCTGAA+GGG | - | 15213:9598-9617 | MS.gene033949:intergenic | 55.0% | |
| GAGGACCACCGATGACGAAA+CGG | - | 15213:9607-9626 | MS.gene033949:intergenic | 55.0% | |
| GTTGTGAAGCCACTCAAGGG+TGG | + | 15213:10040-10059 | MS.gene033949:CDS | 55.0% | |
| TCTGGTCGCAAAGCTTGTCG+GGG | - | 15213:8944-8963 | MS.gene033949:intergenic | 55.0% | |
| TTCGTCATCGGTGGTCCTCA+CGG | + | 15213:9608-9627 | MS.gene033949:CDS | 55.0% | |
| CCGATGACGAAACGGCCTGA+AGG | - | 15213:9599-9618 | MS.gene033949:intergenic | 60.0% | |
| GTTAGACCAGCGTCACCGTG+AGG | - | 15213:9626-9645 | MS.gene033949:intergenic | 60.0% | |
| TACGGTGGTTGGGGTGCACA+CGG | + | 15213:9671-9690 | MS.gene033949:CDS | 60.0% | |
| TCAGGCCGTTTCGTCATCGG+TGG | + | 15213:9599-9618 | MS.gene033949:CDS | 60.0% | |
| ! | CACGGTGACGCTGGTCTAAC+CGG | + | 15213:9626-9645 | MS.gene033949:CDS | 60.0% |
| ! | CACGGTGGTGGTGCTTTCTC+CGG | + | 15213:9689-9708 | MS.gene033949:CDS | 60.0% |
| GTGTGCACCCCAACCACCGT+AGG | - | 15213:9672-9691 | MS.gene033949:intergenic | 65.0% | |
| GGTGGTCCTCACGGTGACGC+TGG | + | 15213:9617-9636 | MS.gene033949:CDS | 70.0% | |
| GGTGGTTGGGGTGCACACGG+TGG | + | 15213:9674-9693 | MS.gene033949:CDS | 70.0% | |
| GGTTGGGGTGCACACGGTGG+TGG | + | 15213:9677-9696 | MS.gene033949:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15213 | gene | 8891 | 10081 | 8891 | ID=MS.gene033949 |
| 15213 | mRNA | 8891 | 10081 | 8891 | ID=MS.gene033949.t1;Parent=MS.gene033949 |
| 15213 | exon | 8891 | 10081 | 8891 | ID=MS.gene033949.t1.exon1;Parent=MS.gene033949.t1 |
| 15213 | CDS | 8891 | 10081 | 8891 | ID=cds.MS.gene033949.t1;Parent=MS.gene033949.t1 |
| Gene Sequence |
| Protein sequence |
