Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034818.t1 | XP_013467919.1 | 100 | 146 | 0 | 0 | 1 | 146 | 1 | 146 | 3.00E-82 | 314.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034818.t1 | Q9SVD7 | 92.5 | 146 | 11 | 0 | 1 | 146 | 1 | 146 | 2.6e-79 | 295.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034818.t1 | B7FNB3 | 100.0 | 146 | 0 | 0 | 1 | 146 | 1 | 146 | 2.1e-82 | 314.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene034818 | MS.gene020503 | PPI |
| MS.gene034818 | MS.gene015674 | PPI |
| MS.gene29973 | MS.gene034818 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034818.t1 | MTR_1g058100 | 100.000 | 146 | 0 | 0 | 1 | 146 | 1 | 146 | 1.11e-109 | 308 |
| MS.gene034818.t1 | MTR_1g058100 | 98.649 | 148 | 0 | 1 | 1 | 146 | 1 | 148 | 6.82e-108 | 303 |
| MS.gene034818.t1 | MTR_1g058100 | 87.671 | 146 | 0 | 1 | 1 | 146 | 1 | 128 | 3.64e-90 | 258 |
| MS.gene034818.t1 | MTR_8g105010 | 80.142 | 141 | 28 | 0 | 3 | 143 | 1 | 141 | 5.09e-84 | 243 |
| MS.gene034818.t1 | MTR_8g105010 | 80.000 | 140 | 28 | 0 | 3 | 142 | 1 | 140 | 6.86e-83 | 241 |
| MS.gene034818.t1 | MTR_1g058100 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 6.96e-73 | 214 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034818.t1 | AT3G52560 | 92.466 | 146 | 11 | 0 | 1 | 146 | 1 | 146 | 2.04e-102 | 289 |
| MS.gene034818.t1 | AT2G36060 | 93.151 | 146 | 9 | 1 | 1 | 146 | 1 | 145 | 6.19e-102 | 288 |
| MS.gene034818.t1 | AT3G52560 | 91.837 | 147 | 11 | 1 | 1 | 146 | 1 | 147 | 1.04e-100 | 285 |
| MS.gene034818.t1 | AT2G36060 | 92.517 | 147 | 9 | 2 | 1 | 146 | 1 | 146 | 3.26e-100 | 284 |
| MS.gene034818.t1 | AT2G36060 | 92.466 | 146 | 9 | 2 | 1 | 146 | 1 | 144 | 1.80e-99 | 282 |
| MS.gene034818.t1 | AT3G52560 | 82.317 | 164 | 11 | 1 | 1 | 146 | 1 | 164 | 3.61e-98 | 279 |
| MS.gene034818.t1 | AT1G23260 | 75.177 | 141 | 35 | 0 | 3 | 143 | 1 | 141 | 6.55e-80 | 233 |
| MS.gene034818.t1 | AT1G70660 | 73.239 | 142 | 38 | 0 | 3 | 144 | 1 | 142 | 2.71e-78 | 228 |
| MS.gene034818.t1 | AT1G70660 | 73.239 | 142 | 38 | 0 | 3 | 144 | 1 | 142 | 4.98e-78 | 228 |
| MS.gene034818.t1 | AT3G52560 | 90.000 | 110 | 10 | 1 | 38 | 146 | 1 | 110 | 2.16e-73 | 215 |
| MS.gene034818.t1 | AT1G70650 | 73.529 | 136 | 36 | 0 | 8 | 143 | 442 | 577 | 3.88e-71 | 224 |
| MS.gene034818.t1 | AT1G23260 | 72.642 | 106 | 29 | 0 | 38 | 143 | 1 | 106 | 1.19e-57 | 176 |
Find 0 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAGAAAAATGGTAAAA+TGG | - | 8685:234181-234200 | MS.gene034818:intron | 15.0% |
| !!! | ATGTAATTGTTGTTAATTTT+GGG | - | 8685:233672-233691 | MS.gene034818:intron | 15.0% |
| !!! | TATGTAATTGTTGTTAATTT+TGG | - | 8685:233671-233690 | MS.gene034818:intron | 15.0% |
| !!! | TGATACTTTAAAAAGAAAAA+TGG | - | 8685:234173-234192 | MS.gene034818:intron | 15.0% |
| !!! | TTTTTTTTTTATTTTACCGA+TGG | - | 8685:233708-233727 | MS.gene034818:intron | 15.0% |
| !! | AATCAAGATTTATATGAAAC+AGG | - | 8685:235540-235559 | MS.gene034818:intron | 20.0% |
| !! | ATATGAAATATTGTTGTATC+TGG | - | 8685:235124-235143 | MS.gene034818:intron | 20.0% |
| !! | CTGTAATTGAATTATAATTC+TGG | - | 8685:234237-234256 | MS.gene034818:intron | 20.0% |
| ! | AAAAATGAAAACGACGAAAA+CGG | + | 8685:233537-233556 | None:intergenic | 25.0% |
| ! | AAAGAAAAATGGTAAAATGG+TGG | - | 8685:234184-234203 | MS.gene034818:intron | 25.0% |
| ! | AAGAAAAATGGTAAAATGGT+GGG | - | 8685:234185-234204 | MS.gene034818:intron | 25.0% |
| ! | ACAGAGAAATACTTACATTA+TGG | + | 8685:234621-234640 | None:intergenic | 25.0% |
| ! | AGAGAAATACTTACATTATG+GGG | + | 8685:234619-234638 | None:intergenic | 25.0% |
| ! | CAGAGAAATACTTACATTAT+GGG | + | 8685:234620-234639 | None:intergenic | 25.0% |
| ! | GATATGTACAAAATATTAGC+CGG | + | 8685:234275-234294 | None:intergenic | 25.0% |
| !! | AAAACTACATCGATGATTTT+AGG | + | 8685:233640-233659 | None:intergenic | 25.0% |
| !! | ATTGAAATGTTCGATCATTT+TGG | - | 8685:233839-233858 | MS.gene034818:CDS | 25.0% |
| !!! | TAAACTGGTTCTATCAAATT+AGG | + | 8685:234735-234754 | None:intergenic | 25.0% |
| !!! | TTATTTTACCGATGGAATTT+TGG | - | 8685:233716-233735 | MS.gene034818:intron | 25.0% |
| !!! | TTTTTAGTAGTTTCTTGTTG+AGG | - | 8685:233921-233940 | MS.gene034818:intron | 25.0% |
| AGGTTGAACCAAAGAAATTT+GGG | - | 8685:235560-235579 | MS.gene034818:intron | 30.0% | |
| ATATTACGAACACCTAACAA+TGG | + | 8685:234657-234676 | None:intergenic | 30.0% | |
| ATTACAGAAAACTTGCACAA+AGG | + | 8685:234224-234243 | None:intergenic | 30.0% | |
| CATGAACACTTCTTACTTAT+TGG | - | 8685:235017-235036 | MS.gene034818:intron | 30.0% | |
| GAGAAATACTTACATTATGG+GGG | + | 8685:234618-234637 | None:intergenic | 30.0% | |
| GATTACATCTCTGATAACAA+TGG | + | 8685:235524-235543 | None:intergenic | 30.0% | |
| TATCTGGAAATCTGAAAGTT+CGG | - | 8685:235140-235159 | MS.gene034818:intron | 30.0% | |
| TTTGATAGAACCAGTTTATG+TGG | - | 8685:234737-234756 | MS.gene034818:intron | 30.0% | |
| ! | CGCATAAACTACAATTTTCA+AGG | + | 8685:234828-234847 | None:intergenic | 30.0% |
| AATTTGGGCTTCTTGCAAAT+TGG | - | 8685:235575-235594 | MS.gene034818:intron | 35.0% | |
| ACTTGTGTTAACCATGAAAC+TGG | - | 8685:235364-235383 | MS.gene034818:intron | 35.0% | |
| ATGAATGTTACTGCATGTGT+CGG | - | 8685:234031-234050 | MS.gene034818:intron | 35.0% | |
| CAAGAACTCAAAAAACATGC+CGG | + | 8685:234862-234881 | None:intergenic | 35.0% | |
| CATATCATGTGTTTATCCTG+AGG | - | 8685:234289-234308 | MS.gene034818:intron | 35.0% | |
| TAAAAGTAAGTTCCTTCTGG+AGG | + | 8685:235682-235701 | None:intergenic | 35.0% | |
| TACTTTCAGACTGTACATGA+AGG | - | 8685:235256-235275 | MS.gene034818:intron | 35.0% | |
| TATCCAACTTCCACATAAAC+TGG | + | 8685:234750-234769 | None:intergenic | 35.0% | |
| TGATGTCTTGTGACTGTAAA+GGG | - | 8685:234338-234357 | MS.gene034818:intron | 35.0% | |
| TTGATGTCTTGTGACTGTAA+AGG | - | 8685:234337-234356 | MS.gene034818:intron | 35.0% | |
| TTTCAAATATGTACTGCCTC+AGG | + | 8685:234308-234327 | None:intergenic | 35.0% | |
| ! | ATTCTGGTTGTTCTTGTTTC+CGG | - | 8685:234253-234272 | MS.gene034818:intron | 35.0% |
| ! | CAGGTTGAACCAAAGAAATT+TGG | - | 8685:235559-235578 | MS.gene034818:intron | 35.0% |
| ! | CTCTGATAACAATGGATTCA+AGG | + | 8685:235516-235535 | None:intergenic | 35.0% |
| ! | GAAGATGATATGAAAGCATG+TGG | + | 8685:235410-235429 | None:intergenic | 35.0% |
| ! | TCATTTTGTGCGTACTTAAC+AGG | - | 8685:235197-235216 | MS.gene034818:intron | 35.0% |
| ! | TTACCGATGGAATTTTGGAT+CGG | - | 8685:233721-233740 | MS.gene034818:intron | 35.0% |
| ! | TTCGAGCTGTGAATTTTGTT+CGG | - | 8685:233754-233773 | MS.gene034818:intron | 35.0% |
| ! | TTTATGCGAAGTGTTTACAC+CGG | - | 8685:234840-234859 | MS.gene034818:intron | 35.0% |
| !! | AACACAATCTCTCTGCTTTT+CGG | - | 8685:235484-235503 | MS.gene034818:intron | 35.0% |
| !! | CATCGATGATTTTAGGTCAA+CGG | + | 8685:233633-233652 | None:intergenic | 35.0% |
| AAAACGGCGATGAGTTAGAT+TGG | + | 8685:233521-233540 | None:intergenic | 40.0% | |
| ACTTCTCTGCATCCATTGTT+AGG | - | 8685:234642-234661 | MS.gene034818:CDS | 40.0% | |
| ATGACATTTACATGCGCTCT+TGG | - | 8685:234575-234594 | MS.gene034818:CDS | 40.0% | |
| CTGTATTTGCACATGAACAC+TGG | - | 8685:234925-234944 | MS.gene034818:intron | 40.0% | |
| GAACCAGTTTATGTGGAAGT+TGG | - | 8685:234744-234763 | MS.gene034818:intron | 40.0% | |
| GCAAGAAGCCCAAATTTCTT+TGG | + | 8685:235571-235590 | None:intergenic | 40.0% | |
| GTTAGCTATGGAATGGATGA+TGG | - | 8685:234550-234569 | MS.gene034818:intron | 40.0% | |
| TATCCGTACTTCAGAGTTTC+CGG | - | 8685:234120-234139 | MS.gene034818:intron | 40.0% | |
| TCACCGATCCAAAATTCCAT+CGG | + | 8685:233727-233746 | None:intergenic | 40.0% | |
| ! | GAACGAGGTGAAAAAGGTAT+TGG | - | 8685:234517-234536 | MS.gene034818:intron | 40.0% |
| ! | GTACTTAACAGGTGGTTTTC+AGG | - | 8685:235208-235227 | MS.gene034818:intron | 40.0% |
| ! | TATGTTCTGTTGCTTCAAGC+TGG | + | 8685:234790-234809 | None:intergenic | 40.0% |
| ! | TGCCATTTCTTTCTTCAGCT+GGG | + | 8685:235632-235651 | None:intergenic | 40.0% |
| ! | TTTTGTGCGTACTTAACAGG+TGG | - | 8685:235200-235219 | MS.gene034818:intron | 40.0% |
| !! | GGTGAAAAAGGTATTGGTGA+TGG | - | 8685:234523-234542 | MS.gene034818:intron | 40.0% |
| !! | GTGTAGTTGGTGTTAGGATT+CGG | - | 8685:234053-234072 | MS.gene034818:intron | 40.0% |
| AAGGAACTTCAGATTGCTGG+AGG | - | 8685:234489-234508 | MS.gene034818:intron | 45.0% | |
| ATGCGTGTCAGACACTTTGA+CGG | - | 8685:234082-234101 | MS.gene034818:intron | 45.0% | |
| ATTTACATGCGCTCTTGGAC+TGG | - | 8685:234580-234599 | MS.gene034818:CDS | 45.0% | |
| CATATGCCCTTCCTATGTTC+TGG | - | 8685:235060-235079 | MS.gene034818:intron | 45.0% | |
| CATCACACTTATCTCGTAGC+CGG | + | 8685:234142-234161 | None:intergenic | 45.0% | |
| CGTGAATGAAACCGAACACT+TGG | + | 8685:235334-235353 | None:intergenic | 45.0% | |
| GAATGAAACCGAACACTTGG+CGG | + | 8685:235331-235350 | None:intergenic | 45.0% | |
| GACCCAGCTGAAGAAAGAAA+TGG | - | 8685:235627-235646 | MS.gene034818:intron | 45.0% | |
| GAGCTTGAACGAGGTGAAAA+AGG | - | 8685:234511-234530 | MS.gene034818:intron | 45.0% | |
| GCTACGAGATAAGTGTGATG+AGG | - | 8685:234142-234161 | MS.gene034818:intron | 45.0% | |
| GCTCACAAGCAACTAAACAC+AGG | + | 8685:234897-234916 | None:intergenic | 45.0% | |
| TACATTATGGGGGCCAATGA+TGG | + | 8685:234608-234627 | None:intergenic | 45.0% | |
| TCCAAGGAACTTCAGATTGC+TGG | - | 8685:234486-234505 | MS.gene034818:intron | 45.0% | |
| TGGCACTGTTAGCTATGGAA+TGG | - | 8685:234543-234562 | MS.gene034818:intron | 45.0% | |
| TGTTAACCATGAAACTGGCG+TGG | - | 8685:235369-235388 | MS.gene034818:intron | 45.0% | |
| ! | CTGCCATTTCTTTCTTCAGC+TGG | + | 8685:235633-235652 | None:intergenic | 45.0% |
| ! | TCCAGCAATCTGAAGTTCCT+TGG | + | 8685:234490-234509 | None:intergenic | 45.0% |
| ! | TTGGCAAAGAGAGTACAGCA+TGG | - | 8685:235594-235613 | MS.gene034818:intron | 45.0% |
| !! | TAGCCGGAAACTCTGAAGTA+CGG | + | 8685:234126-234145 | None:intergenic | 45.0% |
| AAATGGCAGCCCCTCATAAC+CGG | - | 8685:235644-235663 | MS.gene034818:intron | 50.0% | |
| ATGCCCTTCCTATGTTCTGG+CGG | - | 8685:235063-235082 | MS.gene034818:intron | 50.0% | |
| CAGAAAAGCCGCCAAGTGTT+CGG | - | 8685:235320-235339 | MS.gene034818:intron | 50.0% | |
| CTGATACCACGCCAGTTTCA+TGG | + | 8685:235378-235397 | None:intergenic | 50.0% | |
| GAAAAGACCCGCCAGAACAT+AGG | + | 8685:235074-235093 | None:intergenic | 50.0% | |
| GACAAGCTTCCGGTTATGAG+GGG | + | 8685:235656-235675 | None:intergenic | 50.0% | |
| GGACAAGCTTCCGGTTATGA+GGG | + | 8685:235657-235676 | None:intergenic | 50.0% | |
| TGCGCTTCAATGCAGTTCCA+AGG | - | 8685:234470-234489 | MS.gene034818:intron | 50.0% | |
| TGGACAAGCTTCCGGTTATG+AGG | + | 8685:235658-235677 | None:intergenic | 50.0% | |
| TGGTAACTAACCGACGACAC+TGG | + | 8685:233501-233520 | None:intergenic | 50.0% | |
| ! | ACTGCATGTGTCGGTGTAGT+TGG | - | 8685:234040-234059 | MS.gene034818:intron | 50.0% |
| ! | AGTAAGTTCCTTCTGGAGGC+TGG | + | 8685:235678-235697 | None:intergenic | 50.0% |
| ! | GAACACTTGGCGGCTTTTCT+GGG | + | 8685:235321-235340 | None:intergenic | 50.0% |
| ! | GCAAAGAGAGTACAGCATGG+AGG | - | 8685:235597-235616 | MS.gene034818:intron | 50.0% |
| ! | GGTGATGGCACTGTTAGCTA+TGG | - | 8685:234538-234557 | MS.gene034818:intron | 50.0% |
| ! | GTGTCGGTGTAGTTGGTGTT+AGG | - | 8685:234047-234066 | MS.gene034818:intron | 50.0% |
| !! | TCTTGGACTGGCACCATCAT+TGG | - | 8685:234592-234611 | MS.gene034818:CDS | 50.0% |
| AAGCTTGTCCAGCCTCCAGA+AGG | - | 8685:235667-235686 | MS.gene034818:intron | 55.0% | |
| AGACCCGCCAGAACATAGGA+AGG | + | 8685:235070-235089 | None:intergenic | 55.0% | |
| CTAACCGACGACACTGGATC+CGG | + | 8685:233495-233514 | None:intergenic | 55.0% | |
| GACCCGCCAGAACATAGGAA+GGG | + | 8685:235069-235088 | None:intergenic | 55.0% | |
| TGCCCTTCCTATGTTCTGGC+GGG | - | 8685:235064-235083 | MS.gene034818:intron | 55.0% | |
| TTGCTGGAGGAGCTTGAACG+AGG | - | 8685:234502-234521 | MS.gene034818:intron | 55.0% | |
| ! | CGAACACTTGGCGGCTTTTC+TGG | + | 8685:235322-235341 | None:intergenic | 55.0% |
| CTGGAGGCTGGACAAGCTTC+CGG | + | 8685:235666-235685 | None:intergenic | 60.0% | |
| TCAGCCGGATCCAGTGTCGT+CGG | - | 8685:233488-233507 | MS.gene034818:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8685 | gene | 233476 | 235702 | 233476 | ID=MS.gene034818 |
| 8685 | mRNA | 233476 | 235702 | 233476 | ID=MS.gene034818.t1;Parent=MS.gene034818 |
| 8685 | exon | 235669 | 235702 | 235669 | ID=MS.gene034818.t1.exon1;Parent=MS.gene034818.t1 |
| 8685 | CDS | 235669 | 235702 | 235669 | ID=cds.MS.gene034818.t1;Parent=MS.gene034818.t1 |
| 8685 | exon | 234554 | 234693 | 234554 | ID=MS.gene034818.t1.exon2;Parent=MS.gene034818.t1 |
| 8685 | CDS | 234554 | 234693 | 234554 | ID=cds.MS.gene034818.t1;Parent=MS.gene034818.t1 |
| 8685 | exon | 233788 | 233913 | 233788 | ID=MS.gene034818.t1.exon3;Parent=MS.gene034818.t1 |
| 8685 | CDS | 233788 | 233913 | 233788 | ID=cds.MS.gene034818.t1;Parent=MS.gene034818.t1 |
| 8685 | exon | 233476 | 233616 | 233476 | ID=MS.gene034818.t1.exon4;Parent=MS.gene034818.t1 |
| 8685 | CDS | 233476 | 233616 | 233476 | ID=cds.MS.gene034818.t1;Parent=MS.gene034818.t1 |
| Gene Sequence |
| Protein sequence |
