AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035022

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035022.t1 MTR_1g063060 100.000 390 0 0 1 390 19 408 0.0 805
MS.gene035022.t1 MTR_1g063060 100.000 390 0 0 1 390 1 390 0.0 805
MS.gene035022.t1 MTR_7g102120 94.103 390 23 0 1 390 1 390 0.0 740
MS.gene035022.t1 MTR_4g123810 88.601 386 44 0 2 387 4 389 0.0 696
MS.gene035022.t1 MTR_7g110310 86.788 386 51 0 2 387 3 388 0.0 693
MS.gene035022.t1 MTR_2g046710 88.083 386 46 0 2 387 4 389 0.0 692
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035022.t1 AT2G36880 92.564 390 29 0 1 390 1 390 0.0 733
MS.gene035022.t1 AT2G36880 92.564 390 29 0 1 390 1 390 0.0 733
MS.gene035022.t1 AT3G17390 88.114 387 46 0 1 387 1 387 0.0 696
MS.gene035022.t1 AT4G01850 87.597 387 48 0 1 387 1 387 0.0 692
MS.gene035022.t1 AT4G01850 87.597 387 48 0 1 387 1 387 0.0 692
MS.gene035022.t1 AT1G02500 87.597 387 48 0 1 387 1 387 0.0 688
MS.gene035022.t1 AT1G02500 87.597 387 48 0 1 387 1 387 0.0 688

Find 116 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCCAGATAAGGATATTT+TGG 0.191529 1.3:-45520001 MS.gene035022:CDS
ACTGCTGCTTACGGGCATTT+TGG 0.206540 1.3:-45519889 MS.gene035022:CDS
AGCAAGAAGCCTGAGGAAAT+TGG 0.257775 1.3:-45520648 MS.gene035022:CDS
ACTAACATGGTTATGGTCTT+TGG 0.278992 1.3:-45520840 MS.gene035022:CDS
TGAACTCCTTGAGCAATATC+AGG 0.302469 1.3:+45520681 None:intergenic
GCCCGTAAGCAGCAGTCTTC+TGG 0.322457 1.3:+45519896 None:intergenic
TCACAAGAGGTGGCAAATTC+AGG 0.332737 1.3:-45519921 MS.gene035022:CDS
AGAACCAAAATATCCTTATC+TGG 0.337009 1.3:+45519997 None:intergenic
AAATAGTTCGTGACACTTGC+AGG 0.350940 1.3:-45520782 MS.gene035022:CDS
AGGATTGTGTGCACGCGAAT+AGG 0.355191 1.3:+45520429 None:intergenic
TATTGATACCTATGGTGGTT+GGG 0.358518 1.3:-45520217 MS.gene035022:CDS
TTTGTGGACACTTACAAAAC+TGG 0.364762 1.3:-45520027 MS.gene035022:CDS
CTCATAGTCCACCTTTGCCT+TGG 0.368509 1.3:+45520806 None:intergenic
GGAGTGGTGCTTACATTGTT+AGG 0.368655 1.3:-45520143 MS.gene035022:CDS
GTGACACTTGCAGGAGTATT+GGG 0.373907 1.3:-45520773 MS.gene035022:CDS
GTTGAATACAAGAATGATAA+TGG 0.375620 1.3:-45520462 MS.gene035022:CDS
ATCAATAATGATCTTCCTTC+CGG 0.379520 1.3:+45520233 None:intergenic
CATGTGCTTGCCACTAAAAT+TGG 0.379944 1.3:-45520558 MS.gene035022:CDS
CGTGACACTTGCAGGAGTAT+TGG 0.381615 1.3:-45520774 MS.gene035022:CDS
CATGGTGGAGGTGCCTTCTC+AGG 0.384431 1.3:-45520189 MS.gene035022:CDS
TTACAGATTCTGAGGTGAAT+AGG 0.390519 1.3:+45520970 None:intergenic
TGCTCGTCGTTGTATTGTGC+AGG 0.400285 1.3:-45520088 MS.gene035022:CDS
ACCTGGTCACAGATCTTGTC+AGG 0.402637 1.3:+45520936 None:intergenic
GTGCAGGTTTCTTATGCAAT+TGG 0.405905 1.3:-45520072 MS.gene035022:CDS
ACCAGAAGACTGCTGCTTAC+GGG 0.407668 1.3:-45519897 MS.gene035022:CDS
TCTTGTATTCAACGGTAACT+TGG 0.410805 1.3:+45520472 None:intergenic
AGAGCCACTTTCTGTGTTTG+TGG 0.412076 1.3:-45520043 MS.gene035022:CDS
TCAGGTAGGTTTGTGATTGG+TGG 0.413640 1.3:-45520279 MS.gene035022:CDS
GGTTGGGGTGCTCATGGTGG+AGG 0.416114 1.3:-45520201 MS.gene035022:CDS
GGATTGTGTGCACGCGAATA+GGG 0.416704 1.3:+45520430 None:intergenic
TTGTCGTCAAGGTATTTAGC+TGG 0.418579 1.3:+45520324 None:intergenic
CCTTCAGGTAGGTTTGTGAT+TGG 0.422961 1.3:-45520282 MS.gene035022:CDS
ATAAGACATGCCCATGGGTT+AGG 0.433400 1.3:-45520509 MS.gene035022:CDS
GGTGATCAAGGTCACATGTT+TGG 0.433876 1.3:-45520621 MS.gene035022:CDS
ACCATAACTCCCTCAAGCCT+TGG 0.434421 1.3:+45519819 None:intergenic
TATGGTGGTTGGGGTGCTCA+TGG 0.437298 1.3:-45520207 MS.gene035022:CDS
CTTTCTAACTTCAGTGAGCT+TGG 0.438150 1.3:+45520533 None:intergenic
GGTTGGATCCTTTCCTGAGA+AGG 0.441186 1.3:+45520176 None:intergenic
TGCTCAAGCCCAAGGCTTGA+GGG 0.445480 1.3:-45519828 None:intergenic
ATCTTCCATCTCAACCCTTC+AGG 0.447635 1.3:-45520297 MS.gene035022:CDS
ACAGTCTCCCATGTAAAATC+AGG 0.452392 1.3:+45519856 None:intergenic
CGTCGTGTTGAGTTGAAATG+AGG 0.453932 1.3:+45520409 None:intergenic
TCACCAGCTCCAATTTCCTC+AGG 0.463353 1.3:+45520639 None:intergenic
TGTGTGAGTGGCATTAGTTC+AGG 0.465165 1.3:+45520579 None:intergenic
CCACTCCTGTCCACCTTGGT+TGG 0.465451 1.3:+45520159 None:intergenic
TACCAGAAGACTGCTGCTTA+CGG 0.467085 1.3:-45519898 MS.gene035022:CDS
CATGGAGATGCGGGGCTCAC+CGG 0.468853 1.3:-45520252 MS.gene035022:CDS
CCATAACTCCCTCAAGCCTT+GGG 0.475898 1.3:+45519820 None:intergenic
GGTGGACCTCATGGAGATGC+GGG 0.478920 1.3:-45520261 MS.gene035022:CDS
GAAATTGGAGCTGGTGATCA+AGG 0.483468 1.3:-45520633 MS.gene035022:CDS
TGCCACCTCTTGTGAGGTCA+AGG 0.490427 1.3:+45519929 None:intergenic
AGGATCCAACCAAGGTGGAC+AGG 0.494928 1.3:-45520164 MS.gene035022:CDS
AGGTTGATAGCAATCATTCC+AGG 0.495973 1.3:+45519949 None:intergenic
TGCCATCCTTGATGCATGCT+TGG 0.499134 1.3:-45520907 MS.gene035022:CDS
AGGTATTTAGCTGGGACGAC+AGG 0.499951 1.3:+45520333 None:intergenic
AACTGGAAAGATTCCAGATA+AGG 0.500009 1.3:-45520010 MS.gene035022:CDS
GGGTTTGTGTCTGCTGATGT+TGG 0.504850 1.3:-45520753 MS.gene035022:CDS
TGCCCATGGGTTAGGCCTGA+TGG 0.510007 1.3:-45520501 MS.gene035022:CDS
CAAGGATGGCATCTGAAACC+TGG 0.510900 1.3:+45520919 None:intergenic
TTATTGATACCTATGGTGGT+TGG 0.512065 1.3:-45520218 MS.gene035022:CDS
AAGATCATTATTGATACCTA+TGG 0.514480 1.3:-45520225 MS.gene035022:CDS
CCCTGACAAGATCTGTGACC+AGG 0.522104 1.3:-45520937 MS.gene035022:CDS
GTGTCCACAAACACAGAAAG+TGG 0.522772 1.3:+45520039 None:intergenic
CTTGTATTCAACGGTAACTT+GGG 0.524167 1.3:+45520473 None:intergenic
GTAACTTGGGTCTTACCATC+AGG 0.526446 1.3:+45520486 None:intergenic
ATTGATACCTATGGTGGTTG+GGG 0.527612 1.3:-45520216 MS.gene035022:CDS
ATGCTCAAGCCCAAGGCTTG+AGG 0.527948 1.3:-45519829 None:intergenic
ATTATCATTCTTGTATTCAA+CGG 0.531052 1.3:+45520464 None:intergenic
GATATTGCTCAAGGAGTTCA+CGG 0.536055 1.3:-45520678 MS.gene035022:CDS
TAGTTCAGGTGTTTCGTCAG+TGG 0.542460 1.3:+45520593 None:intergenic
TTTGTGATTGGTGGACCTCA+TGG 0.544583 1.3:-45520270 MS.gene035022:CDS
TACCATCAGGCCTAACCCAT+GGG 0.547526 1.3:+45520499 None:intergenic
TGTCGTCAAGGTATTTAGCT+GGG 0.547725 1.3:+45520325 None:intergenic
ACCTACCTGAAGGGTTGAGA+TGG 0.549506 1.3:+45520292 None:intergenic
TGGTGGACCTCATGGAGATG+CGG 0.558868 1.3:-45520262 MS.gene035022:CDS
ACAAACACAGAAAGTGGCTC+TGG 0.562649 1.3:+45520045 None:intergenic
GGTGGTTGGGGTGCTCATGG+TGG 0.563645 1.3:-45520204 MS.gene035022:CDS
TGAAGGGTTGAGATGGAAGA+TGG 0.563688 1.3:+45520299 None:intergenic
AAGCCTGAGGAAATTGGAGC+TGG 0.569064 1.3:-45520642 MS.gene035022:CDS
AGCACCACTCCTGTCCACCT+TGG 0.571010 1.3:+45520155 None:intergenic
CTCCAAGCATGCATCAAGGA+TGG 0.575330 1.3:+45520905 None:intergenic
ATCATTATTGATACCTATGG+TGG 0.575435 1.3:-45520222 MS.gene035022:CDS
ATGAGCACCCCAACCACCAT+AGG 0.575628 1.3:+45520209 None:intergenic
CAAGAATGATAATGGAGCCA+TGG 0.577331 1.3:-45520454 MS.gene035022:CDS
GGAAGATGGTCTTGTCGTCA+AGG 0.577435 1.3:+45520313 None:intergenic
TTACCATCAGGCCTAACCCA+TGG 0.578836 1.3:+45520498 None:intergenic
TGTGAAGATGCTCAAGCCCA+AGG 0.581845 1.3:-45519836 MS.gene035022:CDS
GAGATGCGGGGCTCACCGGA+AGG 0.589190 1.3:-45520248 MS.gene035022:CDS
ACCTTCGTTTACAGATTCTG+AGG 0.589403 1.3:+45520962 None:intergenic
TGAAATCACAACCAAGGCAA+AGG 0.590046 1.3:-45520817 MS.gene035022:CDS
CCAATCACAAACCTACCTGA+AGG 0.592190 1.3:+45520282 None:intergenic
AACCTTGACCTCACAAGAGG+TGG 0.593643 1.3:-45519931 MS.gene035022:CDS
GGAGCAAGATAAAGAGAGCA+AGG 0.594380 1.3:-45520886 MS.gene035022:CDS
AATCACAACCAAGGCAAAGG+TGG 0.598400 1.3:-45520814 MS.gene035022:CDS
TGAATTTGCCACCTCTTGTG+AGG 0.598537 1.3:+45519923 None:intergenic
CAGAGCCCTGATATTGCTCA+AGG 0.602611 1.3:-45520687 MS.gene035022:CDS
CTTGCTCCAAGCATGCATCA+AGG 0.602878 1.3:+45520901 None:intergenic
GAACTCCTTGAGCAATATCA+GGG 0.613163 1.3:+45520682 None:intergenic
GAAAGAATAAGACATGCCCA+TGG 0.616428 1.3:-45520515 MS.gene035022:CDS
TGGAGGTGCCTTCTCAGGAA+AGG 0.621106 1.3:-45520184 MS.gene035022:CDS
CAATCACAAACCTACCTGAA+GGG 0.630070 1.3:+45520283 None:intergenic
ATCAACCTTGACCTCACAAG+AGG 0.632107 1.3:-45519934 MS.gene035022:CDS
AGATTCTGAGGTGAATAGGA+AGG 0.635764 1.3:+45520974 None:intergenic
TCCATCTCAACCCTTCAGGT+AGG 0.636963 1.3:-45520293 MS.gene035022:CDS
AAAGAATAAGACATGCCCAT+GGG 0.640830 1.3:-45520514 MS.gene035022:CDS
CCAACCAAGGTGGACAGGAG+TGG 0.650759 1.3:-45520159 MS.gene035022:CDS
ACCTCAGAATCTGTAAACGA+AGG 0.651225 1.3:-45520963 MS.gene035022:CDS
GTGGACCTCATGGAGATGCG+GGG 0.667986 1.3:-45520260 MS.gene035022:CDS
GTGGCAAGCACATGTGTGAG+TGG 0.669118 1.3:+45520567 None:intergenic
CTCAGGAAAGGATCCAACCA+AGG 0.675495 1.3:-45520172 MS.gene035022:CDS
CTTTGGTGAAATCACAACCA+AGG 0.678587 1.3:-45520823 MS.gene035022:CDS
TCACTTGAGCAAGAAGCCTG+AGG 0.682617 1.3:-45520655 MS.gene035022:CDS
GTGCACGCGAATAGGGACCA+TGG 0.688328 1.3:+45520437 None:intergenic
CCTGGTCACAGATCTTGTCA+GGG 0.689007 1.3:+45520937 None:intergenic
GTGAGCCCCGCATCTCCATG+AGG 0.689929 1.3:+45520255 None:intergenic
AGGAAAGGATCCAACCAAGG+TGG 0.705983 1.3:-45520169 MS.gene035022:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATCATTCTTGTATTCAA+CGG + chr1.3:45520351-45520370 None:intergenic 20.0%
! AAGATCATTATTGATACCTA+TGG - chr1.3:45520587-45520606 MS.gene035022:CDS 25.0%
! GTTGAATACAAGAATGATAA+TGG - chr1.3:45520350-45520369 MS.gene035022:CDS 25.0%
! TACAAAAACTAACATGGTTA+TGG - chr1.3:45519965-45519984 MS.gene035022:CDS 25.0%
AGAACCAAAATATCCTTATC+TGG + chr1.3:45520818-45520837 None:intergenic 30.0%
ATCAATAATGATCTTCCTTC+CGG + chr1.3:45520582-45520601 None:intergenic 30.0%
ATCATTATTGATACCTATGG+TGG - chr1.3:45520590-45520609 MS.gene035022:CDS 30.0%
GACATGTACAAAAACTAACA+TGG - chr1.3:45519959-45519978 MS.gene035022:CDS 30.0%
! GATTCCAGATAAGGATATTT+TGG - chr1.3:45520811-45520830 MS.gene035022:CDS 30.0%
!!! GGATATTTTGGTTCTGATTA+AGG - chr1.3:45520823-45520842 MS.gene035022:CDS 30.0%
!!! TTAAGGAGAATTTTGACTTC+AGG - chr1.3:45520840-45520859 MS.gene035022:CDS 30.0%
AAAGAATAAGACATGCCCAT+GGG - chr1.3:45520298-45520317 MS.gene035022:CDS 35.0%
AACTGGAAAGATTCCAGATA+AGG - chr1.3:45520802-45520821 MS.gene035022:CDS 35.0%
ACTAACATGGTTATGGTCTT+TGG - chr1.3:45519972-45519991 MS.gene035022:CDS 35.0%
CTTGTATTCAACGGTAACTT+GGG + chr1.3:45520342-45520361 None:intergenic 35.0%
TATTGATACCTATGGTGGTT+GGG - chr1.3:45520595-45520614 MS.gene035022:CDS 35.0%
TCTTGTATTCAACGGTAACT+TGG + chr1.3:45520343-45520362 None:intergenic 35.0%
TTATTGATACCTATGGTGGT+TGG - chr1.3:45520594-45520613 MS.gene035022:CDS 35.0%
TTTGTGGACACTTACAAAAC+TGG - chr1.3:45520785-45520804 MS.gene035022:CDS 35.0%
! TGACGATCCTGATTTTACAT+GGG - chr1.3:45520949-45520968 MS.gene035022:CDS 35.0%
!! TTACAGATTCTGAGGTGAAT+AGG + chr1.3:45519845-45519864 None:intergenic 35.0%
AAATAGTTCGTGACACTTGC+AGG - chr1.3:45520030-45520049 MS.gene035022:CDS 40.0%
ACAGTCTCCCATGTAAAATC+AGG + chr1.3:45520959-45520978 None:intergenic 40.0%
ACCTCAGAATCTGTAAACGA+AGG - chr1.3:45519849-45519868 MS.gene035022:CDS 40.0%
ACCTTCGTTTACAGATTCTG+AGG + chr1.3:45519853-45519872 None:intergenic 40.0%
ATTGATACCTATGGTGGTTG+GGG - chr1.3:45520596-45520615 MS.gene035022:CDS 40.0%
CAATCACAAACCTACCTGAA+GGG + chr1.3:45520532-45520551 None:intergenic 40.0%
CATGTGCTTGCCACTAAAAT+TGG - chr1.3:45520254-45520273 MS.gene035022:CDS 40.0%
CTTTCTAACTTCAGTGAGCT+TGG + chr1.3:45520282-45520301 None:intergenic 40.0%
CTTTGGTGAAATCACAACCA+AGG - chr1.3:45519989-45520008 MS.gene035022:CDS 40.0%
GAAAGAATAAGACATGCCCA+TGG - chr1.3:45520297-45520316 MS.gene035022:CDS 40.0%
GAACTCCTTGAGCAATATCA+GGG + chr1.3:45520133-45520152 None:intergenic 40.0%
GATATTGCTCAAGGAGTTCA+CGG - chr1.3:45520134-45520153 MS.gene035022:CDS 40.0%
GTGCAGGTTTCTTATGCAAT+TGG - chr1.3:45520740-45520759 MS.gene035022:CDS 40.0%
TGAAATCACAACCAAGGCAA+AGG - chr1.3:45519995-45520014 MS.gene035022:CDS 40.0%
TGAACTCCTTGAGCAATATC+AGG + chr1.3:45520134-45520153 None:intergenic 40.0%
! AGTTTTTGTACATGTCTCGC+AGG + chr1.3:45519955-45519974 None:intergenic 40.0%
! GTGACGATCCTGATTTTACA+TGG - chr1.3:45520948-45520967 MS.gene035022:CDS 40.0%
! TGTCGTCAAGGTATTTAGCT+GGG + chr1.3:45520490-45520509 None:intergenic 40.0%
! TTGTCGTCAAGGTATTTAGC+TGG + chr1.3:45520491-45520510 None:intergenic 40.0%
!! AGATTCTGAGGTGAATAGGA+AGG + chr1.3:45519841-45519860 None:intergenic 40.0%
!! AGGTTGATAGCAATCATTCC+AGG + chr1.3:45520866-45520885 None:intergenic 40.0%
!! CAAAAAGTGTTGTCGCTTCA+GGG - chr1.3:45520699-45520718 MS.gene035022:CDS 40.0%
!! CAAGAATGATAATGGAGCCA+TGG - chr1.3:45520358-45520377 MS.gene035022:CDS 40.0%
AATCACAACCAAGGCAAAGG+TGG - chr1.3:45519998-45520017 MS.gene035022:CDS 45.0%
ACAAACACAGAAAGTGGCTC+TGG + chr1.3:45520770-45520789 None:intergenic 45.0%
AGCAAGAAGCCTGAGGAAAT+TGG - chr1.3:45520164-45520183 MS.gene035022:CDS 45.0%
ATAAGACATGCCCATGGGTT+AGG - chr1.3:45520303-45520322 MS.gene035022:CDS 45.0%
ATCAACCTTGACCTCACAAG+AGG - chr1.3:45520878-45520897 MS.gene035022:CDS 45.0%
ATCTTCCATCTCAACCCTTC+AGG - chr1.3:45520515-45520534 MS.gene035022:CDS 45.0%
CCAATCACAAACCTACCTGA+AGG + chr1.3:45520533-45520552 None:intergenic 45.0%
CCTTCAGGTAGGTTTGTGAT+TGG - chr1.3:45520530-45520549 MS.gene035022:CDS 45.0%
GAAATTGGAGCTGGTGATCA+AGG - chr1.3:45520179-45520198 MS.gene035022:CDS 45.0%
GGAGCAAGATAAAGAGAGCA+AGG - chr1.3:45519926-45519945 MS.gene035022:CDS 45.0%
GGAGTGGTGCTTACATTGTT+AGG - chr1.3:45520669-45520688 MS.gene035022:CDS 45.0%
GGTGATCAAGGTCACATGTT+TGG - chr1.3:45520191-45520210 MS.gene035022:CDS 45.0%
GTAACTTGGGTCTTACCATC+AGG + chr1.3:45520329-45520348 None:intergenic 45.0%
GTGTCCACAAACACAGAAAG+TGG + chr1.3:45520776-45520795 None:intergenic 45.0%
TAGTTCAGGTGTTTCGTCAG+TGG + chr1.3:45520222-45520241 None:intergenic 45.0%
TCACAAGAGGTGGCAAATTC+AGG - chr1.3:45520891-45520910 MS.gene035022:CDS 45.0%
TCAGGTAGGTTTGTGATTGG+TGG - chr1.3:45520533-45520552 MS.gene035022:CDS 45.0%
TGAAGGGTTGAGATGGAAGA+TGG + chr1.3:45520516-45520535 None:intergenic 45.0%
TGAATTTGCCACCTCTTGTG+AGG + chr1.3:45520892-45520911 None:intergenic 45.0%
TGTGTGAGTGGCATTAGTTC+AGG + chr1.3:45520236-45520255 None:intergenic 45.0%
! AGAGCCACTTTCTGTGTTTG+TGG - chr1.3:45520769-45520788 MS.gene035022:CDS 45.0%
! GAGCTTGGCACCAATTTTAG+TGG + chr1.3:45520267-45520286 None:intergenic 45.0%
! TACCAGAAGACTGCTGCTTA+CGG - chr1.3:45520914-45520933 MS.gene035022:CDS 45.0%
! TTTGTGATTGGTGGACCTCA+TGG - chr1.3:45520542-45520561 MS.gene035022:CDS 45.0%
!! CGTCGTGTTGAGTTGAAATG+AGG + chr1.3:45520406-45520425 None:intergenic 45.0%
!! GCAAAAAGTGTTGTCGCTTC+AGG - chr1.3:45520698-45520717 MS.gene035022:CDS 45.0%
!! GTGACACTTGCAGGAGTATT+GGG - chr1.3:45520039-45520058 MS.gene035022:CDS 45.0%
!!! GAGAATTTTGACTTCAGGCC+TGG - chr1.3:45520845-45520864 MS.gene035022:CDS 45.0%
AACCTTGACCTCACAAGAGG+TGG - chr1.3:45520881-45520900 MS.gene035022:CDS 50.0%
AAGCCTGAGGAAATTGGAGC+TGG - chr1.3:45520170-45520189 MS.gene035022:CDS 50.0%
ACCTACCTGAAGGGTTGAGA+TGG + chr1.3:45520523-45520542 None:intergenic 50.0%
ACCTGGTCACAGATCTTGTC+AGG + chr1.3:45519879-45519898 None:intergenic 50.0%
AGGAAAGGATCCAACCAAGG+TGG - chr1.3:45520643-45520662 MS.gene035022:CDS 50.0%
AGGATTGTGTGCACGCGAAT+AGG + chr1.3:45520386-45520405 None:intergenic 50.0%
AGGTATTTAGCTGGGACGAC+AGG + chr1.3:45520482-45520501 None:intergenic 50.0%
CAGAGCCCTGATATTGCTCA+AGG - chr1.3:45520125-45520144 MS.gene035022:CDS 50.0%
CCTGGTCACAGATCTTGTCA+GGG + chr1.3:45519878-45519897 None:intergenic 50.0%
CTCAGGAAAGGATCCAACCA+AGG - chr1.3:45520640-45520659 MS.gene035022:CDS 50.0%
CTCATAGTCCACCTTTGCCT+TGG + chr1.3:45520009-45520028 None:intergenic 50.0%
CTCCAAGCATGCATCAAGGA+TGG + chr1.3:45519910-45519929 None:intergenic 50.0%
CTTGCTCCAAGCATGCATCA+AGG + chr1.3:45519914-45519933 None:intergenic 50.0%
GGATTGTGTGCACGCGAATA+GGG + chr1.3:45520385-45520404 None:intergenic 50.0%
GGGTTTGTGTCTGCTGATGT+TGG - chr1.3:45520059-45520078 MS.gene035022:CDS 50.0%
GGTTGGATCCTTTCCTGAGA+AGG + chr1.3:45520639-45520658 None:intergenic 50.0%
TACCATCAGGCCTAACCCAT+GGG + chr1.3:45520316-45520335 None:intergenic 50.0%
TCACCAGCTCCAATTTCCTC+AGG + chr1.3:45520176-45520195 None:intergenic 50.0%
TCACTTGAGCAAGAAGCCTG+AGG - chr1.3:45520157-45520176 MS.gene035022:CDS 50.0%
TCCATCTCAACCCTTCAGGT+AGG - chr1.3:45520519-45520538 MS.gene035022:CDS 50.0%
TGCCATCCTTGATGCATGCT+TGG - chr1.3:45519905-45519924 MS.gene035022:CDS 50.0%
TGCTCGTCGTTGTATTGTGC+AGG - chr1.3:45520724-45520743 MS.gene035022:CDS 50.0%
TGTGAAGATGCTCAAGCCCA+AGG - chr1.3:45520976-45520995 MS.gene035022:CDS 50.0%
TTACCATCAGGCCTAACCCA+TGG + chr1.3:45520317-45520336 None:intergenic 50.0%
! ACCAGAAGACTGCTGCTTAC+GGG - chr1.3:45520915-45520934 MS.gene035022:CDS 50.0%
! CAAGGATGGCATCTGAAACC+TGG + chr1.3:45519896-45519915 None:intergenic 50.0%
! CGTGACACTTGCAGGAGTAT+TGG - chr1.3:45520038-45520057 MS.gene035022:CDS 50.0%
!! ACTGCTGCTTACGGGCATTT+TGG - chr1.3:45520923-45520942 MS.gene035022:CDS 50.0%
!! GGAAGATGGTCTTGTCGTCA+AGG + chr1.3:45520502-45520521 None:intergenic 50.0%
AGGATCCAACCAAGGTGGAC+AGG - chr1.3:45520648-45520667 MS.gene035022:CDS 55.0%
ATGAGCACCCCAACCACCAT+AGG + chr1.3:45520606-45520625 None:intergenic 55.0%
CCCTGACAAGATCTGTGACC+AGG - chr1.3:45519875-45519894 MS.gene035022:CDS 55.0%
GTGGCAAGCACATGTGTGAG+TGG + chr1.3:45520248-45520267 None:intergenic 55.0%
TGCCACCTCTTGTGAGGTCA+AGG + chr1.3:45520886-45520905 None:intergenic 55.0%
! TATGGTGGTTGGGGTGCTCA+TGG - chr1.3:45520605-45520624 MS.gene035022:CDS 55.0%
! TGGTGGACCTCATGGAGATG+CGG - chr1.3:45520550-45520569 MS.gene035022:CDS 55.0%
!! TGGAGGTGCCTTCTCAGGAA+AGG - chr1.3:45520628-45520647 MS.gene035022:CDS 55.0%
AGCACCACTCCTGTCCACCT+TGG + chr1.3:45520660-45520679 None:intergenic 60.0%
CCAACCAAGGTGGACAGGAG+TGG - chr1.3:45520653-45520672 MS.gene035022:CDS 60.0%
CCACTCCTGTCCACCTTGGT+TGG + chr1.3:45520656-45520675 None:intergenic 60.0%
GCCCGTAAGCAGCAGTCTTC+TGG + chr1.3:45520919-45520938 None:intergenic 60.0%
GGTGGACCTCATGGAGATGC+GGG - chr1.3:45520551-45520570 MS.gene035022:CDS 60.0%
GTGCACGCGAATAGGGACCA+TGG + chr1.3:45520378-45520397 None:intergenic 60.0%
GTGGACCTCATGGAGATGCG+GGG - chr1.3:45520552-45520571 MS.gene035022:CDS 60.0%
!! CATGGTGGAGGTGCCTTCTC+AGG - chr1.3:45520623-45520642 MS.gene035022:CDS 60.0%
!! TGCCCATGGGTTAGGCCTGA+TGG - chr1.3:45520311-45520330 MS.gene035022:CDS 60.0%
CATGGAGATGCGGGGCTCAC+CGG - chr1.3:45520560-45520579 MS.gene035022:CDS 65.0%
GTGAGCCCCGCATCTCCATG+AGG + chr1.3:45520560-45520579 None:intergenic 65.0%
! GGTGGTTGGGGTGCTCATGG+TGG - chr1.3:45520608-45520627 MS.gene035022:CDS 65.0%
! GGTTGGGGTGCTCATGGTGG+AGG - chr1.3:45520611-45520630 MS.gene035022:CDS 65.0%
GAGATGCGGGGCTCACCGGA+AGG - chr1.3:45520564-45520583 MS.gene035022:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 45519831 45521003 45519831 ID=MS.gene035022
chr1.3 mRNA 45519831 45521003 45519831 ID=MS.gene035022.t1;Parent=MS.gene035022
chr1.3 exon 45519831 45521003 45519831 ID=MS.gene035022.t1.exon1;Parent=MS.gene035022.t1
chr1.3 CDS 45519831 45521003 45519831 ID=cds.MS.gene035022.t1;Parent=MS.gene035022.t1
Gene Sequence

>MS.gene035022

ATGGATACCTTCCTATTCACCTCAGAATCTGTAAACGAAGGTCACCCTGACAAGATCTGTGACCAGGTTTCAGATGCCATCCTTGATGCATGCTTGGAGCAAGATAAAGAGAGCAAGGTTGCCTGCGAGACATGTACAAAAACTAACATGGTTATGGTCTTTGGTGAAATCACAACCAAGGCAAAGGTGGACTATGAGAAAATAGTTCGTGACACTTGCAGGAGTATTGGGTTTGTGTCTGCTGATGTTGGTCTTGATGCTGACAAATGCAATGTTCTCGTCAACATTGAGCAACAGAGCCCTGATATTGCTCAAGGAGTTCACGGTCACTTGAGCAAGAAGCCTGAGGAAATTGGAGCTGGTGATCAAGGTCACATGTTTGGCTATGCCACTGACGAAACACCTGAACTAATGCCACTCACACATGTGCTTGCCACTAAAATTGGTGCCAAGCTCACTGAAGTTAGAAAGAATAAGACATGCCCATGGGTTAGGCCTGATGGTAAGACCCAAGTTACCGTTGAATACAAGAATGATAATGGAGCCATGGTCCCTATTCGCGTGCACACAATCCTCATTTCAACTCAACACGACGAAACTGTCACAAATGAAAAGATTGCTCAAGATTTGAAAGAGCATGTTATTAAACCTGTCGTCCCAGCTAAATACCTTGACGACAAGACCATCTTCCATCTCAACCCTTCAGGTAGGTTTGTGATTGGTGGACCTCATGGAGATGCGGGGCTCACCGGAAGGAAGATCATTATTGATACCTATGGTGGTTGGGGTGCTCATGGTGGAGGTGCCTTCTCAGGAAAGGATCCAACCAAGGTGGACAGGAGTGGTGCTTACATTGTTAGGCAAGCTGCAAAAAGTGTTGTCGCTTCAGGGCTTGCTCGTCGTTGTATTGTGCAGGTTTCTTATGCAATTGGAGTGCCAGAGCCACTTTCTGTGTTTGTGGACACTTACAAAACTGGAAAGATTCCAGATAAGGATATTTTGGTTCTGATTAAGGAGAATTTTGACTTCAGGCCTGGAATGATTGCTATCAACCTTGACCTCACAAGAGGTGGCAAATTCAGGTACCAGAAGACTGCTGCTTACGGGCATTTTGGACGTGACGATCCTGATTTTACATGGGAGACTGTGAAGATGCTCAAGCCCAAGGCTTGA
Protein sequence

>MS.gene035022.t1

MDTFLFTSESVNEGHPDKICDQVSDAILDACLEQDKESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSADVGLDADKCNVLVNIEQQSPDIAQGVHGHLSKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKIGAKLTEVRKNKTCPWVRPDGKTQVTVEYKNDNGAMVPIRVHTILISTQHDETVTNEKIAQDLKEHVIKPVVPAKYLDDKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSVVASGLARRCIVQVSYAIGVPEPLSVFVDTYKTGKIPDKDILVLIKENFDFRPGMIAINLDLTRGGKFRYQKTAAYGHFGRDDPDFTWETVKMLKPKA