AlfalfaGEDB | MS.gene035276

M. sativa cultivar XinJiangDaYe / MS.gene035276

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF01535.23 PF13041.9 PF20431.1

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035276.t1	PNX99292.1	87.5	584	72	1	1	584	42	624	1.30E-274	955.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035276.t1	Q9SJK9	52.4	580	270	4	1	575	41	619	4.8e-148	526.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035276.t1	A0A2K3N8D1	87.5	584	72	1	1	584	42	624	9.5e-275	955.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI:

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Gene1	Gene2	Type
MS.gene002077	MS.gene035276	PPI
MS.gene003811	MS.gene035276	PPI
MS.gene017626	MS.gene035276	PPI
MS.gene035276	MS.gene005172	PPI
MS.gene035276	MS.gene21042	PPI
MS.gene035276	MS.gene30781	PPI
MS.gene035276	MS.gene033840	PPI
MS.gene035276	MS.gene02626	PPI
MS.gene035276	MS.gene069541	PPI
MS.gene035276	MS.gene99250	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035276.t1	MTR_4g118700	96.233	584	22	0	1	584	42	625	0.0	1174
MS.gene035276.t1	MTR_4g118700	27.168	346	220	2	102	415	10	355	3.14e-39	152
MS.gene035276.t1	MTR_3g096420	35.121	578	330	6	11	576	126	670	2.46e-109	343
MS.gene035276.t1	MTR_3g096420	25.714	420	245	4	30	415	18	404	7.30e-45	169
MS.gene035276.t1	MTR_3g096420	30.588	255	177	0	162	416	18	272	8.02e-37	146
MS.gene035276.t1	MTR_1g073300	32.660	594	377	8	1	574	110	700	1.89e-100	322
MS.gene035276.t1	MTR_1g073300	26.316	456	280	10	53	454	27	480	2.76e-35	142
MS.gene035276.t1	MTR_3g084440	34.019	535	317	9	43	574	20	521	1.64e-99	315
MS.gene035276.t1	MTR_3g084440	25.905	359	230	5	28	381	106	433	3.64e-32	131
MS.gene035276.t1	MTR_3g084440	27.835	194	103	2	17	173	230	423	1.11e-12	71.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035276.t1	AT2G36980	51.724	580	274	4	1	575	41	619	0.0	629
MS.gene035276.t1	AT2G36980	27.841	352	220	3	98	415	5	356	2.35e-39	153
MS.gene035276.t1	AT2G36980	51.724	580	274	4	1	575	32	610	0.0	628
MS.gene035276.t1	AT2G36980	28.107	338	209	3	112	415	10	347	8.41e-39	151
MS.gene035276.t1	AT2G36980	29.293	198	107	3	243	407	9	206	1.81e-17	86.7
MS.gene035276.t1	AT2G36980	51.502	466	223	2	113	575	2	467	3.21e-176	507
MS.gene035276.t1	AT2G36980	24.719	356	226	5	1	350	23	342	2.83e-30	124
MS.gene035276.t1	AT2G13600	35.326	552	317	4	30	574	151	669	4.36e-108	340
MS.gene035276.t1	AT2G13600	25.778	450	257	6	12	418	4	419	5.88e-41	158
MS.gene035276.t1	AT2G13600	29.110	292	206	1	162	452	18	309	1.18e-36	145

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Find 127 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGAAGATCAGATGCAC+CGG	0.614078	8.1:-9165778	None:intergenic
AAACACTTGTAGGCTTGATA+TGG	0.348400	8.1:+9165977	MS.gene035276:CDS
AAAGGAAATGATGGACCAAG+GGG	0.697644	8.1:+9167222	MS.gene035276:CDS
AAATAATTTATATCCTTGCA+TGG	0.555466	8.1:+9167309	MS.gene035276:CDS
AACGCGCTGATTGATATGTA+TGG	0.493281	8.1:+9165861	MS.gene035276:CDS
AACTCATGTGAGGTTCTACT+TGG	0.511570	8.1:+9167052	MS.gene035276:CDS
AAGTAGAACCTCACATGAGT+TGG	0.619948	8.1:-9167050	None:intergenic
AAGTTTGTTTGTAAAGTCCA+AGG	0.551639	8.1:-9165674	None:intergenic
AATGGATCATGTGGCATGCA+TGG	0.606188	8.1:+9166946	MS.gene035276:CDS
AATTAGAAATGAGACACACA+CGG	0.710038	8.1:+9167362	MS.gene035276:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTAGAAATGAGACACACA+CGG	+	chr8.1:9167362-9167381	MS.gene035276:CDS	30.0%
CAAATATGTATTGTGCAAGT+GGG	+	chr8.1:9167173-9167192	MS.gene035276:CDS	30.0%
CGGAGAAAAATATTGTATCT+TGG	+	chr8.1:9166420-9166439	MS.gene035276:CDS	30.0%
GAGTCGATGGAATTATTATA+CGG	+	chr8.1:9166176-9166195	MS.gene035276:CDS	30.0%
GATAGTAAGAAAGGAAATGA+TGG	+	chr8.1:9167213-9167232	MS.gene035276:CDS	30.0%
TATATGCATGTTCAGAGAAA+TGG	+	chr8.1:9166793-9166812	MS.gene035276:CDS	30.0%
TCAAATATGTATTGTGCAAG+TGG	+	chr8.1:9167172-9167191	MS.gene035276:CDS	30.0%
TGCAGAGTATGAGAAATTAT+CGG	-	chr8.1:9165742-9165761	None:intergenic	30.0%
TGCTTGGAATATAGTTATTG+CGG	+	chr8.1:9166040-9166059	MS.gene035276:CDS	30.0%
TGGAACAAAAGTTCATTCTT+TGG	+	chr8.1:9165800-9165819	MS.gene035276:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	9165654	9167408	9165654	ID=MS.gene035276
chr8.1	mRNA	9165654	9167408	9165654	ID=MS.gene035276.t1;Parent=MS.gene035276
chr8.1	exon	9165654	9167408	9165654	ID=MS.gene035276.t1.exon1;Parent=MS.gene035276.t1
chr8.1	CDS	9165654	9167408	9165654	ID=cds.MS.gene035276.t1;Parent=MS.gene035276.t1

Gene Sequence

>MS.gene035276

ATGTTAACTGCCTATTCCCGCCTTGGACTTTACAAACAAACTTTTGATCTCTTCGATAGCATGAGGAGAATCTCCGATTCCAAACCCGATAATTTCTCATACTCTGCAGCCATAAATTCATGTGCCGGTGCATCTGATCTTCGTTTTGGAACAAAAGTTCATTCTTTGGTTGTTGTTTCAGGGTATCCGTCTTCTCTTCCTGTCGCTAACGCGCTGATTGATATGTATGGAAAGTGTTTCAATCCGAATGATGCAAGAAAAGTGTTTGATGAGATGAATTATAGTAATGAAGTGACATGGTGTTCACTTCTGTTTGCTTATGCAAACACTTGTAGGCTTGATATGGCTTTTGAAATTTTTCGTAGCATGCCTGAAAAAGTTGAAGTTGCTTGGAATATAGTTATTGCGGTTCATGCTCGTTGTGGTGAAGTTGAGGCATGCTTGCATTTGTTTAAAGAGATGTGTGAGGGTTTGTATCAGCCAGATCAATGGACTTTCAGTGCTCTTATGAGTGCTTGTGCTGAGTCGATGGAATTATTATACGGATGTATGATGCATTGTTTTGTGATTAAATCTGGTTGGAGTACTGCTATGGAGGTAAATAACTCAATTGTAAGCTTTTATGCTAAATTAGAATGTCATGGTGATGCTGTGAAGGTGTTTAACTCTGGTGGTGCTTTTAATCAAGTGTCTTGGAATGCGATCATTGATGCGCATATGAAAGTGGGGGAGACACAGAAAGCTCTTCTTGCTTTTCAGCAAGCCCCGGAGAAAAATATTGTATCTTGGACTTCTATGATTGTTGGGTATACGAGAAATGGGAATGGAGATTTAGCTTTAAGTTTGTTTTTAGACATGAAAAGGAACTCTTTCCAGCTTGATGATTTAGTAGCTGGAGCGGTCCTTCATGCTTGTGCTAGCTTGGCAATCTTAGTTCATGGAAAAATGGTCCACAGTTGCATTATTCATCTTGGTTTAGACAAGTATTTATTTGTTGGCAATAGCTTGATTAATATGTATGCTAAGTGTGGTGACATAGAAGGTTCAAAGCTCGCTTTTTGTGGCATTAATGACAAGGACCTGGTTTCCTGGAATTCAATGTTGTTTGCGTTTGGACTAAATGGGAGGGGTAATGAAGCTATATGCATGTTCAGAGAAATGGTGGCATCTGGTGTAAGTCCTGATGTAGTAACCTTCACAGGGCTGCTAATGACTTGCAGCCATTTGGGGCTTATTGATGAAGGATTTGCTTTCTTTCAATCAATGAGTTTGGAATATGGACTTGTCCAAGGAATGGATCATGTGGCATGCATGGTGGATATGCTTGGTCGAGGCGGATATGTAGCCGAAGCACAAAGTTTAGCTAAGAAGTATTCAAAGACGAGTAGAGATAAGACCAACTCATGTGAGGTTCTACTTGGTGCATGTCATGCACATAGTGATTTAGGAACAGGGAGCTCTGTGGCGGAATATGTAAAGAATTTAGAGCCTAAGAAAGAGGTTGGTTATGTATTGTTGTCAAATATGTATTGTGCAAGTGGGATGTGGAAGGAGGCAGAGATAGTAAGAAAGGAAATGATGGACCAAGGGGTGAAGAAAGTGCCCGGCTGTAGTTGGATTGAGATAAGAAATGTGGTGACTGCATTTGTATCAGGAAATAATTTATATCCTTGCATGGCTGATATATCTAAGATACTATACTTCCTTGAATTAGAAATGAGACACACACGGCCCATTAATTTTGATATTGAATGA

Protein sequence

>MS.gene035276.t1

MLTAYSRLGLYKQTFDLFDSMRRISDSKPDNFSYSAAINSCAGASDLRFGTKVHSLVVVSGYPSSLPVANALIDMYGKCFNPNDARKVFDEMNYSNEVTWCSLLFAYANTCRLDMAFEIFRSMPEKVEVAWNIVIAVHARCGEVEACLHLFKEMCEGLYQPDQWTFSALMSACAESMELLYGCMMHCFVIKSGWSTAMEVNNSIVSFYAKLECHGDAVKVFNSGGAFNQVSWNAIIDAHMKVGETQKALLAFQQAPEKNIVSWTSMIVGYTRNGNGDLALSLFLDMKRNSFQLDDLVAGAVLHACASLAILVHGKMVHSCIIHLGLDKYLFVGNSLINMYAKCGDIEGSKLAFCGINDKDLVSWNSMLFAFGLNGRGNEAICMFREMVASGVSPDVVTFTGLLMTCSHLGLIDEGFAFFQSMSLEYGLVQGMDHVACMVDMLGRGGYVAEAQSLAKKYSKTSRDKTNSCEVLLGACHAHSDLGTGSSVAEYVKNLEPKKEVGYVLLSNMYCASGMWKEAEIVRKEMMDQGVKKVPGCSWIEIRNVVTAFVSGNNLYPCMADISKILYFLELEMRHTRPINFDIE