AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene038064

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038064.t1 MTR_1g069575 94.192 396 19 1 12 403 12 407 0.0 752
MS.gene038064.t1 MTR_4g084040 41.265 332 172 5 81 395 30 355 1.20e-78 247
MS.gene038064.t1 MTR_4g084040 41.390 331 171 5 82 395 1 325 1.79e-78 245
MS.gene038064.t1 MTR_4g084040 47.788 226 114 1 170 395 38 259 7.74e-68 216
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038064.t1 AT5G44000 67.614 352 104 3 56 398 44 394 4.26e-177 499
MS.gene038064.t1 AT4G19880 39.766 342 183 5 70 394 20 355 2.90e-74 236
MS.gene038064.t1 AT5G45020 38.739 333 177 6 82 395 1 325 2.20e-71 227
MS.gene038064.t1 AT5G45020 38.739 333 177 6 82 395 1 325 2.20e-71 227
MS.gene038064.t1 AT5G44990 39.117 317 170 6 95 394 11 321 5.53e-69 222
MS.gene038064.t1 AT5G44990 39.117 317 170 6 95 394 11 321 5.53e-69 222
MS.gene038064.t1 AT4G19880 36.957 368 183 6 70 394 20 381 1.29e-68 222
MS.gene038064.t1 AT5G44990 42.795 229 127 1 170 398 18 242 1.25e-59 195
MS.gene038064.t1 AT4G19880 40.230 261 137 4 70 313 20 278 1.73e-55 186
MS.gene038064.t1 AT5G44990 36.803 269 142 7 95 346 11 268 2.70e-52 176

Find 149 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTTAATTCCGGGTTAAA+TGG 0.145587 1.2:-52647979 MS.gene038064:CDS
CCGCTTTATCATATGCTTCT+TGG 0.182145 1.2:+52647551 None:intergenic
TGAACCAGTATTTGTTAAAT+AGG 0.222094 1.2:-52647066 None:intergenic
ATAATTCAATTGTTTAATTC+CGG 0.258778 1.2:-52647988 MS.gene038064:CDS
CTCATTACATACAACATCTT+TGG 0.263092 1.2:+52648020 None:intergenic
ATTACAAGTATCTGCAACTT+TGG 0.276080 1.2:+52647226 None:intergenic
TCTTTCACCACCCGAGTTAA+AGG 0.284660 1.2:-52647932 MS.gene038064:CDS
AGACTTTGAAGGAAGTTTAC+AGG 0.293983 1.2:-52648101 MS.gene038064:CDS
TACACACCATTGTTCACATT+TGG 0.307861 1.2:+52647877 None:intergenic
TGGACCGGAGAATCGGAATT+TGG 0.312564 1.2:+52648326 None:intergenic
GTTCTGTTACAAAACTTCTC+TGG 0.315841 1.2:-52648473 MS.gene038064:CDS
ATTGCCTATTTAACAAATAC+TGG 0.329847 1.2:+52647062 None:intergenic
TAATTCAATTGTTTAATTCC+GGG 0.335485 1.2:-52647987 MS.gene038064:CDS
GAGCTGCCACGTGGAGTTCC+AGG 0.336140 1.2:+52648404 None:intergenic
TCTGAAGTTGAAGGCATAAT+TGG 0.346959 1.2:+52647140 None:intergenic
GACAAGGTGGTGTGAGGAAG+AGG 0.351548 1.2:+52648605 None:intergenic
TCGGGCAATTAGTCCGAGTT+TGG 0.357269 1.2:-52648142 MS.gene038064:CDS
TCGGAATTTGGAAGCGGGTC+TGG 0.361164 1.2:+52648338 None:intergenic
ATACTCATACAACTTCTTCT+TGG 0.361565 1.2:+52647378 None:intergenic
CGCCGGGAATGGACGGGTCG+TGG 0.375710 1.2:-52648191 MS.gene038064:CDS
AAGATCCAAGTCCGGGTTAT+GGG 0.387190 1.2:+52647951 None:intergenic
TTCAAACGAGTTGGCGATAC+GGG 0.393026 1.2:-52648165 MS.gene038064:CDS
CTGGGGTCGGTCCCTCCCAC+CGG 0.396579 1.2:-52648454 MS.gene038064:CDS
TTACAAGTATCTGCAACTTT+GGG 0.397228 1.2:+52647227 None:intergenic
TTCTGTTACAAAACTTCTCT+GGG 0.401395 1.2:-52648472 MS.gene038064:CDS
GGTGGGCCCACGGGCATGGC+AGG 0.401801 1.2:+52648274 None:intergenic
GTCTGTTTACTACTTTGATT+CGG 0.402179 1.2:-52647435 MS.gene038064:CDS
TCCCTGGGTTGAGAGGAAAA+AGG 0.407425 1.2:+52647171 None:intergenic
AAAGATCCAAGTCCGGGTTA+TGG 0.407434 1.2:+52647950 None:intergenic
GGAATATCCGCCGGTGGGGT+CGG 0.408123 1.2:+52648359 None:intergenic
TTCTTTGCATGCCTCATGGC+AGG 0.410450 1.2:-52647109 MS.gene038064:CDS
TCCCACGACCCGTCCATTCC+CGG 0.410480 1.2:+52648189 None:intergenic
CTACATGCGTGACATTTATC+AGG 0.410736 1.2:-52647341 MS.gene038064:intron
CGACAACCACTTTCCGCAAC+CGG 0.426378 1.2:-52648572 MS.gene038064:CDS
ACCGGTGTCAGTGGCATCGC+CGG 0.428200 1.2:-52648208 MS.gene038064:CDS
ATTTCATGCTCTGAAGTTGA+AGG 0.430518 1.2:+52647131 None:intergenic
AATTAGTCCGAGTTTGGATA+AGG 0.434261 1.2:-52648136 MS.gene038064:CDS
TGTTTAATTCCGGGTTAAAT+GGG 0.435774 1.2:-52647978 MS.gene038064:CDS
GTCAGTGGCATCGCCGGGAA+TGG 0.437517 1.2:-52648202 MS.gene038064:CDS
CTGGTTCAGTTGATGATGAT+AGG 0.444961 1.2:+52647081 None:intergenic
AGAACGGATGAGGGAACTTC+CGG 0.445710 1.2:+52648542 None:intergenic
TATCCGCCGGTGGGGTCGGA+GGG 0.453806 1.2:+52648363 None:intergenic
TTCCGGTGAGTCTCCGGTTG+CGG 0.460175 1.2:+52648559 None:intergenic
TCACAACCGTCCGCACCGCC+TGG 0.467210 1.2:-52648422 MS.gene038064:CDS
TGTTGTCAAGGGACATAGTC+AGG 0.469351 1.2:+52648517 None:intergenic
GTTGGCCCATAACCCGGACT+TGG 0.471548 1.2:-52647956 MS.gene038064:CDS
ATATCCGCCGGTGGGGTCGG+AGG 0.471724 1.2:+52648362 None:intergenic
ACGAGTTGGCGATACGGGTC+GGG 0.473229 1.2:-52648160 MS.gene038064:CDS
GTGGCATCGCCGGGAATGGA+CGG 0.474114 1.2:-52648198 MS.gene038064:CDS
TCGGCGGTGGACCGGAGAAT+CGG 0.477661 1.2:+52648319 None:intergenic
CTCAATCTTATCCTTTAACT+CGG 0.479397 1.2:+52647921 None:intergenic
TCAATCTTATCCTTTAACTC+GGG 0.490673 1.2:+52647922 None:intergenic
GGGAACTTCCGGTGAGTCTC+CGG 0.493773 1.2:+52648553 None:intergenic
TAATTGGTCTAATGCTCCCT+GGG 0.497106 1.2:+52647156 None:intergenic
TAATTCCGGGTTAAATGGGT+TGG 0.498858 1.2:-52647974 MS.gene038064:CDS
CTGAGAAGTGGTGGGTATGA+TGG 0.499496 1.2:-52648078 MS.gene038064:CDS
ATTCAAACGAGTTGGCGATA+CGG 0.505943 1.2:-52648166 MS.gene038064:CDS
TAGTCAGGCGAGAACGGATG+AGG 0.506765 1.2:+52648532 None:intergenic
ATGTGAACAATGGTGTGTAT+AGG 0.507417 1.2:-52647873 MS.gene038064:intron
GTACGGTGGGCCCACGGGCA+TGG 0.510535 1.2:+52648270 None:intergenic
TGGTTCAGTTGATGATGATA+GGG 0.511787 1.2:+52647082 None:intergenic
CTGATAAATGTCACGCATGT+AGG 0.512267 1.2:+52647342 None:intergenic
GAATGGTTGCAAGACTTTGA+AGG 0.514214 1.2:-52648112 MS.gene038064:CDS
GAGACCACTCGGCGGTGGAC+CGG 0.518549 1.2:+52648311 None:intergenic
CGTTGGCCTGCCATGCCCGT+GGG 0.519717 1.2:-52648280 MS.gene038064:CDS
GCCGGGAATGGACGGGTCGT+GGG 0.520755 1.2:-52648190 MS.gene038064:CDS
ACGTTGGCCTGCCATGCCCG+TGG 0.522921 1.2:-52648281 MS.gene038064:CDS
ATCTTATCCTTTAACTCGGG+TGG 0.523502 1.2:+52647925 None:intergenic
AGTCCGTGCACTCAAAGGCT+TGG 0.523773 1.2:-52648241 MS.gene038064:CDS
GGTGGTGAAAGATCCAAGTC+CGG 0.525217 1.2:+52647943 None:intergenic
GGTCTGGAATATCCGCCGGT+GGG 0.527132 1.2:+52648354 None:intergenic
TGAGAGTACGGTGGGCCCAC+GGG 0.529134 1.2:+52648265 None:intergenic
TGGGGTCGGTCCCTCCCACC+GGG 0.530372 1.2:-52648453 MS.gene038064:CDS
ATGAGAGTACGGTGGGCCCA+CGG 0.532271 1.2:+52648264 None:intergenic
GCTTCCAAATTCCGATTCTC+CGG 0.533829 1.2:-52648330 MS.gene038064:CDS
TTCCGCAACCGGAGACTCAC+CGG 0.534590 1.2:-52648561 MS.gene038064:CDS
CGGTTGCGGAAAGTGGTTGT+CGG 0.536348 1.2:+52648573 None:intergenic
ATAATTGGTCTAATGCTCCC+TGG 0.536398 1.2:+52647155 None:intergenic
GACGAGAAGACCCGGTGGGA+GGG 0.537088 1.2:+52648443 None:intergenic
AAGAGGAATTGTTGTTGTCA+AGG 0.537371 1.2:+52648505 None:intergenic
CTTCCAAGCCTTTGAGTGCA+CGG 0.538720 1.2:+52648238 None:intergenic
TGGCATCGCCGGGAATGGAC+GGG 0.543073 1.2:-52648197 MS.gene038064:CDS
CCGGTGTCAGTGGCATCGCC+GGG 0.544222 1.2:-52648207 MS.gene038064:CDS
GGGACATAGTCAGGCGAGAA+CGG 0.547841 1.2:+52648526 None:intergenic
GTTTACAGGCTGAGAAGTGG+TGG 0.548312 1.2:-52648087 MS.gene038064:CDS
CTCATAGTCCGTGCACTCAA+AGG 0.550431 1.2:-52648246 MS.gene038064:CDS
AAATGGGTTGGCCCATAACC+CGG 0.550836 1.2:-52647962 MS.gene038064:CDS
AACGAGTTGGCGATACGGGT+CGG 0.551790 1.2:-52648161 MS.gene038064:CDS
TCAAATTCTCGCTATTTGTG+TGG 0.552114 1.2:-52647484 MS.gene038064:CDS
ACGCATGTAGGCATGTAGAT+TGG 0.554041 1.2:+52647354 None:intergenic
CTCGCTCCCTCCGACCCCAC+CGG 0.555197 1.2:-52648369 MS.gene038064:CDS
GAGTCTCCGGTTGCGGAAAG+TGG 0.555872 1.2:+52648566 None:intergenic
GTGGTGAAAGATCCAAGTCC+GGG 0.556634 1.2:+52647944 None:intergenic
AACAGAACTTAGTAGGGAAG+AGG 0.556724 1.2:+52648488 None:intergenic
AGGTGGAGACCACTCGGCGG+TGG 0.556945 1.2:+52648306 None:intergenic
CTAATGCTCCCTGGGTTGAG+AGG 0.557054 1.2:+52647164 None:intergenic
AGAGGAATTGTTGTTGTCAA+GGG 0.560702 1.2:+52648506 None:intergenic
GAAATTCTTTGCATGCCTCA+TGG 0.561075 1.2:-52647113 MS.gene038064:CDS
CCCGGCGATGCCACTGACAC+CGG 0.564808 1.2:+52648207 None:intergenic
TTACAAAACTTCTCTGGGGT+CGG 0.567115 1.2:-52648467 MS.gene038064:CDS
TCTTTGCATGCCTCATGGCA+GGG 0.567304 1.2:-52647108 MS.gene038064:CDS
GGAGTTCCAGGCGGTGCGGA+CGG 0.570604 1.2:+52648416 None:intergenic
GAGTCTTTGTTCCACAACAT+TGG 0.572372 1.2:+52648042 None:intergenic
GAGAATCGGAATTTGGAAGC+GGG 0.577136 1.2:+52648333 None:intergenic
GTCTGGAATATCCGCCGGTG+GGG 0.581278 1.2:+52648355 None:intergenic
GGAGAATCGGAATTTGGAAG+CGG 0.584387 1.2:+52648332 None:intergenic
AGCGGGTCTGGAATATCCGC+CGG 0.585759 1.2:+52648350 None:intergenic
CGATGCCACTGACACCGGTA+CGG 0.586344 1.2:+52648212 None:intergenic
GGGTCTGGAATATCCGCCGG+TGG 0.588168 1.2:+52648353 None:intergenic
CCATTCGCCTTATCCAAACT+CGG 0.588222 1.2:+52648129 None:intergenic
CCGAGTTTGGATAAGGCGAA+TGG 0.588244 1.2:-52648129 MS.gene038064:CDS
GATCCACTGTGCCAATGTTG+TGG 0.590586 1.2:-52648053 MS.gene038064:CDS
TCGTGGGAATTCAAACGAGT+TGG 0.593252 1.2:-52648174 MS.gene038064:CDS
ACGTAGAGGTGGAGACCACT+CGG 0.594306 1.2:+52648300 None:intergenic
CGAGTTAAAGGATAAGATTG+AGG 0.599820 1.2:-52647920 MS.gene038064:CDS
ACGGTTGTGACGAGAAGACC+CGG 0.609002 1.2:+52648435 None:intergenic
AGATTCCGTACCGGTGTCAG+TGG 0.611699 1.2:-52648217 MS.gene038064:CDS
GTGCACGGACTATGAGAGTA+CGG 0.611941 1.2:+52648253 None:intergenic
ATGGCAGGCCAACGTAGAGG+TGG 0.617427 1.2:+52648289 None:intergenic
TGACGAGAAGACCCGGTGGG+AGG 0.619484 1.2:+52648442 None:intergenic
ATGGGCCAACCCATTTAACC+CGG 0.621534 1.2:+52647969 None:intergenic
CCAAGAAGCATATGATAAAG+CGG 0.622342 1.2:-52647551 MS.gene038064:CDS
TAAAGGATAAGATTGAGGAA+TGG 0.623599 1.2:-52647915 MS.gene038064:CDS
GCTCCCTCCGACCCCACCGG+CGG 0.625949 1.2:-52648366 MS.gene038064:CDS
ACAAGGTGGTGTGAGGAAGA+GGG 0.626050 1.2:+52648606 None:intergenic
AGTGGTCTCCACCTCTACGT+TGG 0.627837 1.2:-52648297 MS.gene038064:CDS
AGTCAGGCGAGAACGGATGA+GGG 0.643628 1.2:+52648533 None:intergenic
GAAGTTTACAGGCTGAGAAG+TGG 0.643886 1.2:-52648090 MS.gene038064:CDS
GGCATGGCAGGCCAACGTAG+AGG 0.643969 1.2:+52648286 None:intergenic
AGGCTTGGAAGATTCCGTAC+CGG 0.644423 1.2:-52648226 MS.gene038064:CDS
TTGCGGAAAGTGGTTGTCGG+AGG 0.645779 1.2:+52648576 None:intergenic
ACGGACTATGAGAGTACGGT+GGG 0.649294 1.2:+52648257 None:intergenic
AATGACGACAAGGTGGTGTG+AGG 0.649943 1.2:+52648599 None:intergenic
GTTCCACAACATTGGCACAG+TGG 0.661542 1.2:+52648050 None:intergenic
TTTACAGGCTGAGAAGTGGT+GGG 0.664271 1.2:-52648086 MS.gene038064:CDS
TTGTCGGAGGAATGACGACA+AGG 0.674643 1.2:+52648589 None:intergenic
ACGTGGAGTTCCAGGCGGTG+CGG 0.675896 1.2:+52648412 None:intergenic
TTCTCCGGTCCACCGCCGAG+TGG 0.686008 1.2:-52648315 MS.gene038064:CDS
ATATATCCAAATGTGAACAA+TGG 0.688738 1.2:-52647883 MS.gene038064:CDS
AGACATCATGAGCTGCCACG+TGG 0.697920 1.2:+52648395 None:intergenic
CTGCCACGTGGAGTTCCAGG+CGG 0.705558 1.2:+52648407 None:intergenic
GATAGGGATTCCCTGCCATG+AGG 0.706486 1.2:+52647098 None:intergenic
CACGGACTATGAGAGTACGG+TGG 0.713455 1.2:+52648256 None:intergenic
TTGTGACGAGAAGACCCGGT+GGG 0.717453 1.2:+52648439 None:intergenic
TCTGTTACAAAACTTCTCTG+GGG 0.722297 1.2:-52648471 MS.gene038064:CDS
TCGGAGGAATGACGACAAGG+TGG 0.725756 1.2:+52648592 None:intergenic
GCACCGCCTGGAACTCCACG+TGG 0.732654 1.2:-52648410 MS.gene038064:CDS
GTTGTGACGAGAAGACCCGG+TGG 0.740905 1.2:+52648438 None:intergenic
TAGAGGTGGAGACCACTCGG+CGG 0.783474 1.2:+52648303 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTCAATTGTTTAATTC+CGG - chr1.2:52647706-52647725 MS.gene038064:intron 15.0%
!!! TTGTAATTTTCAAGAAATTA+TGG - chr1.2:52648484-52648503 MS.gene038064:CDS 15.0%
!! TAATTCAATTGTTTAATTCC+GGG - chr1.2:52647707-52647726 MS.gene038064:intron 20.0%
!!! AATTTTCAAGAAATTATGGA+TGG - chr1.2:52648488-52648507 MS.gene038064:CDS 20.0%
!!! TCATCATTGATTATTGAATT+GGG - chr1.2:52648076-52648095 MS.gene038064:CDS 20.0%
! ATATATCCAAATGTGAACAA+TGG - chr1.2:52647811-52647830 MS.gene038064:intron 25.0%
!! GTCATCATTGATTATTGAAT+TGG - chr1.2:52648075-52648094 MS.gene038064:CDS 25.0%
!! TTTTGTAACAGAACTTAGTA+GGG + chr1.2:52647215-52647234 None:intergenic 25.0%
ATACTCATACAACTTCTTCT+TGG + chr1.2:52648319-52648338 None:intergenic 30.0%
ATTACAAGTATCTGCAACTT+TGG + chr1.2:52648471-52648490 None:intergenic 30.0%
CTCAATCTTATCCTTTAACT+CGG + chr1.2:52647776-52647795 None:intergenic 30.0%
CTCATTACATACAACATCTT+TGG + chr1.2:52647677-52647696 None:intergenic 30.0%
GCTTTGCTCAAAAAATGTTT+AGG - chr1.2:52648381-52648400 MS.gene038064:CDS 30.0%
GTCTGTTTACTACTTTGATT+CGG - chr1.2:52648259-52648278 MS.gene038064:CDS 30.0%
TAAAGGATAAGATTGAGGAA+TGG - chr1.2:52647779-52647798 MS.gene038064:intron 30.0%
TCAATCTTATCCTTTAACTC+GGG + chr1.2:52647775-52647794 None:intergenic 30.0%
TGAAAATATTCGAGCATGTT+TGG + chr1.2:52647980-52647999 None:intergenic 30.0%
TGTTTAATTCCGGGTTAAAT+GGG - chr1.2:52647716-52647735 MS.gene038064:intron 30.0%
TTACAAGTATCTGCAACTTT+GGG + chr1.2:52648470-52648489 None:intergenic 30.0%
TTCTGTTACAAAACTTCTCT+GGG - chr1.2:52647222-52647241 MS.gene038064:CDS 30.0%
TTGTTTAATTCCGGGTTAAA+TGG - chr1.2:52647715-52647734 MS.gene038064:intron 30.0%
! GTTTTGTAACAGAACTTAGT+AGG + chr1.2:52647216-52647235 None:intergenic 30.0%
! GTTTTGTACATTAGACAAGT+TGG - chr1.2:52648176-52648195 MS.gene038064:CDS 30.0%
AATTAGTCCGAGTTTGGATA+AGG - chr1.2:52647558-52647577 MS.gene038064:CDS 35.0%
ACGCTGAGTTAAACAAAATC+AGG + chr1.2:52647953-52647972 None:intergenic 35.0%
ATGATGAATGTCTACTCCAA+TGG + chr1.2:52648042-52648061 None:intergenic 35.0%
ATGTGAACAATGGTGTGTAT+AGG - chr1.2:52647821-52647840 MS.gene038064:intron 35.0%
ATTGTTGCAATTTGCATTGC+AGG - chr1.2:52648103-52648122 MS.gene038064:CDS 35.0%
CGAGTTAAAGGATAAGATTG+AGG - chr1.2:52647774-52647793 MS.gene038064:intron 35.0%
CGATGAGTTTGAGAAAATCA+TGG - chr1.2:52647882-52647901 MS.gene038064:CDS 35.0%
GTTCTGTTACAAAACTTCTC+TGG - chr1.2:52647221-52647240 MS.gene038064:CDS 35.0%
TACACACCATTGTTCACATT+TGG + chr1.2:52647820-52647839 None:intergenic 35.0%
TCAAATTCTCGCTATTTGTG+TGG - chr1.2:52648210-52648229 MS.gene038064:CDS 35.0%
TCTGTTACAAAACTTCTCTG+GGG - chr1.2:52647223-52647242 MS.gene038064:CDS 35.0%
! AAGAGGAATTGTTGTTGTCA+AGG + chr1.2:52647192-52647211 None:intergenic 35.0%
! AGACTTTGAAGGAAGTTTAC+AGG - chr1.2:52647593-52647612 MS.gene038064:intron 35.0%
! AGAGGAATTGTTGTTGTCAA+GGG + chr1.2:52647191-52647210 None:intergenic 35.0%
! CCAAGAAGCATATGATAAAG+CGG - chr1.2:52648143-52648162 MS.gene038064:CDS 35.0%
! GAAAATCATGGTGACACTTT+TGG - chr1.2:52647894-52647913 MS.gene038064:CDS 35.0%
! TCTGAAGTTGAAGGCATAAT+TGG + chr1.2:52648557-52648576 None:intergenic 35.0%
!! ATTTCATGCTCTGAAGTTGA+AGG + chr1.2:52648566-52648585 None:intergenic 35.0%
!! TGGTTCAGTTGATGATGATA+GGG + chr1.2:52648615-52648634 None:intergenic 35.0%
AACAGAACTTAGTAGGGAAG+AGG + chr1.2:52647209-52647228 None:intergenic 40.0%
ATAGTATGTGTTTCGAGCCT+TGG - chr1.2:52648437-52648456 MS.gene038064:CDS 40.0%
ATCTTATCCTTTAACTCGGG+TGG + chr1.2:52647772-52647791 None:intergenic 40.0%
ATGCTTCCAACTCATACCAT+TGG - chr1.2:52648023-52648042 MS.gene038064:CDS 40.0%
CCGCTTTATCATATGCTTCT+TGG + chr1.2:52648146-52648165 None:intergenic 40.0%
CTACATGCGTGACATTTATC+AGG - chr1.2:52648353-52648372 MS.gene038064:CDS 40.0%
CTGATAAATGTCACGCATGT+AGG + chr1.2:52648355-52648374 None:intergenic 40.0%
GAAATTCTTTGCATGCCTCA+TGG - chr1.2:52648581-52648600 MS.gene038064:CDS 40.0%
GAATGGTTGCAAGACTTTGA+AGG - chr1.2:52647582-52647601 MS.gene038064:intron 40.0%
GAGTCTTTGTTCCACAACAT+TGG + chr1.2:52647655-52647674 None:intergenic 40.0%
TAATTCCGGGTTAAATGGGT+TGG - chr1.2:52647720-52647739 MS.gene038064:intron 40.0%
TCTACTCCAATGGTATGAGT+TGG + chr1.2:52648032-52648051 None:intergenic 40.0%
! ATAATTGGTCTAATGCTCCC+TGG + chr1.2:52648542-52648561 None:intergenic 40.0%
! ATTCAAACGAGTTGGCGATA+CGG - chr1.2:52647528-52647547 MS.gene038064:CDS 40.0%
! TAATTGGTCTAATGCTCCCT+GGG + chr1.2:52648541-52648560 None:intergenic 40.0%
!! ATGAGTTGGAAGCATCCAAA+GGG + chr1.2:52648018-52648037 None:intergenic 40.0%
!! CTGGTTCAGTTGATGATGAT+AGG + chr1.2:52648616-52648635 None:intergenic 40.0%
!! TATGAGTTGGAAGCATCCAA+AGG + chr1.2:52648019-52648038 None:intergenic 40.0%
!! TTACAAAACTTCTCTGGGGT+CGG - chr1.2:52647227-52647246 MS.gene038064:CDS 40.0%
AAAGATCCAAGTCCGGGTTA+TGG + chr1.2:52647747-52647766 None:intergenic 45.0%
AAGATCCAAGTCCGGGTTAT+GGG + chr1.2:52647746-52647765 None:intergenic 45.0%
ACAACAGTTAGACCTCCCTT+TGG - chr1.2:52648000-52648019 MS.gene038064:CDS 45.0%
ACGCATGTAGGCATGTAGAT+TGG + chr1.2:52648343-52648362 None:intergenic 45.0%
CCATTCGCCTTATCCAAACT+CGG + chr1.2:52647568-52647587 None:intergenic 45.0%
GAAGTTTACAGGCTGAGAAG+TGG - chr1.2:52647604-52647623 MS.gene038064:intron 45.0%
GAGAATCGGAATTTGGAAGC+GGG + chr1.2:52647364-52647383 None:intergenic 45.0%
GCAATTTGCATTGCAGGTGT+GGG - chr1.2:52648109-52648128 MS.gene038064:CDS 45.0%
GCTTCCAAATTCCGATTCTC+CGG - chr1.2:52647364-52647383 MS.gene038064:CDS 45.0%
GGAGAATCGGAATTTGGAAG+CGG + chr1.2:52647365-52647384 None:intergenic 45.0%
TCGTGGGAATTCAAACGAGT+TGG - chr1.2:52647520-52647539 MS.gene038064:CDS 45.0%
TCTTTCACCACCCGAGTTAA+AGG - chr1.2:52647762-52647781 MS.gene038064:intron 45.0%
TGCAATTTGCATTGCAGGTG+TGG - chr1.2:52648108-52648127 MS.gene038064:CDS 45.0%
TGTTGTCAAGGGACATAGTC+AGG + chr1.2:52647180-52647199 None:intergenic 45.0%
TTTACAGGCTGAGAAGTGGT+GGG - chr1.2:52647608-52647627 MS.gene038064:intron 45.0%
! CAACTTTGGGAATCTGTCCA+AGG + chr1.2:52648457-52648476 None:intergenic 45.0%
! TTCAAACGAGTTGGCGATAC+GGG - chr1.2:52647529-52647548 MS.gene038064:CDS 45.0%
AATGACGACAAGGTGGTGTG+AGG + chr1.2:52647098-52647117 None:intergenic 50.0%
ACAAGGTGGTGTGAGGAAGA+GGG + chr1.2:52647091-52647110 None:intergenic 50.0%
AGAACGGATGAGGGAACTTC+CGG + chr1.2:52647155-52647174 None:intergenic 50.0%
AGGCTTGGAAGATTCCGTAC+CGG - chr1.2:52647468-52647487 MS.gene038064:CDS 50.0%
ATGGGCCAACCCATTTAACC+CGG + chr1.2:52647728-52647747 None:intergenic 50.0%
CCGAGTTTGGATAAGGCGAA+TGG - chr1.2:52647565-52647584 MS.gene038064:CDS 50.0%
CTCATAGTCCGTGCACTCAA+AGG - chr1.2:52647448-52647467 MS.gene038064:CDS 50.0%
CTTCCAAGCCTTTGAGTGCA+CGG + chr1.2:52647459-52647478 None:intergenic 50.0%
GATCCACTGTGCCAATGTTG+TGG - chr1.2:52647641-52647660 MS.gene038064:intron 50.0%
GGTGGTGAAAGATCCAAGTC+CGG + chr1.2:52647754-52647773 None:intergenic 50.0%
GTGGTGAAAGATCCAAGTCC+GGG + chr1.2:52647753-52647772 None:intergenic 50.0%
GTTCCACAACATTGGCACAG+TGG + chr1.2:52647647-52647666 None:intergenic 50.0%
GTTTACAGGCTGAGAAGTGG+TGG - chr1.2:52647607-52647626 MS.gene038064:intron 50.0%
TCGGGCAATTAGTCCGAGTT+TGG - chr1.2:52647552-52647571 MS.gene038064:CDS 50.0%
TCTTTGCATGCCTCATGGCA+GGG - chr1.2:52648586-52648605 MS.gene038064:CDS 50.0%
TGGACCGGAGAATCGGAATT+TGG + chr1.2:52647371-52647390 None:intergenic 50.0%
TTCTTTGCATGCCTCATGGC+AGG - chr1.2:52648585-52648604 MS.gene038064:CDS 50.0%
TTGTCGGAGGAATGACGACA+AGG + chr1.2:52647108-52647127 None:intergenic 50.0%
! ACCCTTTTTCCTCTCAACCC+AGG - chr1.2:52648521-52648540 MS.gene038064:CDS 50.0%
! ACGGACTATGAGAGTACGGT+GGG + chr1.2:52647440-52647459 None:intergenic 50.0%
! CCCTGGGTTGAGAGGAAAAA+GGG + chr1.2:52648525-52648544 None:intergenic 50.0%
! CCCTTTTTCCTCTCAACCCA+GGG - chr1.2:52648522-52648541 MS.gene038064:CDS 50.0%
! GTGCACGGACTATGAGAGTA+CGG + chr1.2:52647444-52647463 None:intergenic 50.0%
! TCCCTGGGTTGAGAGGAAAA+AGG + chr1.2:52648526-52648545 None:intergenic 50.0%
!! AAATGGGTTGGCCCATAACC+CGG - chr1.2:52647732-52647751 MS.gene038064:intron 50.0%
!! AGTTGGAAGCATCCAAAGGG+AGG + chr1.2:52648015-52648034 None:intergenic 50.0%
!! CTGAGAAGTGGTGGGTATGA+TGG - chr1.2:52647616-52647635 MS.gene038064:intron 50.0%
ACGGTTGTGACGAGAAGACC+CGG + chr1.2:52647262-52647281 None:intergenic 55.0%
ACGTAGAGGTGGAGACCACT+CGG + chr1.2:52647397-52647416 None:intergenic 55.0%
AGACATCATGAGCTGCCACG+TGG + chr1.2:52647302-52647321 None:intergenic 55.0%
AGTCAGGCGAGAACGGATGA+GGG + chr1.2:52647164-52647183 None:intergenic 55.0%
AGTCCGTGCACTCAAAGGCT+TGG - chr1.2:52647453-52647472 MS.gene038064:CDS 55.0%
AGTGGTCTCCACCTCTACGT+TGG - chr1.2:52647397-52647416 MS.gene038064:CDS 55.0%
CGACAACCACTTTCCGCAAC+CGG - chr1.2:52647122-52647141 MS.gene038064:CDS 55.0%
CGGTTGCGGAAAGTGGTTGT+CGG + chr1.2:52647124-52647143 None:intergenic 55.0%
GACAAGGTGGTGTGAGGAAG+AGG + chr1.2:52647092-52647111 None:intergenic 55.0%
GATAGGGATTCCCTGCCATG+AGG + chr1.2:52648599-52648618 None:intergenic 55.0%
GGGACATAGTCAGGCGAGAA+CGG + chr1.2:52647171-52647190 None:intergenic 55.0%
TAGTCAGGCGAGAACGGATG+AGG + chr1.2:52647165-52647184 None:intergenic 55.0%
TCGGAATTTGGAAGCGGGTC+TGG + chr1.2:52647359-52647378 None:intergenic 55.0%
TCGGAGGAATGACGACAAGG+TGG + chr1.2:52647105-52647124 None:intergenic 55.0%
TTGCGGAAAGTGGTTGTCGG+AGG + chr1.2:52647121-52647140 None:intergenic 55.0%
TTGTGACGAGAAGACCCGGT+GGG + chr1.2:52647258-52647277 None:intergenic 55.0%
! AACGAGTTGGCGATACGGGT+CGG - chr1.2:52647533-52647552 MS.gene038064:CDS 55.0%
! AGATTCCGTACCGGTGTCAG+TGG - chr1.2:52647477-52647496 MS.gene038064:CDS 55.0%
! CACGGACTATGAGAGTACGG+TGG + chr1.2:52647441-52647460 None:intergenic 55.0%
! CTAATGCTCCCTGGGTTGAG+AGG + chr1.2:52648533-52648552 None:intergenic 55.0%
ATGGCAGGCCAACGTAGAGG+TGG + chr1.2:52647408-52647427 None:intergenic 60.0%
CGATGCCACTGACACCGGTA+CGG + chr1.2:52647485-52647504 None:intergenic 60.0%
GAGTCTCCGGTTGCGGAAAG+TGG + chr1.2:52647131-52647150 None:intergenic 60.0%
GGGAACTTCCGGTGAGTCTC+CGG + chr1.2:52647144-52647163 None:intergenic 60.0%
GTTGGCCCATAACCCGGACT+TGG - chr1.2:52647738-52647757 MS.gene038064:intron 60.0%
GTTGTGACGAGAAGACCCGG+TGG + chr1.2:52647259-52647278 None:intergenic 60.0%
TAGAGGTGGAGACCACTCGG+CGG + chr1.2:52647394-52647413 None:intergenic 60.0%
TTCCGCAACCGGAGACTCAC+CGG - chr1.2:52647133-52647152 MS.gene038064:CDS 60.0%
TTCCGGTGAGTCTCCGGTTG+CGG + chr1.2:52647138-52647157 None:intergenic 60.0%
! ACGAGTTGGCGATACGGGTC+GGG - chr1.2:52647534-52647553 MS.gene038064:CDS 60.0%
! AGCGGGTCTGGAATATCCGC+CGG + chr1.2:52647347-52647366 None:intergenic 60.0%
! ATGAGAGTACGGTGGGCCCA+CGG + chr1.2:52647433-52647452 None:intergenic 60.0%
! GGTCTGGAATATCCGCCGGT+GGG + chr1.2:52647343-52647362 None:intergenic 60.0%
! GTCTGGAATATCCGCCGGTG+GGG + chr1.2:52647342-52647361 None:intergenic 60.0%
ACGTGGAGTTCCAGGCGGTG+CGG + chr1.2:52647285-52647304 None:intergenic 65.0%
CTGCCACGTGGAGTTCCAGG+CGG + chr1.2:52647290-52647309 None:intergenic 65.0%
GACGAGAAGACCCGGTGGGA+GGG + chr1.2:52647254-52647273 None:intergenic 65.0%
GAGCTGCCACGTGGAGTTCC+AGG + chr1.2:52647293-52647312 None:intergenic 65.0%
GGAATATCCGCCGGTGGGGT+CGG + chr1.2:52647338-52647357 None:intergenic 65.0%
GGCATGGCAGGCCAACGTAG+AGG + chr1.2:52647411-52647430 None:intergenic 65.0%
GTCAGTGGCATCGCCGGGAA+TGG - chr1.2:52647492-52647511 MS.gene038064:CDS 65.0%
GTGGCATCGCCGGGAATGGA+CGG - chr1.2:52647496-52647515 MS.gene038064:CDS 65.0%
TCCCACGACCCGTCCATTCC+CGG + chr1.2:52647508-52647527 None:intergenic 65.0%
TCGGCGGTGGACCGGAGAAT+CGG + chr1.2:52647378-52647397 None:intergenic 65.0%
TGACGAGAAGACCCGGTGGG+AGG + chr1.2:52647255-52647274 None:intergenic 65.0%
TGGCATCGCCGGGAATGGAC+GGG - chr1.2:52647497-52647516 MS.gene038064:CDS 65.0%
! ACCGGTGTCAGTGGCATCGC+CGG - chr1.2:52647486-52647505 MS.gene038064:CDS 65.0%
! GGGTCTGGAATATCCGCCGG+TGG + chr1.2:52647344-52647363 None:intergenic 65.0%
! TGAGAGTACGGTGGGCCCAC+GGG + chr1.2:52647432-52647451 None:intergenic 65.0%
AGGTGGAGACCACTCGGCGG+TGG + chr1.2:52647391-52647410 None:intergenic 70.0%
ATATCCGCCGGTGGGGTCGG+AGG + chr1.2:52647335-52647354 None:intergenic 70.0%
CCCGGCGATGCCACTGACAC+CGG + chr1.2:52647490-52647509 None:intergenic 70.0%
GAGACCACTCGGCGGTGGAC+CGG + chr1.2:52647386-52647405 None:intergenic 70.0%
GCACCGCCTGGAACTCCACG+TGG - chr1.2:52647284-52647303 MS.gene038064:intron 70.0%
GCCGGGAATGGACGGGTCGT+GGG - chr1.2:52647504-52647523 MS.gene038064:CDS 70.0%
GGAGTTCCAGGCGGTGCGGA+CGG + chr1.2:52647281-52647300 None:intergenic 70.0%
TATCCGCCGGTGGGGTCGGA+GGG + chr1.2:52647334-52647353 None:intergenic 70.0%
TCACAACCGTCCGCACCGCC+TGG - chr1.2:52647272-52647291 MS.gene038064:intron 70.0%
TTCTCCGGTCCACCGCCGAG+TGG - chr1.2:52647379-52647398 MS.gene038064:CDS 70.0%
! ACGTTGGCCTGCCATGCCCG+TGG - chr1.2:52647413-52647432 MS.gene038064:CDS 70.0%
! CCGGTGTCAGTGGCATCGCC+GGG - chr1.2:52647487-52647506 MS.gene038064:CDS 70.0%
! CGTTGGCCTGCCATGCCCGT+GGG - chr1.2:52647414-52647433 MS.gene038064:CDS 70.0%
CGCCGGGAATGGACGGGTCG+TGG - chr1.2:52647503-52647522 MS.gene038064:CDS 75.0%
CTCGCTCCCTCCGACCCCAC+CGG - chr1.2:52647325-52647344 MS.gene038064:intron 75.0%
CTGGGGTCGGTCCCTCCCAC+CGG - chr1.2:52647240-52647259 MS.gene038064:intron 75.0%
GTACGGTGGGCCCACGGGCA+TGG + chr1.2:52647427-52647446 None:intergenic 75.0%
TGGGGTCGGTCCCTCCCACC+GGG - chr1.2:52647241-52647260 MS.gene038064:intron 75.0%
! GCTCCCTCCGACCCCACCGG+CGG - chr1.2:52647328-52647347 MS.gene038064:intron 80.0%
! GGTGGGCCCACGGGCATGGC+AGG + chr1.2:52647423-52647442 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 52647067 52648649 52647067 ID=MS.gene038064
chr1.2 mRNA 52647067 52648649 52647067 ID=MS.gene038064.t1;Parent=MS.gene038064
chr1.2 exon 52647874 52648649 52647874 ID=MS.gene038064.t1.exon1;Parent=MS.gene038064.t1
chr1.2 CDS 52647874 52648649 52647874 ID=cds.MS.gene038064.t1;Parent=MS.gene038064.t1
chr1.2 exon 52647342 52647591 52647342 ID=MS.gene038064.t1.exon2;Parent=MS.gene038064.t1
chr1.2 CDS 52647342 52647591 52647342 ID=cds.MS.gene038064.t1;Parent=MS.gene038064.t1
chr1.2 exon 52647067 52647252 52647067 ID=MS.gene038064.t1.exon3;Parent=MS.gene038064.t1
chr1.2 CDS 52647067 52647252 52647067 ID=cds.MS.gene038064.t1;Parent=MS.gene038064.t1
Gene Sequence

>MS.gene038064

ATGTCGATGTTGTGCGCTTCTCCCTCTTCCTCACACCACCTTGTCGTCATTCCTCCGACAACCACTTTCCGCAACCGGAGACTCACCGGAAGTTCCCTCATCCGTTCTCGCCTGACTATGTCCCTTGACAACAACAATTCCTCTTCCCTACTAAGTTCTGTTACAAAACTTCTCTGGGGTCGGTCCCTCCCACCGGGTCTTCTCGTCACAACCGTCCGCACCGCCTGGAACTCCACGTGGCAGCTCATGATGTCTCAGCTCGCTCCCTCCGACCCCACCGGCGGATATTCCAGACCCGCTTCCAAATTCCGATTCTCCGGTCCACCGCCGAGTGGTCTCCACCTCTACGTTGGCCTGCCATGCCCGTGGGCCCACCGTACTCTCATAGTCCGTGCACTCAAAGGCTTGGAAGATTCCGTACCGGTGTCAGTGGCATCGCCGGGAATGGACGGGTCGTGGGAATTCAAACGAGTTGGCGATACGGGTCGGGCAATTAGTCCGAGTTTGGATAAGGCGAATGGTTGCAAGACTTTGAAGGAAGTTTACAGGCTGAGAAGTGGTGGGTATGATGGAAGATCCACTGTGCCAATGTTGTGGAACAAAGACTCCAAAGATGTTGTATGTAATGAGAGTTATGATATAATTCAATTGTTTAATTCCGGGTTAAATGGGTTGGCCCATAACCCGGACTTGGATCTTTCACCACCCGAGTTAAAGGATAAGATTGAGGAATGGTGTCAAGTGATATATCCAAATGTGAACAATGGTGTGTATAGGTAAGAAATGCTAACAACTGCGTGCAACTTCTGTAATCACGATGAGTTTGAGAAAATCATGGTGACACTTTTGGTTTTGTTCAAAGCATATCATTGTAGCAAGTCATCCTGATTTTGTTTAACTCAGCGTGATTCCAAACATGCTCGAATATTTTCAACAACAGTTAGACCTCCCTTTGGATGCTTCCAACTCATACCATTGGAGTAGACATTCATCATTAATTTTGCTTCTGTCATCATTGATTATTGAATTGGGTATTATTGTTGCAATTTGCATTGCAGGTGTGGGTTTGCACAGAGCCAAGAAGCATATGATAAAGCGGTGAATGAGTTGTTTTGTACATTAGACAAGTTGGAGAATCACTTATCAAATTCTCGCTATTTGTGTGGAGACGCGCTCACACTTGTTGATATATGTCTGTTTACTACTTTGATTCGGTTTGATATTGCTTATAATGTTTTGTTTAAATGCACCAAGAAGAAGTTGTATGAGTATACCAATCTACATGCCTACATGCGTGACATTTATCAGGTGCTTGCTTTGCTCAAAAAATGTTTAGGTTTATCTATCATTTGTGTTGTTTGTCTACTAACATAGTATGTGTTTCGAGCCTTGGACAGATTCCCAAAGTTGCAGATACTTGTAATTTTCAAGAAATTATGGATGGTTACTACAAAACCCTTTTTCCTCTCAACCCAGGGAGCATTAGACCAATTATGCCTTCAACTTCAGAGCATGAAATTCTTTGCATGCCTCATGGCAGGGAATCCCTATCATCATCAACTGAACCAGTATTTGTTAAATAG
Protein sequence

>MS.gene038064.t1

MSMLCASPSSSHHLVVIPPTTTFRNRRLTGSSLIRSRLTMSLDNNNSSSLLSSVTKLLWGRSLPPGLLVTTVRTAWNSTWQLMMSQLAPSDPTGGYSRPASKFRFSGPPPSGLHLYVGLPCPWAHRTLIVRALKGLEDSVPVSVASPGMDGSWEFKRVGDTGRAISPSLDKANGCKTLKEVYRLRSGGYDGRSTVPMLWNKDSKDVVCNESYDIIQLFNSGLNGLAHNPDLDLSPPELKDKIEEWCQVIYPNVNNGVYRCGFAQSQEAYDKAVNELFCTLDKLENHLSNSRYLCGDALTLVDICLFTTLIRFDIAYNVLFKCTKKKLYEYTNLHAYMRDIYQIPKVADTCNFQEIMDGYYKTLFPLNPGSIRPIMPSTSEHEILCMPHGRESLSSSTEPVFVK