AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006897

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006897.t1 MTR_4g084040 99.077 325 3 0 1 325 31 355 0.0 674
MS.gene006897.t1 MTR_4g084040 99.077 325 3 0 1 325 1 325 0.0 671
MS.gene006897.t1 MTR_4g084040 98.824 255 3 0 71 325 5 259 0.0 527
MS.gene006897.t1 MTR_1g069575 42.598 331 167 6 1 325 86 399 4.35e-80 249
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006897.t1 AT4G19880 83.025 324 55 0 1 324 32 355 0.0 578
MS.gene006897.t1 AT4G19880 76.857 350 55 1 1 324 32 381 0.0 566
MS.gene006897.t1 AT5G45020 77.538 325 73 0 1 325 1 325 0.0 552
MS.gene006897.t1 AT5G45020 77.538 325 73 0 1 325 1 325 0.0 552
MS.gene006897.t1 AT5G44990 74.516 310 79 0 15 324 12 321 1.21e-179 500
MS.gene006897.t1 AT5G44990 74.516 310 79 0 15 324 12 321 1.21e-179 500
MS.gene006897.t1 AT4G19880 81.526 249 46 0 1 249 32 280 5.24e-154 434
MS.gene006897.t1 AT5G44990 76.596 235 55 0 15 249 12 246 3.31e-137 390
MS.gene006897.t1 AT5G44990 73.109 238 64 0 87 324 1 238 7.78e-132 376
MS.gene006897.t1 AT5G44000 40.615 325 182 3 1 321 70 387 5.02e-78 244

Find 74 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCATTAGTCTCATCAATT+TGG 0.137715 4.2:-25894025 None:intergenic
TCAGCTGGAAATTGGGAATT+TGG 0.189302 4.2:-25893392 None:intergenic
TTCCTGACTCAGCTGGAAAT+TGG 0.212468 4.2:-25893400 None:intergenic
ATCAGTCGGTCAAACCTATA+TGG 0.237836 4.2:+25893610 MS.gene006897:intron
TAAACACCGTTGTTTATATT+AGG 0.242592 4.2:-25894056 None:intergenic
TTGGTGTAATTGAAGATATT+TGG 0.251559 4.2:-25895224 None:intergenic
TAAAGAATCAGATGAATATA+TGG 0.262799 4.2:+25893641 MS.gene006897:CDS
CTCATTAGTCTCATCAATTT+GGG 0.279709 4.2:-25894024 None:intergenic
TACTGCTAATGCCAGGGATT+TGG 0.306689 4.2:-25895256 None:intergenic
TACAGGAACAGTGAACTTTC+CGG 0.323413 4.2:-25893772 None:intergenic
CATCCTTCTATCAATCCATT+TGG 0.329006 4.2:+25895320 MS.gene006897:CDS
CATATAAAGTTGCATTACTA+TGG 0.346462 4.2:+25895293 MS.gene006897:CDS
AGGACCTCGGTATTCGATTC+AGG 0.356321 4.2:-25893675 None:intergenic
GGAGCAAAATAATCGATATT+TGG 0.360002 4.2:-25895359 None:intergenic
TCAGTCGGTCAAACCTATAT+GGG 0.381315 4.2:+25893611 MS.gene006897:intron
TCCATTTGGGATCGTTCCTA+TGG 0.386731 4.2:+25895334 MS.gene006897:CDS
AGGGATTTGGAAAATGTCTT+TGG 0.388256 4.2:-25895243 None:intergenic
TCCTGACTCAGCTGGAAATT+GGG 0.392249 4.2:-25893399 None:intergenic
TGACAAAGCCATCAGTTTCT+CGG 0.393121 4.2:+25893496 MS.gene006897:CDS
ACATATCATATGCTTGTCCT+TGG 0.402858 4.2:+25893435 MS.gene006897:CDS
TCTTTAGTTCTTCCCCATAT+AGG 0.406552 4.2:-25893624 None:intergenic
ATCCTTCTATCAATCCATTT+GGG 0.416102 4.2:+25895321 MS.gene006897:CDS
TTTGCTCCATTCAAAGGATC+AGG 0.416369 4.2:-25893708 None:intergenic
CATATCATATGCTTGTCCTT+GGG 0.421419 4.2:+25893436 MS.gene006897:CDS
AGTGTAACAAGAAGCTAATA+CGG 0.426098 4.2:+25895195 MS.gene006897:CDS
TGAAATGGTGCGATCATCAT+TGG 0.429670 4.2:+25893301 None:intergenic
TCCCAATTTCCAGCTGAGTC+AGG 0.435802 4.2:+25893398 MS.gene006897:CDS
TTGCTCCTCACGACAGAGAA+AGG 0.440332 4.2:+25895375 MS.gene006897:CDS
ATCCCAAATGGATTGATAGA+AGG 0.448428 4.2:-25895323 None:intergenic
AAGAGAATTATATGATGCTT+TGG 0.452830 4.2:+25894219 MS.gene006897:CDS
TGATATCTTCCTGACTCAGC+TGG 0.462761 4.2:-25893407 None:intergenic
TTCACTGTACTGCTAATGCC+AGG 0.471207 4.2:-25895263 None:intergenic
CCATTTGGGATCGTTCCTAT+GGG 0.473199 4.2:+25895335 MS.gene006897:CDS
AAAGAATCAGATGAATATAT+GGG 0.478002 4.2:+25893642 MS.gene006897:CDS
GAATCGAATACCGAGGTCCT+TGG 0.488511 4.2:+25893678 MS.gene006897:CDS
TAGATACATACATCAATGTA+TGG 0.492426 4.2:-25894107 None:intergenic
AACAACGGTGTTTATAGATG+TGG 0.501716 4.2:+25894065 MS.gene006897:CDS
TAATCGATATTTGGCCCCAT+AGG 0.502361 4.2:-25895350 None:intergenic
CTTGCTTACTTGAAAATCAA+AGG 0.511306 4.2:+25893470 MS.gene006897:CDS
GCTGAACCTGATCCTTTGAA+TGG 0.511651 4.2:+25893702 MS.gene006897:CDS
TATTGCAGAAAATCCCATCT+TGG 0.515927 4.2:+25893977 MS.gene006897:CDS
ACACTCTTTGCTCCATTCAA+AGG 0.520150 4.2:-25893714 None:intergenic
GATCCTTTGAAACGAATTGT+CGG 0.522587 4.2:-25893370 None:intergenic
GTTCCGACAATTCGTTTCAA+AGG 0.532082 4.2:+25893367 MS.gene006897:CDS
CCCATAGGAACGATCCCAAA+TGG 0.534040 4.2:-25895335 None:intergenic
ACAACGGTGTTTATAGATGT+GGG 0.539714 4.2:+25894066 MS.gene006897:CDS
AGAGGGATACAAGTCCAAGA+TGG 0.539809 4.2:-25893991 None:intergenic
ATGTGGGTTTGCAAAGAAGC+AGG 0.547240 4.2:+25894082 MS.gene006897:CDS
CACTCTCATTAGATTTGATG+AGG 0.551830 4.2:+25894321 MS.gene006897:CDS
AATTTGGGCTTGCAATTCAG+AGG 0.553763 4.2:-25894009 None:intergenic
TTTGATCTGCACAGATTCTG+TGG 0.559429 4.2:+25893880 MS.gene006897:intron
CATTAGCAGTACAGTGAACA+TGG 0.560651 4.2:+25895268 MS.gene006897:CDS
TTGATCTGCACAGATTCTGT+GGG 0.561111 4.2:+25893881 MS.gene006897:intron
GAGGGATACAAGTCCAAGAT+GGG 0.566317 4.2:-25893990 None:intergenic
AATCAGATGAATATATGGGA+TGG 0.573584 4.2:+25893646 MS.gene006897:CDS
AATTGTTAACAACGAGAGCT+CGG 0.575907 4.2:+25893920 MS.gene006897:CDS
GTGTAACAAGAAGCTAATAC+GGG 0.576905 4.2:+25895196 MS.gene006897:CDS
AGCAAGCAACGCTATATATG+TGG 0.582625 4.2:+25894262 MS.gene006897:CDS
AAGGATCAGGTTCAGCTCCA+AGG 0.583231 4.2:-25893695 None:intergenic
TGAAACGAATTGTCGGAACG+TGG 0.588416 4.2:-25893363 None:intergenic
ATTTGGGCTTGCAATTCAGA+GGG 0.602774 4.2:-25894008 None:intergenic
CCTACTAACCGAGAAACTGA+TGG 0.608850 4.2:-25893504 None:intergenic
ATTGGATGAGATATCAGACT+CGG 0.620767 4.2:+25893319 MS.gene006897:CDS
GCAAGGCATCTGCAAGCCCA+AGG 0.621992 4.2:-25893452 None:intergenic
TTGGATGAGATATCAGACTC+GGG 0.645525 4.2:+25893320 MS.gene006897:CDS
CAGTCGGTCAAACCTATATG+GGG 0.647505 4.2:+25893612 MS.gene006897:intron
AGGTTCAGCTCCAAGGACCT+CGG 0.647588 4.2:-25893688 None:intergenic
ATTGCGAGTACAAACTACTC+CGG 0.648781 4.2:+25893753 MS.gene006897:CDS
TGATGCTTTGGACAAATGCG+AGG 0.659801 4.2:+25894231 MS.gene006897:CDS
TCACTGTACTGCTAATGCCA+GGG 0.660442 4.2:-25895262 None:intergenic
GAAAACCTTTCTCTGTCGTG+AGG 0.664094 4.2:-25895380 None:intergenic
CATTTGGGATCGTTCCTATG+GGG 0.667700 4.2:+25895336 MS.gene006897:CDS
ATATATCCTAATATAAACAA+CGG 0.698242 4.2:+25894050 MS.gene006897:CDS
AAATTGATGAGACTAATGAG+TGG 0.709533 4.2:+25894027 MS.gene006897:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACATTAAAATTCAATA+TGG + chr4.2:25894805-25894824 MS.gene006897:intron 10.0%
!! ATATATCCTAATATAAACAA+CGG + chr4.2:25894050-25894069 MS.gene006897:CDS 15.0%
!! TTACTTAAAAATCAAAACAT+CGG - chr4.2:25895134-25895153 None:intergenic 15.0%
!!! TATCTATTTTTTACTTTTTC+AGG + chr4.2:25894124-25894143 MS.gene006897:intron 15.0%
!! AAAGAATCAGATGAATATAT+GGG + chr4.2:25893642-25893661 MS.gene006897:CDS 20.0%
!! ATAATAAAGCAATAACAAGA+AGG - chr4.2:25894905-25894924 None:intergenic 20.0%
!! TAAAGAATCAGATGAATATA+TGG + chr4.2:25893641-25893660 MS.gene006897:CDS 20.0%
!! TACTTAAAAATCAAAACATC+GGG - chr4.2:25895133-25895152 None:intergenic 20.0%
!! TTGATTTATTAATTCAGACA+AGG - chr4.2:25894417-25894436 None:intergenic 20.0%
!!! ATTGCTTTATTATTATTCGA+TGG + chr4.2:25894912-25894931 MS.gene006897:intron 20.0%
! AACAAAATGAGTCACAAAAT+AGG - chr4.2:25895097-25895116 None:intergenic 25.0%
! AAGAGAATTATATGATGCTT+TGG + chr4.2:25894219-25894238 MS.gene006897:CDS 25.0%
! AATTAATAAATCAACCTCAG+CGG + chr4.2:25894423-25894442 MS.gene006897:intron 25.0%
! CATATAAAGTTGCATTACTA+TGG + chr4.2:25895293-25895312 MS.gene006897:CDS 25.0%
! TAAACACCGTTGTTTATATT+AGG - chr4.2:25894059-25894078 None:intergenic 25.0%
! TAAACCTGCAATTATAACAA+TGG - chr4.2:25895161-25895180 None:intergenic 25.0%
! TAGATACATACATCAATGTA+TGG - chr4.2:25894110-25894129 None:intergenic 25.0%
! TTCTAATCTCATTCTATGAA+TGG - chr4.2:25894471-25894490 None:intergenic 25.0%
! TTGCATGTTTAATAATCAGT+CGG + chr4.2:25893596-25893615 MS.gene006897:intron 25.0%
! TTGGTGTAATTGAAGATATT+TGG - chr4.2:25895227-25895246 None:intergenic 25.0%
!! AGTTTTCAATAGTTCTTACT+TGG + chr4.2:25893562-25893581 MS.gene006897:intron 25.0%
!! ATATCTGTTCAACATTTTGA+TGG + chr4.2:25895034-25895053 MS.gene006897:intron 25.0%
!! TTTTGCTTAATATTTGCAAG+TGG + chr4.2:25893530-25893549 MS.gene006897:intron 25.0%
!!! GCAATTTGGTTTTTGAAATT+CGG - chr4.2:25894717-25894736 None:intergenic 25.0%
AAAAATCAAAACATCGGGAT+GGG - chr4.2:25895128-25895147 None:intergenic 30.0%
AAATTGATGAGACTAATGAG+TGG + chr4.2:25894027-25894046 MS.gene006897:CDS 30.0%
AAGGATATGTTTAGATAGAG+AGG - chr4.2:25894842-25894861 None:intergenic 30.0%
AATAACAAGAAGGAAACACT+TGG - chr4.2:25894895-25894914 None:intergenic 30.0%
AATCAGATGAATATATGGGA+TGG + chr4.2:25893646-25893665 MS.gene006897:CDS 30.0%
AGTGTAACAAGAAGCTAATA+CGG + chr4.2:25895195-25895214 MS.gene006897:CDS 30.0%
ATCCTTCTATCAATCCATTT+GGG + chr4.2:25895321-25895340 MS.gene006897:CDS 30.0%
ATGTTATGTACCAAACTGTT+TGG - chr4.2:25894627-25894646 None:intergenic 30.0%
ATTATTCGATGGACACTTAA+TGG + chr4.2:25894923-25894942 MS.gene006897:intron 30.0%
CTCTATCTAAACATATCCTT+AGG + chr4.2:25894842-25894861 MS.gene006897:intron 30.0%
CTTGCTTACTTGAAAATCAA+AGG + chr4.2:25893470-25893489 MS.gene006897:CDS 30.0%
GGAGCAAAATAATCGATATT+TGG - chr4.2:25895362-25895381 None:intergenic 30.0%
TAAAAATCAAAACATCGGGA+TGG - chr4.2:25895129-25895148 None:intergenic 30.0%
TAACTTATAGCATGCTAAAG+AGG + chr4.2:25894948-25894967 MS.gene006897:intron 30.0%
TACTCCCAAATATCAAGAAT+AGG - chr4.2:25894379-25894398 None:intergenic 30.0%
TATGTTTAGATAGAGAGGTT+TGG - chr4.2:25894837-25894856 None:intergenic 30.0%
! ACTCATTAGTCTCATCAATT+TGG - chr4.2:25894028-25894047 None:intergenic 30.0%
! AGCATTTGAAACTGTTCATT+TGG + chr4.2:25894179-25894198 MS.gene006897:intron 30.0%
! AGTTTACTGATTAATGCATG+TGG - chr4.2:25894870-25894889 None:intergenic 30.0%
! CTCATTAGTCTCATCAATTT+GGG - chr4.2:25894027-25894046 None:intergenic 30.0%
! TGCGTAACTTGTCAAAAATA+TGG + chr4.2:25894754-25894773 MS.gene006897:intron 30.0%
!! ATTCTAAAGCAAAAACCTAC+AGG - chr4.2:25893792-25893811 None:intergenic 30.0%
!! CAACATTTTGATGGAATTAG+AGG + chr4.2:25895043-25895062 MS.gene006897:intron 30.0%
!!! CTTTGATTTTCAAGTAAGCA+AGG - chr4.2:25893472-25893491 None:intergenic 30.0%
!!! TGTAGGTTTTTGCTTTAGAA+TGG + chr4.2:25893791-25893810 MS.gene006897:intron 30.0%
AACAACGGTGTTTATAGATG+TGG + chr4.2:25894065-25894084 MS.gene006897:CDS 35.0%
AATTGTTAACAACGAGAGCT+CGG + chr4.2:25893920-25893939 MS.gene006897:CDS 35.0%
ACAACGGTGTTTATAGATGT+GGG + chr4.2:25894066-25894085 MS.gene006897:CDS 35.0%
ACATATCATATGCTTGTCCT+TGG + chr4.2:25893435-25893454 MS.gene006897:CDS 35.0%
AGGGATTTGGAAAATGTCTT+TGG - chr4.2:25895246-25895265 None:intergenic 35.0%
AGTTACGCAGTCTTAAAACT+AGG - chr4.2:25894743-25894762 None:intergenic 35.0%
ATTGGATGAGATATCAGACT+CGG + chr4.2:25893319-25893338 MS.gene006897:CDS 35.0%
CACTCTCATTAGATTTGATG+AGG + chr4.2:25894321-25894340 MS.gene006897:CDS 35.0%
CATATCATATGCTTGTCCTT+GGG + chr4.2:25893436-25893455 MS.gene006897:CDS 35.0%
CATCCTTCTATCAATCCATT+TGG + chr4.2:25895320-25895339 MS.gene006897:CDS 35.0%
GGCACCTATTCTTGATATTT+GGG + chr4.2:25894372-25894391 MS.gene006897:intron 35.0%
GTGTAACAAGAAGCTAATAC+GGG + chr4.2:25895196-25895215 MS.gene006897:CDS 35.0%
GTTACGCAGTCTTAAAACTA+GGG - chr4.2:25894742-25894761 None:intergenic 35.0%
TAGAGGAACATACAAGAACT+GGG + chr4.2:25895060-25895079 MS.gene006897:intron 35.0%
TATACCAGCAAAGGTTACTA+GGG - chr4.2:25894529-25894548 None:intergenic 35.0%
TATCAAACAGTTTGAGCACT+CGG - chr4.2:25894694-25894713 None:intergenic 35.0%
TATTGCAGAAAATCCCATCT+TGG + chr4.2:25893977-25893996 MS.gene006897:CDS 35.0%
TTACGCAGTCTTAAAACTAG+GGG - chr4.2:25894741-25894760 None:intergenic 35.0%
TTAGAGGAACATACAAGAAC+TGG + chr4.2:25895059-25895078 MS.gene006897:intron 35.0%
TTCGCCATTGTTATAATTGC+AGG + chr4.2:25895154-25895173 MS.gene006897:intron 35.0%
! AAAGACATTTTCCAAATCCC+TGG + chr4.2:25895245-25895264 MS.gene006897:CDS 35.0%
! TTAAAACTAGGGGTGCAATT+TGG - chr4.2:25894731-25894750 None:intergenic 35.0%
!! ATCCCAAATGGATTGATAGA+AGG - chr4.2:25895326-25895345 None:intergenic 35.0%
!! GATCCTTTGAAACGAATTGT+CGG - chr4.2:25893373-25893392 None:intergenic 35.0%
!! TAAAGAGGAAAGCTTTGAAG+TGG + chr4.2:25894963-25894982 MS.gene006897:intron 35.0%
!! TCTTTAGTTCTTCCCCATAT+AGG - chr4.2:25893627-25893646 None:intergenic 35.0%
AATTTGGGCTTGCAATTCAG+AGG - chr4.2:25894012-25894031 None:intergenic 40.0%
ACACTCTTTGCTCCATTCAA+AGG - chr4.2:25893717-25893736 None:intergenic 40.0%
AGAGGAACATACAAGAACTG+GGG + chr4.2:25895061-25895080 MS.gene006897:intron 40.0%
AGCAAGCAACGCTATATATG+TGG + chr4.2:25894262-25894281 MS.gene006897:CDS 40.0%
ATCAGTCGGTCAAACCTATA+TGG + chr4.2:25893610-25893629 MS.gene006897:intron 40.0%
ATGACCAAGTATACCAGCAA+AGG - chr4.2:25894538-25894557 None:intergenic 40.0%
ATTAATGCATGTGGTGCCTA+AGG - chr4.2:25894861-25894880 None:intergenic 40.0%
ATTGCGAGTACAAACTACTC+CGG + chr4.2:25893753-25893772 MS.gene006897:CDS 40.0%
ATTTGGGCTTGCAATTCAGA+GGG - chr4.2:25894011-25894030 None:intergenic 40.0%
CATTAGCAGTACAGTGAACA+TGG + chr4.2:25895268-25895287 MS.gene006897:CDS 40.0%
GGGCACCTATTCTTGATATT+TGG + chr4.2:25894371-25894390 MS.gene006897:intron 40.0%
GTAACCTTTGCTGGTATACT+TGG + chr4.2:25894531-25894550 MS.gene006897:intron 40.0%
GTATACCAGCAAAGGTTACT+AGG - chr4.2:25894530-25894549 None:intergenic 40.0%
GTTCCGACAATTCGTTTCAA+AGG + chr4.2:25893367-25893386 MS.gene006897:CDS 40.0%
TAATAAATCAACCTCAGCGG+AGG + chr4.2:25894426-25894445 MS.gene006897:intron 40.0%
TAATCGATATTTGGCCCCAT+AGG - chr4.2:25895353-25895372 None:intergenic 40.0%
TACAGGAACAGTGAACTTTC+CGG - chr4.2:25893775-25893794 None:intergenic 40.0%
TAGATAGAGAGGTTTGGAGA+GGG - chr4.2:25894831-25894850 None:intergenic 40.0%
TCAGCTGGAAATTGGGAATT+TGG - chr4.2:25893395-25893414 None:intergenic 40.0%
TCAGTCGGTCAAACCTATAT+GGG + chr4.2:25893611-25893630 MS.gene006897:intron 40.0%
TGACAAAGCCATCAGTTTCT+CGG + chr4.2:25893496-25893515 MS.gene006897:CDS 40.0%
TTAGATAGAGAGGTTTGGAG+AGG - chr4.2:25894832-25894851 None:intergenic 40.0%
TTGATCTGCACAGATTCTGT+GGG + chr4.2:25893881-25893900 MS.gene006897:intron 40.0%
TTGGATGAGATATCAGACTC+GGG + chr4.2:25893320-25893339 MS.gene006897:CDS 40.0%
TTTGATCTGCACAGATTCTG+TGG + chr4.2:25893880-25893899 MS.gene006897:intron 40.0%
TTTGCTCCATTCAAAGGATC+AGG - chr4.2:25893711-25893730 None:intergenic 40.0%
! TTTTCCTGAATCGAATACCG+AGG + chr4.2:25893671-25893690 MS.gene006897:CDS 40.0%
ACTCAATCCTCTGATGGAGT+CGG - chr4.2:25894496-25894515 None:intergenic 45.0%
AGAGGGATACAAGTCCAAGA+TGG - chr4.2:25893994-25894013 None:intergenic 45.0%
AGTACCCTAGTAACCTTTGC+TGG + chr4.2:25894522-25894541 MS.gene006897:intron 45.0%
ATAGTGTGCGACTTTGTGTG+TGG + chr4.2:25894345-25894364 MS.gene006897:intron 45.0%
ATGTGGGTTTGCAAAGAAGC+AGG + chr4.2:25894082-25894101 MS.gene006897:CDS 45.0%
CAGTCGGTCAAACCTATATG+GGG + chr4.2:25893612-25893631 MS.gene006897:intron 45.0%
CCATCAGTTTCTCGGTTAGT+AGG + chr4.2:25893504-25893523 MS.gene006897:intron 45.0%
CCTAAAAGGCGAAGACATTC+AGG + chr4.2:25894664-25894683 MS.gene006897:intron 45.0%
CCTACTAACCGAGAAACTGA+TGG - chr4.2:25893507-25893526 None:intergenic 45.0%
CGTTCAACTCAATCCTCTGA+TGG - chr4.2:25894502-25894521 None:intergenic 45.0%
GAAAACCTTTCTCTGTCGTG+AGG - chr4.2:25895383-25895402 None:intergenic 45.0%
GACTGAGTTGCCAAACAGTT+TGG + chr4.2:25894614-25894633 MS.gene006897:intron 45.0%
GAGGGATACAAGTCCAAGAT+GGG - chr4.2:25893993-25894012 None:intergenic 45.0%
GCGTTAGGTCTTAAAGGTGA+AGG + chr4.2:25894581-25894600 MS.gene006897:intron 45.0%
GCTGAACCTGATCCTTTGAA+TGG + chr4.2:25893702-25893721 MS.gene006897:CDS 45.0%
GGAAAGTTCACTGTTCCTGT+AGG + chr4.2:25893774-25893793 MS.gene006897:intron 45.0%
GTTCATTTGGTCCTTTGTGC+AGG + chr4.2:25894192-25894211 MS.gene006897:intron 45.0%
TACTGCTAATGCCAGGGATT+TGG - chr4.2:25895259-25895278 None:intergenic 45.0%
TAGTGTGCGACTTTGTGTGT+GGG + chr4.2:25894346-25894365 MS.gene006897:intron 45.0%
TCACTGTACTGCTAATGCCA+GGG - chr4.2:25895265-25895284 None:intergenic 45.0%
TCCTGACTCAGCTGGAAATT+GGG - chr4.2:25893402-25893421 None:intergenic 45.0%
TGAAACGAATTGTCGGAACG+TGG - chr4.2:25893366-25893385 None:intergenic 45.0%
TGATATCTTCCTGACTCAGC+TGG - chr4.2:25893410-25893429 None:intergenic 45.0%
TTCACTGTACTGCTAATGCC+AGG - chr4.2:25895266-25895285 None:intergenic 45.0%
TTCCTGACTCAGCTGGAAAT+TGG - chr4.2:25893403-25893422 None:intergenic 45.0%
! CATTTGGGATCGTTCCTATG+GGG + chr4.2:25895336-25895355 MS.gene006897:CDS 45.0%
! CCATTTGGGATCGTTCCTAT+GGG + chr4.2:25895335-25895354 MS.gene006897:CDS 45.0%
! CCTGAATGTCTTCGCCTTTT+AGG - chr4.2:25894667-25894686 None:intergenic 45.0%
! GAGGTGGGTCTTATGTCTTT+GGG + chr4.2:25894445-25894464 MS.gene006897:intron 45.0%
! TCCATTTGGGATCGTTCCTA+TGG + chr4.2:25895334-25895353 MS.gene006897:CDS 45.0%
! TGATGCTTTGGACAAATGCG+AGG + chr4.2:25894231-25894250 MS.gene006897:CDS 45.0%
!! ACCTTTAAGACCTAACGCTC+AGG - chr4.2:25894579-25894598 None:intergenic 45.0%
!! CCTTTAAGACCTAACGCTCA+GGG - chr4.2:25894578-25894597 None:intergenic 45.0%
AAATCAACCTCAGCGGAGGT+GGG + chr4.2:25894430-25894449 MS.gene006897:intron 50.0%
AAGGATCAGGTTCAGCTCCA+AGG - chr4.2:25893698-25893717 None:intergenic 50.0%
AGGACCTCGGTATTCGATTC+AGG - chr4.2:25893678-25893697 None:intergenic 50.0%
CCCATAGGAACGATCCCAAA+TGG - chr4.2:25895338-25895357 None:intergenic 50.0%
CCCTGAGCGTTAGGTCTTAA+AGG + chr4.2:25894575-25894594 MS.gene006897:intron 50.0%
GAATCGAATACCGAGGTCCT+TGG + chr4.2:25893678-25893697 MS.gene006897:CDS 50.0%
TAAATCAACCTCAGCGGAGG+TGG + chr4.2:25894429-25894448 MS.gene006897:intron 50.0%
TCCCAATTTCCAGCTGAGTC+AGG + chr4.2:25893398-25893417 MS.gene006897:CDS 50.0%
TGCGACTTTGTGTGTGGGTT+GGG + chr4.2:25894351-25894370 MS.gene006897:intron 50.0%
TGTTTGGCAACTCAGTCCAC+TGG - chr4.2:25894611-25894630 None:intergenic 50.0%
TTCTCTTGCAGCCTGCACAA+AGG - chr4.2:25894206-25894225 None:intergenic 50.0%
TTGCTCCTCACGACAGAGAA+AGG + chr4.2:25895375-25895394 MS.gene006897:CDS 50.0%
! GGAGGTGGGTCTTATGTCTT+TGG + chr4.2:25894444-25894463 MS.gene006897:intron 50.0%
! TAGAAGACCGACTCCATCAG+AGG + chr4.2:25894486-25894505 MS.gene006897:intron 50.0%
!! TAAAGGTGAAGGCGTTCCAG+TGG + chr4.2:25894592-25894611 MS.gene006897:intron 50.0%
AGAACACTGCCCTGAGCGTT+AGG + chr4.2:25894566-25894585 MS.gene006897:intron 55.0%
AGGTTCAGCTCCAAGGACCT+CGG - chr4.2:25893691-25893710 None:intergenic 55.0%
GTGCGACTTTGTGTGTGGGT+TGG + chr4.2:25894350-25894369 MS.gene006897:intron 55.0%
! CCTTTTAGGCTTGACGCTCG+GGG - chr4.2:25894653-25894672 None:intergenic 55.0%
! CGCCTTTTAGGCTTGACGCT+CGG - chr4.2:25894655-25894674 None:intergenic 55.0%
! CTTTTAGGCTTGACGCTCGG+GGG - chr4.2:25894652-25894671 None:intergenic 55.0%
! GCCTTTTAGGCTTGACGCTC+GGG - chr4.2:25894654-25894673 None:intergenic 55.0%
CATAAGACCCACCTCCGCTG+AGG - chr4.2:25894440-25894459 None:intergenic 60.0%
CCCCGAGCGTCAAGCCTAAA+AGG + chr4.2:25894650-25894669 MS.gene006897:intron 60.0%
! GCAAGGCATCTGCAAGCCCA+AGG - chr4.2:25893455-25893474 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 25893305 25895409 25893305 ID=MS.gene006897
chr4.2 mRNA 25893305 25895409 25893305 ID=MS.gene006897.t1;Parent=MS.gene006897
chr4.2 exon 25893305 25893517 25893305 ID=MS.gene006897.t1.exon1;Parent=MS.gene006897.t1
chr4.2 CDS 25893305 25893517 25893305 ID=cds.MS.gene006897.t1;Parent=MS.gene006897.t1
chr4.2 exon 25893615 25893791 25893615 ID=MS.gene006897.t1.exon2;Parent=MS.gene006897.t1
chr4.2 CDS 25893615 25893791 25893615 ID=cds.MS.gene006897.t1;Parent=MS.gene006897.t1
chr4.2 exon 25893894 25894118 25893894 ID=MS.gene006897.t1.exon3;Parent=MS.gene006897.t1
chr4.2 CDS 25893894 25894118 25893894 ID=cds.MS.gene006897.t1;Parent=MS.gene006897.t1
chr4.2 exon 25894214 25894342 25894214 ID=MS.gene006897.t1.exon4;Parent=MS.gene006897.t1
chr4.2 CDS 25894214 25894342 25894214 ID=cds.MS.gene006897.t1;Parent=MS.gene006897.t1
chr4.2 exon 25895176 25895409 25895176 ID=MS.gene006897.t1.exon5;Parent=MS.gene006897.t1
chr4.2 CDS 25895176 25895409 25895176 ID=cds.MS.gene006897.t1;Parent=MS.gene006897.t1
Gene Sequence

>MS.gene006897

ATGGTGCGATCATCATTGGATGAGATATCAGACTCGGGAGCATTTACGAGATCAGCTTCCACGTTCCGACAATTCGTTTCAAAGGATCCAAATTCCCAATTTCCAGCTGAGTCAGGAAGATATCATCTCTACATATCATATGCTTGTCCTTGGGCTTGCAGATGCCTTGCTTACTTGAAAATCAAAGGACTTGACAAAGCCATCAGTTTCTCGGTTAGTAGGTTTTTTTGCTTAATATTTGCAAGTGGTGTGATTTCAGTTTTCAATAGTTCTTACTTGGAATTTCATATCTTGCATGTTTAATAATCAGTCGGTCAAACCTATATGGGGAAGAACTAAAGAATCAGATGAATATATGGGATGGATTTTTCCTGAATCGAATACCGAGGTCCTTGGAGCTGAACCTGATCCTTTGAATGGAGCAAAGAGTGTTAGAGAACTTTATGAAATTGCGAGTACAAACTACTCCGGAAAGTTCACTGTTCCTGTAGGTTTTTGCTTTAGAATGGACTTGTTTCTTTCTTAGCTTTTGTGATTTGCTTTTCTCAGTAGATTTCTATTGTCAGCTGATTGATTTTGATCTGCACAGATTCTGTGGGATAAGAAACTCAAGACAATTGTTAACAACGAGAGCTCGGAGATAATACGCATGTTTAATACTGAATTCAACGATATTGCAGAAAATCCCATCTTGGACTTGTATCCCTCTGAATTGCAAGCCCAAATTGATGAGACTAATGAGTGGATATATCCTAATATAAACAACGGTGTTTATAGATGTGGGTTTGCAAAGAAGCAGGAACCATACATTGATGTATGTATCTATTTTTTACTTTTTCAGGTGTAGTTAATAATTTAATGTTTACATTCATTTAGCATTTGAAACTGTTCATTTGGTCCTTTGTGCAGGCTGCAAGAGAATTATATGATGCTTTGGACAAATGCGAGGATATACTAAGCAAGCAACGCTATATATGTGGCAACAAGCTTACTGAAGCAGACATTCGTTTGTTTGTCACTCTCATTAGATTTGATGAGGTATAGTGTGCGACTTTGTGTGTGGGTTGGGCACCTATTCTTGATATTTGGGAGTATCTTATCATCATTGTCCTTGTCTGAATTAATAAATCAACCTCAGCGGAGGTGGGTCTTATGTCTTTGGGCCATTCATAGAATGAGATTAGAAGACCGACTCCATCAGAGGATTGAGTTGAACGAGTACCCTAGTAACCTTTGCTGGTATACTTGGTCATATTAGTAAAGAACACTGCCCTGAGCGTTAGGTCTTAAAGGTGAAGGCGTTCCAGTGGACTGAGTTGCCAAACAGTTTGGTACATAACATAGCCCCCGAGCGTCAAGCCTAAAAGGCGAAGACATTCAGGTGAACCGAGTGCTCAAACTGTTTGATACCGAATTTCAAAAACCAAATTGCACCCCTAGTTTTAAGACTGCGTAACTTGTCAAAAATATGGAGTCTGTGTTGTCAAATTTCATTCTTTTAAAAACATTAAAATTCAATATGGCCCTCTCCAAACCTCTCTATCTAAACATATCCTTAGGCACCACATGCATTAATCAGTAAACTCACCAAGTGTTTCCTTCTTGTTATTGCTTTATTATTATTCGATGGACACTTAATGGTTTAACTTATAGCATGCTAAAGAGGAAAGCTTTGAAGTGGAACTCAAATTCTGAAGTTTTTTCTTCATTCTTTTTGCGCTTTTAATATATATCTGTTCAACATTTTGATGGAATTAGAGGAACATACAAGAACTGGGGTGATTGCTTTCCTATTTTGTGACTCATTTTGTTGCAATCTACCCATCCCGATGTTTTGATTTTTAAGTAATTCGCCATTGTTATAATTGCAGGTTTATGCAGTTCACTTTAAGTGTAACAAGAAGCTAATACGGGAATATCCAAATATCTTCAATTACACCAAAGACATTTTCCAAATCCCTGGCATTAGCAGTACAGTGAACATGGAACATATAAAGTTGCATTACTATGGAAGTCATCCTTCTATCAATCCATTTGGGATCGTTCCTATGGGGCCAAATATCGATTATTTTGCTCCTCACGACAGAGAAAGGTTTTCTACTTAG
Protein sequence

>MS.gene006897.t1

MVRSSLDEISDSGAFTRSASTFRQFVSKDPNSQFPAESGRYHLYISYACPWACRCLAYLKIKGLDKAISFSSVKPIWGRTKESDEYMGWIFPESNTEVLGAEPDPLNGAKSVRELYEIASTNYSGKFTVPILWDKKLKTIVNNESSEIIRMFNTEFNDIAENPILDLYPSELQAQIDETNEWIYPNINNGVYRCGFAKKQEPYIDAARELYDALDKCEDILSKQRYICGNKLTEADIRLFVTLIRFDEVYAVHFKCNKKLIREYPNIFNYTKDIFQIPGISSTVNMEHIKLHYYGSHPSINPFGIVPMGPNIDYFAPHDRERFST