Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038386.t1 | XP_003617481.1 | 98.2 | 282 | 1 | 1 | 1 | 282 | 12 | 289 | 1.70E-147 | 531.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038386.t1 | P87027 | 47.1 | 187 | 99 | 0 | 96 | 282 | 9 | 195 | 6.4e-50 | 199.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038386.t1 | G7K3G2 | 98.2 | 282 | 1 | 1 | 1 | 282 | 12 | 289 | 1.3e-147 | 531.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene95545 | MS.gene038386 | PPI |
| MS.gene027416 | MS.gene038386 | PPI |
| MS.gene038386 | MS.gene87637 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038386.t1 | MTR_5g092060 | 97.518 | 282 | 3 | 1 | 1 | 282 | 12 | 289 | 0.0 | 568 |
| MS.gene038386.t1 | MTR_4g047870 | 63.118 | 263 | 90 | 2 | 20 | 282 | 21 | 276 | 2.99e-112 | 325 |
| MS.gene038386.t1 | MTR_5g013900 | 27.485 | 171 | 118 | 3 | 93 | 262 | 5 | 170 | 1.12e-16 | 77.0 |
| MS.gene038386.t1 | MTR_1g062760 | 27.072 | 181 | 121 | 5 | 96 | 272 | 13 | 186 | 5.61e-16 | 75.1 |
| MS.gene038386.t1 | MTR_4g098950 | 26.316 | 171 | 120 | 3 | 93 | 262 | 5 | 170 | 7.29e-16 | 74.7 |
| MS.gene038386.t1 | MTR_4g064897 | 28.824 | 170 | 113 | 2 | 98 | 265 | 13 | 176 | 8.14e-16 | 74.7 |
| MS.gene038386.t1 | MTR_3g064390 | 30.588 | 170 | 108 | 4 | 96 | 262 | 9 | 171 | 1.17e-15 | 73.9 |
| MS.gene038386.t1 | MTR_3g069510 | 29.412 | 170 | 112 | 2 | 98 | 265 | 13 | 176 | 3.11e-15 | 73.2 |
| MS.gene038386.t1 | MTR_2g005510 | 27.811 | 169 | 116 | 2 | 98 | 265 | 16 | 179 | 3.79e-15 | 73.2 |
| MS.gene038386.t1 | MTR_5g076590 | 30.588 | 170 | 108 | 4 | 96 | 262 | 9 | 171 | 9.38e-15 | 71.6 |
| MS.gene038386.t1 | MTR_2g075950 | 28.415 | 183 | 118 | 5 | 88 | 265 | 3 | 177 | 1.85e-14 | 71.2 |
| MS.gene038386.t1 | MTR_2g083860 | 27.717 | 184 | 118 | 6 | 88 | 265 | 3 | 177 | 4.65e-14 | 70.1 |
| MS.gene038386.t1 | MTR_4g079350 | 25.521 | 192 | 134 | 4 | 88 | 277 | 3 | 187 | 7.82e-14 | 69.3 |
| MS.gene038386.t1 | MTR_1g078200 | 24.862 | 181 | 129 | 3 | 86 | 265 | 4 | 178 | 1.40e-13 | 68.9 |
| MS.gene038386.t1 | MTR_3g086980 | 26.744 | 172 | 114 | 5 | 98 | 265 | 17 | 180 | 1.54e-13 | 68.6 |
| MS.gene038386.t1 | MTR_3g087160 | 25.146 | 171 | 122 | 3 | 93 | 262 | 5 | 170 | 1.63e-13 | 68.2 |
| MS.gene038386.t1 | MTR_0262s0050 | 27.072 | 181 | 119 | 6 | 89 | 265 | 8 | 179 | 1.97e-13 | 68.6 |
| MS.gene038386.t1 | MTR_1g068730 | 26.190 | 168 | 114 | 4 | 91 | 255 | 10 | 170 | 2.47e-13 | 68.2 |
| MS.gene038386.t1 | MTR_8g090215 | 24.561 | 171 | 123 | 3 | 93 | 262 | 5 | 170 | 2.54e-13 | 67.8 |
| MS.gene038386.t1 | MTR_7g103070 | 26.923 | 182 | 121 | 6 | 96 | 272 | 15 | 189 | 3.63e-13 | 67.4 |
| MS.gene038386.t1 | MTR_1g068740 | 26.190 | 168 | 114 | 4 | 91 | 255 | 10 | 170 | 4.26e-13 | 67.4 |
| MS.gene038386.t1 | MTR_8g103550 | 26.667 | 165 | 115 | 2 | 99 | 262 | 11 | 170 | 5.09e-13 | 66.6 |
| MS.gene038386.t1 | MTR_7g051940 | 24.118 | 170 | 119 | 4 | 98 | 264 | 14 | 176 | 5.27e-13 | 67.0 |
| MS.gene038386.t1 | MTR_1g052475 | 28.070 | 171 | 113 | 4 | 98 | 265 | 16 | 179 | 5.47e-13 | 67.0 |
| MS.gene038386.t1 | MTR_7g100020 | 25.466 | 161 | 111 | 3 | 98 | 255 | 16 | 170 | 5.67e-13 | 67.0 |
| MS.gene038386.t1 | MTR_8g089985 | 26.744 | 172 | 114 | 5 | 98 | 265 | 18 | 181 | 8.42e-13 | 66.6 |
| MS.gene038386.t1 | MTR_2g096550 | 23.125 | 160 | 117 | 2 | 98 | 255 | 16 | 171 | 1.16e-12 | 66.2 |
| MS.gene038386.t1 | MTR_7g405790 | 23.214 | 168 | 123 | 2 | 98 | 264 | 16 | 178 | 1.36e-12 | 65.9 |
| MS.gene038386.t1 | MTR_1g115325 | 24.845 | 161 | 112 | 3 | 98 | 255 | 16 | 170 | 1.70e-12 | 65.5 |
| MS.gene038386.t1 | MTR_2g005500 | 23.810 | 168 | 122 | 2 | 98 | 264 | 10 | 172 | 1.79e-12 | 65.5 |
| MS.gene038386.t1 | MTR_4g088090 | 23.958 | 192 | 137 | 4 | 88 | 277 | 3 | 187 | 2.30e-12 | 65.5 |
| MS.gene038386.t1 | MTR_1g090717 | 25.556 | 180 | 125 | 4 | 97 | 272 | 15 | 189 | 4.01e-12 | 64.7 |
| MS.gene038386.t1 | MTR_2g014990 | 24.479 | 192 | 136 | 4 | 88 | 277 | 3 | 187 | 4.94e-12 | 64.3 |
| MS.gene038386.t1 | MTR_3g064390 | 30.508 | 118 | 81 | 1 | 96 | 212 | 9 | 126 | 7.30e-12 | 62.8 |
| MS.gene038386.t1 | MTR_2g090865 | 24.852 | 169 | 121 | 2 | 98 | 265 | 12 | 175 | 1.22e-11 | 63.2 |
| MS.gene038386.t1 | MTR_3g107717 | 26.012 | 173 | 121 | 1 | 94 | 266 | 10 | 175 | 1.57e-11 | 63.2 |
| MS.gene038386.t1 | MTR_3g107713 | 26.012 | 173 | 121 | 1 | 94 | 266 | 10 | 175 | 1.62e-11 | 62.8 |
| MS.gene038386.t1 | MTR_3g107707 | 26.012 | 173 | 121 | 1 | 94 | 266 | 10 | 175 | 1.68e-11 | 62.8 |
| MS.gene038386.t1 | MTR_7g117910 | 29.771 | 131 | 87 | 2 | 87 | 212 | 6 | 136 | 2.24e-11 | 62.4 |
| MS.gene038386.t1 | MTR_8g027220 | 26.316 | 190 | 125 | 5 | 81 | 262 | 5 | 187 | 5.09e-11 | 61.6 |
| MS.gene038386.t1 | MTR_4g063160 | 25.153 | 163 | 111 | 4 | 98 | 255 | 16 | 172 | 5.47e-11 | 61.2 |
| MS.gene038386.t1 | MTR_8g036220 | 25.294 | 170 | 115 | 5 | 98 | 263 | 17 | 178 | 9.56e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038386.t1 | AT5G54840 | 63.636 | 286 | 89 | 5 | 1 | 282 | 12 | 286 | 1.14e-118 | 342 |
| MS.gene038386.t1 | AT3G21700 | 53.535 | 297 | 118 | 6 | 1 | 282 | 1 | 292 | 4.53e-104 | 305 |
| MS.gene038386.t1 | AT3G21700 | 53.199 | 297 | 118 | 7 | 1 | 282 | 1 | 291 | 7.56e-102 | 299 |
| MS.gene038386.t1 | AT3G21700 | 47.737 | 243 | 106 | 7 | 1 | 228 | 1 | 237 | 5.15e-66 | 206 |
| MS.gene038386.t1 | AT5G54840 | 54.902 | 204 | 77 | 5 | 1 | 200 | 12 | 204 | 1.15e-60 | 192 |
| MS.gene038386.t1 | AT5G54840 | 54.902 | 204 | 77 | 5 | 1 | 200 | 12 | 204 | 3.54e-60 | 191 |
| MS.gene038386.t1 | AT2G44610 | 26.316 | 171 | 120 | 2 | 93 | 262 | 5 | 170 | 3.82e-17 | 78.6 |
| MS.gene038386.t1 | AT5G59150 | 27.841 | 176 | 122 | 2 | 90 | 265 | 6 | 176 | 2.07e-16 | 76.6 |
| MS.gene038386.t1 | AT1G09630 | 27.604 | 192 | 124 | 3 | 98 | 279 | 13 | 199 | 3.95e-16 | 75.9 |
| MS.gene038386.t1 | AT1G07410 | 28.235 | 170 | 114 | 3 | 98 | 265 | 13 | 176 | 9.55e-16 | 74.7 |
| MS.gene038386.t1 | AT3G46830 | 28.824 | 170 | 113 | 2 | 98 | 265 | 13 | 176 | 1.04e-15 | 74.7 |
| MS.gene038386.t1 | AT3G46830 | 28.824 | 170 | 113 | 2 | 98 | 265 | 13 | 176 | 1.04e-15 | 74.7 |
| MS.gene038386.t1 | AT1G05810 | 26.471 | 170 | 115 | 4 | 98 | 264 | 13 | 175 | 3.72e-15 | 73.2 |
| MS.gene038386.t1 | AT1G05810 | 26.471 | 170 | 115 | 4 | 98 | 264 | 59 | 221 | 8.24e-15 | 73.2 |
| MS.gene038386.t1 | AT5G45130 | 29.412 | 170 | 110 | 4 | 96 | 262 | 9 | 171 | 8.80e-15 | 71.6 |
| MS.gene038386.t1 | AT4G19640 | 29.412 | 170 | 110 | 4 | 96 | 262 | 9 | 171 | 1.10e-14 | 71.6 |
| MS.gene038386.t1 | AT1G16920 | 26.289 | 194 | 130 | 5 | 88 | 277 | 3 | 187 | 1.36e-14 | 71.6 |
| MS.gene038386.t1 | AT5G03530 | 26.111 | 180 | 124 | 4 | 96 | 272 | 12 | 185 | 1.55e-14 | 71.2 |
| MS.gene038386.t1 | AT5G03530 | 26.111 | 180 | 124 | 4 | 96 | 272 | 12 | 185 | 1.55e-14 | 71.2 |
| MS.gene038386.t1 | AT4G18800 | 28.415 | 183 | 118 | 5 | 88 | 265 | 3 | 177 | 2.19e-14 | 70.9 |
| MS.gene038386.t1 | AT5G65270 | 27.128 | 188 | 121 | 6 | 85 | 265 | 3 | 181 | 2.30e-14 | 71.2 |
| MS.gene038386.t1 | AT3G07410 | 27.647 | 170 | 113 | 4 | 98 | 264 | 13 | 175 | 3.35e-14 | 70.5 |
| MS.gene038386.t1 | AT4G39890 | 25.000 | 172 | 122 | 4 | 93 | 262 | 5 | 171 | 3.71e-14 | 70.5 |
| MS.gene038386.t1 | AT2G43130 | 26.471 | 170 | 115 | 4 | 98 | 264 | 13 | 175 | 3.93e-14 | 70.1 |
| MS.gene038386.t1 | AT2G31680 | 26.036 | 169 | 115 | 4 | 98 | 263 | 13 | 174 | 4.43e-14 | 70.1 |
| MS.gene038386.t1 | AT4G39990 | 27.647 | 170 | 113 | 4 | 98 | 264 | 18 | 180 | 4.98e-14 | 70.1 |
| MS.gene038386.t1 | AT5G55080 | 28.090 | 178 | 111 | 4 | 90 | 262 | 6 | 171 | 5.23e-14 | 70.1 |
| MS.gene038386.t1 | AT5G45750 | 28.415 | 183 | 118 | 5 | 88 | 265 | 3 | 177 | 7.37e-14 | 69.7 |
| MS.gene038386.t1 | AT1G06400 | 28.022 | 182 | 120 | 5 | 88 | 265 | 3 | 177 | 1.53e-13 | 68.6 |
| MS.gene038386.t1 | AT3G15060 | 25.967 | 181 | 127 | 3 | 88 | 266 | 3 | 178 | 1.54e-13 | 68.6 |
| MS.gene038386.t1 | AT3G09900 | 26.190 | 168 | 114 | 4 | 91 | 255 | 10 | 170 | 1.84e-13 | 68.6 |
| MS.gene038386.t1 | AT5G10260 | 23.977 | 171 | 124 | 2 | 93 | 262 | 5 | 170 | 1.88e-13 | 68.2 |
| MS.gene038386.t1 | AT5G47520 | 24.852 | 169 | 121 | 2 | 98 | 265 | 15 | 178 | 1.92e-13 | 68.6 |
| MS.gene038386.t1 | AT3G53610 | 26.347 | 167 | 113 | 4 | 98 | 261 | 16 | 175 | 1.93e-13 | 68.2 |
| MS.gene038386.t1 | AT3G53610 | 26.347 | 167 | 113 | 4 | 98 | 261 | 16 | 175 | 1.93e-13 | 68.2 |
| MS.gene038386.t1 | AT3G53610 | 26.347 | 167 | 113 | 4 | 98 | 261 | 16 | 175 | 1.93e-13 | 68.2 |
| MS.gene038386.t1 | AT5G03520 | 26.190 | 168 | 114 | 4 | 91 | 255 | 10 | 170 | 3.35e-13 | 67.8 |
| MS.gene038386.t1 | AT2G22290 | 25.434 | 173 | 119 | 4 | 93 | 262 | 5 | 170 | 3.80e-13 | 67.4 |
| MS.gene038386.t1 | AT5G59840 | 26.347 | 167 | 113 | 4 | 98 | 261 | 16 | 175 | 6.49e-13 | 67.0 |
| MS.gene038386.t1 | AT5G45130 | 29.268 | 164 | 106 | 4 | 102 | 262 | 23 | 179 | 7.65e-13 | 66.6 |
| MS.gene038386.t1 | AT5G60860 | 25.000 | 180 | 128 | 3 | 88 | 265 | 3 | 177 | 9.67e-13 | 66.2 |
| MS.gene038386.t1 | AT3G46060 | 25.466 | 161 | 111 | 3 | 98 | 255 | 16 | 170 | 1.17e-12 | 66.2 |
| MS.gene038386.t1 | AT3G46060 | 25.466 | 161 | 111 | 3 | 98 | 255 | 16 | 170 | 1.17e-12 | 66.2 |
| MS.gene038386.t1 | AT3G46060 | 25.466 | 161 | 111 | 3 | 98 | 255 | 16 | 170 | 1.17e-12 | 66.2 |
| MS.gene038386.t1 | AT3G12160 | 26.316 | 171 | 116 | 4 | 98 | 265 | 16 | 179 | 1.18e-12 | 66.2 |
| MS.gene038386.t1 | AT4G18430 | 26.087 | 184 | 123 | 6 | 87 | 265 | 2 | 177 | 1.49e-12 | 65.9 |
| MS.gene038386.t1 | AT4G19640 | 29.661 | 118 | 82 | 1 | 96 | 212 | 9 | 126 | 1.83e-12 | 64.3 |
| MS.gene038386.t1 | AT4G19640 | 29.661 | 118 | 82 | 1 | 96 | 212 | 9 | 126 | 2.35e-12 | 64.7 |
| MS.gene038386.t1 | AT1G01200 | 25.882 | 170 | 117 | 4 | 98 | 264 | 29 | 192 | 5.76e-12 | 64.7 |
| MS.gene038386.t1 | AT5G47960 | 26.163 | 172 | 115 | 5 | 98 | 265 | 16 | 179 | 1.25e-11 | 63.5 |
| MS.gene038386.t1 | AT3G11730 | 25.904 | 166 | 117 | 3 | 98 | 262 | 9 | 169 | 1.34e-11 | 62.8 |
| MS.gene038386.t1 | AT3G54840 | 25.137 | 183 | 126 | 4 | 84 | 262 | 20 | 195 | 2.02e-11 | 62.4 |
| MS.gene038386.t1 | AT5G20020 | 26.036 | 169 | 118 | 1 | 94 | 262 | 10 | 171 | 3.66e-11 | 62.0 |
| MS.gene038386.t1 | AT5G20010 | 26.036 | 169 | 118 | 1 | 94 | 262 | 10 | 171 | 3.69e-11 | 62.0 |
| MS.gene038386.t1 | AT5G55190 | 26.036 | 169 | 118 | 1 | 94 | 262 | 10 | 171 | 3.99e-11 | 62.0 |
Find 54 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATTTAGAAGTCAATTA+TGG | 0.162376 | 5.4:+73031713 | None:intergenic |
| GTTCAGGTTGATGTTAAATA+TGG | 0.230732 | 5.4:-73034088 | MS.gene038386:CDS |
| AATGGAAGGATTGAATTTAA+TGG | 0.251785 | 5.4:-73033457 | MS.gene038386:CDS |
| ACAATTGTCCATTGTAAATC+TGG | 0.259047 | 5.4:+73032117 | None:intergenic |
| GAACAACAGCATACCAATAA+TGG | 0.260980 | 5.4:-73033872 | MS.gene038386:CDS |
| ATCTGACTCATACCCATTAT+TGG | 0.276525 | 5.4:+73033859 | None:intergenic |
| GTTCATTTGGAATAAACTTA+TGG | 0.296115 | 5.4:-73034026 | MS.gene038386:CDS |
| ACAATTTAGAAGTCAATTAT+GGG | 0.359970 | 5.4:+73031714 | None:intergenic |
| ACTGCAATTCCCATTCTAAT+AGG | 0.364632 | 5.4:-73032171 | MS.gene038386:CDS |
| TTGTTGGGTGATTGCCATAT+TGG | 0.368640 | 5.4:-73033809 | MS.gene038386:CDS |
| CAAAGCTCTTTAACTTGCCT+TGG | 0.379264 | 5.4:-73031767 | MS.gene038386:CDS |
| AAACTTATGGTTTGTTCTGT+TGG | 0.381679 | 5.4:-73034013 | MS.gene038386:CDS |
| ATAGAAAGAAATTTGAAAGT+TGG | 0.387772 | 5.4:-73031741 | MS.gene038386:CDS |
| AAACTTGGTTCCTATTAGAA+TGG | 0.395511 | 5.4:+73032161 | None:intergenic |
| AACCTTAAGATTAGTTTGTT+GGG | 0.398052 | 5.4:-73033824 | MS.gene038386:CDS |
| TGTTGGGTGATTGCCATATT+GGG | 0.408372 | 5.4:-73033808 | MS.gene038386:CDS |
| GACAAAACTTTGTATGTTCA+AGG | 0.421423 | 5.4:-73033435 | MS.gene038386:CDS |
| ACTCTGCAGGAAAATTGTTC+AGG | 0.422078 | 5.4:-73034104 | MS.gene038386:CDS |
| GTGTTGCACTTGAAAAGAAA+AGG | 0.422846 | 5.4:+73031829 | None:intergenic |
| CACCCAACAAACTAATCTTA+AGG | 0.431894 | 5.4:+73033822 | None:intergenic |
| TTAAATATGGTATGCTTCAA+AGG | 0.431941 | 5.4:-73034075 | MS.gene038386:CDS |
| CAATTGTCCATTGTAAATCT+GGG | 0.433079 | 5.4:+73032118 | None:intergenic |
| AACAACAGCATACCAATAAT+GGG | 0.438949 | 5.4:-73033871 | MS.gene038386:CDS |
| TCTCCGGCTACGGTTGATGA+TGG | 0.441368 | 5.4:-73033905 | MS.gene038386:CDS |
| AACTGCATCTTTACAAGCCA+TGG | 0.445088 | 5.4:+73033179 | None:intergenic |
| GGCTTATGCAAGGGCAATGA+AGG | 0.452873 | 5.4:-73031856 | MS.gene038386:CDS |
| GAACCTTAAGATTAGTTTGT+TGG | 0.454454 | 5.4:-73033825 | MS.gene038386:CDS |
| AGAAAAGAGGAGTTTGCAAA+TGG | 0.455607 | 5.4:-73033475 | MS.gene038386:CDS |
| AACTTGGTTCCTATTAGAAT+GGG | 0.455621 | 5.4:+73032162 | None:intergenic |
| GCCGTTGTCGCCGCCGTCTC+CGG | 0.465432 | 5.4:-73033921 | MS.gene038386:CDS |
| GTCGCCGCCGTCTCCGGCTA+CGG | 0.469833 | 5.4:-73033915 | MS.gene038386:CDS |
| TGAAGCAAGAAAGTGGAATC+AGG | 0.481024 | 5.4:-73032567 | MS.gene038386:intron |
| AAGAGGAGTTTGCAAATGGA+AGG | 0.488557 | 5.4:-73033471 | MS.gene038386:CDS |
| GTATGAGTCAGATTCTGATT+TGG | 0.522092 | 5.4:-73033849 | MS.gene038386:CDS |
| TAAATATGGTATGCTTCAAA+GGG | 0.523765 | 5.4:-73034074 | MS.gene038386:CDS |
| AAATTTCTTTCTATCGTCCA+AGG | 0.528916 | 5.4:+73031750 | None:intergenic |
| AAACCATCATCAACCGTAGC+CGG | 0.529864 | 5.4:+73033902 | None:intergenic |
| TCTAACAAAATCATCAAACT+TGG | 0.542555 | 5.4:+73032146 | None:intergenic |
| AGAAGATGACACAACTCTGC+AGG | 0.552513 | 5.4:-73034117 | None:intergenic |
| GCCGGAGACGGCGGCGACAA+CGG | 0.555503 | 5.4:+73033920 | None:intergenic |
| TACAGGCAAGGGCTTATGCA+AGG | 0.559748 | 5.4:-73031866 | MS.gene038386:intron |
| TGTTGCACTTGAAAAGAAAA+GGG | 0.560066 | 5.4:+73031830 | None:intergenic |
| TAGGAAATGAGGTAGAAAAG+AGG | 0.564038 | 5.4:-73033488 | MS.gene038386:CDS |
| GTCCTTGGATCAAATTCCCA+TGG | 0.573998 | 5.4:-73033196 | MS.gene038386:CDS |
| TCATCAACCGTAGCCGGAGA+CGG | 0.574660 | 5.4:+73033908 | None:intergenic |
| GGTACAGTGAAGCAAGAAAG+TGG | 0.579957 | 5.4:-73032574 | MS.gene038386:CDS |
| AATTGTCCATTGTAAATCTG+GGG | 0.582951 | 5.4:+73032119 | None:intergenic |
| GATCAAGTACGTAGGAAATG+AGG | 0.589406 | 5.4:-73033499 | MS.gene038386:CDS |
| ACAATGGACAATTGTAACAC+AGG | 0.603332 | 5.4:-73032109 | MS.gene038386:intron |
| ACAGGCAAGGGCTTATGCAA+GGG | 0.634009 | 5.4:-73031865 | MS.gene038386:intron |
| ACTGCATCTTTACAAGCCAT+GGG | 0.635433 | 5.4:+73033180 | None:intergenic |
| AGCCATGGGAATTTGATCCA+AGG | 0.654764 | 5.4:+73033194 | None:intergenic |
| ATTGTCCATTGTAAATCTGG+GGG | 0.655553 | 5.4:+73032120 | None:intergenic |
| TCAACCGTAGCCGGAGACGG+CGG | 0.662086 | 5.4:+73033911 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAAATATAGTAATATTT+CGG | + | chr5.4:73033148-73033167 | None:intergenic | 10.0% |
| !! | AAAACATAATACATCTAATT+AGG | + | chr5.4:73033602-73033621 | None:intergenic | 15.0% |
| !! | AAACATAATACATCTAATTA+GGG | + | chr5.4:73033601-73033620 | None:intergenic | 15.0% |
| !! | AACTAAAAATGTTAAGTTAA+AGG | + | chr5.4:73032827-73032846 | None:intergenic | 15.0% |
| !! | AATAAAAAAGCCAAATAATA+TGG | + | chr5.4:73033914-73033933 | None:intergenic | 15.0% |
| !!! | AAAAGTAACACTTTATTTAA+AGG | + | chr5.4:73033889-73033908 | None:intergenic | 15.0% |
| !!! | ATTAGATGTATTATGTTTTA+TGG | - | chr5.4:73033603-73033622 | MS.gene038386:intron | 15.0% |
| !!! | TTACTTTTTACCATATTATT+TGG | - | chr5.4:73033901-73033920 | MS.gene038386:CDS | 15.0% |
| !!! | TTTTTTAAGTTAGTTATTGA+TGG | - | chr5.4:73032576-73032595 | MS.gene038386:CDS | 15.0% |
| !! | AACCTCAAAATTTAGTTAAA+AGG | - | chr5.4:73033105-73033124 | MS.gene038386:intron | 20.0% |
| !! | ATAGAAAGAAATTTGAAAGT+TGG | - | chr5.4:73034090-73034109 | MS.gene038386:CDS | 20.0% |
| !! | ATTGATGGAAAATTATTATG+TGG | - | chr5.4:73032591-73032610 | MS.gene038386:intron | 20.0% |
| !! | TAAAAAATAAATGCAGAACA+TGG | + | chr5.4:73032503-73032522 | None:intergenic | 20.0% |
| !! | TAACCTCAAAATTAAGTTAA+GGG | + | chr5.4:73032768-73032787 | None:intergenic | 20.0% |
| !! | TCGAAAATCATATAATTCAA+AGG | + | chr5.4:73032224-73032243 | None:intergenic | 20.0% |
| !! | TGTATAAGTTACGAATATAA+TGG | - | chr5.4:73033179-73033198 | MS.gene038386:CDS | 20.0% |
| !! | TTAACCTCAAAATTAAGTTA+AGG | + | chr5.4:73032769-73032788 | None:intergenic | 20.0% |
| !! | TTCTTACTATAATAGTTCAA+AGG | + | chr5.4:73033401-73033420 | None:intergenic | 20.0% |
| !! | TTGATGGAAAATTATTATGT+GGG | - | chr5.4:73032592-73032611 | MS.gene038386:intron | 20.0% |
| !! | TTTACCTTAAATGTTGAAAT+GGG | - | chr5.4:73032078-73032097 | MS.gene038386:intron | 20.0% |
| !!! | CAAAATTTTCAAGTTTATCA+TGG | - | chr5.4:73034041-73034060 | MS.gene038386:CDS | 20.0% |
| !!! | GTGACACTTTTTAAAATTAT+AGG | + | chr5.4:73032798-73032817 | None:intergenic | 20.0% |
| !!! | TAACATTTTTAGTTCATATG+TGG | - | chr5.4:73032833-73032852 | MS.gene038386:intron | 20.0% |
| !!! | TATAGTAAGAAAAAGTTTTG+AGG | - | chr5.4:73033410-73033429 | MS.gene038386:CDS | 20.0% |
| !!! | TCGTATTTCATTTTGTATAT+GGG | - | chr5.4:73032422-73032441 | MS.gene038386:intron | 20.0% |
| !!! | TTATATGATTTTCGATTTGT+TGG | - | chr5.4:73032229-73032248 | MS.gene038386:intron | 20.0% |
| !!! | TTTTTTAGATTTCTCTCATT+AGG | - | chr5.4:73032516-73032535 | MS.gene038386:intron | 20.0% |
| ! | AATGGAAGGATTGAATTTAA+TGG | - | chr5.4:73032374-73032393 | MS.gene038386:intron | 25.0% |
| ! | CTTTACCTTAAATGTTGAAA+TGG | - | chr5.4:73032077-73032096 | MS.gene038386:intron | 25.0% |
| ! | GTATGTATTTCATAGGTTAT+AGG | - | chr5.4:73032274-73032293 | MS.gene038386:intron | 25.0% |
| ! | TAAATATGGTATGCTTCAAA+GGG | - | chr5.4:73031757-73031776 | MS.gene038386:CDS | 25.0% |
| ! | TAAGTTAAGGGAAAAAAAGA+AGG | + | chr5.4:73032756-73032775 | None:intergenic | 25.0% |
| ! | TAATCAACTTACATCTTAAG+TGG | - | chr5.4:73033764-73033783 | MS.gene038386:intron | 25.0% |
| ! | TCACTGAAATTAAATTTCGT+TGG | - | chr5.4:73033308-73033327 | MS.gene038386:intron | 25.0% |
| ! | TCTAACAAAATCATCAAACT+TGG | + | chr5.4:73033688-73033707 | None:intergenic | 25.0% |
| ! | TGAGGTAGTTAAAATAGAAA+TGG | - | chr5.4:73033428-73033447 | MS.gene038386:CDS | 25.0% |
| ! | TGATGGAAAATTATTATGTG+GGG | - | chr5.4:73032593-73032612 | MS.gene038386:intron | 25.0% |
| ! | TTAAATATGGTATGCTTCAA+AGG | - | chr5.4:73031756-73031775 | MS.gene038386:CDS | 25.0% |
| ! | TTATTATGTGGGGTTTAATT+AGG | - | chr5.4:73032603-73032622 | MS.gene038386:intron | 25.0% |
| !! | AAAAAGTAGTTTTCCCAATA+TGG | + | chr5.4:73032039-73032058 | None:intergenic | 25.0% |
| !! | ATATGTTTGATTAGAAGTGT+CGG | + | chr5.4:73032161-73032180 | None:intergenic | 25.0% |
| !! | CTCGTATTTCATTTTGTATA+TGG | - | chr5.4:73032421-73032440 | MS.gene038386:intron | 25.0% |
| !! | GAAGGAAACATTTTAAATAG+AGG | - | chr5.4:73032878-73032897 | MS.gene038386:intron | 25.0% |
| !! | GTAAGTGACATTAAATTTTC+AGG | - | chr5.4:73033513-73033532 | MS.gene038386:intron | 25.0% |
| !! | GTTCATTTGGAATAAACTTA+TGG | - | chr5.4:73031805-73031824 | MS.gene038386:CDS | 25.0% |
| !! | TTTCCCTTAACTTAATTTTG+AGG | - | chr5.4:73032762-73032781 | MS.gene038386:intron | 25.0% |
| !!! | AACCTTAAGATTAGTTTGTT+GGG | - | chr5.4:73032007-73032026 | MS.gene038386:intron | 25.0% |
| !!! | AATAAAAAGCATTTGGAAGT+TGG | + | chr5.4:73033365-73033384 | None:intergenic | 25.0% |
| !!! | ATCTTAAGTGGATTCAATTT+TGG | - | chr5.4:73033776-73033795 | MS.gene038386:intron | 25.0% |
| !!! | CTAAATTTTGAGGTTCAAAT+TGG | + | chr5.4:73033100-73033119 | None:intergenic | 25.0% |
| !!! | GTCCTTTTAACTAAATTTTG+AGG | + | chr5.4:73033110-73033129 | None:intergenic | 25.0% |
| !!! | GTGTGAAAATAAAAAGCATT+TGG | + | chr5.4:73033372-73033391 | None:intergenic | 25.0% |
| !!! | TATTGGGAAAACTACTTTTT+TGG | - | chr5.4:73032039-73032058 | MS.gene038386:intron | 25.0% |
| !!! | TCTTAAGTGGATTCAATTTT+GGG | - | chr5.4:73033777-73033796 | MS.gene038386:intron | 25.0% |
| !!! | TTCATTGCTTTTCTTTTTGT+GGG | - | chr5.4:73033844-73033863 | MS.gene038386:CDS | 25.0% |
| AAACTTGGTTCCTATTAGAA+TGG | + | chr5.4:73033673-73033692 | None:intergenic | 30.0% | |
| AAATTTCTTTCTATCGTCCA+AGG | + | chr5.4:73034084-73034103 | None:intergenic | 30.0% | |
| AACAACAGCATACCAATAAT+GGG | - | chr5.4:73031960-73031979 | MS.gene038386:intron | 30.0% | |
| AACTTGGTTCCTATTAGAAT+GGG | + | chr5.4:73033672-73033691 | None:intergenic | 30.0% | |
| AATTGTCCATTGTAAATCTG+GGG | + | chr5.4:73033715-73033734 | None:intergenic | 30.0% | |
| ACAATTGTCCATTGTAAATC+TGG | + | chr5.4:73033717-73033736 | None:intergenic | 30.0% | |
| ACTTTAATCAACATGCATGT+AGG | + | chr5.4:73033804-73033823 | None:intergenic | 30.0% | |
| ATCTGGTGTATGTATTTCAT+AGG | - | chr5.4:73032267-73032286 | MS.gene038386:intron | 30.0% | |
| ATGACCCATTTCAACATTTA+AGG | + | chr5.4:73032085-73032104 | None:intergenic | 30.0% | |
| CAATTGTCCATTGTAAATCT+GGG | + | chr5.4:73033716-73033735 | None:intergenic | 30.0% | |
| GACAAAACTTTGTATGTTCA+AGG | - | chr5.4:73032396-73032415 | MS.gene038386:intron | 30.0% | |
| TCACTATGTATTCGAGAAAA+AGG | - | chr5.4:73033044-73033063 | MS.gene038386:intron | 30.0% | |
| TCGAGAAAAAGGACTAATAT+GGG | - | chr5.4:73033055-73033074 | MS.gene038386:intron | 30.0% | |
| TCTGCAAATTGAATGTAACA+TGG | + | chr5.4:73033641-73033660 | None:intergenic | 30.0% | |
| TGTTGCACTTGAAAAGAAAA+GGG | + | chr5.4:73034004-73034023 | None:intergenic | 30.0% | |
| TTCGAGAAAAAGGACTAATA+TGG | - | chr5.4:73033054-73033073 | MS.gene038386:intron | 30.0% | |
| ! | ATTTCGGCTTACTTATTTCT+TGG | + | chr5.4:73033132-73033151 | None:intergenic | 30.0% |
| ! | CAACTACTAGTTTATAGTGA+AGG | - | chr5.4:73032997-73033016 | MS.gene038386:intron | 30.0% |
| ! | CTTTTGTTGGTCAGTTTTTT+AGG | - | chr5.4:73031783-73031802 | MS.gene038386:CDS | 30.0% |
| !! | AAACTTATGGTTTGTTCTGT+TGG | - | chr5.4:73031818-73031837 | MS.gene038386:CDS | 30.0% |
| !! | CTTAAGTGGATTCAATTTTG+GGG | - | chr5.4:73033778-73033797 | MS.gene038386:intron | 30.0% |
| !! | GAACCTTAAGATTAGTTTGT+TGG | - | chr5.4:73032006-73032025 | MS.gene038386:intron | 30.0% |
| !! | GTTCAGGTTGATGTTAAATA+TGG | - | chr5.4:73031743-73031762 | MS.gene038386:CDS | 30.0% |
| !! | GTTCATTGCTTTTCTTTTTG+TGG | - | chr5.4:73033843-73033862 | MS.gene038386:CDS | 30.0% |
| !!! | AATTTTGAGGTTCAAATTGG+TGG | + | chr5.4:73033097-73033116 | None:intergenic | 30.0% |
| !!! | CATTTTAGTGTTGACTATGT+TGG | - | chr5.4:73032116-73032135 | MS.gene038386:CDS | 30.0% |
| !!! | GCATTTTAAAACCTATGCTT+TGG | - | chr5.4:73032949-73032968 | MS.gene038386:intron | 30.0% |
| !!! | GTCAGTTTTTTAGGTTCATT+TGG | - | chr5.4:73031792-73031811 | MS.gene038386:CDS | 30.0% |
| !!! | TTTTTTCAGATCAAGTACGT+AGG | - | chr5.4:73032324-73032343 | MS.gene038386:intron | 30.0% |
| ACAATGGACAATTGTAACAC+AGG | - | chr5.4:73033722-73033741 | MS.gene038386:intron | 35.0% | |
| ACTGCAATTCCCATTCTAAT+AGG | - | chr5.4:73033660-73033679 | MS.gene038386:intron | 35.0% | |
| AGAAAAGAGGAGTTTGCAAA+TGG | - | chr5.4:73032356-73032375 | MS.gene038386:intron | 35.0% | |
| AGTTGGAACTACACTTTACT+TGG | + | chr5.4:73033348-73033367 | None:intergenic | 35.0% | |
| ATCTGACTCATACCCATTAT+TGG | + | chr5.4:73031975-73031994 | None:intergenic | 35.0% | |
| ATTAGGTGACAAAAAGTCCT+TGG | - | chr5.4:73032620-73032639 | MS.gene038386:intron | 35.0% | |
| ATTGTCCATTGTAAATCTGG+GGG | + | chr5.4:73033714-73033733 | None:intergenic | 35.0% | |
| CACCCAACAAACTAATCTTA+AGG | + | chr5.4:73032012-73032031 | None:intergenic | 35.0% | |
| GAACAACAGCATACCAATAA+TGG | - | chr5.4:73031959-73031978 | MS.gene038386:intron | 35.0% | |
| GACTAATATGGGACTAATCT+GGG | - | chr5.4:73033066-73033085 | MS.gene038386:intron | 35.0% | |
| GACTTGTACCTTTCATATAC+AGG | - | chr5.4:73033948-73033967 | MS.gene038386:CDS | 35.0% | |
| GGTCTTTGAATGTTTGAATC+TGG | - | chr5.4:73032250-73032269 | MS.gene038386:intron | 35.0% | |
| GTATGAGTCAGATTCTGATT+TGG | - | chr5.4:73031982-73032001 | MS.gene038386:intron | 35.0% | |
| GTGTTGCACTTGAAAAGAAA+AGG | + | chr5.4:73034005-73034024 | None:intergenic | 35.0% | |
| TACCTTTCATATACAGGCAA+GGG | - | chr5.4:73033954-73033973 | MS.gene038386:CDS | 35.0% | |
| TAGGAAATGAGGTAGAAAAG+AGG | - | chr5.4:73032343-73032362 | MS.gene038386:intron | 35.0% | |
| TGAAACTGTAGTAGCCTTTA+TGG | - | chr5.4:73033480-73033499 | MS.gene038386:CDS | 35.0% | |
| TTACCACTTATCAGCCATAA+AGG | + | chr5.4:73033497-73033516 | None:intergenic | 35.0% | |
| ! | TTTTGTATATGGGATGTTGC+AGG | - | chr5.4:73032432-73032451 | MS.gene038386:intron | 35.0% |
| !! | CTCTAACATGGAGTTGATAT+GGG | - | chr5.4:73032856-73032875 | MS.gene038386:intron | 35.0% |
| !!! | CTTCAAAGGGTATCTTTTGT+TGG | - | chr5.4:73031770-73031789 | MS.gene038386:CDS | 35.0% |
| AACCTTCTTCTCCAAAGCAT+AGG | + | chr5.4:73032963-73032982 | None:intergenic | 40.0% | |
| AACTGCATCTTTACAAGCCA+TGG | + | chr5.4:73032655-73032674 | None:intergenic | 40.0% | |
| AAGAGGAGTTTGCAAATGGA+AGG | - | chr5.4:73032360-73032379 | MS.gene038386:intron | 40.0% | |
| ACTCTGCAGGAAAATTGTTC+AGG | - | chr5.4:73031727-73031746 | MS.gene038386:CDS | 40.0% | |
| ACTGCATCTTTACAAGCCAT+GGG | + | chr5.4:73032654-73032673 | None:intergenic | 40.0% | |
| GATCAAGTACGTAGGAAATG+AGG | - | chr5.4:73032332-73032351 | MS.gene038386:intron | 40.0% | |
| GGACTAATATGGGACTAATC+TGG | - | chr5.4:73033065-73033084 | MS.gene038386:intron | 40.0% | |
| GTACCTTTCATATACAGGCA+AGG | - | chr5.4:73033953-73033972 | MS.gene038386:CDS | 40.0% | |
| TAATATGGGACTAATCTGGG+TGG | - | chr5.4:73033069-73033088 | MS.gene038386:intron | 40.0% | |
| TAGCCTTTATGGCTGATAAG+TGG | - | chr5.4:73033491-73033510 | MS.gene038386:CDS | 40.0% | |
| TGAAGCAAGAAAGTGGAATC+AGG | - | chr5.4:73033264-73033283 | MS.gene038386:intron | 40.0% | |
| TGGAACTACACTTTACTTGG+TGG | + | chr5.4:73033345-73033364 | None:intergenic | 40.0% | |
| TGTTGGGTGATTGCCATATT+GGG | - | chr5.4:73032023-73032042 | MS.gene038386:intron | 40.0% | |
| TTGTTGGGTGATTGCCATAT+TGG | - | chr5.4:73032022-73032041 | MS.gene038386:intron | 40.0% | |
| !! | AACATGGAGTTGATATGGGA+AGG | - | chr5.4:73032860-73032879 | MS.gene038386:intron | 40.0% |
| !! | AACCTATGCTTTGGAGAAGA+AGG | - | chr5.4:73032958-73032977 | MS.gene038386:intron | 40.0% |
| !! | ATTCAAAGGCGTCTCAATGT+CGG | + | chr5.4:73032210-73032229 | None:intergenic | 40.0% |
| !! | CAAAGCTCTTTAACTTGCCT+TGG | - | chr5.4:73034064-73034083 | MS.gene038386:CDS | 40.0% |
| !! | CTGTGTTTTTGCAGTGTTGT+TGG | - | chr5.4:73033232-73033251 | MS.gene038386:intron | 40.0% |
| !! | GCTCTAACATGGAGTTGATA+TGG | - | chr5.4:73032855-73032874 | MS.gene038386:intron | 40.0% |
| !! | GTTCATATGTGGCTCTAACA+TGG | - | chr5.4:73032844-73032863 | MS.gene038386:intron | 40.0% |
| !! | GTTTTTGCAGTGTTGTTGGA+TGG | - | chr5.4:73033236-73033255 | MS.gene038386:intron | 40.0% |
| AAACCATCATCAACCGTAGC+CGG | + | chr5.4:73031932-73031951 | None:intergenic | 45.0% | |
| AGCCATGGGAATTTGATCCA+AGG | + | chr5.4:73032640-73032659 | None:intergenic | 45.0% | |
| AGCCCTTGCCTGTATATGAA+AGG | + | chr5.4:73033959-73033978 | None:intergenic | 45.0% | |
| GACTTCCCCCAGATTTACAA+TGG | - | chr5.4:73033706-73033725 | MS.gene038386:intron | 45.0% | |
| GGTACAGTGAAGCAAGAAAG+TGG | - | chr5.4:73033257-73033276 | MS.gene038386:intron | 45.0% | |
| GTCCTTGGATCAAATTCCCA+TGG | - | chr5.4:73032635-73032654 | MS.gene038386:intron | 45.0% | |
| ACAGGCAAGGGCTTATGCAA+GGG | - | chr5.4:73033966-73033985 | MS.gene038386:CDS | 50.0% | |
| GGCTTATGCAAGGGCAATGA+AGG | - | chr5.4:73033975-73033994 | MS.gene038386:CDS | 50.0% | |
| TACAGGCAAGGGCTTATGCA+AGG | - | chr5.4:73033965-73033984 | MS.gene038386:CDS | 50.0% | |
| TCATCAACCGTAGCCGGAGA+CGG | + | chr5.4:73031926-73031945 | None:intergenic | 55.0% | |
| !! | TCTCCGGCTACGGTTGATGA+TGG | - | chr5.4:73031926-73031945 | MS.gene038386:intron | 55.0% |
| TCAACCGTAGCCGGAGACGG+CGG | + | chr5.4:73031923-73031942 | None:intergenic | 65.0% | |
| GCCGGAGACGGCGGCGACAA+CGG | + | chr5.4:73031914-73031933 | None:intergenic | 75.0% | |
| GCCGTTGTCGCCGCCGTCTC+CGG | - | chr5.4:73031910-73031929 | MS.gene038386:intron | 75.0% | |
| GTCGCCGCCGTCTCCGGCTA+CGG | - | chr5.4:73031916-73031935 | MS.gene038386:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 73031719 | 73034134 | 73031719 | ID=MS.gene038386 |
| chr5.4 | mRNA | 73031719 | 73034134 | 73031719 | ID=MS.gene038386.t1;Parent=MS.gene038386 |
| chr5.4 | exon | 73033793 | 73034134 | 73033793 | ID=MS.gene038386.t1.exon1;Parent=MS.gene038386.t1 |
| chr5.4 | CDS | 73033793 | 73034134 | 73033793 | ID=cds.MS.gene038386.t1;Parent=MS.gene038386.t1 |
| chr5.4 | exon | 73033400 | 73033520 | 73033400 | ID=MS.gene038386.t1.exon2;Parent=MS.gene038386.t1 |
| chr5.4 | CDS | 73033400 | 73033520 | 73033400 | ID=cds.MS.gene038386.t1;Parent=MS.gene038386.t1 |
| chr5.4 | exon | 73033132 | 73033228 | 73033132 | ID=MS.gene038386.t1.exon3;Parent=MS.gene038386.t1 |
| chr5.4 | CDS | 73033132 | 73033228 | 73033132 | ID=cds.MS.gene038386.t1;Parent=MS.gene038386.t1 |
| chr5.4 | exon | 73032568 | 73032607 | 73032568 | ID=MS.gene038386.t1.exon4;Parent=MS.gene038386.t1 |
| chr5.4 | CDS | 73032568 | 73032607 | 73032568 | ID=cds.MS.gene038386.t1;Parent=MS.gene038386.t1 |
| chr5.4 | exon | 73032110 | 73032193 | 73032110 | ID=MS.gene038386.t1.exon5;Parent=MS.gene038386.t1 |
| chr5.4 | CDS | 73032110 | 73032193 | 73032110 | ID=cds.MS.gene038386.t1;Parent=MS.gene038386.t1 |
| chr5.4 | exon | 73031719 | 73031883 | 73031719 | ID=MS.gene038386.t1.exon6;Parent=MS.gene038386.t1 |
| chr5.4 | CDS | 73031719 | 73031883 | 73031719 | ID=cds.MS.gene038386.t1;Parent=MS.gene038386.t1 |
| Gene Sequence |
| Protein sequence |
