Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038691.t1 | XP_003628618.1 | 98.7 | 395 | 4 | 1 | 1 | 394 | 1 | 395 | 4.60E-223 | 783.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038691.t1 | P52902 | 82.5 | 377 | 66 | 0 | 18 | 394 | 21 | 397 | 3.3e-185 | 649.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038691.t1 | G7LEP9 | 98.7 | 395 | 4 | 1 | 1 | 394 | 1 | 395 | 3.3e-223 | 783.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene038691 | MS.gene049277 | PPI |
| MS.gene038691 | MS.gene06396 | PPI |
| MS.gene038691 | MS.gene074258 | PPI |
| MS.gene038691 | MS.gene054963 | PPI |
| MS.gene038691 | MS.gene76034 | PPI |
| MS.gene038691 | MS.gene013304 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038691.t1 | MTR_8g062670 | 98.734 | 395 | 4 | 1 | 1 | 394 | 1 | 395 | 0.0 | 816 |
| MS.gene038691.t1 | MTR_5g036600 | 82.984 | 382 | 63 | 1 | 13 | 394 | 16 | 395 | 0.0 | 654 |
| MS.gene038691.t1 | MTR_8g024310 | 35.629 | 421 | 226 | 9 | 6 | 388 | 3 | 416 | 2.86e-81 | 256 |
| MS.gene038691.t1 | MTR_3g088845 | 28.923 | 325 | 221 | 4 | 57 | 375 | 31 | 351 | 5.31e-42 | 152 |
| MS.gene038691.t1 | MTR_3g088845 | 28.923 | 325 | 221 | 4 | 57 | 375 | 119 | 439 | 2.14e-41 | 152 |
| MS.gene038691.t1 | MTR_3g096390 | 28.698 | 338 | 219 | 7 | 49 | 373 | 90 | 418 | 4.29e-40 | 148 |
| MS.gene038691.t1 | MTR_3g096390 | 27.668 | 253 | 161 | 7 | 49 | 288 | 90 | 333 | 1.30e-23 | 100 |
| MS.gene038691.t1 | MTR_3g088845 | 25.137 | 183 | 130 | 2 | 57 | 236 | 119 | 297 | 1.35e-16 | 80.5 |
| MS.gene038691.t1 | MTR_7g118200 | 38.095 | 84 | 47 | 3 | 223 | 301 | 5 | 88 | 2.01e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038691.t1 | AT1G24180 | 77.582 | 397 | 82 | 4 | 1 | 394 | 1 | 393 | 0.0 | 645 |
| MS.gene038691.t1 | AT1G59900 | 76.142 | 394 | 89 | 3 | 1 | 394 | 1 | 389 | 0.0 | 643 |
| MS.gene038691.t1 | AT1G59900 | 76.316 | 380 | 85 | 3 | 1 | 380 | 1 | 375 | 0.0 | 623 |
| MS.gene038691.t1 | AT1G01090 | 37.306 | 386 | 221 | 9 | 20 | 388 | 36 | 417 | 6.88e-77 | 244 |
| MS.gene038691.t1 | AT5G09300 | 28.097 | 331 | 218 | 6 | 54 | 373 | 49 | 370 | 2.42e-41 | 150 |
| MS.gene038691.t1 | AT5G09300 | 28.097 | 331 | 218 | 6 | 54 | 373 | 120 | 441 | 6.31e-41 | 150 |
| MS.gene038691.t1 | AT1G21400 | 30.820 | 305 | 197 | 5 | 99 | 394 | 72 | 371 | 6.12e-40 | 146 |
| MS.gene038691.t1 | AT1G21400 | 30.820 | 305 | 197 | 5 | 99 | 394 | 72 | 371 | 6.12e-40 | 146 |
| MS.gene038691.t1 | AT1G21400 | 30.820 | 305 | 197 | 5 | 99 | 394 | 89 | 388 | 1.12e-39 | 146 |
| MS.gene038691.t1 | AT1G21400 | 30.820 | 305 | 197 | 5 | 99 | 394 | 164 | 463 | 1.51e-39 | 147 |
| MS.gene038691.t1 | AT1G21400 | 30.820 | 305 | 197 | 5 | 99 | 394 | 216 | 515 | 1.96e-39 | 147 |
| MS.gene038691.t1 | AT5G09300 | 27.530 | 247 | 159 | 6 | 54 | 289 | 120 | 357 | 1.54e-24 | 103 |
| MS.gene038691.t1 | AT5G34780 | 34.043 | 188 | 109 | 5 | 194 | 373 | 31 | 211 | 9.63e-22 | 95.9 |
| MS.gene038691.t1 | AT5G09300 | 26.882 | 186 | 129 | 2 | 54 | 236 | 120 | 301 | 2.37e-19 | 89.0 |
| MS.gene038691.t1 | AT1G21400 | 27.703 | 148 | 104 | 1 | 99 | 243 | 216 | 363 | 2.60e-16 | 80.1 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGATCTTCCTGCTATTT+TGG | 0.127087 | 8.4:-38548319 | MS.gene038691:CDS |
| TTTACCAATGTATATGTTAA+AGG | 0.140584 | 8.4:-38541114 | MS.gene038691:CDS |
| CTTGGAGTTGGATTGGCTTT+TGG | 0.188485 | 8.4:-38548441 | MS.gene038691:CDS |
| TTCTACTGAAAAGGAATTAA+AGG | 0.202760 | 8.4:-38542432 | MS.gene038691:intron |
| TCGTATTGAGGAAAGGAAAT+AGG | 0.238108 | 8.4:+38548920 | None:intergenic |
| CTCTTAATATCGCCGCTCTT+TGG | 0.242363 | 8.4:-38548338 | MS.gene038691:CDS |
| TGGTGATGGTGCTGCTAATC+AGG | 0.297061 | 8.4:-38548376 | MS.gene038691:CDS |
| GGTTTCGACGGTTAGGGTTT+CGG | 0.303370 | 8.4:+38548872 | None:intergenic |
| AAGGAAAGATGGGTGTTCTA+AGG | 0.319128 | 8.4:-38548544 | MS.gene038691:CDS |
| GGATGGAGGTTTCGACGGTT+AGG | 0.337250 | 8.4:+38548865 | None:intergenic |
| AATGTATATGTTAAAGGCTT+AGG | 0.339639 | 8.4:-38541108 | MS.gene038691:CDS |
| TCTTAATATCGCCGCTCTTT+GGG | 0.347641 | 8.4:-38548337 | MS.gene038691:CDS |
| AGCTTTCTTACTCTCTCAAT+TGG | 0.359233 | 8.4:+38542473 | None:intergenic |
| GCAGAAACCGCGGATGAGTT+TGG | 0.369250 | 8.4:+38548713 | None:intergenic |
| ATTTACTTTGTACAGGTAGA+TGG | 0.383832 | 8.4:-38546563 | MS.gene038691:intron |
| ATGTATATGTTAAAGGCTTA+GGG | 0.394667 | 8.4:-38541107 | MS.gene038691:CDS |
| GTTGGAGCTCAGATTCCTCT+TGG | 0.398329 | 8.4:-38548459 | MS.gene038691:CDS |
| GATTCCTCTTGGAGTTGGAT+TGG | 0.401098 | 8.4:-38548448 | MS.gene038691:CDS |
| TGGCCACTCCATGTCTGATC+CGG | 0.401479 | 8.4:-38542641 | MS.gene038691:CDS |
| CTTGTAATACGCAGGACTTT+TGG | 0.405631 | 8.4:+38548152 | None:intergenic |
| TAATGTCCAGATTCTTGAAA+TGG | 0.407066 | 8.4:-38542680 | MS.gene038691:intron |
| TATGGTGGACATGGGATTGT+TGG | 0.408224 | 8.4:-38548477 | MS.gene038691:CDS |
| TGGAAGGTGGTTCGCAGTTG+TGG | 0.409089 | 8.4:+38548832 | None:intergenic |
| ATATGGTTATACCTTTGCTT+TGG | 0.412121 | 8.4:+38541269 | None:intergenic |
| GCTCAGATTCCTCTTGGAGT+TGG | 0.414889 | 8.4:-38548453 | MS.gene038691:CDS |
| CTTTGTACAGGTAGATGGTA+TGG | 0.416779 | 8.4:-38546558 | MS.gene038691:intron |
| CGTATTGAGGAAAGGAAATA+GGG | 0.419858 | 8.4:+38548921 | None:intergenic |
| GATGGAGGTTTCGACGGTTA+GGG | 0.431680 | 8.4:+38548866 | None:intergenic |
| GGTTAGGGTTTCGGTGGAAG+AGG | 0.446559 | 8.4:+38548881 | None:intergenic |
| TGAGTTCGGAGGCGGTGGTT+TGG | 0.454095 | 8.4:+38548802 | None:intergenic |
| GTGGTTTGGACGGTGGTGGA+AGG | 0.454242 | 8.4:+38548816 | None:intergenic |
| AAGCGTGGAGATTATGCTCC+TGG | 0.457396 | 8.4:-38548132 | MS.gene038691:CDS |
| TTCGGAGGCGGTGGTTTGGA+CGG | 0.457880 | 8.4:+38548806 | None:intergenic |
| AGATTATGCTCCTGGATTGA+AGG | 0.459060 | 8.4:-38548124 | MS.gene038691:intron |
| CTGCCATCTCTACGACGGTC+AGG | 0.461075 | 8.4:-38548694 | MS.gene038691:CDS |
| ATCTTTCTAACCTTCAATCC+AGG | 0.464660 | 8.4:+38548114 | None:intergenic |
| TTCGGTGGAAGAGGAAGAAA+TGG | 0.466080 | 8.4:+38548890 | None:intergenic |
| GGAAGGTGGTTCGCAGTTGT+GGG | 0.469466 | 8.4:+38548833 | None:intergenic |
| GGCGGTGGTTTGGACGGTGG+TGG | 0.470193 | 8.4:+38548812 | None:intergenic |
| TACAAAGCAAAAGTAACATT+GGG | 0.474957 | 8.4:+38548399 | None:intergenic |
| CGATTCTCATCCTCTCAATT+CGG | 0.478801 | 8.4:-38548960 | MS.gene038691:CDS |
| TCACGACATTTCTACTGAAA+AGG | 0.484619 | 8.4:-38542441 | MS.gene038691:CDS |
| GAATTGATGGGAAGGAAAGA+TGG | 0.489679 | 8.4:-38548555 | MS.gene038691:CDS |
| TTATACCTTTGCTTTGGCAA+TGG | 0.489696 | 8.4:+38541275 | None:intergenic |
| GAGAGTAAGAAAGCTGGTAT+TGG | 0.491864 | 8.4:-38542465 | MS.gene038691:CDS |
| CGCAGTTGTGGGAAGTGAAA+GGG | 0.491966 | 8.4:+38548844 | None:intergenic |
| ATTTGTGTAACAGATGGAAT+GGG | 0.492493 | 8.4:-38548195 | MS.gene038691:intron |
| AAGGAACATGCTTTGAAGAA+TGG | 0.493732 | 8.4:-38546500 | MS.gene038691:CDS |
| GGACATGAGTTCGGAGGCGG+TGG | 0.495990 | 8.4:+38548797 | None:intergenic |
| TGAGATTAGCGGTGTTAGAC+AGG | 0.497125 | 8.4:-38542596 | MS.gene038691:intron |
| GATTTGTGTAACAGATGGAA+TGG | 0.503553 | 8.4:-38548196 | MS.gene038691:intron |
| CGCGTGCGGTATGTGCTGCC+CGG | 0.506711 | 8.4:+38542622 | None:intergenic |
| AGGAAAGATGGGTGTTCTAA+GGG | 0.508469 | 8.4:-38548543 | MS.gene038691:CDS |
| AATGGAACGGTGTCGTATTG+AGG | 0.512155 | 8.4:+38548908 | None:intergenic |
| GAGAACCATGTCGTTGAAGA+AGG | 0.516045 | 8.4:+38548776 | None:intergenic |
| ACATTGGTAAATAGATCAGA+TGG | 0.517521 | 8.4:+38541126 | None:intergenic |
| GGCCACTCCATGTCTGATCC+GGG | 0.519101 | 8.4:-38542640 | MS.gene038691:CDS |
| TTCTCACAAACCAAAATAGC+AGG | 0.519315 | 8.4:+38548309 | None:intergenic |
| GTAAATAGATCAGATGGGTC+TGG | 0.522363 | 8.4:+38541132 | None:intergenic |
| TGCAGGCATGTGGTGCTGAT+AGG | 0.523890 | 8.4:-38540776 | MS.gene038691:intron |
| GTTGAAGAAGGACATGAGTT+CGG | 0.527595 | 8.4:+38548788 | None:intergenic |
| GGTCAGGAAGCCGTTGCTGT+TGG | 0.530115 | 8.4:-38548678 | MS.gene038691:CDS |
| GTTTGGACGGTGGTGGAAGG+TGG | 0.531937 | 8.4:+38548819 | None:intergenic |
| ATACAAAGCAAAAGTAACAT+TGG | 0.535697 | 8.4:+38548398 | None:intergenic |
| GATGGGTGTTCTAAGGGAAA+AGG | 0.535962 | 8.4:-38548537 | MS.gene038691:CDS |
| CATGGTTCTCATGCGCCGTA+TGG | 0.536252 | 8.4:-38548763 | MS.gene038691:CDS |
| GGAAGCCGTTGCTGTTGGAA+TGG | 0.541582 | 8.4:-38548673 | MS.gene038691:CDS |
| TCGCAGTTGTGGGAAGTGAA+AGG | 0.544039 | 8.4:+38548843 | None:intergenic |
| GTGGGAAGTGAAAGGGATGG+AGG | 0.555368 | 8.4:+38548851 | None:intergenic |
| TGTTAAAGGCTTAGGGGTCG+AGG | 0.557159 | 8.4:-38541100 | MS.gene038691:intron |
| GGAGGCGGTGGTTTGGACGG+TGG | 0.567848 | 8.4:+38548809 | None:intergenic |
| TAAGCCTTTAACATATACAT+TGG | 0.568328 | 8.4:+38541110 | None:intergenic |
| CTTCCTGACCGTCGTAGAGA+TGG | 0.569109 | 8.4:+38548691 | None:intergenic |
| TGCTTTGAAGAATGGTCCCC+TGG | 0.570115 | 8.4:-38546492 | MS.gene038691:intron |
| AAAGGAAATAGGGTTTGAGA+AGG | 0.572428 | 8.4:+38548931 | None:intergenic |
| CATGTCCTTCTTCAACGACA+TGG | 0.574969 | 8.4:-38548781 | MS.gene038691:CDS |
| GAAGGACATGAGTTCGGAGG+CGG | 0.576411 | 8.4:+38548794 | None:intergenic |
| AAGGAAATAGGGTTTGAGAA+GGG | 0.576534 | 8.4:+38548932 | None:intergenic |
| AGCTTCCATTCCAACAGCAA+CGG | 0.576715 | 8.4:+38548668 | None:intergenic |
| GAAAGGGATGGAGGTTTCGA+CGG | 0.581945 | 8.4:+38548860 | None:intergenic |
| GATCAGACATGGAGTGGCCA+TGG | 0.584534 | 8.4:+38542644 | None:intergenic |
| GAAATAGGGTTTGAGAAGGG+TGG | 0.590452 | 8.4:+38548935 | None:intergenic |
| TTCGACGGTTAGGGTTTCGG+TGG | 0.592878 | 8.4:+38548875 | None:intergenic |
| AATTGATGGGAAGGAAAGAT+GGG | 0.593176 | 8.4:-38548554 | MS.gene038691:CDS |
| TGTGCTGCCCGGATCAGACA+TGG | 0.594191 | 8.4:+38542633 | None:intergenic |
| CGAAGCCATTGCCAAAGCAA+AGG | 0.598171 | 8.4:-38541280 | MS.gene038691:intron |
| AATTGAGAGAGTAAGAAAGC+TGG | 0.598823 | 8.4:-38542471 | MS.gene038691:CDS |
| TGCCCGGATCAGACATGGAG+TGG | 0.600111 | 8.4:+38542638 | None:intergenic |
| GCAAGCTTGCAAATTTGCAA+AGG | 0.602106 | 8.4:-38546519 | MS.gene038691:CDS |
| GGTGATGGTGCTGCTAATCA+GGG | 0.617038 | 8.4:-38548375 | MS.gene038691:CDS |
| GGGTGTTCTAAGGGAAAAGG+TGG | 0.623922 | 8.4:-38548534 | MS.gene038691:CDS |
| AGTCAGCAGCGATCTCCATA+CGG | 0.627581 | 8.4:+38548748 | None:intergenic |
| AACGGTGTCGTATTGAGGAA+AGG | 0.627993 | 8.4:+38548913 | None:intergenic |
| AGTACAGTGATCACGATAAG+CGG | 0.629898 | 8.4:+38548617 | None:intergenic |
| CGCACGCGTGATGAGATTAG+CGG | 0.635779 | 8.4:-38542607 | MS.gene038691:CDS |
| CGCTAATCTCATCACGCGTG+CGG | 0.639390 | 8.4:+38542608 | None:intergenic |
| AAGGGTGGATCCGAATTGAG+AGG | 0.642462 | 8.4:+38548950 | None:intergenic |
| ATGGACACTTACAGATACCA+TGG | 0.644817 | 8.4:-38542661 | MS.gene038691:CDS |
| AGTCCTGCGTATTACAAGCG+TGG | 0.644994 | 8.4:-38548147 | MS.gene038691:CDS |
| AAAGCCAATCCAACTCCAAG+AGG | 0.648911 | 8.4:+38548444 | None:intergenic |
| CGTAGAGATGGCAGAAACCG+CGG | 0.660828 | 8.4:+38548703 | None:intergenic |
| CATTGGTAAATAGATCAGAT+GGG | 0.666454 | 8.4:+38541127 | None:intergenic |
| TACAAAGCCAAACTCATCCG+CGG | 0.667143 | 8.4:-38548720 | MS.gene038691:CDS |
| TGTATATGTTAAAGGCTTAG+GGG | 0.667239 | 8.4:-38541106 | MS.gene038691:CDS |
| ATGGAATGGGGACTGCTGAG+TGG | 0.670807 | 8.4:-38548182 | MS.gene038691:CDS |
| TGGAAGAGGAAGAAATGGAA+CGG | 0.671358 | 8.4:+38548895 | None:intergenic |
| TCTCCACGCTTGTAATACGC+AGG | 0.672536 | 8.4:+38548144 | None:intergenic |
| AGCAGGAAGATCCCAAAGAG+CGG | 0.675016 | 8.4:+38548326 | None:intergenic |
| GAAGAAGGACATGAGTTCGG+AGG | 0.678757 | 8.4:+38548791 | None:intergenic |
| GTTGTGGGAAGTGAAAGGGA+TGG | 0.697878 | 8.4:+38548848 | None:intergenic |
| GGTTTCTGCCATCTCTACGA+CGG | 0.718050 | 8.4:-38548699 | MS.gene038691:CDS |
| TTTGTGTAACAGATGGAATG+GGG | 0.720617 | 8.4:-38548194 | MS.gene038691:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 38540749 | 38548994 | 38540749 | ID=MS.gene038691 |
| chr8.4 | mRNA | 38540749 | 38548994 | 38540749 | ID=MS.gene038691.t1;Parent=MS.gene038691 |
| chr8.4 | exon | 38548301 | 38548994 | 38548301 | ID=MS.gene038691.t1.exon1;Parent=MS.gene038691.t1 |
| chr8.4 | CDS | 38548301 | 38548994 | 38548301 | ID=cds.MS.gene038691.t1;Parent=MS.gene038691.t1 |
| chr8.4 | exon | 38548125 | 38548204 | 38548125 | ID=MS.gene038691.t1.exon2;Parent=MS.gene038691.t1 |
| chr8.4 | CDS | 38548125 | 38548204 | 38548125 | ID=cds.MS.gene038691.t1;Parent=MS.gene038691.t1 |
| chr8.4 | exon | 38546493 | 38546570 | 38546493 | ID=MS.gene038691.t1.exon3;Parent=MS.gene038691.t1 |
| chr8.4 | CDS | 38546493 | 38546570 | 38546493 | ID=cds.MS.gene038691.t1;Parent=MS.gene038691.t1 |
| chr8.4 | exon | 38542597 | 38542692 | 38542597 | ID=MS.gene038691.t1.exon4;Parent=MS.gene038691.t1 |
| chr8.4 | CDS | 38542597 | 38542692 | 38542597 | ID=cds.MS.gene038691.t1;Parent=MS.gene038691.t1 |
| chr8.4 | exon | 38542433 | 38542504 | 38542433 | ID=MS.gene038691.t1.exon5;Parent=MS.gene038691.t1 |
| chr8.4 | CDS | 38542433 | 38542504 | 38542433 | ID=cds.MS.gene038691.t1;Parent=MS.gene038691.t1 |
| chr8.4 | exon | 38541281 | 38541334 | 38541281 | ID=MS.gene038691.t1.exon6;Parent=MS.gene038691.t1 |
| chr8.4 | CDS | 38541281 | 38541334 | 38541281 | ID=cds.MS.gene038691.t1;Parent=MS.gene038691.t1 |
| chr8.4 | exon | 38541101 | 38541166 | 38541101 | ID=MS.gene038691.t1.exon7;Parent=MS.gene038691.t1 |
| chr8.4 | CDS | 38541101 | 38541166 | 38541101 | ID=cds.MS.gene038691.t1;Parent=MS.gene038691.t1 |
| chr8.4 | exon | 38540749 | 38540793 | 38540749 | ID=MS.gene038691.t1.exon8;Parent=MS.gene038691.t1 |
| chr8.4 | CDS | 38540749 | 38540793 | 38540749 | ID=cds.MS.gene038691.t1;Parent=MS.gene038691.t1 |
| Gene Sequence |
| Protein sequence |
