Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03874.t1 | XP_013455799.1 | 98.9 | 564 | 6 | 0 | 1 | 564 | 1 | 564 | 0.00E+00 | 1106.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03874.t1 | Q8VCM8 | 33.2 | 539 | 304 | 15 | 20 | 532 | 28 | 536 | 1.8e-67 | 258.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03874.t1 | A0A072UVE5 | 98.9 | 564 | 6 | 0 | 1 | 564 | 1 | 564 | 0.0e+00 | 1106.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene07068 | MS.gene03874 | PPI |
| MS.gene072688 | MS.gene03874 | PPI |
| MS.gene28821 | MS.gene03874 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03874.t1 | MTR_4g052530 | 98.936 | 564 | 6 | 0 | 1 | 564 | 1 | 564 | 0.0 | 1142 |
| MS.gene03874.t1 | MTR_4g052530 | 95.548 | 584 | 6 | 1 | 1 | 564 | 1 | 584 | 0.0 | 1132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03874.t1 | AT3G44330 | 74.074 | 567 | 138 | 4 | 1 | 562 | 1 | 563 | 0.0 | 853 |
Find 123 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACATTGACACCGTGTTAT+TGG | 0.199855 | 4.4:-49843596 | MS.gene03874:CDS |
| AATTGTGCTCTTCAGTTTCT+TGG | 0.209041 | 4.4:-49837710 | MS.gene03874:CDS |
| TCAAGTGTTGCAGAAGATTT+AGG | 0.221551 | 4.4:-49841203 | MS.gene03874:CDS |
| CTTTGCGGACAACAGTAATT+TGG | 0.260388 | 4.4:-49838718 | MS.gene03874:CDS |
| CACATTCTTCAGCGGTTCTT+TGG | 0.280526 | 4.4:+49844758 | None:intergenic |
| TTCTAGAATTTGTTTAATAA+AGG | 0.282297 | 4.4:+49841330 | None:intergenic |
| GGTGGAGATCCAACTTTCTT+TGG | 0.305838 | 4.4:+49843270 | None:intergenic |
| ATGATCTAATCAGTTTATTT+CGG | 0.307918 | 4.4:-49837576 | MS.gene03874:CDS |
| CCGGTGCTCTGTAGCAATTC+AGG | 0.309146 | 4.4:+49840054 | None:intergenic |
| CTCAACATGTCCTTTGTTCA+AGG | 0.340657 | 4.4:-49846416 | MS.gene03874:intron |
| TCCACCCCTTACAAATATTC+AGG | 0.348535 | 4.4:-49843253 | MS.gene03874:intron |
| TGGCATCATATGACACCTTT+GGG | 0.352355 | 4.4:-49842256 | MS.gene03874:CDS |
| GGACAATACAATCTGCTGTT+TGG | 0.356916 | 4.4:-49842040 | MS.gene03874:CDS |
| GATGAAGACGAGTGCGATTA+CGG | 0.357448 | 4.4:+49846625 | None:intergenic |
| TGTTTGCAGGGATGGTTGCC+AGG | 0.368228 | 4.4:-49842326 | MS.gene03874:intron |
| TGGTGCTACTCGAGGTGTTC+GGG | 0.368397 | 4.4:+49838653 | None:intergenic |
| GCTCATGGGAAAATGATCTA+TGG | 0.378313 | 4.4:-49841381 | MS.gene03874:CDS |
| AGCACCGGTGGTTTGGTTGA+TGG | 0.380580 | 4.4:-49840039 | MS.gene03874:CDS |
| CGGGTTCCATTGTAGTTGTA+AGG | 0.382485 | 4.4:+49842001 | None:intergenic |
| GATATCTCCGGTGTTCCGTT+TGG | 0.384689 | 4.4:-49846536 | MS.gene03874:CDS |
| GACAATACAATCTGCTGTTT+GGG | 0.385785 | 4.4:-49842039 | MS.gene03874:CDS |
| TTGTATTGTCCTCTTGTCTT+CGG | 0.385886 | 4.4:+49842052 | None:intergenic |
| ACATCTGCTGTAGGTAGCTT+GGG | 0.388643 | 4.4:-49840139 | MS.gene03874:intron |
| CTTGCTGTTGCTTCTAGTGT+TGG | 0.389075 | 4.4:-49837743 | MS.gene03874:CDS |
| GACATCTGCTGTAGGTAGCT+TGG | 0.390955 | 4.4:-49840140 | MS.gene03874:intron |
| CAAACACCTGAATATTTGTA+AGG | 0.392964 | 4.4:+49843247 | None:intergenic |
| GCTATTTGCTTAGATAGTAT+TGG | 0.394414 | 4.4:-49841403 | MS.gene03874:CDS |
| GTGGCATCATATGACACCTT+TGG | 0.400492 | 4.4:-49842257 | MS.gene03874:CDS |
| TTGCTGTTGCTTCTAGTGTT+GGG | 0.402441 | 4.4:-49837742 | MS.gene03874:CDS |
| ATTACCCACCATTGCGATAG+TGG | 0.410780 | 4.4:-49842276 | MS.gene03874:CDS |
| GCTACAGAGCACCGGTGGTT+TGG | 0.416449 | 4.4:-49840046 | MS.gene03874:CDS |
| ATCCTTCTTATATTCGATCA+TGG | 0.419096 | 4.4:-49838689 | MS.gene03874:CDS |
| CTACAACGAGAAGTGCTAGA+TGG | 0.420471 | 4.4:-49838189 | MS.gene03874:CDS |
| TCCTCAAAAGCAAAATAAAC+AGG | 0.426831 | 4.4:+49843622 | None:intergenic |
| TATTGGTGGTAGCAAGCCTC+TGG | 0.428171 | 4.4:-49844859 | MS.gene03874:CDS |
| AACAGGATGTATCACCTTAC+GGG | 0.437949 | 4.4:+49841982 | None:intergenic |
| TCCAACTTTCTTTGGTTCAG+TGG | 0.446635 | 4.4:+49843278 | None:intergenic |
| CCGGTGGTTTGGTTGATGGC+AGG | 0.449964 | 4.4:-49840035 | MS.gene03874:intron |
| ATTGGTGGTAGCAAGCCTCT+GGG | 0.454869 | 4.4:-49844858 | MS.gene03874:CDS |
| TCTTATGCTTCAGATTAACT+TGG | 0.460383 | 4.4:+49841177 | None:intergenic |
| AGCACAGCTGAACATATATC+AGG | 0.467914 | 4.4:-49838139 | MS.gene03874:intron |
| ATGGTGCTACTCGAGGTGTT+CGG | 0.469594 | 4.4:+49838652 | None:intergenic |
| CTTAGATAGTATTGGCTCAT+GGG | 0.470218 | 4.4:-49841395 | MS.gene03874:CDS |
| CTGCTGTTTGGGCTAACGTC+TGG | 0.478814 | 4.4:-49842028 | MS.gene03874:CDS |
| TGGTGGTAGCAAGCCTCTGG+GGG | 0.481056 | 4.4:-49844856 | MS.gene03874:CDS |
| GCTTAGATAGTATTGGCTCA+TGG | 0.482039 | 4.4:-49841396 | MS.gene03874:CDS |
| TGACACCTTTGGGGCTGCTC+CGG | 0.485813 | 4.4:-49842246 | MS.gene03874:intron |
| CGGAGATATCGTATTGGATA+AGG | 0.488170 | 4.4:+49846549 | None:intergenic |
| GCCACTGAACCAAAGAAAGT+TGG | 0.489173 | 4.4:-49843279 | MS.gene03874:CDS |
| AAATTTACTCACAGGTAAGT+TGG | 0.502805 | 4.4:+49844704 | None:intergenic |
| CTGTTTGGGCTAACGTCTGG+TGG | 0.508872 | 4.4:-49842025 | MS.gene03874:CDS |
| AGTACGAGAGAGATCAGCAC+TGG | 0.516095 | 4.4:+49846463 | None:intergenic |
| TCACCATCTGTCTTCAGCCC+TGG | 0.516595 | 4.4:+49842308 | None:intergenic |
| AACCATGATCGAATATAAGA+AGG | 0.520778 | 4.4:+49838687 | None:intergenic |
| CCGCCAGTAGTTGCAGTAGC+AGG | 0.521090 | 4.4:+49843544 | None:intergenic |
| AAGCATATTTATGGGCATCA+AGG | 0.529020 | 4.4:-49838756 | MS.gene03874:CDS |
| CAGCCTGCTACTGCAACTAC+TGG | 0.535144 | 4.4:-49843547 | MS.gene03874:CDS |
| TACAACGAGAAGTGCTAGAT+GGG | 0.535738 | 4.4:-49838188 | MS.gene03874:CDS |
| GCGAGTAGTAATCATGATGA+TGG | 0.542254 | 4.4:-49844783 | MS.gene03874:CDS |
| ACTTGTACGACAGAGTATAT+TGG | 0.551265 | 4.4:-49844876 | MS.gene03874:intron |
| TCGTTGTAGATTAACTTCAT+CGG | 0.552366 | 4.4:+49838203 | None:intergenic |
| ACCTGAATATTTGTAAGGGG+TGG | 0.552902 | 4.4:+49843252 | None:intergenic |
| AAAGAACATACAGATCTTTG+CGG | 0.553176 | 4.4:-49838733 | MS.gene03874:CDS |
| CGGAGTCGAAGAGGATTTCG+CGG | 0.558974 | 4.4:+49846645 | None:intergenic |
| ATCAAGAAGAATGATGCCTC+TGG | 0.560007 | 4.4:-49843571 | MS.gene03874:CDS |
| TGATGCCACTATCGCAATGG+TGG | 0.566504 | 4.4:+49842271 | None:intergenic |
| ATCTAATCAGTTTATTTCGG+CGG | 0.568543 | 4.4:-49837573 | MS.gene03874:CDS |
| CAGCAAGTCAAACGTGACAC+TGG | 0.568984 | 4.4:+49837759 | None:intergenic |
| AAACACCTGAATATTTGTAA+GGG | 0.570541 | 4.4:+49843248 | None:intergenic |
| CTTATCCAATACGATATCTC+CGG | 0.573897 | 4.4:-49846548 | MS.gene03874:CDS |
| ACGAGATCCAAACGGAACAC+CGG | 0.575932 | 4.4:+49846529 | None:intergenic |
| TATTCGAATCCGAAGACAAG+AGG | 0.577516 | 4.4:-49842061 | MS.gene03874:CDS |
| CTGCTACTGCAACTACTGGC+GGG | 0.584909 | 4.4:-49843543 | MS.gene03874:intron |
| AAGAGGATTTCGCGGTTGCG+AGG | 0.587745 | 4.4:+49846653 | None:intergenic |
| AATTATCGAACCTTGAACAA+AGG | 0.589660 | 4.4:+49846406 | None:intergenic |
| GTTTGCAGGGATGGTTGCCA+GGG | 0.589899 | 4.4:-49842325 | MS.gene03874:intron |
| CGAGTAGTAATCATGATGAT+GGG | 0.590493 | 4.4:-49844782 | MS.gene03874:CDS |
| GGAAGCGATAGCAATGGAAG+TGG | 0.595409 | 4.4:-49842127 | MS.gene03874:CDS |
| TATACTTACCGCAAGAGACT+CGG | 0.595460 | 4.4:+49839891 | None:intergenic |
| GACCCTCTCTGAACTCTCTA+CGG | 0.595807 | 4.4:-49840079 | MS.gene03874:CDS |
| CAACTACAATGGAACCCGTA+AGG | 0.596945 | 4.4:-49841996 | MS.gene03874:intron |
| GAACACCGGAGATATCGTAT+TGG | 0.602447 | 4.4:+49846543 | None:intergenic |
| ATATATGTTCAGCTGTGCTG+TGG | 0.602643 | 4.4:+49838143 | None:intergenic |
| TGTTTGGGCTAACGTCTGGT+GGG | 0.605689 | 4.4:-49842024 | MS.gene03874:CDS |
| TTGGTGGTAGCAAGCCTCTG+GGG | 0.607148 | 4.4:-49844857 | MS.gene03874:CDS |
| GTGCGATTACGGAGTCGAAG+AGG | 0.609499 | 4.4:+49846636 | None:intergenic |
| TGCAGTAGCAGGCTGACCAG+AGG | 0.611293 | 4.4:+49843555 | None:intergenic |
| GGTGGTAGCAAGCCTCTGGG+GGG | 0.611979 | 4.4:-49844855 | MS.gene03874:CDS |
| CTAGCGAGACGAGATCCAAA+CGG | 0.613160 | 4.4:+49846521 | None:intergenic |
| GCCTGCGTCGACCTCTGTGA+CGG | 0.615505 | 4.4:-49846596 | MS.gene03874:CDS |
| ACTAATTGCCGAGTCTCTTG+CGG | 0.617297 | 4.4:-49839899 | MS.gene03874:intron |
| TAATTGTAGGCGTTATCAGT+GGG | 0.618232 | 4.4:-49842148 | MS.gene03874:intron |
| GGTGGGCCTTACAACTACAA+TGG | 0.619306 | 4.4:-49842007 | MS.gene03874:CDS |
| TGCCGTAGAGAGTTCAGAGA+GGG | 0.620583 | 4.4:+49840077 | None:intergenic |
| AGAACCGCTGAAGAATGTGT+TGG | 0.621201 | 4.4:-49844754 | MS.gene03874:CDS |
| CCTGCCATCAACCAAACCAC+CGG | 0.622459 | 4.4:+49840035 | None:intergenic |
| CTTCTTGATATCCAATAACA+CGG | 0.623420 | 4.4:+49843585 | None:intergenic |
| CGAAATGAAGAGAAGCAGCA+TGG | 0.624639 | 4.4:+49846489 | None:intergenic |
| AACACCTGAATATTTGTAAG+GGG | 0.627059 | 4.4:+49843249 | None:intergenic |
| GCCGTCACAGAGGTCGACGC+AGG | 0.628037 | 4.4:+49846595 | None:intergenic |
| GTAATTGTAGGCGTTATCAG+TGG | 0.631414 | 4.4:-49842149 | MS.gene03874:intron |
| CCTGAATTGCTACAGAGCAC+CGG | 0.633915 | 4.4:-49840054 | MS.gene03874:CDS |
| ACATACCGGAGCAGCCCCAA+AGG | 0.637663 | 4.4:+49842241 | None:intergenic |
| CGACGGTGGCGCCGTCACAG+AGG | 0.638908 | 4.4:+49846585 | None:intergenic |
| GGCATCATATGACACCTTTG+GGG | 0.639014 | 4.4:-49842255 | MS.gene03874:CDS |
| CCTGCTACTGCAACTACTGG+CGG | 0.643000 | 4.4:-49843544 | MS.gene03874:CDS |
| TGTACGACAGAGTATATTGG+TGG | 0.643856 | 4.4:-49844873 | MS.gene03874:intron |
| GATGCCACTATCGCAATGGT+GGG | 0.644935 | 4.4:+49842272 | None:intergenic |
| TAGCAATGGAAGTGGCATTG+TGG | 0.650973 | 4.4:-49842119 | MS.gene03874:CDS |
| TCATCCAACACATTCTTCAG+CGG | 0.659873 | 4.4:+49844750 | None:intergenic |
| AGAGGTCGACGCAGGCGACG+AGG | 0.660068 | 4.4:+49846603 | None:intergenic |
| GAATTGCTACAGAGCACCGG+TGG | 0.660576 | 4.4:-49840051 | MS.gene03874:CDS |
| TTGCCAGGGCTGAAGACAGA+TGG | 0.664980 | 4.4:-49842311 | MS.gene03874:CDS |
| GTTTCTTGGTCATCACTACG+AGG | 0.684593 | 4.4:-49837696 | MS.gene03874:CDS |
| TTTCTTGGTCATCACTACGA+GGG | 0.687354 | 4.4:-49837695 | MS.gene03874:intron |
| AGATATCGTATTGGATAAGG+CGG | 0.692129 | 4.4:+49846552 | None:intergenic |
| GTACGAGAGAGATCAGCACT+GGG | 0.694728 | 4.4:+49846464 | None:intergenic |
| GCGGTAAACATCGACGACGG+TGG | 0.697144 | 4.4:+49846571 | None:intergenic |
| ATATGATGCCACTATCGCAA+TGG | 0.698292 | 4.4:+49842268 | None:intergenic |
| GACATGTTGAGTTCACGAAG+AGG | 0.701734 | 4.4:+49846428 | None:intergenic |
| TCAGTGGGAAGCGATAGCAA+TGG | 0.705355 | 4.4:-49842133 | MS.gene03874:CDS |
| GTGCCGTAGAGAGTTCAGAG+AGG | 0.752779 | 4.4:+49840076 | None:intergenic |
| AAGGCGGTAAACATCGACGA+CGG | 0.772440 | 4.4:+49846568 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 49837543 | 49846696 | 49837543 | ID=MS.gene03874 |
| chr4.4 | mRNA | 49837543 | 49846696 | 49837543 | ID=MS.gene03874.t1;Parent=MS.gene03874 |
| chr4.4 | exon | 49846417 | 49846696 | 49846417 | ID=MS.gene03874.t1.exon1;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49846417 | 49846696 | 49846417 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49844716 | 49844885 | 49844716 | ID=MS.gene03874.t1.exon2;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49844716 | 49844885 | 49844716 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49843544 | 49843647 | 49843544 | ID=MS.gene03874.t1.exon3;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49843544 | 49843647 | 49843544 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49843254 | 49843320 | 49843254 | ID=MS.gene03874.t1.exon4;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49843254 | 49843320 | 49843254 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49842247 | 49842339 | 49842247 | ID=MS.gene03874.t1.exon5;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49842247 | 49842339 | 49842247 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49841997 | 49842161 | 49841997 | ID=MS.gene03874.t1.exon6;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49841997 | 49842161 | 49841997 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49841330 | 49841470 | 49841330 | ID=MS.gene03874.t1.exon7;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49841330 | 49841470 | 49841330 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49841154 | 49841231 | 49841154 | ID=MS.gene03874.t1.exon8;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49841154 | 49841231 | 49841154 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49840036 | 49840148 | 49840036 | ID=MS.gene03874.t1.exon9;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49840036 | 49840148 | 49840036 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49839900 | 49839960 | 49839900 | ID=MS.gene03874.t1.exon10;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49839900 | 49839960 | 49839900 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49838611 | 49838778 | 49838611 | ID=MS.gene03874.t1.exon11;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49838611 | 49838778 | 49838611 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49838140 | 49838241 | 49838140 | ID=MS.gene03874.t1.exon12;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49838140 | 49838241 | 49838140 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49837696 | 49837785 | 49837696 | ID=MS.gene03874.t1.exon13;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49837696 | 49837785 | 49837696 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| chr4.4 | exon | 49837543 | 49837605 | 49837543 | ID=MS.gene03874.t1.exon14;Parent=MS.gene03874.t1 |
| chr4.4 | CDS | 49837543 | 49837605 | 49837543 | ID=cds.MS.gene03874.t1;Parent=MS.gene03874.t1 |
| Gene Sequence |
| Protein sequence |
