AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene039185

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039185.t1 MTR_2g070420 98.656 372 5 0 1 372 1 372 0.0 751
MS.gene039185.t1 MTR_1g072440 59.091 88 28 3 10 94 4 86 2.32e-27 103
MS.gene039185.t1 MTR_3g076640 48.649 74 35 2 4 76 4 75 7.35e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039185.t1 AT3G14420 89.315 365 39 0 3 367 2 366 0.0 666
MS.gene039185.t1 AT3G14420 89.315 365 39 0 3 367 2 366 0.0 666
MS.gene039185.t1 AT3G14420 89.041 365 40 0 3 367 2 366 0.0 663
MS.gene039185.t1 AT3G14415 88.767 365 41 0 3 367 2 366 0.0 663
MS.gene039185.t1 AT3G14415 88.767 365 41 0 3 367 2 366 0.0 663
MS.gene039185.t1 AT3G14415 87.332 371 41 1 3 367 2 372 0.0 657
MS.gene039185.t1 AT4G18360 85.596 361 52 0 3 363 2 362 0.0 651
MS.gene039185.t1 AT4G18360 85.596 361 52 0 3 363 2 362 0.0 651
MS.gene039185.t1 AT3G14420 90.202 347 34 0 21 367 1 347 0.0 635
MS.gene039185.t1 AT3G14420 90.625 320 30 0 48 367 40 359 0.0 585
MS.gene039185.t1 AT3G14420 90.625 320 30 0 48 367 46 365 0.0 583
MS.gene039185.t1 AT4G18360 84.665 313 48 0 3 315 2 314 0.0 558
MS.gene039185.t1 AT3G14130 61.972 355 132 1 1 355 1 352 7.64e-163 460
MS.gene039185.t1 AT3G14130 61.972 355 132 1 1 355 1 352 7.64e-163 460
MS.gene039185.t1 AT3G14150 60.845 355 136 1 1 355 1 352 6.28e-160 453
MS.gene039185.t1 AT3G14150 60.845 355 136 1 1 355 1 352 6.28e-160 453
MS.gene039185.t1 AT4G18360 85.714 238 34 0 3 240 2 239 5.47e-153 431
MS.gene039185.t1 AT3G14130 63.312 308 110 1 48 355 4 308 2.95e-140 401
MS.gene039185.t1 AT3G14150 60.300 267 103 1 89 355 3 266 3.94e-112 328
MS.gene039185.t1 AT3G14150 60.300 267 103 1 89 355 3 266 3.94e-112 328
MS.gene039185.t1 AT3G14150 60.300 267 103 1 89 355 3 266 3.94e-112 328
MS.gene039185.t1 AT3G14130 62.602 246 89 1 110 355 2 244 8.32e-112 327
MS.gene039185.t1 AT3G14150 60.569 246 94 1 110 355 2 244 8.84e-109 319
MS.gene039185.t1 AT3G14150 60.569 246 94 1 110 355 2 244 8.84e-109 319

Find 79 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGAAATCTTACAAAATTT+TGG 0.098632 2.1:+28287052 None:intergenic
TACCTCTTCTAAGGCACTTA+TGG 0.288795 2.1:+28284110 None:intergenic
TTCCTCATACTCACTGATAT+TGG 0.304687 2.1:+28287169 None:intergenic
TTCTTAAAGTACCTCTTCTA+AGG 0.314628 2.1:+28284101 None:intergenic
GCTTTGGCACTTGGTGCCTC+TGG 0.345429 2.1:-28283912 MS.gene039185:CDS
AGTACTCCCTTCACAAGAAT+TGG 0.346342 2.1:+28284451 None:intergenic
GTCTTCAAGGCTTTGGCACT+TGG 0.349066 2.1:-28283921 MS.gene039185:CDS
GGACTTGCTTCATATGTTGC+TGG 0.352126 2.1:-28284705 MS.gene039185:CDS
TCTAAGGCACTTATGGTTGC+TGG 0.360123 2.1:+28284117 None:intergenic
TAGTAGTCAAACGCCATCTT+TGG 0.400379 2.1:+28287128 None:intergenic
AACCGATGTCTTCAAGGCTT+TGG 0.403047 2.1:-28283928 MS.gene039185:CDS
CCGTGGAACCGATGTCTTCA+AGG 0.420956 2.1:-28283934 MS.gene039185:CDS
TGACCACATCGTTGCTGATT+GGG 0.421642 2.1:-28283245 MS.gene039185:CDS
GCAAGAGCTGCATCAGCTGC+TGG 0.425139 2.1:-28286000 MS.gene039185:CDS
GCTTCTCTGCGTCCAAGTCT+TGG 0.429282 2.1:+28285306 None:intergenic
ACTCATCACGTAACATTTGA+AGG 0.447093 2.1:+28283320 None:intergenic
TCTTTCAGACATTGTCATCA+TGG 0.448736 2.1:-28285597 MS.gene039185:intron
GTGACCACATCGTTGCTGAT+TGG 0.449542 2.1:-28283246 MS.gene039185:CDS
TCCATTTAGGCTAATGACTC+TGG 0.458314 2.1:-28284726 MS.gene039185:intron
GTGTATTCTCTGGCTGCTGA+TGG 0.460684 2.1:-28283364 MS.gene039185:CDS
GCGTTTGACTACTATGCATC+TGG 0.462223 2.1:-28287119 MS.gene039185:CDS
AACCAATATCAGTGAGTATG+AGG 0.469087 2.1:-28287171 MS.gene039185:CDS
GCAGTTGGAGCAATCATGAT+TGG 0.471140 2.1:+28286818 None:intergenic
CTTTCAGACATTGTCATCAT+GGG 0.471894 2.1:-28285596 MS.gene039185:intron
TTATTTAGGTGTACAAGGAC+AGG 0.473436 2.1:-28285401 MS.gene039185:intron
CTGGCTGCTGATGGAGAAGC+TGG 0.478038 2.1:-28283355 MS.gene039185:CDS
GCAGTGCAAAGTGGTGCAGC+TGG 0.480581 2.1:-28284183 MS.gene039185:CDS
GAAGTTGCTTCCACTGGACC+TGG 0.482888 2.1:-28285556 MS.gene039185:CDS
GTCGAAGAAGTTGCTTCCAC+TGG 0.490758 2.1:-28285562 MS.gene039185:CDS
GAGTTAACAATGGCTTTAAG+TGG 0.494236 2.1:-28283295 MS.gene039185:CDS
GTCTTGGAGTATCAACGGTA+AGG 0.498559 2.1:+28285322 None:intergenic
GTTCTTTATTTAGGTGTACA+AGG 0.502004 2.1:-28285406 MS.gene039185:intron
AGGACTGAACCTTGGAAAGA+TGG 0.503842 2.1:-28284834 MS.gene039185:CDS
GACATGTCAACTACTGTCCT+TGG 0.511523 2.1:-28286857 MS.gene039185:CDS
CTTCACAAGAATTGGAAGGC+TGG 0.519849 2.1:+28284459 None:intergenic
GAGAAGCTGATATCAAGAAC+AGG 0.524863 2.1:-28285290 MS.gene039185:intron
ATCAGCTGCTGGAACAATCA+TGG 0.526902 2.1:-28285989 MS.gene039185:intron
AAATTGATCGTACTCTGAGC+TGG 0.527284 2.1:-28284680 MS.gene039185:CDS
ACGCCCTGTGGTGTATTCTC+TGG 0.532490 2.1:-28283374 MS.gene039185:CDS
CAGCCTTCCAATTCTTGTGA+AGG 0.532608 2.1:-28284458 MS.gene039185:CDS
CATAATCTTGGTATGGCACG+AGG 0.535981 2.1:+28283205 None:intergenic
TTACTTCGTCCATCTTTCCA+AGG 0.537036 2.1:+28284825 None:intergenic
CTCACATCGATAAGAATTCG+CGG 0.537846 2.1:+28286887 None:intergenic
TGGAGAAGCTGGTGTAAGAA+AGG 0.545364 2.1:-28283344 MS.gene039185:CDS
AAGGCAGAAATTGCCAAAGA+TGG 0.545523 2.1:-28287141 MS.gene039185:CDS
AGCCTTCCAATTCTTGTGAA+GGG 0.553922 2.1:-28284457 MS.gene039185:CDS
AAGTGGATGCCGCTCACTCA+AGG 0.555841 2.1:-28283278 MS.gene039185:CDS
AATCAAGTTGTCTTGCTCCA+TGG 0.556405 2.1:+28284145 None:intergenic
CAGAAAATGGCTCATCCCGA+GGG 0.557082 2.1:-28286791 MS.gene039185:intron
AAGTTGTCTTGCTCCATGGT+TGG 0.558965 2.1:+28284149 None:intergenic
TCAGAAAATGGCTCATCCCG+AGG 0.565352 2.1:-28286792 MS.gene039185:CDS
ACCGTTGATACTCCAAGACT+TGG 0.567938 2.1:-28285318 MS.gene039185:CDS
GGAATTATTGTGTCCAACCA+TGG 0.572782 2.1:-28284162 MS.gene039185:CDS
CCTTGAAGACATCGGTTCCA+CGG 0.578507 2.1:+28283934 None:intergenic
AACCATAAGTGCCTTAGAAG+AGG 0.579700 2.1:-28284112 MS.gene039185:intron
GCAAGGCTAGCAGTGCAAAG+TGG 0.582697 2.1:-28284192 MS.gene039185:CDS
GAGTGATTTCCTTGAGTGAG+CGG 0.593581 2.1:+28283269 None:intergenic
TCTTGGAGTATCAACGGTAA+GGG 0.599818 2.1:+28285323 None:intergenic
TCCAAGTCTTGGAGTATCAA+CGG 0.600358 2.1:+28285317 None:intergenic
CATCTGGTGCTGAGGACCAG+TGG 0.600366 2.1:-28287103 MS.gene039185:CDS
TGGAGCAATCATGATTGGCA+TGG 0.601089 2.1:+28286823 None:intergenic
CTACTATGCATCTGGTGCTG+AGG 0.603217 2.1:-28287111 MS.gene039185:CDS
TAGGTTGTGAAAGCTGCTCA+AGG 0.605864 2.1:-28283990 MS.gene039185:CDS
CTCCCTTCACAAGAATTGGA+AGG 0.613725 2.1:+28284455 None:intergenic
GCAGCCAGAGAATACACCAC+AGG 0.615884 2.1:+28283370 None:intergenic
TGATGAGTTTGAGTTAACAA+TGG 0.619751 2.1:-28283305 MS.gene039185:CDS
TGATCGTACTCTGAGCTGGA+AGG 0.628034 2.1:-28284676 MS.gene039185:intron
AAAGCGGATTCCAGGTCCAG+TGG 0.630109 2.1:+28285546 None:intergenic
TTGGATGGAGGTGTACGCCG+TGG 0.653698 2.1:-28283951 MS.gene039185:CDS
CAGCCAGAGAATACACCACA+GGG 0.658781 2.1:+28283371 None:intergenic
TGCCAAAGCCTTGAAGACAT+CGG 0.666124 2.1:+28283926 None:intergenic
GTGAGTATGAGGAAATTGCA+AGG 0.666466 2.1:-28287160 MS.gene039185:CDS
TGTCCCAATCAGCAACGATG+TGG 0.667462 2.1:+28283242 None:intergenic
GAAGGGAGTACTCACTGCAG+AGG 0.669394 2.1:-28284440 MS.gene039185:CDS
TGTCTAGATTGGACGCCCTG+TGG 0.692710 2.1:-28283386 MS.gene039185:intron
GGCACGAGGATTAACACGAG+GGG 0.715789 2.1:+28283219 None:intergenic
TATACTCACAAATATGCCAG+AGG 0.718024 2.1:+28283896 None:intergenic
TGGCACGAGGATTAACACGA+GGG 0.726021 2.1:+28283218 None:intergenic
ATGGCACGAGGATTAACACG+AGG 0.730624 2.1:+28283217 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAATTATATTAATTATAG+AGG + chr2.1:28285633-28285652 None:intergenic 10.0%
!! AAATTAGTAACATAGTTTAA+TGG - chr2.1:28285602-28285621 MS.gene039185:intron 15.0%
!! AATTAATATAATTGTTACCT+TGG - chr2.1:28285637-28285656 MS.gene039185:intron 15.0%
!! CATAATAAAGTAACATAATT+TGG + chr2.1:28283387-28283406 None:intergenic 15.0%
!! TCAAATGTTTATTACAAAAT+AGG + chr2.1:28284433-28284452 None:intergenic 15.0%
!! TTAGTAGTAATTTCTTAATT+CGG - chr2.1:28286553-28286572 MS.gene039185:intron 15.0%
!!! CAATGAGAATTTAAAATATT+GGG + chr2.1:28286528-28286547 None:intergenic 15.0%
!!! GTTATAACGATAAATTATTT+TGG + chr2.1:28286938-28286957 None:intergenic 15.0%
!!! TATGAAATCTTACAAAATTT+TGG + chr2.1:28283337-28283356 None:intergenic 15.0%
!!! TTTTACGACTATTTTATTAA+TGG - chr2.1:28286153-28286172 MS.gene039185:intron 15.0%
!! AAATTCTTCAATGTCAAAAA+TGG + chr2.1:28285529-28285548 None:intergenic 20.0%
!! AATTAATTGAAGAAGTAATG+AGG + chr2.1:28284682-28284701 None:intergenic 20.0%
!! TATTAAGAAAACTCTACAAT+AGG - chr2.1:28284248-28284267 MS.gene039185:intron 20.0%
!! TATTGTTATATGTTCTGTAT+TGG - chr2.1:28284953-28284972 MS.gene039185:intron 20.0%
!! TTAAGAAATTACTACTAATG+AGG + chr2.1:28286551-28286570 None:intergenic 20.0%
!!! ATCTTACAAAATTTTGGAAA+AGG + chr2.1:28283331-28283350 None:intergenic 20.0%
!!! GCAATGAGAATTTAAAATAT+TGG + chr2.1:28286529-28286548 None:intergenic 20.0%
!!! TAATTTGTTCGTTAAGATAA+CGG + chr2.1:28286975-28286994 None:intergenic 20.0%
!!! TATTGATAACTTGATCAATA+AGG - chr2.1:28285228-28285247 MS.gene039185:intron 20.0%
! AAATTATGCAATGTCTAGAT+TGG - chr2.1:28286989-28287008 MS.gene039185:intron 25.0%
! AACTCAAAATCTAAGCATAT+TGG + chr2.1:28285448-28285467 None:intergenic 25.0%
! CAAAAACAACACTAAATCAT+TGG + chr2.1:28285868-28285887 None:intergenic 25.0%
! CAAAGAATCATATTTACATG+AGG - chr2.1:28285155-28285174 MS.gene039185:intron 25.0%
! CACTTGTCTTCTATAATATA+TGG - chr2.1:28286893-28286912 MS.gene039185:CDS 25.0%
! CATTAATAGAATTCTCAAAC+AGG + chr2.1:28285990-28286009 None:intergenic 25.0%
! GAAAATAGCTTATTACATGT+CGG - chr2.1:28284626-28284645 MS.gene039185:intron 25.0%
! TATTTGCATAGCACAATATT+TGG - chr2.1:28284594-28284613 MS.gene039185:intron 25.0%
! TCAAACGAATTTGATAAAGT+TGG + chr2.1:28286673-28286692 None:intergenic 25.0%
! TGAAAAGGAAAAACATAACA+AGG - chr2.1:28285283-28285302 MS.gene039185:intron 25.0%
! TGTTAATGTGCTGAAAAATA+TGG - chr2.1:28283453-28283472 MS.gene039185:intron 25.0%
! TTAATCTAACAGAGTTAGTT+AGG + chr2.1:28285317-28285336 None:intergenic 25.0%
! TTATCACAACTATGATAACT+TGG - chr2.1:28284096-28284115 MS.gene039185:intron 25.0%
! TTGTCTTCTATAATATATGG+AGG - chr2.1:28286896-28286915 MS.gene039185:CDS 25.0%
!! ACAAAAGAAAACACTACTTT+TGG - chr2.1:28285804-28285823 MS.gene039185:intron 25.0%
!! ACATGATAGTGTAATTTGAT+TGG - chr2.1:28284555-28284574 MS.gene039185:intron 25.0%
!! CATTGTGTTTAAGATTAACA+GGG + chr2.1:28286771-28286790 None:intergenic 25.0%
!! GTAACATAATTTGGTAACTT+AGG + chr2.1:28283378-28283397 None:intergenic 25.0%
!! TTGACATTGAAGAATTTTGA+AGG - chr2.1:28285532-28285551 MS.gene039185:intron 25.0%
!! TTTTCAATACGCTAGTATTA+GGG - chr2.1:28286056-28286075 MS.gene039185:intron 25.0%
!!! AGCATATTGGTTTTTGAAAA+AGG + chr2.1:28285435-28285454 None:intergenic 25.0%
!!! ATTTCTGAAATGGAAAATGA+AGG - chr2.1:28283648-28283667 MS.gene039185:intron 25.0%
!!! GCTTTTTCTTTAAATTCACT+TGG - chr2.1:28283625-28283644 MS.gene039185:intron 25.0%
!!! GTCATTTTTAGTCTACATAA+TGG - chr2.1:28285829-28285848 MS.gene039185:intron 25.0%
!!! TCTACATAATGGAATTTTCA+AGG - chr2.1:28285840-28285859 MS.gene039185:intron 25.0%
!!! TGTTTTTGTTAATTTTGTGC+AGG - chr2.1:28285880-28285899 MS.gene039185:intron 25.0%
!!! TTTGATAAAGTTGGCTTTTA+TGG + chr2.1:28286664-28286683 None:intergenic 25.0%
!!! TTTTTCAATACGCTAGTATT+AGG - chr2.1:28286055-28286074 MS.gene039185:intron 25.0%
ACATACGAGTTCATATGAAA+AGG - chr2.1:28285268-28285287 MS.gene039185:intron 30.0%
ACATGTCACTAAACACAAAA+GGG + chr2.1:28284332-28284351 None:intergenic 30.0%
AGAAAAAGCAATTATACCCT+CGG + chr2.1:28283614-28283633 None:intergenic 30.0%
ATATGGTTGACAAACATTAC+AGG - chr2.1:28283470-28283489 MS.gene039185:intron 30.0%
CCTGTTAATCTTAAACACAA+TGG - chr2.1:28286769-28286788 MS.gene039185:intron 30.0%
CTGAATTCATAATTCTTGAG+TGG - chr2.1:28286729-28286748 MS.gene039185:intron 30.0%
GAATTCAAGTGAAGAAGAAA+TGG + chr2.1:28284908-28284927 None:intergenic 30.0%
GTACAAAACGATAATTGAGA+CGG - chr2.1:28283856-28283875 MS.gene039185:intron 30.0%
GTACACCTAAATAAAGAACA+TGG + chr2.1:28284979-28284998 None:intergenic 30.0%
TACATGTCACTAAACACAAA+AGG + chr2.1:28284333-28284352 None:intergenic 30.0%
TCACATATAACTGGAAAAAG+CGG + chr2.1:28284859-28284878 None:intergenic 30.0%
TGTCGTAATAAAAGTTTGGT+CGG + chr2.1:28286875-28286894 None:intergenic 30.0%
TGTTATAGTGATTGCAATTG+TGG + chr2.1:28285190-28285209 None:intergenic 30.0%
TTCTTCAATGTCAAAAATGG+TGG + chr2.1:28285526-28285545 None:intergenic 30.0%
TTGACAAACATTACAGGTTT+AGG - chr2.1:28283476-28283495 MS.gene039185:intron 30.0%
! ATTCACTTGGATTTCTGAAA+TGG - chr2.1:28283638-28283657 MS.gene039185:intron 30.0%
! ATTCTGTTCATTTTGCTAGA+AGG - chr2.1:28283689-28283708 MS.gene039185:intron 30.0%
! ATTGGCCATGTTCTTTATTT+AGG - chr2.1:28284971-28284990 MS.gene039185:intron 30.0%
! CAAGTGTCGTAATAAAAGTT+TGG + chr2.1:28286879-28286898 None:intergenic 30.0%
! CCATTGTGTTTAAGATTAAC+AGG + chr2.1:28286772-28286791 None:intergenic 30.0%
! CCTGCAACATCAAAATTTTT+AGG + chr2.1:28285393-28285412 None:intergenic 30.0%
! GTTCTTTATTTAGGTGTACA+AGG - chr2.1:28284980-28284999 MS.gene039185:intron 30.0%
! TGATGAGTTTGAGTTAACAA+TGG - chr2.1:28287081-28287100 MS.gene039185:CDS 30.0%
! TTCTGTTCATTTTGCTAGAA+GGG - chr2.1:28283690-28283709 MS.gene039185:intron 30.0%
! TTCTTAAAGTACCTCTTCTA+AGG + chr2.1:28286288-28286307 None:intergenic 30.0%
! TTGATGTAAATTTTGCAGCA+AGG - chr2.1:28286177-28286196 MS.gene039185:intron 30.0%
! TTGTGTTGCTGAAATTACTT+AGG - chr2.1:28286377-28286396 MS.gene039185:intron 30.0%
! TTTTGTTGCTCACATATAAC+TGG + chr2.1:28284868-28284887 None:intergenic 30.0%
!! ATATTTACATGAGGTTGATG+CGG - chr2.1:28285164-28285183 MS.gene039185:intron 30.0%
!! CCTAAAAATTTTGATGTTGC+AGG - chr2.1:28285390-28285409 MS.gene039185:CDS 30.0%
!!! AAGCTACATTTTGAAGCTTA+TGG + chr2.1:28283952-28283971 None:intergenic 30.0%
!!! CATCTGCTAGTTTTGATAAA+TGG - chr2.1:28284201-28284220 MS.gene039185:intron 30.0%
!!! GTACAATTTTTTTCTTGTGC+AGG - chr2.1:28284350-28284369 MS.gene039185:intron 30.0%
AACCAATATCAGTGAGTATG+AGG - chr2.1:28283215-28283234 MS.gene039185:CDS 35.0%
AACGAATTGAGCGAACAAAT+TGG + chr2.1:28285743-28285762 None:intergenic 35.0%
ACTCATCACGTAACATTTGA+AGG + chr2.1:28287069-28287088 None:intergenic 35.0%
ATAGCATGTAAGTATCCAGA+AGG + chr2.1:28283775-28283794 None:intergenic 35.0%
ATCATGCATGACATGAATTG+TGG - chr2.1:28283729-28283748 MS.gene039185:intron 35.0%
ATCTCTGCAAACATCTACAT+GGG + chr2.1:28284509-28284528 None:intergenic 35.0%
ATTGTTACCTTGGTCCATTT+AGG - chr2.1:28285647-28285666 MS.gene039185:intron 35.0%
CATGTCACTAAACACAAAAG+GGG + chr2.1:28284331-28284350 None:intergenic 35.0%
CATTCATGTAGTTAACGGTT+TGG - chr2.1:28286601-28286620 MS.gene039185:intron 35.0%
CTTTCAGACATTGTCATCAT+GGG - chr2.1:28284790-28284809 MS.gene039185:intron 35.0%
GAAAAAGCAATTATACCCTC+GGG + chr2.1:28283613-28283632 None:intergenic 35.0%
GTAATTTGATTGGATGCATG+CGG - chr2.1:28284565-28284584 MS.gene039185:intron 35.0%
GTAGGTGTACAAAACATCAA+AGG + chr2.1:28283412-28283431 None:intergenic 35.0%
GTCGTAATAAAAGTTTGGTC+GGG + chr2.1:28286874-28286893 None:intergenic 35.0%
TAGCATGTAAGTATCCAGAA+GGG + chr2.1:28283774-28283793 None:intergenic 35.0%
TATACTCACAAATATGCCAG+AGG + chr2.1:28286493-28286512 None:intergenic 35.0%
TCAGTGTAATAGCTTATCAG+TGG + chr2.1:28284140-28284159 None:intergenic 35.0%
TCTTTCAGACATTGTCATCA+TGG - chr2.1:28284789-28284808 MS.gene039185:intron 35.0%
TTATTTAGGTGTACAAGGAC+AGG - chr2.1:28284985-28285004 MS.gene039185:intron 35.0%
TTCCTCATACTCACTGATAT+TGG + chr2.1:28283220-28283239 None:intergenic 35.0%
TTCTTAATTCGGATCGTCTA+TGG - chr2.1:28286564-28286583 MS.gene039185:intron 35.0%
! TAAGATAACGGCAGTACTAA+TGG + chr2.1:28286963-28286982 None:intergenic 35.0%
! TTTAGCTTATATCACCCTTC+TGG - chr2.1:28283757-28283776 MS.gene039185:intron 35.0%
!! AATTTTGAAGGACTGAACCT+TGG - chr2.1:28285544-28285563 MS.gene039185:CDS 35.0%
!! GAGTTAACAATGGCTTTAAG+TGG - chr2.1:28287091-28287110 MS.gene039185:CDS 35.0%
!! TCCAACTGCTTTTCAGAAAA+TGG - chr2.1:28283582-28283601 MS.gene039185:intron 35.0%
AAATTGATCGTACTCTGAGC+TGG - chr2.1:28285706-28285725 MS.gene039185:intron 40.0%
AACCATAAGTGCCTTAGAAG+AGG - chr2.1:28286274-28286293 MS.gene039185:intron 40.0%
AAGGCAGAAATTGCCAAAGA+TGG - chr2.1:28283245-28283264 MS.gene039185:CDS 40.0%
ACACCTCCATCCAAAAATAC+AGG + chr2.1:28286429-28286448 None:intergenic 40.0%
AGCCTTCCAATTCTTGTGAA+GGG - chr2.1:28285929-28285948 MS.gene039185:intron 40.0%
AGCTGAAAAAGCTGGATTCA+AGG - chr2.1:28285034-28285053 MS.gene039185:intron 40.0%
AGTACTCCCTTCACAAGAAT+TGG + chr2.1:28285938-28285957 None:intergenic 40.0%
CATCTCTGCAAACATCTACA+TGG + chr2.1:28284510-28284529 None:intergenic 40.0%
CGACGCATTCATGTAGTTAA+CGG - chr2.1:28286596-28286615 MS.gene039185:intron 40.0%
CTCACATCGATAAGAATTCG+CGG + chr2.1:28283502-28283521 None:intergenic 40.0%
GAGAAGCTGATATCAAGAAC+AGG - chr2.1:28285096-28285115 MS.gene039185:intron 40.0%
GGAATTATTGTGTCCAACCA+TGG - chr2.1:28286224-28286243 MS.gene039185:intron 40.0%
GTAATAAAAGTTTGGTCGGG+AGG + chr2.1:28286871-28286890 None:intergenic 40.0%
GTGAGTATGAGGAAATTGCA+AGG - chr2.1:28283226-28283245 MS.gene039185:CDS 40.0%
TAGTAGTCAAACGCCATCTT+TGG + chr2.1:28283261-28283280 None:intergenic 40.0%
TCCATTTAGGCTAATGACTC+TGG - chr2.1:28285660-28285679 MS.gene039185:intron 40.0%
TTACTTCGTCCATCTTTCCA+AGG + chr2.1:28285564-28285583 None:intergenic 40.0%
! AATCAAGTTGTCTTGCTCCA+TGG + chr2.1:28286244-28286263 None:intergenic 40.0%
! AGGTCGTGTTCCTGTATTTT+TGG - chr2.1:28286416-28286435 MS.gene039185:intron 40.0%
! ATTTTGTGCAGGATGTGAAG+TGG - chr2.1:28285891-28285910 MS.gene039185:intron 40.0%
! GTTTTTGTTCTCAGAGTCGT+AGG + chr2.1:28283430-28283449 None:intergenic 40.0%
! TCATTAGCCTAAATGGACCA+AGG + chr2.1:28285657-28285676 None:intergenic 40.0%
! TCCAAGTCTTGGAGTATCAA+CGG + chr2.1:28285072-28285091 None:intergenic 40.0%
! TCCAGAGTCATTAGCCTAAA+TGG + chr2.1:28285664-28285683 None:intergenic 40.0%
! TCTTGGAGTATCAACGGTAA+GGG + chr2.1:28285066-28285085 None:intergenic 40.0%
! TGTTTTCTTGAAGAGTCCAC+TGG + chr2.1:28283302-28283321 None:intergenic 40.0%
!! GCCATTTTCTGAAAAGCAGT+TGG + chr2.1:28283586-28283605 None:intergenic 40.0%
!! TACCTCTTCTAAGGCACTTA+TGG + chr2.1:28286279-28286298 None:intergenic 40.0%
!!! CGTGTTCCTGTATTTTTGGA+TGG - chr2.1:28286420-28286439 MS.gene039185:intron 40.0%
!!! GCATGGAAATTTTGAAGCCA+AGG + chr2.1:28283549-28283568 None:intergenic 40.0%
!!! GTTCCTGTATTTTTGGATGG+AGG - chr2.1:28286423-28286442 MS.gene039185:intron 40.0%
AACCGATGTCTTCAAGGCTT+TGG - chr2.1:28286458-28286477 MS.gene039185:intron 45.0%
AAGTTGTCTTGCTCCATGGT+TGG + chr2.1:28286240-28286259 None:intergenic 45.0%
ACCGTTGATACTCCAAGACT+TGG - chr2.1:28285068-28285087 MS.gene039185:intron 45.0%
AGGACTGAACCTTGGAAAGA+TGG - chr2.1:28285552-28285571 MS.gene039185:CDS 45.0%
ATCAGCTGCTGGAACAATCA+TGG - chr2.1:28284397-28284416 MS.gene039185:intron 45.0%
CAAGAACAGGTAACTCGTTG+CGG - chr2.1:28285109-28285128 MS.gene039185:intron 45.0%
CAGCCTTCCAATTCTTGTGA+AGG - chr2.1:28285928-28285947 MS.gene039185:intron 45.0%
CATAATCTTGGTATGGCACG+AGG + chr2.1:28287184-28287203 None:intergenic 45.0%
CTCCCTTCACAAGAATTGGA+AGG + chr2.1:28285934-28285953 None:intergenic 45.0%
CTCCTGATTATTTCAGCCAG+AGG + chr2.1:28286825-28286844 None:intergenic 45.0%
CTTCACAAGAATTGGAAGGC+TGG + chr2.1:28285930-28285949 None:intergenic 45.0%
GACATGTCAACTACTGTCCT+TGG - chr2.1:28283529-28283548 MS.gene039185:intron 45.0%
GACCTCTGGCTGAAATAATC+AGG - chr2.1:28286820-28286839 MS.gene039185:CDS 45.0%
GAGTGATTTCCTTGAGTGAG+CGG + chr2.1:28287120-28287139 None:intergenic 45.0%
GCAGTTGGAGCAATCATGAT+TGG + chr2.1:28283571-28283590 None:intergenic 45.0%
GCGTTTGACTACTATGCATC+TGG - chr2.1:28283267-28283286 MS.gene039185:CDS 45.0%
GTGAGAAGAGCTGAAAAAGC+TGG - chr2.1:28285026-28285045 MS.gene039185:intron 45.0%
TAGGTTGTGAAAGCTGCTCA+AGG - chr2.1:28286396-28286415 MS.gene039185:intron 45.0%
TGCCAAAGCCTTGAAGACAT+CGG + chr2.1:28286463-28286482 None:intergenic 45.0%
TGGAGAAGCTGGTGTAAGAA+AGG - chr2.1:28287042-28287061 MS.gene039185:intron 45.0%
TGGAGCAATCATGATTGGCA+TGG + chr2.1:28283566-28283585 None:intergenic 45.0%
! AACTGGAAAAAGCGGATTCC+AGG + chr2.1:28284851-28284870 None:intergenic 45.0%
! GATAACGGCAGTACTAATGG+TGG + chr2.1:28286960-28286979 None:intergenic 45.0%
! GGACTTGCTTCATATGTTGC+TGG - chr2.1:28285681-28285700 MS.gene039185:intron 45.0%
! GTCTTGGAGTATCAACGGTA+AGG + chr2.1:28285067-28285086 None:intergenic 45.0%
! TCTAAGGCACTTATGGTTGC+TGG + chr2.1:28286272-28286291 None:intergenic 45.0%
! TGACCACATCGTTGCTGATT+GGG - chr2.1:28287141-28287160 MS.gene039185:CDS 45.0%
ATGGCACGAGGATTAACACG+AGG + chr2.1:28287172-28287191 None:intergenic 50.0%
CAGAAAATGGCTCATCCCGA+GGG - chr2.1:28283595-28283614 MS.gene039185:intron 50.0%
CAGACGCGCAATCAACACTT+TGG + chr2.1:28286712-28286731 None:intergenic 50.0%
CAGCCAGAGAATACACCACA+GGG + chr2.1:28287018-28287037 None:intergenic 50.0%
GTCGAAGAAGTTGCTTCCAC+TGG - chr2.1:28284824-28284843 MS.gene039185:intron 50.0%
GTGACCACATCGTTGCTGAT+TGG - chr2.1:28287140-28287159 MS.gene039185:CDS 50.0%
GTGTATTCTCTGGCTGCTGA+TGG - chr2.1:28287022-28287041 MS.gene039185:intron 50.0%
TAGTACCCTAGTTCGACCTC+TGG - chr2.1:28286806-28286825 MS.gene039185:CDS 50.0%
TCAGAAAATGGCTCATCCCG+AGG - chr2.1:28283594-28283613 MS.gene039185:intron 50.0%
TGATCGTACTCTGAGCTGGA+AGG - chr2.1:28285710-28285729 MS.gene039185:intron 50.0%
TGGCACGAGGATTAACACGA+GGG + chr2.1:28287171-28287190 None:intergenic 50.0%
TGTCCCAATCAGCAACGATG+TGG + chr2.1:28287147-28287166 None:intergenic 50.0%
TTCAGCCAGAGGTCGAACTA+GGG + chr2.1:28286814-28286833 None:intergenic 50.0%
TTTCAGCCAGAGGTCGAACT+AGG + chr2.1:28286815-28286834 None:intergenic 50.0%
! CTACTATGCATCTGGTGCTG+AGG - chr2.1:28283275-28283294 MS.gene039185:CDS 50.0%
! GTCTTCAAGGCTTTGGCACT+TGG - chr2.1:28286465-28286484 MS.gene039185:intron 50.0%
!! CCTTGAAGACATCGGTTCCA+CGG + chr2.1:28286455-28286474 None:intergenic 50.0%
AAGTGGATGCCGCTCACTCA+AGG - chr2.1:28287108-28287127 MS.gene039185:CDS 55.0%
ACGCCCTGTGGTGTATTCTC+TGG - chr2.1:28287012-28287031 MS.gene039185:intron 55.0%
CCGTGGAACCGATGTCTTCA+AGG - chr2.1:28286452-28286471 MS.gene039185:intron 55.0%
GAAGTTGCTTCCACTGGACC+TGG - chr2.1:28284830-28284849 MS.gene039185:CDS 55.0%
GCAAGGCTAGCAGTGCAAAG+TGG - chr2.1:28286194-28286213 MS.gene039185:intron 55.0%
GCAGCCAGAGAATACACCAC+AGG + chr2.1:28287019-28287038 None:intergenic 55.0%
GCTTCTCTGCGTCCAAGTCT+TGG + chr2.1:28285083-28285102 None:intergenic 55.0%
GGCACGAGGATTAACACGAG+GGG + chr2.1:28287170-28287189 None:intergenic 55.0%
TGTCTAGATTGGACGCCCTG+TGG - chr2.1:28287000-28287019 MS.gene039185:intron 55.0%
! AAAGCGGATTCCAGGTCCAG+TGG + chr2.1:28284843-28284862 None:intergenic 55.0%
!! GAAGGGAGTACTCACTGCAG+AGG - chr2.1:28285946-28285965 MS.gene039185:intron 55.0%
CTGGCTGCTGATGGAGAAGC+TGG - chr2.1:28287031-28287050 MS.gene039185:intron 60.0%
GCAAGAGCTGCATCAGCTGC+TGG - chr2.1:28284386-28284405 MS.gene039185:intron 60.0%
GCAGTGCAAAGTGGTGCAGC+TGG - chr2.1:28286203-28286222 MS.gene039185:intron 60.0%
! CATCTGGTGCTGAGGACCAG+TGG - chr2.1:28283283-28283302 MS.gene039185:CDS 60.0%
! TTGGATGGAGGTGTACGCCG+TGG - chr2.1:28286435-28286454 MS.gene039185:intron 60.0%
!! GCTTTGGCACTTGGTGCCTC+TGG - chr2.1:28286474-28286493 MS.gene039185:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 28283204 28287204 28283204 ID=MS.gene039185
chr2.1 mRNA 28283204 28287204 28283204 ID=MS.gene039185.t1;Parent=MS.gene039185
chr2.1 exon 28287065 28287204 28287065 ID=MS.gene039185.t1.exon1;Parent=MS.gene039185.t1
chr2.1 CDS 28287065 28287204 28287065 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28286792 28286916 28286792 ID=MS.gene039185.t1.exon2;Parent=MS.gene039185.t1
chr2.1 CDS 28286792 28286916 28286792 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28285990 28286036 28285990 ID=MS.gene039185.t1.exon3;Parent=MS.gene039185.t1
chr2.1 CDS 28285990 28286036 28285990 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28285534 28285611 28285534 ID=MS.gene039185.t1.exon4;Parent=MS.gene039185.t1
chr2.1 CDS 28285534 28285611 28285534 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28285291 28285415 28285291 ID=MS.gene039185.t1.exon5;Parent=MS.gene039185.t1
chr2.1 CDS 28285291 28285415 28285291 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28284829 28284895 28284829 ID=MS.gene039185.t1.exon6;Parent=MS.gene039185.t1
chr2.1 CDS 28284829 28284895 28284829 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28284677 28284739 28284677 ID=MS.gene039185.t1.exon7;Parent=MS.gene039185.t1
chr2.1 CDS 28284677 28284739 28284677 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28284437 28284506 28284437 ID=MS.gene039185.t1.exon8;Parent=MS.gene039185.t1
chr2.1 CDS 28284437 28284506 28284437 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28284113 28284213 28284113 ID=MS.gene039185.t1.exon9;Parent=MS.gene039185.t1
chr2.1 CDS 28284113 28284213 28284113 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28283905 28284009 28283905 ID=MS.gene039185.t1.exon10;Parent=MS.gene039185.t1
chr2.1 CDS 28283905 28284009 28283905 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
chr2.1 exon 28283204 28283401 28283204 ID=MS.gene039185.t1.exon11;Parent=MS.gene039185.t1
chr2.1 CDS 28283204 28283401 28283204 ID=cds.MS.gene039185.t1;Parent=MS.gene039185.t1
Gene Sequence

>MS.gene039185

ATGGGAGAGATAACCAATATCAGTGAGTATGAGGAAATTGCAAGGCAGAAATTGCCAAAGATGGCGTTTGACTACTATGCATCTGGTGCTGAGGACCAGTGGACTCTTCAAGAAAACAGAAATGCCTTTTCCAAAATTTTGTAAGATTTCATATAACTTATTTTGACAGTTCCTAAGTTACCAAATTATGTTACTTTATTATGTTCCTTTGATGTTTTGTACACCTACGACTCTGAGAACAAAAACAAATGTTAATGTGCTGAAAAATATGGTTGACAAACATTACAGGTTTAGGCCGCGAATTCTTATCGATGTGAGCAAGATAGACATGTCAACTACTGTCCTTGGCTTCAAAATTTCCATGCCAATCATGATTGCTCCAACTGCTTTTCAGAAAATGGCTCATCCCGAGGGTATAATTGCTTTTTCTTTAAATTCACTTGGATTTCTGAAATGGAAAATGAAGGTGTTTTGTTTTGTGCAATATTCTGTTCATTTTGCTAGAAGGGAAAAAAATCATGAAAAATCATGCATGACATGAATTGTGGAGAGTTTTAGCTTATATCACCCTTCTGGATACTTACATGCTATGAAAATGATTACTATAAGCATTTGTGAGACTATTTAGAAACAACTTATCTACTTGATTTATGTACAAAACGATAATTGAGACGGAGATCATTAAGTTTATCTGTGCTTATCAAAACAACTTATGATATGTTTATATGTTGTTTTTAGCTTATTTCCATAAGCTTCAAAATGTAGCTTATGAAAACAGCTTAGAGCTTAAATGAAAGTAATTTGACTTTATTTTATCTTTTGTTATAGAAATAACATATACACGAACACTGATACGATAAATGTTTATGCTTTACGCACTTACTCAAACTGTTTATCACAACTATGATAACTTGGTTTCTAGTTGTGTAACTTCCACTGATAAGCTATTACACTGAAATTAAGTGTGAATAAACATTAGTAATTCTTAAAAAAATATCATCTGCTAGTTTTGATAAATGGATAAAAACAAACTCAATGCTTTTTTATTAAGAAAACTCTACAATAGGATAGCAACATGAGATTTAAATTGTCGATGTTCTTGTATTATATATTATATTTCAAATCCCCTTTTGTGTTTAGTGACATGTACAATTTTTTTCTTGTGCAGGAGAATATGCAACAGCAAGAGCTGCATCAGCTGCTGGAACAATCATGGTACTTCTCTTCCTATTTTGTAATAAACATTTGATTTGTATACACTGTCATATAAAACATTTTTACACATGCGTTCAATCAAATCTTCCCATGTAGATGTTTGCAGAGATGTTTTAGAAACGAACAAAACTAAGTGACATGATAGTGTAATTTGATTGGATGCATGCGGAAACAATATTTGCATAGCACAATATTTGGAAAAAAAATGAAAATAGCTTATTACATGTCGGTAAAGTGTTTTTAGCATATGTAGAGTTTATCCTCATTACTTCTTCAATTAATTCTAAAATCAGAAAACACATAAGCTCTTATACGATTAACACTTATTTAACTGTATATTCAAATCAGTTACTTCAATTAACTTGTTGATTCTTTCAGACATTGTCATCATGGGCGACTTCAAGTGTCGAAGAAGTTGCTTCCACTGGACCTGGAATCCGCTTTTTCCAGTTATATGTGAGCAACAAAAACATTATTCTCTCAGTTCCATTTCTTCTTCACTTGAATTCTACGTGAAATAGTTCGTTTCTCTTATATTGTTATATGTTCTGTATTGGCCATGTTCTTTATTTAGGTGTACAAGGACAGGAATGTTGTTGCTCAGCTTGTGAGAAGAGCTGAAAAAGCTGGATTCAAGGCTATTGCCCTTACCGTTGATACTCCAAGACTTGGACGCAGAGAAGCTGATATCAAGAACAGGTAACTCGTTGCGGCTGTGTTGTGATTCTTAATATTGCAAAGAATCATATTTACATGAGGTTGATGCGGCCACAATTGCAATCACTATAACATAACTGAAATATCGCATTTATTGATAACTTGATCAATAAGGAATACAATAGACTCTATACATACGAGTTCATATGAAAAGGAAAAACATAACAAGGATAACTAACCTAACTAACTCTGTTAGATTAACAAATTCTTAACAAACTATATATCATCTAAAAGTCACGTTGCAATTGTGAAGACCTAAAAATTTTGATGTTGCAGGTTTATATTATTGTCGCAGACCTTTTTCAAAAACCAATATGCTTAGATTTTGAGTTTCAGCTTTAATGTAATAAAGATTAATTATAAATTTTCTTTTACAGATTTGTTTTGCCACCATTTTTGACATTGAAGAATTTTGAAGGACTGAACCTTGGAAAGATGGACGAAGTAAGTGAGATCAATTATTACAAAATTAGTAACATAGTTTAATGGTCTTACCTCTATAATTAATATAATTGTTACCTTGGTCCATTTAGGCTAATGACTCTGGACTTGCTTCATATGTTGCTGGTCAAATTGATCGTACTCTGAGCTGGAAGGTATGTCACCAATTTGTTCGCTCAATTCGTTGTTTTTGATTGATAAGTAGCATGCATGCTGACATTAGAAAAACAAAAGAAAACACTACTTTTGGTTGTCATTTTTAGTCTACATAATGGAATTTTCAAGGTTCCAATGATTTAGTGTTGTTTTTGTTAATTTTGTGCAGGATGTGAAGTGGCTTCAGACTATCACCAGCCTTCCAATTCTTGTGAAGGGAGTACTCACTGCAGAGGATAGTAAGCAAAACAAAACCTGTTTGAGAATTCTATTAATGCACTTTCTAACACATCTTTTTTGATACACTGTTATATTTAACACTTTTTTCAATACGCTAGTATTAGGGTCTCACATACATTATTTCATATAAAAGAAGTGATTACAACTAATTCACATAATTTAGTGAACTATAGATGAATTTTTTACGACTATTTTATTAATGGTTTGATGTAAATTTTGCAGCAAGGCTAGCAGTGCAAAGTGGTGCAGCTGGAATTATTGTGTCCAACCATGGAGCAAGACAACTTGATTATGTTCCAGCAACCATAAGTGCCTTAGAAGAGGTACTTTAAGAATGTCAAGTTATTATATAACTGAATTAATTGTATAAATCATTCTACATAATAAGATTGTTTATATCATAATTGTGTTGCTGAAATTACTTAGGTTGTGAAAGCTGCTCAAGGTCGTGTTCCTGTATTTTTGGATGGAGGTGTACGCCGTGGAACCGATGTCTTCAAGGCTTTGGCACTTGGTGCCTCTGGCATATTTGTGAGTATACTCATAGCATTTCCCAATATTTTAAATTCTCATTGCCTCATTAGTAGTAATTTCTTAATTCGGATCGTCTATGGTCACAATGTCGACGCATTCATGTAGTTAACGGTTTGGAGCTGTTGTGACTCTTATTTTAAGCTCTCTAAGTCAACCATAAAAGCCAACTTTATCAAATTCGTTTGATTTTGATCAAACATCTCCAAAGTGTTGATTGCGCGTCTGAATTCATAATTCTTGAGTGGCTATAGTATCTATGAACCCTGTTAATCTTAAACACAATGGTTGAAATATGCGAGTAGTACCCTAGTTCGACCTCTGGCTGAAATAATCAGGAGTAATCATTGATCAACTTAATTTACCTCCCGACCAAACTTTTATTACGACACTTGTCTTCTATAATATATGGAGGATTAAGACGAACAAAACCAAAATAATTTATCGTTATAACCACCATTAGTACTGCCGTTATCTTAACGAACAAATTATGCAATGTCTAGATTGGACGCCCTGTGGTGTATTCTCTGGCTGCTGATGGAGAAGCTGGTGTAAGAAAGGTCCTTCAAATGTTACGTGATGAGTTTGAGTTAACAATGGCTTTAAGTGGATGCCGCTCACTCAAGGAAATCACTCGTGACCACATCGTTGCTGATTGGGACACCCCTCGTGTTAATCCTCGTGCCATACCAAGATTATGA
Protein sequence

>MS.gene039185.t1

MGEITNISEYEEIARQKLPKMAFDYYASGAEDQWTLQENRNAFSKILFRPRILIDVSKIDMSTTVLGFKISMPIMIAPTAFQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGLNLGKMDEANDSGLASYVAGQIDRTLSWKDVKWLQTITSLPILVKGVLTAEDTRLAVQSGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVYSLAADGEAGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIVADWDTPRVNPRAIPRL