AlfalfaGEDB | MS.gene040117

M. sativa cultivar XinJiangDaYe / MS.gene040117

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00515.31 PF01425.24 PF07719.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040117.t1	XP_003631114.1	93.6	594	12	1	1	568	1	594	0.00E+00	1077.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040117.t1	Q9MUK5	77.3	595	107	2	1	569	1	593	2.0e-252	872.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040117.t1	G7LE58	93.6	594	12	1	1	568	1	594	0.0e+00	1077.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene90875	MS.gene040117	PPI
MS.gene017032	MS.gene040117	PPI
MS.gene019162	MS.gene040117	PPI
MS.gene029150	MS.gene040117	PPI
MS.gene93101	MS.gene040117	PPI
MS.gene47995	MS.gene040117	PPI
MS.gene46950	MS.gene040117	PPI
MS.gene037397	MS.gene040117	PPI
MS.gene22216	MS.gene040117	PPI
MS.gene040117	MS.gene47674	PPI
MS.gene038240	MS.gene040117	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040117.t1	MTR_8g107280	93.603	594	12	1	1	568	1	594	0.0	1139
MS.gene040117.t1	MTR_8g107280	92.979	527	11	1	1	501	1	527	0.0	1003
MS.gene040117.t1	MTR_4g094205	48.080	599	278	4	1	567	5	602	0.0	549
MS.gene040117.t1	MTR_1g082750	49.235	392	198	1	42	432	32	423	7.21e-141	414
MS.gene040117.t1	MTR_4g126750	80.882	68	13	0	500	567	80	147	1.61e-30	117
MS.gene040117.t1	MTR_1g020070	85.075	67	10	0	501	567	157	223	5.76e-30	117
MS.gene040117.t1	MTR_1g084100	34.868	152	95	3	406	555	43	192	7.39e-19	90.1
MS.gene040117.t1	MTR_3g452710	42.105	114	66	0	453	566	11	124	3.12e-18	88.2
MS.gene040117.t1	MTR_3g452710	42.105	114	66	0	453	566	11	124	3.65e-18	88.2
MS.gene040117.t1	MTR_2g010180	34.694	147	94	2	40	185	198	343	4.01e-16	82.0
MS.gene040117.t1	MTR_2g010180	34.694	147	94	2	40	185	198	343	4.33e-16	82.0
MS.gene040117.t1	MTR_2g010180	34.694	147	94	2	40	185	212	357	4.37e-16	82.0
MS.gene040117.t1	MTR_7g059150	33.553	152	85	3	431	567	66	216	4.90e-16	79.0
MS.gene040117.t1	MTR_8g094740	36.923	130	82	0	56	185	221	350	2.41e-15	79.3
MS.gene040117.t1	MTR_8g094740	36.923	130	82	0	56	185	221	350	2.86e-15	79.3
MS.gene040117.t1	MTR_3g096980	33.846	130	86	0	56	185	220	349	3.49e-15	79.0
MS.gene040117.t1	MTR_2g090135	34.426	122	73	1	453	567	98	219	1.97e-14	74.3
MS.gene040117.t1	MTR_1g030300	41.228	114	63	2	453	565	181	291	5.12e-14	75.1
MS.gene040117.t1	MTR_8g080890	36.607	112	65	2	76	182	144	254	3.69e-13	72.4
MS.gene040117.t1	MTR_8g080840	37.500	112	64	2	76	182	143	253	1.20e-12	70.9
MS.gene040117.t1	MTR_8g095600	36.697	109	64	2	79	186	266	370	3.74e-12	69.3
MS.gene040117.t1	MTR_8g080870	37.500	112	64	2	76	182	114	224	3.86e-12	68.9
MS.gene040117.t1	MTR_4g120360	36.885	122	71	2	452	567	344	465	7.27e-12	68.2
MS.gene040117.t1	MTR_8g095600	36.697	109	64	2	79	186	282	386	7.87e-12	68.6
MS.gene040117.t1	MTR_8g095600	36.697	109	64	2	79	186	266	370	8.06e-12	68.6
MS.gene040117.t1	MTR_8g095600	36.697	109	64	2	79	186	266	370	8.50e-12	68.2
MS.gene040117.t1	MTR_8g097220	35.714	112	66	2	76	182	140	250	9.18e-12	68.2
MS.gene040117.t1	MTR_3g048950	40.541	111	66	0	75	185	143	253	1.61e-11	67.4
MS.gene040117.t1	MTR_5g012030	29.630	135	89	1	421	555	366	494	2.40e-11	67.0
MS.gene040117.t1	MTR_4g074030	40.789	76	45	0	107	182	171	246	3.07e-11	66.2
MS.gene040117.t1	MTR_4g132780	33.051	118	73	2	457	568	43	160	6.37e-11	65.9
MS.gene040117.t1	MTR_8g011720	34.579	107	70	0	444	550	2	108	6.92e-11	63.5
MS.gene040117.t1	MTR_8g080880	35.714	112	66	2	76	182	143	253	9.27e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene040117.t1	AT3G17970	63.251	566	182	1	28	567	23	588	0.0	714
MS.gene040117.t1	AT3G17970	62.162	518	170	1	28	519	23	540	0.0	642
MS.gene040117.t1	AT5G09420	48.658	596	274	4	3	566	6	601	0.0	579
MS.gene040117.t1	AT1G08980	47.608	418	192	3	36	426	4	421	5.47e-133	394
MS.gene040117.t1	AT1G56440	51.042	96	46	1	457	552	78	172	2.69e-22	100
MS.gene040117.t1	AT1G56440	51.042	96	46	1	457	552	88	182	3.56e-22	100
MS.gene040117.t1	AT2G42810	36.290	124	79	0	443	566	3	126	1.05e-16	83.6
MS.gene040117.t1	AT2G42810	36.290	124	79	0	443	566	3	126	1.10e-16	83.6
MS.gene040117.t1	AT2G42810	36.290	124	79	0	443	566	3	126	1.34e-16	83.2
MS.gene040117.t1	AT2G42810	36.290	124	79	0	443	566	3	126	1.34e-16	83.2
MS.gene040117.t1	AT2G42810	36.290	124	79	0	443	566	3	126	1.34e-16	83.2
MS.gene040117.t1	AT5G64440	34.615	130	85	0	56	185	215	344	5.36e-16	81.6
MS.gene040117.t1	AT1G04190	39.640	111	67	0	457	567	19	129	2.99e-14	74.7
MS.gene040117.t1	AT4G08320	40.351	114	64	2	453	565	175	285	5.83e-14	74.7
MS.gene040117.t1	AT4G08320	40.351	114	64	2	453	565	175	285	7.44e-14	74.3
MS.gene040117.t1	AT3G25660	36.429	140	85	1	50	185	115	254	2.07e-12	70.1
MS.gene040117.t1	AT5G07360	36.697	109	64	2	79	186	308	412	4.65e-12	69.3
MS.gene040117.t1	AT5G07360	36.697	109	64	2	79	186	308	412	4.65e-12	69.3
MS.gene040117.t1	AT1G12270	33.645	107	71	0	457	563	387	493	1.98e-11	67.0
MS.gene040117.t1	AT1G62740	32.710	107	72	0	457	563	386	492	3.42e-11	66.2
MS.gene040117.t1	AT4G12400	39.806	103	62	0	453	555	2	104	4.10e-11	66.2
MS.gene040117.t1	AT4G12400	33.981	103	68	0	453	555	369	471	5.71e-11	65.9
MS.gene040117.t1	AT4G12400	39.806	103	62	0	453	555	2	104	4.92e-11	65.9
MS.gene040117.t1	AT4G12400	33.981	103	68	0	453	555	369	471	7.22e-11	65.5

Find 150 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAATCTTCGAACAATTTA+TGG	0.057750	4.4:-301605	MS.gene040117:CDS
ATCTCCTTTCCACCTAATTT+TGG	0.162409	4.4:+296953	None:intergenic
TCTATTTGCAGACATGAATT+TGG	0.170636	4.4:-298643	MS.gene040117:intron
CTGCCACTGCTTATCTTTGT+AGG	0.184219	4.4:+295701	None:intergenic
AGTGGCGAAAATAAGCATTT+TGG	0.234600	4.4:-301056	MS.gene040117:CDS
TCTTAAGGACGAAGGAATTT+TGG	0.240223	4.4:-297002	MS.gene040117:intron
AGCAATAAGTCTTGACAAAA+AGG	0.253299	4.4:-295480	MS.gene040117:intron
CTAAGTATTGCTAGCATATC+AGG	0.267474	4.4:-296893	MS.gene040117:CDS
GAACAATTTATGGGTATTAC+TGG	0.271346	4.4:-301595	MS.gene040117:CDS
TTGTAGAAATTGCATAATAT+TGG	0.276970	4.4:+298938	None:intergenic
GTTTCTTCTCTTGTTCAATC+TGG	0.285378	4.4:-301230	MS.gene040117:CDS
ACGCAACATACTACAGTAAC+AGG	0.292638	4.4:-295632	MS.gene040117:CDS
TTATGGGTATTACTGGGTTT+GGG	0.305409	4.4:-301588	MS.gene040117:CDS
GCTGATCCTCCTCCAAAATT+AGG	0.309333	4.4:-296965	MS.gene040117:CDS
AATTTAGTTGATTTCTCCTT+GGG	0.312635	4.4:-300957	MS.gene040117:intron
TTTATGGGTATTACTGGGTT+TGG	0.318931	4.4:-301589	MS.gene040117:CDS
CACCACCATAACGAGCTATC+AGG	0.326660	4.4:+296529	None:intergenic
GCTGGATTCTGTGGGATACT+TGG	0.328456	4.4:-299873	MS.gene040117:CDS
CCCAGCTTGAAGGCGTGTTC+TGG	0.331605	4.4:-299008	MS.gene040117:CDS
TTGCAGCAATACCTTCTCTT+TGG	0.333315	4.4:+296386	None:intergenic
TTCTCGACCACCTCTCTTAC+GGG	0.335821	4.4:-301184	MS.gene040117:intron
AGAGGACGCCATTGATTTCA+TGG	0.348851	4.4:+301626	None:intergenic
TCAATCTTCGAACAATTTAT+GGG	0.360486	4.4:-301604	MS.gene040117:CDS
CCAACTCTACGCAGTATATC+AGG	0.360852	4.4:+299390	None:intergenic
ATTACAGGCGTTGATACTTC+TGG	0.364126	4.4:-299912	MS.gene040117:intron
TCGTGCGTCCTTGCCCATTC+CGG	0.364934	4.4:+301278	None:intergenic
CGTGTCTACGTTTGGTCATC+CGG	0.366026	4.4:-301297	MS.gene040117:CDS
TCACATGGTGCTGTTTCTCA+TGG	0.367567	4.4:-299840	MS.gene040117:CDS
TAATTTAGTTGATTTCTCCT+TGG	0.368566	4.4:-300958	MS.gene040117:CDS
CATCGGCAATAATTATTTGC+CGG	0.373526	4.4:+299325	None:intergenic
GTTCTCGACCACCTCTCTTA+CGG	0.377000	4.4:-301185	MS.gene040117:CDS
ACAAACAGATTTGACATAGA+AGG	0.383943	4.4:-301323	MS.gene040117:intron
TCCTGTCAAGAGGAACATTT+AGG	0.392168	4.4:+299289	None:intergenic
GAAACAGCTGGGTGAAGATC+AGG	0.407748	4.4:+298586	None:intergenic
CAAACTGTGAAAGAGGATTT+CGG	0.423798	4.4:-301528	MS.gene040117:CDS
CACATGGTGCTGTTTCTCAT+GGG	0.425188	4.4:-299839	MS.gene040117:CDS
AGGTACAGCTAGAGAGATGC+TGG	0.426080	4.4:-295006	MS.gene040117:CDS
ATCTAAAGTTCCCAGCTTGA+AGG	0.426528	4.4:-299018	MS.gene040117:CDS
ATTTGATTCCATGAAATCAA+TGG	0.431972	4.4:-301634	None:intergenic
GCCGCAGAGTTTAATAGCTT+CGG	0.438460	4.4:+295659	None:intergenic
GTTTCCCTGATAGCTCGTTA+TGG	0.438795	4.4:-296534	MS.gene040117:CDS
TACTTGAGCCAACCAACAAA+AGG	0.440758	4.4:-294500	MS.gene040117:CDS
CGGGGATAGCGGGATTGGTA+GGG	0.444809	4.4:+301030	None:intergenic
ATGCACGGCTCTGATAATCC+TGG	0.446102	4.4:+296924	None:intergenic
TGTTGGTTGGCTCAAGTACA+AGG	0.448590	4.4:+294505	None:intergenic
ATCGGCAATAATTATTTGCC+GGG	0.453217	4.4:+299326	None:intergenic
CATACCCGTTTCAACAAGTC+TGG	0.453788	4.4:-299811	MS.gene040117:CDS
AGGGTGCTTGTAAAAGTATA+TGG	0.454570	4.4:+299367	None:intergenic
GCCAGAACACGCCTTCAAGC+TGG	0.458558	4.4:+299007	None:intergenic
TACGTTTGGTCATCCGGAAT+GGG	0.460111	4.4:-301291	MS.gene040117:CDS
GGGGATAGCGGGATTGGTAG+GGG	0.463096	4.4:+301031	None:intergenic
GGAGGGGTGAGAGTGCCTGC+TGG	0.464288	4.4:-299891	MS.gene040117:CDS
GGTAGACGAGCGGGGATAGC+GGG	0.466037	4.4:+301020	None:intergenic
GCGGGGATAGCGGGATTGGT+AGG	0.471210	4.4:+301029	None:intergenic
GCTATCCCCGCTCGTCTACC+TGG	0.473289	4.4:-301017	MS.gene040117:CDS
TTACTGGGTTTGGGTTTAGC+AGG	0.473413	4.4:-301579	MS.gene040117:CDS
CTACGTTTGGTCATCCGGAA+TGG	0.475998	4.4:-301292	MS.gene040117:CDS
ACCTAAATGTTCCTCTTGAC+AGG	0.479448	4.4:-299290	MS.gene040117:CDS
GCTTAAACAAACTGTGAAAG+AGG	0.483235	4.4:-301535	MS.gene040117:CDS
AGAGTGCCTGCTGGATTCTG+TGG	0.483495	4.4:-299882	MS.gene040117:CDS
GAGCCGCCAGGTAGACGAGC+GGG	0.484166	4.4:+301011	None:intergenic
TTTCAGGTCACAGTACCATT+GGG	0.485220	4.4:-296582	MS.gene040117:intron
GAAGGACACGTGTCTACGTT+TGG	0.487354	4.4:-301305	MS.gene040117:CDS
GCTATCAGGGAAACTGAAAC+AGG	0.492446	4.4:+296543	None:intergenic
TACTACAAAGAAGCAATTGA+TGG	0.495869	4.4:-294981	MS.gene040117:intron
CTCTTGTAGACTTGTATACA+TGG	0.501692	4.4:+296479	None:intergenic
GTGTCCAGACTTGTTGAAAC+GGG	0.502655	4.4:+299807	None:intergenic
TGCAATTGTGCTGAAACAGC+TGG	0.503072	4.4:+298574	None:intergenic
GCACCACTAGAGGAGCCGCC+AGG	0.504143	4.4:+300999	None:intergenic
ACATGGTGCTGTTTCTCATG+GGG	0.504209	4.4:-299838	MS.gene040117:CDS
GCTCAAGCGTATCTTGAACT+TGG	0.504250	4.4:-295609	MS.gene040117:CDS
ATATGCTAGCAATACTTAGC+AGG	0.504439	4.4:+296897	None:intergenic
CTTCAGCTGCTGAAAGATTG+AGG	0.506401	4.4:-294473	MS.gene040117:CDS
TCAACAAGTCTGGACACTCT+TGG	0.508893	4.4:-299801	MS.gene040117:intron
GAGAACGGTAGTAGCAATAC+AGG	0.511457	4.4:+301202	None:intergenic
ACGAGCGGGGATAGCGGGAT+TGG	0.514474	4.4:+301025	None:intergenic
AAGCAGTGGCAGAAAGCCAT+TGG	0.520099	4.4:-295690	MS.gene040117:CDS
CTACCTGGCGGCTCCTCTAG+TGG	0.521414	4.4:-301002	MS.gene040117:CDS
TCCTCCAAAATTAGGTGGAA+AGG	0.522866	4.4:-296957	MS.gene040117:CDS
TGGAAAGGAGATCCTATCCC+AGG	0.526894	4.4:-296942	MS.gene040117:CDS
GTAAGAGAGGTGGTCGAGAA+CGG	0.527108	4.4:+301187	None:intergenic
ATCAAGAAATGTTGTTAGCA+AGG	0.529714	4.4:-296427	MS.gene040117:CDS
GGAGGAGGATCAGCTACAGT+AGG	0.529844	4.4:+296974	None:intergenic
TTGTAATGCAACGCAGTCCC+CGG	0.534546	4.4:-299344	MS.gene040117:CDS
CCAGAACACGCCTTCAAGCT+GGG	0.534601	4.4:+299008	None:intergenic
AGTGTCCAGACTTGTTGAAA+CGG	0.534842	4.4:+299806	None:intergenic
TAGCATATCAGGTTGCTGTC+AGG	0.535420	4.4:-296882	MS.gene040117:intron
ATCAACTAAATTAGCAGCAA+CGG	0.535637	4.4:+300968	None:intergenic
TGATGTATCCGAAGTGGAGA+TGG	0.539165	4.4:-298542	MS.gene040117:CDS
CGCTGAGATTGCCAAAGAGA+AGG	0.549950	4.4:-296397	MS.gene040117:intron
TGCACGGCTCTGATAATCCT+GGG	0.556763	4.4:+296925	None:intergenic
ATATTATGCAATTTCTACAA+AGG	0.559204	4.4:-298935	MS.gene040117:intron
CTTTATTAATTCAGCATCAG+TGG	0.560639	4.4:-301074	MS.gene040117:intron
AACACGCATCTCACTTCTAA+CGG	0.561508	4.4:+298507	None:intergenic
GTATACAAGTCTACAAGAGC+AGG	0.561649	4.4:-296475	MS.gene040117:CDS
TGAACAAGAGAAGAAACTGC+AGG	0.562264	4.4:+301236	None:intergenic
GCAGGGGAAGTAGAAGAAGC+AGG	0.570121	4.4:+301254	None:intergenic
TTGGTCATCCGGAATGGGCA+AGG	0.571161	4.4:-301286	MS.gene040117:CDS
AACAATTTATGGGTATTACT+GGG	0.571256	4.4:-301594	MS.gene040117:CDS
CCTGATATACTGCGTAGAGT+TGG	0.571803	4.4:-299390	MS.gene040117:CDS
TCTCTCTAGCTGTACCTCTA+CGG	0.572236	4.4:+295012	None:intergenic
TTGTACAATCTACCTCAGCT+TGG	0.572790	4.4:+295504	None:intergenic
CTTGGATTTCGACCTTCACA+TGG	0.573818	4.4:-299855	MS.gene040117:CDS
ACTTGAGCCAACCAACAAAA+GGG	0.575182	4.4:-294499	MS.gene040117:CDS
TACTTAGCAGGCTAAATGCA+CGG	0.575999	4.4:+296909	None:intergenic
AAGCGTATCTTGAACTTGGA+AGG	0.576073	4.4:-295605	MS.gene040117:intron
AAAATTTGATGTATCCGAAG+TGG	0.576094	4.4:-298548	MS.gene040117:CDS
AGTATCCCACAGAATCCAGC+AGG	0.584224	4.4:+299876	None:intergenic
GGGACTGCGTTGCATTACAA+AGG	0.587842	4.4:+299347	None:intergenic
TAAATTGTTCGAAGATTGAG+AGG	0.588658	4.4:+301608	None:intergenic
GATCCTCCTCCAAAATTAGG+TGG	0.588943	4.4:-296962	MS.gene040117:CDS
ACTTGAGAACTCCTGTCAAG+AGG	0.590616	4.4:+299279	None:intergenic
GGACTGCGTTGCATTACAAA+GGG	0.592007	4.4:+299348	None:intergenic
AGCTGTACCTCTACGGAAGT+AGG	0.593410	4.4:+295019	None:intergenic
ACCACCATAACGAGCTATCA+GGG	0.599413	4.4:+296530	None:intergenic
GGCAACGGCAGCACCACTAG+AGG	0.600360	4.4:+300989	None:intergenic
TAGGTGTTGAAAACAGTCAT+CGG	0.600416	4.4:+299308	None:intergenic
GAACAAGAGAAGAAACTGCA+GGG	0.604221	4.4:+301237	None:intergenic
GAGTGCCTGCTGGATTCTGT+GGG	0.604435	4.4:-299881	MS.gene040117:CDS
GGCTCTGATAATCCTGGGAT+AGG	0.606125	4.4:+296930	None:intergenic
GGTACAGCTAGAGAGATGCT+GGG	0.607880	4.4:-295005	MS.gene040117:CDS
CATGGTGCTGTTTCTCATGG+GGG	0.611160	4.4:-299837	MS.gene040117:CDS
GAGGAGGATCAGCTACAGTA+GGG	0.611389	4.4:+296975	None:intergenic
TCCCTGATAGCTCGTTATGG+TGG	0.617870	4.4:-296531	MS.gene040117:CDS
AACGAACGAACCCGTAAGAG+AGG	0.619370	4.4:+301174	None:intergenic
TCTTGACAGGAGTTCTCAAG+TGG	0.620973	4.4:-299277	MS.gene040117:CDS
CTTAATGCTCTTAAGGACGA+AGG	0.626087	4.4:-297010	MS.gene040117:intron
AGCTTCGGTATAAAATCCAA+TGG	0.627667	4.4:+295674	None:intergenic
TTGGACATGTGCATGATGAG+TGG	0.628329	4.4:-298624	MS.gene040117:CDS
ACAGGCGTTGATACTTCTGG+AGG	0.628596	4.4:-299909	MS.gene040117:intron
CAGGCGTTGATACTTCTGGA+GGG	0.630046	4.4:-299908	MS.gene040117:CDS
TGAGAAACAGCACCATGTGA+AGG	0.630301	4.4:+299843	None:intergenic
GTTGGTTGGCTCAAGTACAA+GGG	0.632408	4.4:+294506	None:intergenic
CTTCTCTTTGGCAATCTCAG+CGG	0.638681	4.4:+296398	None:intergenic
CGCAACATACTACAGTAACA+GGG	0.640100	4.4:-295631	MS.gene040117:CDS
TAAATTAGCAGCAACGGCAA+CGG	0.640487	4.4:+300974	None:intergenic
AGCCGCCAGGTAGACGAGCG+GGG	0.644394	4.4:+301012	None:intergenic
AGGTAGACGAGCGGGGATAG+CGG	0.644820	4.4:+301019	None:intergenic
GAACGAACCCGTAAGAGAGG+TGG	0.650427	4.4:+301177	None:intergenic
TACTTGTCATAAAATCCCAA+TGG	0.651161	4.4:+296567	None:intergenic
AAGCCTACAAAGATAAGCAG+TGG	0.651878	4.4:-295704	MS.gene040117:CDS
GCAATTGTGCTGAAACAGCT+GGG	0.652367	4.4:+298575	None:intergenic
AGGCGTTGATACTTCTGGAG+GGG	0.657859	4.4:-299907	MS.gene040117:CDS
ATCCAGTTATCTCCAAGCTG+AGG	0.660098	4.4:-295516	MS.gene040117:intron
TCGGCAATAATTATTTGCCG+GGG	0.663537	4.4:+299327	None:intergenic
GTGAAGGCCTACTTCCGTAG+AGG	0.686734	4.4:-295026	MS.gene040117:CDS
GGAGCCGCCAGGTAGACGAG+CGG	0.687249	4.4:+301010	None:intergenic
AACAAGAGAAGAAACTGCAG+GGG	0.691862	4.4:+301238	None:intergenic
ATCCCCGCTCGTCTACCTGG+CGG	0.715764	4.4:-301014	MS.gene040117:CDS
ACCGAAGCTATTAAACTCTG+CGG	0.721325	4.4:-295660	MS.gene040117:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	294458	301646	294458	ID=MS.gene040117
chr4.4	mRNA	294458	301646	294458	ID=MS.gene040117.t1;Parent=MS.gene040117
chr4.4	exon	301507	301646	301507	ID=MS.gene040117.t1.exon1;Parent=MS.gene040117.t1
chr4.4	CDS	301507	301646	301507	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	301185	301337	301185	ID=MS.gene040117.t1.exon2;Parent=MS.gene040117.t1
chr4.4	CDS	301185	301337	301185	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	300958	301082	300958	ID=MS.gene040117.t1.exon3;Parent=MS.gene040117.t1
chr4.4	CDS	300958	301082	300958	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	299802	299927	299802	ID=MS.gene040117.t1.exon4;Parent=MS.gene040117.t1
chr4.4	CDS	299802	299927	299802	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	299247	299429	299247	ID=MS.gene040117.t1.exon5;Parent=MS.gene040117.t1
chr4.4	CDS	299247	299429	299247	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	298936	299080	298936	ID=MS.gene040117.t1.exon6;Parent=MS.gene040117.t1
chr4.4	CDS	298936	299080	298936	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	298486	298654	298486	ID=MS.gene040117.t1.exon7;Parent=MS.gene040117.t1
chr4.4	CDS	298486	298654	298486	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	296883	297017	296883	ID=MS.gene040117.t1.exon8;Parent=MS.gene040117.t1
chr4.4	CDS	296883	297017	296883	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	296398	296598	296398	ID=MS.gene040117.t1.exon9;Parent=MS.gene040117.t1
chr4.4	CDS	296398	296598	296398	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	295606	295733	295606	ID=MS.gene040117.t1.exon10;Parent=MS.gene040117.t1
chr4.4	CDS	295606	295733	295606	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	295481	295532	295481	ID=MS.gene040117.t1.exon11;Parent=MS.gene040117.t1
chr4.4	CDS	295481	295532	295481	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	294982	295051	294982	ID=MS.gene040117.t1.exon12;Parent=MS.gene040117.t1
chr4.4	CDS	294982	295051	294982	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1
chr4.4	exon	294458	294540	294458	ID=MS.gene040117.t1.exon13;Parent=MS.gene040117.t1
chr4.4	CDS	294458	294540	294458	ID=cds.MS.gene040117.t1;Parent=MS.gene040117.t1

Gene Sequence

>MS.gene040117

ATGAAATCAATGGCGTCCTCTCAATCTTCGAACAATTTATGGGTATTACTGGGTTTGGGTTTAGCAGGAATTTATGTTTTAACGAGAAAGCTTAAACAAACTGTGAAAGAGGATTTCGGTGCTTTCATTCAAAAGCTTCAGTTGCTTCCTCCTCCGCCTCCTGCTCCTCCCAAAGCTCCTCATCCACTCACTTCTCTCACTTTCGCCATCTCCGACTTGTATGTCTTTTCTTTTCATTTCGTTTCGTTTCGTTTCTAATAACCTAATAACAGTTGATTGAATTGAATTGATTTGAATTTGAACAAACAGATTTGACATAGAAGGACACGTGTCTACGTTTGGTCATCCGGAATGGGCAAGGACGCACGAACCTGCTTCTTCTACTTCCCCTGCAGTTTCTTCTCTTGTTCAATCTGGTGCTACCTGTATTGCTACTACCGTTCTCGACCACCTCTCTTACGGGTTCGTTCGTTCCTCCAATAAACCCTACCTACCCTTTTTTACTTCATCATATAATTATCGTTCGTAATCGTAATAATAATTGTTACTTCTTTATTAATTCAGCATCAGTGGCGAAAATAAGCATTTTGGAACCCCTACCAATCCCGCTATCCCCGCTCGTCTACCTGGCGGCTCCTCTAGTGGTGCTGCCGTTGCCGTTGCTGCTAATTTAGTTGATTTCTCCTTGGGTCAGCTTCTTCTTATATCTTTTTACTACTCATTTATTAACATGCTTCAAACATGTTTGTTTAGTAGCCAGTCAATTATGGTTTCTAGTAAGCATGTTCTAAACTAACCTATTACTTGTTCAATGGATGCCAACTAAACTTCCGATGATAGCTATCAAAATTTATTATTAGTAATTTGAAGCTACCATACACTGCATTTGACCAGTGAAAGATCTTCTTTTCATAACCAGCCATCAAGTATCTTGACTCCATACTACAAAATGATAGGGAGATGGGATGTGTAAACTATGGGGTGATTCAACCGGTTCAGATTGGGAATGAAAAGAATGTATTATTATTATTATTATTATTATTGAATGAACAGCAACACCCAAACTCATGGATTTTCAAAACAAAATCCAGACTTCCCAATTGCCTAAAAGGCTCCAACTCTAATCAATCGACAAGATATATTATTCCCTAAGGCTTTGTTTGGGAGTTTGGAGGGAAGGGGAAGGGAAGGAAGGGCTTTGAGGGGTGAGAAATATAGGGGAAAATTGAGAAATCTTTCACATTTTTTTTAAGAGTATTTTTTTAGAGTGTGATAAATTAATACTTATGATTAATATATTTTTATTTTTAAGTATATTATAACAACATAGATCAAATTTGAATAATTCATATAAACCCTCTCAAACCCCCCAAAACCCTCCTCTGATACAATGTTTTAGTTCCCCCCAAATGAGGAGGGTTTTATAATTTGAAGAAAAATTAACCCCCCAATGTCCTCTATTTCTTTCACTTACTCCTTCCTTTTTTCCCAAGCCCTCCCCTCCCTTCCTCTCCAAACTCGCAAAACAAAGCCTAAGGCTCACCTCTTAATAAATTTTAAGGAAAGATGCTTCTGTAACTTTGAATGAATTCATTCTTGTTGCCACATAATGCGTACTTATATATGTCTGCGTAATATATTTATAGTTTTCTGGTTCAATATCACTAACATGTTGACATTTGAATGTATCTCATACGATTGCACCATTGTGTATTACAGGCGTTGATACTTCTGGAGGGGTGAGAGTGCCTGCTGGATTCTGTGGGATACTTGGATTTCGACCTTCACATGGTGCTGTTTCTCATGGGGGAATCATACCCGTTTCAACAAGTCTGGACACTCTTGGTATGATTGATTCGTTTATTTATTCTGATTTAAAATATGGGCTTCTGTGCTTAAGTGGCTGCTTGATTTATTTATTTTGAACGATTAGTATATTTGCATCGTATAAAGATCAAGTGCAAATTTGGATTGATTACTTTTTAGCAGCTTTTTTAGATGCTTTCTTCGACAATATTTTGATTATAAACAGTTGAACACAATGGGTAAGCTAATACACACCGAGAACAGGATAAAAAGATGTTCTATTACAGCCGTTTTATTTAAACTTTCTTCTTATAGTTGCTGGCACTTTCTTAGCTTTTATATGCAGATGCTATGCTTCTGCCATCTGCTTCAGTTTTGAAACTCATTTTACCTCTGAAATGCTCTTTTCAGGTTGGTTTGCAAAGGATCCTGATATACTGCGTAGAGTTGGCCATATACTTTTACAAGCACCCTTTGTAATGCAACGCAGTCCCCGGCAAATAATTATTGCCGATGACTGTTTTCAACACCTAAATGTTCCTCTTGACAGGAGTTCTCAAGTGGTGATCAAAGCCACCGAGAAGCTTTTTGGAAGTATGTTCTTTTGTTGTGTCCTTTGCAGTTTAATATAATCCCTATCGTTTTGGTTATCATCGAAACCTCCTGCCTCAGTTTGTGTGCTGGCTTGTGATTTTTGAATAACCTGTGCTTGTTCTTACTATCGCATTCTGTTATTTTTTTCATTATCAATTATCTTCAGAGCAAGTATTGAAGCATATAAATCTTGAAGACTATATAAGATCTAAAGTTCCCAGCTTGAAGGCGTGTTCTGGCCAAAAAGCAAATGGTGAACTGAAATCTTCTTCGTTGATATTGCTTGCCAATATTATGCAATTTCTACAAAGGTTAGTTGGAAATATCATTATAATTTGCAGTTTTACGGGAAAATTTGAATGGAAGTTTTTTATTTTATTTTTAAACTTGTGGTTTGGTAGAGTTGTTTTACATTTAGCAGTTTTGTTGTGTTTTGATCTATCCACTCTGTATCATGTCAAAACTCATAACTTCTCCTTTTCAATTAGTATTCCTCATTGTGGGTTTACTTAATTTATACTTGTTGTGTATTATGACCGTTTGCGGAAGCCTTGAATAATAATGTGATATTTTTTTGCCACTCTATTTGCAGACATGAATTTGGACATGTGCATGATGAGTGGATGAGCATAGTAAAGCCTGATCTTCACCCAGCTGTTTCAGCACAATTGCATGAAAAATTTGATGTATCCGAAGTGGAGATGGAAAACTCTAAATCCGTTAGAAGTGAGATGCGTGTTGCTGTAAATTCACTTTTGAAGGTAATCATTTTTTTTACTCTTCCCCTATATATCTTTGGGCTGTGTTTGGTAACACGAATAAGCTAGCTTATAAGCTTGTTTTGATATACTAAGATGTGTTTGGTAAAAAGCTTTTTCCACTAGCTTATAGCGTTTTTTGAGAAGCTATTTCAAGTAGCTTTTGAGCTTATAGCTGGTAGCTTTTTATATTTTCTTCCAATTTTAACCCTATTAATTTAATTATTTTTTAATAAAACATGTTCATGGCTTAAAAAATAACTGCCACGTTCATAGCTTATTTTTAATAAAACAACTGCCACATTTTTTCCTTTGAAAAAAAACTGCCATGCTTTATTTTATTTTTTAAGGAACGCTTTATATATATATATATATATATATATATATATATCTTAAAAGAAATGTTATATATTCTTGCTTACATTTTTTTCCACCTTCCTCTTATTTTTGGGATTCATAAAAGTAGAGATCAATGAGCTTTTGCCCTCTTTTGTATAAAAAAAAAAAAGCAGAGATCAAAATTGTCGGTGAAATTTTTTTGGAGACTAAAACCAAAAGTTTGAATATTTATAGGGATCCAAAACATAACTAACCATAAATCAAAATATTAAAAAATACATATGATACATTAGAAAAAATGTGTGTGTGTGTGTGTGTGTGTGTGTATATATATATATATATATATATATTATAACTTAAAAAAAATGTCCTTTTATGTCATTTTATACTTATCAGCCACTTCAACAGCTAATTTTAATAAACCAACTTTTCAGCTATAAGCTATCGGTTATAAGACATCAGCTATATGCTAGCTTTTCAGCTATCAGCTAGCTTATAGCTTATTTTTGCCAAACACACAGATCCTTTTGATTTGAAATCAATATTCATGCTGCCTCATTCCAATGTTAGCTACATTTTTTAAAGCGTTTATTTTACCAGTGGTTTATCCTTCTTGCTTTCTTAAACAAGCAGAAGAGTACATTCACAGATCATAGTGGGTGGATTTTAATGTGATTGTTAAAGAGTTAAAAGCTGTACTGAATTTTAACAAACTTATGGTTTTGGTGAATGGAACATGGTTAATTGCTTTTGTATGGAGTACCTTTAATTTGTATTTCCAACTCTATAAAAATTAACCAATATATTCACAGACAAGCAAAGTGTGTTGTGGGTGGATTATAATATCTGGTTGTTAAGGGTTAAAATTTATGCTGAATTTTACAAATTTATGGTGAACGTAACATTGATATTGAATGGGCAAAACATTTCTAAATAAGTTACTGAGAATATACTGCAGAACACATGCAGAAAATTCTTTCAAAGATGAAAGTGGATATTTTCAATCAATTTATGGTTTTGAATATACCAATTTTTATAAGTTTTCAATTTAGTATTTTAGTAATTTATATTCTGATAAGGGAAACATAGTTGTTCAGAATTTAATACATTTTAATGGCTTAATGCTCTTAAGGACGAAGGAATTTTGGTGATCCCTACTGTAGCTGATCCTCCTCCAAAATTAGGTGGAAAGGAGATCCTATCCCAGGATTATCAGAGCCGTGCATTTAGCCTGCTAAGTATTGCTAGCATATCAGGTTGCTGTCAGGTAAAGATTTCACCCTCATGAAACTTGCTTAATGTTATCCGTTTTTTTTTTGTTTTGTTTATATTTGTGACTGTTTTGTCTGTATTTTCAGAATTTGTTTATATTTCACGGAAACTTGTTTCCTCAATCAATATCATTGCACTCTTTGTTTGATTTTCATATGTGATAGAATTCAAATTACCAATATGCACCTGATGGGTATTTTATGAAATATGTGATAGGCACTCTTTCATAATTGTGATTGTAATGACTTTTGTTCATATTCTCCTAATTTATCTTTTCAGGTCACAGTACCATTGGGATTTTATGACAAGTATCCTGTTTCAGTTTCCCTGATAGCTCGTTATGGTGGTGATCGCTTTTTGCTTGACACGCTAAAGACCATGTATACAAGTCTACAAGAGCAGGCTGATATTGCTGCCACAAGTAAAACATCAAGAAATGTTGTTAGCAAGGAACAATCCGCTGAGATTGCCAAAGAGAAGGTATTGCTGCAAATGAATCAGATTCAGCAGTCACCTATTTTTATTTTTATTTTGTAATTGTTCTGTGTAACTGGCATGGTTATAGTTCTAAATCACCAGTGACGTTTAAAACTATAATATTATAAAACCATCTACGACAACAACAACCAAGCCTTTAGATCTTTCTTAATAGGTTCCCTAATAGTTTTTCTAGGTCTTCCTCTGCCTCGAGTGATTTGATGACCCTCCATCTGATCTACTCTCCTTACTACATAATCCATAGGTCTTCGTTGTAGATGCCCAAACCACCACCATCTTTTCTACGATAGGTGCTGCCCCAATCCTTTCTCTGATGTTGTCATTTCTAATCGTTTACATCTCCTATTTCTCCACCGTTTTGTACTATGGACCCAGGGTATTTAAATCGTGTAACTTGTGGTATGACATGATCTCCAACTTTACATCAGGGATAACATTACATTTGGTGTCTTTCATAACTTTCTCTATGCAAATGTAGGGATGATGCTTTCTTTAGGTGTTGTAATTTATCCTTGACCTTTCCCCTATAATTTAAGCTGCCCCTTTTATTAAGATGCATCTGATAGTTTTACTCGTTAATTCTCAGTTCCTTTTGTGTTTGTTCTCCTCACTCATTATTTTTGGGGTTCATCGTTCAATGATTCAGGGAAATCAAGCCTACAAAGATAAGCAGTGGCAGAAAGCCATTGGATTTTATACCGAAGCTATTAAACTCTGCGGCGATAACGCAACATACTACAGTAACAGGGCTCAAGCGTATCTTGAACTTGGAAGGTAGTGTTTCATTCTTTTGCTATTTCTCTCTGTGTTTCCTGCAAACTAATGGATTTGTTCTTTGTTAATCCAGTTATCTCCAAGCTGAGGTAGATTGTACAAAAGCAATAAGTCTTGACAAAAAGGTAACTTTCATCTTCGAAGAACTTTATCCTTTGAAGTTAATGTCATTGGTTTGAATGATGGCTGTCATTTTAGACTATGACAAAATCTTGTCATCATTTCAAATATACATGTTTGTTGAAAATGAAGACTTCATAATAATTGAATTGCTATTCAGTTTGTTAAGAACACATGCATTTTGTGTTGTATTGGATCCAACAATTTTTGCCCCTAGATTAATATAATTTCTATACGACACTCAGAATAAAACAAAGAGAAAAGGAGAAAGAAATAGATTTATGGAAAAAATATTTCCTGCTATTGTTCATTCTTTACTCTTCATACAAAGTCCAATTAAATAGTTCATATACGTGATTATTGTATGTATTTTTGAATGAGATAGCCATGGGCATTTTTCAGTAACGTCCTCTCTTTATTCTTGTTTTAAACAGAGTGTGAAGGCCTACTTCCGTAGAGGTACAGCTAGAGAGATGCTGGGCTACTACAAAGAAGCAATTGATGGTAAGATCTTATATTCTCAAATTTGTTTACATTTTCTCATTTCCTCTAGAAAATTGAAGAGGTTCTCAAACTCGAGAGCCAATACGAAGCTGTTAATAGCTAACATGATTCGCCACACATTTCCTTTTTGTTGAAGTTCATTTGGTTCTAAAATATATAAGATTTCACTTGGCTCTTGCTTATTGGGCTTAAGTTGATCCATTGCGCATATTTAGCTGATTTCCGTTTGTGGGCTTCTAGTATTTTTTCTTTTAGTTGGTTATGGGAACTCAAAAATCATGTTTTGATGATTATTAGAGGACTGTTCAAAATTCAAATGTTTGAACAAGACAACTGCATATTGACGGGGTTTACATTCTGTTATCCTCATCAGTTATCTCATTTTCATATGAACATTTTAGAATTTCAAAAGCATACACTAATAAAAGATTTGGCATACAGATTTTAAATATGCCCTTGTACTTGAGCCAACCAACAAAAGGGCTGCTTCAGCTGCTGAAAGATTGAGGAAACTATTTCAGTAG

Protein sequence

>MS.gene040117.t1

MKSMASSQSSNNLWVLLGLGLAGIYVLTRKLKQTVKEDFGAFIQKLQFDIEGHVSTFGHPEWARTHEPASSTSPAVSSLVQSGATCIATTVLDHLSYGISGENKHFGTPTNPAIPARLPGGSSSGAAVAVAANLVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHGGIIPVSTSLDTLGWFAKDPDILRRVGHILLQAPFVMQRSPRQIIIADDCFQHLNVPLDRSSQVVIKATEKLFGKQVLKHINLEDYIRSKVPSLKACSGQKANGELKSSSLILLANIMQFLQRHEFGHVHDEWMSIVKPDLHPAVSAQLHEKFDVSEVEMENSKSVRSEMRVAVNSLLKDEGILVIPTVADPPPKLGGKEILSQDYQSRAFSLLSIASISGCCQVTVPLGFYDKYPVSVSLIARYGGDRFLLDTLKTMYTSLQEQADIAATSKTSRNVVSKEQSAEIAKEKGNQAYKDKQWQKAIGFYTEAIKLCGDNATYYSNRAQAYLELGSYLQAEVDCTKAISLDKKSVKAYFRRGTAREMLGYYKEAIDDFKYALVLEPTNKRAASAAERLRKLFQ