Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019162.t1 | XP_013459622.1 | 97.8 | 581 | 13 | 0 | 1 | 581 | 1 | 581 | 0.00E+00 | 1144 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019162.t1 | Q9FNN2 | 58.4 | 589 | 231 | 4 | 1 | 577 | 1 | 587 | 1.4e-203 | 710.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019162.t1 | A0A072UX44 | 97.8 | 581 | 13 | 0 | 1 | 581 | 1 | 581 | 0.0e+00 | 1144.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene019162 | MS.gene82134 | PPI |
| MS.gene019162 | MS.gene059032 | PPI |
| MS.gene019162 | MS.gene029150 | PPI |
| MS.gene019162 | MS.gene90875 | PPI |
| MS.gene72794 | MS.gene019162 | PPI |
| MS.gene019162 | MS.gene60626 | PPI |
| MS.gene019162 | MS.gene93755 | PPI |
| MS.gene019162 | MS.gene040117 | PPI |
| MS.gene019162 | MS.gene037397 | PPI |
| MS.gene019162 | MS.gene006217 | PPI |
| MS.gene019162 | MS.gene066265 | PPI |
| MS.gene019162 | MS.gene49165 | PPI |
| MS.gene019162 | MS.gene83470 | PPI |
| MS.gene019162 | MS.gene94559 | PPI |
| MS.gene019162 | MS.gene94817 | PPI |
| MS.gene019162 | MS.gene046999 | PPI |
| MS.gene019162 | MS.gene028125 | PPI |
| MS.gene019162 | MS.gene038038 | PPI |
| MS.gene019162 | MS.gene93101 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019162.t1 | MTR_3g449990 | 97.762 | 581 | 13 | 0 | 1 | 581 | 1 | 581 | 0.0 | 1159 |
| MS.gene019162.t1 | MTR_5g090420 | 76.950 | 577 | 125 | 4 | 1 | 577 | 1 | 569 | 0.0 | 887 |
| MS.gene019162.t1 | MTR_1g069345 | 46.154 | 507 | 263 | 3 | 52 | 556 | 6 | 504 | 7.51e-161 | 469 |
| MS.gene019162.t1 | MTR_3g449950 | 68.512 | 289 | 82 | 3 | 1 | 288 | 1 | 281 | 2.14e-113 | 340 |
| MS.gene019162.t1 | MTR_1g072140 | 30.132 | 302 | 201 | 7 | 259 | 555 | 16 | 312 | 1.47e-38 | 144 |
| MS.gene019162.t1 | MTR_4g119620 | 26.667 | 315 | 217 | 8 | 253 | 555 | 9 | 321 | 4.71e-30 | 120 |
| MS.gene019162.t1 | MTR_8g092620 | 22.819 | 298 | 224 | 5 | 258 | 555 | 6 | 297 | 9.60e-22 | 96.3 |
| MS.gene019162.t1 | MTR_7g086360 | 23.179 | 302 | 215 | 10 | 263 | 554 | 48 | 342 | 4.34e-20 | 92.4 |
| MS.gene019162.t1 | MTR_8g092620 | 22.337 | 291 | 221 | 4 | 258 | 548 | 13 | 298 | 9.65e-20 | 90.9 |
| MS.gene019162.t1 | MTR_5g016350 | 26.879 | 346 | 200 | 13 | 263 | 573 | 226 | 553 | 3.53e-19 | 91.7 |
| MS.gene019162.t1 | MTR_5g016350 | 26.879 | 346 | 200 | 13 | 263 | 573 | 162 | 489 | 3.93e-19 | 91.3 |
| MS.gene019162.t1 | MTR_3g106080 | 33.143 | 175 | 110 | 4 | 268 | 439 | 459 | 629 | 1.03e-18 | 90.5 |
| MS.gene019162.t1 | MTR_5g016370 | 25.970 | 335 | 215 | 11 | 263 | 573 | 240 | 565 | 1.36e-17 | 86.7 |
| MS.gene019162.t1 | MTR_1g097140 | 26.421 | 299 | 201 | 10 | 266 | 558 | 119 | 404 | 2.00e-17 | 85.1 |
| MS.gene019162.t1 | MTR_4g095058 | 25.074 | 339 | 225 | 10 | 257 | 573 | 217 | 548 | 1.06e-16 | 84.0 |
| MS.gene019162.t1 | MTR_4g095058 | 25.074 | 339 | 225 | 10 | 257 | 573 | 215 | 546 | 1.10e-16 | 84.0 |
| MS.gene019162.t1 | MTR_1g054300 | 25.868 | 317 | 197 | 12 | 256 | 558 | 150 | 442 | 5.58e-16 | 81.3 |
| MS.gene019162.t1 | MTR_5g022690 | 29.444 | 180 | 119 | 5 | 263 | 439 | 314 | 488 | 4.65e-14 | 75.1 |
| MS.gene019162.t1 | MTR_3g061450 | 25.000 | 288 | 202 | 9 | 268 | 550 | 17 | 295 | 1.00e-13 | 72.8 |
| MS.gene019162.t1 | MTR_4g104380 | 24.522 | 314 | 209 | 11 | 255 | 555 | 215 | 513 | 1.90e-13 | 73.6 |
| MS.gene019162.t1 | MTR_6g004040 | 26.115 | 157 | 111 | 3 | 260 | 415 | 91 | 243 | 3.54e-13 | 73.2 |
| MS.gene019162.t1 | MTR_7g104550 | 24.917 | 301 | 170 | 9 | 278 | 557 | 77 | 342 | 5.00e-13 | 70.9 |
| MS.gene019162.t1 | MTR_8g467040 | 25.077 | 323 | 215 | 11 | 252 | 555 | 101 | 415 | 6.08e-13 | 71.2 |
| MS.gene019162.t1 | MTR_3g107910 | 26.761 | 284 | 188 | 12 | 277 | 555 | 635 | 903 | 2.42e-11 | 67.4 |
| MS.gene019162.t1 | MTR_3g107910 | 26.761 | 284 | 188 | 12 | 277 | 555 | 628 | 896 | 2.68e-11 | 67.0 |
| MS.gene019162.t1 | MTR_3g075400 | 23.759 | 282 | 163 | 11 | 278 | 555 | 275 | 508 | 2.92e-11 | 66.6 |
| MS.gene019162.t1 | MTR_2g012630 | 21.656 | 157 | 120 | 1 | 260 | 416 | 90 | 243 | 5.95e-11 | 66.2 |
| MS.gene019162.t1 | MTR_8g092620 | 21.154 | 208 | 161 | 2 | 258 | 465 | 6 | 210 | 6.07e-11 | 63.2 |
| MS.gene019162.t1 | MTR_1g050340 | 23.860 | 285 | 196 | 12 | 277 | 555 | 516 | 785 | 6.87e-11 | 65.9 |
| MS.gene019162.t1 | MTR_2g012630 | 21.656 | 157 | 120 | 1 | 260 | 416 | 90 | 243 | 7.20e-11 | 65.9 |
| MS.gene019162.t1 | MTR_1g050340 | 23.860 | 285 | 196 | 12 | 277 | 555 | 517 | 786 | 7.49e-11 | 65.5 |
| MS.gene019162.t1 | MTR_8g106790 | 27.835 | 194 | 130 | 5 | 372 | 557 | 31 | 222 | 9.93e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019162.t1 | AT5G08560 | 58.404 | 589 | 231 | 4 | 1 | 577 | 1 | 587 | 0.0 | 719 |
| MS.gene019162.t1 | AT5G08560 | 58.404 | 589 | 231 | 4 | 1 | 577 | 1 | 587 | 0.0 | 719 |
| MS.gene019162.t1 | AT5G08560 | 58.404 | 589 | 231 | 4 | 1 | 577 | 1 | 587 | 0.0 | 719 |
| MS.gene019162.t1 | AT5G43920 | 48.141 | 511 | 255 | 5 | 53 | 556 | 7 | 514 | 1.23e-161 | 472 |
| MS.gene019162.t1 | AT4G02730 | 31.023 | 303 | 198 | 9 | 259 | 555 | 32 | 329 | 8.34e-34 | 132 |
| MS.gene019162.t1 | AT3G49660 | 28.000 | 300 | 209 | 6 | 264 | 558 | 18 | 315 | 1.95e-31 | 124 |
| MS.gene019162.t1 | AT3G49660 | 34.328 | 134 | 83 | 3 | 261 | 391 | 146 | 277 | 1.50e-13 | 72.4 |
| MS.gene019162.t1 | AT2G43770 | 24.000 | 300 | 215 | 9 | 263 | 554 | 46 | 340 | 1.64e-23 | 102 |
| MS.gene019162.t1 | AT5G64730 | 24.585 | 301 | 219 | 6 | 256 | 555 | 4 | 297 | 1.32e-21 | 95.9 |
| MS.gene019162.t1 | AT5G25150 | 39.496 | 119 | 69 | 1 | 268 | 386 | 458 | 573 | 2.78e-19 | 92.4 |
| MS.gene019162.t1 | AT5G25150 | 23.182 | 220 | 158 | 5 | 261 | 475 | 409 | 622 | 1.01e-13 | 74.7 |
| MS.gene019162.t1 | AT5G25150 | 39.496 | 119 | 69 | 1 | 268 | 386 | 458 | 573 | 3.24e-19 | 92.0 |
| MS.gene019162.t1 | AT5G25150 | 23.182 | 220 | 158 | 5 | 261 | 475 | 409 | 622 | 1.28e-13 | 74.3 |
| MS.gene019162.t1 | AT1G71840 | 24.149 | 323 | 220 | 8 | 262 | 574 | 62 | 369 | 2.59e-16 | 81.6 |
| MS.gene019162.t1 | AT5G67320 | 24.561 | 342 | 225 | 12 | 256 | 573 | 251 | 583 | 3.67e-16 | 82.4 |
| MS.gene019162.t1 | AT2G41500 | 30.645 | 124 | 83 | 1 | 263 | 386 | 332 | 452 | 4.01e-14 | 75.9 |
| MS.gene019162.t1 | AT2G41500 | 23.232 | 297 | 178 | 6 | 260 | 555 | 288 | 535 | 3.50e-12 | 69.7 |
| MS.gene019162.t1 | AT2G41500 | 30.645 | 124 | 83 | 1 | 263 | 386 | 332 | 452 | 4.01e-14 | 75.9 |
| MS.gene019162.t1 | AT2G41500 | 23.232 | 297 | 178 | 6 | 260 | 555 | 288 | 535 | 3.50e-12 | 69.7 |
| MS.gene019162.t1 | AT1G73720 | 19.847 | 524 | 372 | 16 | 61 | 555 | 5 | 509 | 7.01e-14 | 74.7 |
| MS.gene019162.t1 | AT5G60940 | 19.792 | 384 | 269 | 11 | 208 | 555 | 45 | 425 | 2.11e-13 | 72.8 |
| MS.gene019162.t1 | AT1G10580 | 21.725 | 313 | 229 | 10 | 252 | 555 | 267 | 572 | 2.75e-13 | 73.2 |
| MS.gene019162.t1 | AT5G60940 | 20.795 | 327 | 235 | 10 | 250 | 555 | 10 | 333 | 1.50e-12 | 69.7 |
| MS.gene019162.t1 | AT5G52820 | 21.379 | 435 | 244 | 15 | 206 | 555 | 50 | 471 | 2.43e-12 | 69.7 |
| MS.gene019162.t1 | AT5G08390 | 24.359 | 156 | 115 | 1 | 260 | 415 | 91 | 243 | 6.55e-12 | 68.9 |
| MS.gene019162.t1 | AT5G54520 | 24.444 | 315 | 221 | 8 | 255 | 555 | 145 | 456 | 9.08e-12 | 67.8 |
| MS.gene019162.t1 | AT5G23430 | 23.718 | 156 | 116 | 1 | 260 | 415 | 91 | 243 | 4.08e-11 | 66.6 |
| MS.gene019162.t1 | AT5G23430 | 23.718 | 156 | 116 | 1 | 260 | 415 | 91 | 243 | 4.08e-11 | 66.6 |
| MS.gene019162.t1 | AT5G23430 | 23.718 | 156 | 116 | 1 | 260 | 415 | 91 | 243 | 4.08e-11 | 66.6 |
Find 146 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCATCTTGTTGCAAAATT+AGG | 0.127762 | 3.3:+36879321 | None:intergenic |
| TGTGGAAAGTATATACTTTC+TGG | 0.200681 | 3.3:-36874072 | MS.gene019162:CDS |
| AACCCTCAAGATTTCTGATT+TGG | 0.228555 | 3.3:-36873980 | MS.gene019162:CDS |
| GATGCGTTGCCCGGTCATTC+TGG | 0.234741 | 3.3:-36873136 | MS.gene019162:CDS |
| ATGACCGGACAGTCCGGATA+TGG | 0.290731 | 3.3:-36873054 | MS.gene019162:CDS |
| AAACAGATGAAACAGATTTC+TGG | 0.290772 | 3.3:+36874232 | None:intergenic |
| AATCTGTTTCATCTGTTTCC+TGG | 0.293813 | 3.3:-36874227 | MS.gene019162:CDS |
| AGCATCTTGTTGCAAAATTA+GGG | 0.298828 | 3.3:+36879322 | None:intergenic |
| TATTCCAAAGATGTATTTCC+TGG | 0.324628 | 3.3:+36873794 | None:intergenic |
| ATTTCTGATTTGGAAATAAC+AGG | 0.332254 | 3.3:-36873970 | MS.gene019162:CDS |
| AACGAAGTTGTTGGTTCAAA+AGG | 0.341842 | 3.3:-36879848 | MS.gene019162:CDS |
| CTAACCTGTAATGTTCTAGA+TGG | 0.343914 | 3.3:+36879227 | None:intergenic |
| TGAACCAGGAAATACATCTT+TGG | 0.345081 | 3.3:-36873798 | MS.gene019162:CDS |
| CGTTGTCCCCTTCAGATGAT+AGG | 0.345670 | 3.3:+36879867 | None:intergenic |
| GTGTCCACATTGAATAAAAT+TGG | 0.353913 | 3.3:-36879659 | MS.gene019162:CDS |
| TCCGACCAATGTGGTTCTTC+TGG | 0.354530 | 3.3:-36879458 | MS.gene019162:CDS |
| TCTTCTGTGGAGCGTGTTGT+AGG | 0.357737 | 3.3:-36879917 | MS.gene019162:CDS |
| GTAAGAAGCTCTTGGCCATT+AGG | 0.359812 | 3.3:+36874201 | None:intergenic |
| ATGATCGAACTGCAATCATT+TGG | 0.365648 | 3.3:-36875522 | MS.gene019162:CDS |
| GTTTATGAAAAGAATGGTTC+TGG | 0.368635 | 3.3:-36874120 | MS.gene019162:CDS |
| GATACCATCTAGAACATTAC+AGG | 0.375880 | 3.3:-36879231 | MS.gene019162:intron |
| GAAGGAGATCTTAAGCTTGT+TGG | 0.378421 | 3.3:-36873769 | MS.gene019162:CDS |
| ATGCCCATGACGATGAAGTA+TGG | 0.396463 | 3.3:-36875591 | MS.gene019162:CDS |
| TCTGTTTCCTGGAGTCCTAA+TGG | 0.400766 | 3.3:-36874216 | MS.gene019162:CDS |
| AAGGAGATCTTAAGCTTGTT+GGG | 0.403127 | 3.3:-36873768 | MS.gene019162:CDS |
| TACATATGGCACAGAAGCTC+AGG | 0.404511 | 3.3:-36873169 | MS.gene019162:CDS |
| ATGTTTGCTAGGTTTACATA+TGG | 0.410206 | 3.3:-36873183 | MS.gene019162:intron |
| GTTATTAGATCTTGCTTTGG+AGG | 0.410907 | 3.3:-36873715 | MS.gene019162:CDS |
| GCACAGAAGCTCAGGGGACT+TGG | 0.415521 | 3.3:-36873161 | MS.gene019162:CDS |
| TTTGTTATTAGATCTTGCTT+TGG | 0.415973 | 3.3:-36873718 | MS.gene019162:CDS |
| AAAAGATAGCAGATTCTTGT+TGG | 0.417178 | 3.3:-36873830 | MS.gene019162:CDS |
| TGAGCTTCTCGATGGGGATA+AGG | 0.418359 | 3.3:-36879576 | MS.gene019162:CDS |
| TGATCGAACTGCAATCATTT+GGG | 0.424105 | 3.3:-36875521 | MS.gene019162:CDS |
| AGCAAGATCTAATAACAAAC+CGG | 0.429270 | 3.3:+36873722 | None:intergenic |
| TCTCGATGGGGATAAGGTTA+TGG | 0.431229 | 3.3:-36879570 | MS.gene019162:CDS |
| ACGAAGGCATTGTATTCTCT+TGG | 0.432179 | 3.3:-36879791 | MS.gene019162:CDS |
| ATACATCTTTGGAATATAGA+AGG | 0.440068 | 3.3:-36873787 | MS.gene019162:CDS |
| CGAACGAAATTGTTGTGTAA+AGG | 0.440077 | 3.3:-36879965 | MS.gene019162:CDS |
| ACGGTCATCACTGCAATTGT+AGG | 0.443034 | 3.3:-36872982 | MS.gene019162:CDS |
| GAAACCATGCACAGGAGATC+AGG | 0.443605 | 3.3:+36874097 | None:intergenic |
| CTGCAAGTTTATGAAAAGAA+TGG | 0.450397 | 3.3:-36874126 | MS.gene019162:CDS |
| TGACCGGACAGTCCGGATAT+GGG | 0.451392 | 3.3:-36873053 | MS.gene019162:CDS |
| GTTTCACAACTCATTGGATA+AGG | 0.458365 | 3.3:-36879294 | MS.gene019162:CDS |
| CTCTTGGCCATTAGGACTCC+AGG | 0.461420 | 3.3:+36874209 | None:intergenic |
| ATTCCAGCTCACACAGTTAA+CGG | 0.464206 | 3.3:+36873111 | None:intergenic |
| AGCAGGTACTTGATGGTGAT+TGG | 0.465527 | 3.3:-36879691 | MS.gene019162:CDS |
| AGATGTTCCAATCTCTTCTC+AGG | 0.467671 | 3.3:+36879356 | None:intergenic |
| CTTCTATAGGTAGACACAAA+TGG | 0.468900 | 3.3:-36874288 | MS.gene019162:intron |
| AGGATCTTCGAGTGACTTGA+TGG | 0.472498 | 3.3:-36879897 | MS.gene019162:CDS |
| GTTGTCCCCTTCAGATGATA+GGG | 0.475139 | 3.3:+36879868 | None:intergenic |
| TTGCTCGTTTCACAACTCAT+TGG | 0.475980 | 3.3:-36879300 | MS.gene019162:CDS |
| GGAGGAAGCTGTTAGGCGTT+GGG | 0.478080 | 3.3:-36874169 | MS.gene019162:CDS |
| GTGGAGTGGAGGAAGCTGTT+AGG | 0.478153 | 3.3:-36874176 | MS.gene019162:CDS |
| TTATTAGATCTTGCTTTGGA+GGG | 0.480774 | 3.3:-36873714 | MS.gene019162:CDS |
| GATATGGGGCTTAAAGTGTC+TGG | 0.481401 | 3.3:-36873038 | MS.gene019162:CDS |
| CGGTCATCACTGCAATTGTA+GGG | 0.482058 | 3.3:-36872981 | MS.gene019162:CDS |
| AAGTATATACTTTCTGGCCT+CGG | 0.485551 | 3.3:-36874066 | MS.gene019162:CDS |
| AATGATACCTGAGAAGAGAT+TGG | 0.486595 | 3.3:-36879363 | MS.gene019162:CDS |
| TTTCCAAATCAGAAATCTTG+AGG | 0.489920 | 3.3:+36873977 | None:intergenic |
| ACGGGAAAGAAGTTGAGTCT+TGG | 0.492609 | 3.3:-36874014 | MS.gene019162:CDS |
| AGCCCCATATCCGGACTGTC+CGG | 0.492790 | 3.3:+36873050 | None:intergenic |
| CTTGCTGAGGCCAACATGTG+GGG | 0.492988 | 3.3:+36873079 | None:intergenic |
| TAGGCGTTGGGATGTCAGTA+CGG | 0.493450 | 3.3:-36874157 | MS.gene019162:CDS |
| TCTCAGGTATCATTACAGTC+GGG | 0.493837 | 3.3:+36879372 | None:intergenic |
| GAAAACCTAGAGGAGGAGTC+TGG | 0.494898 | 3.3:-36879752 | MS.gene019162:CDS |
| TTTATGCAGCAGGTACTTGA+TGG | 0.495020 | 3.3:-36879698 | MS.gene019162:CDS |
| CTGGCCTGATCTCCTGTGCA+TGG | 0.495260 | 3.3:-36874101 | MS.gene019162:CDS |
| GAAGGGGACAACGAAGTTGT+TGG | 0.495553 | 3.3:-36879857 | MS.gene019162:CDS |
| GACTTGGTAGATGCGTTGCC+CGG | 0.495687 | 3.3:-36873145 | MS.gene019162:CDS |
| GACACTTTAAGCCCCATATC+CGG | 0.496823 | 3.3:+36873041 | None:intergenic |
| TTCCAAATCAGAAATCTTGA+GGG | 0.500940 | 3.3:+36873978 | None:intergenic |
| CTCCACATGTAAGAAGCTCT+TGG | 0.503254 | 3.3:+36874193 | None:intergenic |
| TGGCCTCAGCAAGCGATGAC+CGG | 0.503603 | 3.3:-36873069 | MS.gene019162:CDS |
| AAGGACTCGGTTGAAACTGC+TGG | 0.505649 | 3.3:-36879417 | MS.gene019162:CDS |
| TTCTCAGGTATCATTACAGT+CGG | 0.505834 | 3.3:+36879371 | None:intergenic |
| TCCTGTGCATGGTTTCCTTG+TGG | 0.507321 | 3.3:-36874090 | MS.gene019162:CDS |
| TGGAGGAAGCTGTTAGGCGT+TGG | 0.508032 | 3.3:-36874170 | MS.gene019162:CDS |
| GCAGGTACTTGATGGTGATT+GGG | 0.509356 | 3.3:-36879690 | MS.gene019162:CDS |
| CGCTTGCTGAGGCCAACATG+TGG | 0.509685 | 3.3:+36873077 | None:intergenic |
| ACGAAGTTGTTGGTTCAAAA+GGG | 0.512756 | 3.3:-36879847 | MS.gene019162:CDS |
| GCTTGCTGAGGCCAACATGT+GGG | 0.517243 | 3.3:+36873078 | None:intergenic |
| AAGCAATCGTTTATTGCTAG+TGG | 0.517938 | 3.3:-36873688 | MS.gene019162:CDS |
| CAAGCGATGACCGGACAGTC+CGG | 0.518133 | 3.3:-36873060 | MS.gene019162:CDS |
| TTTGCATGTGGGAATTAGAC+GGG | 0.530130 | 3.3:-36874032 | MS.gene019162:CDS |
| GTTGGTTAATCTTCTGAACC+AGG | 0.530280 | 3.3:-36873812 | MS.gene019162:CDS |
| ATTTGGAAATAACAGGTGAC+GGG | 0.531109 | 3.3:-36873963 | MS.gene019162:CDS |
| GATTTGGAAATAACAGGTGA+CGG | 0.534740 | 3.3:-36873964 | MS.gene019162:CDS |
| CTCTAACGGTTCATCTTCTG+TGG | 0.542353 | 3.3:-36879930 | MS.gene019162:CDS |
| AAACCATACTTCATCGTCAT+GGG | 0.547234 | 3.3:+36875588 | None:intergenic |
| ATTTGCATGTGGGAATTAGA+CGG | 0.554899 | 3.3:-36874033 | MS.gene019162:CDS |
| ACCAGAAGAACCACATTGGT+CGG | 0.557604 | 3.3:+36879457 | None:intergenic |
| TACTCAAATGCCTACAGCAA+TGG | 0.558729 | 3.3:-36873007 | MS.gene019162:CDS |
| GTTAACGGCTCCAGAATGAC+CGG | 0.560282 | 3.3:+36873126 | None:intergenic |
| TCTATGAAGCATAAATTATG+TGG | 0.562604 | 3.3:-36874258 | MS.gene019162:CDS |
| GTATGCCAGACTCCTCCTCT+AGG | 0.567059 | 3.3:+36879747 | None:intergenic |
| GAGCCGTTAACTGTGTGAGC+TGG | 0.567883 | 3.3:-36873114 | MS.gene019162:CDS |
| TAGTGGAAGTGAGGATTCAC+AGG | 0.569174 | 3.3:-36873671 | MS.gene019162:intron |
| GCTCGGCCCCTATCATCTGA+AGG | 0.573835 | 3.3:-36879875 | MS.gene019162:CDS |
| TGGATGCATTGAAGACATTG+AGG | 0.574890 | 3.3:-36879550 | MS.gene019162:CDS |
| CTCGGCCCCTATCATCTGAA+GGG | 0.575430 | 3.3:-36879874 | MS.gene019162:CDS |
| CTTCGAGTGACTTGATGGCT+CGG | 0.576791 | 3.3:-36879892 | MS.gene019162:CDS |
| GGTAAATGTGTTTATGCAGC+AGG | 0.577455 | 3.3:-36879708 | MS.gene019162:CDS |
| AGGCGTTGGGATGTCAGTAC+GGG | 0.578210 | 3.3:-36874156 | MS.gene019162:CDS |
| GGCCAAGAGCTTCTTACATG+TGG | 0.581552 | 3.3:-36874195 | MS.gene019162:CDS |
| TGTCCGGTCATCGCTTGCTG+AGG | 0.582564 | 3.3:+36873066 | None:intergenic |
| CTCAGGTATCATTACAGTCG+GGG | 0.583773 | 3.3:+36879373 | None:intergenic |
| ATAAAAGTCATAGACGCTCC+CGG | 0.592625 | 3.3:-36873741 | MS.gene019162:CDS |
| AGTTCGATCATTTGATGCTG+TGG | 0.594560 | 3.3:+36875534 | None:intergenic |
| GTTTATTGCTAGTGGAAGTG+AGG | 0.595056 | 3.3:-36873680 | MS.gene019162:CDS |
| GAAGAACCACATTGGTCGGA+TGG | 0.599754 | 3.3:+36879461 | None:intergenic |
| GGTTGAGAGATTTATTGAAG+AGG | 0.603023 | 3.3:-36873881 | MS.gene019162:CDS |
| GCTTCTTACATGTGGAGTGG+AGG | 0.605122 | 3.3:-36874187 | MS.gene019162:CDS |
| GACCGGACAGTCCGGATATG+GGG | 0.607301 | 3.3:-36873052 | MS.gene019162:CDS |
| CATACCGTTGAATTCTTCTG+TGG | 0.607582 | 3.3:-36879729 | MS.gene019162:CDS |
| GTGACAAAAGCATTTGCATG+TGG | 0.612110 | 3.3:-36874044 | MS.gene019162:CDS |
| TGACAAAAGCATTTGCATGT+GGG | 0.613809 | 3.3:-36874043 | MS.gene019162:CDS |
| CAAACCATACTTCATCGTCA+TGG | 0.614532 | 3.3:+36875587 | None:intergenic |
| TCCACAAGGAAACCATGCAC+AGG | 0.616331 | 3.3:+36874089 | None:intergenic |
| AGAGCTTCTTACATGTGGAG+TGG | 0.628710 | 3.3:-36874190 | MS.gene019162:CDS |
| ACATATGGCACAGAAGCTCA+GGG | 0.629533 | 3.3:-36873168 | MS.gene019162:CDS |
| TTGCTGAGGCCAACATGTGG+GGG | 0.630797 | 3.3:+36873080 | None:intergenic |
| GCTCTTCAATAAAGAAACGA+AGG | 0.630901 | 3.3:-36873902 | MS.gene019162:CDS |
| AAAGAAGTTGAGTCTTGGAA+AGG | 0.635577 | 3.3:-36874009 | MS.gene019162:CDS |
| GGATTAAACAGTCTCTCTAA+CGG | 0.639200 | 3.3:-36879944 | MS.gene019162:CDS |
| TTGTCCCCTTCAGATGATAG+GGG | 0.640840 | 3.3:+36879869 | None:intergenic |
| GGTACTTGATGGTGATTGGG+AGG | 0.643190 | 3.3:-36879687 | MS.gene019162:CDS |
| TTTACCACAGAAGAATTCAA+CGG | 0.644026 | 3.3:+36879725 | None:intergenic |
| ATGACCGTTGCCATTGCTGT+AGG | 0.644794 | 3.3:+36872997 | None:intergenic |
| GCAAGATCTAATAACAAACC+GGG | 0.647814 | 3.3:+36873723 | None:intergenic |
| AATGCCTACAGCAATGGCAA+CGG | 0.648036 | 3.3:-36873001 | MS.gene019162:CDS |
| GACTCGGTTGAAACTGCTGG+AGG | 0.656658 | 3.3:-36879414 | MS.gene019162:CDS |
| TCGGCCCCTATCATCTGAAG+GGG | 0.658928 | 3.3:-36879873 | MS.gene019162:CDS |
| GACCGGGGAAAACCTAGAGG+AGG | 0.668456 | 3.3:-36879759 | MS.gene019162:CDS |
| TTGTCACCATCCGACCAATG+TGG | 0.673046 | 3.3:-36879467 | MS.gene019162:CDS |
| CTCGACCAGAAGAACCACAT+TGG | 0.680833 | 3.3:+36879453 | None:intergenic |
| TCGAACTGCAATCATTTGGG+AGG | 0.684790 | 3.3:-36875518 | MS.gene019162:intron |
| TCAGGTATCATTACAGTCGG+GGG | 0.685221 | 3.3:+36879374 | None:intergenic |
| TTAACGGCTCCAGAATGACC+GGG | 0.689998 | 3.3:+36873127 | None:intergenic |
| CTATAGGTAGACACAAATGG+TGG | 0.695509 | 3.3:-36874285 | MS.gene019162:intron |
| GGTCATCACTGCAATTGTAG+GGG | 0.702846 | 3.3:-36872980 | MS.gene019162:CDS |
| GAAAGTATATACTTTCCACA+AGG | 0.711786 | 3.3:+36874075 | None:intergenic |
| AAAGACCGGGGAAAACCTAG+AGG | 0.721392 | 3.3:-36879762 | MS.gene019162:CDS |
| GTTTGTAAGGATAATCACGA+AGG | 0.736409 | 3.3:-36879807 | MS.gene019162:CDS |
| CATATGGCACAGAAGCTCAG+GGG | 0.737243 | 3.3:-36873167 | MS.gene019162:CDS |
| TTTGGAAATAACAGGTGACG+GGG | 0.738612 | 3.3:-36873962 | MS.gene019162:CDS |
| TTATATTCAGATCATCACTG+TGG | 0.742830 | 3.3:-36879263 | MS.gene019162:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 36872973 | 36880023 | 36872973 | ID=MS.gene019162 |
| chr3.3 | mRNA | 36872973 | 36880023 | 36872973 | ID=MS.gene019162.t1;Parent=MS.gene019162 |
| chr3.3 | exon | 36879232 | 36880023 | 36879232 | ID=MS.gene019162.t1.exon1;Parent=MS.gene019162.t1 |
| chr3.3 | CDS | 36879232 | 36880023 | 36879232 | ID=cds.MS.gene019162.t1;Parent=MS.gene019162.t1 |
| chr3.3 | exon | 36875519 | 36875620 | 36875519 | ID=MS.gene019162.t1.exon2;Parent=MS.gene019162.t1 |
| chr3.3 | CDS | 36875519 | 36875620 | 36875519 | ID=cds.MS.gene019162.t1;Parent=MS.gene019162.t1 |
| chr3.3 | exon | 36873672 | 36874301 | 36873672 | ID=MS.gene019162.t1.exon3;Parent=MS.gene019162.t1 |
| chr3.3 | CDS | 36873672 | 36874301 | 36873672 | ID=cds.MS.gene019162.t1;Parent=MS.gene019162.t1 |
| chr3.3 | exon | 36872973 | 36873194 | 36872973 | ID=MS.gene019162.t1.exon4;Parent=MS.gene019162.t1 |
| chr3.3 | CDS | 36872973 | 36873194 | 36872973 | ID=cds.MS.gene019162.t1;Parent=MS.gene019162.t1 |
| Gene Sequence |
| Protein sequence |
