AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene017032

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017032.t1 MTR_5g090420 98.427 572 9 0 1 572 1 572 0.0 1157
MS.gene017032.t1 MTR_3g449990 76.764 581 126 5 1 572 1 581 0.0 872
MS.gene017032.t1 MTR_1g069345 47.400 500 258 3 53 549 7 504 1.06e-162 473
MS.gene017032.t1 MTR_3g449950 56.747 289 110 5 1 282 1 281 3.49e-88 275
MS.gene017032.t1 MTR_1g072140 29.900 301 202 7 253 548 16 312 1.35e-38 144
MS.gene017032.t1 MTR_4g119620 26.948 308 211 7 253 548 16 321 2.88e-29 118
MS.gene017032.t1 MTR_4g119620 27.536 138 96 2 250 384 148 284 3.09e-11 65.5
MS.gene017032.t1 MTR_8g092620 24.242 297 220 5 252 548 6 297 5.37e-24 102
MS.gene017032.t1 MTR_8g092620 24.068 295 219 5 252 546 13 302 8.80e-22 97.1
MS.gene017032.t1 MTR_1g063790 41.748 103 15 1 403 505 3 60 1.03e-19 83.6
MS.gene017032.t1 MTR_7g086360 22.742 299 219 8 257 547 48 342 4.83e-19 89.4
MS.gene017032.t1 MTR_5g016350 25.915 328 198 11 257 551 162 477 1.02e-16 83.6
MS.gene017032.t1 MTR_5g016350 27.586 174 112 5 257 420 347 516 1.98e-12 70.1
MS.gene017032.t1 MTR_5g016350 26.506 332 191 12 257 551 226 541 1.05e-16 84.0
MS.gene017032.t1 MTR_5g016350 27.011 174 113 4 257 420 411 580 1.48e-12 70.9
MS.gene017032.t1 MTR_1g054300 27.124 306 185 13 263 552 160 443 1.88e-16 82.8
MS.gene017032.t1 MTR_5g016370 25.382 327 199 10 257 551 240 553 1.96e-16 83.2
MS.gene017032.t1 MTR_5g016370 27.011 174 113 4 257 420 423 592 9.83e-12 68.2
MS.gene017032.t1 MTR_5g016370 23.305 236 153 8 343 559 240 466 3.35e-11 66.2
MS.gene017032.t1 MTR_3g106080 31.214 173 111 5 263 431 460 628 2.99e-16 82.8
MS.gene017032.t1 MTR_4g095058 24.159 327 211 8 252 550 218 535 6.69e-15 78.2
MS.gene017032.t1 MTR_4g095058 24.159 327 211 8 252 550 216 533 7.87e-15 77.8
MS.gene017032.t1 MTR_5g022690 28.095 210 142 6 226 430 281 486 1.22e-13 73.9
MS.gene017032.t1 MTR_4g102520 21.338 314 234 8 243 548 276 584 7.51e-13 71.6
MS.gene017032.t1 MTR_3g061450 24.390 287 202 9 263 543 18 295 1.40e-12 69.3
MS.gene017032.t1 MTR_8g038550 22.222 297 178 10 259 551 220 467 1.73e-12 70.9
MS.gene017032.t1 MTR_7g104550 23.659 317 179 8 261 552 66 344 2.75e-12 68.6
MS.gene017032.t1 MTR_6g004040 25.000 160 116 3 250 408 87 243 5.39e-12 69.3
MS.gene017032.t1 MTR_3g052220 22.911 371 243 16 208 548 154 511 9.60e-12 67.8
MS.gene017032.t1 MTR_3g075400 21.908 283 170 9 270 548 273 508 2.77e-11 66.6
MS.gene017032.t1 MTR_8g092620 21.739 207 160 2 252 458 6 210 2.83e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017032.t1 AT5G08560 59.492 590 217 8 1 570 1 588 0.0 712
MS.gene017032.t1 AT5G08560 59.492 590 217 8 1 570 1 588 0.0 712
MS.gene017032.t1 AT5G08560 59.492 590 217 8 1 570 1 588 0.0 712
MS.gene017032.t1 AT5G43920 48.544 515 252 6 53 555 7 520 2.00e-165 481
MS.gene017032.t1 AT4G02730 32.013 303 194 10 253 548 32 329 3.07e-34 132
MS.gene017032.t1 AT3G49660 26.974 304 214 5 251 548 11 312 4.36e-30 120
MS.gene017032.t1 AT3G49660 31.159 138 91 2 250 384 141 277 8.50e-14 73.2
MS.gene017032.t1 AT2G43770 24.415 299 214 8 257 547 46 340 2.53e-23 102
MS.gene017032.t1 AT5G64730 24.916 297 218 5 252 548 6 297 1.11e-22 99.0
MS.gene017032.t1 AT5G56130 25.472 318 211 12 252 552 6 314 2.02e-17 84.0
MS.gene017032.t1 AT5G25150 36.441 118 71 2 263 379 459 573 3.74e-17 85.5
MS.gene017032.t1 AT5G25150 36.441 118 71 2 263 379 459 573 3.88e-17 85.5
MS.gene017032.t1 AT5G67320 24.169 331 206 11 252 550 253 570 1.12e-14 77.4
MS.gene017032.t1 AT1G71840 21.725 313 199 7 256 565 62 331 1.07e-13 73.6
MS.gene017032.t1 AT3G27640 24.567 289 171 9 306 550 126 411 5.04e-13 72.0
MS.gene017032.t1 AT1G10580 22.186 311 229 8 246 548 267 572 5.32e-13 72.0
MS.gene017032.t1 AT3G27640 24.567 289 171 9 306 550 107 392 6.08e-13 72.0
MS.gene017032.t1 AT5G13480 21.886 297 179 10 259 551 171 418 1.08e-12 71.2
MS.gene017032.t1 AT5G13480 21.886 297 179 10 259 551 148 395 1.37e-12 70.9
MS.gene017032.t1 AT5G13480 21.886 297 179 10 259 551 160 407 1.42e-12 70.9
MS.gene017032.t1 AT5G13480 21.886 297 179 10 259 551 148 395 1.53e-12 70.9
MS.gene017032.t1 AT5G52820 23.121 346 208 11 250 548 137 471 1.78e-12 70.1
MS.gene017032.t1 AT5G52820 26.056 142 104 1 279 420 332 472 7.27e-11 65.1
MS.gene017032.t1 AT2G41500 22.143 280 166 7 272 548 263 493 3.58e-12 69.3
MS.gene017032.t1 AT2G41500 23.423 222 124 5 257 435 332 550 1.07e-11 68.2
MS.gene017032.t1 AT2G41500 22.143 280 166 7 272 548 263 493 3.58e-12 69.3
MS.gene017032.t1 AT2G41500 23.423 222 124 5 257 435 332 550 1.07e-11 68.2
MS.gene017032.t1 AT5G16750 30.189 159 101 6 400 554 27 179 5.90e-12 69.3
MS.gene017032.t1 AT1G73720 20.152 526 361 21 61 548 5 509 5.97e-11 65.5
MS.gene017032.t1 AT2G32700 22.615 283 199 11 272 548 518 786 8.46e-11 65.5
MS.gene017032.t1 AT2G32700 22.615 283 199 11 272 548 518 786 8.46e-11 65.5
MS.gene017032.t1 AT2G32700 22.615 283 199 11 272 548 518 786 8.46e-11 65.5
MS.gene017032.t1 AT2G32700 22.615 283 199 11 272 548 518 786 8.46e-11 65.5
MS.gene017032.t1 AT2G32700 22.615 283 199 11 272 548 518 786 8.46e-11 65.5
MS.gene017032.t1 AT2G32700 22.615 283 199 11 272 548 537 805 8.93e-11 65.5
MS.gene017032.t1 AT2G32700 22.615 283 199 11 272 548 516 784 9.13e-11 65.5

Find 161 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCCAAAGATTAATTTCT+TGG 0.164363 5.1:-71949261 None:intergenic
AAATTCTTTGAACTTCTAAA+TGG 0.177095 5.1:+71947000 MS.gene017032:CDS
ATCGCTTAAGATTTCTGATT+TGG 0.214294 5.1:+71949075 MS.gene017032:CDS
AACCCATTTCTTCTGTTTCA+TGG 0.245339 5.1:+71948828 MS.gene017032:CDS
AAACAGAAGAAATGGGTTTC+TGG 0.247488 5.1:-71948823 None:intergenic
AGCCCTTACCTTGCAGTTAG+AGG 0.290376 5.1:+71947245 MS.gene017032:CDS
AGTGGGAAACATATACTTTC+TGG 0.294428 5.1:+71948983 MS.gene017032:CDS
AACAAGTACTTGATGGGAAT+TGG 0.300522 5.1:+71946893 MS.gene017032:CDS
TTGAACAGCAATACTGTATT+GGG 0.302367 5.1:-71949139 None:intergenic
ATGACCGTACAATTCGGATA+TGG 0.302803 5.1:+71949876 MS.gene017032:CDS
TGAACCAAGAAATTAATCTT+TGG 0.323203 5.1:+71949257 MS.gene017032:CDS
GGCGCTCGAGGTTTATTATA+AGG 0.334980 5.1:+71949326 MS.gene017032:CDS
GCAGGGATAGTAGTAGAATT+CGG 0.341628 5.1:+71947076 MS.gene017032:CDS
ATTGAACAGCAATACTGTAT+TGG 0.342346 5.1:-71949140 None:intergenic
AGGAGATTACTCCACTTTGC+AGG 0.342931 5.1:+71947058 MS.gene017032:CDS
GAGGCACTGCCTGGTCATTC+AGG 0.344214 5.1:+71949794 MS.gene017032:CDS
GTCGGCCGCGTGTTTGATAT+TGG 0.346912 5.1:+71946972 MS.gene017032:CDS
ATGATCAAACTGCAATCATC+TGG 0.349518 5.1:+71948489 MS.gene017032:CDS
CTGGCTTAGTCTCCTGTACA+TGG 0.354065 5.1:+71948954 MS.gene017032:CDS
GATTTCTGATTTGGATATAA+CGG 0.356904 5.1:+71949084 MS.gene017032:CDS
ACTCCATGAAACAGAAGAAA+TGG 0.360475 5.1:-71948831 None:intergenic
TAAAGAGAGACGAGTTTGTT+AGG 0.364649 5.1:+71946764 MS.gene017032:CDS
TGAAAAGAGGTTGGAACATC+TGG 0.366917 5.1:+71947215 MS.gene017032:CDS
ATTTGCATGTGGGAATTAGA+TGG 0.371375 5.1:+71949022 MS.gene017032:CDS
CTAACCTGTACTGTTCTAGA+TGG 0.373437 5.1:-71947339 None:intergenic
AATTCTTTGAACTTCTAAAT+GGG 0.379699 5.1:+71947001 MS.gene017032:CDS
GTCTCTCTTTATAACCCCTT+TGG 0.381651 5.1:-71946752 None:intergenic
GTGAGGAGCTCTTCGTCATT+AGG 0.382343 5.1:-71948854 None:intergenic
CAGGGATAGTAGTAGAATTC+GGG 0.384222 5.1:+71947077 MS.gene017032:CDS
TTTGAAACGTATGAAGGTTT+CGG 0.391023 5.1:+71946612 MS.gene017032:CDS
CTTCTTTGTAGGTCTACATA+TGG 0.393962 5.1:+71949747 MS.gene017032:intron
ATTATAAGGTCTTGCTTTGG+TGG 0.406114 5.1:+71949340 MS.gene017032:CDS
GTGGAGTGGAGGAATCTATT+AGG 0.413571 5.1:+71948879 MS.gene017032:CDS
GCGCGGCTAGAGGAGGAGTC+TGG 0.418206 5.1:+71946832 MS.gene017032:CDS
ATAAATTTGGAGACACTTGC+CGG 0.419366 5.1:-71948918 None:intergenic
TTTATTATAAGGTCTTGCTT+TGG 0.419821 5.1:+71949337 MS.gene017032:CDS
CGTGTAAAGCATAGATTATC+TGG 0.420014 5.1:+71948797 MS.gene017032:CDS
GCTAACATGTGGGGATTAGC+TGG 0.424867 5.1:-71949842 None:intergenic
TTTACGCAACAAGTACTTGA+TGG 0.428247 5.1:+71946886 MS.gene017032:CDS
TTGTACTTCCCAATGAGCTT+AGG 0.432048 5.1:-71949295 None:intergenic
GTAGCTACATTGCGTAAGAT+TGG 0.437473 5.1:+71946925 MS.gene017032:CDS
GTTTCGGCGAAAGGATTAGT+TGG 0.438274 5.1:+71946628 MS.gene017032:CDS
ACCGTCCGAAGGGGACGATC+AGG 0.440225 5.1:+71946720 MS.gene017032:CDS
ACCTTCATACGTTTCAAAGC+TGG 0.443185 5.1:-71946607 None:intergenic
TACATATGGCACCGAAGCTC+AGG 0.443665 5.1:+71949761 MS.gene017032:CDS
GATTAGTTGGATTGTCAAAC+GGG 0.445596 5.1:+71946641 MS.gene017032:CDS
TTGCCCATTTCACAACTCAT+TGG 0.447546 5.1:+71947269 MS.gene017032:CDS
TGATCCAATGAGTTGTGAAA+TGG 0.449737 5.1:-71947273 None:intergenic
TGACCGTACAATTCGGATAT+GGG 0.450477 5.1:+71949877 MS.gene017032:CDS
ATTAATCTTTGGAATATTGA+AGG 0.452243 5.1:+71949268 MS.gene017032:CDS
ACCTTTACAATATCTTGCTT+AGG 0.458978 5.1:-71947129 None:intergenic
ACAAGTACTTGATGGGAATT+GGG 0.460130 5.1:+71946894 MS.gene017032:CDS
GAAGGCGATCCTAAGCTCAT+TGG 0.460407 5.1:+71949286 MS.gene017032:CDS
CGTATGAAGGTTTCGGCGAA+AGG 0.461320 5.1:+71946619 MS.gene017032:CDS
ATGGGAAAGAAGTGGAGTCT+TGG 0.463584 5.1:+71949041 MS.gene017032:CDS
AAGTTCCTCCATTGCAGTAA+TGG 0.463877 5.1:-71949949 None:intergenic
AAGCCTCTAACTGCAAGGTA+AGG 0.469272 5.1:-71947248 None:intergenic
GGATTAGTTGGATTGTCAAA+CGG 0.473578 5.1:+71946640 MS.gene017032:CDS
CTATCGGCTCTCCTGAGCTT+CGG 0.473900 5.1:-71949772 None:intergenic
AAGGCGATCCTAAGCTCATT+GGG 0.475240 5.1:+71949287 MS.gene017032:CDS
GGAGGAATCTATTAGGCGTT+GGG 0.480100 5.1:+71948886 MS.gene017032:CDS
CCAGTGATGACCGTACAATT+CGG 0.481345 5.1:+71949870 MS.gene017032:CDS
ACCAGCTTTGAAACGTATGA+AGG 0.489309 5.1:+71946606 MS.gene017032:CDS
TTGGGAAGTACAAAGGTCAT+AGG 0.494292 5.1:+71949305 MS.gene017032:CDS
GGAATACCTTTGCACTCACC+TGG 0.496485 5.1:+71946853 MS.gene017032:CDS
ACAATATCTTGCTTAGGAGA+TGG 0.500640 5.1:-71947123 None:intergenic
ATTTGGATATAACGGCTGAC+GGG 0.500965 5.1:+71949092 MS.gene017032:CDS
TCAGGTATCATCACTGTTGG+AGG 0.502498 5.1:-71947195 None:intergenic
GCAAAGGCATTGTATTCGCT+TGG 0.504088 5.1:+71946793 MS.gene017032:CDS
TCACTTGAGAAACTTCCAAC+AGG 0.504215 5.1:-71946679 None:intergenic
CCGAATTGTACGGTCATCAC+TGG 0.507746 5.1:-71949870 None:intergenic
ATCAGGTTGTCGGTTCCAAA+GGG 0.509238 5.1:+71946737 MS.gene017032:CDS
AGGCGTTGGGATGTTTCTAC+CGG 0.511324 5.1:+71948899 MS.gene017032:CDS
TGGAGGAATCTATTAGGCGT+TGG 0.513191 5.1:+71948885 MS.gene017032:CDS
GACAACCTGATCGTCCCCTT+CGG 0.518549 5.1:-71946725 None:intergenic
CTCCATGAAACAGAAGAAAT+GGG 0.519066 5.1:-71948830 None:intergenic
GATCCAATGAGTTGTGAAAT+GGG 0.522742 5.1:-71947272 None:intergenic
GATCAGGTTGTCGGTTCCAA+AGG 0.527948 5.1:+71946736 MS.gene017032:CDS
TTACGCAACAAGTACTTGAT+GGG 0.533338 5.1:+71946887 MS.gene017032:CDS
AAGTTTCTCAAGTGACTCCA+TGG 0.534561 5.1:+71946687 MS.gene017032:CDS
GAAAGTATATGTTTCCCACT+TGG 0.535435 5.1:-71948980 None:intergenic
GGAAGCATTGAAGACCTTGA+GGG 0.536828 5.1:+71947035 MS.gene017032:CDS
GAAGGGGACGATCAGGTTGT+CGG 0.536973 5.1:+71946727 MS.gene017032:CDS
ATTCTTTGAACTTCTAAATG+GGG 0.538918 5.1:+71947002 MS.gene017032:CDS
AAGCTCAGGAGAGCCGATAG+AGG 0.539387 5.1:+71949775 MS.gene017032:CDS
TGACAGATGACACTATTGTC+AGG 0.541344 5.1:+71946950 MS.gene017032:CDS
TTTGCATGTGGGAATTAGAT+GGG 0.543867 5.1:+71949023 MS.gene017032:CDS
CAGTGATGATACCTGAAAAG+AGG 0.546109 5.1:+71947202 MS.gene017032:CDS
GTGATAAGAGCATTTGCATG+TGG 0.550089 5.1:+71949011 MS.gene017032:CDS
TTCTTTGAACTTCTAAATGG+GGG 0.550524 5.1:+71947003 MS.gene017032:CDS
CTCGGCTACTACCGTCCGAA+GGG 0.555270 5.1:+71946710 MS.gene017032:CDS
GATTTGGATATAACGGCTGA+CGG 0.556339 5.1:+71949091 MS.gene017032:CDS
GATGATACCTGAAAAGAGGT+TGG 0.556463 5.1:+71947206 MS.gene017032:CDS
GAGTGGGGCGCGGCTAGAGG+AGG 0.557951 5.1:+71946825 MS.gene017032:CDS
AAAGAGTGGGGCGCGGCTAG+AGG 0.558493 5.1:+71946822 MS.gene017032:CDS
GGACGGTAGTAGCCGAGCCA+TGG 0.559268 5.1:-71946704 None:intergenic
CCTGTACATGGTTTCCAAGT+GGG 0.561775 5.1:+71948966 MS.gene017032:CDS
GGAGATTACTCCACTTTGCA+GGG 0.564765 5.1:+71947059 MS.gene017032:CDS
TCGCTTGGTTATAGAAAGAG+TGG 0.565963 5.1:+71946808 MS.gene017032:CDS
GAGCAAGCTTTCGTTGCAAG+TGG 0.568125 5.1:+71949367 MS.gene017032:CDS
AAACACATGATGATGAGGTA+TGG 0.568303 5.1:+71948420 MS.gene017032:CDS
AAGTGGAAGTGAGGATTCAC+AGG 0.569174 5.1:+71949384 MS.gene017032:CDS
GAGCCGATAGAGGCACTGCC+TGG 0.571510 5.1:+71949785 MS.gene017032:CDS
CCCACTTGGAAACCATGTAC+AGG 0.572154 5.1:-71948966 None:intergenic
GCTCGGCTACTACCGTCCGA+AGG 0.572516 5.1:+71946709 MS.gene017032:CDS
TGATCAAACTGCAATCATCT+GGG 0.573879 5.1:+71948490 MS.gene017032:CDS
AGCCTCTAACTGCAAGGTAA+GGG 0.576761 5.1:-71947247 None:intergenic
TCCTAAGCAAGATATTGTAA+AGG 0.577339 5.1:+71947128 MS.gene017032:CDS
GTTTGTTAGGATAATTGCAA+AGG 0.580074 5.1:+71946777 MS.gene017032:CDS
ACTGGCAGAGGCTAACATGT+GGG 0.581947 5.1:-71949852 None:intergenic
GATACCATCTAGAACAGTAC+AGG 0.582230 5.1:+71947335 MS.gene017032:CDS
TGGAGTAATCTCCTCCCTCA+AGG 0.585707 5.1:-71947049 None:intergenic
TCTTCTTCTAAAGAGCCTGT+TGG 0.587005 5.1:+71946664 MS.gene017032:CDS
ATAAAGAAACAAAGGATGAG+AGG 0.587569 5.1:+71949161 MS.gene017032:CDS
GGCATCCATTACTGCAATGG+AGG 0.591892 5.1:+71949944 MS.gene017032:CDS
GGAATTAGATGGGAAAGAAG+TGG 0.592587 5.1:+71949033 MS.gene017032:CDS
TTTCGTTGCAAGTGGAAGTG+AGG 0.593078 5.1:+71949375 MS.gene017032:CDS
CGCTTGGTTATAGAAAGAGT+GGG 0.593369 5.1:+71946809 MS.gene017032:CDS
TGTACGGTCATCACTGGCAG+AGG 0.595492 5.1:-71949864 None:intergenic
GCTGTTCAATAAAGAAACAA+AGG 0.597283 5.1:+71949153 MS.gene017032:CDS
GACGAAGAGCTCCTCACATG+TGG 0.597893 5.1:+71948860 MS.gene017032:CDS
GCTCCTCACATGTGGAGTGG+AGG 0.600384 5.1:+71948868 MS.gene017032:CDS
ATATTGTAAAGGTGAGATCT+CGG 0.600496 5.1:+71947139 MS.gene017032:CDS
TGCCATTGCTGTGCGCATGT+TGG 0.601143 5.1:-71949925 None:intergenic
ACCTGATCGTCCCCTTCGGA+CGG 0.603999 5.1:-71946721 None:intergenic
AGGAGATGGAGACACTAAGC+AGG 0.606908 5.1:-71947109 None:intergenic
TCTCAAGTGACTCCATGGCT+CGG 0.607266 5.1:+71946692 MS.gene017032:CDS
TTCAGATCATCATTGTGGAA+AGG 0.608588 5.1:+71947308 MS.gene017032:CDS
CTTAGAAACACATGATGATG+AGG 0.609621 5.1:+71948415 MS.gene017032:CDS
GAGCTGTTAATTGTGTGAGC+TGG 0.609857 5.1:+71949816 MS.gene017032:CDS
TTATATTCAGATCATCATTG+TGG 0.610290 5.1:+71947303 MS.gene017032:CDS
TGGAAGCATTGAAGACCTTG+AGG 0.611400 5.1:+71947034 MS.gene017032:CDS
TGACCAGGCAGTGCCTCTAT+CGG 0.611974 5.1:-71949788 None:intergenic
AATGGCATCCATTACTGCAA+TGG 0.618205 5.1:+71949941 MS.gene017032:CDS
GGCCCCATATCCGAATTGTA+CGG 0.618933 5.1:-71949880 None:intergenic
TGATAAGAGCATTTGCATGT+GGG 0.619083 5.1:+71949012 MS.gene017032:CDS
ATCTCGGTCAAAGCTTCTAG+AGG 0.620596 5.1:+71947155 MS.gene017032:CDS
AAGCTCATTGGGAAGTACAA+AGG 0.624344 5.1:+71949298 MS.gene017032:CDS
GACCGTACAATTCGGATATG+GGG 0.628939 5.1:+71949878 MS.gene017032:CDS
AGAGCTCCTCACATGTGGAG+TGG 0.631004 5.1:+71948865 MS.gene017032:CDS
CACTGGCAGAGGCTAACATG+TGG 0.632424 5.1:-71949853 None:intergenic
CTGGCAGAGGCTAACATGTG+GGG 0.632842 5.1:-71949851 None:intergenic
TTTGGATATAACGGCTGACG+GGG 0.633962 5.1:+71949093 MS.gene017032:CDS
ACAAAGGTCATAGGCGCTCG+AGG 0.635818 5.1:+71949314 MS.gene017032:CDS
AAAGAAGTGGAGTCTTGGAA+AGG 0.636859 5.1:+71949046 MS.gene017032:CDS
TGGGCAAGCCTCTAACTGCA+AGG 0.638184 5.1:-71947253 None:intergenic
TTGACACCAGGTGAGTGCAA+AGG 0.639008 5.1:-71946859 None:intergenic
TCCTGTACATGGTTTCCAAG+TGG 0.639029 5.1:+71948965 MS.gene017032:CDS
TTAACAGCTCCTGAATGACC+AGG 0.643672 5.1:-71949803 None:intergenic
AGATGACACTATTGTCAGGT+CGG 0.645072 5.1:+71946954 MS.gene017032:CDS
CTACTACTATCCCTGCAAAG+TGG 0.645119 5.1:-71947069 None:intergenic
TACCAACATGCGCACAGCAA+TGG 0.645886 5.1:+71949923 MS.gene017032:CDS
ATTCCTCCACTCCACATGTG+AGG 0.658511 5.1:-71948871 None:intergenic
AGCATTGAAGACCTTGAGGG+AGG 0.665437 5.1:+71947038 MS.gene017032:CDS
TGCGTAAACAGATTGACACC+AGG 0.666046 5.1:-71946871 None:intergenic
CTGTTCCAATATCAAACACG+CGG 0.669040 5.1:-71946977 None:intergenic
GTTATAGAAAGAGTGGGGCG+CGG 0.677569 5.1:+71946815 MS.gene017032:CDS
TCGGCTACTACCGTCCGAAG+GGG 0.678925 5.1:+71946711 MS.gene017032:CDS
TCAGGTTGTCGGTTCCAAAG+GGG 0.696509 5.1:+71946738 MS.gene017032:CDS
GCTTGGTTATAGAAAGAGTG+GGG 0.698893 5.1:+71946810 MS.gene017032:CDS
TCAAACTGCAATCATCTGGG+AGG 0.712903 5.1:+71948493 MS.gene017032:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATTAAAAATAATATAGAT+AGG - chr5.1:71948208-71948227 None:intergenic 10.0%
!!! TTTATAGAATTTTTACTATA+AGG - chr5.1:71948102-71948121 None:intergenic 10.0%
!! ATTAGATATAAAATGTAGTT+TGG + chr5.1:71949583-71949602 MS.gene017032:intron 15.0%
!!! AAAGGATAATAAATTTTTGT+TGG + chr5.1:71949225-71949244 MS.gene017032:CDS 15.0%
!!! TTTGCAAAATAAAATTCTTT+TGG + chr5.1:71949467-71949486 MS.gene017032:intron 15.0%
!! AAATTCTTTGAACTTCTAAA+TGG + chr5.1:71947000-71947019 MS.gene017032:CDS 20.0%
!! AAGAAAAAGAAGTTATTGTT+TGG - chr5.1:71949201-71949220 None:intergenic 20.0%
!! AATTCTTTGAACTTCTAAAT+GGG + chr5.1:71947001-71947020 MS.gene017032:CDS 20.0%
!! ATTATGTGGATAATAAATTG+TGG + chr5.1:71947599-71947618 MS.gene017032:intron 20.0%
!! TATTCCAAAGATTAATTTCT+TGG - chr5.1:71949264-71949283 None:intergenic 20.0%
!! TTTCCAAAATCTATCATAAT+TGG - chr5.1:71947756-71947775 None:intergenic 20.0%
!!! ACTTTTTATTCAGACTATTA+AGG - chr5.1:71949904-71949923 None:intergenic 20.0%
!!! AGTAAATATGGATTTTCTAA+AGG - chr5.1:71949528-71949547 None:intergenic 20.0%
!!! ATTAATCTTTGGAATATTGA+AGG + chr5.1:71949268-71949287 MS.gene017032:CDS 20.0%
!!! ATTTTTGGTCTTCTTTTTAA+AGG + chr5.1:71948757-71948776 MS.gene017032:intron 20.0%
!!! GGGTTTTAGATTTTAATTTT+TGG + chr5.1:71948742-71948761 MS.gene017032:intron 20.0%
!!! TACATTTTATATCTAATCTC+AGG - chr5.1:71949580-71949599 None:intergenic 20.0%
!!! TATCCAATTATGATAGATTT+TGG + chr5.1:71947750-71947769 MS.gene017032:intron 20.0%
!!! TTGTACAATTTTCACATAAT+GGG + chr5.1:71948444-71948463 MS.gene017032:CDS 20.0%
!!! TTTGTACAATTTTCACATAA+TGG + chr5.1:71948443-71948462 MS.gene017032:CDS 20.0%
! AAAATTATGTTGTGATGTAC+TGG + chr5.1:71947576-71947595 MS.gene017032:intron 25.0%
! AACTAGACAAAATCTTCTAT+TGG + chr5.1:71947833-71947852 MS.gene017032:intron 25.0%
! ATCTGAGTTCACTAAATTAA+TGG + chr5.1:71947804-71947823 MS.gene017032:intron 25.0%
! ATTCTTTGAACTTCTAAATG+GGG + chr5.1:71947002-71947021 MS.gene017032:CDS 25.0%
! GAAATTGCGATATTTATTGA+TGG + chr5.1:71947445-71947464 MS.gene017032:intron 25.0%
! TACAAGATATTATCCTTCAA+AGG - chr5.1:71949446-71949465 None:intergenic 25.0%
! TATCACTTTATGTCTTACAA+GGG + chr5.1:71949502-71949521 MS.gene017032:intron 25.0%
! TGAACCAAGAAATTAATCTT+TGG + chr5.1:71949257-71949276 MS.gene017032:CDS 25.0%
! TGAAGGAAAAAATTACAACT+AGG - chr5.1:71948621-71948640 None:intergenic 25.0%
! TTATATTCAGATCATCATTG+TGG + chr5.1:71947303-71947322 MS.gene017032:CDS 25.0%
! TTATCACTTTATGTCTTACA+AGG + chr5.1:71949501-71949520 MS.gene017032:intron 25.0%
! TTTATTATAAGGTCTTGCTT+TGG + chr5.1:71949337-71949356 MS.gene017032:CDS 25.0%
!! AACTTCTTTTTCTTTGTCAA+AGG + chr5.1:71949207-71949226 MS.gene017032:CDS 25.0%
!! ATCCATATTTACTTGTTTCT+AGG + chr5.1:71949535-71949554 MS.gene017032:intron 25.0%
!!! AACCAGCTTTTTCATAAATT+TGG - chr5.1:71948934-71948953 None:intergenic 25.0%
!!! GATTTCTGATTTGGATATAA+CGG + chr5.1:71949084-71949103 MS.gene017032:CDS 25.0%
!!! GTCACATTTTTTTAATACCA+GGG + chr5.1:71947987-71948006 MS.gene017032:intron 25.0%
AACCAGAAAAATCTAAATCC+CGG - chr5.1:71947658-71947677 None:intergenic 30.0%
AATGGATGTGATTATTGTTG+AGG + chr5.1:71947631-71947650 MS.gene017032:intron 30.0%
ACCTTTACAATATCTTGCTT+AGG - chr5.1:71947132-71947151 None:intergenic 30.0%
AGCTATATAGAAAGTAATGG+AGG - chr5.1:71948339-71948358 None:intergenic 30.0%
ATAAAGAAACAAAGGATGAG+AGG + chr5.1:71949161-71949180 MS.gene017032:CDS 30.0%
ATATTGTAAAGGTGAGATCT+CGG + chr5.1:71947139-71947158 MS.gene017032:CDS 30.0%
ATCACCAACAAGTAGTTAAT+CGG - chr5.1:71947731-71947750 None:intergenic 30.0%
ATCGCTTAAGATTTCTGATT+TGG + chr5.1:71949075-71949094 MS.gene017032:CDS 30.0%
ATTGAACAGCAATACTGTAT+TGG - chr5.1:71949143-71949162 None:intergenic 30.0%
CACCTAGAAACAAGTAAATA+TGG - chr5.1:71949540-71949559 None:intergenic 30.0%
CTACTATAATCAGAGATGTT+AGG - chr5.1:71949676-71949695 None:intergenic 30.0%
CTCCAAATTTATGAAAAAGC+TGG + chr5.1:71948929-71948948 MS.gene017032:CDS 30.0%
GAAAGAAAAATATCAAACCC+TGG - chr5.1:71948007-71948026 None:intergenic 30.0%
GACTTCAATTTGATGAATGA+GGG - chr5.1:71948171-71948190 None:intergenic 30.0%
GCATACATACTCATGTAAAT+GGG - chr5.1:71948149-71948168 None:intergenic 30.0%
GCTGTTCAATAAAGAAACAA+AGG + chr5.1:71949153-71949172 MS.gene017032:CDS 30.0%
GGTTATTGAGTTCTTATTGA+AGG - chr5.1:71948081-71948100 None:intergenic 30.0%
TACTTCACGAATTGAGATTT+GGG - chr5.1:71947948-71947967 None:intergenic 30.0%
TCCTAAGCAAGATATTGTAA+AGG + chr5.1:71947128-71947147 MS.gene017032:CDS 30.0%
TCGAGCTATATAGAAAGTAA+TGG - chr5.1:71948342-71948361 None:intergenic 30.0%
TGATTTCATTCCTTCTTTGT+AGG + chr5.1:71949736-71949755 MS.gene017032:intron 30.0%
TTACTTCACGAATTGAGATT+TGG - chr5.1:71947949-71947968 None:intergenic 30.0%
TTCTTTGAACTTCTAAATGG+GGG + chr5.1:71947003-71947022 MS.gene017032:CDS 30.0%
TTGAACAGCAATACTGTATT+GGG - chr5.1:71949142-71949161 None:intergenic 30.0%
! ATTTATGAAAAAGCTGGTTC+TGG + chr5.1:71948935-71948954 MS.gene017032:CDS 30.0%
! GCTATATAGAAAGTAATGGA+GGG - chr5.1:71948338-71948357 None:intergenic 30.0%
! GTAACTTTTCTGTCAAAATG+TGG + chr5.1:71947965-71947984 MS.gene017032:intron 30.0%
! GTTTGTTAGGATAATTGCAA+AGG + chr5.1:71946777-71946796 MS.gene017032:CDS 30.0%
! TTTGAAACGTATGAAGGTTT+CGG + chr5.1:71946612-71946631 MS.gene017032:CDS 30.0%
!! AGTAACAAGTAGAGTATTGT+CGG - chr5.1:71948039-71948058 None:intergenic 30.0%
!! TTGGTCTTCTTTTTAAAGGT+TGG + chr5.1:71948761-71948780 MS.gene017032:intron 30.0%
!! TTTTTAAAGGTTGGCGTAAA+TGG + chr5.1:71948770-71948789 MS.gene017032:intron 30.0%
!!! CTGTTTTCATGCTTTTTTTC+TGG + chr5.1:71948556-71948575 MS.gene017032:intron 30.0%
!!! GGTCACATTTTTTTAATACC+AGG + chr5.1:71947986-71948005 MS.gene017032:intron 30.0%
AAATTGTGGCATCTTGCAAA+TGG + chr5.1:71947613-71947632 MS.gene017032:intron 35.0%
AACAAGTACTTGATGGGAAT+TGG + chr5.1:71946893-71946912 MS.gene017032:CDS 35.0%
AACCCATTTCTTCTGTTTCA+TGG + chr5.1:71948828-71948847 MS.gene017032:CDS 35.0%
ACAAGTACTTGATGGGAATT+GGG + chr5.1:71946894-71946913 MS.gene017032:CDS 35.0%
ACAATATCTTGCTTAGGAGA+TGG - chr5.1:71947126-71947145 None:intergenic 35.0%
ACTCCATGAAACAGAAGAAA+TGG - chr5.1:71948834-71948853 None:intergenic 35.0%
AGTGGGAAACATATACTTTC+TGG + chr5.1:71948983-71949002 MS.gene017032:CDS 35.0%
ATAAATTTGGAGACACTTGC+CGG - chr5.1:71948921-71948940 None:intergenic 35.0%
ATACTCATGTAAATGGGCAT+GGG - chr5.1:71948143-71948162 None:intergenic 35.0%
ATGATCAAACTGCAATCATC+TGG + chr5.1:71948489-71948508 MS.gene017032:CDS 35.0%
ATGTGATTATTGTTGAGGTC+CGG + chr5.1:71947636-71947655 MS.gene017032:intron 35.0%
ATTATAAGGTCTTGCTTTGG+TGG + chr5.1:71949340-71949359 MS.gene017032:CDS 35.0%
ATTTGCATGTGGGAATTAGA+TGG + chr5.1:71949022-71949041 MS.gene017032:CDS 35.0%
CATATGTAGACCTACAAAGA+AGG - chr5.1:71949749-71949768 None:intergenic 35.0%
CATTGTCCACATTTAAGCAA+TGG - chr5.1:71949702-71949721 None:intergenic 35.0%
CGCTGTACTAAATAGCAAAA+GGG - chr5.1:71948310-71948329 None:intergenic 35.0%
CGTGTAAAGCATAGATTATC+TGG + chr5.1:71948797-71948816 MS.gene017032:CDS 35.0%
CTCCATGAAACAGAAGAAAT+GGG - chr5.1:71948833-71948852 None:intergenic 35.0%
CTTCTTTGTAGGTCTACATA+TGG + chr5.1:71949747-71949766 MS.gene017032:intron 35.0%
GAATGAACACAATCCTTTGA+AGG + chr5.1:71949430-71949449 MS.gene017032:intron 35.0%
GATCCAATGAGTTGTGAAAT+GGG - chr5.1:71947275-71947294 None:intergenic 35.0%
GCTGTGAATCTTCTTTCATT+TGG + chr5.1:71949624-71949643 MS.gene017032:intron 35.0%
GGACTTCAATTTGATGAATG+AGG - chr5.1:71948172-71948191 None:intergenic 35.0%
GGCATACATACTCATGTAAA+TGG - chr5.1:71948150-71948169 None:intergenic 35.0%
GTATACAATAGTCTCAGCAA+AGG - chr5.1:71947513-71947532 None:intergenic 35.0%
TAGTCTCCATTGCTTAAATG+TGG + chr5.1:71949693-71949712 MS.gene017032:intron 35.0%
TATTCCTTTGATGCAAAGAC+AGG - chr5.1:71947681-71947700 None:intergenic 35.0%
TCACCAACAAGTAGTTAATC+GGG - chr5.1:71947730-71947749 None:intergenic 35.0%
TCGCTGTACTAAATAGCAAA+AGG - chr5.1:71948311-71948330 None:intergenic 35.0%
TGATCAAACTGCAATCATCT+GGG + chr5.1:71948490-71948509 MS.gene017032:CDS 35.0%
TGATCCAATGAGTTGTGAAA+TGG - chr5.1:71947276-71947295 None:intergenic 35.0%
TTACGCAACAAGTACTTGAT+GGG + chr5.1:71946887-71946906 MS.gene017032:CDS 35.0%
TTCAGATCATCATTGTGGAA+AGG + chr5.1:71947308-71947327 MS.gene017032:CDS 35.0%
TTGTGATGTACTGGATTATG+TGG + chr5.1:71947585-71947604 MS.gene017032:intron 35.0%
TTTACGCAACAAGTACTTGA+TGG + chr5.1:71946886-71946905 MS.gene017032:CDS 35.0%
TTTGCATGTGGGAATTAGAT+GGG + chr5.1:71949023-71949042 MS.gene017032:CDS 35.0%
! AAACACATGATGATGAGGTA+TGG + chr5.1:71948420-71948439 MS.gene017032:CDS 35.0%
! AAACAGAAGAAATGGGTTTC+TGG - chr5.1:71948826-71948845 None:intergenic 35.0%
! CTTAGAAACACATGATGATG+AGG + chr5.1:71948415-71948434 MS.gene017032:CDS 35.0%
! GAAAGTATATGTTTCCCACT+TGG - chr5.1:71948983-71949002 None:intergenic 35.0%
! GATTAGTTGGATTGTCAAAC+GGG + chr5.1:71946641-71946660 MS.gene017032:CDS 35.0%
! GTGCACTCACATTTAGAATA+TGG + chr5.1:71948702-71948721 MS.gene017032:intron 35.0%
! TAAAGAGAGACGAGTTTGTT+AGG + chr5.1:71946764-71946783 MS.gene017032:CDS 35.0%
! TATAGAAAGTAATGGAGGGA+AGG - chr5.1:71948334-71948353 None:intergenic 35.0%
! TGATATTTTTCTTTCGCCCT+TGG + chr5.1:71948012-71948031 MS.gene017032:intron 35.0%
!! GGATTAGTTGGATTGTCAAA+CGG + chr5.1:71946640-71946659 MS.gene017032:CDS 35.0%
!! GTAACAAGTAGAGTATTGTC+GGG - chr5.1:71948038-71948057 None:intergenic 35.0%
!! TGATAAGAGCATTTGCATGT+GGG + chr5.1:71949012-71949031 MS.gene017032:CDS 35.0%
!! TTTTGTGCATTGATGAGTGA+GGG - chr5.1:71948659-71948678 None:intergenic 35.0%
!! TTTTTGTGCATTGATGAGTG+AGG - chr5.1:71948660-71948679 None:intergenic 35.0%
!!! TTTTCAGGTATCATCACTGT+TGG - chr5.1:71947201-71947220 None:intergenic 35.0%
AAAAATCATCCCCAAAAGCC+AGG + chr5.1:71948238-71948257 MS.gene017032:intron 40.0%
AAAATCATCCCCAAAAGCCA+GGG + chr5.1:71948239-71948258 MS.gene017032:intron 40.0%
AAAGAAGTGGAGTCTTGGAA+AGG + chr5.1:71949046-71949065 MS.gene017032:CDS 40.0%
AACAAGTAGTTAATCGGGGT+TGG - chr5.1:71947725-71947744 None:intergenic 40.0%
AACCCCGATTAACTACTTGT+TGG + chr5.1:71947724-71947743 MS.gene017032:intron 40.0%
AAGTTCCTCCATTGCAGTAA+TGG - chr5.1:71949952-71949971 None:intergenic 40.0%
AAGTTTCTCAAGTGACTCCA+TGG + chr5.1:71946687-71946706 MS.gene017032:CDS 40.0%
AATGGCATCCATTACTGCAA+TGG + chr5.1:71949941-71949960 MS.gene017032:CDS 40.0%
ACCTTCATACGTTTCAAAGC+TGG - chr5.1:71946610-71946629 None:intergenic 40.0%
AGATCTGTCACAGTATTAGC+AGG - chr5.1:71948397-71948416 None:intergenic 40.0%
AGATGACACTATTGTCAGGT+CGG + chr5.1:71946954-71946973 MS.gene017032:CDS 40.0%
ATGACCGTACAATTCGGATA+TGG + chr5.1:71949876-71949895 MS.gene017032:CDS 40.0%
ATGGCTTAACTCTGCAACAT+TGG + chr5.1:71948721-71948740 MS.gene017032:intron 40.0%
ATTTGGATATAACGGCTGAC+GGG + chr5.1:71949092-71949111 MS.gene017032:CDS 40.0%
CACCAACAAGTAGTTAATCG+GGG - chr5.1:71947729-71947748 None:intergenic 40.0%
CAGGGATAGTAGTAGAATTC+GGG + chr5.1:71947077-71947096 MS.gene017032:CDS 40.0%
CAGTGATGATACCTGAAAAG+AGG + chr5.1:71947202-71947221 MS.gene017032:CDS 40.0%
CATACTCATGTAAATGGGCA+TGG - chr5.1:71948144-71948163 None:intergenic 40.0%
CGCTTGGTTATAGAAAGAGT+GGG + chr5.1:71946809-71946828 MS.gene017032:CDS 40.0%
CTGTTCCAATATCAAACACG+CGG - chr5.1:71946980-71946999 None:intergenic 40.0%
GATGATACCTGAAAAGAGGT+TGG + chr5.1:71947206-71947225 MS.gene017032:CDS 40.0%
GATTTGGATATAACGGCTGA+CGG + chr5.1:71949091-71949110 MS.gene017032:CDS 40.0%
GCAGGGATAGTAGTAGAATT+CGG + chr5.1:71947076-71947095 MS.gene017032:CDS 40.0%
GCTTGGTTATAGAAAGAGTG+GGG + chr5.1:71946810-71946829 MS.gene017032:CDS 40.0%
GGAATTAGATGGGAAAGAAG+TGG + chr5.1:71949033-71949052 MS.gene017032:CDS 40.0%
GTAGCTACATTGCGTAAGAT+TGG + chr5.1:71946925-71946944 MS.gene017032:CDS 40.0%
GTCTCTCTTTATAACCCCTT+TGG - chr5.1:71946755-71946774 None:intergenic 40.0%
TCACTTGAGAAACTTCCAAC+AGG - chr5.1:71946682-71946701 None:intergenic 40.0%
TCGCTTGGTTATAGAAAGAG+TGG + chr5.1:71946808-71946827 MS.gene017032:CDS 40.0%
TCTAAATGGGGGAAAGGTTA+TGG + chr5.1:71947014-71947033 MS.gene017032:CDS 40.0%
TGAAAAGAGGTTGGAACATC+TGG + chr5.1:71947215-71947234 MS.gene017032:CDS 40.0%
TGAACTTCTAAATGGGGGAA+AGG + chr5.1:71947008-71947027 MS.gene017032:CDS 40.0%
TGACAGATGACACTATTGTC+AGG + chr5.1:71946950-71946969 MS.gene017032:CDS 40.0%
TGACCGTACAATTCGGATAT+GGG + chr5.1:71949877-71949896 MS.gene017032:CDS 40.0%
TGGCTTAACTCTGCAACATT+GGG + chr5.1:71948722-71948741 MS.gene017032:intron 40.0%
TGTGATTATTGTTGAGGTCC+GGG + chr5.1:71947637-71947656 MS.gene017032:intron 40.0%
TTCTAGGTGTGATGTTCAGT+AGG + chr5.1:71949551-71949570 MS.gene017032:intron 40.0%
TTGCCCATTTCACAACTCAT+TGG + chr5.1:71947269-71947288 MS.gene017032:CDS 40.0%
TTGTACTTCCCAATGAGCTT+AGG - chr5.1:71949298-71949317 None:intergenic 40.0%
! AAGCTCATTGGGAAGTACAA+AGG + chr5.1:71949298-71949317 MS.gene017032:CDS 40.0%
! AGATGTTCCAACCTCTTTTC+AGG - chr5.1:71947216-71947235 None:intergenic 40.0%
! ATCAGAGATGTTAGGTACTG+CGG - chr5.1:71949668-71949687 None:intergenic 40.0%
! CTAACCTGTACTGTTCTAGA+TGG - chr5.1:71947342-71947361 None:intergenic 40.0%
! GATACCATCTAGAACAGTAC+AGG + chr5.1:71947335-71947354 MS.gene017032:CDS 40.0%
! GTCCGGGATTTAGATTTTTC+TGG + chr5.1:71947653-71947672 MS.gene017032:intron 40.0%
! TCTTCTTCTAAAGAGCCTGT+TGG + chr5.1:71946664-71946683 MS.gene017032:CDS 40.0%
! TTGGGAAGTACAAAGGTCAT+AGG + chr5.1:71949305-71949324 MS.gene017032:CDS 40.0%
!! ACCAGCTTTGAAACGTATGA+AGG + chr5.1:71946606-71946625 MS.gene017032:CDS 40.0%
!! GCTGACTTGTTGATCAGTAA+CGG + chr5.1:71947467-71947486 MS.gene017032:intron 40.0%
!! GTGATAAGAGCATTTGCATG+TGG + chr5.1:71949011-71949030 MS.gene017032:CDS 40.0%
AAAGGTTCCAGTCTACAAGG+CGG - chr5.1:71947495-71947514 None:intergenic 45.0%
AAGCCTCTAACTGCAAGGTA+AGG - chr5.1:71947251-71947270 None:intergenic 45.0%
AAGGCGATCCTAAGCTCATT+GGG + chr5.1:71949287-71949306 MS.gene017032:CDS 45.0%
AAGTGGAAGTGAGGATTCAC+AGG + chr5.1:71949384-71949403 MS.gene017032:CDS 45.0%
AGCAAAGGTTCCAGTCTACA+AGG - chr5.1:71947498-71947517 None:intergenic 45.0%
AGCCTCTAACTGCAAGGTAA+GGG - chr5.1:71947250-71947269 None:intergenic 45.0%
AGGAGATTACTCCACTTTGC+AGG + chr5.1:71947058-71947077 MS.gene017032:CDS 45.0%
ATCTCGGTCAAAGCTTCTAG+AGG + chr5.1:71947155-71947174 MS.gene017032:CDS 45.0%
ATGGGAAAGAAGTGGAGTCT+TGG + chr5.1:71949041-71949060 MS.gene017032:CDS 45.0%
CCAGTGATGACCGTACAATT+CGG + chr5.1:71949870-71949889 MS.gene017032:CDS 45.0%
CCTGTACATGGTTTCCAAGT+GGG + chr5.1:71948966-71948985 MS.gene017032:CDS 45.0%
CTACTACTATCCCTGCAAAG+TGG - chr5.1:71947072-71947091 None:intergenic 45.0%
GAAGTGAGGATTCACAGGTA+TGG + chr5.1:71949389-71949408 MS.gene017032:intron 45.0%
GACCGTACAATTCGGATATG+GGG + chr5.1:71949878-71949897 MS.gene017032:CDS 45.0%
GAGCTGTTAATTGTGTGAGC+TGG + chr5.1:71949816-71949835 MS.gene017032:CDS 45.0%
GGAGATTACTCCACTTTGCA+GGG + chr5.1:71947059-71947078 MS.gene017032:CDS 45.0%
GGAGGAATCTATTAGGCGTT+GGG + chr5.1:71948886-71948905 MS.gene017032:CDS 45.0%
GGCATTTGGCTGTGATTTGA+AGG - chr5.1:71948638-71948657 None:intergenic 45.0%
GGCGCTCGAGGTTTATTATA+AGG + chr5.1:71949326-71949345 MS.gene017032:CDS 45.0%
GGGAGGAGAAAATCGAGAAA+AGG + chr5.1:71948259-71948278 MS.gene017032:intron 45.0%
GGTTCCTGTCTTTGCATCAA+AGG + chr5.1:71947674-71947693 MS.gene017032:intron 45.0%
GTGGAGTGGAGGAATCTATT+AGG + chr5.1:71948879-71948898 MS.gene017032:CDS 45.0%
TCAAACTGCAATCATCTGGG+AGG + chr5.1:71948493-71948512 MS.gene017032:CDS 45.0%
TCAGGTATCATCACTGTTGG+AGG - chr5.1:71947198-71947217 None:intergenic 45.0%
TCCTGTACATGGTTTCCAAG+TGG + chr5.1:71948965-71948984 MS.gene017032:CDS 45.0%
TGCGTAAACAGATTGACACC+AGG - chr5.1:71946874-71946893 None:intergenic 45.0%
TGGAGGAATCTATTAGGCGT+TGG + chr5.1:71948885-71948904 MS.gene017032:CDS 45.0%
TTAACAGCTCCTGAATGACC+AGG - chr5.1:71949806-71949825 None:intergenic 45.0%
TTTCGTTGCAAGTGGAAGTG+AGG + chr5.1:71949375-71949394 MS.gene017032:CDS 45.0%
TTTGGATATAACGGCTGACG+GGG + chr5.1:71949093-71949112 MS.gene017032:CDS 45.0%
! AGATGTTAGGTACTGCGGTA+TGG - chr5.1:71949663-71949682 None:intergenic 45.0%
! GTTTCGGCGAAAGGATTAGT+TGG + chr5.1:71946628-71946647 MS.gene017032:CDS 45.0%
! TTTCTCGATTTTCTCCTCCC+TGG - chr5.1:71948259-71948278 None:intergenic 45.0%
!! ATCAGGTTGTCGGTTCCAAA+GGG + chr5.1:71946737-71946756 MS.gene017032:CDS 45.0%
!! ATTTTCTCCTCCCTGGCTTT+TGG - chr5.1:71948252-71948271 None:intergenic 45.0%
!! CATTGATGAGTGAGGGCATT+TGG - chr5.1:71948652-71948671 None:intergenic 45.0%
!! GCAAAGGCATTGTATTCGCT+TGG + chr5.1:71946793-71946812 MS.gene017032:CDS 45.0%
!! GGAAGCATTGAAGACCTTGA+GGG + chr5.1:71947035-71947054 MS.gene017032:CDS 45.0%
!! TGGAAGCATTGAAGACCTTG+AGG + chr5.1:71947034-71947053 MS.gene017032:CDS 45.0%
!! TTTTCTCCTCCCTGGCTTTT+GGG - chr5.1:71948251-71948270 None:intergenic 45.0%
ACTGGCAGAGGCTAACATGT+GGG - chr5.1:71949855-71949874 None:intergenic 50.0%
AGCCCTTACCTTGCAGTTAG+AGG + chr5.1:71947245-71947264 MS.gene017032:CDS 50.0%
AGGAGATGGAGACACTAAGC+AGG - chr5.1:71947112-71947131 None:intergenic 50.0%
ATTCCTCCACTCCACATGTG+AGG - chr5.1:71948874-71948893 None:intergenic 50.0%
CCCACTTGGAAACCATGTAC+AGG - chr5.1:71948969-71948988 None:intergenic 50.0%
CCGAATTGTACGGTCATCAC+TGG - chr5.1:71949873-71949892 None:intergenic 50.0%
CGTATGAAGGTTTCGGCGAA+AGG + chr5.1:71946619-71946638 MS.gene017032:CDS 50.0%
CTGGCTTAGTCTCCTGTACA+TGG + chr5.1:71948954-71948973 MS.gene017032:CDS 50.0%
GAAGGCGATCCTAAGCTCAT+TGG + chr5.1:71949286-71949305 MS.gene017032:CDS 50.0%
GAGCAAGCTTTCGTTGCAAG+TGG + chr5.1:71949367-71949386 MS.gene017032:CDS 50.0%
GGAATACCTTTGCACTCACC+TGG + chr5.1:71946853-71946872 MS.gene017032:CDS 50.0%
GGCATCCATTACTGCAATGG+AGG + chr5.1:71949944-71949963 MS.gene017032:CDS 50.0%
GGCATGGGTTTGCATCGAAA+AGG - chr5.1:71948128-71948147 None:intergenic 50.0%
GGCCCCATATCCGAATTGTA+CGG - chr5.1:71949883-71949902 None:intergenic 50.0%
GTGAGGAGCTCTTCGTCATT+AGG - chr5.1:71948857-71948876 None:intergenic 50.0%
GTTATAGAAAGAGTGGGGCG+CGG + chr5.1:71946815-71946834 MS.gene017032:CDS 50.0%
TACCAACATGCGCACAGCAA+TGG + chr5.1:71949923-71949942 MS.gene017032:CDS 50.0%
TCTCAAGTGACTCCATGGCT+CGG + chr5.1:71946692-71946711 MS.gene017032:CDS 50.0%
TGGAGTAATCTCCTCCCTCA+AGG - chr5.1:71947052-71947071 None:intergenic 50.0%
TTGACACCAGGTGAGTGCAA+AGG - chr5.1:71946862-71946881 None:intergenic 50.0%
! AGCATTGAAGACCTTGAGGG+AGG + chr5.1:71947038-71947057 MS.gene017032:CDS 50.0%
! GCTAACATGTGGGGATTAGC+TGG - chr5.1:71949845-71949864 None:intergenic 50.0%
! TACATATGGCACCGAAGCTC+AGG + chr5.1:71949761-71949780 MS.gene017032:CDS 50.0%
!! AGGCGTTGGGATGTTTCTAC+CGG + chr5.1:71948899-71948918 MS.gene017032:CDS 50.0%
!! AGTAGAGTATTGTCGGGCCA+AGG - chr5.1:71948032-71948051 None:intergenic 50.0%
!! GATCAGGTTGTCGGTTCCAA+AGG + chr5.1:71946736-71946755 MS.gene017032:CDS 50.0%
!! GTAGAGTATTGTCGGGCCAA+GGG - chr5.1:71948031-71948050 None:intergenic 50.0%
!! TAGATAGGAGCACTGACTCG+TGG - chr5.1:71948193-71948212 None:intergenic 50.0%
!! TCAGGTTGTCGGTTCCAAAG+GGG + chr5.1:71946738-71946757 MS.gene017032:CDS 50.0%
!! TTTCTCCTCCCTGGCTTTTG+GGG - chr5.1:71948250-71948269 None:intergenic 50.0%
AAGCTCAGGAGAGCCGATAG+AGG + chr5.1:71949775-71949794 MS.gene017032:CDS 55.0%
ACAAAGGTCATAGGCGCTCG+AGG + chr5.1:71949314-71949333 MS.gene017032:CDS 55.0%
AGAGCTCCTCACATGTGGAG+TGG + chr5.1:71948865-71948884 MS.gene017032:CDS 55.0%
ATCATCCCCAAAAGCCAGGG+AGG + chr5.1:71948242-71948261 MS.gene017032:intron 55.0%
CACTGGCAGAGGCTAACATG+TGG - chr5.1:71949856-71949875 None:intergenic 55.0%
CTGGCAGAGGCTAACATGTG+GGG - chr5.1:71949854-71949873 None:intergenic 55.0%
GACAACCTGATCGTCCCCTT+CGG - chr5.1:71946728-71946747 None:intergenic 55.0%
GACGAAGAGCTCCTCACATG+TGG + chr5.1:71948860-71948879 MS.gene017032:CDS 55.0%
GTCGGCCGCGTGTTTGATAT+TGG + chr5.1:71946972-71946991 MS.gene017032:CDS 55.0%
TGACCAGGCAGTGCCTCTAT+CGG - chr5.1:71949791-71949810 None:intergenic 55.0%
TGCCATTGCTGTGCGCATGT+TGG - chr5.1:71949928-71949947 None:intergenic 55.0%
TGGGCAAGCCTCTAACTGCA+AGG - chr5.1:71947256-71947275 None:intergenic 55.0%
TGTACGGTCATCACTGGCAG+AGG - chr5.1:71949867-71949886 None:intergenic 55.0%
! GAAGGGGACGATCAGGTTGT+CGG + chr5.1:71946727-71946746 MS.gene017032:CDS 55.0%
!! CTATCGGCTCTCCTGAGCTT+CGG - chr5.1:71949775-71949794 None:intergenic 55.0%
AACGGCACCGCCTTGTAGAC+TGG + chr5.1:71947485-71947504 MS.gene017032:intron 60.0%
ACCTGATCGTCCCCTTCGGA+CGG - chr5.1:71946724-71946743 None:intergenic 60.0%
CTCGGCTACTACCGTCCGAA+GGG + chr5.1:71946710-71946729 MS.gene017032:CDS 60.0%
GCTCCTCACATGTGGAGTGG+AGG + chr5.1:71948868-71948887 MS.gene017032:CDS 60.0%
TCGGCTACTACCGTCCGAAG+GGG + chr5.1:71946711-71946730 MS.gene017032:CDS 60.0%
!! GAGGCACTGCCTGGTCATTC+AGG + chr5.1:71949794-71949813 MS.gene017032:CDS 60.0%
AAAGAGTGGGGCGCGGCTAG+AGG + chr5.1:71946822-71946841 MS.gene017032:CDS 65.0%
ACCGTCCGAAGGGGACGATC+AGG + chr5.1:71946720-71946739 MS.gene017032:CDS 65.0%
GCTCGGCTACTACCGTCCGA+AGG + chr5.1:71946709-71946728 MS.gene017032:CDS 65.0%
GGACGGTAGTAGCCGAGCCA+TGG - chr5.1:71946707-71946726 None:intergenic 65.0%
!! GAGCCGATAGAGGCACTGCC+TGG + chr5.1:71949785-71949804 MS.gene017032:CDS 65.0%
GCGCGGCTAGAGGAGGAGTC+TGG + chr5.1:71946832-71946851 MS.gene017032:CDS 70.0%
GAGTGGGGCGCGGCTAGAGG+AGG + chr5.1:71946825-71946844 MS.gene017032:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 71946583 71949973 71946583 ID=MS.gene017032
chr5.1 mRNA 71946583 71949973 71946583 ID=MS.gene017032.t1;Parent=MS.gene017032
chr5.1 exon 71946583 71947356 71946583 ID=MS.gene017032.t1.exon1;Parent=MS.gene017032.t1
chr5.1 CDS 71946583 71947356 71946583 ID=cds.MS.gene017032.t1;Parent=MS.gene017032.t1
chr5.1 exon 71948413 71948514 71948413 ID=MS.gene017032.t1.exon2;Parent=MS.gene017032.t1
chr5.1 CDS 71948413 71948514 71948413 ID=cds.MS.gene017032.t1;Parent=MS.gene017032.t1
chr5.1 exon 71948779 71949405 71948779 ID=MS.gene017032.t1.exon3;Parent=MS.gene017032.t1
chr5.1 CDS 71948779 71949405 71948779 ID=cds.MS.gene017032.t1;Parent=MS.gene017032.t1
chr5.1 exon 71949758 71949973 71949758 ID=MS.gene017032.t1.exon4;Parent=MS.gene017032.t1
chr5.1 CDS 71949758 71949973 71949758 ID=cds.MS.gene017032.t1;Parent=MS.gene017032.t1
Gene Sequence

>MS.gene017032

ATGGGAGGTGTGGACGATGAAGAACCAGCTTTGAAACGTATGAAGGTTTCGGCGAAAGGATTAGTTGGATTGTCAAACGGGTCTTCTTCTAAAGAGCCTGTTGGAAGTTTCTCAAGTGACTCCATGGCTCGGCTACTACCGTCCGAAGGGGACGATCAGGTTGTCGGTTCCAAAGGGGTTATAAAGAGAGACGAGTTTGTTAGGATAATTGCAAAGGCATTGTATTCGCTTGGTTATAGAAAGAGTGGGGCGCGGCTAGAGGAGGAGTCTGGAATACCTTTGCACTCACCTGGTGTCAATCTGTTTACGCAACAAGTACTTGATGGGAATTGGGATGAGAGTGTAGCTACATTGCGTAAGATTGGTTTGACAGATGACACTATTGTCAGGTCGGCCGCGTGTTTGATATTGGAACAGAAATTCTTTGAACTTCTAAATGGGGGAAAGGTTATGGAAGCATTGAAGACCTTGAGGGAGGAGATTACTCCACTTTGCAGGGATAGTAGTAGAATTCGGGAACTTTCGTCCTGCTTAGTGTCTCCATCTCCTAAGCAAGATATTGTAAAGGTGAGATCTCGGTCAAAGCTTCTAGAGGAATTGCAGAAACTGCTTCCTCCAACAGTGATGATACCTGAAAAGAGGTTGGAACATCTGGTTGAACAAGCCCTTACCTTGCAGTTAGAGGCTTGCCCATTTCACAACTCATTGGATCAGATGTCGTTATATTCAGATCATCATTGTGGAAAGGATCTGATACCATCTAGAACAGTACAGGTTAGAAAATTATATGTCTAAATTCTAATAGATGCTGTTAGCTTTAAATGTGAGTTGTTTGACTTCTATTCTTCTTAATACATGTTAAGAAATTGCGATATTTATTGATGGCTGACTTGTTGATCAGTAACGGCACCGCCTTGTAGACTGGAACCTTTGCTGAGACTATTGTATACTTTTGCATCATTATTATTATCATGTTTGTTTTTTCTTTACTATAAAATTATGTTGTGATGTACTGGATTATGTGGATAATAAATTGTGGCATCTTGCAAATGGATGTGATTATTGTTGAGGTCCGGGATTTAGATTTTTCTGGTTCCTGTCTTTGCATCAAAGGAATATTAATGATGAATAAGATTTGACCAACCCCGATTAACTACTTGTTGGTGATATCCAATTATGATAGATTTTGGAAAAGACTTGATAACTACATGCTAGTTTGTCATCTGAGTTCACTAAATTAATGGTGATATAACTAGACAAAATCTTCTATTGGATGAAATGATAGTCTATGTGCATGTACTTACTATCTCATGAAAGATTAGTTGAAATTTTCTCTAGTGACAGTTGCATCTATTTTGCTTTCCCAAATCTCAATTCGTGAAGTAACTTTTCTGTCAAAATGTGGTCACATTTTTTTAATACCAGGGTTTGATATTTTTCTTTCGCCCTTGGCCCGACAATACTCTACTTGTTACTGTTTGAGAGTGTTGAGTTTCCTTCAATAAGAACTCAATAACCTTATAGTAAAAATTCTATAAAATGCCTTTTCGATGCAAACCCATGCCCATTTACATGAGTATGTATGCCCTCATTCATCAAATTGAAGTCCACGAGTCAGTGCTCCTATCTATATTATTTTTAATCTGATTTCTTTGAAAAATCATCCCCAAAAGCCAGGGAGGAGAAAATCGAGAAAAGGAGTATAATTTTGTCTTTCTGTTTAACCCTTTTGCTATTTAGTACAGCGACCTTCCCTCCATTACTTTCTATATAGCTCGAGTGTTATCTATATTCTGATGATATTTTCTTTTCCTGCTAATACTGTGACAGATCTTAGAAACACATGATGATGAGGTATGGTTTGTACAATTTTCACATAATGGGAAGTATTTAGCTTCAGCGTCAAATGATCAAACTGCAATCATCTGGGAGGTAATATTTGATTTTGATCTGAGAATTTCAATTTGTTCTCTCTGTTTTCATGCTTTTTTTCTGGTTTTATGTGTCAAATGTTTTTTAAGTTTCAATTTGAAATCCTAGTTGTAATTTTTTCCTTCAAATCACAGCCAAATGCCCTCACTCATCAATGCACAAAAATTGCTCACTCATACATGCACGCGTGCACTCACATTTAGAATATGGCTTAACTCTGCAACATTGGGTTTTAGATTTTAATTTTTGGTCTTCTTTTTAAAGGTTGGCGTAAATGGAGTGCGTGTAAAGCATAGATTATCTGGTCACCAGAAACCCATTTCTTCTGTTTCATGGAGTCCTAATGACGAAGAGCTCCTCACATGTGGAGTGGAGGAATCTATTAGGCGTTGGGATGTTTCTACCGGCAAGTGTCTCCAAATTTATGAAAAAGCTGGTTCTGGCTTAGTCTCCTGTACATGGTTTCCAAGTGGGAAACATATACTTTCTGGCTTAAGTGATAAGAGCATTTGCATGTGGGAATTAGATGGGAAAGAAGTGGAGTCTTGGAAAGGACAAAAATCGCTTAAGATTTCTGATTTGGATATAACGGCTGACGGGGAAGAGATTATAAGTATCTGTAAACCCAATACAGTATTGCTGTTCAATAAAGAAACAAAGGATGAGAGGTTTATTGAAGAGCGCCAAACAATAACTTCTTTTTCTTTGTCAAAGGATAATAAATTTTTGTTGGTTAATCTTTTGAACCAAGAAATTAATCTTTGGAATATTGAAGGCGATCCTAAGCTCATTGGGAAGTACAAAGGTCATAGGCGCTCGAGGTTTATTATAAGGTCTTGCTTTGGTGGACTTGAGCAAGCTTTCGTTGCAAGTGGAAGTGAGGATTCACAGGTATGGATCATTATTCATTGATAAGAATGAACACAATCCTTTGAAGGATAATATCTTGTAATTTGCAAAATAAAATTCTTTTGGTGCTTGTATGTTTATCACTTTATGTCTTACAAGGGCCTTTAGAAAATCCATATTTACTTGTTTCTAGGTGTGATGTTCAGTAGGTTTCCTGAGATTAGATATAAAATGTAGTTTGGATTAGTAGAAATTTAGCAGCTGTGAATCTTCTTTCATTTGGATGTAGAACAATTCCATACCGCAGTACCTAACATCTCTGATTATAGTAGTCTCCATTGCTTAAATGTGGACAATGATTAAACTTGAGTTTGATTTCATTCCTTCTTTGTAGGTCTACATATGGCACCGAAGCTCAGGAGAGCCGATAGAGGCACTGCCTGGTCATTCAGGAGCTGTTAATTGTGTGAGCTGGAATCCAGCTAATCCCCACATGTTAGCCTCTGCCAGTGATGACCGTACAATTCGGATATGGGGCCTTAATAGTCTGAATAAAAAGTACCAACATGCGCACAGCAATGGCATCCATTACTGCAATGGAGGAACTTAG
Protein sequence

>MS.gene017032.t1

MGGVDDEEPALKRMKVSAKGLVGLSNGSSSKEPVGSFSSDSMARLLPSEGDDQVVGSKGVIKRDEFVRIIAKALYSLGYRKSGARLEEESGIPLHSPGVNLFTQQVLDGNWDESVATLRKIGLTDDTIVRSAACLILEQKFFELLNGGKVMEALKTLREEITPLCRDSSRIRELSSCLVSPSPKQDIVKVRSRSKLLEELQKLLPPTVMIPEKRLEHLVEQALTLQLEACPFHNSLDQMSLYSDHHCGKDLIPSRTVQILETHDDEVWFVQFSHNGKYLASASNDQTAIIWEVGVNGVRVKHRLSGHQKPISSVSWSPNDEELLTCGVEESIRRWDVSTGKCLQIYEKAGSGLVSCTWFPSGKHILSGLSDKSICMWELDGKEVESWKGQKSLKISDLDITADGEEIISICKPNTVLLFNKETKDERFIEERQTITSFSLSKDNKFLLVNLLNQEINLWNIEGDPKLIGKYKGHRRSRFIIRSCFGGLEQAFVASGSEDSQVYIWHRSSGEPIEALPGHSGAVNCVSWNPANPHMLASASDDRTIRIWGLNSLNKKYQHAHSNGIHYCNGGT