Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042091.t1 | PNY14930.1 | 95.2 | 124 | 6 | 0 | 1 | 124 | 1 | 124 | 5.10E-59 | 236.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042091.t1 | Q9FGJ4 | 56.7 | 127 | 51 | 1 | 1 | 123 | 1 | 127 | 2.5e-30 | 132.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042091.t1 | A0A2K3PI09 | 95.2 | 124 | 6 | 0 | 1 | 124 | 1 | 124 | 3.7e-59 | 236.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene72802 | MS.gene042091 | PPI |
| MS.gene033495 | MS.gene042091 | PPI |
| MS.gene042091 | MS.gene033497 | PPI |
| MS.gene064261 | MS.gene042091 | PPI |
| MS.gene042091 | MS.gene058580 | PPI |
| MS.gene042091 | MS.gene024056 | PPI |
| MS.gene042091 | MS.gene88356 | PPI |
| MS.gene82126 | MS.gene042091 | PPI |
| MS.gene52597 | MS.gene042091 | PPI |
| MS.gene028119 | MS.gene042091 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042091.t1 | MTR_4g133928 | 94.355 | 124 | 7 | 0 | 1 | 124 | 1 | 124 | 8.31e-83 | 238 |
| MS.gene042091.t1 | MTR_0446s0010 | 88.889 | 108 | 9 | 1 | 1 | 108 | 1 | 105 | 9.34e-65 | 192 |
| MS.gene042091.t1 | MTR_7g080390 | 50.649 | 77 | 38 | 0 | 47 | 123 | 54 | 130 | 2.57e-21 | 82.8 |
| MS.gene042091.t1 | MTR_7g080760 | 48.649 | 74 | 38 | 0 | 47 | 120 | 43 | 116 | 2.59e-21 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042091.t1 | AT5G47630 | 56.693 | 127 | 51 | 1 | 1 | 123 | 1 | 127 | 1.04e-42 | 137 |
| MS.gene042091.t1 | AT5G47630 | 56.693 | 127 | 51 | 1 | 1 | 123 | 1 | 127 | 1.04e-42 | 137 |
| MS.gene042091.t1 | AT5G47630 | 56.693 | 127 | 51 | 1 | 1 | 123 | 1 | 127 | 1.04e-42 | 137 |
| MS.gene042091.t1 | AT1G65290 | 50.649 | 77 | 38 | 0 | 47 | 123 | 50 | 126 | 3.40e-21 | 82.4 |
| MS.gene042091.t1 | AT2G44620 | 41.000 | 100 | 46 | 1 | 17 | 116 | 29 | 115 | 6.13e-21 | 81.6 |
Find 18 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATAGGGTTGAGCTTATTA+TGG | 0.307345 | 8.3:-604816 | MS.gene042091:CDS |
| AGCTTATCTGCTTTCTCATC+AGG | 0.361227 | 8.3:+604764 | None:intergenic |
| CATAATCACATTATCATCCT+TGG | 0.439969 | 8.3:+605110 | None:intergenic |
| GGACTTAAACCTGGACAGCT+TGG | 0.459469 | 8.3:-604837 | MS.gene042091:CDS |
| TGATTATGCATTTGAGATGT+TGG | 0.460487 | 8.3:-605095 | MS.gene042091:CDS |
| TGAGAAGGTCAACTGAAAGA+TGG | 0.475010 | 8.3:-605140 | MS.gene042091:CDS |
| GTCTAGTATCGGATCAGAGC+TGG | 0.475971 | 8.3:+605056 | None:intergenic |
| AGATGTTGGTGCTCTTCAGC+AGG | 0.487417 | 8.3:-605081 | MS.gene042091:CDS |
| CAACCCTATCCAAGCTGTCC+AGG | 0.498453 | 8.3:+604828 | None:intergenic |
| TAAACCTGGACAGCTTGGAT+AGG | 0.517739 | 8.3:-604832 | MS.gene042091:CDS |
| TCTTGACCAGTCTGATTACT+CGG | 0.521850 | 8.3:+605034 | None:intergenic |
| AAACCTGGACAGCTTGGATA+GGG | 0.522123 | 8.3:-604831 | MS.gene042091:CDS |
| CTGATTACTCGGTCTAGTAT+CGG | 0.536163 | 8.3:+605045 | None:intergenic |
| GGCTATGTACTTTGCAACAT+CGG | 0.551029 | 8.3:+604730 | None:intergenic |
| TTTCTCATCAGGAATTTCGA+TGG | 0.580152 | 8.3:+604775 | None:intergenic |
| TGATAAAATTAATGCAGCAA+AGG | 0.580568 | 8.3:-605007 | MS.gene042091:intron |
| TAAGTCATGTGAATTTGAGA+AGG | 0.613186 | 8.3:-605155 | MS.gene042091:CDS |
| ACTAGACCGAGTAATCAGAC+TGG | 0.680991 | 8.3:-605040 | MS.gene042091:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGGTATATATTTTTGTGT+AGG | - | chr8.3:605001-605020 | MS.gene042091:intron | 20.0% |
| !!! | TATTTTCTTTTGACTATAAC+TGG | + | chr8.3:604909-604928 | None:intergenic | 20.0% |
| ! | TGATAAAATTAATGCAGCAA+AGG | - | chr8.3:604879-604898 | MS.gene042091:intron | 25.0% |
| CATAATCACATTATCATCCT+TGG | + | chr8.3:604779-604798 | None:intergenic | 30.0% | |
| TAAGTCATGTGAATTTGAGA+AGG | - | chr8.3:604731-604750 | MS.gene042091:CDS | 30.0% | |
| TTCACGTGTAGATTAAGTAT+TGG | - | chr8.3:604983-605002 | MS.gene042091:intron | 30.0% | |
| ! | TGATTATGCATTTGAGATGT+TGG | - | chr8.3:604791-604810 | MS.gene042091:CDS | 30.0% |
| ! | TTTTCAAAAGGACTTAAACC+TGG | - | chr8.3:605040-605059 | MS.gene042091:CDS | 30.0% |
| TTATCAGTGTTACACAGTCT+GGG | - | chr8.3:604952-604971 | MS.gene042091:intron | 35.0% | |
| TTTATCAGTGTTACACAGTC+TGG | - | chr8.3:604951-604970 | MS.gene042091:intron | 35.0% | |
| TTTCTCATCAGGAATTTCGA+TGG | + | chr8.3:605114-605133 | None:intergenic | 35.0% | |
| ! | GTCAACTGAAAGATGGTTTT+TGG | - | chr8.3:604753-604772 | MS.gene042091:CDS | 35.0% |
| !! | TGAAGCAGCTGATTTTCAAA+AGG | - | chr8.3:605028-605047 | MS.gene042091:CDS | 35.0% |
| !!! | AATTTTTTGGTCAGCTTCAG+AGG | + | chr8.3:605180-605199 | None:intergenic | 35.0% |
| AGCTTATCTGCTTTCTCATC+AGG | + | chr8.3:605125-605144 | None:intergenic | 40.0% | |
| CTGATTACTCGGTCTAGTAT+CGG | + | chr8.3:604844-604863 | None:intergenic | 40.0% | |
| GGATAGGGTTGAGCTTATTA+TGG | - | chr8.3:605070-605089 | MS.gene042091:CDS | 40.0% | |
| GGCTATGTACTTTGCAACAT+CGG | + | chr8.3:605159-605178 | None:intergenic | 40.0% | |
| TCTTGACCAGTCTGATTACT+CGG | + | chr8.3:604855-604874 | None:intergenic | 40.0% | |
| TGAGAAGGTCAACTGAAAGA+TGG | - | chr8.3:604746-604765 | MS.gene042091:CDS | 40.0% | |
| !!! | TGAAAGATGGTTTTTGGCCA+AGG | - | chr8.3:604759-604778 | MS.gene042091:CDS | 40.0% |
| AAACCTGGACAGCTTGGATA+GGG | - | chr8.3:605055-605074 | MS.gene042091:CDS | 45.0% | |
| ACTAGACCGAGTAATCAGAC+TGG | - | chr8.3:604846-604865 | MS.gene042091:CDS | 45.0% | |
| TAAACCTGGACAGCTTGGAT+AGG | - | chr8.3:605054-605073 | MS.gene042091:CDS | 45.0% | |
| GGACTTAAACCTGGACAGCT+TGG | - | chr8.3:605049-605068 | MS.gene042091:CDS | 50.0% | |
| ! | AGATGTTGGTGCTCTTCAGC+AGG | - | chr8.3:604805-604824 | MS.gene042091:CDS | 50.0% |
| ! | GTCTAGTATCGGATCAGAGC+TGG | + | chr8.3:604833-604852 | None:intergenic | 50.0% |
| CAACCCTATCCAAGCTGTCC+AGG | + | chr8.3:605061-605080 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 604706 | 605202 | 604706 | ID=MS.gene042091 |
| chr8.3 | mRNA | 604706 | 605202 | 604706 | ID=MS.gene042091.t1;Parent=MS.gene042091 |
| chr8.3 | exon | 605008 | 605202 | 605008 | ID=MS.gene042091.t1.exon1;Parent=MS.gene042091.t1 |
| chr8.3 | CDS | 605008 | 605202 | 605008 | ID=cds.MS.gene042091.t1;Parent=MS.gene042091.t1 |
| chr8.3 | exon | 604706 | 604885 | 604706 | ID=MS.gene042091.t1.exon2;Parent=MS.gene042091.t1 |
| chr8.3 | CDS | 604706 | 604885 | 604706 | ID=cds.MS.gene042091.t1;Parent=MS.gene042091.t1 |
| Gene Sequence |
| Protein sequence |
