Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042866.t1 | XP_003619851.3 | 96.7 | 429 | 14 | 0 | 1 | 429 | 1 | 429 | 6.00E-192 | 680.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042866.t1 | Q9LVI4 | 36.0 | 419 | 193 | 15 | 1 | 411 | 1 | 352 | 3.5e-39 | 164.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042866.t1 | A0A396HFY8 | 96.7 | 429 | 14 | 0 | 1 | 429 | 1 | 429 | 4.3e-192 | 680.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene042866.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene066964 | MS.gene042866 | PPI |
| MS.gene79795 | MS.gene042866 | PPI |
| MS.gene82105 | MS.gene042866 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042866.t1 | MTR_6g069870 | 96.203 | 395 | 15 | 0 | 35 | 429 | 1 | 395 | 0.0 | 771 |
| MS.gene042866.t1 | MTR_6g069870 | 96.221 | 344 | 13 | 0 | 35 | 378 | 1 | 344 | 0.0 | 671 |
| MS.gene042866.t1 | MTR_6g069870 | 95.975 | 323 | 13 | 0 | 35 | 357 | 1 | 323 | 0.0 | 625 |
| MS.gene042866.t1 | MTR_1g031930 | 30.000 | 420 | 184 | 15 | 1 | 394 | 1 | 336 | 4.98e-32 | 125 |
| MS.gene042866.t1 | MTR_1g031930 | 34.286 | 280 | 109 | 12 | 130 | 391 | 50 | 272 | 5.45e-24 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042866.t1 | AT3G17860 | 39.216 | 408 | 155 | 18 | 1 | 391 | 1 | 332 | 9.74e-55 | 186 |
| MS.gene042866.t1 | AT3G17860 | 38.441 | 372 | 143 | 16 | 34 | 391 | 20 | 319 | 2.63e-44 | 157 |
| MS.gene042866.t1 | AT3G17860 | 38.235 | 374 | 139 | 17 | 35 | 391 | 1 | 299 | 7.60e-44 | 156 |
| MS.gene042866.t1 | AT3G17860 | 38.235 | 374 | 139 | 17 | 35 | 391 | 1 | 299 | 7.60e-44 | 156 |
| MS.gene042866.t1 | AT1G48500 | 34.211 | 418 | 130 | 19 | 1 | 396 | 1 | 295 | 5.02e-38 | 140 |
| MS.gene042866.t1 | AT1G48500 | 33.835 | 399 | 120 | 18 | 1 | 378 | 1 | 276 | 2.33e-33 | 127 |
| MS.gene042866.t1 | AT1G48500 | 32.692 | 364 | 106 | 15 | 35 | 378 | 2 | 246 | 1.46e-27 | 110 |
| MS.gene042866.t1 | AT1G70700 | 41.593 | 113 | 58 | 4 | 125 | 237 | 58 | 162 | 1.10e-12 | 68.2 |
| MS.gene042866.t1 | AT1G70700 | 41.593 | 113 | 58 | 4 | 125 | 237 | 58 | 162 | 1.67e-12 | 67.8 |
| MS.gene042866.t1 | AT1G70700 | 41.593 | 113 | 58 | 4 | 125 | 237 | 58 | 162 | 2.35e-12 | 67.4 |
Find 92 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCATACATGTTCTTCTTAA+AGG | 0.090775 | 6.3:+66674855 | None:intergenic |
| CTTGTTTCCTACTTGCTTTA+TGG | 0.147271 | 6.3:+66676793 | None:intergenic |
| TGTTCTTCTTAAAGGCAAAT+TGG | 0.268291 | 6.3:+66674863 | None:intergenic |
| CCTATTGTTGCTACTTCTAT+AGG | 0.270531 | 6.3:-66675672 | MS.gene042866:CDS |
| GTCTAATCATAAATCATTCT+TGG | 0.289760 | 6.3:-66679389 | MS.gene042866:CDS |
| TTTGATGCAGTTAGGAGTTC+AGG | 0.316880 | 6.3:-66679562 | MS.gene042866:intron |
| GTTACAAACTCACAAACTTT+TGG | 0.317063 | 6.3:-66676644 | MS.gene042866:CDS |
| TGAGAAATCAATGGAGAAAC+TGG | 0.337756 | 6.3:+66674908 | None:intergenic |
| AATCTCTATTCCTTCCAAAA+AGG | 0.344054 | 6.3:-66676003 | MS.gene042866:CDS |
| AACTGTACCACCATAAAATA+TGG | 0.350649 | 6.3:+66676266 | None:intergenic |
| GTTGGAAGCATCTTCTTTCA+TGG | 0.359919 | 6.3:+66683048 | None:intergenic |
| AAATGCCCCTACTAACCATT+TGG | 0.365374 | 6.3:-66675700 | MS.gene042866:CDS |
| CCTGGTTGAATCACATTTGT+AGG | 0.375793 | 6.3:+66675642 | None:intergenic |
| CGGTGTAGTACCATTGGATT+TGG | 0.378173 | 6.3:+66676299 | None:intergenic |
| TTTGAGGGAAGAACAGATAA+AGG | 0.390189 | 6.3:+66676602 | None:intergenic |
| GGAGATTCCAAATGGTTAGT+AGG | 0.395561 | 6.3:+66675693 | None:intergenic |
| GTCACAATAATAAGATCTTT+AGG | 0.396082 | 6.3:-66675729 | MS.gene042866:CDS |
| TCTGATCCAGTGCTGCATTC+AGG | 0.408286 | 6.3:+66674936 | None:intergenic |
| CTGATTGTGCATCAGAACTT+TGG | 0.410761 | 6.3:+66676747 | None:intergenic |
| CAATCTAATCATGTTACTAC+TGG | 0.412033 | 6.3:-66676686 | MS.gene042866:CDS |
| TAAGTCAGTAGAGCCAACTA+TGG | 0.416073 | 6.3:+66676574 | None:intergenic |
| CCTATAGAAGTAGCAACAAT+AGG | 0.423632 | 6.3:+66675672 | None:intergenic |
| TAGTTGGCTCTACTGACTTA+AGG | 0.429024 | 6.3:-66676571 | MS.gene042866:intron |
| CAGTAGTAACATGATTAGAT+TGG | 0.431253 | 6.3:+66676687 | None:intergenic |
| TAGATCAAGAAACAGTGTTA+TGG | 0.431305 | 6.3:-66679464 | MS.gene042866:CDS |
| TTATCTGTTCTTCCCTCAAA+AGG | 0.432397 | 6.3:-66676599 | MS.gene042866:CDS |
| TGAGAGGACTTGGCAATTGA+AGG | 0.441352 | 6.3:+66675849 | None:intergenic |
| GAAGTCATTGGGAGAGAACT+TGG | 0.446941 | 6.3:+66675798 | None:intergenic |
| GAACTTGCATGAGAAGTCAT+TGG | 0.447319 | 6.3:+66675786 | None:intergenic |
| TTGCTACTTGATCCTCCCCT+TGG | 0.449675 | 6.3:+66675903 | None:intergenic |
| GCCTTTAAGAAGAACATGTA+TGG | 0.452229 | 6.3:-66674856 | MS.gene042866:CDS |
| TAGGTCATTGTGAGAGGACT+TGG | 0.454376 | 6.3:+66675839 | None:intergenic |
| GCTTGGCAATGGTGAGGGAT+AGG | 0.455476 | 6.3:+66675959 | None:intergenic |
| GAGATTCCAAATGGTTAGTA+GGG | 0.462551 | 6.3:+66675694 | None:intergenic |
| GAGGTCATTGGAAGAGGGCT+TGG | 0.466655 | 6.3:+66675942 | None:intergenic |
| GTACTTGCATGAGAGGTCAT+TGG | 0.470357 | 6.3:+66675930 | None:intergenic |
| GTTGCTACTTGAACCTCCCT+TGG | 0.472391 | 6.3:+66675758 | None:intergenic |
| TCTAATCATAAATCATTCTT+GGG | 0.475638 | 6.3:-66679388 | MS.gene042866:CDS |
| ATGGATCCAGTAGCTTCATC+TGG | 0.480372 | 6.3:-66679445 | MS.gene042866:CDS |
| TCATGCAAGTTCTTATTCCA+AGG | 0.481899 | 6.3:-66675775 | MS.gene042866:CDS |
| CCCTCAAAAGGTCCCATAGT+TGG | 0.482615 | 6.3:-66676587 | MS.gene042866:CDS |
| AACTTGCATGAGAAGTCATT+GGG | 0.486290 | 6.3:+66675787 | None:intergenic |
| ATATATCCAGATGAAGCTAC+TGG | 0.486559 | 6.3:+66679439 | None:intergenic |
| TAAAATATGGTCAACTGAGC+CGG | 0.491802 | 6.3:+66676279 | None:intergenic |
| CTTGGCAATGGTGAGGGATA+GGG | 0.492080 | 6.3:+66675960 | None:intergenic |
| TTCTTGAATGACATGAATTG+AGG | 0.493289 | 6.3:+66679502 | None:intergenic |
| GCATGAGAGGTCATTGGAAG+AGG | 0.495990 | 6.3:+66675936 | None:intergenic |
| ATCATGTTACTAGCTGGAAA+TGG | 0.499618 | 6.3:-66676044 | MS.gene042866:CDS |
| CTGAGCCGGTGTAGTACCAT+TGG | 0.502183 | 6.3:+66676293 | None:intergenic |
| GTCACAATAATAAGATCCTT+AGG | 0.503685 | 6.3:-66675873 | MS.gene042866:CDS |
| ATCATTCTTGGGTGTGACAC+AGG | 0.505809 | 6.3:-66679377 | MS.gene042866:intron |
| CAACAATAGGAGATTCCAAA+TGG | 0.511822 | 6.3:+66675685 | None:intergenic |
| AACAGATAAAGGTGTGGCAG+AGG | 0.516274 | 6.3:+66676613 | None:intergenic |
| AAGGCAATCATGTTACTAGC+TGG | 0.519440 | 6.3:-66676050 | MS.gene042866:CDS |
| CTTCTTAAAGGCAAATTGGT+GGG | 0.529876 | 6.3:+66674867 | None:intergenic |
| TCTTCTTTCATGGTAGTCCA+TGG | 0.530904 | 6.3:+66683058 | None:intergenic |
| CTTGGCAATTGAAGGTCCTA+AGG | 0.532204 | 6.3:+66675857 | None:intergenic |
| TGGTTAGTAGGGGCATTTGA+AGG | 0.535343 | 6.3:+66675705 | None:intergenic |
| AAATCACCTGAATGCAGCAC+TGG | 0.539481 | 6.3:-66674942 | MS.gene042866:CDS |
| CATGCAAGTTCTTATTCCAA+GGG | 0.544378 | 6.3:-66675774 | MS.gene042866:CDS |
| ACTTGAACTTGAGAAATCAA+TGG | 0.545383 | 6.3:+66674899 | None:intergenic |
| GCAAAGGTGAGAGATGAGAT+AGG | 0.557521 | 6.3:+66675820 | None:intergenic |
| AGAGGGCTTGGCAATGGTGA+GGG | 0.558559 | 6.3:+66675954 | None:intergenic |
| TGGTTGAGTACTTGCATGAG+AGG | 0.558968 | 6.3:+66675923 | None:intergenic |
| TCTTCTTAAAGGCAAATTGG+TGG | 0.560082 | 6.3:+66674866 | None:intergenic |
| CATGAGAGGTCATTGGAAGA+GGG | 0.576369 | 6.3:+66675937 | None:intergenic |
| TGTGCATCAGAACTTTGGAT+AGG | 0.578539 | 6.3:+66676752 | None:intergenic |
| TGAATGACATGAATTGAGGA+AGG | 0.578633 | 6.3:+66679506 | None:intergenic |
| ATTGGAAGAGGGCTTGGCAA+TGG | 0.579330 | 6.3:+66675948 | None:intergenic |
| ATGAGATAGGTCATTGTGAG+AGG | 0.585630 | 6.3:+66675833 | None:intergenic |
| AAGAGGGCTTGGCAATGGTG+AGG | 0.587772 | 6.3:+66675953 | None:intergenic |
| CATATGGAAAGAGAATTCCT+TGG | 0.590006 | 6.3:-66683100 | MS.gene042866:CDS |
| GCAAGTTCTTATTCCAAGGG+AGG | 0.592795 | 6.3:-66675771 | MS.gene042866:CDS |
| CTTAAAGGCAAATTGGTGGG+TGG | 0.595542 | 6.3:+66674870 | None:intergenic |
| CCTACAAATGTGATTCAACC+AGG | 0.596560 | 6.3:-66675642 | MS.gene042866:intron |
| TCATGCAAGTACTCAACCAA+GGG | 0.597542 | 6.3:-66675919 | MS.gene042866:CDS |
| AAGTCAGTAGAGCCAACTAT+GGG | 0.597778 | 6.3:+66676575 | None:intergenic |
| TGATGACATCTCTCCTGAGA+AGG | 0.609439 | 6.3:-66676235 | MS.gene042866:intron |
| CTCATGCAAGTACTCAACCA+AGG | 0.611643 | 6.3:-66675920 | MS.gene042866:CDS |
| AGGAATTGTTCCAAATCCAA+TGG | 0.614409 | 6.3:-66676309 | MS.gene042866:CDS |
| AATTTGGCCATAAAGCAAGT+AGG | 0.634556 | 6.3:-66676800 | MS.gene042866:CDS |
| GCAATGGTGAGGGATAGGGT+TGG | 0.638300 | 6.3:+66675964 | None:intergenic |
| AAGCAAGTAGGAAACAAGCA+TGG | 0.639462 | 6.3:-66676788 | MS.gene042866:CDS |
| ATTGGGAGAGAACTTGGCAA+AGG | 0.651165 | 6.3:+66675804 | None:intergenic |
| CAAATCCAATGGTACTACAC+CGG | 0.651773 | 6.3:-66676298 | MS.gene042866:CDS |
| AGATTCCAAATGGTTAGTAG+GGG | 0.670933 | 6.3:+66675695 | None:intergenic |
| GCAAGTACTCAACCAAGGGG+AGG | 0.678862 | 6.3:-66675915 | MS.gene042866:CDS |
| TTAGGAGTTCAGGAATGCAG+TGG | 0.686957 | 6.3:-66679552 | MS.gene042866:CDS |
| TGTTACTACTGGAATCAACA+TGG | 0.687462 | 6.3:-66676675 | MS.gene042866:CDS |
| TCCAACAAACCAAAAGACCA+AGG | 0.694197 | 6.3:-66683031 | MS.gene042866:intron |
| GGGAAGAACAGATAAAGGTG+TGG | 0.711636 | 6.3:+66676607 | None:intergenic |
| CATGCAAGTACTCAACCAAG+GGG | 0.756359 | 6.3:-66675918 | MS.gene042866:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 66674842 | 66683119 | 66674842 | ID=MS.gene042866 |
| chr6.3 | mRNA | 66674842 | 66683119 | 66674842 | ID=MS.gene042866.t1;Parent=MS.gene042866 |
| chr6.3 | exon | 66683032 | 66683119 | 66683032 | ID=MS.gene042866.t1.exon1;Parent=MS.gene042866.t1 |
| chr6.3 | CDS | 66683032 | 66683119 | 66683032 | ID=cds.MS.gene042866.t1;Parent=MS.gene042866.t1 |
| chr6.3 | exon | 66679378 | 66679574 | 66679378 | ID=MS.gene042866.t1.exon2;Parent=MS.gene042866.t1 |
| chr6.3 | CDS | 66679378 | 66679574 | 66679378 | ID=cds.MS.gene042866.t1;Parent=MS.gene042866.t1 |
| chr6.3 | exon | 66676572 | 66676825 | 66676572 | ID=MS.gene042866.t1.exon3;Parent=MS.gene042866.t1 |
| chr6.3 | CDS | 66676572 | 66676825 | 66676572 | ID=cds.MS.gene042866.t1;Parent=MS.gene042866.t1 |
| chr6.3 | exon | 66676236 | 66676335 | 66676236 | ID=MS.gene042866.t1.exon4;Parent=MS.gene042866.t1 |
| chr6.3 | CDS | 66676236 | 66676335 | 66676236 | ID=cds.MS.gene042866.t1;Parent=MS.gene042866.t1 |
| chr6.3 | exon | 66675643 | 66676075 | 66675643 | ID=MS.gene042866.t1.exon5;Parent=MS.gene042866.t1 |
| chr6.3 | CDS | 66675643 | 66676075 | 66675643 | ID=cds.MS.gene042866.t1;Parent=MS.gene042866.t1 |
| chr6.3 | exon | 66675316 | 66675376 | 66675316 | ID=MS.gene042866.t1.exon6;Parent=MS.gene042866.t1 |
| chr6.3 | CDS | 66675316 | 66675376 | 66675316 | ID=cds.MS.gene042866.t1;Parent=MS.gene042866.t1 |
| chr6.3 | exon | 66674842 | 66674998 | 66674842 | ID=MS.gene042866.t1.exon7;Parent=MS.gene042866.t1 |
| chr6.3 | CDS | 66674842 | 66674998 | 66674842 | ID=cds.MS.gene042866.t1;Parent=MS.gene042866.t1 |
| Gene Sequence |
| Protein sequence |
