Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044787.t1 | XP_013455799.1 | 98.9 | 544 | 6 | 0 | 1 | 544 | 1 | 544 | 6.50E-308 | 1065.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044787.t1 | Q8VCM8 | 33.2 | 539 | 304 | 15 | 20 | 532 | 28 | 536 | 2.3e-67 | 258.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044787.t1 | A0A072UVE5 | 98.9 | 544 | 6 | 0 | 1 | 544 | 1 | 544 | 4.7e-308 | 1065.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044787 | MS.gene072688 | PPI |
| MS.gene044787 | MS.gene28821 | PPI |
| MS.gene044787 | MS.gene07068 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044787.t1 | MTR_4g052530 | 98.897 | 544 | 6 | 0 | 1 | 544 | 1 | 544 | 0.0 | 1102 |
| MS.gene044787.t1 | MTR_4g052530 | 95.390 | 564 | 6 | 1 | 1 | 544 | 1 | 564 | 0.0 | 1090 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044787.t1 | AT3G44330 | 73.406 | 549 | 137 | 4 | 1 | 544 | 1 | 545 | 0.0 | 818 |
Find 121 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACATTGACACCGTGTTAT+TGG | 0.199855 | 4.1:-45489197 | MS.gene044787:CDS |
| AATTGTGCTCTTCAGTTTCT+TGG | 0.209041 | 4.1:-45483311 | MS.gene044787:CDS |
| TCAAGTGTTGCAGAAGATTT+AGG | 0.221551 | 4.1:-45486804 | MS.gene044787:CDS |
| CTTTGCGGACAACAGTAATT+TGG | 0.260388 | 4.1:-45484319 | MS.gene044787:CDS |
| CACATTCTTCAGCGGTTCTT+TGG | 0.280526 | 4.1:+45490359 | None:intergenic |
| TTCTAGAATTTGTTTAATAA+AGG | 0.282297 | 4.1:+45486931 | None:intergenic |
| GGTGGAGATCCAACTTTCTT+TGG | 0.305838 | 4.1:+45488871 | None:intergenic |
| CCGGTGCTCTGTAGCAATTC+AGG | 0.309146 | 4.1:+45485655 | None:intergenic |
| CTCAACATGTCCTTTGTTCA+AGG | 0.340657 | 4.1:-45492017 | MS.gene044787:intron |
| TCCACCCCTTACAAATATTC+AGG | 0.348535 | 4.1:-45488854 | MS.gene044787:intron |
| TGGCATCATATGACACCTTT+GGG | 0.352355 | 4.1:-45487857 | MS.gene044787:CDS |
| GGACAATACAATCTGCTGTT+TGG | 0.356916 | 4.1:-45487641 | MS.gene044787:CDS |
| GATGAAGACGAGTGCGATTA+CGG | 0.357448 | 4.1:+45492226 | None:intergenic |
| TGTTTGCAGGGATGGTTGCC+AGG | 0.368228 | 4.1:-45487927 | MS.gene044787:intron |
| TGGTGCTACTCGAGGTGTTC+GGG | 0.368397 | 4.1:+45484254 | None:intergenic |
| GCTCATGGGAAAATGATCTA+TGG | 0.378313 | 4.1:-45486982 | MS.gene044787:CDS |
| AGCACCGGTGGTTTGGTTGA+TGG | 0.380580 | 4.1:-45485640 | MS.gene044787:CDS |
| CGGGTTCCATTGTAGTTGTA+AGG | 0.382485 | 4.1:+45487602 | None:intergenic |
| GATATCTCCGGTGTTCCGTT+TGG | 0.384689 | 4.1:-45492137 | MS.gene044787:CDS |
| GACAATACAATCTGCTGTTT+GGG | 0.385785 | 4.1:-45487640 | MS.gene044787:CDS |
| TTGTATTGTCCTCTTGTCTT+CGG | 0.385886 | 4.1:+45487653 | None:intergenic |
| ACATCTGCTGTAGGTAGCTT+GGG | 0.388643 | 4.1:-45485740 | MS.gene044787:intron |
| CTTGCTGTTGCTTCTAGTGT+TGG | 0.389075 | 4.1:-45483344 | MS.gene044787:CDS |
| GACATCTGCTGTAGGTAGCT+TGG | 0.390955 | 4.1:-45485741 | MS.gene044787:intron |
| CAAACACCTGAATATTTGTA+AGG | 0.392964 | 4.1:+45488848 | None:intergenic |
| GCTATTTGCTTAGATAGTAT+TGG | 0.394414 | 4.1:-45487004 | MS.gene044787:CDS |
| GTGGCATCATATGACACCTT+TGG | 0.400492 | 4.1:-45487858 | MS.gene044787:CDS |
| TTGCTGTTGCTTCTAGTGTT+GGG | 0.402441 | 4.1:-45483343 | MS.gene044787:CDS |
| ATTACCCACCATTGCGATAG+TGG | 0.410780 | 4.1:-45487877 | MS.gene044787:CDS |
| GCTACAGAGCACCGGTGGTT+TGG | 0.416449 | 4.1:-45485647 | MS.gene044787:CDS |
| ATCCTTCTTATATTCGATCA+TGG | 0.419096 | 4.1:-45484290 | MS.gene044787:CDS |
| CTACAACGAGAAGTGCTAGA+TGG | 0.420471 | 4.1:-45483790 | MS.gene044787:CDS |
| TCCTCAAAAGCAAAATAAAC+AGG | 0.426831 | 4.1:+45489223 | None:intergenic |
| TATTGGTGGTAGCAAGCCTC+TGG | 0.428171 | 4.1:-45490460 | MS.gene044787:CDS |
| AACAGGATGTATCACCTTAC+GGG | 0.437949 | 4.1:+45487583 | None:intergenic |
| TCCAACTTTCTTTGGTTCAG+TGG | 0.446635 | 4.1:+45488879 | None:intergenic |
| CCGGTGGTTTGGTTGATGGC+AGG | 0.449964 | 4.1:-45485636 | MS.gene044787:intron |
| ATTGGTGGTAGCAAGCCTCT+GGG | 0.454869 | 4.1:-45490459 | MS.gene044787:CDS |
| TCTTATGCTTCAGATTAACT+TGG | 0.460383 | 4.1:+45486778 | None:intergenic |
| AGCACAGCTGAACATATATC+AGG | 0.467914 | 4.1:-45483740 | MS.gene044787:intron |
| ATGGTGCTACTCGAGGTGTT+CGG | 0.469594 | 4.1:+45484253 | None:intergenic |
| CTTAGATAGTATTGGCTCAT+GGG | 0.470218 | 4.1:-45486996 | MS.gene044787:CDS |
| CTGCTGTTTGGGCTAACGTC+TGG | 0.478814 | 4.1:-45487629 | MS.gene044787:CDS |
| TGGTGGTAGCAAGCCTCTGG+GGG | 0.481056 | 4.1:-45490457 | MS.gene044787:CDS |
| GCTTAGATAGTATTGGCTCA+TGG | 0.482039 | 4.1:-45486997 | MS.gene044787:CDS |
| TGACACCTTTGGGGCTGCTC+CGG | 0.485813 | 4.1:-45487847 | MS.gene044787:intron |
| CGGAGATATCGTATTGGATA+AGG | 0.488170 | 4.1:+45492150 | None:intergenic |
| GCCACTGAACCAAAGAAAGT+TGG | 0.489173 | 4.1:-45488880 | MS.gene044787:CDS |
| AAATTTACTCACAGGTAAGT+TGG | 0.502805 | 4.1:+45490305 | None:intergenic |
| CTGTTTGGGCTAACGTCTGG+TGG | 0.508872 | 4.1:-45487626 | MS.gene044787:CDS |
| AGTACGAGAGAGATCAGCAC+TGG | 0.516095 | 4.1:+45492064 | None:intergenic |
| TCACCATCTGTCTTCAGCCC+TGG | 0.516595 | 4.1:+45487909 | None:intergenic |
| AACCATGATCGAATATAAGA+AGG | 0.520778 | 4.1:+45484288 | None:intergenic |
| CCGCCAGTAGTTGCAGTAGC+AGG | 0.521090 | 4.1:+45489145 | None:intergenic |
| AAGCATATTTATGGGCATCA+AGG | 0.529020 | 4.1:-45484357 | MS.gene044787:CDS |
| CAGCCTGCTACTGCAACTAC+TGG | 0.535144 | 4.1:-45489148 | MS.gene044787:CDS |
| TACAACGAGAAGTGCTAGAT+GGG | 0.535738 | 4.1:-45483789 | MS.gene044787:CDS |
| GCGAGTAGTAATCATGATGA+TGG | 0.542254 | 4.1:-45490384 | MS.gene044787:CDS |
| ACTTGTACGACAGAGTATAT+TGG | 0.551265 | 4.1:-45490477 | MS.gene044787:intron |
| TCGTTGTAGATTAACTTCAT+CGG | 0.552366 | 4.1:+45483804 | None:intergenic |
| ACCTGAATATTTGTAAGGGG+TGG | 0.552902 | 4.1:+45488853 | None:intergenic |
| AAAGAACATACAGATCTTTG+CGG | 0.553176 | 4.1:-45484334 | MS.gene044787:CDS |
| CGGAGTCGAAGAGGATTTCG+CGG | 0.558974 | 4.1:+45492246 | None:intergenic |
| ATCAAGAAGAATGATGCCTC+TGG | 0.560007 | 4.1:-45489172 | MS.gene044787:CDS |
| TGATGCCACTATCGCAATGG+TGG | 0.566504 | 4.1:+45487872 | None:intergenic |
| CAGCAAGTCAAACGTGACAC+TGG | 0.568984 | 4.1:+45483360 | None:intergenic |
| AAACACCTGAATATTTGTAA+GGG | 0.570541 | 4.1:+45488849 | None:intergenic |
| CTTATCCAATACGATATCTC+CGG | 0.573897 | 4.1:-45492149 | MS.gene044787:CDS |
| ACGAGATCCAAACGGAACAC+CGG | 0.575932 | 4.1:+45492130 | None:intergenic |
| TATTCGAATCCGAAGACAAG+AGG | 0.577516 | 4.1:-45487662 | MS.gene044787:CDS |
| CTGCTACTGCAACTACTGGC+GGG | 0.584909 | 4.1:-45489144 | MS.gene044787:intron |
| AAGAGGATTTCGCGGTTGCG+AGG | 0.587745 | 4.1:+45492254 | None:intergenic |
| AATTATCGAACCTTGAACAA+AGG | 0.589660 | 4.1:+45492007 | None:intergenic |
| GTTTGCAGGGATGGTTGCCA+GGG | 0.589899 | 4.1:-45487926 | MS.gene044787:intron |
| CGAGTAGTAATCATGATGAT+GGG | 0.590493 | 4.1:-45490383 | MS.gene044787:CDS |
| GGAAGCGATAGCAATGGAAG+TGG | 0.595409 | 4.1:-45487728 | MS.gene044787:CDS |
| TATACTTACCGCAAGAGACT+CGG | 0.595460 | 4.1:+45485492 | None:intergenic |
| GACCCTCTCTGAACTCTCTA+CGG | 0.595807 | 4.1:-45485680 | MS.gene044787:CDS |
| CAACTACAATGGAACCCGTA+AGG | 0.596945 | 4.1:-45487597 | MS.gene044787:intron |
| GAACACCGGAGATATCGTAT+TGG | 0.602447 | 4.1:+45492144 | None:intergenic |
| ATATATGTTCAGCTGTGCTG+TGG | 0.602643 | 4.1:+45483744 | None:intergenic |
| TGTTTGGGCTAACGTCTGGT+GGG | 0.605689 | 4.1:-45487625 | MS.gene044787:CDS |
| TTGGTGGTAGCAAGCCTCTG+GGG | 0.607148 | 4.1:-45490458 | MS.gene044787:CDS |
| GTGCGATTACGGAGTCGAAG+AGG | 0.609499 | 4.1:+45492237 | None:intergenic |
| TGCAGTAGCAGGCTGACCAG+AGG | 0.611293 | 4.1:+45489156 | None:intergenic |
| GGTGGTAGCAAGCCTCTGGG+GGG | 0.611979 | 4.1:-45490456 | MS.gene044787:CDS |
| CTAGCGAGACGAGATCCAAA+CGG | 0.613160 | 4.1:+45492122 | None:intergenic |
| GCCTGCGTCGACCTCTGTGA+CGG | 0.615505 | 4.1:-45492197 | MS.gene044787:CDS |
| ACTAATTGCCGAGTCTCTTG+CGG | 0.617297 | 4.1:-45485500 | MS.gene044787:intron |
| TAATTGTAGGCGTTATCAGT+GGG | 0.618232 | 4.1:-45487749 | MS.gene044787:intron |
| GGTGGGCCTTACAACTACAA+TGG | 0.619306 | 4.1:-45487608 | MS.gene044787:CDS |
| TGCCGTAGAGAGTTCAGAGA+GGG | 0.620583 | 4.1:+45485678 | None:intergenic |
| AGAACCGCTGAAGAATGTGT+TGG | 0.621201 | 4.1:-45490355 | MS.gene044787:CDS |
| CCTGCCATCAACCAAACCAC+CGG | 0.622459 | 4.1:+45485636 | None:intergenic |
| CTTCTTGATATCCAATAACA+CGG | 0.623420 | 4.1:+45489186 | None:intergenic |
| CGAAATGAAGAGAAGCAGCA+TGG | 0.624639 | 4.1:+45492090 | None:intergenic |
| AACACCTGAATATTTGTAAG+GGG | 0.627059 | 4.1:+45488850 | None:intergenic |
| GCCGTCACAGAGGTCGACGC+AGG | 0.628037 | 4.1:+45492196 | None:intergenic |
| GTAATTGTAGGCGTTATCAG+TGG | 0.631414 | 4.1:-45487750 | MS.gene044787:intron |
| CCTGAATTGCTACAGAGCAC+CGG | 0.633915 | 4.1:-45485655 | MS.gene044787:CDS |
| ACATACCGGAGCAGCCCCAA+AGG | 0.637663 | 4.1:+45487842 | None:intergenic |
| CGACGGTGGCGCCGTCACAG+AGG | 0.638908 | 4.1:+45492186 | None:intergenic |
| GGCATCATATGACACCTTTG+GGG | 0.639014 | 4.1:-45487856 | MS.gene044787:CDS |
| CCTGCTACTGCAACTACTGG+CGG | 0.643000 | 4.1:-45489145 | MS.gene044787:CDS |
| TGTACGACAGAGTATATTGG+TGG | 0.643856 | 4.1:-45490474 | MS.gene044787:intron |
| GATGCCACTATCGCAATGGT+GGG | 0.644935 | 4.1:+45487873 | None:intergenic |
| TAGCAATGGAAGTGGCATTG+TGG | 0.650973 | 4.1:-45487720 | MS.gene044787:CDS |
| TCATCCAACACATTCTTCAG+CGG | 0.659873 | 4.1:+45490351 | None:intergenic |
| AGAGGTCGACGCAGGCGACG+AGG | 0.660068 | 4.1:+45492204 | None:intergenic |
| GAATTGCTACAGAGCACCGG+TGG | 0.660576 | 4.1:-45485652 | MS.gene044787:CDS |
| TTGCCAGGGCTGAAGACAGA+TGG | 0.664980 | 4.1:-45487912 | MS.gene044787:CDS |
| GTTTCTTGGTCATCACTACG+AGG | 0.684593 | 4.1:-45483297 | MS.gene044787:CDS |
| TTTCTTGGTCATCACTACGA+GGG | 0.687354 | 4.1:-45483296 | None:intergenic |
| AGATATCGTATTGGATAAGG+CGG | 0.692129 | 4.1:+45492153 | None:intergenic |
| GTACGAGAGAGATCAGCACT+GGG | 0.694728 | 4.1:+45492065 | None:intergenic |
| GCGGTAAACATCGACGACGG+TGG | 0.697144 | 4.1:+45492172 | None:intergenic |
| ATATGATGCCACTATCGCAA+TGG | 0.698292 | 4.1:+45487869 | None:intergenic |
| GACATGTTGAGTTCACGAAG+AGG | 0.701734 | 4.1:+45492029 | None:intergenic |
| TCAGTGGGAAGCGATAGCAA+TGG | 0.705355 | 4.1:-45487734 | MS.gene044787:CDS |
| GTGCCGTAGAGAGTTCAGAG+AGG | 0.752779 | 4.1:+45485677 | None:intergenic |
| AAGGCGGTAAACATCGACGA+CGG | 0.772440 | 4.1:+45492169 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 45483297 | 45492297 | 45483297 | ID=MS.gene044787 |
| chr4.1 | mRNA | 45483297 | 45492297 | 45483297 | ID=MS.gene044787.t1;Parent=MS.gene044787 |
| chr4.1 | exon | 45492018 | 45492297 | 45492018 | ID=MS.gene044787.t1.exon1;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45492018 | 45492297 | 45492018 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45490317 | 45490486 | 45490317 | ID=MS.gene044787.t1.exon2;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45490317 | 45490486 | 45490317 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45489145 | 45489248 | 45489145 | ID=MS.gene044787.t1.exon3;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45489145 | 45489248 | 45489145 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45488855 | 45488921 | 45488855 | ID=MS.gene044787.t1.exon4;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45488855 | 45488921 | 45488855 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45487848 | 45487940 | 45487848 | ID=MS.gene044787.t1.exon5;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45487848 | 45487940 | 45487848 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45487598 | 45487762 | 45487598 | ID=MS.gene044787.t1.exon6;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45487598 | 45487762 | 45487598 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45486931 | 45487071 | 45486931 | ID=MS.gene044787.t1.exon7;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45486931 | 45487071 | 45486931 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45486755 | 45486832 | 45486755 | ID=MS.gene044787.t1.exon8;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45486755 | 45486832 | 45486755 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45485637 | 45485749 | 45485637 | ID=MS.gene044787.t1.exon9;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45485637 | 45485749 | 45485637 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45485501 | 45485561 | 45485501 | ID=MS.gene044787.t1.exon10;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45485501 | 45485561 | 45485501 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45484212 | 45484379 | 45484212 | ID=MS.gene044787.t1.exon11;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45484212 | 45484379 | 45484212 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45483741 | 45483842 | 45483741 | ID=MS.gene044787.t1.exon12;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45483741 | 45483842 | 45483741 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| chr4.1 | exon | 45483297 | 45483386 | 45483297 | ID=MS.gene044787.t1.exon13;Parent=MS.gene044787.t1 |
| chr4.1 | CDS | 45483297 | 45483386 | 45483297 | ID=cds.MS.gene044787.t1;Parent=MS.gene044787.t1 |
| Gene Sequence |
| Protein sequence |
