AlfalfaGEDB | MS.gene045262

M. sativa cultivar XinJiangDaYe / MS.gene045262

KEGG: K12946
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005787 GO:0006465 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009987 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0031224 GO:0032991 GO:0034641 GO:0043170 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:0098796 GO:0110165 GO:0140096 GO:1901564 GO:1902494 GO:1905368
PFAM: PF06645.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045262.t1	XP_003601799.1	98.9	93	1	0	1	93	1	93	7.70E-44	186

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045262.t1	Q944J0	71.6	88	25	0	1	88	1	88	6.4e-31	134.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045262.t1	G7JC07	98.9	93	1	0	1	93	1	93	5.6e-44	186.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene045262	MS.gene86381	PPI
MS.gene045262	MS.gene89036	PPI
MS.gene045262	MS.gene36215	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045262.t1	MTR_3g085510	98.925	93	1	0	1	93	1	93	2.23e-64	189
MS.gene045262.t1	MTR_8g080380	78.495	93	20	0	1	93	92	184	3.90e-52	161

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045262.t1	AT4G40042	79.747	79	16	1	79	1	79	1.26e-43	137
MS.gene045262.t1	AT2G22425	71.591	88	25	1	88	1	88	1.76e-41	131
MS.gene045262.t1	AT2G22425	71.591	88	25	1	88	1	88	1.76e-41	131

Find 24 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGAGGATGGCGGTTGAAAA+AGG	0.272451	3.2:-73346891	None:intergenic
CATTGGTGACTGTCCCAAAT+TGG	0.297025	3.2:+73346867	MS.gene045262:CDS
GCATTTGCTGTGGTTGCATT+TGG	0.308000	3.2:+73346776	MS.gene045262:CDS
TTCTTCTTTGAATTCACAAT+AGG	0.417935	3.2:-73346965	None:intergenic
GAAGAAACCCATTAAGAAGT+AGG	0.438658	3.2:+73346982	MS.gene045262:CDS
TCTGCATTAGCTTCTCTGCT+AGG	0.440951	3.2:-73346742	None:intergenic
ATAGGAGATAGCTGTGGCTT+CGG	0.450784	3.2:-73346947	None:intergenic
GCTGTGGTTGCATTTGGAAC+GGG	0.459654	3.2:+73346782	MS.gene045262:CDS
TGCTGTGGTTGCATTTGGAA+CGG	0.510809	3.2:+73346781	MS.gene045262:CDS
AAGAAACCCATTAAGAAGTA+GGG	0.531311	3.2:+73346983	MS.gene045262:CDS
TTCATCAGATGGATTGGCAA+GGG	0.536217	3.2:+73346714	None:intergenic
CCGCCATCCTCTCAAGTGGT+TGG	0.536748	3.2:+73346901	MS.gene045262:CDS
TCAACCGCCATCCTCTCAAG+TGG	0.553865	3.2:+73346897	MS.gene045262:CDS
GATCCAACCACTTGAGAGGA+TGG	0.566183	3.2:-73346904	None:intergenic
CTTCATCAGATGGATTGGCA+AGG	0.566563	3.2:+73346713	None:intergenic
CTTGGATCCAACCACTTGAG+AGG	0.567895	3.2:-73346908	None:intergenic
AATGTTGCTTGCATTTGCTG+TGG	0.601891	3.2:+73346766	MS.gene045262:CDS
ACGATGATGCTCATATATGC+TGG	0.624836	3.2:+73346827	MS.gene045262:CDS
TGGTGTTGTTCTCACCACAT+TGG	0.662379	3.2:+73346850	MS.gene045262:CDS
CAAGTGGTTGGATCCAAGTG+AGG	0.664517	3.2:+73346913	MS.gene045262:CDS
ATGATGCTCATATATGCTGG+TGG	0.699379	3.2:+73346830	MS.gene045262:CDS
CCAACCACTTGAGAGGATGG+CGG	0.730520	3.2:-73346901	None:intergenic
ATTTGGGACAGTCACCAATG+TGG	0.730637	3.2:-73346864	None:intergenic
TTCACAATAGGAGATAGCTG+TGG	0.732645	3.2:-73346953	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTCTTCTTTGAATTCACAAT+AGG	-	chr3.2:73346968-73346987	None:intergenic	25.0%
	ACGATGATGCTCATATATGC+TGG	+	chr3.2:73346827-73346846	MS.gene045262:CDS	40.0%
	ATGATGCTCATATATGCTGG+TGG	+	chr3.2:73346830-73346849	MS.gene045262:CDS	40.0%
	GGTTGAAAAAGGGCCAATTT+GGG	-	chr3.2:73346883-73346902	None:intergenic	40.0%
	TTCACAATAGGAGATAGCTG+TGG	-	chr3.2:73346956-73346975	None:intergenic	40.0%
!	AATGTTGCTTGCATTTGCTG+TGG	+	chr3.2:73346766-73346785	MS.gene045262:CDS	40.0%
	ATAGGAGATAGCTGTGGCTT+CGG	-	chr3.2:73346950-73346969	None:intergenic	45.0%
	CGGTTGAAAAAGGGCCAATT+TGG	-	chr3.2:73346884-73346903	None:intergenic	45.0%
	TGCTGTGGTTGCATTTGGAA+CGG	+	chr3.2:73346781-73346800	MS.gene045262:CDS	45.0%
!	AGAGGATGGCGGTTGAAAAA+GGG	-	chr3.2:73346893-73346912	None:intergenic	45.0%
!	ATTTGGGACAGTCACCAATG+TGG	-	chr3.2:73346867-73346886	None:intergenic	45.0%
!	CATTGGTGACTGTCCCAAAT+TGG	+	chr3.2:73346867-73346886	MS.gene045262:CDS	45.0%
!	GGATGCTTTTCTACCTCACT+TGG	-	chr3.2:73346929-73346948	None:intergenic	45.0%
!	TCTGCATTAGCTTCTCTGCT+AGG	-	chr3.2:73346745-73346764	None:intergenic	45.0%
!!	GCATTTGCTGTGGTTGCATT+TGG	+	chr3.2:73346776-73346795	MS.gene045262:CDS	45.0%
!!	TGGTGTTGTTCTCACCACAT+TGG	+	chr3.2:73346850-73346869	MS.gene045262:CDS	45.0%
	CAAGTGGTTGGATCCAAGTG+AGG	+	chr3.2:73346913-73346932	MS.gene045262:CDS	50.0%
	CTTGGATCCAACCACTTGAG+AGG	-	chr3.2:73346911-73346930	None:intergenic	50.0%
	GATCCAACCACTTGAGAGGA+TGG	-	chr3.2:73346907-73346926	None:intergenic	50.0%
!	GAGAGGATGGCGGTTGAAAA+AGG	-	chr3.2:73346894-73346913	None:intergenic	50.0%
!	GCTGTGGTTGCATTTGGAAC+GGG	+	chr3.2:73346782-73346801	MS.gene045262:CDS	50.0%
	CCAACCACTTGAGAGGATGG+CGG	-	chr3.2:73346904-73346923	None:intergenic	55.0%
	TCAACCGCCATCCTCTCAAG+TGG	+	chr3.2:73346897-73346916	MS.gene045262:CDS	55.0%
	CCGCCATCCTCTCAAGTGGT+TGG	+	chr3.2:73346901-73346920	MS.gene045262:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	73346722	73347003	73346722	ID=MS.gene045262
chr3.2	mRNA	73346722	73347003	73346722	ID=MS.gene045262.t1;Parent=MS.gene045262
chr3.2	exon	73346722	73347003	73346722	ID=MS.gene045262.t1.exon1;Parent=MS.gene045262.t1
chr3.2	CDS	73346722	73347003	73346722	ID=cds.MS.gene045262.t1;Parent=MS.gene045262.t1

Gene Sequence	>MS.gene045262 ATGGATTGGCAAGGGCAAAACCTAGCAGAGAAGCTAATGCAGATAATGTTGCTTGCATTTGCTGTGGTTGCATTTGGAACGGGATATCTTACAGCTTCTTTCAAGACGATGATGCTCATATATGCTGGTGGTGTTGTTCTCACCACATTGGTGACTGTCCCAAATTGGCCCTTTTTCAACCGCCATCCTCTCAAGTGGTTGGATCCAAGTGAGGTAGAAAAGCATCCGAAGCCACAGCTATCTCCTATTGTGAATTCAAAGAAGAAACCCATTAAGAAGTAG
Protein sequence	>MS.gene045262.t1 MDWQGQNLAEKLMQIMLLAFAVVAFGTGYLTASFKTMMLIYAGGVVLTTLVTVPNWPFFNRHPLKWLDPSEVEKHPKPQLSPIVNSKKKPIKK