Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04609.t1 | XP_003625240.2 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 2.60E-64 | 254.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04609.t1 | G7KVB6 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 1.9e-64 | 254.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene04609 | MS.gene31060 | PPI |
| MS.gene52644 | MS.gene04609 | PPI |
| MS.gene04609 | MS.gene017971 | PPI |
| MS.gene04609 | MS.gene032141 | PPI |
| MS.gene017971 | MS.gene04609 | PPI |
| MS.gene04609 | MS.gene78355 | PPI |
| MS.gene04609 | MS.gene52644 | PPI |
| MS.gene78355 | MS.gene04609 | PPI |
| MS.gene032141 | MS.gene04609 | PPI |
| MS.gene04609 | MS.gene072389 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04609.t1 | MTR_7g092970 | 98.529 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 9.50e-94 | 266 |
| MS.gene04609.t1 | MTR_2g098560 | 58.394 | 137 | 53 | 4 | 1 | 136 | 1 | 134 | 6.57e-40 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04609.t1 | AT3G57930 | 51.748 | 143 | 56 | 5 | 1 | 135 | 1 | 138 | 1.09e-26 | 97.4 |
| MS.gene04609.t1 | AT3G57930 | 51.748 | 143 | 56 | 5 | 1 | 135 | 1 | 138 | 1.09e-26 | 97.4 |
| MS.gene04609.t1 | AT2G42190 | 45.652 | 138 | 72 | 2 | 1 | 135 | 1 | 138 | 3.61e-22 | 85.9 |
| MS.gene04609.t1 | AT2G42190 | 45.652 | 138 | 72 | 2 | 1 | 135 | 1 | 138 | 3.61e-22 | 85.9 |
| MS.gene04609.t1 | AT2G42190 | 45.652 | 138 | 72 | 2 | 1 | 135 | 1 | 138 | 3.61e-22 | 85.9 |
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTAATCACTGTGAATAAA+AGG | 0.302586 | 7.3:+17600904 | MS.gene04609:CDS |
| TCATCCGTGACCGGCTTTAA+TGG | 0.312272 | 7.3:-17600795 | None:intergenic |
| AAAGATGAGGAGAACGTAAA+TGG | 0.358180 | 7.3:+17600852 | MS.gene04609:CDS |
| GAACCAATGAAAGAGGAAAA+TGG | 0.383625 | 7.3:+17600963 | MS.gene04609:CDS |
| TCTACGGGATTCCGACAAAA+TGG | 0.386285 | 7.3:+17601023 | MS.gene04609:CDS |
| ATGGGTAGAGGTCGAGGAAA+GGG | 0.429359 | 7.3:+17600693 | MS.gene04609:CDS |
| GTAAATGGTAATGTTTCTAA+TGG | 0.448458 | 7.3:+17600867 | MS.gene04609:CDS |
| CCTCACCGTGCTGCTGAAGC+TGG | 0.469138 | 7.3:+17601065 | MS.gene04609:CDS |
| AAAGATAGAACCAATGAAAG+AGG | 0.492528 | 7.3:+17600956 | MS.gene04609:CDS |
| TCACTGTGAATAAAAGGAAA+AGG | 0.498884 | 7.3:+17600910 | MS.gene04609:CDS |
| TTGACTATGGGTAGAGGTCG+AGG | 0.519155 | 7.3:+17600687 | None:intergenic |
| TGAATAAAAGGAAAAGGAAG+AGG | 0.531731 | 7.3:+17600916 | MS.gene04609:CDS |
| TGGTGATTCAGTGAAGTCTA+CGG | 0.537967 | 7.3:+17601007 | MS.gene04609:CDS |
| CACCAGGGCTAGACTTAGCT+CGG | 0.543586 | 7.3:-17600989 | None:intergenic |
| GATTCCGACAAAATGGCAGC+AGG | 0.563142 | 7.3:+17601030 | MS.gene04609:CDS |
| GGTGATTCAGTGAAGTCTAC+GGG | 0.564571 | 7.3:+17601008 | MS.gene04609:CDS |
| TGGGTAGAGGTCGAGGAAAG+GGG | 0.567019 | 7.3:+17600694 | MS.gene04609:CDS |
| GAAGCAATCTGCAATTGTTG+AGG | 0.568281 | 7.3:+17600719 | MS.gene04609:CDS |
| GAAAAGGAAGAGGTCTTCAC+AGG | 0.568389 | 7.3:+17600926 | MS.gene04609:CDS |
| TATGGGTAGAGGTCGAGGAA+AGG | 0.570405 | 7.3:+17600692 | None:intergenic |
| GTCCGAGCTAAGTCTAGCCC+TGG | 0.574363 | 7.3:+17600987 | MS.gene04609:CDS |
| GGTTCTTTGACTATGGGTAG+AGG | 0.582744 | 7.3:+17600681 | None:intergenic |
| TAATGGTGATCTGAAAACTC+AGG | 0.592217 | 7.3:+17600884 | MS.gene04609:CDS |
| GAGACCATTAAAGCCGGTCA+CGG | 0.602292 | 7.3:+17600791 | MS.gene04609:CDS |
| AAGAGGGAGACCATTAAAGC+CGG | 0.607745 | 7.3:+17600785 | MS.gene04609:CDS |
| GTAGACTTCACTGAATCACC+AGG | 0.624371 | 7.3:-17601005 | None:intergenic |
| TGAAAAGATCGAGAAAGATG+AGG | 0.635309 | 7.3:+17600839 | MS.gene04609:CDS |
| CTACACCAGCTTCAGCAGCA+CGG | 0.678409 | 7.3:-17601070 | None:intergenic |
| CCAGCTTCAGCAGCACGGTG+AGG | 0.706272 | 7.3:-17601065 | None:intergenic |
| TAGACTTCACTGAATCACCA+GGG | 0.745429 | 7.3:-17601004 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAAATGGTAATGTTTCTAA+TGG | + | chr7.3:17600867-17600886 | MS.gene04609:CDS | 25.0% |
| AAAGATAGAACCAATGAAAG+AGG | + | chr7.3:17600956-17600975 | MS.gene04609:CDS | 30.0% | |
| AGGTAATCACTGTGAATAAA+AGG | + | chr7.3:17600904-17600923 | MS.gene04609:CDS | 30.0% | |
| TCACTGTGAATAAAAGGAAA+AGG | + | chr7.3:17600910-17600929 | MS.gene04609:CDS | 30.0% | |
| TGAATAAAAGGAAAAGGAAG+AGG | + | chr7.3:17600916-17600935 | MS.gene04609:CDS | 30.0% | |
| AAAGATGAGGAGAACGTAAA+TGG | + | chr7.3:17600852-17600871 | MS.gene04609:CDS | 35.0% | |
| GAACCAATGAAAGAGGAAAA+TGG | + | chr7.3:17600963-17600982 | MS.gene04609:CDS | 35.0% | |
| TAATGGTGATCTGAAAACTC+AGG | + | chr7.3:17600884-17600903 | MS.gene04609:CDS | 35.0% | |
| TGAAAAGATCGAGAAAGATG+AGG | + | chr7.3:17600839-17600858 | MS.gene04609:CDS | 35.0% | |
| ! | ACACCATTTTCCTCTTTCAT+TGG | - | chr7.3:17600969-17600988 | None:intergenic | 35.0% |
| GAAGCAATCTGCAATTGTTG+AGG | + | chr7.3:17600719-17600738 | MS.gene04609:CDS | 40.0% | |
| TACCAGCTCACAGAAAAAGA+GGG | + | chr7.3:17600769-17600788 | MS.gene04609:CDS | 40.0% | |
| TAGACTTCACTGAATCACCA+GGG | - | chr7.3:17601007-17601026 | None:intergenic | 40.0% | |
| TGGTGATTCAGTGAAGTCTA+CGG | + | chr7.3:17601007-17601026 | MS.gene04609:CDS | 40.0% | |
| AAGAGGGAGACCATTAAAGC+CGG | + | chr7.3:17600785-17600804 | MS.gene04609:CDS | 45.0% | |
| GAAAAGGAAGAGGTCTTCAC+AGG | + | chr7.3:17600926-17600945 | MS.gene04609:CDS | 45.0% | |
| GGTGATTCAGTGAAGTCTAC+GGG | + | chr7.3:17601008-17601027 | MS.gene04609:CDS | 45.0% | |
| GTACCAGCTCACAGAAAAAG+AGG | + | chr7.3:17600768-17600787 | MS.gene04609:CDS | 45.0% | |
| GTAGACTTCACTGAATCACC+AGG | - | chr7.3:17601008-17601027 | None:intergenic | 45.0% | |
| TCTACGGGATTCCGACAAAA+TGG | + | chr7.3:17601023-17601042 | MS.gene04609:CDS | 45.0% | |
| ! | TCTTCGTTTTCATCCGTGAC+CGG | - | chr7.3:17600807-17600826 | None:intergenic | 45.0% |
| ATGGGTAGAGGTCGAGGAAA+GGG | + | chr7.3:17600693-17600712 | MS.gene04609:CDS | 50.0% | |
| GAGACCATTAAAGCCGGTCA+CGG | + | chr7.3:17600791-17600810 | MS.gene04609:CDS | 50.0% | |
| GATTCCGACAAAATGGCAGC+AGG | + | chr7.3:17601030-17601049 | MS.gene04609:CDS | 50.0% | |
| ! | CTCCCTCTTTTTCTGTGAGC+TGG | - | chr7.3:17600774-17600793 | None:intergenic | 50.0% |
| ! | TCATCCGTGACCGGCTTTAA+TGG | - | chr7.3:17600798-17600817 | None:intergenic | 50.0% |
| !!! | TACGCCTGCTGCCATTTTGT+CGG | - | chr7.3:17601037-17601056 | None:intergenic | 50.0% |
| CACCAGGGCTAGACTTAGCT+CGG | - | chr7.3:17600992-17601011 | None:intergenic | 55.0% | |
| CTACACCAGCTTCAGCAGCA+CGG | - | chr7.3:17601073-17601092 | None:intergenic | 55.0% | |
| TGGGTAGAGGTCGAGGAAAG+GGG | + | chr7.3:17600694-17600713 | MS.gene04609:CDS | 55.0% | |
| GTCCGAGCTAAGTCTAGCCC+TGG | + | chr7.3:17600987-17601006 | MS.gene04609:CDS | 60.0% | |
| CCAGCTTCAGCAGCACGGTG+AGG | - | chr7.3:17601068-17601087 | None:intergenic | 65.0% | |
| ! | CCTCACCGTGCTGCTGAAGC+TGG | + | chr7.3:17601065-17601084 | MS.gene04609:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 17600693 | 17601103 | 17600693 | ID=MS.gene04609 |
| chr7.3 | mRNA | 17600693 | 17601103 | 17600693 | ID=MS.gene04609.t1;Parent=MS.gene04609 |
| chr7.3 | exon | 17600693 | 17601103 | 17600693 | ID=MS.gene04609.t1.exon1;Parent=MS.gene04609.t1 |
| chr7.3 | CDS | 17600693 | 17601103 | 17600693 | ID=cds.MS.gene04609.t1;Parent=MS.gene04609.t1 |
| Gene Sequence |
| Protein sequence |
