Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072389.t1 | XP_003625240.2 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 2.60E-64 | 254.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072389.t1 | G7KVB6 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 1.9e-64 | 254.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051719 | MS.gene072389 | 0.821431 | 4.05E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene072389 | 0.80508 | 1.59E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene072389 | 0.807303 | 5.40E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene31060 | MS.gene072389 | PPI |
| MS.gene017971 | MS.gene072389 | PPI |
| MS.gene78355 | MS.gene072389 | PPI |
| MS.gene032141 | MS.gene072389 | PPI |
| MS.gene04609 | MS.gene072389 | PPI |
| MS.gene52644 | MS.gene072389 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072389.t1 | MTR_7g092970 | 98.529 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 9.50e-94 | 266 |
| MS.gene072389.t1 | MTR_2g098560 | 58.394 | 137 | 53 | 4 | 1 | 136 | 1 | 134 | 6.57e-40 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072389.t1 | AT3G57930 | 51.748 | 143 | 56 | 5 | 1 | 135 | 1 | 138 | 1.09e-26 | 97.4 |
| MS.gene072389.t1 | AT3G57930 | 51.748 | 143 | 56 | 5 | 1 | 135 | 1 | 138 | 1.09e-26 | 97.4 |
| MS.gene072389.t1 | AT2G42190 | 45.652 | 138 | 72 | 2 | 1 | 135 | 1 | 138 | 3.61e-22 | 85.9 |
| MS.gene072389.t1 | AT2G42190 | 45.652 | 138 | 72 | 2 | 1 | 135 | 1 | 138 | 3.61e-22 | 85.9 |
| MS.gene072389.t1 | AT2G42190 | 45.652 | 138 | 72 | 2 | 1 | 135 | 1 | 138 | 3.61e-22 | 85.9 |
Find 29 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCCGTGACCGGCTTTAA+TGG | 0.312272 | 7.4:-15933860 | None:intergenic |
| AGGTAATTACTGTGAATAAA+AGG | 0.319727 | 7.4:+15933969 | MS.gene072389:CDS |
| AAAGATGAGGAGAACGTAAA+TGG | 0.358180 | 7.4:+15933917 | MS.gene072389:CDS |
| TCTACGGGATTCCGACAAAA+TGG | 0.386285 | 7.4:+15934088 | MS.gene072389:CDS |
| GAACCGATGAAAGAGGAAAA+TGG | 0.394483 | 7.4:+15934028 | MS.gene072389:CDS |
| ATGGGTAGAGGTCGAGGAAA+GGG | 0.429359 | 7.4:+15933758 | MS.gene072389:CDS |
| GTAAATGGTAATGTTTCTAA+TGG | 0.448458 | 7.4:+15933932 | MS.gene072389:CDS |
| CCTCACCGTGCTGCTGAAGC+TGG | 0.469138 | 7.4:+15934130 | MS.gene072389:CDS |
| TTACTGTGAATAAAAGGAAA+AGG | 0.499797 | 7.4:+15933975 | MS.gene072389:CDS |
| TTGACTATGGGTAGAGGTCG+AGG | 0.519155 | 7.4:+15933752 | None:intergenic |
| TGGTGATTCAGTGAAGTCTA+CGG | 0.537967 | 7.4:+15934072 | MS.gene072389:CDS |
| AAAGATAGAACCGATGAAAG+AGG | 0.540072 | 7.4:+15934021 | MS.gene072389:CDS |
| CACCAGGGCTAGACTTAGCT+CGG | 0.543586 | 7.4:-15934054 | None:intergenic |
| GATTCCGACAAAATGGCAGC+AGG | 0.563142 | 7.4:+15934095 | MS.gene072389:CDS |
| GGTGATTCAGTGAAGTCTAC+GGG | 0.564571 | 7.4:+15934073 | MS.gene072389:CDS |
| TGGGTAGAGGTCGAGGAAAG+GGG | 0.567019 | 7.4:+15933759 | MS.gene072389:CDS |
| GAAGCAATCTGCAATTGTTG+AGG | 0.568281 | 7.4:+15933784 | MS.gene072389:CDS |
| GAAAAGGAAGAGATCTTCAC+AGG | 0.568389 | 7.4:+15933991 | MS.gene072389:CDS |
| TATGGGTAGAGGTCGAGGAA+AGG | 0.570405 | 7.4:+15933757 | None:intergenic |
| GTCCGAGCTAAGTCTAGCCC+TGG | 0.574363 | 7.4:+15934052 | MS.gene072389:CDS |
| GGTTCTTTGACTATGGGTAG+AGG | 0.582744 | 7.4:+15933746 | None:intergenic |
| TAATGGTGATCTGAAAACTC+AGG | 0.592217 | 7.4:+15933949 | MS.gene072389:CDS |
| GAGACCATTAAAGCCGGTCA+CGG | 0.602292 | 7.4:+15933856 | MS.gene072389:CDS |
| AAGAGGGAGACCATTAAAGC+CGG | 0.607745 | 7.4:+15933850 | MS.gene072389:CDS |
| GTAGACTTCACTGAATCACC+AGG | 0.624371 | 7.4:-15934070 | None:intergenic |
| TGAAAAGATCGAGAAAGATG+AGG | 0.635309 | 7.4:+15933904 | MS.gene072389:CDS |
| CTACACCAGCTTCAGCAGCA+CGG | 0.678409 | 7.4:-15934135 | None:intergenic |
| CCAGCTTCAGCAGCACGGTG+AGG | 0.706272 | 7.4:-15934130 | None:intergenic |
| TAGACTTCACTGAATCACCA+GGG | 0.745429 | 7.4:-15934069 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGGTAATTACTGTGAATAAA+AGG | + | chr7.4:15933969-15933988 | MS.gene072389:CDS | 25.0% |
| ! | TTACTGTGAATAAAAGGAAA+AGG | + | chr7.4:15933975-15933994 | MS.gene072389:CDS | 25.0% |
| !! | GTAAATGGTAATGTTTCTAA+TGG | + | chr7.4:15933932-15933951 | MS.gene072389:CDS | 25.0% |
| AAAGATAGAACCGATGAAAG+AGG | + | chr7.4:15934021-15934040 | MS.gene072389:CDS | 35.0% | |
| AAAGATGAGGAGAACGTAAA+TGG | + | chr7.4:15933917-15933936 | MS.gene072389:CDS | 35.0% | |
| TAATGGTGATCTGAAAACTC+AGG | + | chr7.4:15933949-15933968 | MS.gene072389:CDS | 35.0% | |
| TGAAAAGATCGAGAAAGATG+AGG | + | chr7.4:15933904-15933923 | MS.gene072389:CDS | 35.0% | |
| ! | ACACCATTTTCCTCTTTCAT+CGG | - | chr7.4:15934034-15934053 | None:intergenic | 35.0% |
| GAAAAGGAAGAGATCTTCAC+AGG | + | chr7.4:15933991-15934010 | MS.gene072389:CDS | 40.0% | |
| GAACCGATGAAAGAGGAAAA+TGG | + | chr7.4:15934028-15934047 | MS.gene072389:CDS | 40.0% | |
| GAAGCAATCTGCAATTGTTG+AGG | + | chr7.4:15933784-15933803 | MS.gene072389:CDS | 40.0% | |
| TACCAGCTCACAGAAAAAGA+GGG | + | chr7.4:15933834-15933853 | MS.gene072389:CDS | 40.0% | |
| TAGACTTCACTGAATCACCA+GGG | - | chr7.4:15934072-15934091 | None:intergenic | 40.0% | |
| TGGTGATTCAGTGAAGTCTA+CGG | + | chr7.4:15934072-15934091 | MS.gene072389:CDS | 40.0% | |
| AAGAGGGAGACCATTAAAGC+CGG | + | chr7.4:15933850-15933869 | MS.gene072389:CDS | 45.0% | |
| GGTGATTCAGTGAAGTCTAC+GGG | + | chr7.4:15934073-15934092 | MS.gene072389:CDS | 45.0% | |
| GTACCAGCTCACAGAAAAAG+AGG | + | chr7.4:15933833-15933852 | MS.gene072389:CDS | 45.0% | |
| GTAGACTTCACTGAATCACC+AGG | - | chr7.4:15934073-15934092 | None:intergenic | 45.0% | |
| TCTACGGGATTCCGACAAAA+TGG | + | chr7.4:15934088-15934107 | MS.gene072389:CDS | 45.0% | |
| ! | TCTTCGTTTTCATCCGTGAC+CGG | - | chr7.4:15933872-15933891 | None:intergenic | 45.0% |
| ATGGGTAGAGGTCGAGGAAA+GGG | + | chr7.4:15933758-15933777 | MS.gene072389:CDS | 50.0% | |
| GAGACCATTAAAGCCGGTCA+CGG | + | chr7.4:15933856-15933875 | MS.gene072389:CDS | 50.0% | |
| GATTCCGACAAAATGGCAGC+AGG | + | chr7.4:15934095-15934114 | MS.gene072389:CDS | 50.0% | |
| ! | CTCCCTCTTTTTCTGTGAGC+TGG | - | chr7.4:15933839-15933858 | None:intergenic | 50.0% |
| ! | TCATCCGTGACCGGCTTTAA+TGG | - | chr7.4:15933863-15933882 | None:intergenic | 50.0% |
| !!! | TACGCCTGCTGCCATTTTGT+CGG | - | chr7.4:15934102-15934121 | None:intergenic | 50.0% |
| CACCAGGGCTAGACTTAGCT+CGG | - | chr7.4:15934057-15934076 | None:intergenic | 55.0% | |
| CTACACCAGCTTCAGCAGCA+CGG | - | chr7.4:15934138-15934157 | None:intergenic | 55.0% | |
| TGGGTAGAGGTCGAGGAAAG+GGG | + | chr7.4:15933759-15933778 | MS.gene072389:CDS | 55.0% | |
| GTCCGAGCTAAGTCTAGCCC+TGG | + | chr7.4:15934052-15934071 | MS.gene072389:CDS | 60.0% | |
| CCAGCTTCAGCAGCACGGTG+AGG | - | chr7.4:15934133-15934152 | None:intergenic | 65.0% | |
| ! | CCTCACCGTGCTGCTGAAGC+TGG | + | chr7.4:15934130-15934149 | MS.gene072389:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 15933758 | 15934168 | 15933758 | ID=MS.gene072389 |
| chr7.4 | mRNA | 15933758 | 15934168 | 15933758 | ID=MS.gene072389.t1;Parent=MS.gene072389 |
| chr7.4 | exon | 15933758 | 15934168 | 15933758 | ID=MS.gene072389.t1.exon1;Parent=MS.gene072389.t1 |
| chr7.4 | CDS | 15933758 | 15934168 | 15933758 | ID=cds.MS.gene072389.t1;Parent=MS.gene072389.t1 |
| Gene Sequence |
| Protein sequence |
