Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78355.t1 | XP_003597485.1 | 97.8 | 134 | 3 | 0 | 1 | 134 | 1 | 134 | 5.00E-60 | 240.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78355.t1 | G7IHR4 | 97.8 | 134 | 3 | 0 | 1 | 134 | 1 | 134 | 3.6e-60 | 240.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056752 | MS.gene78355 | 0.802274 | 6.06E-49 | -1.69E-46 |
| MS.gene06011 | MS.gene78355 | 0.800201 | 1.61E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene032141 | MS.gene78355 | PPI |
| MS.gene78355 | MS.gene52644 | PPI |
| MS.gene78355 | MS.gene032141 | PPI |
| MS.gene78355 | MS.gene072389 | PPI |
| MS.gene04609 | MS.gene78355 | PPI |
| MS.gene78355 | MS.gene04609 | PPI |
| MS.gene78355 | MS.gene017971 | PPI |
| MS.gene78355 | MS.gene31060 | PPI |
| MS.gene52644 | MS.gene78355 | PPI |
| MS.gene017971 | MS.gene78355 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78355.t1 | MTR_2g098560 | 97.761 | 134 | 3 | 0 | 1 | 134 | 1 | 134 | 7.30e-91 | 259 |
| MS.gene78355.t1 | MTR_7g092970 | 57.246 | 138 | 53 | 4 | 1 | 134 | 1 | 136 | 1.87e-39 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78355.t1 | AT3G57930 | 53.846 | 143 | 51 | 5 | 1 | 133 | 1 | 138 | 7.40e-30 | 105 |
| MS.gene78355.t1 | AT3G57930 | 53.846 | 143 | 51 | 5 | 1 | 133 | 1 | 138 | 7.40e-30 | 105 |
| MS.gene78355.t1 | AT2G42190 | 48.921 | 139 | 64 | 3 | 1 | 133 | 1 | 138 | 1.42e-23 | 89.4 |
| MS.gene78355.t1 | AT2G42190 | 48.921 | 139 | 64 | 3 | 1 | 133 | 1 | 138 | 1.42e-23 | 89.4 |
| MS.gene78355.t1 | AT2G42190 | 48.921 | 139 | 64 | 3 | 1 | 133 | 1 | 138 | 1.42e-23 | 89.4 |
Find 28 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGTCAAAGTCGTCCTTTAC+CGG | 0.288385 | 2.3:-4836700 | None:intergenic |
| CAGCGAAATCCAATGGATTT+AGG | 0.362932 | 2.3:+4836902 | MS.gene78355:CDS |
| ATGGGTAGAGGTAGAGGAAA+GGG | 0.421104 | 2.3:+4836586 | MS.gene78355:CDS |
| GATTCAGCTGAGGAAGAAAA+TGG | 0.422625 | 2.3:+4836850 | MS.gene78355:CDS |
| TCCGTTATGCCTAAATCCAT+TGG | 0.436331 | 2.3:-4836911 | None:intergenic |
| CAGCAGAACTTGCAAGGAAA+AGG | 0.444441 | 2.3:+4836797 | MS.gene78355:CDS |
| AAGAGGGAGACCACAGAAAC+CGG | 0.451752 | 2.3:+4836681 | MS.gene78355:CDS |
| GACAGTGAGAATGTTAAGAA+TGG | 0.453819 | 2.3:+4836742 | MS.gene78355:CDS |
| CTTGCAAGTTCTGCTGCTGT+AGG | 0.470856 | 2.3:-4836790 | None:intergenic |
| CTCCACTTACAGTGTCGTCT+TGG | 0.474047 | 2.3:-4836630 | None:intergenic |
| AATATCATGGGTAGAGGTAG+AGG | 0.478296 | 2.3:+4836580 | None:intergenic |
| CCTCGTAGAGCTGCTGAAGC+TGG | 0.484805 | 2.3:+4836952 | MS.gene78355:CDS |
| GTTCCAATACAGAAGAAAAG+AGG | 0.492864 | 2.3:+4836664 | MS.gene78355:CDS |
| CTGACTCCTCTGTGCTTGAT+CGG | 0.502660 | 2.3:-4836882 | None:intergenic |
| TCCAATGGATTTAGGCATAA+CGG | 0.518583 | 2.3:+4836910 | MS.gene78355:CDS |
| AACCAAGACGACACTGTAAG+TGG | 0.527625 | 2.3:+4836628 | MS.gene78355:CDS |
| TGTAAGTGGAGAAGATGAAA+AGG | 0.533937 | 2.3:+4836642 | MS.gene78355:CDS |
| TCGTCCTTTACCGGTTTCTG+TGG | 0.538924 | 2.3:-4836691 | None:intergenic |
| GAGGAGTCAGCGAAATCCAA+TGG | 0.541722 | 2.3:+4836895 | MS.gene78355:CDS |
| TTCCAATACAGAAGAAAAGA+GGG | 0.543516 | 2.3:+4836665 | MS.gene78355:CDS |
| CATGGGTAGAGGTAGAGGAA+AGG | 0.551133 | 2.3:+4836585 | None:intergenic |
| GAAAAGGAAAAGAAACTCAC+AGG | 0.566451 | 2.3:+4836813 | MS.gene78355:CDS |
| GCTGAGGAAGAAAATGGCGT+TGG | 0.572860 | 2.3:+4836856 | MS.gene78355:CDS |
| GAGACCACAGAAACCGGTAA+AGG | 0.577992 | 2.3:+4836687 | MS.gene78355:CDS |
| CTACAGCAGCAGAACTTGCA+AGG | 0.583011 | 2.3:+4836791 | MS.gene78355:CDS |
| AGAGAAGTTAGATTCAGCTG+AGG | 0.635071 | 2.3:+4836840 | MS.gene78355:CDS |
| CCAGCTTCAGCAGCTCTACG+AGG | 0.704470 | 2.3:-4836952 | None:intergenic |
| TGGAAGCCGATCAAGCACAG+AGG | 0.757729 | 2.3:+4836876 | MS.gene78355:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCCAATACAGAAGAAAAGA+GGG | + | chr2.3:4836665-4836684 | MS.gene78355:CDS | 30.0% | |
| GAAAAGGAAAAGAAACTCAC+AGG | + | chr2.3:4836813-4836832 | MS.gene78355:CDS | 35.0% | |
| GACAGTGAGAATGTTAAGAA+TGG | + | chr2.3:4836742-4836761 | MS.gene78355:CDS | 35.0% | |
| GTTCCAATACAGAAGAAAAG+AGG | + | chr2.3:4836664-4836683 | MS.gene78355:CDS | 35.0% | |
| TGTAAGTGGAGAAGATGAAA+AGG | + | chr2.3:4836642-4836661 | MS.gene78355:CDS | 35.0% | |
| ! | TCCAATGGATTTAGGCATAA+CGG | + | chr2.3:4836910-4836929 | MS.gene78355:CDS | 35.0% |
| AGAGAAGTTAGATTCAGCTG+AGG | + | chr2.3:4836840-4836859 | MS.gene78355:CDS | 40.0% | |
| GATTCAGCTGAGGAAGAAAA+TGG | + | chr2.3:4836850-4836869 | MS.gene78355:CDS | 40.0% | |
| TCCGTTATGCCTAAATCCAT+TGG | - | chr2.3:4836914-4836933 | None:intergenic | 40.0% | |
| ! | CAGCGAAATCCAATGGATTT+AGG | + | chr2.3:4836902-4836921 | MS.gene78355:CDS | 40.0% |
| ! | CTCCCTCTTTTCTTCTGTAT+TGG | - | chr2.3:4836670-4836689 | None:intergenic | 40.0% |
| AACCAAGACGACACTGTAAG+TGG | + | chr2.3:4836628-4836647 | MS.gene78355:CDS | 45.0% | |
| ATGGGTAGAGGTAGAGGAAA+GGG | + | chr2.3:4836586-4836605 | MS.gene78355:CDS | 45.0% | |
| CAGCAGAACTTGCAAGGAAA+AGG | + | chr2.3:4836797-4836816 | MS.gene78355:CDS | 45.0% | |
| TCGTCAAAGTCGTCCTTTAC+CGG | - | chr2.3:4836703-4836722 | None:intergenic | 45.0% | |
| AAGAGGGAGACCACAGAAAC+CGG | + | chr2.3:4836681-4836700 | MS.gene78355:CDS | 50.0% | |
| CTACAGCAGCAGAACTTGCA+AGG | + | chr2.3:4836791-4836810 | MS.gene78355:CDS | 50.0% | |
| CTCCACTTACAGTGTCGTCT+TGG | - | chr2.3:4836633-4836652 | None:intergenic | 50.0% | |
| CTGACTCCTCTGTGCTTGAT+CGG | - | chr2.3:4836885-4836904 | None:intergenic | 50.0% | |
| CTTGCAAGTTCTGCTGCTGT+AGG | - | chr2.3:4836793-4836812 | None:intergenic | 50.0% | |
| GAGACCACAGAAACCGGTAA+AGG | + | chr2.3:4836687-4836706 | MS.gene78355:CDS | 50.0% | |
| GAGGAGTCAGCGAAATCCAA+TGG | + | chr2.3:4836895-4836914 | MS.gene78355:CDS | 50.0% | |
| GCTGAGGAAGAAAATGGCGT+TGG | + | chr2.3:4836856-4836875 | MS.gene78355:CDS | 50.0% | |
| !! | TCGTCCTTTACCGGTTTCTG+TGG | - | chr2.3:4836694-4836713 | None:intergenic | 50.0% |
| TGGAAGCCGATCAAGCACAG+AGG | + | chr2.3:4836876-4836895 | MS.gene78355:CDS | 55.0% | |
| CCAGCTTCAGCAGCTCTACG+AGG | - | chr2.3:4836955-4836974 | None:intergenic | 60.0% | |
| CCTCGTAGAGCTGCTGAAGC+TGG | + | chr2.3:4836952-4836971 | MS.gene78355:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 4836586 | 4836990 | 4836586 | ID=MS.gene78355 |
| chr2.3 | mRNA | 4836586 | 4836990 | 4836586 | ID=MS.gene78355.t1;Parent=MS.gene78355 |
| chr2.3 | exon | 4836586 | 4836990 | 4836586 | ID=MS.gene78355.t1.exon1;Parent=MS.gene78355.t1 |
| chr2.3 | CDS | 4836586 | 4836990 | 4836586 | ID=cds.MS.gene78355.t1;Parent=MS.gene78355.t1 |
| Gene Sequence |
| Protein sequence |
